A constant-temperature culture device for microbial fermentation

Abstract

The utility model provides a kind of for microorganism fermentation thermostatic culture device, it is related to microorganism fermentation technical field, including the top cover of being fixed on the upper surface of culture tank, top cover is equipped with stirring subassembly and purification subassembly.The used for microorganism fermentation thermostatic culture device, in the process of microorganism fermentation culture in culture tank, by starting fan, outside air is delivered to air inlet box, air will be in turn through activated carbon layer and carry out adsorption impurity and harmful gas, pass through photocatalyst in sterilization board under the illumination of illuminating lamp and carry out catalytic sterilization, again by ultraviolet lamp, secondary sterilization and disinfection treatment is carried out, ensure that air is completely sterile, finally sterile air is inside culture tank, for microorganism fermentation process continuously provides sterile clean air, promote its normal growth and metabolic activity, ensure the normal progress of fermentation, the light of ultraviolet lamp can be shielded by the baffle of interlaced, avoid ultraviolet light into culture tank to cause influence to microorganism.

Description

Technical Field

[0001] This utility model relates to a culture device, specifically a constant temperature culture device for microbial fermentation, belonging to the field of microbial fermentation technology. Background Technology

[0002] The isothermal fermentation culture of microbial flocculant-producing bacteria requires an isothermal fermentation culture device. Its core is to maintain the optimal activity of the strain through precise temperature control, thereby significantly improving the flocculant yield and quality stability.

[0003] Patent CN220376695U discloses a constant-temperature fermentation culture device for microbial flocculant-producing bacteria. To address the problem of insufficient mixing between air and fermentation culture medium, resulting in low oxygen dissolution in the fermentation culture medium and thus reducing the efficiency of constant-temperature fermentation culture of microbial flocculant-producing bacteria, the internal turbulence channel of the stirring blade has a wide opening at one end and a narrow opening at the other. The stirring blade rotates with the wide opening facing the direction of the turbulence channel, and the liquid inside the cavity flows through the wide opening to the narrow opening of the turbulence channel. As the fluid narrows from wide to narrow, the flow rate increases, thereby improving the stirring efficiency.

[0004] In the above-mentioned patented solution, a one-way exhaust valve is used to maintain positive pressure inside the tank to prevent contamination. However, the device has no air inlet, making it difficult to provide the necessary oxygen for the growth of aerobic microorganisms. Insufficient dissolved oxygen will become a limiting factor for the growth of aerobic bacteria, resulting in low cell density and low product synthesis.

[0005] Therefore, a constant temperature culture device for microbial fermentation is proposed here. Utility Model Content

[0006] This invention proposes a constant temperature culture device for microbial fermentation, which can continuously provide sterile and clean air for the microbial fermentation process, thereby improving the yield and quality stability of the target product.

[0007] This utility model is achieved through the following technical solution: a constant temperature culture device for microbial fermentation, including a top cover fixed on the upper surface of the culture tank, wherein the top cover is provided with a stirring component and a purification component.

[0008] The purification assembly includes an air intake box installed on the upper surface of the top cover. A fan is installed on the upper surface of the air intake box. An activated carbon layer and a sterilization plate are fixed on the inner wall of the air intake box, with the activated carbon layer located above the sterilization plate. Multiple lighting lamps are installed on the inner wall of the air intake box between the activated carbon layer and the sterilization plate. Multiple ultraviolet lamps are installed on the inner wall of the air intake box below the sterilization plate. Two baffles are fixed on the inner wall of the air intake box below the ultraviolet lamps, and the two baffles are staggered.

[0009] Furthermore, an insulation tank is provided on the outside of the culture tank, and an installation ring is fixed on the upper surface of the insulation tank, and the installation ring is connected to the top cover by bolts.

[0010] Furthermore, the inner wall of the heat preservation tank is fixed with multiple mounting blocks, and the other side of the mounting blocks is fixed to the outer surface of the culture tank. An electric heating coil is installed on the mounting block, and the electric heating coil is located between the heat preservation tank and the culture tank.

[0011] Furthermore, a feed pipe is installed on the upper surface of the top cover, and a sealing cap is provided on the feed pipe. A discharge pipe is installed at the bottom of the culture tank, and the bottom end of the discharge pipe extends to the outside of the heat preservation tank. A control valve is provided on the discharge pipe.

[0012] Furthermore, the stirring assembly includes a rotary motor fixed to the upper surface of the top cover, the output shaft of the rotary motor passing through the top cover and fixed with a rotating rod, and a plurality of first stirring rods fixed to the outer surface of the rotating rod.

[0013] Furthermore, a fixed plate is fixed to the bottom end of the rotating rod, and a plurality of second stirring rods are fixed to the outer surface of the fixed plate.

[0014] Furthermore, a temperature sensor is installed inside the culture tank, and a controller is installed on the outer surface of the insulated tank. Both the temperature sensor and the heating coil are electrically connected to the controller.

[0015] This invention provides a constant-temperature culture device for microbial fermentation, which has the following beneficial effects:

[0016] 1. This is a constant temperature culture device for microbial fermentation. By starting a rotary motor, the rotating rod is driven to rotate, which in turn drives the first stirring rod to rotate. The rotation of the first stirring rod can stir the fermentation culture medium in the culture tank. At the same time, the unique axial flow design of the first stirring rod can effectively reduce the generation of bubbles from both the source and process levels. Then, the rotating rod can also drive the second stirring rod to rotate through the fixed plate, thereby avoiding the phenomenon of sedimentation at the bottom of the liquid.

[0017] 2. This is a constant-temperature culture device for microbial fermentation. During the microbial fermentation process in the culture tank, an external air is delivered to the air inlet box by starting a fan. The air passes through an activated carbon layer to adsorb impurities and harmful gases, then through a photocatalyst in a sterilization plate under illumination to catalyze sterilization, and finally through a UV lamp for secondary sterilization and disinfection to ensure that the air is completely sterile. Finally, the sterile air is stored inside the culture tank, continuously providing sterile and clean air for the microbial fermentation process, promoting its normal growth and metabolic activities, and ensuring the normal progress of fermentation. Attached Figure Description

[0018] Figure 1This is a three-dimensional structural diagram of the present invention;

[0019] Figure 2 This is a cross-sectional schematic diagram of the insulated tank, top cover, and mounting ring of this utility model;

[0020] Figure 3 This is a cross-sectional schematic diagram of the top cover and the culture tank in this utility model;

[0021] Figure 4 This is a cross-sectional view of the air intake box in this utility model.

[0022] Explanation of reference numerals in the attached figures

[0023] 1. Insulated container; 101. Mounting ring; 102. Mounting block;

[0024] 2. Purification components; 201. Air intake box; 202. Fan; 203. Activated carbon layer; 204. Lighting lamp; 205. Ultraviolet lamp; 206. Baffle; 207. Sterilization plate;

[0025] 3. Stirring assembly; 301. Rotary motor; 302. Rotating rod; 303. First stirring rod; 304. Fixed plate; 305. Second stirring rod;

[0026] 4. Culture tank; 401. Top cover; 4011. Feed pipe; 402. Discharge pipe;

[0027] 5. Heating coil; 6. Controller; 7. Temperature sensor. Detailed Implementation

[0028] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of the embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of this application.

[0029] Please see Figures 1-4 The present invention proposes the following implementation scheme: a constant temperature culture device for microbial fermentation, including a top cover 401 fixed on the upper surface of a culture tank 4, and a stirring component 3 and a purification component 2 provided on the top cover 401.

[0030] The culture tank 4 is equipped with an insulation tank 1 on its exterior. An installation ring 101 is fixed on the upper surface of the insulation tank 1, and the installation ring 101 is connected to the top cover 401 by bolts.

[0031] A feed pipe 4011 is installed on the upper surface of the top cover 401, and a sealing cover is provided on the feed pipe 4011. A discharge pipe 402 is installed at the bottom of the culture tank 4, and the bottom end of the discharge pipe 402 extends to the outside of the heat preservation tank 1. A control valve is provided on the discharge pipe 402.

[0032] Please refer to this carefully. Figure 3 and Figure 4 The purification component 2 includes an air intake box 201 installed on the upper surface of the top cover 401. A fan 202 is installed on the upper surface of the air intake box 201. An activated carbon layer 203 and a sterilization plate 207 are fixed on the inner wall of the air intake box 201, with the activated carbon layer 203 located above the sterilization plate 207. Multiple lighting lamps 204 are installed on the inner wall of the air intake box 201 between the activated carbon layer 203 and the sterilization plate 207. Multiple ultraviolet lamps 205 are installed on the inner wall of the air intake box 201 below the sterilization plate 207. Two baffles 206 are fixed on the inner wall of the air intake box 201 below the ultraviolet lamps 205, and the two baffles 206 are staggered.

[0033] During the microbial fermentation process in the culture tank 4, external air is delivered to the air inlet box 201 by starting the fan 202. The air passes through the activated carbon layer 203 to adsorb impurities and harmful gases, then through the photocatalyst in the sterilization plate 207 for catalytic sterilization under the illumination of the lamp 204, and then through the ultraviolet lamp 205 for secondary sterilization and disinfection to ensure that the air is completely sterile. Finally, the sterile air enters the interior of the culture tank 4, continuously providing sterile and clean air for the microbial fermentation process, promoting its normal growth and metabolic activities, and ensuring the normal progress of fermentation. The interlocking baffles 206 can block the light from the ultraviolet lamp 205 to prevent ultraviolet light from entering the culture tank 4 and affecting the microorganisms.

[0034] Please refer to this carefully. Figure 2 and Figure 3 Multiple mounting blocks 102 are fixed to the inner wall of the heat preservation tank 1, and the other side of the mounting block 102 is fixed to the outer surface of the culture tank 4. An electric heating ring 5 is installed on the mounting block 102, and the electric heating ring 5 is located between the heat preservation tank 1 and the culture tank 4. Through the setting of the electric heating ring 5, the fermentation culture medium in the culture tank 4 can be heated to keep it at a constant temperature.

[0035] A temperature sensor 7 is installed inside the culture tank 4, and a controller 6 is installed on the outer surface of the insulated tank 1. Both the temperature sensor 7 and the heating coil 5 are electrically connected to the controller 6. The temperature sensor 7 is used to collect temperature information inside the culture tank 4 and feed it back to the controller 6. The controller 6 controls the working state of the heating coil 5 based on the temperature information fed back by the temperature sensor 7 to adjust the temperature inside the culture tank 4. The controller 6 has adjustment buttons and a display screen to set the constant temperature and display the temperature inside the culture tank 4 in real time.

[0036] Please refer to this carefully. Figure 3 The stirring assembly 3 includes a rotary motor 301 fixed on the upper surface of the top cover 401. The output shaft end of the rotary motor 301 passes through the top cover 401 and is fixed with a rotating rod 302. Multiple first stirring rods 303 are fixed on the outer surface of the rotating rod 302.

[0037] A fixed plate 304 is fixed to the bottom end of the rotating rod 302, and multiple second stirring rods 305 are fixed to the outer surface of the fixed plate 304.

[0038] By starting the rotary motor 301, the rotating rod 302 is driven to rotate, which in turn drives the first stirring rod 303 to rotate. The rotation of the first stirring rod 303 can stir the fermentation culture in the culture tank 4. At the same time, the unique axial flow design of the first stirring rod 303 can effectively reduce the generation of bubbles from both the source and process levels. Then, the rotating rod 302 can also drive the second stirring rod 305 to rotate through the fixed plate 304, thereby preventing the phenomenon of sedimentation at the bottom of the liquid.

[0039] In use, the raw materials required for fermentation are fed into the culture tank 4 through the feed pipe 4011. Then, the heating coil 5 is activated to heat the fermentation culture medium in the culture tank 4 to maintain a constant temperature. Next, the rotary motor 301 drives the rotating rod 302 to rotate, which in turn drives the first stirring rod 303 to rotate. The rotation of the first stirring rod 303 stirs the fermentation culture medium in the culture tank 4. Simultaneously, the unique axial flow design of the first stirring rod 303 effectively reduces bubble formation from both the source and process levels. Furthermore, the rotating rod 302 also drives the second stirring rod 305 to rotate via the fixed plate 304, thereby preventing sedimentation at the bottom of the liquid. During the microbial fermentation process in the culture tank 4, external air is supplied to the air inlet box 201 by starting the fan 202. The air passes through the activated carbon layer 203 to adsorb impurities and harmful gases, then through the photocatalyst in the sterilization plate 207 under the illumination of the lamp 204 for catalytic sterilization, and then through the ultraviolet lamp 205 for secondary sterilization and disinfection to ensure that the air is completely sterile. Finally, the sterile air is stored inside the culture tank 4, continuously providing sterile and clean air for the microbial fermentation process, promoting its normal growth and metabolic activities, and ensuring the normal progress of fermentation. The interlocking baffles 206 can block the light from the ultraviolet lamp 205 to prevent ultraviolet light from entering the culture tank 4 and affecting the microorganisms.

[0040] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A thermostatic fermentation apparatus for microbial fermentation, comprising a top cover (401) fixed to the upper surface of a culture tank (4), characterized in that: The top cover (401) is equipped with a stirring assembly (3) and a purification assembly (2); The purification component (2) includes an air intake box (201) installed on the upper surface of the top cover (401). A fan (202) is installed on the upper surface of the air intake box (201). An activated carbon layer (203) and a sterilization plate (207) are fixed on the inner wall of the air intake box (201), and the activated carbon layer (203) is located above the sterilization plate (207). Multiple lighting lamps (204) are installed on the inner wall of the air intake box (201) between the activated carbon layer (203) and the sterilization plate (207). Multiple ultraviolet lamps (205) are installed on the inner wall of the air intake box (201) below the sterilization plate (207). Two baffles (206) are fixed on the inner wall of the air intake box (201) below the ultraviolet lamps (205), and the two baffles (206) are staggered.

2. The isothermal culture device for microbial fermentation according to claim 1, characterized in that: The culture tank (4) is provided with an insulated tank (1) on its exterior. An installation ring (101) is fixed on the upper surface of the insulated tank (1), and the installation ring (101) is connected to the top cover (401) by bolts.

3. The isothermal culture device for microbial fermentation according to claim 2, characterized in that: The inner wall of the heat preservation tank (1) is fixed with a plurality of mounting blocks (102), and the other side of the mounting block (102) is fixed on the outer surface of the culture tank (4). The mounting block (102) is equipped with an electric heating coil (5), and the electric heating coil (5) is located between the heat preservation tank (1) and the culture tank (4).

4. The isothermal culture device for microbial fermentation according to claim 1, characterized in that: The top cover (401) is equipped with a feed pipe (4011) on its upper surface and a sealing cap is provided on the feed pipe (4011). The bottom end of the culture tank (4) is equipped with a discharge pipe (402) and the bottom end of the discharge pipe (402) extends to the outside of the heat preservation tank (1). A control valve is provided on the discharge pipe (402).

5. The isothermal culture device for microbial fermentation according to claim 1, characterized in that: The stirring assembly (3) includes a rotary motor (301) fixed on the upper surface of the top cover (401). The output shaft end of the rotary motor (301) passes through the top cover (401) and is fixed with a rotating rod (302). A plurality of first stirring rods (303) are fixed on the outer surface of the rotating rod (302).

6. The isothermal culture device for microbial fermentation according to claim 5, characterized in that: The bottom end of the rotating rod (302) is fixed with a fixed plate (304), and a plurality of second stirring rods (305) are fixed on the outer surface of the fixed plate (304).

7. The isothermal culture device for microbial fermentation according to claim 3, characterized in that: A temperature sensor (7) is installed inside the culture tank (4), and a controller (6) is installed on the outer surface of the heat preservation tank (1). The temperature sensor (7) and the heating coil (5) are both electrically connected to the controller (6).

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