A three-dimensional cell culture device

Abstract

The utility model discloses a three -dimensional cell culture device, including the fixed plate, the upper surface of fixed plate is connected with the support frame, the upper surface of support frame is inlayed with the culture cylinder, the bottom surface of culture cylinder is inlayed with the stirring mechanism, the right side of fixed plate is connected with the cell temporary storage cylinder. The device passes through the design of driving motor and sealing bearing, and it is convenient for driving connecting rod to rotate, and according to the connecting relationship of connecting rod and stirring board, it is convenient for stirring board to follow the rotation of connecting rod and carry out the stirring shearing of cell, utilize the design of conveying hose, and it is convenient for the cell in culture cylinder to flow into the inside of cell temporary storage cylinder, then through the design of liquid peristaltic pump and liquid pipet, it is convenient for sucking the cell in cell temporary storage cylinder, and utilize liquid delivery pipe and be convenient for stirring board to circulate and carry out the stirring shearing of cell, and then it is favorable to fully stir cell, and the efficiency of cell culture has been improved.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and in particular to a three-dimensional cell culture device. Background Technology

[0002] Cell culture refers to the technique of taking cells from an organism and simulating the in vivo environment to enable them to survive and proliferate. It is widely used in fields such as biology, medicine, and pharmacy, and is one of the fundamental methods of cell biology research.

[0003] Current cell culture devices typically employ a single stirring structure to agitate and shear cells, aiming to accelerate cell culture efficiency. However, single-level stirring only achieves radial planar mixing and cannot lift cells from the bottom to the top, leading to cell sedimentation and accumulation, insufficient mixing, and poor culture results. Therefore, we propose a three-dimensional cell culture device to address these issues. Utility Model Content

[0004] The purpose of this invention is to provide a three-dimensional cell culture device that uses a composite system combining stirring and shearing with extracorporeal circulation to achieve thorough mixing in three-dimensional space and improve cell culture efficiency.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] A three-dimensional cell culture device includes a fixed plate, a support frame connected to the upper surface of the fixed plate, a culture tube embedded in the upper surface of the support frame, a stirring mechanism embedded in the bottom surface of the culture tube, a cell storage tube connected to the right side of the fixed plate, a conveying mechanism connected to the upper surface of the cell storage tube, the lower part of the outer surface of the culture tube communicating with the outer surface of the cell storage tube through a conveying hose, and the output end of the conveying mechanism communicating with the upper part of the culture tube, forming a vertical circulation path.

[0007] Furthermore, the stirring mechanism includes a sealed bearing embedded in the culture cylinder, a connecting rod connected to the inner ring of the sealed bearing, a stirring plate connected to the top end of the connecting rod, a drive motor connected to the bottom end of the connecting rod, and the bottom surface of the drive motor connected to the upper surface of the fixed plate.

[0008] Furthermore, the delivery mechanism includes a liquid peristaltic pump connected to the cell storage cylinder. The two ends of the liquid peristaltic pump are respectively connected to a liquid pipette and a liquid delivery tube. One end of the liquid delivery tube is connected to the upper part of the outer surface of the culture cylinder, and one end of the liquid pipette passes through the cell storage cylinder and extends into the interior of the cell storage cylinder.

[0009] Furthermore, a process display board is provided above the fixed plate, and the back of the process display board is connected to the front of the support frame.

[0010] Furthermore, a storage box is connected to the upper surface of the fixing plate, and the storage box is located in front of the cell storage cylinder.

[0011] Furthermore, a control panel is provided at the front of the support frame, and the back of the control panel is connected to the front of the support frame.

[0012] Compared with the prior art, the beneficial effects of this utility model are:

[0013] This device employs a combined culture system integrating a stirring mechanism and a transport mechanism: the stirring mechanism, driven by a motor, rotates a stirring plate to horizontally agitate and shear the cell suspension, breaking up cell clumps; the transport mechanism, driven by a peristaltic pump, extracts the cell suspension from the bottom of the culture vessel and pumps it back in from the top, forming a vertical axial circulation that continuously lifts cells from the bottom to the top, preventing sedimentation and accumulation. The combined effect of these two mechanisms achieves thorough mixing in three dimensions, resulting in more uniform mixing and more complete culture compared to a single stirring structure, effectively improving the efficiency and quality of cell culture. Attached Figure Description

[0014] Figure 1 is a schematic diagram of the overall three-dimensional structure of the three-dimensional cell culture device;

[0015] Figure 2 is a sectional view of the support frame of the three-dimensional cell culture device from the side.

[0016] Figure 3 is a top view of the three-dimensional structure of the fixing plate of the three-dimensional cell culture device;

[0017] Figure 4 is a side view of the three-dimensional structure of the fixation plate of the three-dimensional cell culture device.

[0018] In the diagram: 1. Delivery mechanism; 101. Liquid peristaltic pump; 102. Liquid delivery pipe; 103. Liquid pipette; 2. Fixing plate; 3. Cell storage cylinder; 4. Culture cylinder; 5. Support frame; 6. Stirring mechanism; 601. Sealed bearing; 602. Connecting rod; 603. Drive motor; 604. Stirring plate; 7. Process display board; 8. Control panel; 9. Delivery hose; 10. Storage box. Detailed Implementation

[0019] In the description of this utility model, it should be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientation or positional relationships, are based on the orientation or positional relationships shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined with "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, unless otherwise stated, "a plurality of" means two or more.

[0020] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0022] Please refer to Figures 1-4. In this utility model, a three-dimensional cell culture device includes a fixing plate 2. A support frame 5 is connected to the upper surface of the fixing plate 2. A culture tube 4 is embedded in the upper surface of the support frame 5. A stirring mechanism 6 is embedded in the bottom surface of the culture tube 4. A cell storage tube 3 is connected to the right side of the fixing plate 2. A conveying mechanism 1 is connected to the upper surface of the cell storage tube 3. The lower part of the outer surface of the culture tube 4 is connected to the outer surface of the cell storage tube 3 through a conveying hose 9. The output end of the conveying mechanism 1 is connected to the upper part of the culture tube 4, together forming an in vitro circulation path.

[0023] The stirring mechanism 6 includes a sealed bearing 601 embedded in the culture cylinder 4. A connecting rod 602 is connected to the inner ring of the sealed bearing 601. A stirring plate 604 is connected to the top end of the connecting rod 602, and a drive motor 603 is connected to the bottom end of the connecting rod 602. The bottom surface of the drive motor 603 is connected to the upper surface of the fixed plate 2. The drive motor 603 drives the stirring plate 604 to rotate horizontally via the connecting rod 602, performing radial shear stirring of the cell suspension and breaking up cell aggregates.

[0024] The delivery mechanism 1 includes a liquid peristaltic pump 101 connected to the cell storage cylinder 3. The two ends of the liquid peristaltic pump 101 are respectively connected to a liquid pipette 103 and a liquid delivery tube 102. One end of the liquid delivery tube 102 is connected to the upper outer surface of the culture cylinder 4, and one end of the liquid pipette 103 penetrates the cell storage cylinder 3 and extends into its interior. The liquid peristaltic pump 101 provides circulation power, introducing the cell suspension from the bottom of the culture cylinder 4 into the cell storage cylinder 3 via the delivery tube 9, then aspirating it and injecting it back from the top of the culture cylinder 4, thus achieving a rising and circulating circulation of liquid from the bottom to the top.

[0025] A process display board 7 is provided above the fixed plate 2. The back of the process display board 7 is connected to the front of the support frame 5 and is used to display the operation process of the device. A storage box 10 is connected to the upper surface of the fixed plate 2. The storage box 10 is located in front of the cell storage cylinder 3 and can store experimental consumables. A control panel 8 is provided in front of the support frame 5. The back of the control panel 8 is connected to the front of the support frame 5 and is used to control the start and stop of the device and adjust the parameters.

[0026] Working principle:

[0027] In use, the cell suspension is injected into the culture tube 4 and the device is started. The drive motor 603 drives the connecting rod 602 to rotate within the sealed bearing 601, which in turn drives the stirring plate 604 to rotate horizontally, performing horizontal stirring and shearing on the cell suspension, breaking up cell clumps, and achieving uniform mixing in the radial plane.

[0028] Simultaneously, the liquid peristaltic pump 101 is activated to generate suction power, and the cell suspension at the bottom of the culture tube 4 flows into the cell storage tube 3 through the delivery hose 9. The liquid pipette 103 draws the cell suspension in the storage tube and injects it back into the culture tube 4 from the top through the liquid delivery tube 102, continuously raising the cell suspension at the bottom to the top, forming a vertical axial circulation.

[0029] The combination of horizontal stirring and shearing with vertical axial circulation allows the cell suspension to flow and mix continuously in three-dimensional space. This avoids cell sedimentation and accumulation while ensuring full contact throughout the entire area. Compared to a single stirring structure, the mixing is more uniform and thorough, effectively improving the efficiency and quality of cell culture.

[0030] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0031] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A three-dimensional cell culture device, characterized in that: The device includes a fixing plate (2), a support frame (5) connected to the upper surface of the fixing plate (2), a culture tube (4) embedded in the upper surface of the support frame (5), a stirring mechanism (6) embedded in the bottom surface of the culture tube (4), a cell storage tube (3) connected to the right side of the fixing plate (2), a conveying mechanism (1) connected to the upper surface of the cell storage tube (3), and the outer surface of the culture tube (4) connected to the outer surface of the cell storage tube (3) through a conveying hose (9).

2. The three-dimensional cell culture device according to claim 1, characterized in that: The stirring mechanism (6) includes a sealed bearing (601) embedded in the culture cylinder (4). The inner ring of the sealed bearing (601) is connected to a connecting rod (602). The top end of the connecting rod (602) is connected to a stirring plate (604). The bottom end of the connecting rod (602) is connected to a drive motor (603). The bottom surface of the drive motor (603) is connected to the upper surface of the fixing plate (2).

3. The three-dimensional cell culture device according to claim 1, characterized in that: The delivery mechanism (1) includes a liquid peristaltic pump (101) connected to the cell storage cylinder (3). The two ends of the liquid peristaltic pump (101) are respectively connected to a liquid pipette (103) and a liquid delivery tube (102). One end of the liquid delivery tube (102) is connected to the outer surface of the culture cylinder (4), and one end of the liquid pipette (103) penetrates the cell storage cylinder (3) and extends into the interior of the cell storage cylinder (3).

4. The three-dimensional cell culture device according to claim 1, characterized in that: A process display board (7) is provided above the fixed plate (2), and the back of the process display board (7) is connected to the front of the support frame (5).

5. A three-dimensional cell culture device according to claim 1, characterized in that: The upper surface of the fixing plate (2) is connected to a storage box (10), which is located in front of the cell storage tube (3).

6. The three-dimensional cell culture device according to claim 1, characterized in that: The support frame (5) has a control panel (8) at the front, and the back of the control panel (8) is connected to the front of the support frame (5).

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