A gas circulation system for a cell incubator

Abstract

The utility model relates to cell culture technical field especially is a kind of cell incubator gas circulation system, including culture mechanism, and culture mechanism is equipped with gas circulation mechanism, and culture mechanism includes shell, and the inner wall of shell is equipped with heating groove, and the inside fixed connection of heating groove has electric heating wire, and the outer surface of shell is equipped with air inlet, and the outer surface fixed connection of shell has flange, and the thread connection of flange has screw cap, and the end of screw cap is provided with filter screen, and gas circulation mechanism includes upper cover, and the inner wall fixed connection of upper cover has fixed plate, and the lower surface fixed connection of fixed plate has temperature detection sensor, and the lower surface middle part fixed connection of fixed plate has oxygen concentration detection sensor, and the inner top fixed connection of upper cover has fan, and the top of upper cover is equipped with air hole.The utility model provides a kind of cell incubator gas circulation system, which can accurately control gas composition and effectively maintain the temperature stability in the box.

Description

Technical Field

[0001] This utility model relates to the field of cell culture, and in particular to a gas circulation system for a cell culture chamber. Background Technology

[0002] Cell culture incubators originated from the need to simulate the physiological environment in vivo for in vitro cell culture and have gradually evolved with the development of biomedicine. Their core technology addresses the problems of unstable environment and high risk of contamination in early equipment by using precise temperature control (such as infrared and water-jacketed heating), gas regulation (CO2 and O2 concentration adaptation), aseptic protection (HEPA filtration and high-temperature sterilization), and humidity maintenance. This allows them to meet the standardized and high-quality requirements for cell growth in scientific research, clinical practice, and industrial production.

[0003] When culturing cells, it is important to pay attention to the ratio of oxygen and carbon dioxide in the cell culture incubator. The gas composition in the incubator needs to be adjusted according to the needs of the cells to ensure that the cells can grow in a suitable environment. Traditional cell culture incubators have shortcomings in gas circulation. Most of the gas introduced is room temperature gas (the cell culture temperature is generally around 37°C, while the indoor air temperature is generally below 37°C). When introducing outside air to adjust the gas ratio, the temperature inside the cell culture incubator is prone to drop suddenly. Utility Model Content

[0004] In view of this, the present invention provides a gas circulation system for a cell culture chamber, the main technical problem to be solved being: to provide a gas circulation system for a cell culture chamber that can accurately regulate gas composition and effectively maintain stable temperature inside the chamber.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: a cell culture chamber gas circulation system, including a culture mechanism, on which a gas circulation mechanism is installed. The culture mechanism includes a shell, a heating groove is formed on the inner wall of the shell, an electric heating wire is fixedly connected inside the heating groove, an air inlet is formed on the outer surface of the shell, the air inlet is connected to the heating groove, a flange is fixedly connected to the outer surface of the shell, a threaded cap is threadedly connected to the flange, and a filter screen is provided at the end of the threaded cap. The gas circulation mechanism includes a top cover, a fixing plate is fixedly connected to the inner wall of the top cover, a temperature detection sensor is fixedly connected to the lower surface of the fixing plate, an oxygen concentration detection sensor is fixedly connected to the middle of the lower surface of the fixing plate, a fan is fixedly connected to the inner top of the top cover, and an air exchange port is formed on the top of the top cover, the position of the air exchange port corresponding to the position of the fan.

[0006] By adopting the above technical solution, when the oxygen concentration and carbon dioxide concentration ratio inside the shell are unbalanced, the air inside the shell is discharged from the ventilation port by the fan. At this time, the shell will be in a negative pressure state, and the outside air will enter the shell through the air inlet to replenish it, thereby balancing the oxygen concentration and carbon dioxide concentration ratio inside the shell. When the outside air enters through the air inlet, it will come into contact with the heating wire. The heating wire generates heat when it is energized, which can heat the air entering the shell and prevent a large amount of low-temperature outside air from entering the shell and affecting cell culture.

[0007] As a further description of the above technical solution:

[0008] The top of the outer casing has a mounting groove, and the bottom of the upper cover is located inside the mounting groove.

[0009] By adopting the above technical solution, it is easy to install the top cover onto the outer shell.

[0010] As a further description of the above technical solution:

[0011] A rubber ring is installed inside the mounting groove, and the bottom of the top cover is in contact with the outer surface of the rubber ring.

[0012] By adopting the above technical solution, the sealing between the top cover and the outer shell can be increased through the rubber ring.

[0013] As a further description of the above technical solution:

[0014] A shock-absorbing rubber plate is fixedly connected to the inner bottom of the outer casing.

[0015] By adopting the above technical solution, cell culture equipment is placed on a shock-absorbing rubber plate during use. The shock-absorbing rubber plate can reduce the impact of vibration generated by the fan on the cell culture equipment.

[0016] As a further description of the above technical solution:

[0017] A power source is fixedly connected to the inner top of the cover, and the power source is electrically connected to a temperature detection sensor, an oxygen concentration detection sensor, and a fan.

[0018] By adopting the above technical solution, power is supplied to the temperature detection sensor, oxygen concentration detection sensor, fan, etc.

[0019] As a further description of the above technical solution:

[0020] A partition net is fixedly connected inside the top cover, and the partition net is located between the fixed plate and the fan.

[0021] By adopting the above technical solution, the separator can prevent external impurities from falling into the outer casing from the air vent.

[0022] By employing the above technical solution, the gas circulation system for a cell culture chamber of this utility model has at least the following beneficial effects:

[0023] 1. Compared with existing technologies, this cell culture chamber gas circulation system heats the air entering the outer shell by setting up heating wires, which effectively avoids the problem of sudden temperature drop in the chamber caused by a large amount of low-temperature air entering the chamber when introducing outside air for gas ratio control in traditional cell culture chambers. It can accurately control the gas composition while effectively maintaining the stability of the temperature inside the chamber. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of the overall structure proposed in this utility model;

[0025] Figure 2 This is a first-view sectional view of the internal structure proposed in this utility model;

[0026] Figure 3 This is a first-view sectional view of the internal structure proposed in this utility model;

[0027] Figure 4 The present utility model proposes Figure 3 Enlarged view of the structure at point A in the middle.

[0028] Legend:

[0029] 1. Cultivation Mechanism; 101. Outer Shell; 102. Heating Tank; 103. Heating Wire; 104. Air Inlet; 105. Flange; 106. Threaded Cap; 107. Filter Screen; 108. Mounting Slot; 109. Rubber Ring; 110. Shock-absorbing Rubber Plate; 2. Gas Circulation Mechanism; 201. Top Cover; 202. Power Supply; 203. Separator; 204. Fixing Plate; 205. Temperature Sensor; 206. Oxygen Concentration Sensor; 207. Fan; 208. Ventilation Port. Detailed Implementation

[0030] Reference Figure 1-4This utility model provides a gas circulation system for a cell culture chamber, comprising a culture mechanism 1, a gas circulation mechanism 2 mounted on the culture mechanism 1, a shell 101, a heating groove 102 formed on the inner wall of the shell 101, a heating wire 103 fixedly connected inside the heating groove 102, an air inlet 104 formed on the outer surface of the shell 101, the air inlet 104 communicating with the heating groove 102, a flange 105 fixedly connected to the outer surface of the shell 101, a threaded cap 106 threadedly connected to the flange 105, and a filter screen 107 provided at the end of the threaded cap 106. The gas circulation mechanism 2 includes a top cover 201, a fixing plate 204 fixedly connected to the inner wall of the top cover 201, a temperature sensor 205 fixedly connected to the lower surface of the fixing plate 204 (the temperature sensor 205 can be a PT100), and an oxygen concentration sensor 206 fixedly connected to the middle of the lower surface of the fixing plate 204. The model can be Model902P or other sensor devices capable of detecting oxygen and carbon dioxide concentrations. A fan 207 is fixedly connected to the inner top of the cover 201. An air exchange port 208 is opened on the top of the cover 201, and the position of the air exchange port 208 corresponds to the position of the fan 207. During use, when the oxygen concentration and carbon dioxide concentration ratio inside the shell 101 are unbalanced, the fan 207 operates to exhaust the air inside the shell 101 through the air exchange port 208. At this time, the shell 101 will be in a negative pressure state, and outside air will enter the shell 101 through the air inlet 104 to replenish it, thereby balancing the oxygen concentration and carbon dioxide concentration ratio inside the shell 101. When the outside air enters through the air inlet 104, it will come into contact with the heating wire 103. The heating wire 103 generates heat when it is energized, which can heat the air entering the shell 101 and prevent a large amount of low-temperature outside air from entering the shell 101 and affecting cell culture.

[0031] The top of the outer casing 101 is provided with a mounting groove 108, and the bottom of the upper cover 201 is located inside the mounting groove 108, which facilitates the installation of the upper cover 201 onto the outer casing 101. A rubber ring 109 is installed inside the mounting groove 108, and the bottom of the upper cover 201 fits against the outer surface of the rubber ring 109. The rubber ring 109 can increase the sealing between the upper cover 201 and the outer casing 101. A shock-absorbing rubber plate 110 is fixedly connected to the inner bottom of the outer casing 101. When in use, cell culture equipment and the like are placed on the shock-absorbing rubber plate 110. The shock-absorbing rubber plate 110 can reduce the impact of vibration generated by the fan 207 on the cell culture equipment when it is working. A partition net 203 is fixedly connected inside the upper cover 201. The partition net 203 is located between the fixed plate 204 and the fan 207. The partition net 203 can prevent external impurities from falling into the outer casing 101 from the ventilation port 208.

[0032] A power supply 202 is fixedly connected to the inner top of the cover 201. The power supply 202 is electrically connected to the temperature sensor 205, the oxygen concentration sensor 206, and the fan 207. The power supply 202 supplies power to the temperature sensor 205, the oxygen concentration sensor 206, and the fan 207. The heating wire 103 can be powered by the power supply 202 or by an external power source. In actual use, the fan 207, the temperature sensor 205, the oxygen concentration sensor 206, and the heating wire 103 are coordinated and controlled by a PLC program. When the fan 207 is powered on, it blows the air out of the shell 101, and outside air enters through the air inlet 104. At the same time, the heating wire 103 is powered on to heat the incoming air. The temperature sensor 205 monitors the temperature inside the shell 101 in real time, and the oxygen concentration sensor 206 monitors the oxygen concentration inside the shell 101 in real time.

[0033] Working Principle: When the oxygen concentration sensor detects that the oxygen concentration inside the enclosure is lower than the set threshold, the signal is transmitted to the PLC control system. The PLC program immediately starts the fan and simultaneously activates the heating wire. At this time, the fan operates at a set speed, expelling the air inside the enclosure through the ventilation port to create a negative pressure environment. Outside air enters the heating tank through the air inlet, making full contact with the electrically heated heating wire to complete preheating. The temperature sensor continuously monitors the air temperature after heating. When the required temperature range for cell culture is reached, the PLC program dynamically adjusts the fan speed through the frequency converter, ensuring gas circulation efficiency while avoiding temperature fluctuations exceeding ±0.5℃. The filter screen can intercept particles larger than 0.3μm in diameter, and together with the separator screen, ensures the cleanliness of the air entering the culture environment. When the oxygen concentration returns to the set range, the PLC program automatically reduces the fan power to standby mode, and the heating wire enters a low-power heat preservation mode to maintain the dual stability of gas composition and temperature inside the chamber.

[0034] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A gas circulation system for a cell culture chamber, comprising a culture mechanism (1), characterized in that: The culture mechanism (1) is equipped with a gas circulation mechanism (2). The culture mechanism (1) includes a shell (101). A heating groove (102) is provided on the inner wall of the shell (101). An electric heating wire (103) is fixedly connected inside the heating groove (102). An air inlet (104) is provided on the outer surface of the shell (101). The air inlet (104) is connected to the heating groove (102). A flange (105) is fixedly connected to the outer surface of the shell (101). A threaded cap (106) is threadedly connected to the flange (105). The end of the threaded cap (106) is... A filter screen (107) is provided. The gas circulation mechanism (2) includes a top cover (201). A fixing plate (204) is fixedly connected to the inner wall of the top cover (201). A temperature detection sensor (205) is fixedly connected to the lower surface of the fixing plate (204). An oxygen concentration detection sensor (206) is fixedly connected to the middle of the lower surface of the fixing plate (204). A fan (207) is fixedly connected to the inner top of the top cover (201). An air exchange port (208) is opened on the top of the top cover (201). The position of the air exchange port (208) corresponds to the position of the fan (207).

2. The gas circulation system for a cell culture chamber according to claim 1, characterized in that: The top of the outer casing (101) is provided with a mounting groove (108), and the bottom of the upper cover (201) is located inside the mounting groove (108).

3. The gas circulation system for a cell culture chamber according to claim 2, characterized in that: A rubber ring (109) is installed inside the mounting groove (108), and the bottom of the top cover (201) is in contact with the outer surface of the rubber ring (109).

4. The gas circulation system for a cell culture chamber according to claim 1, characterized in that: A shock-absorbing rubber plate (110) is fixedly connected to the inner bottom of the outer shell (101).

5. The gas circulation system for a cell culture chamber according to claim 1, characterized in that: A power supply (202) is fixedly connected to the inner top of the cover (201), and the power supply (202) is electrically connected to the temperature detection sensor (205), the oxygen concentration detection sensor (206), and the fan (207).

6. The gas circulation system for a cell culture chamber according to claim 1, characterized in that: The upper cover (201) is internally fixedly connected with a partition net (203), which is located between the fixed plate (204) and the fan (207).

Images