Biological stem cell culture dish

Abstract

The utility model discloses a kind of biological stem cell culture dishes, including bearing shell, flip, bearing shell rear end is connected with flip by hinge;Bearing shell inside is provided with recess, the recess inside is installed with culture dish, and the flange of culture dish upper end is provided with adapter plate, and adapter plate plug-in installation is in the positioning groove inside that bearing shell recess upper end edge setting is set up.The utility model is supported in the bottom of bearing shell recess inside by the base of culture dish bottom, adapter plate is rotated in rotating groove inside, corrugated pipe is resisted in rotating groove inside, by the limiting of rotating groove, culture dish is limited installation in bearing shell inside, reduce the situation that culture dish occurs up and down deviation, avoid biological stem cell to be shaken and be destroyed;By microporous breathable membrane, oxygen and carbon dioxide are allowed to diffuse bidirectionally efficiently, while blocking microorganism and liquid water from entering the inside of culture dish, so that biological stem cell can obtain sufficient oxygen, improve the culture effect of biological stem cell.

Description

Technical Field

[0001] This utility model relates to the field of stem cell culture, and in particular to a biological stem cell culture dish. Background Technology

[0002] As a core foundation of regenerative medicine and tissue engineering, stem cell culture technology directly affects cell proliferation, differentiation, and functional maintenance through the design of its culture carriers and culture dishes. Traditional culture dishes are mainly designed for ordinary adherent cells, using materials such as polystyrene to perform hydrophilic treatment on the surface, giving them basic cell adhesion capabilities.

[0003] A prior art patent, CN222362113U, discloses a biological stem cell culture dish comprising a base, a fixing ring fixedly connected to the inner top wall of the base, and receiving columns fixedly connected to the inner walls of all four sides of the fixing ring. A limiting plate is slidably connected inside each receiving column, and a spring is installed inside each receiving column. A sliding rod is fixedly connected to one side of the limiting plate, and a clamping block is fixedly connected to one end of the sliding rod. A culture dish is disposed on the inner top wall of the base, and a cover plate is rotatably connected to the rear top of the base. A sealing ring is fixedly connected to the front end of the cover plate, and a sealing assembly is fixedly connected to the inner wall of the front end of the cover plate. This invention achieves fixed positioning of the culture dish, preventing uneven cell layer formation due to movement or vibration during culture, thus avoiding impact on the reproducibility and accuracy of experimental results.

[0004] The above-disclosed technical solution also has the following shortcomings: the culture vessel is only clamped inside the base by clamping blocks. When the base is subjected to external impact, the buffering performance between the culture vessel and the base is poor. The biological stem cells inside the culture vessel are easily shaken and damaged. In addition, the lid of the culture vessel has good sealing performance, and gas exchange mainly relies on the edge gaps. The gas exchange efficiency is low, which leads to excessive accumulation of carbon dioxide and a decrease in pH value inside the culture vessel, reducing cell viability and thus reducing the culture effect. Utility Model Content

[0005] Therefore, in order to overcome the above-mentioned shortcomings, this utility model provides a biological stem cell culture dish.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a biological stem cell culture dish, comprising a supporting shell and a flip cover, wherein the rear end of the supporting shell is connected to the flip cover via a hinge;

[0007] Preferably, the carrier shell has a groove inside, and a culture vessel is installed inside the groove. A receiving plate is provided on the upper edge of the culture vessel, and the receiving plate is inserted into the positioning groove provided on the upper edge of the groove of the carrier shell. The carrier shell also has a rotating groove inside, and the receiving plate is slidably installed inside the rotating groove. A corrugated pipe is provided at the bottom of the receiving plate, and the corrugated pipe abuts against the bottom of the inner side of the rotating groove.

[0008] In a further preferred embodiment, the bottom of the culture vessel is nested inside the sleeve, and a base is installed at the bottom of the sleeve, the base being installed at the bottom of the groove of the support shell.

[0009] In a further preferred embodiment, the support shell is further equipped with a spring rod, a sliding block, a connecting rod, and a clamping plate. The spring rod is fixed to the inner wall of the support shell, and the sliding block is slidably mounted on the spring rod. The sliding block is axially connected to one end of the connecting rod, and the other end of the connecting rod is axially connected to the middle of the outer end of the clamping plate. The clamping plate clamps the outer surface of the culture vessel.

[0010] In a further preferred embodiment, the inner wall of the culture vessel is provided with a photodegradable layer and a thermosensitive layer, with the photodegradable layer located outside the thermosensitive layer.

[0011] In a further preferred embodiment, the flip cover has a through groove in the middle, and a top cover is installed inside the through groove. The top cover is located above the culture vessel, and a sealing ring is provided in the middle of the top cover. The sealing ring is engaged and installed at the upper end of the groove formed between the inner wall of the culture vessel and the photodegradable layer and the temperature-sensitive layer.

[0012] In a further preferred embodiment, the top cover is further provided with a microporous breathable membrane, and the microporous breathable membrane is located directly above the culture vessel.

[0013] In a further preferred embodiment, the upper end of the bearing shell is further provided with a water storage tank and a connecting cavity, one end of the connecting cavity being connected to the upper edge of the water storage tank, and the other end of the connecting cavity being connected to the upper end of the groove of the bearing shell.

[0014] In a further preferred embodiment, two microporous breathable membranes are provided, with the two microporous breathable membranes respectively installed in the upper and lower layers inside the top cover.

[0015] The beneficial effects of this utility model are:

[0016] This invention uses a base at the bottom of the culture vessel to support the bottom of the groove inside the carrier shell. Then, the culture vessel is rotated, causing the receiving plate to rotate inside the rotating groove. At the same time, the corrugated tube at the bottom of the receiving plate abuts against the inside of the rotating groove. Through the limiting of the rotating groove and the elastic support of the corrugated tube, the culture vessel is fixedly installed inside the carrier shell, reducing the vertical displacement of the culture vessel and providing elastic buffering for vertical displacement. Then, four sets of clamping plates improve the stability of the culture vessel in the carrier shell, preventing the biological stem cells inside the culture vessel from shaking and being damaged.

[0017] This invention allows for efficient bidirectional diffusion of oxygen and carbon dioxide through a microporous breathable membrane, while blocking microorganisms and liquid water from entering the culture vessel. This ensures that biological stem cells receive sufficient oxygen during culture, improving cell viability and thus enhancing the culture effect of biological stem cells inside the culture vessel. Attached Figure Description

[0018] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0019] Figure 2 This is a utility model Figure 1 A magnified view of the structure at point A in the middle;

[0020] Figure 3 This is a three-dimensional structural diagram of the culture vessel of this utility model;

[0021] Figure 4 This is a top view of the internal structure of the bearing shell of this utility model, as well as a partially enlarged structural diagram;

[0022] Figure 5 This is a three-dimensional structural diagram of the top cover of this utility model;

[0023] Figure 6 This is a three-dimensional structural diagram of the flip cover of this utility model.

[0024] The components include: a support shell-1, a culture vessel-11, a photodegradable layer-1101, a temperature-sensitive layer-1102, a sleeve-1103, a base-1104, a spring rod-101, a sliding block-102, a connecting support rod-103, a clamping plate-104, a positioning groove-105, a rotating groove-106, a connecting plate-111, a corrugated pipe-1111, a water storage tank-12, a connecting cavity-121, a flip cover-2, a top cover-21, a sealing ring-22, and a microporous breathable membrane-23. Detailed Implementation

[0025] To further explain the technical solution of this utility model, a detailed description is provided below through specific embodiments.

[0026] Please see Figure 1-2 This utility model provides a biological stem cell culture dish, including a support shell 1 and a flip cover 2, wherein the rear end of the support shell 1 is connected to the flip cover 2 by a hinge;

[0027] The carrier shell 1 has a groove inside, and a culture vessel 11 is installed inside the groove. A receiving plate 111 is provided on the upper edge of the culture vessel 11, and the receiving plate 111 is inserted into the positioning groove 105 provided on the upper edge of the groove of the carrier shell 1. The carrier shell 1 also has a rotating groove 106 inside, and the receiving plate 111 is slidably installed inside the rotating groove 106. A corrugated pipe 1111 is provided at the bottom of the receiving plate 111, and the corrugated pipe 1111 abuts against the bottom of the inner side of the rotating groove 106.

[0028] It should be noted that the groove inside the carrier shell 1 is a cylindrical structure that fits the cylindrical culture vessel 11, allowing culture vessels 11 of different sizes to be placed inside the groove of the carrier shell 1.

[0029] The culture vessel 11 is provided with a receiving plate 111 at both the front and rear ends, and the support shell 1 is also provided with two positioning grooves 105 and a rotating groove 106 to cooperate with the two receiving plates 111. The receiving plates 111 are inserted into the positioning grooves 105 and slide along the rotating grooves 106. The receiving plates 111 rotate into the rotating grooves 106, reducing the vertical displacement of the culture vessel 11 inside the support shell 1.

[0030] The corrugated tube 1111 at the bottom of the receiving plate 111 is made of rubber and has good elastic cushioning performance. The corrugated tube 1111 abuts against the inside of the rotating groove 106, providing elastic cushioning for the up and down movement of the receiving plate 111 inside the rotating groove 106, thereby providing elastic cushioning for the up and down movement of the culture vessel 11.

[0031] Please see Figure 3 This utility model provides a biological stem cell culture dish. The bottom of the culture dish 11 is nested inside the sleeve 1103, and a base 1104 is installed at the bottom of the sleeve 1103. The base 1104 is installed at the bottom of the groove of the support shell 1. A photodegradable layer 1101 and a temperature-sensitive layer 1102 are provided on the inner wall of the culture dish 11, and the photodegradable layer 1101 is located outside the temperature-sensitive layer 1102.

[0032] It should be noted that both the sleeve 1103 and the base 1104 are made of rubber, which has good resilience. The base 1104 is installed inside the sleeve 1103 by nesting the lower end of the culture vessel 11, so that the culture vessel 11 provides elastic support for the culture vessel 11 inside the support shell 1, and avoids the biological stem cells inside the culture vessel 11 from shaking and being damaged.

[0033] The photodegradable layer 1101 is made of Nvoc-peptide material (o-nitroveratroloxycarbonyl-peptide), which is a high-precision photoresponsive biomaterial. By irradiating a UV lamp locally, Nvoc breaks down into RGD and detaches, thereby achieving the separation of target cells. It can promote cell proliferation and maintain function, thereby improving the culture effect of biological stem cells in the culture vessel 11.

[0034] The temperature-sensitive layer 1102 uses pNIPAM (poly-N-isopropylacrylamide), a classic temperature-responsive smart polymer material. During cultivation, the temperature around the culture vessel 11 should be controlled at 37℃. At this temperature, the isopropyl hydrophobicity of pNIPAM is enhanced, hydrogen bonds break, molecular chains dehydrate and curl up into clusters, and the surface abruptly becomes hydrophobic, which is conducive to the attachment and growth of biological stem cells and improves the cultivation effect. After cultivation, when it is necessary to detach the cells from the inner wall of the culture vessel 11, the temperature around the culture vessel 11 should be controlled at less than 32℃. At this temperature, the amide groups of pNIPAM form hydrogen bonds with water molecules, the molecular chains are highly hydrated and extended, and the surface becomes hydrophilic, which is conducive to the detachment of biological stem cells after cultivation.

[0035] Please see Figure 4 This utility model provides a biological stem cell culture dish. The support shell 1 is also equipped with a spring rod 101, a sliding block 102, a connecting rod 103, and a clamping plate 104. The spring rod 101 is fixed to the inner wall of the support shell 1, and the sliding block 102 is slidably mounted on the spring rod 101. The sliding block 102 is axially connected to one end of the connecting rod 103, and the other end of the connecting rod 103 is axially connected to the middle part of the outer side of the clamping plate 104. The clamping plate 104 clamps the outer surface of the culture dish 11.

[0036] The spring rod 101 consists of a guide rod and a spring, and two sliding blocks 102 are installed on the spring rod 101. When the spring rod 101 provides reverse elastic guidance for the two sliding blocks 102, the two linkage support rods 103 work together to make the clamping plate 104 elastically clamp the outer surface of the culture vessel 11. The stability of the culture vessel 11 clamped and fixed inside the support shell 1 is improved by the four sets of clamping plates 104.

[0037] In this embodiment, the culture vessel 11 is placed inside the carrier shell 1. The two connecting plates 111 at the upper end of the culture vessel 11 are aligned with the two positioning slots 105 and inserted. At this time, the base 1104 at the bottom of the culture vessel 11 is supported on the bottom of the groove inside the carrier shell 1. Then, the culture vessel 11 is rotated, so that the connecting plates 111 rotate inside the rotating slot 106. At the same time, the corrugated tube 1111 at the bottom of the connecting plate 111 abuts against the inside of the rotating slot 106. Through the limiting of the rotating slot 106 and the elastic support of the corrugated tube 1111, the culture vessel 11 is fixedly installed inside the carrier shell 1, reducing the vertical displacement of the culture vessel 11 and providing elastic buffering for vertical displacement of the culture vessel 11. Then, the stability of the culture vessel 11 being clamped and fixed inside the carrier shell 1 is improved by the four sets of clamping plates 104, so as to prevent the biological stem cells inside the culture vessel 11 from shaking and being damaged.

[0038] Please see Figure 1 , 5 6. This utility model provides a biological stem cell culture dish, with a through groove in the middle of the flip cover 2, and a top cover 21 installed inside the through groove. The top cover 21 is located above the culture dish 11, and a sealing ring 22 is provided in the middle of the top cover 21. The sealing ring 22 is engaged and installed at the upper end of the groove formed between the inner wall of the culture dish 11 and the photodegradable layer 1101 and the temperature-sensitive layer 1102.

[0039] The top cover 21 is also provided with a microporous breathable membrane 23, and the microporous breathable membrane 23 is located directly above the culture vessel 11.

[0040] Two microporous breathable membranes 23 are provided, and the two microporous breathable membranes 23 are respectively installed in the upper and lower layers inside the top cover 21;

[0041] It should be noted that the sealing ring 22 has a circular structure, and the outer diameter of the sealing ring 22 is consistent with the inner diameter of the culture vessel 11. When the flip cover 2 is closed at the upper end of the support shell 1, the flip cover 2 flips down and the sealing ring 22 is inserted into the culture vessel 11, which improves the sealing effect of the edge of the culture vessel 11 and prevents biological stem cells from leaking out from the edge of the culture vessel 11.

[0042] The microporous breathable membrane 23 is made of hydrophobic PTFE material with a pore size of about 0.1-0.5 micrometers. It allows for efficient bidirectional diffusion of oxygen and carbon dioxide while blocking microorganisms (bacteria, fungi, spores) and liquid water. Gas exchange can be achieved inside the culture dish 11 without opening the top cover 21, effectively avoiding the situation of excessive oxygen consumption, excessive carbon dioxide accumulation, and pH drop inside the culture dish 11. By improving cell viability through gas exchange, the culture effect is improved.

[0043] The upper end of the bearing shell 1 is also provided with a water storage tank 12 and a connecting cavity 121. One end of the connecting cavity 121 is connected to the upper edge of the water storage tank 12, and the other end of the connecting cavity 121 is connected to the upper end of the groove of the bearing shell 1.

[0044] It should be noted that there are four water storage tanks 12 and four connecting cavities 121. Sterile water is added inside the water storage tanks 12 through the four water storage tanks 12 and connecting cavities 121, which avoids direct contact between the sterile water and the culture vessel 11. At the same time, it can improve the humidity inside the support shell 1 after the flip cover 2 is closed at the top of the support shell 1, and reduce the drying speed of the culture medium inside the culture vessel 11.

[0045] In this embodiment, when the biological stem cells are cultured inside the culture vessel 11, the flip cover 2 is closed at the top of the support shell 1. The flip cover 2 causes the top cover 21 to flip down, at which point the sealing ring 22 is inserted into the culture vessel 11. The microporous breathable membrane 23 is positioned directly above the culture vessel 11. The microporous breathable membrane 23 allows for efficient bidirectional diffusion of oxygen and carbon dioxide, while blocking microorganisms and liquid water from entering the culture vessel 11. This ensures that the biological stem cells can obtain sufficient oxygen when cultured inside the culture vessel 11, thereby improving cell viability and enhancing the culture effect of the biological stem cells inside the culture vessel 11.

[0046] The control method of this utility model is to control the device by manually starting and stopping the switch. The wiring diagram of the power element and the supply of power are common knowledge in the field. Since this utility model is mainly used to protect mechanical devices, the control method and wiring layout will not be explained in detail.

[0047] The control method of this utility model is automatic control through a controller. The control circuit of the controller can be implemented by simple programming by those skilled in the art. The power supply is also common knowledge in the field. Since this utility model is mainly used to protect mechanical devices, the control method and circuit connection will not be explained in detail.

[0048] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A biological stem cell culture dish, comprising a support shell (1) and a flip cover (2), wherein the rear end of the support shell (1) is connected to the flip cover (2) via a hinge; Its features are: The support shell (1) has a groove inside, and a culture vessel (11) is installed inside the groove. A receiving plate (111) is provided on the upper edge of the culture vessel (11), and the receiving plate (111) is inserted into the positioning groove (105) provided on the upper edge of the groove of the support shell (1). The support shell (1) also has a rotating groove (106) inside, and the receiving plate (111) is slidably installed inside the rotating groove (106). A corrugated pipe (1111) is provided at the bottom of the receiving plate (111), and the corrugated pipe (1111) abuts against the bottom of the inner side of the rotating groove (106).

2. The biological stem cell culture dish according to claim 1, characterized in that: The bottom of the culture vessel (11) is nested inside the sleeve (1103), and a base (1104) is installed at the bottom of the sleeve (1103), the base (1104) being installed at the bottom of the groove of the support shell (1).

3. The biological stem cell culture dish according to claim 2, characterized in that: The support shell (1) is also equipped with a spring rod (101), a sliding block (102), a connecting rod (103), and a clamping plate (104). The spring rod (101) is fixed to the inner wall of the support shell (1), and the sliding block (102) is slidably mounted on the spring rod (101). The sliding block (102) is axially connected to one end of the connecting rod (103), and the other end of the connecting rod (103) is axially connected to the middle part of the outer side of the clamping plate (104). The clamping plate (104) clamps the outer surface of the culture vessel (11).

4. The biological stem cell culture dish according to claim 3, characterized in that: The culture vessel (11) has a photodegradable layer (1101) and a thermosensitive layer (1102) on its inner wall, and the photodegradable layer (1101) is located outside the thermosensitive layer (1102).

5. The biological stem cell culture dish according to claim 1, characterized in that: The flip cover (2) has a through groove in the middle, and a top cover (21) is installed inside the through groove. The top cover (21) is located above the culture vessel (11). A sealing ring (22) is provided in the middle of the top cover (21), and the sealing ring (22) is engaged and installed at the upper end of the groove formed between the inner wall of the culture vessel (11) and the photodegradation layer (1101) and the temperature-sensitive layer (1102).

6. The biological stem cell culture dish according to claim 5, characterized in that: The top cover (21) is also provided with a microporous breathable membrane (23), and the microporous breathable membrane (23) is located directly above the culture vessel (11).

7. The biological stem cell culture dish according to claim 1, characterized in that: The upper end of the bearing shell (1) is also provided with a water storage tank (12) and a connecting cavity (121). One end of the connecting cavity (121) is connected to the upper edge of the water storage tank (12), and the other end of the connecting cavity (121) is connected to the upper end of the groove of the bearing shell (1).

8. The biological stem cell culture dish according to claim 6, characterized in that: Two microporous breathable membranes (23) are provided, and the two microporous breathable membranes (23) are respectively installed in the upper and lower layers inside the top cover (21).

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