An improved cell culture workstation

Abstract

The utility model relates to material containing mechanism technical field, especially disclose an improved cell culture workstation, including material containing mechanism, with the liquid pump of material containing mechanism cooperation and set up between material containing mechanism and liquid pump's communication pipeline, material containing mechanism includes material containing box, sets up the moving mechanism on material containing box and sets up the material containing frame on moving mechanism, and the external container spare of material containing frame bears for containing cell culture solution, and the container spare carried by material containing frame moves in and out material containing box through moving mechanism, the container spare carried by material containing frame communicates with communication pipeline, and first valve body is equipped on communication pipeline, and first valve body is used for controlling the on-off state of communication pipeline, according to the actual demand of cell culture flexible regulation liquid transmission's start -stop, has promoted the convenience of operation and the stability of culture process.

Description

Technical Field

[0001] This utility model relates to the field of material holding mechanism technology, and in particular discloses an improved cell culture workstation. Background Technology

[0002] Cell culture demands extremely high standards for sterility, precision in fluid transfer, and ease of operation. However, existing cell culture workstations generally suffer from several shortcomings. For instance, the connection between the on / off control of the tubing used to deliver cell culture medium and the emptying function of the tubing is poor, often resulting in liquid residue or incomplete aspiration due to pressure imbalance within the tubing. Furthermore, the introduction of external air into the tubing can easily introduce the risk of microbial contamination. Additionally, the installation and replacement of containers are cumbersome, lacking convenient moving guide structures, and the storage of tubing within the container is often disorganized. Therefore, there is an urgent need for a cell culture workstation that optimizes and improves tubing control, sterility protection, ease of operation, and disinfection to meet the practical needs of high-quality cell culture. Utility Model Content

[0003] In order to overcome the shortcomings and deficiencies of the existing technology, the purpose of this utility model is to provide an improved cell culture workstation.

[0004] To achieve the above objectives, this utility model provides an improved cell culture workstation, comprising a material holding mechanism, a pump used in conjunction with the material holding mechanism, and a connecting pipe disposed between the material holding mechanism and the pump. The material holding mechanism includes a material tank, a moving mechanism disposed on the material tank, and a material rack disposed on the moving mechanism. The material rack carries an external container for holding cell culture medium, and the container carried by the material rack moves in and out of the material tank via the moving mechanism. The container carried by the material rack is connected to the connecting pipe, and a first valve body is provided on the connecting pipe for controlling the on / off state of the connecting pipe. When the first valve body is in the closed state, the connecting pipe is in a conductive state, allowing the liquid in the material holding mechanism to flow along the connecting pipe towards the pump. When the first valve body is in the open state, the connecting pipe is in a closed state, and the flow of liquid in the material holding mechanism is blocked.

[0005] The core structure includes a container, a pump, and a connecting pipe. A first valve is mounted on the connecting pipe to control its flow. When the first valve is closed, the connecting pipe is open, allowing liquid in the container to flow to the pump. When the first valve is opened, the connecting pipe is closed, stopping the flow of liquid. The advantage of this structure is that the first valve enables precise and rapid control of the cell culture medium transfer process, avoiding the lag problem of traditional pipe control methods. Furthermore, the mechanical on / off control reduces direct contact between the valve and the culture medium, lowering the risk of contamination. It also allows for flexible adjustment of the liquid transfer process according to the actual needs of cell culture, improving operational convenience and the stability of the culture process. The external container can be a bottle or a container bag. When the external container is a container bag, a hook is provided on the container rack for holding the bag.

[0006] The core structure includes a material holding mechanism, a liquid pump, and a connecting pipe between the two. A first valve body is installed on the connecting pipe to control the flow of the liquid. When the first valve body is closed, the connecting pipe is open, and the liquid in the material holding mechanism can flow along the pipe to the liquid pump. When the first valve body is switched to the open state, the connecting pipe is closed, and the liquid flow is blocked. The beneficial effect of this structure is that it enables precise and rapid control of the cell culture medium transfer process through the first valve body, avoiding the lag problem of traditional pipeline control methods. At the same time, the mechanical opening and closing control reduces the direct contact between the valve and the culture medium, reducing the risk of contamination. Furthermore, it can flexibly adjust the start and stop of liquid transfer according to the actual needs of cell culture, improving the convenience of operation and the stability of the culture process.

[0007] The first valve body divides the connecting pipeline into a first pipeline and a second pipeline connected to the first pipeline. The first pipeline is located between the first valve body and the material receiving mechanism, and the second pipeline is located between the first valve body and the liquid pump.

[0008] The culture workstation also includes a third pipe connected to the second pipe, and a second valve body is provided on the third pipe. When the first valve body is in the open state and the second valve body is in the closed state, air flows into the second pipe through the third pipe to balance the air pressure inside the pipe, so that the cell culture medium stored in the second pipe can be emptied by the suction action of the pump.

[0009] The first valve body divides the connecting pipeline into a first pipeline located between it and the material holding mechanism and a second pipeline located between it and the pump. A third pipeline connected to the second pipeline and equipped with a second valve body is also provided. In practice, with the first valve body closed, the connecting pipeline is open to allow liquid in the material holding mechanism to flow to the pump. With the first valve body open and the second valve body closed, air can flow into the second pipeline through the third pipeline to balance the air pressure, which, in conjunction with the pump, empties the cell culture medium from the second pipeline. This design not only achieves precise and controllable liquid flow between the material holding mechanism and the pump but also effectively avoids residual cell culture medium in the second pipeline, improving the thoroughness of liquid transfer, facilitating operation, and further ensuring the stability and reliability of the cell culture process.

[0010] The air inlet of the third pipe is equipped with a sterile filter. After external air is filtered by the sterile filter to remove microorganisms, it flows into the second pipe through the third pipe to balance the air pressure inside the pipe.

[0011] With the first valve closed, the connecting pipe is open to allow liquid in the container to flow to the pump. With the first valve open and the second valve closed, external air, after being filtered by a sterile filter to remove microorganisms, flows into the second pipe through the third pipe to balance the air pressure. This, combined with the pump, empties the cell culture medium in the second pipe. This design not only achieves precise and controllable liquid flow between the container and the pump, effectively avoiding residual cell culture medium in the second pipe and improving the thoroughness of liquid transfer, but also prevents external microorganisms from contaminating the cell culture medium, ensuring the sterility of the cell culture environment. It is easy to operate and further improves the stability and reliability of the cell culture process.

[0012] The moving mechanism includes a moving guide rail and a sliding block that slides with the moving guide rail. The container rack includes a placement rack connected to the sliding block and a container placed on the placement rack. The moving guide rail is provided with a sliding groove, and the sliding block is housed in the sliding groove. When it is necessary to replace the container, the placement rack is pulled to drive the sliding block to slide outward along the extension direction of the sliding groove, so that the placement rack and the container are partially or completely removed from the container. After the replacement is completed, the placement rack is pushed in the opposite direction to move the new container into the preset position in the container.

[0013] The container rack is moved and positioned inside the container box via a moving mechanism. When changing containers, pulling the rack causes the sliding block to slide along the sliding groove to the outside of the container box, which can partially or completely remove the rack along with the container. After replacement, pushing it in the opposite direction moves it into the preset position. The sliding moving structure makes the container replacement operation convenient and efficient, significantly improving the overall operational flexibility and maintenance convenience, and further enhancing the stability and reliability of the cell culture process.

[0014] The material holding mechanism also includes a flexible pipe storage rack located inside the material holding tank, through which the connecting pipes are stored and secured.

[0015] Pipe organizers allow for the neat and secure storage of connecting pipes, preventing them from becoming tangled and affecting operation or causing contamination. This significantly improves the overall cleanliness, operational flexibility, and ease of maintenance of the equipment.

[0016] The material holding mechanism also includes a sealing door that can be opened and closed and is installed on the material holding box. The edge of the sealing door near the material holding box is provided with a magnetic element, and the material holding box is provided with a matching magnetic attraction part corresponding to the position of the magnetic element.

[0017] The magnetic components and magnetic attraction mating parts can quickly open and close the sealing door and seal the container. This design is convenient to operate and has high sealing reliability. It can effectively isolate external contamination, provide a stable sterile environment for cell culture, and simplify the opening and closing of the container, thus improving the ease of use in the cell culture process.

[0018] The pump is a peristaltic pump. The culture workstation also includes a control center set on the container tank. The pump is electrically connected to the control center. The pump includes a housing, a drive unit set on the housing, and a pump head. The pump head is provided with a rotating shaft connected to the output end of the drive unit. The control center controls the rotation of the rotating shaft on the pump head to cooperate with the housing to squeeze the connecting pipe and control the transfer volume of the cell culture medium.

[0019] The pump is a peristaltic pump electrically connected to the control center. The peristaltic pump includes a housing, a drive unit mounted on the housing, and a pump head. The pump head has a rotating shaft connected to the output end of the drive unit. In practice, the control center controls the rotation of the pump head's rotating shaft. The rotating shaft and the housing work together to squeeze and connect the pipes, thereby precisely controlling the transfer volume of the cell culture medium. This design, through the combination of electrical control and a peristaltic pump, achieves automated and precise control of the culture medium transfer. It is easy to operate, and the squeezing transfer method of the peristaltic pump reduces contamination of the culture medium. At the same time, the compact and reasonable layout of the control center integrated into the container further improves the convenience of equipment use and the stability of cell culture.

[0020] The material holding mechanism also includes a disinfection mechanism installed inside the material holding tank or on the sealing door. When the sealing door is closed, the disinfection mechanism disinfects the inside of the material holding tank.

[0021] The disinfection mechanism is an ultraviolet disinfection component. When the sealed door is closed, the disinfection mechanism is automatically activated to disinfect the internal space of the container. This design triggers the disinfection operation through the linkage of the sealed door closing, without the need for additional manual control. It is convenient to operate and can promptly remove microorganisms in the container, further enhancing the sterility of the cell culture environment, providing a more reliable environmental guarantee for cell culture, and improving the success rate and stability of cell culture.

[0022] The first valve body and / or the second valve body are located inside the material container, or the first valve body and / or the second valve body are located outside the material container.

[0023] The improved cell culture workstation also includes a cooling unit for cooling the contents of the container, which is used to refrigerate and store the cell culture medium contained in the container.

[0024] The beneficial effects of this invention are as follows: By integrating the material handling mechanism, peristaltic pump, connecting pipeline, and control center, the system uses a first valve to control the flow of liquid, a second valve and a third pipeline with a sterile filter to balance air pressure and remove pipeline residues, and a control center to regulate the peristaltic pump for precise transfer of culture medium. Combined with a sliding tank movement structure, pipeline storage rack, magnetic sealing door, and a UV disinfection mechanism linked to door closure, an integrated sterile cell culture system is constructed. Its advantages lie in achieving automated and precise control of culture medium transfer, effectively avoiding liquid residue and external microbial contamination, simplifying the operation process of container replacement, tank opening and closing, and pipeline organization, ensuring the continuous sterility and stability of the cell culture environment, improving the overall cleanliness and ease of maintenance of the equipment, and significantly increasing the success rate and operational reliability of cell culture. Attached Figure Description

[0025] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0026] Figure 2 For the present utility model Figure 1 A magnified structural diagram of part A in the middle;

[0027] Figure 3 This is an exploded view of the entire utility model;

[0028] Figure 4 This is a cross-sectional view of the entire utility model;

[0029] Figure 5 This is a schematic diagram of the liquid pump of this utility model.

[0030] The reference numerals in the figures include:

[0031] 1. Material holding mechanism; 2. Liquid pump; 3. Connecting pipe; 4. First valve body; 5. First pipe; 6. Second pipe; 7. Third pipe; 8. Second valve body; 9. Sterile filter; 11. Material tank; 12. Moving guide rail; 13. Placement rack; 14. Sliding block; 15. Container component; 16. Sliding groove; 17. Pipe storage rack; 18. Sealing door; 19. Magnetic component; 21. Magnetic attraction mating part; 22. Control center; 23. Housing; 24. Drive component; 25. Pump head; 27. Sterilization mechanism. Detailed Implementation

[0032] To facilitate understanding by those skilled in the art, the present invention will be further described below with reference to embodiments and accompanying drawings. The content mentioned in the embodiments is not intended to limit the present invention.

[0033] Please see Figures 1 to 5 As shown, an improved cell culture workstation of this utility model includes a material holding mechanism 1, a liquid pump 2 used in conjunction with the material holding mechanism 1, and a connecting pipe 3 disposed between the material holding mechanism 1 and the liquid pump 2. The material holding mechanism 1 includes a material tank 11, a moving mechanism disposed on the material tank 11, and a material rack disposed on the moving mechanism. The material rack carries an external container 15 for holding cell culture medium. The container 15 carried by the material rack moves in and out of the material tank 11 via the moving mechanism. The container 15 carried by the material rack is connected to the connecting pipe 3. A first valve body 4 is provided on the connecting pipe 3. The first valve body 4 is used to control the on / off state of the connecting pipe 3. When the first valve body 4 is in the closed state, the connecting pipe 3 is in the conducting state, allowing the liquid in the material holding mechanism 1 to flow along the connecting pipe 3 towards the liquid pump 2. When the first valve body 4 is in the open state, the connecting pipe 3 is in the closed state, and the flow of liquid in the material holding mechanism 1 is blocked.

[0034] The core structure includes a material holding mechanism 1, a liquid pump 2, and a connecting pipe 3 connecting the two. A first valve body 4 for controlling the opening and closing of the pipe is installed on the connecting pipe 3. When the first valve body 4 is in the closed state, the connecting pipe 3 is open, and the liquid in the material holding mechanism 1 can flow along the pipe to the liquid pump 2. When the first valve body 4 is switched to the open state, the connecting pipe 3 is closed, and the liquid flow is blocked. The beneficial effect of this structure is that it enables precise and rapid control of the cell culture medium transfer process through the first valve body 4, avoiding the problem of lag in response of traditional pipe control methods. At the same time, the mechanical opening and closing control reduces the direct contact between the valve and the culture medium, reducing the risk of contamination. Furthermore, it can flexibly adjust the start and stop of liquid transfer according to the actual needs of cell culture, improving the convenience of operation and the stability of the culture process.

[0035] The core structure includes a material holding mechanism 1, a liquid pump 2, and a connecting pipe 3 connecting the two. A first valve body 4 for controlling the opening and closing of the pipe is installed on the connecting pipe 3. When the first valve body 4 is in the closed state, the connecting pipe 3 is open, and the liquid in the material holding mechanism 1 can flow along the pipe to the liquid pump 2. When the first valve body 4 is switched to the open state, the connecting pipe 3 is closed, and the liquid flow is blocked. The beneficial effect of this structure is that it enables precise and rapid control of the cell culture medium transfer process through the first valve body 4, avoiding the problem of lag in response of traditional pipe control methods. At the same time, the mechanical opening and closing control reduces the direct contact between the valve and the culture medium, reducing the risk of contamination. Furthermore, it can flexibly adjust the start and stop of liquid transfer according to the actual needs of cell culture, improving the convenience of operation and the stability of the culture process.

[0036] The first valve body 4 divides the connecting pipe 3 into a first pipe 5 and a second pipe 6 connected to the first pipe 5. The first pipe 5 is located between the first valve body 4 and the material receiving mechanism 1, and the second pipe 6 is located between the first valve body 4 and the liquid pump 2.

[0037] The culture workstation also includes a third pipe 7 connected to the second pipe 6, and a second valve body 8 is provided on the third pipe 7. When the first valve body 4 is in the open state and the second valve body 8 is in the closed state, air flows into the second pipe 6 through the third pipe 7 to balance the air pressure in the pipe, so that the cell culture medium stored in the second pipe 6 can be emptied by the suction action of the pump 2.

[0038] The first valve body 4 divides the connecting pipe 3 into a first pipe 5 located between it and the material holding mechanism 1 and a second pipe 6 located between it and the pump 2. At the same time, a third pipe 7 connected to the second pipe 6 and equipped with a second valve body 8 is configured. In specific implementation, when the first valve body 4 is closed, the connecting pipe 3 is open to allow the liquid in the material holding mechanism 1 to flow to the pump 2. When the first valve body 4 is open and the second valve body 8 is closed, air can flow into the second pipe 6 through the third pipe 7 to balance the air pressure in the pipe. This works in conjunction with the pump 2 to aspirate and empty the cell culture medium in the second pipe 6. This design not only achieves precise and controllable liquid flow between the material holding mechanism 1 and the pump 2, but also effectively avoids cell culture medium residue in the second pipe 6, improves the thoroughness of liquid transfer, is easy to operate, and further ensures the stability and reliability of the cell culture process.

[0039] The air inlet of the third pipe 7 is equipped with a sterile filter 9. After external air is filtered by the sterile filter 9 to remove microorganisms, it flows into the second pipe 6 through the third pipe 7 to balance the air pressure inside the pipe.

[0040] With the first valve body 4 closed, the connecting pipe 3 is open to allow the liquid in the container 1 to flow to the pump 2. With the first valve body 4 open and the second valve body 8 closed, external air is filtered by the sterile filter 9 to remove microorganisms and then flows into the second pipe 6 through the third pipe 7 to balance the air pressure inside the pipe. This works in conjunction with the pump 2 to aspirate and empty the cell culture medium in the second pipe 6. This design not only achieves precise and controllable liquid flow between the container 1 and the pump 2, effectively avoiding cell culture medium residue in the second pipe 6 and improving the thoroughness of liquid transfer, but also prevents external microorganisms from contaminating the cell culture medium, ensuring the sterility of the cell culture environment. It is easy to operate and further improves the stability and reliability of the cell culture process.

[0041] The moving mechanism includes a moving guide rail 12 and a sliding block 14 that slides with the moving guide rail 12. The container rack includes a placement rack 13 connected to the sliding block 14 and a container 15 placed on the placement rack 13. The moving guide rail 12 is provided with a sliding groove 16, and the sliding block 14 is accommodated in the sliding groove 16. When it is necessary to replace the container 15, the placement rack 13 is pulled to drive the sliding block 14 to slide outward along the extension direction of the sliding groove 16, so that the placement rack 13 and the container 15 are partially or completely removed from the container 11. After the replacement is completed, the placement rack 13 is pushed in the opposite direction to move the new container 15 into the preset position in the container 11.

[0042] The container rack is moved and positioned inside the container box 11 via a moving mechanism. When replacing the container 15, the placement rack 13 is pulled to drive the sliding block 14 to slide along the sliding groove 16 to the outside of the container box 11, which can partially or completely remove the placement rack 13 along with the container 15. After replacement, it can be pushed in the opposite direction to move into the preset position. The sliding moving structure makes the replacement of the container 15 convenient and efficient, significantly improving the overall operational flexibility and maintenance convenience, and further enhancing the stability and reliability of the cell culture process.

[0043] The material holding mechanism 1 also includes a flexible pipe storage rack 17 disposed inside the material holding box 11, through which the connecting pipe 3 is stored and fixed.

[0044] The pipe storage rack 17 enables the neat and secure storage of the connecting pipes 3, preventing them from becoming tangled and affecting operation or causing pollution. This significantly improves the overall cleanliness, operational flexibility, and ease of maintenance of the equipment.

[0045] The material holding mechanism 1 also includes a sealing door 18 that can be opened and closed and is provided on the material holding box 11. The edge of the sealing door 18 near the material holding box 11 is provided with a magnetic element 19, and the material holding box 11 is provided with a matching magnetic attraction part 21 corresponding to the position of the magnetic element 19.

[0046] Through the adsorption effect of the magnetic component 19 and the magnetic attraction part 21, the opening and closing of the sealing door 18 and the sealing of the container 11 can be realized quickly. This design is convenient to operate and has high sealing reliability. It can effectively isolate external pollution and provide a stable sterile environment for cell culture. At the same time, it simplifies the opening and closing operation of the container 11 and improves the ease of use in the cell culture process.

[0047] The pump 2 is a peristaltic pump. The culture workstation also includes a control center 22 installed on the container 11. The pump 2 is electrically connected to the control center 22. The pump 2 includes a housing 23, a drive component 24 installed on the housing 23, and a pump head 25. The pump head 25 is provided with a rotating shaft connected to the output end of the drive component 24. The control center 22 controls the rotation of the rotating shaft on the pump head 25 to cooperate with the housing 23 to squeeze the connecting pipe 3 to control the transfer volume of the cell culture medium.

[0048] The pump 2 is a peristaltic pump and is electrically connected to the control center 22. The peristaltic pump includes a housing 23, a drive component 24 mounted on the housing 23, and a pump head 25. The pump head 25 is equipped with a rotating shaft connected to the output end of the drive component 24. In practice, the control center 22 controls the rotation of the rotating shaft of the pump head 25. The rotating shaft and the housing 23 cooperate to squeeze and connect the pipe 3, thereby precisely controlling the transfer volume of the cell culture medium. This design, by combining electrical control and a peristaltic pump, achieves automated and precise control of the culture medium transfer. It is easy to operate, and the squeezing transfer method of the peristaltic pump can reduce the contamination of the culture medium. At the same time, the compact and reasonable layout of the control center 22 integrated into the container 11 further improves the convenience of equipment use and the stability of cell culture.

[0049] The material holding mechanism 1 also includes a disinfection mechanism 27 installed inside the material holding box 11 or on the sealing door 18. When the sealing door 18 is closed, the disinfection mechanism 27 disinfects the inside of the material holding box 11.

[0050] The disinfection mechanism 27 is an ultraviolet disinfection component. When the sealed door 18 is closed, the disinfection mechanism 27 is automatically activated to disinfect the internal space of the container 11. This design triggers the disinfection operation through the linkage of the closing of the sealed door 18, without the need for additional manual control. It is convenient to operate and can promptly remove microorganisms in the container 11, further enhancing the sterility of the cell culture environment, providing a more reliable environmental guarantee for cell culture, and improving the success rate and stability of cell culture.

[0051] The first valve body 4 and / or the second valve body 8 are located inside the material container 11, or the first valve body 4 and / or the second valve body 8 are located outside the material container 11.

[0052] The improved cell culture workstation also includes a cooling component for cooling the contents of the container 11, which is used to refrigerate and store the cell culture medium contained in the container 15 within the container 11.

[0053] The above description is only a preferred embodiment of this utility model. For those skilled in the art, there will be changes in the specific implementation method and application scope based on the idea of ​​this utility model. The content of this specification should not be construed as a limitation of this utility model.

Claims

1. An improved cell culture workstation, comprising a container (1) for storing cell culture medium, a pump (2) used in conjunction with the container (1), and a connecting pipe (3) disposed between the container (1) and the pump (2); characterized in that: The container mechanism (1) includes a container (11), a moving mechanism disposed on the container (11), and a container rack disposed on the moving mechanism. The container rack carries external containers for containing cell culture medium. The containers carried by the container rack move in and out of the container (11) via the moving mechanism. The containers carried by the container rack are connected to a connecting pipe (3). A first valve body (4) is provided on the connecting pipe (3). The first valve body (4) is used to control the on / off state of the connecting pipe (3). When the first valve body (4) is closed, the connecting pipe (3) is in a conductive state, allowing the liquid in the container mechanism (1) to flow along the connecting pipe (3) toward the pump (2). When the first valve body (4) is open, the connecting pipe (3) is closed, and the liquid in the container mechanism (1) is blocked from flowing.

2. The improved cell culture workstation according to claim 1, characterized in that: The first valve body (4) divides the connecting pipe (3) into a first pipe (5) and a second pipe (6) connected to the first pipe (5). The first pipe (5) is located between the first valve body (4) and the material holding mechanism (1), and the second pipe (6) is located between the first valve body (4) and the liquid pump (2).

3. An improved cell culture workstation according to claim 2, characterized in that: The culture workstation also includes a third pipe (7) connected to the second pipe (6), and a second valve body (8) is provided on the third pipe (7); when the first valve body (4) is in the open state and the second valve body (8) is in the closed state, air flows into the second pipe (6) through the third pipe (7) to balance the air pressure in the pipe, so that the cell culture medium stored in the second pipe (6) can be emptied by the suction action of the pump (2).

4. An improved cell culture workstation according to claim 3, characterized in that: The air inlet of the third pipe (7) is equipped with a sterile filter (9). After the external air is filtered by the sterile filter (9) to remove microorganisms, it flows into the second pipe (6) through the third pipe (7) to balance the air pressure inside the pipe.

5. An improved cell culture workstation according to claim 1, characterized in that: The moving mechanism includes a moving guide rail (12) and a sliding block (14) that slides with the moving guide rail (12). The container rack includes a placement rack (13) connected to the sliding block (14) and a container (15) placed on the placement rack (13). The moving guide rail (12) is provided with a sliding groove (16), and the sliding block (14) is housed in the sliding groove (16). When it is necessary to replace the container (15), the placement rack (13) is pulled to drive the sliding block (14) to slide along the extension direction of the sliding groove (16) to the outside of the container (11), so that the placement rack (13) and the container (15) are partially or completely removed from the container (11). After the replacement is completed, the placement rack (13) is pushed in the opposite direction to move the new container (15) into the preset position in the container (11).

6. An improved cell culture workstation according to claim 1, characterized in that: The material holding mechanism (1) also includes a flexible pipe storage rack (17) disposed inside the material holding box (11), through which the connecting pipe (3) is stored and fixed.

7. An improved cell culture workstation according to claim 1, characterized in that: The material holding mechanism (1) also includes a sealing door (18) that can be opened and closed and is provided on the material holding box (11). The sealing door (18) has a magnetic element (19) on the edge near the material holding box (11). The material holding box (11) has a matching magnetic attraction part (21) corresponding to the position of the magnetic element (19).

8. An improved cell culture workstation according to claim 1, characterized in that: The pump (2) is a peristaltic pump. The culture workstation also includes a control center (22) set on the container (11). The pump (2) is electrically connected to the control center (22). The pump (2) includes a housing (23), a drive unit (24) set on the housing (23), and a pump head (25). The pump head (25) is provided with a rotating shaft connected to the output end of the drive unit (24). The control center (22) controls the rotation of the rotating shaft on the pump head (25) to cooperate with the housing (23) to squeeze the connecting pipe (3) to control the transfer volume of the cell culture medium.

9. An improved cell culture workstation according to claim 7, characterized in that: The material holding mechanism (1) also includes a disinfection mechanism (27) installed inside the material holding box (11) or on the sealing door (18). When the sealing door (18) is closed, the disinfection mechanism (27) disinfects the inside of the material holding box (11).

10. An improved cell culture workstation according to claim 3, characterized in that: The first valve body (4) and / or the second valve body (8) are located inside the container (11), or the first valve body (4) and / or the second valve body (8) are located outside the container (11).

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