A car-t cell expansion flask rocker

Abstract

The utility model discloses a CAR T cell amplification culture bottle shaking rack relates to culture bottle shaking rack field, the utility model discloses a shaking rack main part, the upper of shaking rack main part is connected with the placement frame through the wobble mechanism, the upper of placement frame is through the limiting mechanism to the abutment spacing of several groups culture bottles, the limiting mechanism includes four groups of inserting sleeve, four groups inserting sleeve are fixedly arranged in the upper four corners of placement frame, the inside of inserting sleeve is adapted and inserts and has the inserting rod, the utility model discloses a limiting mechanism, when needing to replace the next batch culture bottle and carry out the wobble operation, only need to lift the limiting mechanism, make the inserting rod and inserting sleeve separate, then place culture bottle on the placement frame, after reinserting rod and inserting sleeve are inserted, recomplete the abutment spacing of new culture bottle, to this instead of traditional frequent regulation spacing abutment rod's operation, promote the efficiency and convenience of replacing the next batch culture bottle.

Description

Technical Field

[0001] This utility model relates to the field of culture flask shakers, specifically a CAR-T cell expansion culture flask shaker. Background Technology

[0002] In vitro expansion of CAR-T cells is a key step in the production process. Currently, the mainstream expansion equipment mainly includes wave-type bioreactors, static culture systems, and integrated fully automated equipment. Among them, the culture flask shaker, also known as a shake bottle machine, is a key piece of equipment that provides a dynamic oscillating culture environment in experiments such as microbial and cell culture. It mainly enhances oxygen transfer and dissolution in the culture medium through continuous oscillation, improves nutrient mixing, prevents cell sedimentation, and provides stable temperature conditions for microbial growth and metabolism. It is mainly divided into two types: reciprocating and rotary.

[0003] Current culture flasks can be effectively shaken using a shaker, but there is a problem in actual use. Specifically, the traditional shaking process of culture flasks requires a fastening mechanism to limit the movement. To improve the adaptability of the traditional limiting mechanism to the culture flask, it usually uses several adjustable limiting rods to hold the outside of the flask. If frequent operation is required, the limiting rods need to be adjusted at a high frequency, which reduces the convenience of limiting the flask. In view of this, the inventors urgently need to design a culture flask limiting component to improve the convenience of limiting the flask. Utility Model Content

[0004] Based on this, the purpose of this utility model is to provide a CAR-T cell expansion culture flask shaker to solve the technical problem that traditional shakers use multiple adjustable abutment rods to fix culture flasks of different sizes, which seriously affects efficiency when frequent culture flask replacement is required due to repeated adjustments.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a CAR-T cell expansion culture flask shaker, comprising a shaker body, a placement frame connected above the shaker body via a shaking mechanism, and a limiting mechanism above the placement frame to abut and limit several groups of culture flasks; The limiting mechanism includes four sets of insert sleeves, which are fixedly installed at the four upper corners of the placement frame. Insert rods are fitted into the inner side of each insert sleeve. Two sets of fixing plates are fixedly installed above each insert rod. Two sets of mounting rods are movably installed on each fixing plate. Several sets of limiting abutment rods are movably installed on each mounting rod. A gap is formed between adjacent limiting abutment rods for limiting and abutting the culture bottle.

[0006] By adopting the above technical solution, the culture flask is positioned, and the traditional integral fixed frame is decomposed into independently adjustable components. This allows operators to flexibly adjust the spatial position of each limiting abutment rod according to the actual size and shape of the culture flask, thereby forming a customized limiting space that precisely fits the flask body. This not only improves the adapter's wide compatibility with culture flasks of different specifications and manufacturers, but also effectively prevents the culture flask from tipping over or sliding during shaking, ensuring the stability and safety of the CAR-T cell expansion process.

[0007] Furthermore, a guide slope is formed on the outer periphery of the bottom end of the plug rod for guiding the plug rod into the inner side of the plug sleeve.

[0008] By adopting the above technical solution, the installation steps of the limiting mechanism are greatly simplified. Operators can easily complete the insertion without precise alignment, reducing the requirements for operator proficiency. Even in biosafety cabinets or clean benches with limited space and visibility, rapid, blind-operation-style precise positioning can be achieved, thereby significantly improving the convenience of the entire equipment assembly and the user experience.

[0009] Furthermore, the fixing plate has an installation groove, which is a straight groove structure. The mounting rod passes through the inner side of the installation groove and is assembled and connected to the fixing plate through a first fastening handwheel. The installation groove is a longitudinal position adjustment groove for the mounting rod.

[0010] By adopting the above technical solution, operators can flexibly adjust the height of the entire limiting and abutting rod system according to the height of the culture bottle. This is crucial for adapting to culture bottles with different capacities and length-to-diameter ratios, ensuring that the limiting force can be accurately applied to the most stable and suitable part of the bottle body, and avoiding problems such as insecure fixation or poor stability caused by the limiting point being too high or too low.

[0011] Furthermore, the limiting abutment rod has a through hole, and the mounting rod passes through the inside of the through hole. The limiting abutment rod is assembled and connected to the mounting rod through a second fastening handwheel. The limiting abutment rod can be adjusted along the mounting rod to adapt to the size of the culture bottle.

[0012] By adopting the above technical solution, each limiting abutment rod can slide independently on the mounting rod, thereby flexibly changing the width of the gap between adjacent limiting abutment rods, forming a tight enclosure structure for both the lower part of a small-diameter bottle and the middle part of a large-diameter bottle.

[0013] Furthermore, the culture flask is a conical culture flask, and the limiting abutment rod abuts against the conical surface of the culture flask.

[0014] By adopting the above technical solution, its use in the specific application scenario of CAR-T cell expansion is emphasized. Conical culture flasks, due to their large surface area / volume ratio, are conducive to gas exchange and are the preferred containers for large-scale expansion of suspended cells. This adapter limits the conical structure, and the force is applied directly to the high-strength conical surface area, avoiding stress concentration that may be caused to weak parts such as the bottom or neck of the flask.

[0015] Furthermore, the fixing plate and the mounting rod are made of stainless steel, and the limiting abutment rod is made of rubber.

[0016] By adopting the above technical solutions, stainless steel materials can effectively resist corrosion, rust and deformation under long-term, high-frequency shaking operation and possible disinfection and sterilization environments, ensuring the geometric accuracy and stability of the entire limiting structure, thereby maintaining long-term reliable limiting performance.

[0017] Furthermore, a control panel is provided on the front side of the shaking table body, the control panel including a digital display screen and control knobs.

[0018] By adopting the above technical solution, the digital display screen can display the current operating parameters of the shaker in real time and clearly, such as rotation speed and running time, so that the operator can intuitively monitor the amplification process. The control knob provides a precise and convenient way to set parameters, so as to quickly adjust to the preset optimal shaking conditions to meet the different shaking speed requirements that CAR-T cells may have at different amplification stages.

[0019] In summary, the present invention has the following main advantages: 1. This utility model uses a limiting mechanism. When it is necessary to change to the next batch of culture bottles and perform a shaking operation, it is only necessary to lift the limiting mechanism to separate the insertion rod from the insertion sleeve. Then, the culture bottle is placed on the placement frame, and then the insertion rod is re-inserted into the insertion sleeve to complete the abutment and limiting of the new culture bottle. This replaces the traditional operation of frequently adjusting the limiting abutment rod, improving the efficiency and convenience of changing to the next batch of culture bottles. 2. In this utility model, the functional adaptability and operational safety of the equipment are ensured through multiple adjustable structures. Structurally, the mounting rod can be adjusted in height along the vertical mounting groove, and the limiting abutment rod can be adjusted in radial position along the horizontal mounting rod. This two-dimensional adjustment capability enables it to accurately adapt to different specifications, especially conical culture bottles, and achieve stable customized limiting. Attached Figure Description

[0020] Figure 1 This is a three-dimensional structural diagram of the present invention; Figure 2 This is a schematic diagram of the exploded structure of this utility model; Figure 3This is a top view of the limiting mechanism of this utility model; Figure 4 This is a three-dimensional structural diagram of the limiting mechanism of this utility model.

[0021] In the diagram: 1. Shaker body; 2. Placement frame; 3. Control panel; 4. Culture flask; 5. Limiting mechanism; 501. Insertion sleeve; 502. Insertion rod; 503. Fixing plate; 504. Mounting groove; 505. Mounting rod; 506. First fastening handwheel; 507. Limiting abutment rod; 508. Second fastening handwheel; 509. Gap. Detailed Implementation

[0022] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0023] In this embodiment:

[0024] A CAR-T cell expansion culture flask shaker, such as Figure 1-4 As shown, it includes a shaker body 1, and a placement frame 2 is connected to the top of the shaker body 1 via a shaking mechanism. The placement frame 2 is positioned above a number of culture bottles 4 by a limiting mechanism 5. The limiting mechanism 5 includes four sets of insert sleeves 501, which are fixedly installed at the four upper corners of the placement frame 2. Insertion rods 502 are adapted to be inserted into the inner side of the insert sleeves 501. Two sets of fixing plates 503 are fixedly installed above the insertion rods 502. Two sets of mounting rods 505 are movably installed on the fixing plates 503. Several sets of limiting abutment rods 507 are movably installed on the mounting rods 505. A gap 509 is formed between adjacent limiting abutment rods 507 for limiting and abutting the culture bottle 4.

[0025] This design achieves precise positioning of the culture flask 4, breaking down the traditional monolithic fixing frame into independently adjustable components. This allows operators to flexibly adjust the spatial position of each limiting abutment rod 507 according to the actual size and shape of the culture flask 4, thus forming a customized limiting space that precisely fits the flask body. This not only improves the adapter's wide compatibility with culture flasks 4 of different specifications and manufacturers, but also effectively prevents the culture flask 4 from tipping over or sliding during shaking, ensuring the stability and safety of the CAR-T cell expansion process. At the same time, the limiting mechanism 5 uses plug-in connection as the main connection method with the placement frame 2, bringing a revolutionary improvement in operational efficiency. When changing batches, there is no need to perform tedious repeated fine-tuning of each limiting abutment rod 507; simply lift the entire limiting mechanism 5 as a whole to quickly pick up and place the culture flask 4, and then re-plug and fix it.

[0026] See Figure 1 , Figure 2 , Figure 4 The bottom outer periphery of the insertion rod 502 is formed with a guide slope, which is used to guide the insertion rod 502 into the inner side of the insertion sleeve 501. This greatly simplifies the installation steps of the limiting mechanism 5. Operators can easily complete the insertion without precise alignment, reducing the requirements for operator proficiency. Even in biosafety cabinets or clean benches with limited space and visibility, it can achieve rapid, blind operation and precise positioning, thereby significantly improving the convenience of the entire equipment assembly and user experience. At the same time, the guide slope also brings the benefits of long-term stability and reliability, ensuring that each insertion is smooth and in place, avoiding mechanical damage to the insertion sleeve 501 or insertion rod 502 that may be caused by forced insertion, and extending the service life of key components.

[0027] See Figure 1 , Figure 2 , Figure 4 The fixing plate 503 has an installation groove 504, which is a straight groove structure. The installation rod 505 passes through the inner side of the installation groove 504 and is assembled and connected to the fixing plate 503 through the first fastening handwheel 506. The installation groove 504 is a longitudinal position adjustment groove for the installation rod 505, which allows the operator to flexibly adjust the height position of the entire limiting abutment rod 507 system according to the height of the culture bottle 4. This is crucial for adapting to culture bottles 4 with different capacities and length-to-diameter ratios, ensuring that the limiting force can be accurately applied to the most stable and suitable part of the bottle body, avoiding problems such as insecure fixation or poor stability caused by the limiting point being too high or too low. At the same time, this adjustment method combines flexibility and stability. The straight groove structure of the installation groove 504 ensures the linearity and smoothness of the adjustment process, while the first fastening handwheel 506 provides an intuitive operation and a strong locking force. After adjusting to the appropriate height, tightening the handwheel ensures that the installation rod 505 will not move during shaking, ensuring the absolute reliability of the limiting.

[0028] See Figure 1 , Figure 2 , Figure 3 , Figure 4The limiting abutment rod 507 has a through hole, and the mounting rod 505 passes through the inside of the through hole. The limiting abutment rod 507 is assembled and connected to the mounting rod 505 through the second fastening handwheel 508. The limiting abutment rod 507 can be adjusted along the mounting rod 505 to adapt to the size of the culture bottle 4. Each limiting abutment rod 507 can slide independently on the mounting rod 505, thereby flexibly changing the width of the gap 509 between adjacent limiting abutment rods 507. Whether for the lower part of a small-diameter bottle or the middle part of a large-diameter bottle, a tight enclosure structure can be formed. At the same time, the independent horizontal adjustment capability combined with the vertical adjustment capability of the mounting rod 505 constitutes a three-dimensional adjustable flexible limiting space. This limiting mechanism 5 can adapt to the change in diameter, making the fixation of the culture bottle 4 more stable.

[0029] See Figure 1 , Figure 2 Culture flask 4 is a conical culture flask, with the limiting abutment rod 507 abutting against the conical surface of culture flask 4, emphasizing its use in the specific application scenario of CAR-T cell expansion. Conical culture flasks, due to their large surface area / volume ratio, are conducive to gas exchange and are the preferred containers for large-scale expansion of suspended cells. This adapter limits the conical structure, and the force is applied directly to the high-strength conical surface area, avoiding stress concentration that may occur to weak parts such as the bottom or neck of the flask. At the same time, the abutment against the conical surface minimizes interference with the shaking pattern of the culture medium inside the flask, ensuring that the shaking of culture flask 4 is synchronized with the drive of the shaker body 1, providing a stable and uniform supply of nutrients and oxygen to the CAR-T cells inside, which is crucial for obtaining highly active and high-yield cell products.

[0030] See Figure 1 , Figure 2 , Figure 3 , Figure 4 The fixing plate 503 and the mounting rod 505 are made of stainless steel, while the limiting abutment rod 507 is made of rubber. Under long-term, high-frequency shaking and possible sterilization environments, the stainless steel material can effectively resist corrosion, rust, and deformation, ensuring the geometric accuracy and stability of the entire limiting structure, thereby maintaining long-term reliable limiting performance. At the same time, the limiting abutment rod 507 is made of rubber. The softness of rubber can prevent hard metal parts from directly contacting and scratching the surface of the culture bottle 4, protecting expensive consumables. The high coefficient of friction of rubber provides excellent anti-slip effect, ensuring that even under high-speed shaking, the culture bottle 4 will not slide relative to the limiting rod. The rubber material has a certain buffering and shock absorption effect, which can absorb some of the instantaneous impact force generated by shaking, creating a gentler mechanical environment for fragile cells.

[0031] See Figure 1 , Figure 2The front side of the shaker body 1 is equipped with a control panel 3, which includes a digital display screen and control knobs. The digital display screen can clearly display the current operating parameters of the shaker in real time, such as rotation speed and running time, so that the operator can intuitively monitor the amplification process. The control knobs provide a precise and convenient way to set parameters, so as to quickly adjust to the preset optimal shaking conditions to meet the different shaking speed requirements that CAR-T cells may have at different amplification stages. At the same time, the integrated control panel 3 is an important manifestation of the intelligence and standardization of modern laboratory equipment, avoiding the mess of cables and inconvenience of operation caused by external controllers. Precise speed control is crucial for the growth of CAR-T cells. Too low a rotation speed may lead to uneven mixing, while too high a rotation speed may generate shear force that damages the cells.

[0032] The implementation principle of this embodiment is as follows: The operating parameters of the shaker body 1 are set via the control panel 3. Then, the culture flasks 4 to be amplified are placed on the placement frame 2 driven by the shaking mechanism. The fixing process relies on the limiting mechanism 5. The main frame is established by the four corner-fixed insertion sleeves 501 and insertion rods 502. By loosening the first fastening handwheel 506, the longitudinal height of the mounting rod 505 in the mounting groove 504 of the fixing plate 503 can be adjusted. By loosening the second fastening handwheel 508, the height of each rod can be slidably adjusted. The horizontal position of the limiting abutment rod 507 on the mounting rod 505 is adjusted in both longitudinal and transverse directions to ensure that the gap 509 between the multiple limiting abutment rods 507 precisely matches the shape of the culture bottle 4. The rubber limiting abutment rods 507 gently and firmly hold the bottle body. Finally, tightening all handwheels completes the fixation. After starting, the shaker body 1 drives the placement frame 2 and the culture bottle 4, which is safely limited, to gently shake together through the shaking mechanism, providing an ideal expansion environment for CAR-T cells.

[0033] Although embodiments of the present invention have been shown and described, these specific embodiments are merely explanations of the present invention and are not intended to limit the invention. The specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. After reading this specification, those skilled in the art may make modifications, substitutions, and variations to the embodiments as needed without departing from the principles and spirit of the present invention, provided that such modifications, substitutions, and variations are within the scope of the claims of the present invention and are protected by patent law.

Claims

1. A CAR-T cell expansion culture flask shaker, characterized in that: Includes a shaker body (1), and a placement frame (2) is connected above the shaker body (1) via a shaking mechanism. A limiting mechanism (5) is used above the placement frame (2) to abut and limit several groups of culture bottles (4). The limiting mechanism (5) includes four sets of insert sleeves (501). The four sets of insert sleeves (501) are fixedly installed at the four upper corners of the placement frame (2). Insert rods (502) are adapted to be inserted into the inner side of the insert sleeves (501). Two sets of fixing plates (503) are fixedly installed above the insert rods (502). Two sets of mounting rods (505) are movably installed on the fixing plates (503). Several sets of limiting abutment rods (507) are movably installed on the mounting rods (505). A gap (509) is formed between adjacent limiting abutment rods (507) for limiting and abutting the culture bottle (4).

2. The CAR-T cell expansion culture flask shaker according to claim 1, characterized in that: The bottom outer periphery of the plug rod (502) is formed with a guide slope for guiding the plug rod (502) into the inner side of the plug sleeve (501).

3. The CAR-T cell expansion culture flask shaker according to claim 1, characterized in that: The fixing plate (503) has an installation groove (504) with a straight groove structure. The mounting rod (505) passes through the inner side of the mounting groove (504) and is assembled and connected to the fixing plate (503) through the first fastening handwheel (506). The mounting groove (504) is a longitudinal position adjustment groove for the mounting rod (505).

4. The CAR-T cell expansion culture flask shaker according to claim 1, characterized in that: The limiting abutment rod (507) has a through hole, and the mounting rod (505) passes through the inside of the through hole. The limiting abutment rod (507) is assembled and connected to the mounting rod (505) through the second fastening handwheel (508). The limiting abutment rod (507) can be adjusted along the mounting rod (505) to adapt to the size of the culture bottle (4).

5. The CAR-T cell expansion culture flask shaker according to claim 1, characterized in that: The culture bottle (4) is a conical culture bottle, and the limiting abutment rod (507) abuts against the conical surface of the culture bottle (4).

6. The CAR-T cell expansion culture flask shaker according to claim 1, characterized in that: The fixing plate (503) and the mounting rod (505) are made of stainless steel, and the limiting abutment rod (507) is made of rubber.

7. The CAR-T cell expansion culture flask shaker according to claim 1, characterized in that: The front side of the shaking table body (1) is provided with a control panel (3), which includes a digital display screen and control knobs.

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