A polyamine derivatization device for visualizing ph

Abstract

The utility model discloses a kind of polyamine derivatization devices of visual pH value, it is related to polyamine detection technical field;To solve the problem that sample consumption is large in the existing detection process, pH value detection is influenced by air oxidation, leading to inaccurate value and unable to monitor the dynamic change of pH in the process of polyamine derivatization in real time, the following technical scheme is proposed: including pH detection component and constant temperature maintaining body;pH detection component includes tube body, tube cover and micro pH meter, the upper portion open end of tube body is provided with outer thread, tube body is connected with tube cover by thread, a through hole is opened in the center of tube cover: micro pH meter includes a detection shell and sample introduction channel extending downward from the detection shell.The utility model solves the problem that sample consumption is large in the existing detection process, pH value detection is influenced by air oxidation, leading to inaccurate value and unable to monitor the dynamic change of pH in the process of polyamine derivatization in real time.

Description

Technical Field

[0001] This invention relates to the field of polyamine detection technology, specifically to a polyamine derivatization device for visualizing pH values. Background Technology

[0002] Accurate pH measurement is crucial in the detection of polyamine content. Currently, pH measurement typically requires manual adjustment and is obtained by dropping liquid onto test strips.

[0003] This traditional method has significant drawbacks: First, existing detection processes often require large sample volumes; second, during operation, liquid samples are exposed to air and are easily affected by oxidation, leading to inaccurate pH values, which severely impacts the final accuracy of polyamine content detection results; furthermore, methods such as test strip titration cannot provide real-time dynamic monitoring of pH changes during polyamine derivatization. Therefore, existing technologies urgently need to address the problems of high sample consumption, inaccurate pH readings due to air oxidation, and the inability to monitor dynamic pH changes in real time. Utility Model Content

[0004] The purpose of this invention is to provide a polyamine derivatization device that visualizes pH values, in order to solve the problems of large sample consumption, inaccurate pH values ​​due to air oxidation, and inability to monitor dynamic pH changes in real time during polyamine derivatization in existing detection processes.

[0005] The technical solution of this utility model to solve the above-mentioned technical problems is as follows: A polyamine derivatization device for visualizing pH values ​​includes a pH detection component and a temperature-maintaining body; The pH detection assembly includes a tube body, a cap 5, and a micro pH meter. The upper open end of the tube body 9 is provided with an external thread 8, and the tube body 9 is connected to the cap 5 by the thread. A through hole is opened in the center of the cap 5. The micro pH meter includes a detection housing and a sample inlet channel 6 extending downward from the detection housing; the detection housing is detachably mounted on top of the tube cap 5 by a snap-fit ​​structure, and the sample inlet channel 6 passes through the through hole of the tube cap 5; The constant temperature maintainer includes a temperature control seat 12 and a base connected to the temperature control seat 12. The temperature control seat 12 is provided with a groove 11 for accommodating the tube 9. The temperature control seat 12 is also provided with a semiconductor heat preservation device located on the outer wall of the groove 11. A storage battery 13 is provided inside the base.

[0006] Furthermore, a negative pressure suction pump 1 and a transparent detection chamber 3 are sequentially arranged inside the detection housing; the negative pressure suction pump 1 is located above the transparent detection chamber 3, and the negative pressure suction pump 1 and the transparent detection chamber 3 are connected.

[0007] Furthermore, the transparent testing chamber 3 is equipped with a colorimetric card 2.

[0008] Furthermore, the transparent detection chamber 3 is a hollow cavity, and its inner wall is loaded with a polyamine composite membrane 4.

[0009] Furthermore, the polyamine composite membrane 4 is composed of a base layer, a crosslinking layer, a polyamine immobilization layer, and a protective layer stacked sequentially.

[0010] Furthermore, the sample injection channel 6 is equipped with a hydrogenated nitrile rubber pad 7.

[0011] Furthermore, the space between the grooves 11 in the temperature control seat 12 is filled with hydrogel particles 10, which serve as thermal insulation material.

[0012] Furthermore, a charging port 14 is provided on the base of the constant temperature maintainer, and the charging port 14 is electrically connected to the storage battery 13.

[0013] This invention has the following beneficial effects: By setting up a micro pH meter for micro-absorption, this invention greatly reduces the amount of sample consumed. By utilizing a sealed detection environment, it solves the problem that samples exposed to air are easily affected by oxidation when obtaining pH values ​​by dripping liquid onto test paper in the traditional method. This achieves accurate pH value detection, improves the accuracy and efficiency of polyamine content detection, and solves the problem of non-visualization of pH value in the current polyamine derivatization process through the visualization structure of the micro pH meter and colorimetric card. It can dynamically monitor the changes in pH value during the polyamine derivatization process. Attached Figure Description

[0014] Figure 1 A schematic diagram of a micro pH meter, an embodiment of a polyamine derivatization device for visualizing pH values; Figure 2 A schematic diagram of the tube structure of an embodiment of a polyamine derivatization device for visualizing pH values; Figure 3 A cross-sectional view of the transparent detection chamber structure of an embodiment of a polyamine derivatization device for visualizing pH values; Figure 4 This is a schematic diagram of the isothermal maintenance structure of an embodiment of a polyamine derivatization device for visualizing pH values.

[0015] Figures 1 to 4 The reference numerals in the attached figures represent: 1-negative pressure suction pump, 2-colorimetric card, 3-transparent detection chamber, 4-polyamine composite membrane, 5-tube cap, 6-sample inlet channel, 7-hydrogenated nitrile rubber pad, 8-external thread, 9-tube body, 10-hydrogel particles, 11-groove, 12-temperature control base, 13-storage battery, 14-charging port. Detailed Implementation

[0016] The technical solution of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0017] like Figure 1-3 As shown, this embodiment of the present invention provides a polyamine derivatization device for visualizing pH values, including a pH detection component and a constant temperature maintenance body.

[0018] The pH detection assembly includes a tube body 9, a cap 5, and a micro pH meter. In this embodiment, the tube body 9 is preferably a 15ml centrifuge tube, with a length of 12cm, an outer diameter of 1.7cm, an inner diameter of 1.5cm, and a thickness of 0.1cm. The upper open end of the tube body 9 has an external thread 8. The cap 5 is used to seal with the tube body 9; its height is 1.02cm, its inner diameter is 1.7cm, its outer diameter is 2.1cm, and its thickness is 0.2cm. The cap 5 is threadedly connected to the external thread 8 of the tube body 9 via its internal thread, achieving a sealing effect. The total length of the cap 5 and the tube body 9 is 12.1cm. A through hole is formed in the center of the cap 5.

[0019] The micro pH meter includes a detection housing and a sample inlet channel 6 extending downward from the detection housing. The detection housing is detachably mounted on top of the tube cap 5 via a snap-fit ​​structure. Inside the detection housing, from top to bottom, are arranged a negative pressure suction pump 1 and a transparent detection chamber 3. The negative pressure suction pump 1 is located above the transparent detection chamber 3, and the two are connected. The sample inlet channel 6 passes through the through-hole of the tube cap 5 and extends into the interior of the tube body 9. The sample inlet channel 6 is made of acid and alkali resistant polytetrafluoroethylene. Furthermore, the interior of the sample inlet channel 6 is equipped with a backflow-preventing and acid and alkali resistant hydrogenated nitrile rubber gasket 7.

[0020] The transparent detection chamber 3 is a cylindrical cavity. A colorimetric card 2 is installed inside the transparent detection chamber 3. The color patches on the colorimetric card 2 correspond linearly to the pH value, with a color difference range of ΔE = 5.2-8.7. A polyamine composite membrane 4 (i.e., polyamine molecules covalently fixed to a porous silica matrix) is loaded onto the inner wall of the transparent detection chamber 3. This polyamine composite membrane 4 consists of four layers stacked sequentially: a base layer (silica), a crosslinking layer (3-aminopropyltriethoxysilane), a polyamine immobilization layer (spermine-glutaraldehyde polymer), and a protective layer (polyethersulfone microporous membrane).

[0021] As attached Figure 4As shown, the temperature-maintaining body is rectangular, including a temperature control base 12 and a base connected to the temperature control base 12. The temperature control base 12 has 12 grooves 11 for accommodating the tube 9. The spacing between these grooves 11 should be greater than 2.5 cm, and the groove depth is 10 cm. Hydrogel particles 10, serving as insulation material, are filled in the gaps between the grooves 11 within the temperature control base 12. A semiconductor insulation device, serving as a temperature control mechanism, is also provided on the temperature control base 12, located on the outer wall of the grooves 11. The base is located below the temperature control base 12, and a storage battery 13 is disposed within the base. Furthermore, a charging port 14 is provided on the base, which is electrically connected to the storage battery 13.

[0022] The working process and usage method of this utility model are as follows: The tube body 9 containing the sample is screwed tightly to the tube cap 5, which is equipped with a micro pH meter, to form a sealed pH detection assembly. This pH detection assembly is placed in the groove 11 of the constant temperature maintenance body. The semiconductor heat preservation device (i.e., temperature control device) located on the outer wall of the groove 11 is activated to maintain the constant temperature of the tube body 9. During the derivatization process, the negative pressure suction pump 1 of the micro pH meter is activated, and the sample in the tube body 9 is aspirated, passes through the anti-backflow hydrogenated nitrile rubber pad 7, and enters the transparent detection chamber 3 through the sample inlet channel 6. After the sample is injected, it is allowed to stand for 30 seconds to allow the sample to fully contact and react with the polyamine composite membrane 4 on the inner wall of the transparent detection chamber 3. The polyamine composite membrane 4 changes color according to the pH value of the sample. Finally, the colorimetric card 2 located inside the transparent detection chamber 3 changes to the corresponding color, thus achieving accurate and visual dynamic monitoring of the pH value during the sealed and constant temperature derivatization process.

[0023] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A polyamine derivatization device for visualizing pH values, characterized in that, Includes pH detection components and a temperature-maintaining body; The pH detection assembly includes a tube body (9), a tube cap (5), and a micro pH meter. The upper open end of the tube body (9) is provided with an external thread (8). The tube body (9) is connected to the tube cap (5) by the thread. A through hole is opened in the center of the tube cap (5). The micro pH meter includes a detection housing and a sample inlet channel (6) extending downward from the detection housing; the detection housing is detachably mounted on the top of the tube cap (5) by a snap-fit ​​structure, and the sample inlet channel (6) passes through the through hole of the tube cap (5); The constant temperature maintainer includes a temperature control seat (12) and a base connected to the temperature control seat (12). The temperature control seat (12) is provided with a groove (11) for accommodating the tube (9). The temperature control seat (12) is also provided with a semiconductor heat preservation device located on the outer wall of the groove (11). A storage battery (13) is provided inside the base.

2. The polyamine derivatization device for visualizing pH values ​​according to claim 1, characterized in that, The inside of the detection housing is arranged in sequence a negative pressure suction pump (1) and a transparent detection chamber (3); the negative pressure suction pump (1) is located above the transparent detection chamber (3), and the negative pressure suction pump (1) and the transparent detection chamber (3) are connected.

3. The polyamine derivatization device for visualizing pH values ​​according to claim 2, characterized in that, The transparent testing chamber (3) is equipped with a colorimetric card (2).

4. The polyamine derivatization device for visualizing pH values ​​according to claim 2, characterized in that, The transparent detection chamber (3) is a hollow cavity with a polyamine composite membrane (4) loaded on its inner wall.

5. The polyamine derivatization device for visualizing pH values ​​according to claim 4, characterized in that, The polyamine composite membrane (4) is composed of a base layer, a crosslinking layer, a polyamine fixing layer and a protective layer stacked in sequence.

6. The polyamine derivatization device for visualizing pH values ​​according to claim 1, characterized in that, The sample inlet channel (6) is provided with a hydrogenated nitrile rubber pad (7).

7. The polyamine derivatization device for visualizing pH values ​​according to claim 1, characterized in that, The temperature control seat (12) is filled with hydrogel particles (10) as thermal insulation material in the gaps between the grooves (11).

8. The polyamine derivatization device for visualizing pH values ​​according to claim 1, characterized in that, The base of the constant temperature maintainer is also provided with a charging port (14), which is electrically connected to the storage battery (13).

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