Sample homogenizing device for visual detection of dragon fruit virus

Abstract

The utility model relates to pitaya sample homogenate technical field discloses a kind of sample homogenate devices for pitaya virus visualization detection, including homogenate box, cover plate, bag clamping mechanism and homogenate mechanism, bag clamping mechanism is used to seal and fix disposable homogenization bag between two cover plates, homogenate mechanism includes extrusion frame and extrusion roller driven by electric telescopic handle, disposable homogenization bag is reciprocatingly beaten outside in homogenate box, realizes contactless homogenate.The utility model avoids the cross contamination between sample from structure and exempts from equipment cleaning procedure by the contactless homogenate mode of full closure;Meanwhile, observation window and adjustable operation platform are set, the visualization and accurate control of homogenate process are realized, and the sample processing quality is guaranteed.

Description

Technical Field

[0001] This utility model relates to the field of dragon fruit sample homogenization technology, and in particular to a sample homogenization device for visual detection of dragon fruit viruses. Background Technology

[0002] In biotechnology, especially in the field of virus detection, sample pretreatment is a crucial step. Among these steps, homogenization is a key process for obtaining the analyte. Commonly used homogenization equipment employs a high-speed rotating blade to directly homogenize the sample in a homogenizing cup. This method requires the blade and homogenizing cup to undergo rigorous cleaning and sterilization procedures after each use. Otherwise, residual nucleic acid or protein substances from the previous sample may remain, causing cross-contamination when processing the next sample. For applications like virus detection that require high sensitivity and accuracy, even trace amounts of cross-contamination can lead to false positive results, severely impacting the reliability of the detection. The high-speed rotating blade generates localized high temperatures and enormous mechanical shear forces during sample homogenization. This can damage the activity of structurally unstable viral nucleic acids (such as RNA) or target proteins, thereby reducing the sensitivity and success rate of subsequent detection.

[0003] Therefore, this invention proposes a sample homogenization device for visual detection of dragon fruit virus to address the shortcomings of existing technologies. Utility Model Content

[0004] In view of the problems in the existing sample homogenizing device, such as the homogenizing component being in direct contact with the sample, leading to cross-contamination between samples, and the cumbersome and complicated equipment cleaning and sterilization process, this utility model aims to provide a sample homogenizing device for visual detection of dragon fruit virus with an improved structure that can effectively solve the above problems.

[0005] This utility model provides a sample homogenization device for visual detection of dragon fruit virus, including: a homogenization box, two cover plates, a homogenization mechanism, and a bag clamping mechanism.

[0006] The homogenizing mechanism includes an extrusion frame, an electric telescopic rod, and an extrusion roller mounted on the extrusion frame. The extrusion frame is provided with a slider that slides in cooperation with the groove of the homogenizing box.

[0007] Furthermore, the inner wall of the homogenizing tank is provided with a sliding groove, and the extrusion frame is slidably disposed in the sliding groove via the slider. The electric telescopic rod is connected to the extrusion frame to drive the extrusion frame to reciprocate. The bag clamping mechanism is used to clamp and seal the disposable homogenizing bag between two openable cover plates disposed on the top of the homogenizing tank, so that the clamped disposable homogenizing bag can be patted and homogenized by the extrusion roller.

[0008] Preferably, the slider is further provided with a locking block, which is used to prevent the slider from falling out of the groove.

[0009] Preferably, there are two extrusion frames arranged opposite each other, and the number and distribution of the extrusion rollers on the two extrusion frames are different.

[0010] Preferably, the homogenizing tank is provided with a groove, and the bottom of the cover plate is provided with a sliding block that slides in conjunction with the groove, for adjusting the distance between the two cover plates.

[0011] Preferably, the bag clamping mechanism includes a compression plate disposed on one of the cover plates, and a slot disposed on another cover plate for engaging with the compression plate to clamp the disposable homogenizing bag.

[0012] Preferably, the bag clamping mechanism further includes a buckle retainer and a pipe clamp respectively disposed on the two cover plates, wherein the buckle retainer and the pipe clamp engage with each other to lock the two cover plates.

[0013] Preferably, the homogenizer is provided with a transparent observation window.

[0014] Preferably, the device further includes an operating console electrically connected to the electric telescopic rod, used to control the start, stop, extension frequency, or stroke of the electric telescopic rod.

[0015] This utility model has the following beneficial effects: 1. This utility model, by setting up a bag clamping mechanism and a homogenizing mechanism, seals and fixes the disposable homogenizing bag containing the sample, and the homogenizing mechanism beats and squeezes the sample from the outside of the homogenizing bag. This solves the problem that the homogenizing parts of the existing homogenizing device are in direct contact with the sample, which will lead to cross-contamination between samples and make the equipment difficult to clean thoroughly. It achieves the technical effect of avoiding cross-contamination from the structure, eliminating the need for equipment cleaning, and ensuring the biological activity of the sample, which significantly improves the safety and efficiency of sample pretreatment.

[0016] 2. This utility model solves the problems of insufficient or excessive homogenization caused by setting an observation window on the homogenizing box, cooperating with an operating table with adjustable electric telescopic rod extension frequency and stroke, and designing the extrusion rollers to be asymmetrically distributed. This solves the problems of the homogenization process being invisible, parameters being fixed, and difficulty in real-time adjustment according to the sample state in the prior art. It achieves the technical effect of controllable homogenization process, uniform homogenization effect, and flexible adjustment according to actual needs, thus ensuring the quality of sample processing. Attached Figure Description

[0017] Figure 1 This is a perspective view of a sample homogenizer for visual detection of dragon fruit virus proposed in this utility model; Figure 2 This is a cross-sectional view of the homogenizing tank in a sample homogenizing device for visual detection of dragon fruit virus proposed in this utility model. Figure 3 This is an exploded view of the homogenization mechanism of a sample homogenization device for visual detection of dragon fruit virus proposed in this utility model. Figure 4 This is an exploded view of the bag clamping mechanism in a sample homogenizer for visual detection of dragon fruit virus proposed in this utility model. Figure 5 This is a split view of the extrusion plate in a sample homogenizer for visual detection of dragon fruit virus proposed in this utility model.

[0018] Legend: 1. Homogenizing tank; 2. Cover plate; 3. Homogenizing mechanism; 31. Electric telescopic rod; 32. Extrusion frame; 33. Slider; 34. Slide groove; 35. Clamping block; 36. Extrusion roller; 37. Observation window; 38. Operating table; 4. Bag clamping mechanism; 41. Sliding block; 42. Groove; 43. Extrusion plate; 44. Clamping slot; 45. Buckle fastener; 46. Pipe clamp. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of this utility model clearer, the technical solutions in the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model. Example

[0020] Please refer to Figure 1 and Figure 2 A sample homogenization device for visual detection of dragon fruit virus includes a homogenization box 1 as an integral mounting frame, a homogenization mechanism 3 disposed inside the homogenization box 1, two closable cover plates 2 disposed on the top of the homogenization box 1, and a bag clamping mechanism 4 integrated on the cover plates 2. A transparent observation window 37 is provided on the body of the homogenization box 1. The device also includes an operating table 38 electrically connected to the homogenization mechanism 3. An integrally formed groove 34 for sliding the homogenization mechanism 3 and a recess 42 for sliding the cover plates 2 are formed on the inner wall of the homogenization box 1. Figure 3As shown, the homogenizing mechanism 3 includes two opposing extrusion frames 32. Multiple extrusion rollers 36 are fixedly connected to the extrusion frames 32. A slider 33, which slides in a groove 34, is fixedly connected to the outer wall of the extrusion frame 32. The slider 33 is also equipped with a locking block 35 to prevent it from dislodging from the groove 34. The homogenizing mechanism 3 also includes an electric telescopic rod 31 for driving the two extrusion frames 32 to move towards or away from each other. Figure 4 and Figure 5 As shown, the bottom of the cover plate 2 is fixedly connected to a sliding block 41 that slides in cooperation with the groove 42 of the homogenizing box 1. The bag clamping mechanism 4 includes a squeezing plate 43 set on one cover plate 2, a slot 44 set on another cover plate 2, and a buckle fastener 45 and a pipe clamp 46 respectively set on the two cover plates 2 for mutual locking.

[0021] Please refer to Figure 2 and Figure 3 The homogenizing mechanism 3 includes two extrusion frames 32 arranged opposite each other along the width direction of the homogenizing tank 1. Each extrusion frame 32 is integrally formed as a frame structure. On the inner side wall of the extrusion frame 32 facing the center of the homogenizing tank 1, multiple freely rotatable extrusion rollers 36 are installed by means of fixed shafts. The number and arrangement of the extrusion rollers 36 on the two extrusion frames 32 are different. This asymmetrical arrangement allows for multi-angle and non-repetitive beating and extrusion of the disposable homogenizing bag during reciprocating motion. On the outer side wall of the extrusion frame 32 away from the center of the homogenizing tank 1, at least two sliders 33 distributed along the height direction of the extrusion frame 32 are fixedly connected. Corresponding openings are made on the inner wall of the homogenizing tank 1. There are two parallel slide grooves 34. In the assembled state, the slider 33 of the extrusion frame 32 is slidably locked in the slide groove 34 of the homogenizing box 1. The slider 33 is also provided with a locking block 35. The outer contour of the locking block 35 is adapted to the opening contour of the slide groove 34 to prevent the slider 33 from coming out of the slide groove 34. This multi-point, linear sliding fit provides a stable guide for the reciprocating motion of the extrusion frame 32. The electric telescopic rod 31 serves as a power component. One end of it is fixedly connected to one extrusion frame 32, and the other end is fixedly connected to another extrusion frame 32. The operating table 38 is electrically connected to the electric telescopic rod 31 through a wire to precisely control the extension stroke and frequency of the electric telescopic rod 31.

[0022] Please refer to Figure 4 and Figure 5To achieve adaptable clamping of disposable homogenizing bags of different sizes, a groove 42 is provided along the length of the top of the homogenizing tank 1. Sliding blocks 41, which can slide along the groove 42, are fixedly connected to the bottom of both cover plates 2. The distance between the two cover plates 2 is adjusted by moving the cover plates 2. The specific structure of the bag clamping mechanism 4 is as follows: a pressing plate 43 is fixedly connected to the side wall of one cover plate 2 facing the other cover plate 2, and a slot 44 for accommodating and clamping the opening of the disposable homogenizing bag is provided on the corresponding side wall of the other cover plate 2. To further securely lock the two cover plates 2, the bag clamping mechanism 4 also... The device includes a snap fastener 45 and a pipe clamp 46, which are respectively fixedly connected to the top of the two cover plates 2. The structures of the snap fastener 45 and the pipe clamp 46 are compatible with each other and can lock each other in place, thereby applying sufficient clamping force to the two cover plates 2. In order to facilitate real-time monitoring of the homogenization process, a transparent observation window 37 is also opened and fixedly installed on the front wall of the homogenization tank 1. The device also includes an operating table 38, which integrates a control circuit and is electrically connected to the electric telescopic rod 31 through a wire. It is used to set and control the start, stop, extension frequency or stroke of the electric telescopic rod 31.

[0023] Working principle: Open the two cover plates 2, use the bag clamping mechanism 4, and use the two sliding blocks 41 at the bottom of the cover plate 2 to slide left and right inside the two grooves 42 of the homogenizing box 1 to adjust the position of the cover plate 2. Place the disposable homogenizing bag containing the dragon fruit pieces between the two cover plates 2, and then make the extrusion plate 43 at the bottom right side of the left cover plate 2 squeeze the disposable homogenizing bag so that it enters the slot 44 of the right cover plate 2. The top of the right cover plate 2 squeezes the disposable homogenizing bag so that it enters the slot 44 of the left cover plate 2, thereby initially stabilizing the disposable homogenizing bag. Use the two buckle fixing devices 45 and the two pipe clamps 46 to lock together so that the two cover plates 2 tightly and stably clamp the disposable homogenizing bag, ensuring that the disposable homogenizing bag will not loosen during the subsequent homogenization process, and providing stable bag fixation for the homogenization operation. The operating table 38 controls the start of the electric telescopic rod 31 in the homogenization mechanism 3. The electric telescopic rod 31 extends and retracts, driving the extrusion frame 32 to move left and right. During the movement of the extrusion frame 32, the slider 33 on the extrusion frame 32 slides left and right inside the groove 34 of the homogenization box 1, providing guidance for the movement of the extrusion frame 32. At the same time, the locking block 35 on the slider 33 prevents multiple sliders 33 from becoming loose, ensuring the stability of the movement of the extrusion frame 32. When the two extrusion frames 32 move inward, the multiple extrusion rollers 36 fixedly connected inside the extrusion frame 32 will beat the blocky dragon fruit inside the disposable homogenization bag. Since the number and distribution of the left and right extrusion rollers 36 are different, they can act evenly on the dragon fruit inside the bag, ensuring that the dragon fruit inside the bag is broken evenly, achieving the purpose of homogenization. During the homogenization process, the homogenization status of the dragon fruit inside the bag can be observed through the observation window 37 on the homogenization box 1, so that the extension frequency or stroke of the electric telescopic rod 31 can be adjusted through the operating table 38 according to the actual situation to ensure the homogenization effect. After homogenization is completed, the electric telescopic rod 31 is controlled to extend and retract in the opposite direction by the operating table 38, which drives the extrusion frame 32 to move outward, so that the extrusion roller 36 is separated from the disposable homogenization bag. Then, the buckle fastener 45 is released from the pipe clamp 46, the cover plate 2 is moved, and the homogenized dragon fruit disposable homogenization bag is taken out.

Claims

1. A sample homogenizer for visual detection of dragon fruit virus, comprising: A homogenizing tank (1) is provided with a groove (34) on its inner wall. Two openable and closable cover plates (2) are provided on the top of the homogenizing tank (1); Homogenizing mechanism (3); And the bag clamping mechanism (4); Its features are, The homogenizing mechanism (3) includes an extrusion frame (32), an electric telescopic rod (31), and an extrusion roller (36) disposed on the extrusion frame (32). The extrusion frame (32) is provided with a slider (33) that slides in cooperation with the slide groove (34). The electric telescopic rod (31) is connected to the extrusion frame (32) to drive the extrusion frame (32) to reciprocate along the slide groove (34). The bag clamping mechanism (4) is used to clamp and seal the disposable homogenizing bag between the two cover plates (2), so that the clamped disposable homogenizing bag can be patted and homogenized by the extrusion roller (36).

2. The sample homogenizer for visual detection of dragon fruit virus according to claim 1, characterized in that, The slider (33) is also provided with a locking block (35), which is used to prevent the slider (33) from coming out of the groove (34).

3. The sample homogenizer for visual detection of dragon fruit virus according to claim 1, characterized in that, There are two extrusion frames (32) arranged opposite each other, and the number and distribution of the extrusion rollers (36) on the two extrusion frames (32) are different.

4. The sample homogenizer for visual detection of dragon fruit virus according to claim 1, characterized in that, The homogenizing tank (1) is provided with a groove (42), and the bottom of the cover plate (2) is provided with a sliding block (41) that slides in cooperation with the groove (42) to adjust the distance between the two cover plates (2).

5. The sample homogenizer for visual detection of dragon fruit virus according to claim 4, characterized in that, The bag clamping mechanism (4) includes a compression plate (43) disposed on one of the cover plates (2) and a slot (44) disposed on the other cover plate (2) for cooperating with the compression plate (43) to clamp the disposable homogenizing bag.

6. The sample homogenizer for visual detection of dragon fruit virus according to claim 5, characterized in that, The bag clamping mechanism (4) also includes a buckle fastener (45) and a pipe clamp (46) respectively disposed on the two cover plates (2), wherein the buckle fastener (45) and the pipe clamp (46) engage with each other to lock the two cover plates (2).

7. The sample homogenizer for visual detection of dragon fruit virus according to claim 1, characterized in that, The homogenizer (1) has a transparent observation window (37) on its body.

8. The sample homogenizer for visual detection of dragon fruit virus according to claim 1, characterized in that, The device also includes an operating console (38), which is electrically connected to the electric telescopic rod (31) and is used to control the start, stop, extension frequency or stroke of the electric telescopic rod (31).

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