A sample positioning and clamping device for SPR detection

Abstract

The utility model relates to biological sensing technical field discloses a kind of sample positioning clamping devices for SPR detection, device includes shell, cover pressure subassembly and side pressure subassembly.The cover pressure subassembly includes mounting plate, first slide and first fixed plate, using first main magnet and second main magnet same pole repulsion drive first fixed plate to realize transverse clamping, using first lower magnet and second lower magnet opposite pole attraction drive mounting plate to realize longitudinal compression;The side pressure subassembly uses first side magnet and second side magnet same pole repulsion drive side pressing plate to realize the clamping of another direction, three-dimensional automatic positioning to chip is completed jointly.Gasket is equipped with detachable injection port and suction port sealing connection device.The utility model solves the problem of uneven pressure of traditional bolt fixation by magnetic drive, realizes uniform, stable and repeatable clamping and sealing, compact structure, efficient and convenient operation.

Description

Technical Field

[0001] This utility model relates to the field of biosensing technology, and in particular to a sample positioning and clamping device for SPR detection. Background Technology

[0002] Surface plasmon resonance (SPR) technology, as a highly sensitive, real-time, label-free method for detecting biomolecular interactions, is widely used in life sciences, drug screening, disease diagnosis, and environmental monitoring. The core of SPR technology is utilizing the plasmon resonance effect on the surface of noble metal thin films to detect the binding or dissociation of biomolecules on the chip surface by detecting changes in refractive index. To achieve this detection process, the sample to be tested typically needs to flow in liquid form through the surface of an SPR chip to which specific probes are fixed. Therefore, accurately positioning and stably clamping the SPR chip within the detection device, and constructing a sealed, leak-free fluid channel, are crucial aspects of SPR detection device design.

[0003] Existing SPR testing devices typically use mechanical bolts, clips, or clamps to secure the chips. However, these traditional mechanical methods have several problems. For example, when using bolts, the force applied by the operator when tightening the bolts is often inconsistent, leading to uneven pressure on different areas of the chip. This uneven pressure can not only cause stress on the SPR chip, affecting the stability and accuracy of the detection signal, but more importantly, it can cause uneven pressure on the sealing ring on the chip surface at different locations, preventing the formation of a complete and uniform sealing channel. Once the channel is not sealed properly, the liquid sample to be tested can leak or even cross-contaminate, severely contaminating the equipment and directly causing test failure. In addition, the repeated tightening and loosening of bolts increases equipment wear, reduces its lifespan, and, in scenarios requiring rapid chip replacement for batch testing, the cumbersome fixing process significantly reduces work efficiency. In some cases, to avoid the problems caused by bolt fixing, more complex hydraulic or pneumatic clamps may be used, but these solutions often increase the size, weight, and cost of the equipment, and have higher requirements for the operating environment, hindering miniaturization and widespread application. Therefore, this utility model proposes a sample positioning and clamping device for SPR testing to overcome the shortcomings of the prior art. Utility Model Content

[0004] To overcome the above deficiencies, this utility model provides a sample positioning and clamping device for SPR detection, which aims to improve the problems in the prior art where the sample positioning and clamping device for SPR detection uses traditional mechanical methods such as bolt fixing to fix the SPR detection chip, resulting in uneven clamping pressure, which leads to liquid leakage or affects the stability of the detection signal, as well as low operating efficiency and inconvenient assembly.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: A sample positioning and clamping device for SPR testing includes a housing, a base plate disposed inside the housing, a cover connected to the housing, a cover pressure assembly, and a side pressure assembly.

[0006] The cover assembly includes a mounting plate, on which a first sliding rod is provided facing each other along a first direction, and two first fixing plates are respectively slidably mounted on the first sliding rod; each first fixing plate is provided with a first main magnet, and a second main magnet with the same pole opposite to the first main magnet is provided on the housing; a first lower magnet is provided at the bottom of the mounting plate, and a second lower magnet with the opposite pole opposite to the first lower magnet is provided at the bottom of the housing.

[0007] Furthermore, the side pressure assembly includes a second slide bar arranged opposite to each other along the second direction, and two side pressure plates are slidably arranged on the second slide bar respectively; each side pressure plate is provided with a first side magnet, and the housing is provided with a second side magnet with the same pole opposite to the first side magnet.

[0008] The housing, cover pressure assembly, side pressure assembly, base plate, and cover are combined in a specific manner: the first fixing plate of the cover pressure assembly laterally clamps the SPR detection chip through sliding engagement with the first slide rod, and its first main magnet and the second main magnet on the housing generate repulsive force with the same poles, ensuring uniform clamping force; the mounting plate of the cover pressure assembly attracts the opposite poles of the first lower magnet at its bottom and the second lower magnet at the bottom of the housing, causing the mounting plate to drive the first fixing plate to press the SPR detection chip vertically; the side pressure plate of the side pressure assembly laterally clamps the SPR detection chip in another direction through sliding engagement with the second slide rod, and its first side magnet and the second side magnet on the housing generate repulsive force with the same poles.

[0009] Preferably, the cover is pivotally connected to the housing via a connecting shaft; the cover is provided with an upper pin, and the housing is provided with a lower pin that engages with the upper pin.

[0010] Preferably, the cover has a placement groove; the extraction port sealing connection device and the injection port sealing connection device are detachably installed in the placement groove.

[0011] Preferably, the injection port sealing connection device and the extraction port sealing connection device have the same structure, which includes an interface, a connecting pipe communicating with the interface, and a rubber sealing ring disposed at the bottom end of the connecting pipe; a filter screen is disposed inside the connecting pipe.

[0012] Preferably, the outer wall of the injection port sealing connection device is provided with a groove, a limiting ring, and a rubber column located between the groove and the limiting ring.

[0013] Preferably, both ends of the first slide rod are fixed to the mounting plate.

[0014] Preferably, the two ends of the second slide rod are fixed to the inner wall of the housing.

[0015] Preferably, the sample positioning and clamping device for SPR testing further includes a housing, which is fixed to the outside of the shell, and the second lower magnet is disposed inside the housing.

[0016] This utility model has the following beneficial effects: 1. In this utility model, by setting multiple sets of like-pole repulsive magnets in the cover pressure assembly and the side pressure assembly to drive the first fixing plate and the side pressure plate to move in opposite directions along the slide bar, and at the same time using the opposite-pole attracting lower magnet to drive the mounting plate to press down as a whole, the technical solution solves the problem in the prior art that when using bolts to fix SPR chips, the clamping pressure is uneven due to the different manual operation force, which affects the stability of the test results. It achieves the effect of uniform, automatic and repeatable clamping and fixing of the chip in three dimensions.

[0017] 2. In this utility model, by opening a placement groove on the cover and detachably installing an injection port sealing connection device and an extraction port sealing connection device with a rubber sealing ring in the groove, the technical solution solves the problems of cumbersome operation, easy leakage and difficult maintenance when establishing a sealed flow path for SPR chips in the prior art. It achieves the technical effect of quickly constructing a modular, highly sealed detection fluid channel and facilitating subsequent cleaning and replacement.

[0018] 3. In this utility model, by integrating the magnetically driven clamping mechanism and the modular fluid connection device into a housing with a cover and a locking pin, the technical solution solves the problems of the existing SPR detection device having a dispersed structure, numerous components, and complex operation process. It achieves the technical effects of simplifying the equipment structure, improving the efficiency of sample clamping and flow path construction, and enhancing the overall compactness and ease of use of the device. Attached Figure Description

[0019] Figure 1 This is a three-dimensional schematic diagram of a sample positioning and clamping device for SPR testing proposed in this utility model; Figure 2 This is a schematic diagram of the cover of a sample positioning and clamping device for SPR testing proposed in this utility model; Figure 3 This is a schematic diagram of the pressure cap assembly of a sample positioning and clamping device for SPR testing proposed in this utility model; Figure 4 This is a cross-sectional schematic diagram of the outer shell of a sample positioning and clamping device for SPR testing proposed in this utility model. Figure 5This is a schematic diagram of the injection port sealing connection device of the sample positioning and clamping device for SPR testing proposed in this utility model.

[0020] Legend: 1. Housing; 2. Outer shell; 3. Cover assembly; 301. First fixing plate; 302. First main magnet; 303. Second main magnet; 304. Mounting plate; 305. First slide rod; 306. First lower magnet; 307. Second lower magnet; 4. Extraction port sealing connection device; 5. Injection port sealing connection device; 501. Interface; 502. Connecting pipe; 503. Slide groove; 504. Rubber column; 505. Limiting ring; 506. Rubber sealing ring; 6. Side pressure assembly; 601. Side pressure plate; 602. First side magnet; 603. Second side magnet; 604. Second slide rod; 7. Upper pin; 8. Lower pin; 9. Placement groove; 10. Base plate; 11. Cover; 12. Connecting shaft. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0022] Reference Figures 1-5 This utility model provides a sample positioning and clamping device for SPR detection, which aims to solve the problem of uneven pressure affecting the detection results when using bolts to fix SPR detection chips in the prior art. The sample positioning and clamping device for SPR detection includes a housing 1, a base plate 10 fixedly connected inside the housing 1, and a cover 11 rotatably connected to the housing 1 via a connecting shaft 12.

[0023] The sample positioning and clamping device for SPR testing further includes a cover pressure assembly 3 and a side pressure assembly 6 disposed inside the housing 1, and an outer shell 2 is fixedly connected to the outside of the housing 1.

[0024] Specifically, the cover assembly 3 includes a mounting plate 304, on which a first sliding rod 305 is fixedly connected along a first direction and is slidably connected to the first sliding rod 305. Each first fixing plate 301 is fixedly provided with a first main magnet 302, and a second main magnet 303 is fixedly provided on the inner wall of the housing 1. The magnetic poles of each first main magnet 302 and the second main magnet 303 opposite to it are arranged with the same poles. A first lower magnet 306 is fixedly provided at the bottom of the mounting plate 304, and a second lower magnet 307 is fixedly provided inside the housing 2. The magnetic poles of the first lower magnet 306 and the second lower magnet 307 are arranged with opposite poles.

[0025] The side pressure assembly 6 includes a second slide rod 604 arranged opposite each other along a second direction, which is perpendicular to the aforementioned first direction. The two ends of the second slide rod 604 are fixedly connected to the inner wall of the housing 1. Two side pressure plates 601 are slidably connected to the second slide rod 604 respectively. A first side magnet 602 is fixedly arranged on each side pressure plate 601. A second side magnet 603 is fixedly arranged on the inner wall of the housing 1. The magnetic poles of each first side magnet 602 and the second side magnet 603 opposite to it are arranged with the same poles opposite each other.

[0026] In a preferred embodiment, the cover 11 is provided with an upper pin 7, and the housing 1 is provided with a lower pin 8 that is inserted and engaged with the upper pin 7.

[0027] As another preferred embodiment, the upper surface of the cover 11 is provided with a placement groove 9, and the extraction port sealing connection device 4 and the injection port sealing connection device 5 can be detachably installed inside the placement groove 9.

[0028] Furthermore, the outlet sealing connection device 4 and the injection outlet sealing connection device 5 have the same structure, which includes an interface 501 set at the top, a connecting pipe 502 integrally connected to the lower end of the interface 501, a filter screen fixedly installed inside the connecting pipe 502, and a rubber sealing ring 506 fixedly connected to the bottom end of the connecting pipe 502.

[0029] Furthermore, a groove 503 is machined on the outer wall of the injection port sealing connection device 5, and a rubber column 504 and a limiting ring 505 are also fitted on its outer wall, with the rubber column 504 located between the groove 503 and the limiting ring 505.

[0030] In the cover assembly 3, the two ends of the first slide bar 305 are fixedly connected to the mounting plate 304.

[0031] In the side pressure assembly 6, the two ends of the second slide rod 604 are fixedly connected to the inner wall of the housing 1.

[0032] Working principle: During operation, the SPR detection chip is first placed on the base plate 10 inside the housing 1, and then fixed by the cover pressing assembly 3. Due to the magnetic force generated by the mutual repulsion between the second main magnet 303 and the first main magnet 302, the first fixing plates 301 on both sides move in opposite directions along the first slide bar 305, thereby applying uniform pressure to both sides of the chip. At the same time, due to the mutual attraction between the second lower magnet 307 inside the housing 2 and the first lower magnet 306 on the mounting plate 304, the mounting plate 304 will drive the first fixing plate 301 to move downward as a whole, thereby pressing and fixing the chip towards the base plate 10. Subsequently, it is further fixed by the side pressing assembly 6, through the first side magnet The magnetic force generated by the mutual repulsion between the body 602 and the second side magnet 603 causes the two side pressure plates 601 to move in opposite directions along the second slide bar 604, thereby fixing the other two sides of the chip. After the chip is clamped in four directions and pressed longitudinally, the cover 11 connected to the housing 1 via the connecting shaft 12 is closed, and the upper pin 7 is inserted into the lower pin 8 to lock it. Finally, the extraction port sealing connection device 4 and the injection port sealing connection device 5 are installed into the placement groove 9 respectively. The rubber sealing ring 506 at the bottom of the device contacts the chip surface to form a sealed flow path. The external conduit with the sample to be tested is connected to the interface 501, and the sample liquid can flow through the filter screen inside the connecting tube 502 and be injected into the detection area on the chip surface.

[0033] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A sample positioning and clamping device for SPR testing, comprising: Shell (1); The bottom plate (10) is disposed inside the housing (1). as well as Cover (11) connected to the housing (1); Its characteristic is that it further includes: A cover pressing assembly (3) includes a mounting plate (304), on which a first sliding rod (305) is provided facing each other along a first direction, and two first fixing plates (301) are respectively slidably disposed on the first sliding rod (305); each of the first fixing plates (301) is provided with a first main magnet (302), and the housing (1) is provided with a second main magnet (303) corresponding to the first main magnet (302); a first lower magnet (306) is provided at the bottom of the mounting plate (304), and a second lower magnet (307) corresponding to the first lower magnet (306) is provided at the bottom of the housing (1); and The side pressure assembly (6) includes a second slide rod (604) arranged opposite each other along the second direction, and two side pressure plates (601) are slidably arranged on the second slide rod (604); each side pressure plate (601) is provided with a first side magnet (602), and the housing (1) is provided with a second side magnet (603) that is opposite to the first side magnet (602) with the same pole.

2. The sample positioning and clamping device for SPR testing according to claim 1, characterized in that: The cover (11) is pivotally connected to the housing (1) via a connecting shaft (12); the cover (11) is provided with an upper pin (7), and the housing (1) is provided with a lower pin (8) that engages with the upper pin (7).

3. The sample positioning and clamping device for SPR testing according to claim 1, characterized in that: The cover (11) has a placement groove (9); the extraction port sealing connection device (4) and the injection port sealing connection device (5) are detachably installed in the placement groove (9).

4. The sample positioning and clamping device for SPR testing according to claim 3, characterized in that: The injection port sealing connection device (5) and the extraction port sealing connection device (4) have the same structure, including an interface (501), a connecting pipe (502) communicating with the interface (501), and a rubber sealing ring (506) provided at the bottom end of the connecting pipe (502); a filter screen is provided inside the connecting pipe (502).

5. The sample positioning and clamping device for SPR testing according to claim 4, characterized in that: The outer wall of the injection port sealing connection device (5) is provided with a groove (503), a limiting ring (505), and a rubber column (504) located between the groove (503) and the limiting ring (505).

6. The sample positioning and clamping device for SPR testing according to claim 1, characterized in that: The two ends of the first slide bar (305) are fixed on the mounting plate (304).

7. The sample positioning and clamping device for SPR testing according to claim 1, characterized in that: The two ends of the second slide rod (604) are fixed to the inner wall of the housing (1).

8. The sample positioning and clamping device for SPR testing according to claim 1, characterized in that: It also includes a housing (2) which is fixed to the outside of the housing (1), and the second lower magnet (307) is disposed inside the housing (2).

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