Amino acid molecule detection device and detection array chip

Abstract

The utility model relates to biological detection technical field discloses an amino acid molecule detection device and detection array chip, this amino acid molecule detection device includes: ultraviolet light source, detection array chip and current collection circuit, the detection array chip includes switch layer, lower electrode layer, amorphous gallium oxide layer and upper electrode layer in proper order, the switch in switch layer with lower electrode in lower electrode layer one -to -one, the ultraviolet light that ultraviolet light source sent reaches the detection array chip after the amino acid molecule sample solution of waiting for detecting, the detection array chip exports photoelectric current, the current collection circuit gathers the photoelectric current that the detection array chip exports, and the photoelectric current is used for analysis to obtain the concentration and / or kind of amino acid molecule. The utility model can realize convenient and fast and low -cost amino acid molecule real -time detection.

Description

Technical Field

[0001] This utility model relates to the field of biological detection technology, specifically to an amino acid molecular detection device and detection array chip. Background Technology

[0002] Amino acids are ubiquitous in various proteins and play a crucial regulatory role in cellular function. Detecting the concentration of amino acids and their derivatives is essential for understanding their biological functions and diagnosing related diseases. However, traditional detection methods such as high-performance liquid chromatography (HPLC), electro-fogging detectors, and spectrometry are highly dependent on derivatization and involve expensive instruments. HPLC instruments are expensive and bulky, making rapid and direct detection of amino acid molecules anytime and anywhere difficult, significantly increasing transportation costs and hindering real-time detection. Therefore, there is an urgent need for low-cost amino acid detection methods that enable real-time detection. Utility Model Content

[0003] This invention provides an amino acid molecule detection device and a detection array chip to solve the current problem of difficulty in achieving convenient, fast, real-time and low-cost amino acid molecule detection.

[0004] In a first aspect, this utility model provides an amino acid molecule detection device, which includes: an ultraviolet light source, a detection array chip, and a current acquisition circuit; The detection array chip comprises, in sequence, a switch layer, a lower electrode layer, an amorphous gallium oxide layer, and an upper electrode layer; the switches in the switch layer correspond one-to-one with the lower electrodes in the lower electrode layer. The ultraviolet light emitted by the ultraviolet light source passes through the amino acid molecule sample solution to be detected and reaches the detection array chip. The detection array chip outputs a photocurrent, and the current acquisition circuit acquires the photocurrent output by the detection array chip. The photocurrent is used to analyze and obtain the concentration and / or type of amino acid molecules.

[0005] In one optional embodiment, the amino acid molecule detection device further includes a light-transmitting container, which includes at least two accommodating spaces, one of which is used to accommodate a solvent that is the same as the solvent of the amino acid molecule sample solution, and the other accommodating spaces are used to accommodate the amino acid molecule sample solution.

[0006] In one alternative embodiment, the light-transmitting container is a polydimethylsiloxane container or a quartz cuvette.

[0007] In one optional embodiment, the amino acid molecule detection device further includes an imaging module that generates an image based on the photocurrent output by the detection array chip.

[0008] In one alternative embodiment, the amino acid molecule detection device further includes an image display module for displaying an image generated based on the photocurrent.

[0009] In one alternative implementation, the switches in the switching layer are silicon-based metal-oxide-semiconductor field-effect transistor arrays or thin-film transistor arrays.

[0010] In one alternative embodiment, the thin-film transistor includes an amorphous silicon thin-film transistor, an oxide thin-film transistor, a low-temperature polycrystalline silicon oxide thin-film transistor, and an organic semiconductor thin-film transistor.

[0011] In one alternative embodiment, the thickness of the amorphous gallium oxide layer is 100 nanometers to 500 nanometers.

[0012] In one alternative embodiment, the thickness of the amorphous gallium oxide layer is 360 nanometers.

[0013] In one optional embodiment, the amino acid molecule detection device further includes a photocurrent analysis circuit connected to the current acquisition circuit, used to output the concentration and / or type of amino acid molecules based on the photocurrent output by the current acquisition circuit.

[0014] Secondly, this utility model provides an amino acid molecule detection array chip, which sequentially includes a switch layer, a lower electrode layer, an amorphous gallium oxide layer, and an upper electrode layer; the switches in the switch layer correspond one-to-one with the lower electrodes in the lower electrode layer.

[0015] This invention utilizes the principle of ultraviolet absorption by some amino acid molecules, combined with the advantages of amorphous gallium oxide's high sensitivity and flexible growth in the ultraviolet band, to provide an amino acid molecule detection device and an amino acid molecule detection array chip. This device detects the photocurrent corresponding to the sample solution of the amino acid molecule to be detected, and then the concentration and / or type of amino acid molecules can be obtained based on photocurrent analysis. The amino acid molecule detection device provided by this invention has advantages such as small weight, small size, low cost, portability, and simple operation. Moreover, compared with conventional detection methods, the derivatization and other pretreatment steps are eliminated when using the amino acid molecule detection device provided by this invention for amino acid molecule detection. Therefore, the amino acid molecule detection device provided by this invention can achieve convenient, fast, and low-cost real-time detection of amino acid molecules, and has broad application prospects for special occasions requiring temporary, rough, and rapid measurements. Attached Figure Description

[0016] To more clearly illustrate the specific embodiments of this utility model or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0017] Figure 1 This is a schematic block diagram of the structure of the amino acid molecule detection device according to an embodiment of the present invention; Figure 2 This is one of the structural schematic diagrams of the detection array chip according to an embodiment of the present utility model; Figure 3 This is a second schematic diagram of the structure of the detection array chip according to an embodiment of the present utility model; Figure 4 This is a schematic diagram of the structure of a polydimethylsiloxane container according to an embodiment of the present invention; Figure 5 This is an example graph showing the correspondence between the concentrations of four amino acids and the photocurrent ratio according to an embodiment of the present invention. Figure 6 This is a schematic diagram of a detection system using a single-point device according to an embodiment of the present invention; Figure 7 These are example images of detection images corresponding to four adenine molecule solutions with different molar concentrations according to embodiments of the present invention. Figure 8 These are example images of detection images corresponding to four different amino acid molecular solutions according to embodiments of this utility model. Detailed Implementation

[0018] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0019] It is understood that before using the technical solutions disclosed in the various embodiments of this utility model, users should be informed of the types, scope of use, and usage scenarios of the personal information involved in this utility model in an appropriate manner in accordance with relevant laws and regulations, and user authorization should be obtained.

[0020] In the description of this utility model, "multiple" means two or more, unless otherwise explicitly specified.

[0021] This invention provides an amino acid molecule detection device and a detection array chip, which enables convenient, fast, and low-cost amino acid molecule detection.

[0022] This embodiment provides an amino acid molecule detection device. Figure 1 This is a schematic diagram of the structure of the amino acid molecule detection device according to an embodiment of the present invention, as shown below. Figure 1 As shown, the amino acid molecule detection device includes: Ultraviolet light source 101, detection array chip 102 and current acquisition circuit 103; like Figure 2 and Figure 3 As shown, the detection array chip 102 sequentially includes a switch layer 11, a lower electrode layer 12, an amorphous gallium oxide (a-Ga2O3) layer 13, and an upper electrode layer 14; the switches in the switch layer 11 correspond one-to-one with the lower electrodes in the lower electrode layer; The ultraviolet light emitted by the ultraviolet light source passes through the amino acid molecule sample solution to be detected and reaches the detection array chip. The detection array chip outputs a photocurrent, which is then collected by the current acquisition circuit. This photocurrent is used to analyze and determine the concentration and / or type of the amino acid molecules. Specifically, since different concentrations and types of amino acid molecules have different absorption characteristics for ultraviolet light, the detection array chip can be used to detect the transmitted light containing information about the amino acid molecule sample solution, thereby enabling the detection and determination of information such as the type and / or concentration of amino acids.

[0023] Amino acid molecules with conjugated molecular structures can absorb ultraviolet light because their π electrons require only a small amount of energy to transition. Therefore, their absorption peaks can be detected in the near-ultraviolet end or near-ultraviolet region of the far-ultraviolet region. Thus, this invention provides an amino acid molecule detection device based on amorphous gallium oxide (a-Ga2O3) material. The detection array chip, including the amorphous gallium oxide (a-Ga2O3) layer, is an ultraviolet photodetector chip. Under ultraviolet light irradiation, the current is relatively large, while in darkness, the current is relatively small. Based on this, the detection array chip can be calibrated before using the amino acid molecule detection device provided in this embodiment of the invention for amino acid molecule detection. Specifically, the chip can be calibrated under ultraviolet light irradiation and in darkness. Under ultraviolet light irradiation, the current values ​​of each lower electrode in the chip are higher; in darkness, the current values ​​of each lower electrode are lower.

[0024] Specifically, amorphous gallium oxide (a-Ga2O3) material can be directly deposited on the switching layer using radio frequency magnetron sputtering technology. The thickness of the amorphous gallium oxide layer can be 100 nanometers to 500 nanometers, for example, 360 nanometers. The switching layer can include a switching matrix composed of multiple switches, which can suppress crosstalk signals.

[0025] This invention utilizes the principle of ultraviolet absorption by some amino acid molecules, combined with the advantages of amorphous gallium oxide's sensitivity and high growth flexibility in the ultraviolet band, to provide an amino acid molecule detection device. This device detects the photocurrent corresponding to the sample solution of the amino acid molecule to be detected, and then the concentration and / or type of the amino acid molecule can be obtained based on photocurrent analysis. The amino acid molecule detection device provided by this invention has advantages such as small weight, small size, low cost, portability, and simple operation. Moreover, compared with conventional detection methods, the derivatization and other pretreatment steps are eliminated when using the amino acid molecule detection device provided by this invention. Therefore, the amino acid molecule detection device provided by this invention can achieve convenient, fast, and low-cost real-time detection of amino acid molecules, and has broad application prospects for special occasions requiring temporary, rough, and rapid measurements.

[0026] In some alternative implementations, the switches in the switching layer are metal-oxide-semiconductor field-effect transistors (MOSFETs) or thin-film transistors (TFTs).

[0027] Thin-film transistors include amorphous silicon (a-Si) thin-film transistors, oxide thin-film transistors (related oxides represented by InGaZnO), low-temperature polycrystalline silicon (LTPS) thin-film transistors, and organic semiconductor thin-film transistors.

[0028] In some optional embodiments, the amino acid molecule detection device further includes a light-transmitting container comprising at least two accommodating spaces. One of the accommodating spaces is used to accommodate a solvent, which is the same solvent as the amino acid molecule sample solution and does not contain amino acid molecules or other materials. The other accommodating spaces are used to accommodate the amino acid molecule sample solution. The solvent for the amino acid molecule sample solution may be, for example, pure water or deionized water.

[0029] Specifically, one accommodating space corresponds to one or more lower electrodes. That is, by detecting the photocurrent of the lower electrode corresponding to the accommodating space, the photocurrent corresponding to the solution (solvent or amino acid molecule sample solution) contained in the accommodating space can be obtained.

[0030] The transparent container can be configured with multiple spaces, thereby enabling high-throughput detection of amino acid molecules, that is, the detection of multiple amino acid molecules of different concentrations and / or different types can be performed at the same time.

[0031] In some optional embodiments, the light-transmitting container is a polydimethylsiloxane (PDMS) container or a quartz cuvette.

[0032] The following example illustrates a method for detecting amino acid molecule concentration: First, the polydimethylsiloxane container 104 (e.g., with 4 accommodating spaces (also called small holes)) is placed... Figure 4 As shown, the sample is placed above the detection array chip 102. A solution of adenine molecules with a molar concentration of 0 (i.e., pure water) and three adenine molecules with different molar concentrations are added to the four accommodative spaces. Turning on the ultraviolet light source yields the photocurrent corresponding to each accommodative space, i.e., the photocurrent corresponding to each different solution.

[0033] Then, the ratio of the photocurrent corresponding to the three different molar concentrations of adenine molecules to the photocurrent corresponding to pure water was calculated (this can also be called the normalized photocurrent value).

[0034] Finally, based on the previously obtained relationship between the concentration of adenine molecules and the photocurrent ratio, the specific concentrations of these three adenine molecule solutions with different molar concentrations were obtained.

[0035] The relationship between the concentration of adenine molecules and the photocurrent ratio can be obtained in the following way: First, a polydimethylsiloxane container with multiple accommodating spaces (also called orifices) is placed above the detection array chip. Adenine molecular solutions with a molar concentration of 0 (i.e., pure water) and various adenine molecular solutions with different molar concentrations (known molar concentrations) are added to each of these spaces. Turning on the ultraviolet light source allows the photocurrent corresponding to each accommodating space, i.e., the photocurrent corresponding to each different solution, to be obtained.

[0036] Then, the ratio of the photocurrent corresponding to various adenine molecule solutions with different molar concentrations to the photocurrent corresponding to pure water is calculated, thus obtaining the relationship between the concentration of adenine molecules and the photocurrent ratio.

[0037] The relationship between the concentration of other amino acid molecules and the photocurrent ratio can be obtained using the same method. After experimentally obtaining the photocurrent ratios corresponding to the concentrations of various amino acid molecules, curve fitting and other methods can be used to obtain the corresponding curves between the concentrations of these amino acid molecules and the photocurrent ratio, such as... Figure 5 The curves showing the relationship between the concentrations of adenine, guanine, tyrosine, and phenylalanine molecules and the photocurrent ratio are shown.

[0038] To improve the accuracy of the correlation between amino acid molecule concentration and photocurrent ratio, single-point devices can be used to detect solutions of different types and concentrations of amino acid molecules. Specifically, detection systems using single-point devices include... Figure 6 As shown, the system includes an ultraviolet light source 101, a single-point device 604, an electrical connection circuit 603 (which may specifically include a detection probe and wires) for the single-point device 604 and an electrical signal acquisition unit 605, an electrical signal acquisition unit 605, and an analysis and control system 606. The container used to hold the amino acid molecular solution in this detection system can be a quartz cuvette 602.

[0039] In some optional embodiments, the amino acid molecule detection device further includes a photocurrent analysis circuit connected to the current acquisition circuit, used to output the concentration and / or type of amino acid molecules based on the photocurrent output by the current acquisition circuit. Specifically, the photocurrent analysis circuit may include a current conversion circuit, an analog divider, and a comparator. The current conversion circuit converts the photocurrent output by the current acquisition circuit into a voltage. The analog divider normalizes the voltage converted from the photocurrent, that is, divides the voltage corresponding to the amino acid molecule sample solution to be detected by the voltage corresponding to the solvent alone, to obtain a voltage ratio, which is equivalent to the photocurrent ratio. The comparator compares the voltage ratio with the standard photocurrent ratio corresponding to each concentration (or type) of amino acid molecule solution (the correspondence between the concentration (or type) of amino acid molecules and the photocurrent ratio is obtained in advance), thereby obtaining the concentration and / or type of amino acid molecules based on the comparison result.

[0040] In some optional embodiments, the amino acid molecule detection device further includes an imaging module that generates an image based on the photocurrent output by the detection array chip.

[0041] In this embodiment, the switch matrix in the switch layer defines the pixel size and suppresses crosstalk signals.

[0042] In some optional embodiments, the amino acid molecule detection device further includes an image display module for displaying an image generated based on the photocurrent.

[0043] Specifically, the image display module can be a computer monitor or a mobile phone screen.

[0044] For example, if a polydimethylsiloxane container with four cavities (also called orifices) is placed above a detection array chip, and a 0 mM adenine solution (i.e., pure water) and solutions with molar concentrations of 0.1 mM, 0.5 mM, and 1 mM adenine are added to each of the four cavities respectively, then by turning on the ultraviolet light source, the photocurrent corresponding to each cavity, i.e., the photocurrent corresponding to each different solution, can be obtained. Then, an image generated based on the photocurrent is shown below. Figure 7 As shown (the top left is the image area corresponding to a 1mM adenine molecule solution, the top right is the image area corresponding to a 0.1mM adenine molecule solution, the bottom left is the image area corresponding to a 0.5mM adenine molecule solution, and the bottom right is the image area corresponding to pure water).

[0045] from Figure 7 It can be seen that as the concentration of amino acid molecules increases, the absorption of ultraviolet light by the solution increases, resulting in a decrease in the amount of ultraviolet light reaching the chip below this area, and a decrease in the current value, which is reflected in the image as the circular hole becoming "darker".

[0046] Therefore, images can be used to qualitatively identify solutions of the same amino acid molecules at different concentrations. For example, solutions of the same amino acid molecules at different concentrations can be sorted according to their concentration. Furthermore, when multiple solutions of the same amino acid molecules exist, and the concentrations are known but cannot be distinguished, images can be used for differentiation and identification.

[0047] For example, if a polydimethylsiloxane container with four cavities (also called orifices) is placed above a detection array chip, and solutions of adenine, guanine, tyrosine, and phenylalanine, each with a molar concentration of 1 mM, are added to each of the four cavities, then by turning on the ultraviolet light source, the photocurrent corresponding to each cavity, i.e., the photocurrent corresponding to each different solution, can be obtained. Then, an image generated based on the photocurrent is shown below. Figure 8 As shown.

[0048] from Figure 8It can be seen that among the four amino acid solutions of the same concentration, adenine has the strongest absorption capacity for ultraviolet light, resulting in the weakest ultraviolet light and the lowest current value in the corresponding area of ​​the chip below, appearing as the "darkest" in the image. The current values ​​of the corresponding areas of the other three amino acid solutions are not significantly different, making them difficult to distinguish in the image, but they can still be differentiated by reading the current values ​​of the corresponding areas. Knowing that smaller current values ​​indicate higher absorbance, the current values ​​of a portion of the corresponding areas of each of the four amino acid solutions were selected and averaged to obtain the absorbance ranking: adenine (3×10⁻⁶). -10 A)> Guanine (1.8×10) -9 A)>Tyrosine (2.2×10) -9 A)> Phenylalanine (2.5×10 -9 A) The order of ultraviolet absorbance of the four amino acid molecules also matches the detection results of the single-point device.

[0049] Furthermore, qualitative analysis of amino acid molecules can be achieved based on the rate of change in absorbance caused by concentration variations. For example, adenine shows the fastest change in absorbance with concentration changes, while phenylalanine shows the slowest. Guanine's absorbance change is faster than that of tyrosine. Therefore, during the detection process, the concentrations of various amino acid molecules can be increased, and the types of amino acid molecules can then be qualitatively distinguished based on changes in the grayscale of the image.

[0050] Amino acid testing has a wide range of applications across various fields, with its core value lying in assessing biological metabolic status, optimizing product quality, and ensuring health and safety. Many metabolic diseases, such as phenylketonuria (PKU) and homocysteineuria (HCU), are related to metabolic disorders of specific amino acids. For example, in homocysteineuria, detecting homocysteine ​​levels can help diagnose cardiovascular disease risk. Furthermore, in food processing, monitoring the degradation or transformation of amino acids (such as the Maillard reaction) can control the formation of harmful substances (such as acrylamide) and ensure food safety.

[0051] Therefore, the amino acid molecular detection device and detection array chip provided in this embodiment of the present invention have significant application prospects in clinical disease diagnosis, diet control, ecological environment monitoring, and biomedical research and development.

[0052] In addition, this embodiment of the invention also provides an amino acid molecule detection array chip, which sequentially includes a switch layer, a lower electrode layer, an amorphous gallium oxide layer, and an upper electrode layer; the switches in the switch layer correspond one-to-one with the lower electrodes in the lower electrode layer. For details, please refer to the detection array chip in the above-described amino acid molecule detection device embodiment, which will not be repeated here.

[0053] Although embodiments of the present invention have been described in conjunction with the accompanying drawings, those skilled in the art can make various modifications and variations without departing from the spirit and scope of the present invention, and such modifications and variations all fall within the scope defined by the appended claims.

Claims

1. An amino acid molecule detection device, characterized in that, The amino acid molecule detection device includes: an ultraviolet light source, a detection array chip, and a current acquisition circuit; The detection array chip comprises, in sequence, a switch layer, a lower electrode layer, an amorphous gallium oxide layer, and an upper electrode layer; the switches in the switch layer correspond one-to-one with the lower electrodes in the lower electrode layer. The ultraviolet light emitted by the ultraviolet light source passes through the amino acid molecule sample solution to be detected and reaches the detection array chip. The detection array chip outputs a photocurrent, and the current acquisition circuit acquires the photocurrent output by the detection array chip. The photocurrent is used to analyze and obtain the concentration and / or type of amino acid molecules.

2. The amino acid molecule detection device according to claim 1, characterized in that, It also includes a light-transmitting container, which has at least two accommodating spaces, one of which is used to accommodate a solvent, the solvent being the same as that used in the amino acid molecule sample solution, and the other accommodating spaces are used to accommodate the amino acid molecule sample solution.

3. The amino acid molecule detection device according to claim 2, characterized in that, The light-transmitting container is a polydimethylsiloxane container or a quartz cuvette.

4. The amino acid molecule detection device according to claim 1, characterized in that, It also includes an imaging module that generates an image based on the photocurrent output by the detection array chip.

5. The amino acid molecule detection device according to claim 4, characterized in that, It also includes an image display module for displaying an image generated based on the photocurrent.

6. The amino acid molecule detection device according to claim 1, characterized in that, The switches in the switching layer are metal-oxide-semiconductor field-effect transistor arrays or thin-film transistor arrays.

7. The amino acid molecule detection device according to claim 6, characterized in that, Thin-film transistors include amorphous silicon thin-film transistors, oxide thin-film transistors, low-temperature polycrystalline silicon thin-film transistors, and organic semiconductor thin-film transistors.

8. The amino acid molecule detection device according to claim 1, characterized in that, The thickness of the amorphous gallium oxide layer is 100 nanometers to 500 nanometers.

9. The amino acid molecule detection device according to claim 1, characterized in that, It also includes a photocurrent analysis circuit, connected to the current acquisition circuit, for outputting the concentration and / or type of amino acid molecules based on the photocurrent output by the current acquisition circuit.

10. An amino acid molecule detection array chip, characterized in that, It comprises, in sequence, a switch layer, a lower electrode layer, an amorphous gallium oxide layer, and an upper electrode layer; the switches in the switch layer correspond one-to-one with the lower electrodes in the lower electrode layer.

Images