A high-efficiency medical electrophoresis separation device

Abstract

The utility model relates to a kind of high-efficiency medical electrophoresis separation device, including base, and base is placed with electrophoresis tank, and the rear of electrophoresis tank is fixed with stand, and first adjusting ring is fixed with the sleeve setting of stand, and the outer wall of first adjusting ring is fixed with the cantilevered rod of transverse, and cantilevered rod extends along front-back direction, and second adjusting ring is fixed with the sleeve setting of the sleeve setting of cantilevered rod, and the bottom of second adjusting ring is fixed with hook;It also includes separation cylinder, and the top of separation cylinder is equipped with ring, and the lower part of separation cylinder outer wall is provided with annular semi-permeable membrane layer, and annular semi-permeable membrane layer is communicated with separation cylinder inside, when separation cylinder is hung by hook with the ring, annular semi-permeable membrane layer is located in electrophoresis tank inside.The utility model is installed in the way of separation cylinder with the mode of hook and ring hanging, and personnel is convenient to take and place, and it is more convenient to use, the temperature of electrophoretic liquid is detected by temperature sensor, cold wind is blown to electrophoresis tank by radiating fan, electrophoretic liquid is air-cooled cooling, to improve electrophoresis separation efficiency.

Description

Technical Field

[0001] This utility model relates to the technical field of electrophoretic separation devices, specifically a high-efficiency medical electrophoretic separation device. Background Technology

[0002] The basic principle of electrophoresis for separating mixed proteins is based on the difference in protein charge, i.e., the difference in acidity or basicity. Under the influence of an external electric field, charged particles will move towards the electrode with the opposite charge; this phenomenon is called electrophoresis. Electrophoresis technology can be used for the analysis, separation, and preparation of biomolecules such as medical amino acids, peptides, proteins, and nucleotides, and is widely used in chemical engineering, medical detection, and other fields. However, current medical laboratory electrophoresis separation devices are complex in structure, large in size, and inconvenient to handle and adjust. Furthermore, during the electrophoresis process, the heat generated by the electrophoresis components is transferred to the electrophoresis buffer, causing the buffer to heat up and thus reducing the efficiency of electrophoresis separation. Utility Model Content

[0003] This invention addresses the shortcomings of existing technologies by providing a highly efficient medical electrophoresis separation device that is more convenient to use. During the electrophoresis process, the temperature can be adjusted according to the buffer solution temperature to ensure separation efficiency.

[0004] This utility model is achieved through the following technical solution: a high-efficiency medical electrophoresis separation device includes a base, on which an electrophoresis tank is placed. Positive and negative electrode rods are respectively provided on the left and right side walls of the electrophoresis tank, and the positive and negative electrode rods are connected to a power supply box via wires. A column is fixedly installed at the rear of the electrophoresis tank, and a first adjusting ring is fitted and fixed on the column. A transverse cantilever rod is fixedly connected to the outer wall of the first adjusting ring, extending in a front-rear direction. A second adjusting ring is fitted and fixed on the cantilever rod, and a hook is fixedly connected to the bottom of the second adjusting ring. The device also includes a separation cylinder, with a hanging ring at the top and an annular semi-permeable membrane layer at the lower part of the outer wall of the separation cylinder. The annular semi-permeable membrane layer communicates with the interior of the separation cylinder. When the separation cylinder is hung by the hanging ring and hook, the annular semi-permeable membrane layer is located inside the electrophoresis tank. A temperature sensor is installed at the center of the inner bottom of the electrophoresis tank.

[0005] This method involves adding electrophoresis solution to the electrophoresis tank, and supplying current to the positive and negative electrode rods on both sides via a power supply box to create an electric field in the electrophoresis solution. A temperature sensor detects the temperature of the electrophoresis solution. The experimental sample is placed in a separation cylinder, immersing it in the electrophoresis solution. Under the influence of the electric field, positive and negative particles in the sample pass through a ring-shaped semi-permeable membrane and flow towards the positive and negative electrodes, achieving electrophoretic separation of medical samples. The separation cylinder is installed using a hanging ring and hook connection, making it convenient for personnel to place and use, thus improving experimental efficiency.

[0006] As an optimization, the column has multiple first through holes evenly distributed along the axial direction, and a first adjusting ring is fitted with a first pin that mates with the first through holes. In this optimized solution, the first adjusting ring is detachably fixed to the column by the first pin, which facilitates vertical adjustment of its position, thereby making it easier to adjust the different heights of the separating cylinder and further improving convenience.

[0007] As an optimization, the cantilever rod has multiple second through holes evenly distributed along its axial direction, and a second adjusting ring is fitted with a second pin that mates with the second through holes. In this optimized solution, the second adjusting ring is detachably fixed to the cantilever rod via the second pin, facilitating adjustment of the lateral position of the separating cylinder and further improving convenience.

[0008] As an optimization, the separation cylinder includes a cylinder body, a top cover, and a bottom cover. The cylinder body has openings at both the top and bottom. The top cover is connected and fixed to the upper end of the cylinder body via a fastener. A hanging ring is fixed to the top surface of the top cover. The lower end of the cylinder body is fixed to the bottom cover via multiple connecting rods. These connecting rods are evenly distributed along the circumference of the cylinder body, and the annular semi-permeable membrane layer covers the outside of the connecting rods. This optimized separation cylinder adopts a separate design for the cylinder body and top cover, which are detachably fixed via fasteners, facilitating sample placement inside the cylinder. The cylinder body and bottom cover are connected by connecting rods to form a through-hole that allows particles to pass through, enabling communication between the annular semi-permeable membrane layer and the interior of the cylinder body, thus facilitating particle permeation.

[0009] As an optimization, drain pipes are provided at the bottom of both the left and right side walls of the electrophoresis tank, and valves are installed on the drain pipes. This optimization facilitates the discharge of the separated electrophoretic solution.

[0010] As an optimization, the power supply box is an adjustable DC power supply box, which is connected to an AC power source through a rectifier and a transformer. This optimized solution maintains stable current and voltage.

[0011] As an optimization, a heat dissipation groove is formed at the center of the top surface of the base. Several cooling fans are installed in the heat dissipation groove, and the power cords of the cooling fans are connected to the power supply box. Multiple support rods are evenly distributed and fixed in the heat dissipation groove along the axial direction. The support rods are arranged horizontally and are located above the cooling fans. This optimized solution uses cooling fans to blow cool air into the electrophoresis tank, thereby cooling the electrophoretic solution and preventing excessive temperature, thus ensuring the efficiency of electrophoretic separation.

[0012] As an optimization, the support rod has a triangular structure, with one end of the triangular support rod pointing downwards, and the upper surface of the support rod is flush with the top surface of the base. This optimized design guides the airflow of the cooling fan, causing the airflow to converge towards the center, thereby increasing the airflow intensity and improving the heat dissipation effect.

[0013] As an optimization, the bottom of the heat dissipation tank has a heat dissipation through-hole located below the cooling fan, and a filter screen is installed inside the heat dissipation through-hole. This optimized solution dissipates heat from the cooling fan through the heat dissipation through-hole and filters impurities through the filter screen to prevent external impurities from entering.

[0014] The beneficial effects of this invention are as follows: Electrophoresis solution is added to the electrophoresis tank, and current is supplied to the positive and negative electrode rods on both sides via a power supply box, creating an electric field in the electrophoresis solution. Experimental samples are placed in a separation cylinder, immersing them in the electrophoresis solution. Under the influence of the electric field, positive and negative particles in the sample flow through the annular semi-permeable membrane to separate towards the positive and negative electrodes, achieving electrophoretic separation of medical samples. Because the separation cylinder is installed using a hanging ring and hook connection, it is convenient for personnel to handle, making it more convenient to use and improving experimental efficiency. The first adjusting ring can slide up and down along the column for positioning, and the second adjusting ring can slide back and forth along the cantilever rod for positioning, thereby facilitating the adjustment of the height and horizontal position of the separating cylinder and further improving ease of use; By installing a temperature sensor inside the electrophoresis tank, the temperature of the electrophoretic solution can be easily detected. A cooling fan blows cold air into the electrophoresis tank to cool the electrophoretic solution and prevent it from getting too hot, thus ensuring the efficiency of electrophoretic separation. Attached Figure Description

[0015] Figure 1 This is a front view of the present utility model; Figure 2 This is a side view of the present invention; Figure 3 This is a cross-sectional view of the separation cylinder; Figure 4 This is a sectional view of the base; As shown in the figure: 1. Base, 2. Electrophoresis tank, 3. Positive electrode rod, 4. Negative electrode rod, 5. Drain pipe, 6. Temperature sensor, 7. Power supply box, 8. Rectifier, 9. Transformer, 10. Separation cylinder, 101. Cylinder body, 102. Top cover, 103. Bottom cover, 104. Connecting rod, 105. Annular semi-permeable membrane layer, 106. Fastener, 11. Column, 12. First adjusting ring, 13. Cantilever rod, 14. Second adjusting ring, 15. First pin, 16. Second pin, 17. Hook, 18. Hanging ring, 19. First perforation, 20. Second perforation, 21. Heat dissipation sink, 22. Heat dissipation fan, 23. Support rod, 24. Heat dissipation through hole, 25. Dustproof mesh cover. Detailed Implementation

[0016] To clearly illustrate the technical features of this solution, the following detailed implementation method will be used to describe the solution.

[0017] like Figures 1-4As shown, a high-efficiency medical electrophoresis separation device includes a base 1, on which an electrophoresis tank 2 is placed. Positive electrode rods 3 and negative electrode rods 4 are respectively provided on the left and right side walls of the electrophoresis tank 2, and the positive electrode rods 3 and negative electrode rods 4 are respectively connected to a power supply box 7 through wires.

[0018] In this embodiment, the electrophoresis tank 2 is a rectangular tank made of transparent acrylic sheet. This serves two purposes: firstly, it provides insulation, and secondly, it allows personnel to easily observe the internal electrophoresis process and record data. The power supply box 7 is an adjustable DC power supply box. The positive electrode rod 3 is connected to the positive terminal of the power supply box 7 via a wire, and the negative electrode rod 4 is connected to the negative terminal of the power supply box 7 via a guide. The power supply box 7 is connected to an AC power source through a rectifier 8 and a transformer 9 to maintain stable current and voltage.

[0019] In this embodiment, drain pipes 5 are provided at the bottom of both the left and right side walls of the electrophoresis tank 2. Valves are installed on the drain pipes 5 to facilitate the discharge of the separated electrophoretic solution.

[0020] A column 11 is fixedly installed at the rear of the electrophoresis tank 2. In this embodiment, the bottom of the column 11 is fixedly connected to the base 1. A first adjusting ring 12 is sleeved and fixed on the column 11, and a transverse cantilever rod 13 is fixedly connected to the outer wall of the first adjusting ring 12. The cantilever rod 13 extends in the front-back direction, and a second adjusting ring 14 is sleeved and fixed on the cantilever rod 13. A hook 17 is fixedly connected to the bottom of the second adjusting ring 14. The system also includes a separation cylinder 10. A hanging ring 18 is provided at the top of the separation cylinder 10, and an annular semi-permeable membrane layer 105 is provided at the lower part of the outer wall of the separation cylinder 10. The annular semi-permeable membrane layer 105 communicates with the interior of the separation cylinder 10. When the separation cylinder 10 is hung by the hanging ring 18 and the hook 17, the annular semi-permeable membrane layer 105 is located inside the electrophoresis tank 2.

[0021] Specifically, the column 11 has multiple first through holes 19 evenly distributed along its axial direction, and a first adjusting ring 12 is provided with a first pin 15 that mates with the first through holes 19. The first adjusting ring 12 is detachably fixed to the column 11 by the first pin 15, facilitating vertical position adjustment and thus allowing for adjustment of different heights of the separating cylinder 10, further improving convenience. The cantilever rod 13 has multiple second through holes 20 evenly distributed along its axial direction, and a second adjusting ring 14 is provided with a second pin 16 that mates with the second through holes 20. The second adjusting ring 14 is detachably fixed to the cantilever rod 13 by the second pin 16, facilitating adjustment of the lateral position of the separating cylinder 10, further improving convenience. The first adjusting ring 12 can slide vertically along the column 11, and the second adjusting ring 14 can slide back and forth along the cantilever rod 13, thus facilitating adjustment of the height and horizontal position of the separating cylinder 10, improving ease of use.

[0022] The separating cylinder 10 includes a cylinder body 101, a top cover 102, and a bottom cover 103. The cylinder body 101 has openings at both the top and bottom. The top cover 102 is connected and fixed to the upper end of the cylinder body 101 via fasteners 106, and a hanging ring 18 is fixed to the top surface of the top cover 102. In this embodiment, the top cover 102 and the cylinder body 101 are connected by two fasteners 106, which are symmetrically distributed on the left and right sides of the cylinder body. Each fastener 106 includes a buckle and a hook. The buckle is fixed to the upper end of the cylinder body, and the hook is fixed to the top cover. The detachable connection between the cylinder body and the top cover is achieved through the hook and hook connection. The fasteners can be directly purchased.

[0023] The lower end of the cylinder 101 is fixedly connected to the bottom cover 103 by multiple connecting rods 104. The multiple connecting rods 104 are evenly distributed around the circumference of the cylinder 101, and the annular semi-permeable membrane layer 105 covers the outside of the multiple connecting rods 104. The upper end of the annular semi-permeable membrane layer 105 is sealed and fixedly connected to the bottom of the cylinder 101, and the lower end of the annular semi-permeable membrane layer 105 is sealed and fixedly connected to the bottom cover 103. The inner wall of the annular semi-permeable membrane layer 105 is fixedly connected to the circumferential connecting rods 104.

[0024] The separation cylinder 10 adopts a separate design of cylinder body and top cover, which can be detached and fixed by fasteners, facilitating the placement of samples inside the cylinder. The cylinder body 101 and the bottom cover 103 are connected by multiple connecting rods 104 to form a through hole that allows particles to pass through, realizing communication between the annular semi-permeable membrane layer and the inside of the cylinder, facilitating particle permeation. The annular semi-permeable membrane layer is supported by multiple circumferential connecting rods to ensure its stability.

[0025] A temperature sensor 6 is installed at the center of the inner bottom of the electrophoresis tank 2, which facilitates the detection of the electrophoresis solution temperature. A heat dissipation groove 21 is formed at the center of the top surface of the base 1. Several cooling fans 22 are installed within the heat dissipation groove 21. The power cords of the cooling fans 22 are connected to the power supply box 7. The positive terminals of the cooling fans 22 and 7 are connected via wires, and their negative terminals are also connected via wires. A controller is also included. The temperature sensor 6 and the cooling fans 22 are all electrically connected to the controller. The temperature sensor transmits the temperature signal to the controller, which controls the start / stop and airflow of the cooling fans to achieve automatic temperature control, making it more convenient to use.

[0026] Multiple support rods 23 are evenly distributed and fixed along the axial direction within the heat dissipation tank 21. The support rods 23 extend to the left and right and are located above the cooling fan 22. The multiple support rods 23 are used to support the electrophoresis tank 2. The cooling fan 22 blows cold air into the electrophoresis tank 2 to cool the electrophoretic solution, preventing the temperature from getting too high and thus ensuring the electrophoretic separation efficiency.

[0027] The support rod 23 has a triangular structure, with one end of the triangular support rod 23 pointing downwards, and the upper surface of the support rod 23 is flush with the top surface of the base 1. The downward-pointing end of the support rod 23 guides the airflow of the cooling fan 22, causing the airflow to converge towards the center, thereby increasing the airflow intensity and improving the heat dissipation effect.

[0028] The bottom of the heat dissipation groove 21 has a heat dissipation through hole 24 located below the cooling fan 22, and a dustproof mesh cover 25 is installed inside the heat dissipation through hole 24. The cooling fan 22 is cooled through the heat dissipation through hole 24, and the dustproof mesh cover 25 filters impurities to prevent external impurities from entering.

[0029] Working Principle: During use, the electrophoresis tank 2 is placed on the support rod 23 of the heat dissipation tank 21, and the electrophoresis solution is added to the electrophoresis tank 2. Current is supplied to the positive electrode rods 3 and negative electrode rods 4 on both sides via the power supply box 7, creating an electric field in the electrophoresis solution. The experimental sample is placed in the separation cylinder 10, which is hung on a hook, immersing the sample in the electrophoresis solution. Under the influence of the electric field, positive and negative particles in the sample flow through the annular semi-permeable membrane layer 105 to separate towards the positive and negative electrodes, achieving electrophoretic separation of the medical sample. During the separation process, the temperature sensor 6 is used to detect the temperature of the electrophoresis solution, thereby controlling the cooling fan 22 to blow cold air into the electrophoresis tank 2 to cool the electrophoresis solution, preventing excessive temperature and ensuring efficient electrophoretic separation.

[0030] Of course, the above description is not limited to the examples above. Technical features of this utility model not described can be implemented by or using existing technology, and will not be repeated here. The above embodiments and drawings are only used to illustrate the technical solution of this utility model and are not intended to limit this utility model. This utility model has been described in detail with reference to preferred embodiments. Those skilled in the art should understand that any changes, modifications, additions or substitutions made by those skilled in the art within the scope of this utility model do not depart from the spirit of this utility model and should also fall within the protection scope of the claims of this utility model.

Claims

1. A high-efficiency medical electrophoresis separation device, comprising a base (1), an electrophoresis tank (2) placed on the base (1), positive electrode rods (3) and negative electrode rods (4) respectively provided on the left and right side walls of the electrophoresis tank (2), the positive electrode rods (3) and negative electrode rods (4) being connected to a power supply box (7) respectively via wires, characterized in that: A column (11) is fixed at the rear of the electrophoresis tank (2). A first adjusting ring (12) is fixed on the column (11). A horizontal cantilever rod (13) is fixed on the outer wall of the first adjusting ring (12). The cantilever rod (13) extends in the front-back direction. A second adjusting ring (14) is fixed on the cantilever rod (13). A hook (17) is fixed at the bottom of the second adjusting ring (14). It also includes a separation cylinder (10), with a hanging ring (18) on the top of the separation cylinder (10) and an annular semi-permeable membrane layer (105) on the lower part of the outer wall of the separation cylinder (10). The annular semi-permeable membrane layer (105) is connected to the inside of the separation cylinder (10). When the separation cylinder (10) is hung with the hook (17) through the hanging ring (18), the annular semi-permeable membrane layer (105) is located inside the electrophoresis tank (2). A temperature sensor (6) is provided at the center of the inner bottom of the electrophoresis tank (2).

2. The high-efficiency medical electrophoresis separation device according to claim 1, characterized in that: The column (11) has a plurality of first through holes (19) evenly distributed along the axial direction, and the first adjusting ring (12) is provided with a first pin (15) that cooperates with the first through holes (19).

3. The high-efficiency medical electrophoresis separation device according to claim 1, characterized in that: The cantilever rod (13) has multiple second through holes (20) evenly distributed along the axial direction, and the second adjusting ring (14) is provided with a second pin (16) that cooperates with the second through holes (20).

4. The high-efficiency medical electrophoresis separation device according to claim 1, characterized in that: The separation cylinder (10) includes a cylinder body (101), a top cover (102) and a bottom cover (103). The cylinder body (101) has openings at both the top and bottom ends. The top cover (102) is connected and fixed to the upper end of the cylinder body (101) by a fastener (106). The hanging ring (18) is fixed to the top surface of the top cover (102). The lower end of the cylinder body (101) is fixed to the bottom cover (103) by multiple connecting rods (104). The multiple connecting rods (104) are evenly distributed around the circumference of the cylinder body (101). The annular semi-permeable membrane layer (105) covers the outside of the multiple connecting rods (104).

5. The high-efficiency medical electrophoresis separation device according to claim 1, characterized in that: The bottom of the left and right side walls of the electrophoresis tank (2) is provided with a drain pipe (5), and a valve is installed on the drain pipe to facilitate the discharge of the separated electrophoretic liquid.

6. The high-efficiency medical electrophoresis separation device according to claim 1, characterized in that: The power supply box (7) is an adjustable DC power supply box, and the power supply box (7) is connected to the AC power supply through the rectifier (8) and the transformer (9).

7. The high-efficiency medical electrophoresis separation device according to claim 6, characterized in that: The base (1) has a heat dissipation groove (21) at the center of its top surface. Several cooling fans (22) are installed in the heat dissipation groove (21). The power cord of the cooling fan (22) is connected to the power supply box (7). Several support rods (23) are evenly distributed and fixed in the heat dissipation groove (21) along the axial direction. The support rods are arranged horizontally and are located above the cooling fan (22).

8. The high-efficiency medical electrophoresis separation device according to claim 7, characterized in that: The support rod (23) has a triangular structure, with one end of the triangular support rod (23) facing downwards, and the upper end of the support rod (23) is flush with the top surface of the base (1).

9. The high-efficiency medical electrophoresis separation device according to claim 7, characterized in that: The bottom of the heat dissipation sink (21) is provided with a heat dissipation through hole (24) located below the heat dissipation fan (22), and a dustproof mesh cover (25) is installed inside the heat dissipation through hole (24).

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