Magnetic nucleic acid gel electrophoresis apparatus

Abstract

The utility model discloses a kind of magnetic nucleic acid gel electrophoresis apparatus, including electrophoresis tank body, two electrode filaments, magnetic attraction connector female, electrophoresis apparatus upper cover, magnetic attraction connector male;In the utility model: magnetic attraction connector female and magnetic attraction connector male are connected by magnetic attraction and electricity, and the friction in the plugging process of magnetic attraction connector female and magnetic attraction connector male is basically eliminated, to avoid the wear of connector due to repeated unplugging, shaking, and further poor contact, the situation of power failure, prolong the service life of equipment interface;In the present application, electrode filament is set through electrophoresis tank body, then connect magnetic attraction connector female, magnetic attraction connector female is also installed in the magnetic attraction connector female mounting hole position of electrophoresis tank body side wall, so that the power cord of electrode filament connecting magnetic attraction connector female does not need to be routed in vertical direction and then routed in horizontal direction, and the power cord is not easy to break, reducing safety hazards.

Description

Technical Field

[0001] This utility model relates to the field of nucleic acid gel electrophoresis instrument technology, and in particular to a magnetic nucleic acid gel electrophoresis instrument. Background Technology

[0002] Nucleic acid gel electrophoresis is one of the most fundamental and crucial pieces of equipment in molecular biology laboratories, primarily used for the separation, analysis, and identification of nucleic acid (especially DNA and RNA) fragments. Nucleic acid molecules are amphoteric molecules; in electrophoresis buffers above their isoelectric point (such as pH 8.0), their bases do not dissociate, while their phosphate groups completely dissociate, giving the nucleic acid molecule a negative charge. Under the influence of an electric current, using agarose gel as a medium, nucleic acid molecules migrate from the negative electrode to the positive electrode. Because different DNA fragments differ in size and shape, their migration rates in an electric field vary. Simultaneously, dyes (such as EB or anthocyanin dyes) are added to the sample. These dyes form complexes with the DNA molecules, and after ultraviolet irradiation, the location of the DNA can be observed (by comparing it with a standard to determine its molecular weight), thus achieving the purpose of separation and identification.

[0003] When operating a current nucleic acid electrophoresis apparatus, a pre-prepared agarose gel with neatly arranged wells needs to be placed into the apparatus. Then, electrode buffer is added to the apparatus, the apparatus is covered, and a voltage of about 80-120V is applied. Under the influence of the current, nucleic acid molecules will move from the negative electrode to the positive electrode, ultimately achieving the purpose of separating nucleic acid fragments.

[0004] Older nucleic acid gel electrophoresis instruments suffer damage in various locations after a period of use. This is mainly due to the long-term perfusion of the instrument with electrode buffer solution, a salt solution. The instrument is in a high-salt, high-humidity environment for extended periods, causing it to yellow and become brittle. In addition, the structural design flaws of older instruments lead to high maintenance costs, delays in research progress, and inconvenience due to insufficient consideration of usage scenarios. The main issues are as follows: The power cord is installed perpendicularly to the top cover of the electrophoresis apparatus, causing the power cord to be in a 90-degree bend for a long time, which makes it easy to break and poses a safety hazard. The power cord fixing interface on the top cover of the electrophoresis apparatus is always under stress and in a high-salt environment, making it prone to cracking. Electrode connectors are in constant contact with the electrode buffer solution and require repeated insertion and removal, making them prone to shaking and breakage. If the electrode connector breaks, the entire electrode wire and support need to be replaced. The electrode wire is made of pure platinum, which is very expensive. After a period of use, the top cover of the electrophoresis apparatus becomes worn due to repeated plugging and unplugging, causing it to wobble and resulting in poor contact and inability to conduct electricity. The cover of the electrophoresis apparatus cannot be opened with one hand, which is inconvenient during the experiment; Existing electrophoresis apparatuses do not consider scenarios where electrode buffer solutions need to be recovered, and lack corresponding auxiliary structures. As a result, electrode solutions often spill out during the recovery process, leading to waste.

[0005] Causes: The power cord of the existing nucleic acid gel electrophoresis instrument is vertically fixed on the top cover of the instrument, with the other end plugged into the power supply during use. This results in the power cord being bent at a 90-degree angle regardless of whether it is in use or not, and the connection between the power cord and the electrophoresis instrument is always under stress, making it very easy to crack, leading to leakage and creating a safety hazard.

[0006] Nucleic acid gel electrophoresis apparatus relies on electrode buffers to distribute the electric field. Many critical components need to be immersed in the electrode buffer for extended periods, and this electrode buffer is composed of various salts. This results in multiple components... After a period of use, it becomes brittle and prone to cracking and breaking, which increases maintenance costs, sometimes even approaching the cost of purchasing a new set. Furthermore, the use process does not consider the scenario where the electrode fluid needs to be recycled, resulting in extremely low recycling efficiency of the electrode fluid.

[0007] Therefore, it is necessary to develop a magnetic nucleic acid gel electrophoresis instrument to solve the above problems. Utility Model Content

[0008] The purpose of this invention is to design a magnetic nucleic acid gel electrophoresis instrument to solve the above problems.

[0009] This utility model achieves the above objectives through the following technical solutions: A magnetic suction type nucleic acid gel electrophoresis apparatus, comprising: Electrophoresis tank body; a raised platform is provided in the middle of the electrophoresis tank body, and an electrode wire installation and maintenance compartment is provided inside the first side wall of the electrophoresis tank body; electrode wire installation holes are provided at both ends of the bottom of the first side inner wall of the electrophoresis tank body. Two electrode wires; the electrode wires are installed through electrode wire mounting holes, the first end of the electrode wire is placed inside the electrophoresis tank, the middle part of the electrode wire is sealed and fixed inside the electrode wire mounting hole by sealing material, and the second end of the electrode wire is placed inside the electrode wire installation and maintenance chamber. Magnetic connector female; A magnetic connector female mounting hole is provided on the outer wall of the first side of the electrophoresis tank. The magnetic connector female is installed in the magnetic connector female mounting hole, and the second ends of the two electrode wires are electrically connected to the magnetic connector female respectively. The electrophoresis apparatus cover is an L-shaped plate structure, comprising a cover plate and side plates. The cover plate is used to cover the upper opening of the electrophoresis tank. The first end of the cover plate is connected to the upper end of the side plates. The side plates have mounting positions for magnetic connector male heads. Magnetic connector male; the magnetic connector male is installed in the mounting position of the magnetic connector male; the magnetic connector female and the magnetic connector male are magnetically connected.

[0010] Preferably, the sealing material is UV adhesive.

[0011] Furthermore, a pouring port is provided at the top of the second sidewall of the electrophoresis tank, and the second sidewall of the electrophoresis tank and the first sidewall are adjacent sidewalls.

[0012] Furthermore, one electrode wire near the pouring port is the negative electrode, and the other electrode wire is the positive electrode.

[0013] Furthermore, the lower end of the electrode wire installation and maintenance compartment is provided with an opening, which is closed by the bottom cover of the electrode wire installation and maintenance compartment.

[0014] Furthermore, handles for the electrophoresis apparatus cover are provided on both sides of the upper part of the cover plate.

[0015] Furthermore, multiple air vents for the electrophoresis apparatus are vertically pierced through the center of the cover plate.

[0016] Furthermore, the upper wall and side plate of the magnetic male connector mounting structure on the top cover of the electrophoresis apparatus are connected by a reinforcing rib structure.

[0017] The beneficial effects of this utility model are as follows: The magnetic connector female and magnetic connector male are connected by magnetic attraction. The magnetic connection basically eliminates the friction during the insertion and removal process of the magnetic connector female and magnetic connector male, avoiding wear and wobbling of the connector due to repeated insertion and removal, which can lead to poor contact and failure to conduct electricity, thus extending the service life of the equipment interface. In this application, the electrode wires are arranged to pass through the electrophoresis tank and then connected to the magnetic connector female. The magnetic connector female is also a magnetic connector female mounting hole installed on the side wall of the electrophoresis tank. Therefore, the power line connecting the electrode wire to the magnetic connector female does not need to be routed in the vertical direction and then in the horizontal direction. The power line is not easy to break, reducing safety hazards. The electrode wire in this application is fixed in position and does not require repeated insertion and removal, making it less prone to shaking and breakage. If the electrode wire connector in this application breaks, it is only necessary to open the bottom cover of the electrode wire installation and maintenance compartment, enter inside to replace the connector, and then reconnect it. There is no need to replace the whole thing, which saves costs.

[0018] In this application, the electrophoresis apparatus cover can be opened by pulling the handle of the electrophoresis apparatus cover with one hand, making it easy to operate; In this application, a pouring spout is provided on the electrophoresis tank to facilitate the pouring and recovery of electrode buffer solution, thus avoiding the situation where the electrode buffer solution is spilled and wasted during the pouring and recovery process. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the three-dimensional structure of the present invention. Figure 1 ; Figure 2 This is a schematic diagram of the three-dimensional structure of the present invention. Figure 2 ; Figure 3 This is an exploded view of the present invention; Figure 4 This is a bottom view of the present invention; Figure 5 This is a schematic diagram of the bottom structure of the electrophoresis apparatus cover in this utility model; Figure 6 This is a top view of the electrophoresis tank in this utility model; Figure 7 This is a schematic diagram of the electrode wire mounting hole in this utility model.

[0020] In the diagram: 1-Electrophoresis tank; 2-Electrophoresis apparatus cover; 3-Pour port; 4-Electrophoresis apparatus cover handle; 5-Magnetic connector female; 6-Magnetic connector male; 7-Electrophoresis apparatus cover vent; 8-Electrode wire installation and maintenance compartment bottom cover; 9-Electrophoresis apparatus cover magnetic connector male mounting structure; 10-Reinforcing rib structure; 11-Magnetic connector female mounting hole position; 12-Protruding platform inside the electrophoresis tank; 13-Magnetic connector male mounting position; 14-Electrode wire; 15-Electrode wire installation and maintenance compartment; 16-Electrode wire mounting hole. Detailed Implementation

[0021] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. The components of the embodiments of this utility model described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0022] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0023] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0024] In the description of this utility model, it should be understood that the terms "upper", "lower", "inner", "outer", "left", "right", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship that the utility model product is usually placed in during use, or the orientation or positional relationship that is commonly understood by those skilled in the art. They are only used to facilitate the description of this utility model and to simplify the description, and are not intended to indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0025] Furthermore, the terms "first," "second," etc., are used only to distinguish descriptions and should not be interpreted as indicating or implying relative importance.

[0026] In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, terms such as "set" and "connection" should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be a connection within two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0027] The specific embodiments of this utility model will now be described in detail with reference to the accompanying drawings.

[0028] like Figure 1-3 As shown in Figure 5-7, a magnetic suction nucleic acid gel electrophoresis apparatus includes: Electrophoresis tank 1; an inner protruding platform 12 is provided in the middle of the electrophoresis tank 1, and an electrode wire installation and maintenance compartment 15 is provided inside the first side wall of the electrophoresis tank 1; electrode wire installation holes 16 are provided at both ends of the bottom of the first side inner wall of the electrophoresis tank 1. Two electrode wires 14 are provided; the electrode wires 14 are installed through the electrode wire mounting holes 16, the first end of the electrode wires 14 is placed in the electrophoresis tank 1, the middle part of the electrode wires 14 is sealed and fixed in the electrode wire mounting holes 16 by UV glue, and the second end of the electrode wires 14 is placed in the electrode wire installation and maintenance chamber 15. Magnetic connector female head 5; A magnetic connector female head mounting hole 11 is provided on the outer wall of the first side of the electrophoresis tank 1. The magnetic connector female head 5 is installed in the magnetic connector female head mounting hole 11, and the second ends of the two electrode wires 14 are electrically connected to the magnetic connector female head 5 respectively. Electrophoresis apparatus cover 2; The electrophoresis apparatus cover 2 is formed into an L-shaped plate structure. The electrophoresis apparatus cover 2 includes a cover plate and a side plate. The cover plate is used to cover the upper opening of the electrophoresis tank 1. The first end of the cover plate is connected to the upper end of the side plate. The side plate has a mounting position 13 for a magnetic connector male. Magnetic connector male 6; magnetic connector male 6 is installed in the mounting position 13 of magnetic connector male; magnetic connector female 5 and magnetic connector male 6 are magnetically connected.

[0029] It should be noted that during installation, the magnetic connector male 6 should be inserted into the installation position 13 of the magnetic connector male from the side plate facing the electrophoresis tank 1.

[0030] In some embodiments, such as Figure 3 As shown, the top of the second side wall of the electrophoresis tank 1 is provided with a pouring port 3, and the second side wall of the electrophoresis tank 1 and the first side wall are adjacent side walls.

[0031] In some embodiments, one electrode wire 14 near the pouring port 3 is the negative electrode, and the other electrode wire 14 is the positive electrode.

[0032] In some embodiments, such as Figure 3 and 4 As shown, the lower end of the electrode wire installation and maintenance chamber 15 is provided with an opening, which is closed by the bottom cover 8 of the electrode wire installation and maintenance chamber.

[0033] In some embodiments, such as Figure 1-3 As shown, there are handles 4 on both sides of the top of the cover plate for the electrophoresis apparatus.

[0034] In some embodiments, such as Figure 3 As shown, there are two vertical ventilation holes 7 in the middle of the cover plate, and the two ventilation holes 7 are parallel to each other.

[0035] In some embodiments, such as Figure 3 As shown, the upper wall of the magnetic male connector mounting structure 9 on the top cover of the electrophoresis apparatus is connected to the side plate by a reinforcing rib structure 10.

[0036] Working principle: When performing electrophoresis, first place the pre-prepared agarose gel on the raised platform 12 inside the electrophoresis tank, with the gel spotting wells facing the negative electrode. Pour in the electrophoresis buffer solution just enough to cover the gel. Use a pipette to load the sample. After loading, align the male magnetic connector 6 inside the electrophoresis apparatus cover 2 with the female magnetic connector 5 inside the electrophoresis tank 1. Then, place the cover plate of the electrophoresis apparatus cover 2 on top of the electrophoresis tank 1. Pull the electrophoresis apparatus cover 2 towards the electrophoresis tank 1 until the female magnetic connector 5 and the male magnetic connector 6 are magnetically engaged. At this point, the electrophoresis apparatus cover 2 is in place. Connect the power supply, set the correct voltage, and electrophoresis can begin.

[0037] This application adopts innovative magnetic attraction technology, structural layout, and circuit layout, which solves the shortcomings of existing technologies in one go. This is also the first application of magnetic attraction technology in nucleic acid gel electrophoresis instrument, bringing a new direction to the development of nucleic acid electrophoresis instrument. This application has many advantages such as safety, durability, convenience, low maintenance cost, aesthetics, and good adaptability, and can completely replace existing nucleic acid electrophoresis instruments.

[0038] The above are merely preferred embodiments of this utility model. It should be noted that, for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of this utility model, and these improvements and modifications should also be considered within the scope of protection of this utility model.

Claims

1. A magnetic suction type nucleic acid gel electrophoresis apparatus, characterized in that, include: Electrophoresis tank body; a raised platform is provided in the middle of the electrophoresis tank body, and an electrode wire installation and maintenance compartment is provided inside the first side wall of the electrophoresis tank body; electrode wire installation holes are provided at both ends of the bottom of the first side inner wall of the electrophoresis tank body. Two electrode wires; the electrode wires are installed through electrode wire mounting holes, the first end of the electrode wire is placed inside the electrophoresis tank, the middle part of the electrode wire is sealed and fixed inside the electrode wire mounting hole by sealing material, and the second end of the electrode wire is placed inside the electrode wire installation and maintenance chamber. Magnetic connector female; A magnetic connector female mounting hole is provided on the outer wall of the first side of the electrophoresis tank. The magnetic connector female is installed in the magnetic connector female mounting hole, and the second ends of the two electrode wires are electrically connected to the magnetic connector female respectively. The electrophoresis apparatus cover is an L-shaped plate structure, comprising a cover plate and side plates. The cover plate is used to cover the upper opening of the electrophoresis tank. The first end of the cover plate is connected to the upper end of the side plates. The side plates have mounting positions for magnetic connector male heads. Magnetic connector male; the magnetic connector male is installed in the mounting position of the magnetic connector male; the magnetic connector female and the magnetic connector male are magnetically connected.

2. The magnetic suction type nucleic acid gel electrophoresis apparatus according to claim 1, characterized in that, The sealing material is UV adhesive.

3. The magnetic suction type nucleic acid gel electrophoresis apparatus according to claim 1, characterized in that, The top of the second sidewall of the electrophoresis tank is provided with a pouring port, and the second sidewall of the electrophoresis tank and the first sidewall are adjacent sidewalls.

4. The magnetic suction type nucleic acid gel electrophoresis apparatus according to claim 3, characterized in that, One electrode wire near the pouring spout is the negative electrode, and the other electrode wire is the positive electrode.

5. A magnetic suction nucleic acid gel electrophoresis apparatus according to claim 1, characterized in that, An opening is provided at the lower end of the electrode wire installation and maintenance compartment, which is sealed by the bottom cover of the electrode wire installation and maintenance compartment.

6. The magnetic suction type nucleic acid gel electrophoresis apparatus according to claim 1, characterized in that, Both sides of the cover plate are equipped with handles for the electrophoresis apparatus.

7. The magnetic suction type nucleic acid gel electrophoresis apparatus according to claim 1, characterized in that, Multiple air vents for the electrophoresis apparatus are vertically pierced through the middle of the cover plate.

8. A magnetic suction-type nucleic acid gel electrophoresis apparatus according to claim 1, characterized in that, The upper wall and side plate of the magnetic male connector mounting structure on the top cover of the electrophoresis apparatus are connected by a reinforcing rib structure.

Images