An antibody incubation cartridge with adjustable internal structure

Abstract

The utility model discloses an inside structure adjustable formula antibody incubation box, including antibody incubation box main part, installation post, the installation post is fixed in the inside of antibody incubation box main part. The utility model discloses be provided with the main partition post of connection in the middle, its outside via connecting seat, connecting groove and connecting block and with the sub -partition post of separate board card joint, and can according to single sample, multiple samples or different size PVDF film to its inside partition into different containing space's independent area, after the card joint, and the sealing washer of separate board bottom contacts with antibody incubation box main part, i.

Description

Technical Field

[0001] This utility model relates to the field of biomedical experimental equipment technology, and in particular to an antibody incubation box with adjustable internal structure. Background Technology

[0002] Western blotting (WB) is a common protein analysis technique used to identify specific proteins and perform qualitative and semi-quantitative analysis. The core concept of WB is the specific reaction between antigen and antibody. Proteins of different molecular weights are separated using denaturing gel SDS-PAGE, then transferred to a solid PVDF membrane. The membrane is blocked with skim milk and diluted with primary antibody dilution buffer. After the target protein and antibody are fully bound, excess unbound primary antibody is eluted with TBST elution buffer. Then, a corresponding secondary antibody labeled with HRP is added. This forms a protein + primary antibody + secondary antibody complex. When chemiluminescent solution is applied, fluorescence is produced at the sites of the target protein, allowing the target protein band to be visualized using a film or chemiluminescence analyzer. The key steps in Western blotting (WB) experiments include the detection of the target protein and the incubation of specific antibodies. Therefore, incubation chambers are required for incubating primary and secondary antibodies in WB experiments. However, the incubation chambers commonly used in existing WB experiments have a large internal space, which means that the antibody solution cannot be fully utilized when the sample volume is small, resulting in resource waste. In addition, the fixed structure of the incubation chamber is difficult to clean after the experiment, resulting in low reusability. At the same time, there is room for optimization. Therefore, it is necessary to design an antibody incubation chamber with an adjustable internal structure to solve the above-mentioned problems. Utility Model Content

[0003] The purpose of this invention is to provide an antibody incubation box with an adjustable internal structure to solve the problem of incubation boxes with inconvenient internal space adjustment mentioned in the background art.

[0004] To achieve the above objectives, this utility model provides the following technical solution: an antibody incubation box with adjustable internal structure, comprising an antibody incubation box body and mounting columns, wherein the mounting columns are all fixed inside the antibody incubation box body; The outer side of the mounting column is connected to a sub-separator column and a main separator column via mounting grooves. Connecting seats are uniformly fixed to the outer side of the main separator column, and each connecting seat has a connecting groove inside. Separator plates are fitted onto the outer side of each sub-separator column. Connecting blocks are fixed to one side of each separator plate and connected to the connecting grooves. A PVDF membrane is placed inside the antibody incubation box body. A peristaltic pump is located on one side of the antibody incubation box body, with an outlet pipe and an inlet pipe connected to both ends of the pump side. An inlet and an outlet are located on both sides of the antibody incubation box body. One side of the antibody incubation box body is connected to the outlet pipe via a first Luer lock interface, and the other side of the antibody incubation box body is connected to a reflux pipe via a second Luer lock interface. The other end of the reflux pipe is connected to an antibody storage tube. Microfluidic channels are provided at the bottom of each separator plate.

[0005] Preferably, the size of the mounting groove is matched with the size of the mounting column, and both the mounting groove and the mounting column are cylindrical.

[0006] Preferably, a second sealing gasket is fixed to the bottom end of the main partition column, and a first sealing gasket is fixed to the bottom end of the partition plate.

[0007] Preferably, both the second sealing gasket and the first sealing gasket are made of rubber, and the cross-section of the first sealing gasket is L-shaped.

[0008] Preferably, the main separator is connected to the middle position inside the antibody incubation box body, and the connectors are arranged in a cross shape on the outside of the main separator.

[0009] Preferably, the main body of the antibody incubation box is made of PTFE.

[0010] Preferably, the size of the connecting groove is matched with the size of the connecting block, and the height of the connecting block is less than the height of the partition plate.

[0011] Compared with the prior art, the beneficial effects of this utility model are: the adjustable internal structure antibody incubation box realizes the function of easy space adjustment; The system features a centrally located main separator column, whose outer side is connected to a sub-septum column with a separator plate via a connecting seat, connecting groove, and connecting block. This design allows for the internal division into independent regions with different containment spaces, suitable for single samples, multiple samples, or PVDF membranes of different sizes. After connection, the bottom sealing gasket of the separator plate contacts the main body of the antibody incubation box, ensuring a bottom-sealed state for each divided region. This prevents mixing of antibody solutions from different samples, ensuring experimental independence and accuracy. The flexible internal structure allows for adjustments to meet various experimental needs, optimize antibody utilization efficiency, and enhance the convenience of experimental operations. After internal space adjustment, the antibody solution only needs to cover the required experimental area, significantly reducing antibody usage and effectively saving antibody consumption. It is suitable for multiple stages of WB experiments, including incubation of primary and secondary antibodies and other experimental scenarios requiring specific antibody binding. At the same time, the quick-release snap-fit ​​design between the components facilitates cleaning and reuse, reducing experimental costs. Furthermore, compared with traditional incubation boxes, the incubation box with internal structure adjustment can achieve the goal of completely covering the incubation membrane while reducing diluent during shaker incubation, thus greatly saving experimental costs in a complete WB process. By employing a peristaltic pump, it is easy to clean and maintain. During use, the peristaltic pump draws antibody solution from the antibody reservoir and delivers it to the incubation chamber inlet. The solution then flows through a microfluidic channel to cover the PVDF membrane and is connected to the antibody reservoir via the outlet. This circulation of the antibody solution ensures that the antibody binds more fully to the target protein on the PVDF membrane, improving the sensitivity and accuracy of the experiment. The circulation also helps to distribute the antibody solution evenly on the PVDF membrane, reducing local concentrations that are too high or too low, thus making more efficient use of the antibody solution, reducing waste and improving experimental efficiency. Furthermore, the design with multiple Luer lock interfaces allows for adjustment of the interfaces during antibody incubation, providing flexibility. Attached Figure Description

[0012] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0013] Figure 1 This is a top view of the structure of this utility model; Figure 2 This is a schematic diagram of the first partitioned state structure of this utility model; Figure 3 This is a schematic diagram of the second partitioned state structure of this utility model; Figure 4This is a schematic diagram of the third partitioned state structure of this utility model; Figure 5 This is a schematic diagram of the fourth partitioned state structure of this utility model; Figure 6 This is a frontal cross-sectional view of the present invention. Figure 7 This is a three-dimensional structural diagram of the connector of this utility model; Figure 8 This is a three-dimensional structural diagram of the separator plate and sub-separator column of this utility model in their separated state.

[0014] The following are the labels in the diagram: 1. Antibody incubation box body; 2. PVDF membrane; 3. Separator plate; 4. Sub-septum; 5. Main septum; 6. Mounting column; 7. Connector seat; 8. Connecting groove; 9. First sealing gasket; 10. Mounting groove; 11. Connecting block; 12. Second sealing gasket; 13. Peristaltic pump; 14. Outlet tube; 15. First Luer lock interface; 16. Inlet tube; 17. Inlet; 18. Antibody storage tube; 19. Reflux tube; 20. Second Luer lock interface; 21. Outlet; 22. Microfluidic channel. Detailed Implementation

[0015] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0016] Please see Figures 1-8 One embodiment of this utility model is an antibody incubation box with an adjustable internal structure, comprising an antibody incubation box body 1 and a mounting column 6. The main body of the antibody incubation box is made of PTFE. Specifically, such as Figure 1 As shown, when in use, the PTFE material is used to adapt to the chemical reagent environment of WB experiments, and it has excellent durability and easy cleaning. The mounting columns 6 are all fixed inside the antibody incubation box body 1, and the outer side of the mounting columns 6 is connected to the sub-septum column 4 and the main septum column 5 respectively through the mounting groove 10; The size of the mounting groove 10 is matched with the size of the mounting post 6, and both the mounting groove 10 and the mounting post 6 are cylindrical. Specifically, such as Figure 6As shown, during use, the mounting groove 10 and mounting column 6 facilitate the installation and removal of the sub-separator column 4 and the main separator column 5. When the separator plate 3 is added, it can provide a seal. When the separator plate 3 is not added, the two PVDF membranes 2 can be separated, and the two PVDF membranes 2 will not overlap during shaking incubation. The bottom end of the main partition column 5 is fixed with a second sealing gasket 12, and the bottom end of the partition plate 3 is fixed with a first sealing gasket 9. Both the second sealing gasket 12 and the first sealing gasket 9 are made of rubber, and the cross section of the first sealing gasket 9 is L-shaped. Specifically, such as Figure 7 As shown, in use, the sealing effect of the separated space can be further enhanced by the first sealing gasket 9 and the second sealing gasket 12; The main separator 5 is connected to the middle position inside the main body 1 of the antibody incubation box, and the connectors 7 are arranged in a cross shape on the outside of the main separator 5. Specifically, such as Figure 1 As shown, during use, the connecting seats 7 are arranged in a cross shape on the outside of the main partition column 5, which can realize a variety of different forms of partitioning; Connecting seats 7 are evenly fixed on the outer side of the main partition column 5, and connecting grooves 8 are provided inside the connecting seats 7. The size of the connecting groove 8 is matched with the size of the connecting block 11, and the height of the connecting block 11 is less than the height of the partition plate 3. Specifically, such as Figure 6 As shown, in use, the assembly of the partition plate 3 can be facilitated by setting the connecting block 11; Each of the sub-separation columns 4 is fitted with a separator plate 3. Each of the separator plates 3 has a connecting block 11 fixed on one side, and the connecting block 11 is connected to the connecting groove 8. The body of the antibody incubation box 1 contains a PVDF membrane 2. A peristaltic pump 13 is provided on one side of the antibody incubation box 1, and the two ends of the peristaltic pump 13 are respectively connected to the outlet pipe 14 and the inlet pipe 16. The two sides of the antibody incubation box 1 are respectively provided with an inlet 17 and an outlet 21. One side of the antibody incubation box 1 is connected to the outlet pipe 14 through the first Luer lock interface 15. The other side of the antibody incubation box 1 is connected to the reflux pipe 19 through the second Luer lock interface 20. The other end of the reflux pipe 19 is connected to the antibody storage pipe 18. Each of the separator plates 3 has a microfluidic channel 22 at the bottom.

[0017] Working Principle: In use, this invention first divides the interior of a PVDF membrane 2 into independent regions with different containment spaces, based on whether it contains a single sample, multiple samples, or different sizes. The sub-segment column 4 is then snapped into place with the main segment column 5 via connecting blocks 11 and connecting grooves 8. The separator plate 3 is fitted onto both sides of the sub-segment column 4, with its outer side conforming to the inner wall of the box. This ensures that after snapping, the bottom sealing gasket of the separator plate 3 contacts the antibody incubation box body 1, resulting in a bottom-sealed state for the divided region. This prevents the mixing of antibody solutions between different samples, ensuring the independence and accuracy of the experiment. Furthermore, compared to traditional incubation boxes, this incubation box with its adjustable internal structure allows for incubation on a shaker. This method achieves the goal of completely covering the incubation membrane while reducing the amount of diluent, thus significantly saving experimental costs in a complete Western blot (WB) procedure. During operation, the peristaltic pump 13 draws the antibody solution from the antibody reservoir 18 and delivers it to the incubation box inlet 17. The solution then covers the area on the PVDF membrane 2 via the microfluidic channel 22 and is connected to the antibody reservoir 18 through the outlet 21. By circulating the antibody solution, it is ensured that the antibody binds more fully to the target protein on the PVDF membrane 2, improving the sensitivity and accuracy of the experiment. The circulating flow also helps to evenly distribute the antibody solution on the PVDF membrane 2, reducing the possibility of local concentrations being too high or too low.

[0018] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0019] The device embodiments described above are merely illustrative. The units described as separate components may or may not be physically separate. The components shown as units may or may not be physical units; that is, they may be located in one place or distributed across multiple network units. Some or all of the modules can be selected to achieve the purpose of this embodiment according to actual needs. Those skilled in the art can understand and implement this without any creative effort.

[0020] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model, and not to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model.

Claims

1. An antibody incubation box with adjustable internal structure, comprising an antibody incubation box body (1) and mounting posts (6), wherein the mounting posts (6) are all fixed inside the antibody incubation box body (1); Its features are: The outer side of the mounting column (6) is connected to the sub-separator column (4) and the main separator column (5) respectively through the mounting groove (10). The outer side of the main separator column (5) is uniformly fixed with the connecting seat (7), and the interior of the connecting seat (7) is provided with the connecting groove (8). The outer side of the sub-separator column (4) is fitted with the separator plate (3). The side of the separator plate (3) is fixed with the connecting block (11), and the connecting block (11) is connected to the connecting groove (8). The body of the antibody incubation box (1) contains a PVDF membrane (2), and the side of the body of the antibody incubation box (1) is provided with a peristaltic pump (13). The peristaltic pump (13) has an outlet pipe (14) and an inlet pipe (16) connected to its two ends. The antibody incubation box body (1) has an inlet (17) and an outlet (21) on its two sides. The antibody incubation box body (1) is connected to the outlet pipe (14) through the first Luer lock interface (15) on one side. The antibody incubation box body (1) is connected to the reflux pipe (19) through the second Luer lock interface (20) on the other side. The other end of the reflux pipe (19) is connected to the antibody storage tube (18). The bottom end of the partition plate (3) is provided with a microfluidic channel (22).

2. The adjustable antibody incubation box according to claim 1, characterized in that: The size of the mounting groove (10) is matched with the size of the mounting column (6), and both the mounting groove (10) and the mounting column (6) are cylindrical.

3. The adjustable internal structure antibody incubation box according to claim 1, characterized in that: The bottom end of the main partition column (5) is fixed with a second sealing gasket (12), and the bottom end of the partition plate (3) is fixed with a first sealing gasket (9).

4. The internally adjustable antibody incubation box according to claim 3, characterized in that: Both the second sealing gasket (12) and the first sealing gasket (9) are made of rubber, and the cross-section of the first sealing gasket (9) is L-shaped.

5. The internally adjustable antibody incubation box according to claim 1, characterized in that: The main separator (5) is connected to the middle position inside the antibody incubation box body (1), and the connectors (7) are arranged in a cross shape on the outside of the main separator (5).

6. The internally adjustable antibody incubation box according to claim 1, characterized in that: The main body (1) of the antibody incubation box is made of PTFE.

7. The internally adjustable antibody incubation box according to claim 1, characterized in that: The size of the connecting groove (8) is matched with the size of the connecting block (11), and the height of the connecting block (11) is less than the height of the partition plate (3).

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