A combined device for embryo freezing operations

Abstract

The utility model provides a kind of combined device for embryo freezing operation, including bottom plate, cover plate and freezing carrier pole;The bottom plate one end is equipped with placing dish, and the other end is equipped with card tray;The placing dish is equipped with several rows of drip groove;The card tray is equipped with several placing grooves for placing freezing carrier pole, and placing groove and drip groove are in the same horizontal line;The card tray top side is equipped with'convex'type limiting slot, and'convex'type limiting slot bottom is hollowly provided;The cover plate is equipped with fixed groove matched with carrier pole placing groove;Cover plate side is equipped with'T'type limiting block matched with'convex'type limiting slot.The utility model structure is simple, avoids the instability when operator manually takes freezing carrier pole, reduces the number of microscope field of view adjustment, and can realize multi-tube embryo simultaneous transfer, to improve transfer efficiency and security.

Description

Technical Field

[0001] This invention belongs to the field of embryo cryopreservation technology, and specifically relates to a combined device for embryo cryopreservation operations. Background Technology

[0002] In modern reproductive medicine, assisted reproductive technology has brought hope to countless infertile couples. Embryo freezing and thawing is a core technology in assisted reproduction, helping to improve pregnancy success rates and increase the chances of having children. In recent years, due to factors such as declining fertility among young women, delayed childbearing age, and the increased risk of ovarian function damage after surgery or chemotherapy, the demand for fertility preservation has increased, leading to an exponential increase in the number of embryo freezing cycles worldwide. Embryo freezing refers to the process of using special cryoprotectants and cooling procedures to stop the metabolism of embryos at -196°C, rapidly putting them into a "hibernation" state.

[0003] Before vitrification of embryos, a cryostat dish must first be prepared. Embryos are then transferred into the cryostat dish for osmotic equilibration and dehydration. Finally, a pasteurized pipette is used to place the embryo on top of a cryostat rod, which is then quickly immersed in liquid nitrogen. However, currently there is no integrated device for holding the cryostat dish and cryostat rod. The cryostat dish and cryostat rod are placed separately, making it difficult to maintain a stable horizontal position. Transferring embryos from the cryostat dish to the cryostat rod requires adjusting the microscope's field of view, prolonging the loading time and hindering rapid freezing. Furthermore, the current technique only allows for freezing one embryo at a time, preventing simultaneous freezing of multiple embryos and resulting in low efficiency. The lack of a dedicated device for securing multiple cryostat rods forces some laboratory personnel to temporarily fix multiple cryostat rods to the culture dish lid with tape to freeze multiple embryos simultaneously. These practices all negatively impact the efficiency and stability of the vitrification process. Utility Model Content

[0004] To address the aforementioned problems with current embryo vitrification freezing methods, this invention provides a combined device for embryo freezing operations. This device fixes a freezing rod to one side of the operating dish for embryo transfer, enabling the simultaneous transfer of multiple embryos while maintaining the freezing rod in the same position. This improves the simplicity and safety of embryo freezing operations and has broad application prospects.

[0005] This utility model is achieved through the following technical solution: A combined device for embryo freezing operations includes a base plate, a cover plate, and a freezing rod; One end of the described bottom plate is provided with a placement dish, and the other end is provided with a holder; a plurality of rows of drip tanks are arranged inside the placement dish; the holder is provided with a plurality of placement slots for placing frozen loading rods, and the placement slots and the drip tanks are at the same horizontal level; two sides of the top of the holder are provided with "convex"-shaped limiting grooves, and the bottom of each "convex"-shaped limiting groove is arranged in a hollowed-out manner; The described cover plate is provided with fixing slots matching the loading rod placement slots; two sides of the cover plate are provided with "T"-shaped limiting blocks matching the "convex"-shaped limiting grooves.

[0006] The working principle of the present utility model is as follows: Before performing embryo transfer, firstly, the equilibration solution and the vitrification solution are respectively dropped into each row of the drip tanks, then a plurality of frozen loading rods are placed in the placement slots, with the slide ends of the frozen loading rods close to the placement dish, then the cover plate is covered on the holder, and the cover plate is fixed by inserting the "T"-shaped limiting blocks into the "convex"-shaped limiting grooves and pushing inward for clamping. Then embryo transfer is started, the entire device is placed under a microscope, and the field of view of the microscope is adjusted so that both the drip tanks and the slide ends of the frozen loading rods can be seen at the same time. Next, a Pasteur pipette is used to transfer the embryos into the equilibration solution for standing for 7-10 minutes, after standing, the Pasteur pipette is used to transfer the embryos into the vitrification solution, and within 60 seconds, the Pasteur pipette is used to transfer the embryos in the vitrification solution together with a small amount of vitrification solution to the slide ends of the frozen loading rods. After all embryos are fully transferred to the slide ends of all the frozen loading rods according to the loading requirements, the entire combined device is moved into a liquid nitrogen storage tank, with the holder end facing downward, the cover plate is pushed to make the "T"-shaped limiting blocks reach the bottom of the "convex"-shaped limiting grooves, then the cover plate can be taken out upward, and the frozen loading rods fall into the liquid nitrogen storage tank for long-term preservation.

[0007] As a further improvement of the present utility model, the described placement slots are arranged in communication with the placement dish.

[0008] The communication between the placement slots and the placement dish facilitates moving the Pasteur pipette at a relatively low horizontal level to transfer the embryos from the equilibration solution to the slide ends of the frozen loading rods, improving the smoothness of transfer.

[0009] As a further improvement of the present utility model, one side of the described bottom plate is provided with a connecting slot, and the symmetrical position on the other side is provided with a connecting block.

[0010] The bottom plate is clamped with the connecting slot through the connecting block, so that more frozen loading rods can be loaded with embryos at the same time, realizing the simultaneous operation of a large number of embryo transfers and improving the transfer efficiency. The clamped bottom plate is also reinforced to prevent offset or shake during the embryo transfer process from affecting the operation.

[0011] As a further improvement of the present utility model, two sides of the described placement slot are provided with alignment lines.

[0012] The provision of alignment lines can more conveniently unify the positions of multiple cryo-load rods, making the slide-carrying ends of the cryo-load rods as close to the placement dish as possible, which facilitates embryo transfer.

[0013] Compared with the prior art, the beneficial effects of the present utility model are as follows: 1. The device of the present utility model fixes the cryo-load rods at one end of the placement dish, which avoids the instability when the operator manually takes the cryo-load rods, and also reduces the number of times of adjusting the microscope field of view, thereby improving the transfer efficiency and safety.

[0014] 2. The overall structure of the present utility model is simple, which can realize the simultaneous transfer of multiple tubes of embryos. After transfer, the cryo-load rods can be separated from the holder by gently pushing the cover plate, and both loading and separation are easy to operate, which further improves the transfer efficiency. Brief Description of the Drawings

[0015] Figure 1 is a schematic diagram of the overall structure of the device of the present utility model.

[0016] Figure 2 is a schematic structural diagram of the base plate.

[0017] Figure 3 is a schematic structural diagram of the cover plate.

[0018] Figure 4 is a schematic structural diagram of the "convex"-shaped limiting groove, Figure 5 is a schematic diagram of the device connection structure of one embodiment.

[0019] Reference signs of the drawings: 1 - base plate, 2 - cover plate, 3 - placement dish, 4 - drip tank, 5 - holder, 6 - placement groove, 7 - "convex"-shaped limiting groove, 8 - fixing groove, 9 - "T"-shaped limiting block, 10 - cryo-load rod, 11 - alignment line, 12 - connecting groove, 13 - connecting block. Detailed Description of the Embodiments

[0020] The present utility model will be further described below with reference to the accompanying drawings. Embodiment 1

[0021] As shown in Figure 1 a combined device for embryo cryopreservation operation comprises a base plate 1, a cover plate 2 and cryo-load rods 10; The base plate 1 is as shown in Figure 2 one end is provided with a placement dish 3, and the other end is provided with a holder 5; a plurality of rows of drip tanks 4 are arranged in the placement dish 3; the holder 5 is provided with a plurality of placement grooves 6 for placing the cryo-load rods 10, and the placement grooves 6 and the drip tanks 4 are at the same horizontal line; two sides of the top of the holder 5 are provided with "convex"-shaped limiting grooves 7, the bottoms of the "convex"-shaped limiting grooves 7 are arranged in a hollow-out manner, as shown in Figure 4 As shown.

[0022] like Figure 3 As shown, the cover plate 2 is provided with a fixing groove 8 that matches the rod placement groove 6; the cover plate 2 is provided with "T" shaped limiting blocks 9 on both sides that match the "convex" shaped limiting groove 7.

[0023] The working principle of this embodiment is as follows: Before embryo transfer, equilibration solution and vitrification solution are first dripped into each dropper 4. Then, multiple cryopreservation rods 10 are placed in the placement trough 6, with the slide end of the cryopreservation rod 10 close to the placement dish 3. Then, the cover plate 2 is placed on the card holder 5. The cover plate 2 is fixed by inserting the "T"-shaped limiting block 9 into the "convex"-shaped limiting groove 7 and pushing it inward. Embryo transfer begins. The entire apparatus is placed under a microscope, and the microscope field of view is adjusted so that the slide end of the dropper trough 4 and the cryopreservation rod 10 can be seen simultaneously. At room temperature, the embryos are transferred from the culture medium to the equilibration solution for 7-10 minutes. Then, the embryos are transferred to the vitrification solution. Within 60 seconds, the embryos in the vitrification solution are transferred to the slide end of the cryopreservation rod 10 according to the loading requirements. The entire combined apparatus is quickly moved into the liquid nitrogen storage tank with one end of the tray 5 facing down. The cover plate 2 is pushed so that the "T"-shaped limiting block 9 reaches the bottom of the "convex"-shaped limiting groove 7. Then, the cover plate 2 can be removed upwards, and the cryopreservation rod 10 falls into the liquid nitrogen storage tank for long-term preservation. Example 2

[0024] This embodiment is a further improvement based on Embodiment 1, as detailed below: The placement slot 6 is connected to the placement dish 3.

[0025] The working principle of this embodiment is the same as that of embodiment 1. The placement groove 6 is connected to the placement dish 3, which facilitates the movement of the Pasteur tube at a lower level to transfer the embryo from the vitrified liquid to the slide end of the cryopreservation rod 10, thereby improving the stability of the transfer. Example 3

[0026] This embodiment is a further improvement based on embodiment 2, as detailed below: The base plate 1 has a connecting groove 12 on one side and a connecting block 13 at a symmetrical position on the other side.

[0027] The working principle of this embodiment is the same as that of embodiment 2. The base plate 1 is engaged with the connecting groove 12 via the connecting block 13, enabling a larger number of cryogenic carrier rods 6 to load embryos simultaneously. Figure 5 As shown, this allows for the simultaneous transfer of a large number of embryos, improving transfer efficiency. The base plate 1 is also reinforced to prevent displacement or shaking during embryo transfer from affecting the operation. Example 4

[0028] This embodiment is a further improvement based on embodiment 3, as detailed below: Alignment lines 11 are provided on both sides of the placement slot 6.

[0029] The working principle of this embodiment is the same as that of embodiment 3. Setting the alignment line 11 makes it easier to unify the position of multiple cryopreservation rods 10, so that the slide end of the cryopreservation rod 10 is as close as possible to the placement dish 3, which facilitates embryo transfer.

[0030] The above embodiments are merely exemplary embodiments of this utility model and are not intended to limit this utility model. The scope of protection of this utility model is defined by the claims. Those skilled in the art can make various modifications or equivalent substitutions to this utility model within its substance and scope of protection, and such modifications or equivalent substitutions should also be considered to fall within the scope of protection of this utility model.

Claims

1. A combined apparatus for embryo cryopreservation, characterized in that: Includes a base plate (1), a cover plate (2), and a refrigeration support rod (10). The base plate (1) has a placement dish (3) at one end and a card holder (5) at the other end; the placement dish (3) has several rows of drip grooves (4); the card holder (5) has several placement slots (6) for placing the cryogenic carrier rod (10), and the placement slots (6) and the drip grooves (4) are on the same horizontal line; the card holder (5) has "convex" shaped limiting slots (7) on both sides of the top, and the bottom of the "convex" shaped limiting slots (7) is hollowed out; The cover plate (2) is provided with a fixing groove (8) that matches the rod placement groove (6); the cover plate (2) is provided with "T" shaped limiting blocks (9) on both sides that match the "convex" shaped limiting groove (7).

2. The combined apparatus for embryo cryopreservation according to claim 1, characterized in that: The placement slot (6) is connected to the placement dish (3).

3. The combined apparatus for embryo cryopreservation according to claim 1, characterized in that: The base plate (1) has a connecting groove (12) on one side and a connecting block (13) on the other side at a symmetrical position.

4. The combined apparatus for embryo cryopreservation according to claim 1, characterized in that: Alignment lines (11) are provided on both sides of the placement slot (6).

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