A preservation box for cell recovery

Abstract

The utility model relates to cell preservation technical field especially a kind of cell recovery is with storage box, including, storage component, including box bottom, fixedly connected in the test tube card strip of box bottom inner wall and rotationally connected in the box cover of box bottom outer wall;And, adjusting component, including the fixed disc of fixedly connected in the lower end surface of test tube card strip, rotationally connected in the rotating disc of fixed disc lower end surface and the occlusion disc of annular distribution in the inner wall of rotating disc, storage component still includes the through slot of being opened in the outer wall of test tube card strip, the outer wall of box bottom and box cover is opened with the hollow slot corresponding with through slot, the utility model can assist quick thawing cell by storage component, prevent the water in frozen storage test tube from being immersed to contaminate cell, maximize guarantee cell activity, make scientific research smoothly carry out, compare peregrine can play protective effect when the burst pipe phenomenon occurs, simultaneously by adjusting component can be adjusted clamping according to different size test tube, improve the practicality and application range of device.

Description

Technical Field

[0001] This utility model relates to the field of cell preservation technology, and in particular to a cell resuscitation preservation box. Background Technology

[0002] In the field of biotechnology, there is a need to retrieve cells that have been stored in liquid nitrogen for a long time for scientific research applications. This requires rapid thawing in a 37-degree water bath to maximize cell viability. Controlling the thawing time and temperature is a key condition for ensuring cell viability. At the same time, when the cryovials containing the cells are taken out of the liquid nitrogen environment, the storage temperature is instantly transferred from -196 degrees to 37 degrees. Due to the instantaneous change in the internal and external pressure difference, the cryovials often burst, posing a certain danger to the operators.

[0003] In traditional water bath resuscitation, cryovials are typically immersed in 37-degree water and agitated. However, due to varying cell preservation needs and practical requirements, cryovial sizes differ, necessitating the replacement of components in the preservation box with different dimensions each time cells are revived. This reduces efficiency. Furthermore, the significant temperature difference between the inside and outside of the cryovials during immersion can cause deformation and gaps at the cryovial cap threads, allowing water to enter and contaminate the cells. Contaminated cells must be discarded, resulting in loss. Utility Model Content

[0004] In view of the problems existing in the above or prior art, this utility model is proposed.

[0005] Therefore, the purpose of this invention is to provide a cell resuscitation preservation box.

[0006] To solve the above-mentioned technical problems, this utility model provides the following technical solution: a cell resuscitation preservation box, comprising: a preservation component, including a box bottom, a test tube clip fixedly connected to the inner wall of the box bottom, and a box cover rotatably connected to the outer wall of the box bottom; and an adjustment component, including a fixed plate fixedly connected to the lower end face of the test tube clip, a rotating plate rotatably connected to the lower end face of the fixed plate, and a sealing plate distributed in a ring on the inner wall of the rotating plate.

[0007] As a preferred embodiment of the cell resuscitation preservation box of this utility model, the preservation component further includes a through groove formed on the outer wall of the test tube clip, and the outer walls of the box bottom and the box cover are both provided with hollow grooves corresponding to the through groove.

[0008] As a preferred embodiment of the cell resuscitation preservation box of this utility model, the adjustment component further includes a limiting block and a guide rod respectively fixedly connected to the upper end face and the lower end face of the sealing plate, and the inner wall of the fixing plate is provided with a limiting groove for the guide rod to slide.

[0009] As a preferred embodiment of the cell resuscitation preservation box of this utility model, the outer wall of the rotating disk is provided with a movable groove for the guide rod to slide, the end face of the fixed disk is provided with a sliding groove, a sliding block is slidably connected inside the sliding groove, and a connecting rod is fixedly connected to the end face of the sliding block.

[0010] In a preferred embodiment of the cell resuscitation preservation box of this utility model, the end face of the rotating disk is integrally formed with a first mounting block, the end face of the connecting rod is fixedly connected with a first fixing block, and the outer wall of the first fixing block is fixedly connected with an embedding block.

[0011] In a preferred embodiment of the cell resuscitation preservation box of this utility model, a second fixing block is fixedly connected to the outer wall of the first mounting block, a card plate is fixedly connected to the inner wall of the second fixing block, and the embedding block can be embedded into the inner side of the card plate.

[0012] In a preferred embodiment of the cell resuscitation preservation box of this utility model, the end face of the fixing plate is integrally formed with a second mounting block, the outer wall of the first mounting block is fixedly connected with a spring, and the end of the spring is fixedly connected to the end face of the second mounting block.

[0013] As a preferred embodiment of the cell resuscitation preservation box of this utility model, the upper end face of the test tube clip is provided with a slot, and a water-blocking cover is installed on the inner wall of the test tube clip, the end face of the water-blocking cover being adapted to the slot.

[0014] The beneficial effects of this cell resuscitation preservation box are as follows: This invention can assist in the rapid thawing of cells through the preservation components, prevent water from entering the cryopreservation tubes and thus contaminating the cells, maximize cell viability, and ensure the smooth progress of scientific research. Compared with penguins, it can also provide protection in the event of tube bursting. At the same time, the adjustable components can be adjusted to hold different sized test tubes, improving the practicality and applicability of the device. Attached Figure Description

[0015] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of the overall structure of a cell resuscitation preservation box.

[0017] Figure 2 This is a schematic diagram of the preservation components of a cell resuscitation preservation box.

[0018] Figure 3 This is a schematic diagram of the regulating component structure of a cell resuscitation preservation box.

[0019] Figure 4 A schematic diagram and a magnified view of the disassembled structure of the adjustment components of a cell resuscitation preservation box.

[0020] Figure 5 This is a schematic diagram of the fixation tray structure of a cell resuscitation preservation box.

[0021] Figure 6 This is a schematic diagram of the sealing disc structure of a cell resuscitation preservation box.

[0022] Figure 7 This is a schematic diagram of the test tube clip structure of a cell resuscitation preservation box.

[0023] Figure 8 This is a schematic diagram of the water-blocking structure of a cell resuscitation preservation box.

[0024] The components include: 1. Storage component; 11. Box bottom; 12. Test tube retainer strip; 13. Through groove; 14. Box lid; 15. Hollowed-out groove; 2. Adjustment component; 21. Fixing plate; 22. Limiting groove; 23. Rotating plate; 24. Sealing plate; 25. Guide rod; 26. Limiting block; 27. Moving groove; 28. Card slot; 29. ​​Sliding groove; 210. Sliding block; 211. Connecting rod; 212. First mounting block; 213. First fixing block; 214. Card plate; 215. Embedding block; 216. Second fixing block; 217. Spring; 218. Second mounting block; 219. Water shield. Detailed Implementation

[0025] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings.

[0026] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0027] Secondly, the term "an embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that excludes other embodiments.

[0028] Reference Figure 1 , Figure 2 and Figure 3 A cell resuscitation preservation box includes a preservation component 1, comprising a box bottom 11, a test tube clip 12 fixedly connected to the inner wall of the box bottom 11, and a box lid 14 rotatably connected to the outer wall of the box bottom 11. Both the box bottom 11 and the box lid 14 are made of metal. The box bottom 11 is folded up on three sides and open on one side, and the box lid 14 is also folded up on three sides and open on one side, corresponding to the dimensions of the folded-up sides of the box bottom 11. The width and height of the box lid 14 are slightly larger than those of the box bottom 11. The folded-up sides of the box lid 14 have arc-shaped grooves at the points where the box lid 14 is riveted to the test tube clip 12, exposing the rivets to prevent jamming and friction, while also facilitating finger gripping of the box bottom 11.

[0029] The test tube clip 12 is folded up at both ends and riveted to the folded-up parts on both sides of the bottom of the box 11. The width of the test tube clip 12 is the same as the width of the folded-up parts at the bottom of the box 11. At the same time, the test tube clip 12 is riveted to the upper part of the hollowed-out bottom of the box 11. After the cryopreservation tube is placed in, the clip is located at the lower part of the cryopreservation tube cap.

[0030] The adjustment component 2 includes a fixed plate 21 fixedly connected to the lower end face of the test tube clip 12, a rotating plate 23 rotatably connected to the lower end face of the fixed plate 21, and sealing plates 24 arranged in a ring on the inner wall of the rotating plate 23. The fixed plates 21 are fixed in a fixed state, with the same number as the test tubes and through-slots 13, and are fixedly installed on the lower end face of the test tube clip 12. Each fixed plate 21 is coaxially installed with the through-slot 13. The rotating plate 23 is rotatably connected to the lower end face of the fixed plate 21. There are six sets of sealing plates 24 within a single rotating plate 23, and the overall design is triangular. A through-slot 13 needs to be opened at the center of the outer wall of the fixed plate 21, and its size is adapted to the maximum opening range of the sealing plate 24.

[0031] Specifically, the storage component 1 also includes a through groove 13 formed on the outer wall of the test tube holder 12, and hollow grooves 15 corresponding to the through groove 13 are formed on the outer walls of the box bottom 11 and the box lid 14. The test tube holder 12 has several through grooves 13 in the middle. The through grooves 13 need to have a certain size to ensure that the adjustment component 2 does not interfere with the through grooves 13 during adjustment. This invention uses a circle as an example, but is not limited to a circle. The number of through grooves 13 is determined as needed.

[0032] The bottom 11 and the folded-down portion are both hollowed out. The shape and size of the hollowed-out groove 15 are determined according to the diameter of the cryopreservation tube, ensuring that the cryopreservation tube cannot slide out of the bottom 11 and allowing water to flow through the box.

[0033] Furthermore, the adjustment assembly 2 also includes a limiting block 26 fixedly connected to the upper end face of the sealing disk 24 and a guide rod 25 fixedly connected to the lower end face. The inner wall of the fixed disk 21 is provided with a limiting groove 22 for the guide rod 25 to slide. The limiting block 26 is a square block structure, and the guide rod 25 needs to extend to the outside of the rotating disk 23. The limiting block 26 and the guide rod 25 limit the movement direction of the rotating disk 23.

[0034] Reference Figures 3-8 The outer wall of the rotating disk 23 has a sliding groove 27 for the guide rod 25 to slide. The end face of the fixed disk 21 has a sliding groove 29. A sliding block 210 is slidably connected inside the sliding groove 29. A connecting rod 211 is fixedly connected to the end face of the sliding block 210. The number of sliding grooves 27 is the same as that of the guide rods 25. The sliding groove 27 is a short straight groove design to limit the movement direction of the guide rod 25. The sliding groove 29 is opened on part of the end face of the fixed disk 21 to ensure that the sliding block 210 has a certain sliding stroke. It is fixedly connected to the first fixed block 213 through the connecting rod 211.

[0035] Specifically, the end face of the rotating disk 23 is integrally formed with a first mounting block 212, the end face of the connecting rod 211 is fixedly connected to a first fixing block 213, and the outer wall of the first fixing block 213 is fixedly connected to an embedded block 215. The first mounting block 212 is connected to the rotating disk 23, allowing the second fixing block 216 to pass through the mounting platform, and facilitating personnel control of the rotation of the rotating disk 23. The embedded block 215 is fixedly connected to the first fixing block 213.

[0036] Furthermore, a second fixing block 216 is fixedly connected to the outer wall of the first mounting block 212, and a retaining plate 214 is fixedly connected to the inner wall of the second fixing block 216. An inserting block 215 can be inserted into the inner side of the retaining plate 214. The inner wall of the second fixing block 216 is provided with two sets of movable retaining plates 214. The connection between the first fixing block 213 and the second fixing block 216 is achieved by inserting the inserting block 215 into the retaining plate 214.

[0037] Reference Figures 3-8The end face of the fixed disk 21 is integrally formed with a second mounting block 218. A spring 217 is fixedly connected to the outer wall of the first mounting block 212, and the end of the spring 217 is fixedly connected to the end face of the second mounting block 218. The bottom of the second mounting block 218 is at the same height as the lower end face of the rotating disk 23. The second mounting block 218 is connected to the first mounting block 212 through the spring 217. The elastic force of the spring 217 is a tension force, so that the rotating disk 23 always maintains a clockwise rotation state. At the same time, self-locking is achieved through the connection between the embedded block 215 and the locking plate 214.

[0038] Specifically, the upper end face of the test tube clip 12 has a slot 28, and a water baffle 219 is installed on the inner wall of the test tube clip 12. The end face of the water baffle 219 is adapted to the slot 28. The water baffle 219 is also provided on the outer wall of the adjustment component 2. The upper and lower ends of the water baffle 219 have a larger diameter, while the middle part is narrower and has the same diameter as the cryopreservation tube used in this revival. The upper end face of the water baffle 219 is adapted to the slot 28 on the upper end face of the test tube clip 12, thus achieving the installation of the water baffle 219 through the snap-fit ​​with the slot 28. Simultaneously, the bottom of the water baffle 219 can block the oscillating water flow inside the preservation box, reducing the amount of water passing through the gap between the through groove 13 and the test tube.

[0039] Working principle: Open the lid 14, insert the appropriate number of cryopreservation tubes into the slots 13 of the test tube clips 12, close the lid 14. At the top of the preservation box, after the test tube clips 12 are inserted into the test tubes, they form a sealed space with the bottom 11 and the lid 14, preventing water from entering the test tube caps. Even if a small amount of water enters, it cannot enter the tube, preventing contamination of the cells inside the tube. At the bottom of the preservation box, the bottom 11 and the lid 14 have corresponding perforated slots 15, exposing the cryopreservation tubes inside the box. Pick up the preservation box and put it into 37-degree water. The depth of the preservation box in the water is the perforated part of the preservation box, and the non-perforated part of the preservation box is above the water surface. The preservation box is rapidly and repeatedly shaken in the water, and the water flows through the perforated part, quickly reviving the cells in 37-degree water. During the revival process, if a tube bursts, the metal preservation box can effectively prevent the tube from flying out and causing injury.

[0040] The water shield 219 is rotated according to the size of the test tube used in this cell resuscitation. The water shield 219 is locked onto the end face of the test tube clip 12 through the slot 28, controlling the first mounting block 212 to rotate. The first mounting block 212 drives the rotating disk 23 to rotate along the bottom of the fixed disk 21. Since the guide rod 25 on the lower end face of the sealing disk 24 extends to the outside of the moving groove 27 on the end face of the rotating disk 23, the rotation of the rotating disk 23 will drive the guide rod 25, so that the six sets of sealing disks 24 start to move at the same time. At the same time, the limiting block 26 on the upper end face of the sealing disk 24 corresponds to the limiting groove 22 opened on the inner wall of the fixed disk 21. Thus, the connection between the limiting groove 22 and the limiting block 26 makes the sealing disk 24 slide only along the limiting groove 22, thereby enabling the six sets of sealing disks 24 to move synchronously and gradually open the channel between them. When the channel reaches the diameter of the test tube, the movement of the first mounting block 212 stops.

[0041] At this time, the control slider 210 slides along the slide groove 29, thereby driving the first fixed block 213 to move synchronously through the connecting rod 211. The embedding block 215 on the end face of the first fixed block 213 is inserted into the two sets of clamping plates 214 on the end face of the second fixed block 216. The connection between the clamping plate 214 and the embedding block 215 realizes the connection between the first fixed block 213 and the second fixed block 216, thereby keeping the rotating disk 23 in a fixed state and fixing the channel size formed between the sealing disks 24. Thus, the adjustment of the adjustment component 2 can meet the placement of test tubes of different sizes and specifications. With the help of the water shield 219, the gap between the through groove 13 and the rotating disk 23 and the cryopreservation tube can be blocked, thereby protecting the cryopreservation tube and preventing water from entering the cryopreservation tube through the gap and causing the cells in the cryopreservation tube to come into contact with water and be contaminated.

[0042] It should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and are not intended to limit it. Although this utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solution of this utility model without departing from the spirit and scope of the technical solution of this utility model, and all such modifications or substitutions should be covered within the scope of the claims of this utility model.

Claims

1. A cell resuscitation preservation box, characterized in that: include, The storage component (1) includes a box bottom (11), a test tube holder (12) fixedly connected to the inner wall of the box bottom (11), and a box lid (14) rotatably connected to the outer wall of the box bottom (11); and, The adjustment assembly (2) includes a fixed disk (21) fixedly connected to the lower end face of the test tube clip (12), a rotating disk (23) rotatably connected to the lower end face of the fixed disk (21), and a sealing disk (24) distributed in a ring on the inner wall of the rotating disk (23).

2. The cell resuscitation preservation box as described in claim 1, characterized in that: The storage component (1) also includes a through groove (13) formed on the outer wall of the test tube strip (12), and the outer walls of the box bottom (11) and the box cover (14) are provided with hollow grooves (15) corresponding to the through groove (13).

3. A cell resuscitation preservation box as described in claim 2, characterized in that: The adjustment assembly (2) also includes a limiting block (26) fixedly connected to the upper end face of the sealing disc (24) and a guide rod (25) fixedly connected to the lower end face. The inner wall of the fixed disc (21) is provided with a limiting groove (22) for the guide rod (25) to slide.

4. A cell resuscitation preservation box as described in claim 3, characterized in that: The outer wall of the rotating disk (23) is provided with a moving groove (27) for the guide rod (25) to slide. The end face of the fixed disk (21) is provided with a sliding groove (29). A sliding block (210) is slidably connected inside the sliding groove (29). A connecting rod (211) is fixedly connected to the end face of the sliding block (210).

5. A cell resuscitation preservation box as described in claim 4, characterized in that: The end face of the rotating disk (23) is integrally formed with a first mounting block (212), the end face of the connecting rod (211) is fixedly connected with a first fixing block (213), and the outer wall of the first fixing block (213) is fixedly connected with an embedded block (215).

6. A cell resuscitation preservation box as described in claim 5, characterized in that: The outer wall of the first mounting block (212) is fixedly connected to a second fixing block (216), and the inner wall of the second fixing block (216) is fixedly connected to a card plate (214). The embedding block (215) can be embedded into the inner side of the card plate (214).

7. A cell resuscitation preservation box as described in claim 6, characterized in that: The end face of the fixed plate (21) is integrally formed with a second mounting block (218), and a spring (217) is fixedly connected to the outer wall of the first mounting block (212). The end of the spring (217) is fixedly connected to the end face of the second mounting block (218).

8. A cell resuscitation preservation box as described in claim 7, characterized in that: The upper end face of the test tube clip (12) is provided with a slot (28), and a water shield (219) is installed on the inner wall of the test tube clip (12). The end face of the water shield (219) is adapted to the slot (28).

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