An automated collection device in a protein purification process

Abstract

The utility model provides a kind of automatic collecting device in protein purification process, it is related to protein purification technical field, including temporary storage pipe, the inner wall between temporary storage pipe is close to the edge of top and is provided with layered pipe, the inner wall between layered pipe is close to the edge of top and is provided with discharge cavity, the inner wall between layered pipe is below discharge cavity and is provided with buffer cavity, the inside top surface of buffer cavity is close to the edge of one side and is provided with the feed inlet that is penetrated to the inside of discharge cavity, the inner wall between discharge cavity is close to the edge of bottom and is provided with annular limit slot, the both sides outer surface of temporary storage pipe is close to the edge of top and is fixed with mounting plate, the utility model can separate protein and tissue fluid, collect protein and tissue fluid respectively, and can continuously inject mixed liquid into its inside, improve the collection efficiency of protein.

Description

Technical Field

[0001] This utility model relates to the field of protein purification technology, and in particular to an automatic collection device in the protein purification process. Background Technology

[0002] Protein isolation and purification are widely used in biochemical research and are an important operational technique. To study a particular protein, it must first be purified from other proteins and non-protein molecules.

[0003] Currently, when purifying isolated proteins, the proteins and tissue fluid are mixed together. When the proteins float to the top, the tissue fluid below cannot be drained, resulting in excessive accumulation of tissue fluid and less protein floating on top, which affects the efficiency of protein purification. Utility Model Content

[0004] To address the problems in the prior art, this invention provides an automated collection device for the protein purification process.

[0005] To solve the above-mentioned technical problems, the basic concept of the technical solution adopted by this utility model is as follows: An automated collection device for protein purification includes a temporary storage tube. A layered tube is disposed between the inner walls of the temporary storage tube near its top edge. A discharge chamber is formed between the inner walls of the layered tubes near its top edge. A buffer chamber is formed between the inner walls of the layered tubes below the discharge chamber. An inlet is formed on the inner top surface of the buffer chamber near one side edge, extending into the discharge chamber. An annular limiting groove is formed between the inner walls of the discharge chamber near its bottom edge. Mounting plates are fixed to the outer surfaces of both sides of the temporary storage tube near their top edges.

[0006] Optionally, a conical disk is rotatably disposed between the inner walls of the annular limiting groove, and the top of the conical disk is conical.

[0007] Optionally, a through-hole is provided at the top of the conical disk near one side edge, and the through-hole is opposite to the feed port.

[0008] Optionally, a motor is fixed to the top of the layered tube, and the output end of the motor extends into the interior of the layered tube and is fixed to the top of the conical disk.

[0009] Optionally, one side of the layered tube is connected to a discharge pipe that extends into the discharge chamber, and the discharge pipe is located above the conical disc.

[0010] Optionally, a return pipe is fixedly connected to one side of the temporary storage tube, and a conical shroud is fixed between the inner walls of the temporary storage tube, with the top of the conical shroud extending obliquely toward the middle part of the temporary storage tube.

[0011] Optionally, the bottom of the temporary storage tube is connected to a feed tube, and the return tube is located above the conical shroud and the fixed part of the inner wall of the temporary storage tube.

[0012] By adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art. Of course, any product implementing the present invention does not necessarily need to achieve all of the following advantages at the same time: 1. In this invention, during the floating process of the protein inside the temporary storage tube, the conical septum causes the protein to rise from the middle part, while the tissue fluid inside the temporary storage tube will accumulate at the bottom of the temporary storage tube. The tissue fluid at the junction of the conical septum and the inner wall of the temporary storage tube has the lowest protein content, so it can be discharged through the reflux tube.

[0013] In this invention, a motor drives a conical disc to rotate. Each time the conical disc rotates to the position where the through-hole and the feed inlet are opposite, the protein inside the buffer chamber can enter the discharge chamber. Inside the discharge chamber, the protein is guided to the edge by the inclined surface at the top of the conical disc and discharged. Attached Figure Description

[0014] The accompanying drawings described below are merely some embodiments. Those skilled in the art can obtain other drawings based on these drawings without any creative effort. In the drawings: Figure 1 This utility model provides a front-view three-dimensional structural diagram of an automatic collection device in the protein purification process; Figure 2 A bottom-view three-dimensional structural diagram of an automatic collection device in the protein purification process is provided for this utility model; Figure 3 This invention provides a cross-sectional three-dimensional structural diagram of an automatic collection device in the protein purification process; Figure 4 For utility model Figure 3 A magnified view of point A in the middle.

[0015] The attached diagram lists the components represented by each number as follows: 1. Temporary storage pipe; 2. Layered pipe; 3. Motor; 4. Mounting plate; 5. Discharge pipe; 6. Return pipe; 7. Feed pipe; 8. Conical shroud; 9. Buffer chamber; 10. Discharge chamber; 11. Annular limiting groove; 12. Conical disc; 13. Through port; 14. Feed port.

[0016] It should be noted that these accompanying drawings and textual descriptions are not intended to limit the scope of the present invention in any way, but rather to illustrate the concept of the present invention to those skilled in the art by referring to specific embodiments. Detailed Implementation

[0017] To make the aforementioned objectives, features, and advantages of this solution more apparent and understandable, the specific embodiments of this solution are described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of this solution. However, this solution can be implemented in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of this solution. Therefore, this solution is not limited to the specific embodiments disclosed below.

[0018] In the description of this solution, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential," etc., indicating the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, are only for the convenience of describing this solution and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this solution. The terms "first" and "second" are used to distinguish one element from another and do not have sequential or importance. Furthermore, in the following description, when referring to the accompanying drawings, the same reference numerals in different drawings indicate the same or similar elements, which will not be repeated here.

[0019] In this solution, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this solution based on the specific circumstances.

[0020] According to some embodiments of this solution, an automated collection device for protein purification is provided, with reference to... Figures 1-4 As shown, the automatic collection device in the protein purification process includes a temporary storage tube 1. A layered tube 2 is provided between the inner walls of the temporary storage tube 1 near the top edge. A discharge chamber 10 is provided between the inner walls of the layered tube 2 near the top edge. A buffer chamber 9 is provided between the inner walls of the layered tube 2 below the discharge chamber 10. An inlet 14 is provided on the inner top surface of the buffer chamber 9 near one side edge, penetrating into the discharge chamber 10. An annular limiting groove 11 is provided between the inner walls of the discharge chamber 10 near the bottom edge. Mounting plates 4 are fixed on both outer surfaces of the temporary storage tube 1 near the top edge.

[0021] Please see Figures 1-4 A conical disk 12 is rotatably disposed between the inner walls of the annular limiting groove 11, and the top of the conical disk 12 is conical.

[0022] A through-hole 13 is provided at the top of the conical disk 12 near one side edge, and the through-hole 13 is opposite to the feed port 14.

[0023] A motor 3 is fixed to the top of the layered tube 2. The output end of the motor 3 extends into the interior of the layered tube 2 and is fixed to the top of the conical disk 12.

[0024] One side of the layered pipe 2 is connected to a discharge pipe 5 that extends into the discharge chamber 10, and the discharge pipe 5 is located above the conical disk 12.

[0025] In addition, a return pipe 6 is fixedly connected to one side of the temporary storage tube 1, and a conical shroud 8 is fixed between the inner walls of the temporary storage tube 1. The top of the conical shroud 8 extends inclined toward the middle part of the temporary storage tube 1, and a feed pipe 7 is connected to the bottom of the temporary storage tube 1. The return pipe 6 is located above the conical shroud 8 and the fixed part of the inner wall of the temporary storage tube 1.

[0026] Through the above technical solution, the automatic collection device in the protein purification process provided by this solution introduces the mixture of centrifuged tissue fluid and protein into the interior of the temporary storage tube 1 through the feed tube 7. Inside the temporary storage tube 1, since the protein floats upward in the tissue fluid, the protein will float from the bottom to the top of the temporary storage tube 1. During the floating process, the protein is pushed upward from the middle part by the conical septum 8. The tissue fluid inside the temporary storage tube 1 will accumulate at the bottom of the temporary storage tube 1. The tissue fluid at the junction of the conical septum 8 and the inner wall of the temporary storage tube 1 has the least protein content, so it can be discharged through the reflux tube 6. In this way, the mixture is continuously introduced into the temporary storage tube 1. After the protein enters the buffer chamber 9 and docks, the conical disk 12 is rotated by the motor 3. Each time the conical disk 12 rotates to the position where the through port 13 and the feed port 14 are opposite, the protein inside the buffer chamber 9 can enter the discharge chamber 10. Inside the discharge chamber 10, the protein is guided to the edge by the inclined surface at the top of the conical disk 12, so that it is discharged and collected through the discharge pipe 5.

[0027] The preferred embodiments of this solution have been described in detail above with reference to the accompanying drawings. However, this solution is not limited to the specific details in the above embodiments. Within the scope of the technical concept of this solution, various simple modifications can be made to the technical solution, and these simple modifications all fall within the protection scope of this solution.

[0028] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable way without contradiction. In order to avoid unnecessary repetition, this solution will not describe the various possible combinations separately.

[0029] Furthermore, various implementations of this solution can be combined in any way, as long as they do not violate the spirit of this solution, they should also be regarded as the content disclosed in this solution.

Claims

1. An automated collection device for protein purification, comprising a temporary storage tube (1), characterized in that: A layered tube (2) is provided between the inner walls of the temporary storage tube (1) near the top edge. A discharge cavity (10) is provided between the inner walls of the layered tube (2) near the top edge. A buffer cavity (9) is provided between the inner walls of the layered tube (2) below the discharge cavity (10). An inlet (14) is provided on the inner top surface of the buffer cavity (9) near one side edge, penetrating into the discharge cavity (10). An annular limiting groove (11) is provided between the inner walls of the discharge cavity (10) near the bottom edge. Mounting plates (4) are fixed on both outer surfaces of the temporary storage tube (1) near the top edge.

2. The automated collection device for protein purification according to claim 1, characterized in that: A conical disk (12) is rotatably disposed between the inner walls of the annular limiting groove (11), and the top of the conical disk (12) is conical.

3. The automated collection device for protein purification according to claim 2, characterized in that: The cone-shaped disk (12) has a through-hole (13) at the top near one side edge, and the through-hole (13) is opposite to the feed port (14).

4. An automated collection device for protein purification according to claim 3, characterized in that: A motor (3) is fixed to the top of the layered tube (2). The output end of the motor (3) extends into the interior of the layered tube (2) and is fixed to the top of the conical disk (12).

5. An automated collection device for protein purification according to claim 4, characterized in that: One side of the layered tube (2) is connected to a discharge pipe (5) that extends into the discharge chamber (10), and the discharge pipe (5) is located above the conical disc (12).

6. An automated collection device for protein purification according to claim 5, characterized in that: One side of the temporary storage tube (1) is fixedly connected to a return tube (6), and a conical septum (8) is fixed between the inner walls of the temporary storage tube (1). The top of the conical septum (8) extends obliquely toward the middle part of the temporary storage tube (1).

7. An automated collection device for protein purification according to claim 6, characterized in that: The bottom of the temporary storage tube (1) is connected to the feed tube (7), and the return tube (6) is located above the conical shroud (8) and the fixed part of the inner wall of the temporary storage tube (1).

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