A mixing device for polypeptide synthesis

Abstract

The utility model relates to polypeptide technical field, concretely relates to a polypeptide synthesis is with mixing device, including mixing bucket body, the bottom fixed mounting of mixing bucket body has the mounting bracket, the top fixed mounting of mixing bucket body has the placing box, the top intercommunication of mixing bucket body has the feed pipe, the inside of placing box is provided with stirring subassembly, the inside of feed pipe is provided with filter component, stirring subassembly includes stirring rod and stirring vane, stirring vane fixed mounting is at the stirring rod surface, and the bottom of stirring rod penetrates to the inside of mixing bucket body and and mixing bucket body swing installation. The utility model starts motor, through the cooperation of multiple groups of bevel gears transmission, drives stirring vane high -efficient rotation, and the mixing uniformity of material in mixing bucket is greatly promoted, and simultaneously transmission structure synchronous drive fan blade rotation, can be for the inside of placing box and mixing bucket top effective ventilation heat dissipation, can also timely blow away the dust on the surface of transmission part, guarantees equipment stable operation.

Description

Technical Field

[0001] This utility model relates to the field of polypeptide technology, specifically a mixing device for polypeptide synthesis. Background Technology

[0002] Polypeptides are compounds formed by two or more amino acids linked by peptide bonds. They are intermediate products of protein hydrolysis. Generally, compounds formed by the dehydration condensation of 10-100 amino acid molecules are called polypeptides. Their molecular weight is less than 10,000 Da. They can pass through semipermeable membranes and are not precipitated by trichloroacetic acid and ammonium sulfate. Some literature also refers to peptides composed of 2-10 amino acids as oligopeptides, peptides composed of 10-50 amino acids as polypeptides, and peptides composed of more than 50 amino acids as proteins.

[0003] The existing publication number CN220071653U discloses a mixing device for peptide synthesis, including a reaction vessel, an upper cover, a lower cover, and a housing. The device is characterized by a motor housing located above the housing, housing a motor, and a stirring rod inside the reaction vessel. One end of the stirring rod extends into the lower part of the reaction vessel, and the other end passes through the upper cover and extends into the motor housing, connecting to the motor output shaft for transmission. The stirring rod is rotatably connected to the upper cover. A stirring paddle is located at the lower end of the stirring rod, and a rotating blade is fitted around the middle of the stirring rod and fixedly connected to it. Multiple liquid inlet pipes are located above the upper cover, each connected to a flow sensor and a liquid inlet valve. The upper cover has a feed inlet. This application can sweep some reaction materials adhering to the reaction vessel wall back into the reaction solution during the reaction process, allowing for complete reaction. Simultaneously, it can automatically control quantitative liquid addition, simplifying the operation for staff.

[0004] While the aforementioned patents can prevent uneven mixing of peptides during the mixing process, the raw materials used in peptide synthesis may be mixed with small amounts of mechanical impurities, dust, or agglomerated particles during storage or transportation. Currently, most existing methods directly inject the raw materials into the mixing tank. After the impurities enter the reaction system with the materials, they will not only affect the purity of the peptide synthesis, but may also wear down the stirring components, block the discharge channel, and increase equipment maintenance costs. Therefore, a mixing device for peptide synthesis is proposed to solve the problems mentioned above. Utility Model Content

[0005] To address the shortcomings of existing technologies, this invention provides a mixing device for peptide synthesis, which can solve the problem that raw materials used in peptide synthesis may be mixed with small amounts of mechanical impurities, dust, or agglomerated particles during storage or transportation. Currently, most existing methods directly inject raw materials into the mixing tank. After impurities enter the reaction system with the materials, they not only affect the purity of peptide synthesis but may also wear down the stirring components, block the discharge channel, and increase equipment maintenance costs.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a mixing device for polypeptide synthesis, comprising a mixing tank body, an mounting frame fixedly installed at the bottom of the mixing tank body, a placement box fixedly installed at the top of the mixing tank body, a feed pipe connected to the top of the mixing tank body, a stirring assembly disposed inside the placement box, and a filter assembly disposed inside the feed pipe; The stirring assembly includes a stirring rod and stirring blades. The stirring blades are fixedly installed on the surface of the stirring rod, and the bottom of the stirring rod extends through the interior of the mixing tank body and is movably installed with the mixing tank body. The filter assembly includes a filter plate, and a baffle is fixedly installed inside the feed pipe, with the bottom of the filter plate and the top of the baffle in contact.

[0007] Preferably, the stirring assembly further includes a motor, the bottom of which is fixedly installed on the bottom of the mixing tank body and the top of which is fixedly installed on the top of the mixing tank body. The output end of the motor extends through the interior of the placement box and is fixedly installed with a first bevel gear. The top of the stirring rod is fixedly installed with a second bevel gear, and the first bevel gear and the second bevel gear mesh.

[0008] Preferably, the filter assembly further includes a square box, which is fixedly installed at the bottom of the baffle. A fixing rod is rotatably installed at the bottom of the inner wall of the square box. The top of the fixing rod extends through to the top of the filter plate. A cleaning plate is movably installed at the top of the fixing rod, and the bottom of the cleaning plate contacts the top of the filter plate.

[0009] Preferably, the placement box has a third bevel gear inside, which meshes with the second bevel gear. A connecting rod is fixedly installed at the right end of the third bevel gear. The right end of the connecting rod passes through the inside of the square box and is fixedly installed with a fourth bevel gear. A fifth bevel gear is fixedly installed on the surface of the fixing rod, and the fourth and fifth bevel gears mesh.

[0010] Preferably, the placement box has two sets of rotating rods inside, and two sets of fixing blocks are fixedly installed on the top of the second bevel gear. The fixing blocks and rotating rods are rotatably installed, and fan blades are fixedly installed on the outer end of the rotating rods.

[0011] Preferably, the top of the stirring rod and the top of the inner wall of the placement box are rotatably mounted, a sixth bevel gear is fixedly mounted on the top of the surface of the stirring rod, and a seventh bevel gear is fixedly mounted on the surface of the rotating rod, with the sixth bevel gear and the seventh bevel gear meshing.

[0012] Preferably, the front of the placement box is provided with glass, and the four corners of the front of the glass are detachably installed with the placement box by screws.

[0013] Compared with the prior art, the present invention provides a mixing device for polypeptide synthesis, which has the following advantages: 1. After the motor is started, the stirring blades are driven to rotate efficiently through the coordinated transmission of multiple sets of bevel gears, which greatly improves the mixing uniformity of the materials in the mixing tank. At the same time, the transmission structure drives the fan blades to rotate synchronously, which can effectively ventilate and dissipate heat for the inside of the box and the top of the mixing tank, and can also blow away the dust on the surface of the transmission components in time, ensuring the stable operation of the equipment.

[0014] 2. To prevent the filter plate from clogging due to the accumulation of impurities and to ensure smooth feeding, the transmission structure composed of bevel gears and connecting rods drives the cleaning plate to rotate, efficiently scraping away impurities on the filter plate and ensuring that the filtration channel is always unobstructed. The glass on the front of the box allows for a direct observation of the working status of the internal stirring and filtration components. The glass can be removed by simply unscrewing the four corner screws, making it convenient to maintain the internal transmission components. Attached Figure Description

[0015] Figure 1 This is a front view of the mixing tank body of this utility model; Figure 2 This is a cross-sectional view of the mixing tank body of this utility model; Figure 3 This is a cross-sectional view of the placement box of this utility model; Figure 4 This utility model Figure 3 Enlarged structural diagram at point A in the middle; Figure 5 This utility model Figure 3 Enlarged structural diagram at point B.

[0016] In the diagram: 1. Mixing tank body; 2. Mounting frame; 3. Placement box; 4. Feed pipe; 5. Stirring assembly; 51. Stirring rod; 52. Stirring blade; 53. Motor; 54. First bevel gear; 55. Second bevel gear; 6. Filter assembly; 61. Filter plate; 62. Baffle; 63. Square box; 64. Fixing rod; 65. Cleaning plate; 66. Third bevel gear; 67. Connecting rod; 68. Fourth bevel gear; 69. Fifth bevel gear; 7. Rotating rod; 8. Fixing block; 9. Fan blade; 10. Sixth bevel gear; 11. Seventh bevel gear; 12. Glass. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0018] Example: Please see Figure 1 - Figure 5 A mixing device for polypeptide synthesis in this embodiment includes a mixing tank body 1, a mounting frame 2 fixedly installed at the bottom of the mixing tank body 1, a placement box 3 fixedly installed at the top of the mixing tank body 1, a feed pipe 4 connected to the top of the mixing tank body 1, a stirring assembly 5 disposed inside the placement box 3, and a filter assembly 6 disposed inside the feed pipe 4. The stirring assembly 5 includes a stirring rod 51 and a stirring blade 52. The stirring blade 52 is fixedly installed on the surface of the stirring rod 51. The bottom of the stirring rod 51 extends through the interior of the mixing tank body 1 and is movably installed with the mixing tank body 1. The filter assembly 6 includes a filter plate 61, and a baffle 62 is fixedly installed inside the feed pipe 4. The bottom of the filter plate 61 and the top of the baffle 62 are in contact. When in use, when it is necessary to add the materials required for peptide synthesis into the mixing tank body 1, the materials are injected through the top feed pipe 4. The filter plate 61 inside the feed pipe 4 is kept horizontal under the support of the baffle 62, which can filter the impurities in the materials and prevent the impurities from entering the mixing tank body 1 and affecting the quality of peptide synthesis. At the same time, the stirring rod 51 drives the stirring blades 52 on the surface to rotate inside the mixing tank body 1, and performs preliminary mixing and stirring of the filtered materials, laying the foundation for subsequent uniform mixing.

[0019] The stirring assembly 5 also includes a motor 53, which is fixedly installed at the bottom and top of the mixing tank body 1. The output end of the motor 53 extends into the interior of the placement box 3 and is fixedly installed with a first bevel gear 54. A second bevel gear 55 is fixedly installed at the top of the stirring rod 51. The first bevel gear 54 and the second bevel gear 55 mesh with each other. The interior of the placement box 3 is provided with two sets of rotating rods 7. The top of the second bevel gear 55 is fixedly installed with two sets of fixing blocks 8. The fixing blocks 8 and the rotating rods 7 are rotatably installed. The outer end of the rotating rods 7 is fixedly installed with fan blades 9. The top of the stirring rod 51 and the top of the inner wall of the placement box 3 are rotatably mounted. A sixth bevel gear 10 is fixedly mounted on the top of the surface of the stirring rod 51, and a seventh bevel gear 11 is fixedly mounted on the surface of the rotating rod 7. The sixth bevel gear 10 and the seventh bevel gear 11 mesh.

[0020] When in use, if it is necessary to enhance the mixing effect of materials and realize auxiliary functions, the motor 53 is started. The output end of the motor 53 drives the first bevel gear 54 to rotate. The rotation of the first bevel gear 54 drives the second bevel gear 55 to rotate. The rotation of the second bevel gear 55 drives the stirring rod 51 to rotate. The rotation of the stirring rod 51 drives the stirring blade 52 to rotate, thereby improving the mixing uniformity of materials in the mixing barrel body 1. At the same time, the fixing block 8 at the top of the second bevel gear 55 rotates together with the second bevel gear 55. The rotation of the fixing block 8 drives the rotating rod 7 to rotate. The rotation of the rotating rod 7 drives the sixth bevel gear 10 to rotate. The rotation of the sixth bevel gear 10 drives the seventh bevel gear 11 to rotate. The fan blade 9 at the outer end of the rotating rod 7 rotates accordingly, which can ventilate and dissipate heat inside the placement box 3 and the top area of ​​the mixing barrel body 1. It can also blow away the dust on the transmission components inside the placement box 3.

[0021] Please see Figure 1 - Figure 5 The filter assembly 6 also includes a square box 63, which is fixedly installed at the bottom of the baffle 62. A fixing rod 64 is rotatably installed at the bottom of the inner wall of the square box 63. The top of the fixing rod 64 extends through to the top of the filter plate 61. A cleaning plate 65 is movably installed at the top of the fixing rod 64. The bottom of the cleaning plate 65 contacts the top of the filter plate 61. The interior of the placement box 3 is provided with a third bevel gear 66, which meshes with the second bevel gear 55. A connecting rod 67 is fixedly installed on the right end of the third bevel gear 66. The right end of the connecting rod 67 passes through the interior of the square box 63 and is fixedly installed with a fourth bevel gear 68. A fifth bevel gear 69 is fixedly installed on the surface of the fixing rod 64. The fourth bevel gear 68 and the fifth bevel gear 69 mesh. The front of the placement box 3 is provided with glass 12, and the four corners of the front of the glass 12 are detachable from the placement box 3 by screws.

[0022] In use, when it is necessary to prevent the filter plate 61 of the filter assembly 6 from being clogged by impurities and to ensure smooth feeding, the rotation of the second bevel gear 55 will drive the rotation of the third bevel gear 66. The rotation of the third bevel gear 66 will drive the rotation of the connecting rod 67. The rotation of the connecting rod 67 will drive the rotation of the fourth bevel gear 68. The rotation of the fourth bevel gear 68 will drive the rotation of the fixing rod 64 and the fifth bevel gear 69. The rotation of the fixing rod 64 will drive the rotation of the cleaning plate 65. The rotation of the cleaning plate 65 can scrape and clean the impurities on the filter plate 61, ensuring that the filter channel of the filter plate 61 is unobstructed. The working status of the internal stirring assembly 5 and the filter assembly 6 can be observed through the glass 12 on the front side of the placement box 3. When it is necessary to maintain the transmission components inside the placement box 3, simply remove the screws at the four corners to remove the glass 12 from the placement box 3.

[0023] The working principle of the above embodiments is as follows: When it is needed to add the materials required for peptide synthesis into the mixing tank body 1, the materials are injected from the top feed pipe 4. During the feeding process, the filter plate 61 in the feed pipe 4 is kept horizontal under the support of the baffle 62 to filter the impurities in the materials and prevent the impurities from entering the mixing tank body 1 and affecting the synthesis quality. Then the motor 53 is started. The output end of the motor 53 drives the first bevel gear 54 to rotate. The rotation of the first bevel gear 54 drives the second bevel gear 55 to rotate. The rotation of the second bevel gear 55 drives the stirring rod 51 and the stirring blade 52 to rotate, thereby stirring and mixing the filtered material and improving the uniformity of the material. At this time, when the second bevel gear 55 rotates, it drives the meshing third bevel gear 66 to rotate. The rotation of the third bevel gear 66 drives the connecting rod 67 and the fourth bevel gear 68 to rotate. The rotation of the fourth bevel gear 68 drives the fifth bevel gear 69 and the fixed rod 64 to rotate. At this time, the cleaning plate 65 at the top of the fixed rod 64 rotates with it, thereby scraping off the impurities accumulated on the filter plate 61 and ensuring that the filter channel is unobstructed. When the stirring rod 51 rotates, it will also drive the sixth bevel gear 10 to rotate. The rotation of the sixth bevel gear 10 will drive the rotating rod 7 and the seventh bevel gear 11 to rotate, and will also drive the fan blade 9 to rotate. When the fan blade 9 rotates, it can not only ventilate and dissipate heat in the top area of ​​the mixing tank body 1, but also blow away the dust on the transmission components inside the placement box 3.

[0024] The installation, connection, or setting methods disclosed in this embodiment are all common mechanical connection methods. As long as they can achieve their beneficial effects, they can be implemented. Therefore, this embodiment will not elaborate on their specific structural composition and working principle.

[0025] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A mixing apparatus for polypeptide synthesis, characterized in that: The mixture includes a mixing tank body (1), a mounting bracket (2) is fixedly installed at the bottom of the mixing tank body (1), a placement box (3) is fixedly installed at the top of the mixing tank body (1), a feed pipe (4) is connected to the top of the mixing tank body (1), a stirring assembly (5) is provided inside the placement box (3), and a filter assembly (6) is provided inside the feed pipe (4). The stirring assembly (5) includes a stirring rod (51) and a stirring blade (52). The stirring blade (52) is fixedly installed on the surface of the stirring rod (51). The bottom of the stirring rod (51) extends into the interior of the mixing tank body (1) and is movably installed with the mixing tank body (1). The filter assembly (6) includes a filter plate (61), and a baffle (62) is fixedly installed inside the feed pipe (4). The bottom of the filter plate (61) and the top of the baffle (62) are in contact.

2. The mixing apparatus for polypeptide synthesis according to claim 1, characterized in that: The stirring assembly (5) also includes a motor (53), the bottom of the motor (53) and the top of the mixing tank body (1) are fixedly installed, the output end of the motor (53) extends into the interior of the placement box (3) and is fixedly installed with a first bevel gear (54), and the top of the stirring rod (51) is fixedly installed with a second bevel gear (55), and the first bevel gear (54) and the second bevel gear (55) mesh.

3. The mixing apparatus for polypeptide synthesis according to claim 1, characterized in that: The filter assembly (6) also includes a square box (63), which is fixedly installed at the bottom of the baffle (62). A fixing rod (64) is rotatably installed at the bottom of the inner wall of the square box (63). The top of the fixing rod (64) extends through to the top of the filter plate (61). A cleaning plate (65) is movably installed at the top of the fixing rod (64). The bottom of the cleaning plate (65) contacts the top of the filter plate (61).

4. The mixing apparatus for polypeptide synthesis according to claim 3, characterized in that: The placement box (3) is provided with a third bevel gear (66) inside. The third bevel gear (66) meshes with the second bevel gear (55). A connecting rod (67) is fixedly installed on the right end of the third bevel gear (66). The right end of the connecting rod (67) passes through the inside of the square box (63) and is fixedly installed with a fourth bevel gear (68). A fifth bevel gear (69) is fixedly installed on the surface of the fixing rod (64). The fourth bevel gear (68) and the fifth bevel gear (69) mesh.

5. A mixing apparatus for polypeptide synthesis according to claim 2, characterized in that: The placement box (3) is provided with two sets of rotating rods (7) inside. Two sets of fixing blocks (8) are fixedly installed on the top of the second bevel gear (55). The fixing blocks (8) and the rotating rods (7) are rotatably installed. The outer end of the rotating rods (7) is fixedly installed with fan blades (9).

6. The mixing apparatus for polypeptide synthesis according to claim 5, characterized in that: The top of the stirring rod (51) and the top of the inner wall of the placement box (3) are rotatably mounted. A sixth bevel gear (10) is fixedly mounted on the top of the surface of the stirring rod (51), and a seventh bevel gear (11) is fixedly mounted on the surface of the rotating rod (7). The sixth bevel gear (10) and the seventh bevel gear (11) mesh.

7. The mixing apparatus for polypeptide synthesis according to claim 1, characterized in that: The front side of the placement box (3) is provided with glass (12), and the four corners of the front of the glass (12) are detachably installed with screws and the placement box (3).

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