A detection kit for detecting ganoderic acid A content

Abstract

The utility model relates to detection kit technical field, concretely is a detection kit for detecting ganoderic acid A content. The utility model discloses reagent box body, the cavity of reagent box body places the rack, the surface of rack is equipped with two groups of placement grooves, two groups of placement grooves are equipped with four and five respectively, the cavity of a group of placement grooves is slidably connected with enzyme marker, anti ganoderic acid A's monoclonal antibody solution, color developing agent and stop solution respectively, the cavity of another group of placement grooves is slidably connected with different concentrations of ganoderic acid A standard solution, the surface of rack is coated with the enzyme label plate of ganoderic acid A antigen or goat anti mouse secondary antibody. Solveed the problem that the staff needs to observe and compare the content of each reagent bottle in ganoderic acid A kit, the staff needs to take out the reagent bottle on the reagent box rack in turn and compare, the operation is extremely tedious, and the staff's work load is increased.

Description

Technical Field

[0001] This utility model relates to the field of detection kit technology, and in particular to a detection kit for detecting the content of ganoderic acid A. Background Technology

[0002] This test kit is specifically designed for detecting the content of ganoderic acid A in samples such as traditional Chinese medicine preparations, medicinal herbs, health products, and cosmetics. It employs advanced technology and a sensitive detection method to rapidly and accurately determine the content of ganoderic acid A. The kit is easy to operate and use, providing reliable data support for the quality control and research of traditional Chinese medicinal materials. By using this kit, the content of ganoderic acid A in samples such as traditional Chinese medicine preparations, medicinal herbs, health products, and cosmetics can be effectively evaluated, ensuring the quality and efficacy of these products. Ganoderic acid is a triterpenoid compound found in Ganoderma lucidum (reishi mushroom) and possesses certain biological activities, potentially offering some health benefits to humans.

[0003] Ganoderma lucidum acid A is mostly extracted using solvent extraction methods.

[0004] Principle: Based on the property that ganoderic acids are soluble in organic solvents, a suitable organic solvent is selected to dissolve them from the Ganoderma lucidum raw material. Procedure: After pulverizing the Ganoderma lucidum fruiting body or mycelium, an appropriate amount of organic solvent, such as ethanol or methanol, is added. The mixture is then stirred or soaked under specific temperature and time conditions for extraction. The extract is then filtered and concentrated to obtain the ganoderic acid extract. Advantages: Simple operation, low cost, and relatively high extraction efficiency.

[0005] The inventors believe that the following drawbacks often exist: when staff need to observe and compare the content of each reagent bottle in the Ganoderma lucidum acid A kit, they need to take out the reagent bottles from the kit rack one by one for comparison, which is extremely cumbersome and increases the workload of staff; therefore, in order to address the above problems, a detection kit for detecting the content of Ganoderma lucidum acid A in Chinese medicinal materials is proposed. Utility Model Content

[0006] The purpose of this invention is to address the shortcomings of existing technologies where staff need to take out reagent bottles from the reagent rack one by one for comparison when observing and comparing the content of ganoderic acid A, which is extremely cumbersome and increases the workload of staff. Therefore, this invention proposes a detection kit for detecting the content of ganoderic acid A.

[0007] To achieve the above objectives, the present invention adopts the following technical solution: a detection kit for detecting ganoderic acid A content, comprising a kit body, a placement rack placed in the inner cavity of the kit body, two sets of placement slots with four and five slots respectively on the surface of the placement rack, an enzyme label, an anti-ganoderic acid A monoclonal antibody solution, a chromogenic agent and a stop solution being slidably connected to the inner cavity of one set of placement slots, and ganoderic acid A standard solutions of different concentrations being slidably connected to the inner cavity of the other set of placement slots, an enzyme-labeled plate coated with ganoderic acid A antigen or goat anti-mouse secondary antibody being adhered to the surface of the placement rack, a sliding groove being formed on both sides of the kit body, a push plate being slidably connected to the inner surface of the sliding groove of the kit body, the push plate being fixedly connected to the side wall of the placement rack, two support plates being fixedly connected to both sides of the kit body, a fixing rod being slidably connected to the support plate, a fixing groove being formed on the side wall of the push plate, the arc surface of the fixing rod being slidably connected to the fixing groove of the push plate, and a pull block being fixedly connected to one end of the fixing rod.

[0008] The aforementioned components achieve the following effects: the main body of the Chinese herbal medicine reagent kit can detect samples containing this ingredient, such as prepared Chinese medicines, Chinese herbal medicines, health products, and cosmetics. It facilitates the observation of each reagent bottle inside the Ganoderma lucidum A reagent kit, avoiding the inconvenience of staff removing the internal rack when observing the reagent bottles. This avoids the situation where staff need to remove each reagent bottle one by one, increasing the space occupied by the reagent bottles and the workload of staff, thus improving the practicality of the device.

[0009] Preferably, the arc surface of the fixing rod is fitted with a first spring, and the two ends of the first spring are fixedly connected to the pull block and the support plate, respectively.

[0010] The effect achieved by the above components is that, under the action of the first spring, the placement rack is automatically fixed after being lifted, thus improving the practicality of the device.

[0011] Preferably, a fixing plate is adhered to the inner surface of the reagent kit body, and three base plates are fixedly connected to the surface of the fixing plate. Two buffer springs are fixedly connected to the surface of each of the three base plates, and buffer plates are fixedly connected to the surface of each buffer spring.

[0012] The aforementioned components achieve the following effect: they buffer the slide of the rack, preventing the reagent bottles in the Ganoderma lucidum acid A reagent kit from shaking and improving the practicality of the device.

[0013] Preferably, a telescopic rod is fixedly connected to the surface of the base plate, and one end of the telescopic rod is fixedly connected to the buffer plate.

[0014] The effect achieved by the above components is to prevent the buffer spring from deforming after long-term use, which would prevent the buffer plate from effectively cushioning the placement rack, thus improving the practicality of the device.

[0015] Preferably, a U-shaped plate is fixedly connected to the side wall of the reagent kit body, a rotating rod is rotatably connected inside the U-shaped plate, a cover plate is fixedly connected to the arc surface of the rotating rod, and a threaded rod is threadedly inserted into the cover plate, with one end of the threaded rod threaded into the reagent kit body.

[0016] The effect achieved by the above components is to seal the reagent kit body, preventing other impurities from entering the Ganoderma lucidum acid A reagent kit and thus affecting the reagent detection within the Ganoderma lucidum acid A reagent kit, thereby improving the practicality of the device.

[0017] Preferably, both the inner surface of the cover plate and the surface of the buffer plate are fixedly connected with soft pads, and the soft pads are made of rubber.

[0018] The aforementioned components achieve the following effect: they securely protect the reagent bottles within the Ganoderma lucidum acid A reagent kit, preventing shaking and collisions of the reagent bottles inside the kit body during transport by staff, thus improving the practicality of the device.

[0019] Preferably, a handle is fixedly connected to the surface of the cover plate.

[0020] The effect achieved by the above-mentioned components is that the handle facilitates the carrying and transportation of the reagent kit by staff, thereby improving the practicality of the device.

[0021] In summary, the beneficial effects of this utility model are as follows:

[0022] This invention facilitates the observation of reagent bottles inside the reagent kit body, avoiding the situation where staff need to remove the rack inside the reagent kit body when they need to observe the reagent bottles, which would increase the space occupied by the reagent bottles and the workload of the staff. This improves the practicality of the device. Attached Figure Description

[0023] Figure 1 is a three-dimensional structural schematic diagram of this utility model;

[0024] Figure 2 is a side view of this utility model;

[0025] Figure 3 is an enlarged view of point A in Figure 1 of this utility model;

[0026] Figure 4 is a schematic diagram of the structure of the placement rack in this utility model.

[0027] Legend: 1. Reagent kit body; 2. Placement rack; 3. Push plate; 4. Support plate; 5. Fixing rod; 6. Pull block; 7. First spring; 8. Enzyme label; 9. Monoclonal antibody solution against ganoderic acid A; 10. Chromogenic reagent; 11. Stop solution; 12. Standard solutions of ganoderic acid A at different concentrations; 13. U-shaped plate; 14. Rotating rod; 15. Cover plate; 16. Threaded rod; 17. Handle; 18. Soft pad; 19. Base plate; 20. Buffer spring; 21. Buffer plate; 22. Telescopic rod; 23. Enzyme-labeled plate coated with ganoderic acid A antigen or goat anti-mouse secondary antibody. Detailed Implementation

[0028] Refer to Figure 1- Figure 4As shown, this utility model provides a technical solution: a detection kit for detecting the content of ganoderic acid A, including a kit body 1, a placement rack 2 placed in the inner cavity of the kit body 1, two sets of placement slots with four and five slots respectively on the surface of the placement rack 2, an enzyme label 8, a monoclonal antibody solution 9 against ganoderic acid A, a chromogenic agent 10 and a stop solution 11 slidably connected to the inner cavity of one set of placement slots, and ganoderic acid A standard solutions 12 of different concentrations slidably connected to the inner cavity of the other set of placement slots, an enzyme-labeled plate 23 coated with ganoderic acid A antigen or goat anti-mouse secondary antibody adhered to the surface of the placement rack 2, a sliding groove on both sides of the kit body 1, a push plate 3 slidably connected to the inner surface of the sliding groove of the kit body 1, the push plate 3 being fixedly connected to the side wall of the placement rack 2, two support plates 4 fixedly connected to both sides of the kit body 1, a fixing rod 5 slidably connected in the support plate 4, a fixing groove on the side wall of the push plate 3, and the arc surface of the fixing rod 5 being flush with the push plate 3. The fixed groove is slidably connected, and a pull block 6 is fixedly connected to one end of the fixed rod 5, which facilitates the observation of the reagent bottles inside the reagent kit body 1. This avoids the situation where staff need to remove the placement rack 2 inside the reagent kit body 1 when they need to observe the reagent bottles, which would otherwise be inconvenient and would require staff to remove the reagent bottles one by one, increasing the space occupied by the reagent bottles and the workload of the staff. This improves the practicality of the device. A first spring 7 is sleeved on the arc surface of the fixed rod 5. The two ends of the first spring 7 are fixedly connected to the pull block 6 and the support plate 4, respectively. Under the action of the first spring 7, the placement rack 2 is automatically fixed after being lifted, improving the practicality of the device. A fixing plate is adhered to the inner surface of the reagent kit body 1. Three base plates 19 are fixedly connected to the fixed surface. Two buffer springs 20 are fixedly connected to the surface of each of the three base plates 19. Buffer plates 21 are fixedly connected to the surface of the buffer springs 20, which achieves the effect of buffering when the placement rack 2 slides down, preventing the reagent bottles inside the placement rack 2 from shaking, thus improving the practicality of the device. A telescopic rod 22 is fixedly connected to the surface of the reagent kit 20. One end of the telescopic rod 22 is fixedly connected to the buffer plate 21, which prevents the buffer spring 20 from deforming over long-term use and thus prevents the buffer plate 21 from failing to effectively buffer the rack 2, thereby improving the practicality of the device. A U-shaped plate 13 is fixedly connected to the side wall of the reagent kit body 1. A rotating rod 14 is rotatably connected inside the U-shaped plate 13. A cover plate 15 is fixedly connected to the arc surface of the rotating rod 14. A threaded rod 16 is threaded into the cover plate 15. One end of the threaded rod 16 is threaded into the reagent kit body 1, achieving a sealing effect on the reagent kit body 1 and preventing other impurities from entering the reagent kit, thereby affecting the detection of reagents inside the kit and improving the practicality of the device.Both the inner surface of the cover plate 15 and the surface of the buffer plate 21 are fixedly connected to soft pads 18. The soft pads 18 are made of rubber, effectively securing and protecting the test tubes. This prevents the test tubes inside the reagent kit 1 from shaking or colliding during transport, improving the device's practicality. A handle 17 is also fixedly connected to the surface of the cover plate 15, facilitating easy carrying and transport of the reagent kit 1, further enhancing the device's usability.

[0029] Working principle: Mass spectrometry is mostly used for the detection of ganoderic acid A content in samples such as traditional Chinese medicine, medicinal herbs, health products, and cosmetics. This can improve the accuracy and specificity of the detection. Before detection, the sample needs to be properly pretreated, such as extracted and purified. At the same time, the detection conditions need to be strictly controlled to ensure the accuracy and reliability of the detection results. When the staff needs to observe the test tube containing ganoderic acid A content in the reagent kit, the staff rotates the threaded rod inside the cover plate until one end of the threaded rod disengages from the surface of the reagent kit. This pushes the cover plate, causing the rotating rod to rotate inside the U-shaped plate, thereby opening the cover plate. Then, the staff pushes the push plate into the slide groove inside the reagent kit. The sliding mechanism works as follows: When the push plate slides to its highest point, it contacts the fixed rod, pressing it against the rod. The fixed rod is then stretched backward by the spring force. When the fixed groove on the push plate is at the same level as the fixed rod, the fixed rod automatically springs into the groove under the spring force. This facilitates observation of the test tubes inside the reagent kit, avoiding the inconvenience of removing the internal rack when staff need to observe the reagent bottles in the Ganoderma lucidum A reagent kit. This previously required staff to remove each reagent bottle sequentially, increasing the space occupied by the bottles and the workload for staff, thus improving the practicality of the device.

[0030] The specific operating procedures are as follows: Standard solutions of different concentrations of ganoderic acid A are packaged in brown translucent PE plastic bottles with black caps, each containing 10 ml / bottle, and are orange-red liquids; colorimetric reagents are packaged in black PE plastic bottles, each containing 25 ml / bottle, and are transparent and clear liquids; stop solutions are packaged in translucent white PE plastic bottles with yellow caps, each containing 25 ml / bottle, and are transparent and clear liquids; enzyme-labeled solutions are packaged in translucent white PE plastic bottles with blue caps, each containing 25 ml / bottle, and are transparent and clear liquids; monoclonal antibody solutions against ganoderic acid A are packaged in translucent white PE plastic bottles with green caps, each containing 25 ml / bottle, and are transparent and clear liquids.

[0031] By preparing specific monoclonal antibodies against small molecule compounds of traditional Chinese medicine, a simple, rapid, and sensitive immunoassay method for traditional Chinese medicine can be established. Among the immunoassay methods, ELISA is the most commonly used. ELISA has the characteristics of high specificity, simple operation, high sensitivity, and easy automation. It can be used not only for antibody determination but also for antigen determination in samples. Its basic principle is as follows: (1) Antigens or antibodies are physically adsorbed on the surface of a solid-phase carrier (an enzyme-labeled plate coated with ganoderic acid A antigen or goat anti-mouse secondary antibody) and maintain immunological activity; (2) Antigens or antibodies bind to enzymes to form enzyme conjugates and maintain immunological and enzyme activity; (3) Enzyme conjugates bind to the corresponding antigens or antibodies. When a substrate is added, a color reaction occurs. Since the intensity of the color of the reactant is proportional to the amount of the corresponding antigen or antibody in the sample, the test results can be displayed according to the degree of substrate color development.

[0032] The detection method is as follows:

[0033] (1) Preparation of cleaning solution: Dilute the 10-fold concentrated cleaning solution with deionized water at a volume ratio of 1:9 according to the required amount (e.g., take 10mL of 10-fold concentrated cleaning solution into 90mL of distilled water) and mix well before use.

[0034] (2) Take out the required number of enzyme-labeled strips coated with ganoderic acid A antigen or goat anti-mouse secondary antibody and insert them into the enzyme-labeled plate holder coated with ganoderic acid A antigen or goat anti-mouse secondary antibody. Record the position of each standard and sample solution. To reduce the fluctuation of the detection value, perform a two-well parallel experiment.

[0035] (3) Add 50 μL of standard / sample solution to the corresponding microwell, then add 50 μL of enzyme label / well and 50 μL of Ganoderma lucidum acid A monoclonal antibody working solution / well. Gently shake for 5 seconds to mix, cover the plate and place it in a 25°C dark environment for 15 min to react.

[0036] (4) Remove the cover film, shake off the liquid in the hole, add 300μL of cleaning solution per hole, wash 4 times, pour out the cleaning solution, and pat dry with absorbent paper.

[0037] (5) Add 100 μL of substrate developer per well, mix well, cover the plate and incubate at 25°C in the dark for 15 min.

[0038] (6) Add 100 μL of stop solution per well, set the microplate reader to 450 nm, and measure the OD value.

[0039] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

Claims

1. A detection kit for detecting the content of ganoderic acid A, comprising a kit body (1), characterized in that: The reagent kit body (1) contains a placement rack (2). The surface of the placement rack (2) has two sets of placement slots, with four and five slots respectively. The inner cavity of one set of placement slots is slidably connected to an enzyme label (8), a monoclonal antibody solution (9) against ganoderic acid A, a chromogenic agent (10), and a stop solution (11). The inner cavity of the other set of placement slots is slidably connected to standard solutions (12) of ganoderic acid A at different concentrations. The surface of the placement rack (2) is coated with ganoderic acid A. An enzyme-labeled plate (23) containing antigen or goat anti-mouse secondary antibody is provided. The kit body (1) has grooves on both sides. A push plate (3) is slidably connected to the inner surface of the groove of the kit body (1). The push plate (3) is fixedly connected to the side wall of the placement rack (2). Two support plates (4) are fixedly connected to both sides of the kit body (1). A fixing rod (5) is slidably connected inside the support plate (4). A fixing groove is provided on the side wall of the push plate (3). The arc surface of the fixing rod (5) is slidably connected to the fixing groove of the push plate (3). A pull block (6) is fixedly connected to one end of the fixing rod (5).

2. The detection kit for detecting ganoderic acid A content according to claim 1, characterized in that: The arc surface of the fixing rod (5) is fitted with a first spring (7), and the two ends of the first spring (7) are fixedly connected to the pull block (6) and the support plate (4) respectively.

3. The detection kit for detecting ganoderic acid A content according to claim 1, characterized in that: The inner surface of the reagent kit body (1) is attached with a fixing plate, and three base plates (19) are fixedly connected to the surface of the fixing plate. Two buffer springs (20) are fixedly connected to the surface of each of the three base plates (19). A buffer plate (21) is fixedly connected to the surface of the buffer springs (20). The buffer plate (21) is slidably connected to the placement rack (2).

4. The detection kit for detecting ganoderic acid A content according to claim 3, characterized in that: A telescopic rod (22) is fixedly connected to the surface of the base plate (19), and one end of the telescopic rod (22) is fixedly connected to the buffer plate (21).

5. The detection kit for detecting ganoderic acid A content according to claim 1, characterized in that: A U-shaped plate (13) is fixedly connected to the side wall of the reagent kit body (1). A rotating rod (14) is rotatably connected inside the U-shaped plate (13). A cover plate (15) is fixedly connected to the arc surface of the rotating rod (14). A threaded rod (16) is threadedly inserted inside the cover plate (15). One end of the threaded rod (16) is threadedly inserted into the reagent kit body (1).

6. The detection kit for detecting ganoderic acid A content according to claim 5, characterized in that: The inner surface of the cover plate (15) and the surface of the buffer plate (21) are both fixedly connected with soft pads (18), and the soft pads (18) are made of rubber.

7. The detection kit for detecting ganoderic acid A content according to claim 5, characterized in that: A handle (17) is fixedly connected to the surface of the cover plate (15).

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