Portable plant tissue sample cold storage box

Abstract

The utility model discloses a portable plant tissue sample refrigeration box relates to biological sample storage technical field, including heat preservation box, limit block, heat exchange plate, temperature sensor and controller component, adopt the heat exchange plate based on phase change material (PCM) as the cold source, can absorb a large amount of heat in the phase change process, make the temperature in the box long -time stable in phase change point vicinity, avoided the problem that the temperature in ordinary heat preservation box continued to rise, provided the constant low -temperature preservation environment for sample, and through copper heat exchange plate and fin increased heat exchange area, ensured that the cold distribution in the box was even, and heat exchange plate block, the core components such as branch plate were all the modular design of detachable, not only lightened the overall weight, conveniently carried, also made precooling, cleaning and maintenance more flexible and convenient, perfect fit the demand of field operation.

Description

Technical Field

[0001] This utility model relates to the field of biological sample storage technology, specifically a lightweight plant tissue sample cold storage box. Background Technology

[0002] In field research in botany, agronomy, ecology, and other related fields, it is often necessary to collect plant tissue samples (such as leaves, roots, and stems) and quickly transport them back to the laboratory at low temperatures to maintain their biological activity and prevent the degradation of biomolecules such as RNA, DNA, and proteins. Existing sample preservation methods have the following main shortcomings:

[0003] In fixed laboratory settings, ultra-low temperature freezers can be used for long-term storage, relying on traditional refrigerators or dry ice; for short-distance field transport, dry ice is a common choice. However, the former is immobile, while the latter is expensive, inconvenient to obtain, poses an explosion risk during sublimation, and its temperature is difficult to control precisely.

[0004] Ordinary insulated boxes have limited effectiveness. While common portable insulated boxes (such as foam boxes) are lightweight, they have short cooling times, uneven internal temperatures, and cannot be monitored in real time. After samples are placed inside, the temperature inside the box will continue to rise due to the intrusion of ambient heat, making it impossible to maintain a stable low-temperature environment. This poses a risk of deterioration for temperature-sensitive samples. Utility Model Content

[0005] (a) Technical problems to be solved To address the shortcomings of existing technologies, this invention provides a lightweight plant tissue sample cold storage box, which solves the aforementioned problems.

[0006] (0) Technical solution To achieve the above objectives, this utility model provides the following technical solution: a lightweight plant tissue sample cold storage box, comprising an insulated box, limiting blocks, a heat exchange plate, a temperature sensor, and a controller assembly. The top of the insulated box is sealed with a top cover. A support edge is provided in the middle of the inner wall of the insulated box to support the bottom edge of the support plate. A through hole for inserting cryopreservation tubes is provided in the support plate. Limiting blocks are provided at the four corners of the insulated box, and the four limiting blocks and the inner wall of the insulated box form four slots for inserting the heat exchange plate. The heat exchange plate is inserted into the slot and provides a cold source to the inside of the insulated box. A temperature sensor is provided at the bottom of the inside of the insulated box, and the controller assembly is installed at the front end of the insulated box.

[0007] Preferably, the heat exchange plate includes a shell, a phase change material, a heat exchange plate, fins, a liquid injection port, and a sealing cap. The shell is filled with a phase change material. A heat exchange plate is provided on one side of the shell, and fins are provided on both the inner and outer sides of the heat exchange plate. A liquid injection port is provided on the top of the shell, and the liquid injection port is sealed by the sealing cap.

[0008] Preferably, the controller assembly includes a controller body, a display screen, a battery compartment, and connecting cables. The display screen is embedded in the front end of the controller body, and a battery compartment for installing a power supply battery is provided at the bottom front side of the controller body. The controller body is electrically connected to a temperature sensor via connecting cables, and the controller body is fixed to the outer wall of the insulation box.

[0009] Preferably, the top right side of the insulated box is rotatably connected to the top cover via a pivot, and the left side of the top cover is locked to the insulated box via a locking buckle. The insulated box and the top cover are sealed together by a sealing ring.

[0010] Preferably, the height of the heat exchange plate is lower than the height of the support edge.

[0011] Preferably, the heat exchange plate and fins are made of copper, and the heat exchange plate is sealed to the shell.

[0012] Preferably, the phase change material is an organic PCM.

[0013] (III) Beneficial Effects This invention provides a lightweight plant tissue sample cold storage box. It has the following advantages:

[0014] 1. A heat exchange plate based on phase change material (PCM) is used as the cold source. During the phase change process, it can absorb a large amount of heat, so that the temperature inside the box is stable near the phase change point for a long time. This avoids the problem of continuous temperature rise in ordinary insulated boxes, and provides a constant low temperature preservation environment for samples. The heat exchange area is increased by copper heat exchange plate and fins, which ensures uniform distribution of cold energy inside the box. 2. The integrated temperature sensor and controller components can display the temperature inside the box on the screen in real time, allowing users to accurately monitor the pre-cooling progress and temperature status during storage, greatly improving the reliability and safety of sample storage. 3. The core components such as heat exchange plates and support plates are all detachable modular designs, which not only reduces the overall weight and makes them easy to carry, but also makes pre-cooling, cleaning and maintenance more flexible and convenient, perfectly meeting the needs of field operations. 4. The entire system does not require external power during the storage phase, and works solely based on the physical properties of the phase change material. It is energy-saving and environmentally friendly. The phase change material can be repeatedly frozen and thawed, and its long-term use cost is far lower than that of consumables such as dry ice. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the internal structure of the insulated box of this utility model; Figure 2 This is a top view of the internal structure of the insulated box of this utility model; Figure 3 This is a top view of the limiting block structure in this utility model; Figure 4 This is a schematic diagram of the internal structure of the heat exchange plate in this utility model; Figure 5 This is a schematic diagram of the controller component structure in this utility model.

[0016] In the diagram: Insulation box-1, top cover-2, support edge-3, support plate-4, cryopreservation tube-5, limit block-6, heat exchange plate-7, temperature sensor-8, controller assembly-9; Shell-71, phase change material-72, heat exchange plate-73, fins-74, liquid injection port-75, sealing cap-76; Controller body - 91, display screen - 92, battery compartment - 93, connecting cable - 94. Detailed Implementation

[0017] The following is in conjunction with the appendix Figure 1 - Appendix Figure 5 This application will be described in further detail below.

[0018] A lightweight plant tissue sample cold storage box, as described above Figure 1 The device includes an insulation box 1, a limiting block 6, a heat exchange plate 7, a temperature sensor 8, and a controller assembly 9. The top of the insulation box 1 is sealed by a top cover 2. A support edge 3 is provided in the middle of the inner wall of the insulation box 1. The support edge 3 is used to support the bottom edge of the support plate 4. A through hole for inserting a cryopreservation tube 5 is opened in the support plate 4. Limiting blocks 6 are provided at the four corners of the interior of the insulation box 1. The four limiting blocks 6 and the inner wall of the insulation box 1 form four slots for inserting the heat exchange plate 7. The heat exchange plate 7 is inserted into the slot 7. The heat exchange plate 7 is used to provide a cold source to the interior of the insulation box 1. A temperature sensor 8 is provided at the bottom of the interior of the insulation box 1. The controller assembly 9 is installed at the front end of the insulation box 1. The top right side of the heat preservation box 1 is rotatably connected to the top cover 2 via a pivot, and the left side of the top cover 2 is locked to the heat preservation box 1 via a locking buckle. The heat preservation box 1 and the top cover 2 are sealed together by a sealing ring. The height of the heat exchange plate 7 is lower than the height of the support edge 3 to avoid the heat exchange plate 7 affecting the placement of the support plate 4. The support plate 4 is attached to the inner wall of the insulation box 1 to limit the movement of the support plate 4. The heat exchange plate 73 and fins 74 are made of copper. The heat exchange plate 73 is sealed to the shell 71. The use of copper ensures heat exchange efficiency. The fins 74 on the inner and outer sides of the heat exchange plate 73 further increase the heat exchange efficiency. Phase change material 72 uses organic PCM. Organic PCM is chemically stable, not easily decomposed, has a long cycle life, and does not exhibit supercooling or phase separation, which is its biggest advantage over hydrated salts. It is non-corrosive, has low requirements for encapsulation materials, and has a wide phase change temperature range, which can be customized through mixing. Organic PCMs with different phase transition points are selected based on different plant tissue samples. For example: 1. Ice (water), with a phase transition point of 0℃, is the most natural and cheapest PCM. 2. Fatty acids (and their derivatives), such as lauric acid (phase change point of about 44℃, which needs to be mixed with other acids to lower the melting point), decanoic acid, palmitic acid, etc., can be used to precisely formulate phase change materials with a temperature of 0-4℃ by mixing different proportions of fatty acids. 3. Paraffinic hydrocarbons, n-tetradecane (phase transition point about 5.5℃) and n-pentadecane (phase transition point about 10℃, must be used in combination). Paraffin has strong chemical inertness, stable properties, and is non-corrosive. 4. Polyethylene glycol (PEG): By selecting different molecular weights, different phase transition temperatures can be obtained.

[0019] Reference Figure 4 The heat exchange plate 7 includes a shell 71, a phase change material 72, a heat exchange plate 73, fins 74, a liquid injection port 75, and a sealing cap 76. The shell 71 is filled with phase change material 72. A heat exchange plate 73 is provided on one side of the shell 71, and fins 74 are provided on both the inner and outer sides of the heat exchange plate 73. A liquid injection port 75 is provided on the top of the shell 71, and the liquid injection port 75 is sealed by the sealing cap 76.

[0020] Reference Figure 5 The controller assembly 9 includes a controller body 91, a display screen 92, a battery compartment 93, and a connecting cable 94. The display screen 92 is embedded in the front end of the controller body 91. The battery compartment 93 for installing the power supply battery is provided at the bottom front side of the controller body 91. The controller body 91 is electrically connected to the temperature sensor 8 through the connecting cable 94. The controller body 91 is fixed to the outer wall of the insulation box 1. The controller body 91 integrates a microprocessor. The controller body 91 receives analog or digital electrical signals collected by the temperature sensor 8 through the connection line 94. The internal microprocessor converts and processes the signals, calibrates them into actual temperature values, and drives the display screen 92 to display the current temperature in digital form in real time. The entire process is powered by the battery in the battery compartment 93.

[0021] The core of this cold storage box lies in utilizing the property of the phase change material 72 in the heat exchange plate 7 to absorb a large amount of heat when it undergoes a phase change, thereby maintaining a low-temperature environment inside the box. Its specific working process is as follows: First, pre-cooling preparation is carried out. Before use, the heat exchange plate 7 is taken out from the slot formed by the limiting block 6 on the side wall of the heat preservation box 1. After pouring the selected organic phase change material (PCM) with the selected phase change temperature through the liquid injection port 75 on its top, it is sealed with the sealing cap 76 and placed in the freezing equipment for full pre-cooling, so that the phase change material 72 solidifies and stores a large amount of cold energy. Next, start pre-cooling and temperature monitoring. Quickly insert the pre-cooled heat exchange plate 7 back into the slots at the four corners of the insulation box 1 and close the top cover 2 tightly. At this time, the heat exchange plate 7 starts to work as a cold source. The cold energy stored inside is efficiently released to the air inside the box through the heat exchange plate 73 and fins 74 made of highly thermally conductive copper. During this pre-cooling process, the temperature sensor 8 installed at the bottom of the box monitors the temperature change in real time and transmits the data to the controller assembly 9 through the connection line 94. The user can intuitively observe the temperature drop inside the box through the display screen 92. After the temperature stabilizes and drops to the preset required storage temperature (such as 4℃ or 0℃), the next step can be performed. Next, the sample is placed inside. Once the target temperature is reached inside the box, the top cover 2 is opened, and the cryopreservation tubes 5 containing the plant tissue samples are inserted one by one into the through holes of the support plate 4. Then, the support plate 4 is placed stably on the support edge 3. Since the height of the heat exchange plate 7 is lower than the height of the support edge 3, it ensures that the support plate 4 is placed stably and does not affect the cold air circulation. Then, the top cover 2 is quickly closed and locked. The sealing ring between the heat preservation box 1 and the top cover 2 effectively maintains the airtightness and low temperature environment inside the box. Finally, the continuous cooling and monitoring stage begins. During sample preservation, the phase change material 72 continuously absorbs heat from the outside to maintain a stable temperature inside the box. The temperature sensor 8 and controller assembly 9 work continuously to provide users with real-time temperature display, ensuring that the sample is always kept in a safe low-temperature state.

[0022] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A lightweight plant tissue sample cold storage box, comprising an insulated box (1), the top of the insulated box (1) being sealed by a top cover (2), a support edge (3) being provided in the middle of the inner wall of the insulated box (1), the support edge (3) being used to support the bottom edge of a support plate (4), and a through hole for inserting a cryopreservation tube (5) being provided in the support plate (4); Its features are: It also includes a limiting block (6), a heat exchange plate (7), a temperature sensor (8), and a controller assembly (9). The four corners of the interior of the heat insulation box (1) are provided with limiting blocks (6), and the four limiting blocks (6) and the inner wall of the heat insulation box (1) form four slots for inserting the heat exchange plate (7). The heat exchange plate (7) is inserted into the slot. The heat exchange plate (7) is used to provide a cold source to the interior of the heat insulation box (1). The temperature sensor (8) is provided at the bottom of the interior of the heat insulation box (1). The controller assembly (9) is installed at the front end of the heat insulation box (1).

2. The portable plant tissue sample cold storage box according to claim 1, characterized in that: The heat exchange plate (7) includes a shell (71), a phase change material (72), a heat exchange plate (73), fins (74), a liquid injection port (75), and a sealing cap (76). The shell (71) is filled with phase change material (72). A heat exchange plate (73) is provided on one side of the shell (71), and fins (74) are provided on both the inner and outer sides of the heat exchange plate (73). A liquid injection port (75) is provided on the top of the shell (71), and the liquid injection port (75) is sealed by the sealing cap (76).

3. The portable plant tissue sample cold storage box according to claim 1, characterized in that: The controller assembly (9) includes a controller body (91), a display screen (92), a battery compartment (93), and a connecting cable (94). The display screen (92) is embedded in the front end of the controller body (91). A battery compartment (93) for installing a power supply battery is provided at the bottom front side of the controller body (91). The controller body (91) is electrically connected to the temperature sensor (8) through the connecting cable (94). The controller body (91) is fixed to the outer wall of the heat preservation box (1).

4. The portable plant tissue sample cold storage box according to claim 1, characterized in that: The top right side of the heat preservation box (1) is rotatably connected to the top cover (2) via a pivot, and the left side of the top cover (2) is locked to the heat preservation box (1) via a locking buckle. The heat preservation box (1) and the top cover (2) are sealed together by a sealing ring.

5. A portable plant tissue sample cold storage box according to claim 1, characterized in that: The height of the heat exchange plate (7) is lower than the height of the support edge (3).

6. A lightweight plant tissue sample cold storage box according to claim 2, characterized in that: The heat exchange plate (73) and fins (74) are made of copper, and the heat exchange plate (73) is sealed to the shell (71).

7. A portable plant tissue sample cold storage box according to claim 2, characterized in that: The phase change material (72) is an organic PCM.

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