Phage incubator tank for easy post-experiment autoclaving

Abstract

The utility model aims at giving convenient experiment high temperature disinfection's bacteriophage cultivation jar, it can prevent second trachea together with bubble stone to shake. Convenient experiment high temperature disinfection's bacteriophage cultivation jar, including cultivation jar body, the cultivation jar body is equipped with culture solution and host bacteria in, the bottle mouth of cultivation jar body is equipped with the bottle lid, the rear part of bottle lid is fixed with the fixed pipe of vertical through itself, the upper end of fixed pipe is used to install first trachea to connect air pump, the lower part of fixed pipe is equipped with second trachea, the first end of second trachea is equipped on the lower end of fixed pipe, the middle part is equipped with check valve in series, the end is equipped with bubble stone and is soaked into culture solution, the front part of bottle lid is equipped with the outlet, the front wall of bottle lid is equipped with the bacteria-throwing convex mouth and is connected with the outlet, the front part and the rear part of bottle lid are connected through the hinge shaft, the rear part of bottle lid is fixedly bonded on the bottle mouth rear part, the bacteria-throwing convex mouth is stainless steel material, and the front part of bottle lid is pressed on the bottle mouth front part.

Description

Technical Field

[0001] This utility model relates to the field of phage culture experiments, and in particular to phage culture containers. Background Technology

[0002] Existing phage culture equipment such as Figure 1 As shown, the system includes a culture tank body 1, which contains a culture medium 2 and host bacteria. The outer wall of the bottle mouth 3 of the culture tank body 1 is threaded, and an internally threaded cap 4 is threaded onto the bottle mouth 3. A vertically penetrating fixing tube 30 is fixed to the rear of the internally threaded cap 4. A first air pipe 11 is located above the fixing tube 30, with an air pump 22 connected to its first end, and its end fitted onto the upper end 10 of the fixing tube. A second air pipe 7 is located below the fixing tube 30, with its first end fitted onto the lower end 6 of the fixing tube, a check valve 8 connected in series in the middle, and an air stone 9 attached to its end and immersed in the culture medium 2. An air outlet is opened at the front of the internally threaded cap 4, and a bacteria inoculation nozzle 5 is installed on the top wall of the internally threaded cap 4, connecting to the air outlet. The top of the bacteria inoculation nozzle 5 is sealed with a breathable sealing cloth (not shown in the figure). The experimental procedure is as follows: Air is pumped by air pump 22 to the first air pipe 11, then passes through the fixed pipe 30, the second air pipe 7, and the check valve 8 to reach the air stone 9. At the air stone 9, air bubbles are released into the culture medium 2. The oxygen in the bubbles is consumed by the host bacteria during respiration and replication. Excess gas in the bubbles rises to the top of the culture medium 2 and is then discharged through the air outlet and the breathable sealing cloth. After a certain period, when the host bacteria have replicated sufficiently, the operator opens the breathable sealing cloth and inserts bacteriophages into the culture tank body 1 through the inoculation nozzle 5. The breathable sealing cloth is then resealed, and the bacteriophages are cultured using the host bacteria. After each experiment, the culture tank body 1 needs to be sterilized in a steam oven at high temperature before use. The drawback of the existing technology is that, for thorough sterilization, the operator needs to unscrew the internally threaded cap 4 before high-temperature sterilization to allow hot steam to enter the culture tank body 1 for sterilization. However, unscrewing the cap causes the second air pipe 7 and the air stone 9 to shake, which can easily damage the glass culture tank body 1. Utility Model Content

[0003] The present invention aims to provide a phage culture vessel that is convenient for high-temperature sterilization after experiments, and can prevent the second trachea and air stone from shaking.

[0004] A phage culture vessel designed for convenient high-temperature sterilization after experiments includes a vessel body containing culture medium and host bacteria. The vessel body has a cap at the mouth, with a vertically extending tube fixed to the rear. The upper end of the tube is used to install a first air tube for connecting to an air pump, and a second air tube is located below it. The first end of the second air tube is fitted onto the lower end of the fixed tube, a check valve is connected in series in the middle, and an air stone is installed at the end, immersed in the culture medium. An air vent is located at the front of the cap, and a bacterial inoculation nozzle is installed on the top wall of the front of the cap, connecting to the air vent. The front and rear of the cap are connected by a hinge. The rear of the cap is fixedly attached to the rear edge of the vessel mouth, and the bacterial inoculation nozzle, made of stainless steel, presses the front of the cap against the front edge of the vessel mouth.

[0005] Furthermore, the weight of the inoculation nozzle is 200g.

[0006] Furthermore, a vertical rod is installed at the front of the bottle cap at the upper end of the fixing tube, and a support block is installed on the top wall of the vertical rod; when the bottle cap is operated, the front of the bottle cap, together with the inoculation nozzle, rotates 90° backward around the hinge axis, and the support block supports the rear side wall of the inoculation nozzle.

[0007] Furthermore, the fixing tube is located behind the vertical rod.

[0008] Furthermore, the support block and the second air tube are both made of heat-resistant silicone; the fixing tube is made of stainless steel.

[0009] The beneficial effects are as follows: After completing the experiment, the operator removes the first air tube, then lifts the front of the bottle cap around the hinge to open the front of the bottle mouth, and then sends the culture tank into the steam oven for high-temperature sterilization. Hot steam enters the culture tank through the front of the bottle mouth for sterilization. Because the rear of the bottle cap is not operated, the second air tube and the air stone will not move, and will not shake or damage the culture tank. Attached Figure Description

[0010] Figure 1 This is a left view of an existing phage culture device; Figure 2 This is a left view of the bottle mouth (3) of this incubator. In the figure, the front part (41) of the bottle cap is in a horizontal position to close the front part of the bottle mouth (3). Figure 3 This is a top view of the incubator. In the figure, the first air tube at the upper end (10) of the fixed tube has been removed, and the front part (41) of the bottle cap is in a horizontal position to close the front part of the bottle mouth. Figure 4 This is a left view of the bottle mouth (3) of this incubator. In the figure, the first air tube at the upper end (10) of the fixed tube has been removed, and the front part (41) of the bottle cap is in a vertical position with the front part of the bottle mouth (3) open. In the diagram: 1. Culture tank body; 2. Culture medium; 3. Bottle mouth; 4. Internally threaded bottle cap; 5. Inoculation nozzle; 6. Lower end of the fixing tube; 7. Second air pipe; 8. Check valve; 9. Air stone; 10. Upper end of the fixing tube; 11. First air pipe; 22. Air pump; 30. Fixing tube; 41. Front part of the bottle cap; 42. Vertical rod; 45. Hinge; 47. Rear part of the bottle cap; 50. Support block; 60. Rear side wall; 61. Air outlet. Detailed Implementation

[0011] The present invention will be further described in detail below with reference to specific embodiments.

[0012] The only difference between this culture vessel and existing culture vessels is the cap. The cap of this culture vessel is as follows: Figure 3 As shown, the front part 41 and the rear part 47 of the bottle cap are connected to each other by left and right transverse hinges 45. Figure 2 and Figure 3 As shown, a vertically penetrating fixing tube 30 is installed behind the top wall of the rear part 47 of the bottle cap. The upper end 10 of the fixing tube is sleeved by the end of the first air tube 11, which is connected to the air pump 22 (see...). Figure 1 A second air pipe 7 is provided below the fixed pipe 30. The first end of the second air pipe 7 is fitted onto the lower end 6 of the fixed pipe, a check valve 8 is installed in series in the middle, and an air stone 9 is installed at the end (see...). Figure 1 A vertical rod 42 is installed on the top wall of the rear part 47 of the bottle cap in front of the upper end 10 of the fixing tube. A heat-resistant silicone support block 50 is installed on the top of the vertical rod 42. A vertically penetrating vent 61 is opened on the front part 41 of the bottle cap, located directly in front of the vertical rod 42. A bacteria-feeding nozzle 5 is installed on the top wall of the front part 41 of the bottle cap above the vent 61. The bacteria-feeding nozzle 5 is covered with a breathable sealing cloth. Figure 2 As shown, the rear part 47 of the bottle cap is fixedly attached to the rear edge of the bottle mouth 3 of the culture tank body 1, and the air stone 9 is like... Figure 1 The bacteria are immersed in the culture medium 2 inside the culture tank body 1 as shown to provide oxygen to the host bacteria in the culture medium 2. The inoculation nozzle 5 is made of stainless steel and weighs 200g. The front part 41 of the bottle cap is pressed against the front edge of the bottle mouth 3.

[0013] The experimental process and principle are the same as existing technologies and will not be repeated here. After the experiment, the culture tank body 1 needs to be sterilized. The experimenter operates the front part 41 of the bottle cap and rotates it along with the inoculation nozzle 5 around the hinge axis 45 by 90° to open the front part of the bottle mouth 3. The rear side wall 60 of the inoculation nozzle 5 will then be supported by the support block 50. The experimenter removes the first air tube 11 from the upper end 10 of the fixing tube and places the culture tank body 1 into a steam oven (not shown in the figure) for high-temperature sterilization. Hot steam enters the culture tank body 1 through the front part of the bottle mouth 3 for sterilization. The second air tube 7 is made of heat-resistant silicone, and the fixing tube 30 is made of stainless steel, so they are not afraid of high temperatures.

[0014] In a less preferred embodiment, the heat-resistant silicone support block 50 can be replaced with a heat-resistant silicone suction cup, which rotates 90 degrees around the hinge axis 45 at the front part 41 of the bottle cap under the influence of the operation. Figure 4 In the vertical position, the suction cup adheres to the rear side wall 60 of the inoculation nozzle 5 to prevent the front part 41 of the bottle cap from accidentally rotating forward and resetting.

[0015] The above description is merely an embodiment of this utility model and does not limit the scope of patent protection. Any non-substantial changes or substitutions made by those skilled in the art based on this utility model will still fall within the scope of patent protection.

Claims

1. A phage culture vessel that is convenient for high-temperature sterilization after experiments, comprising a culture vessel body (1), wherein the culture vessel body (1) contains a culture medium (2) and host bacteria; the bottle mouth (3) of the culture vessel body (1) is fitted with a bottle cap, and a vertically penetrating fixed tube (30) is fixedly installed at the rear part (47) of the bottle cap, the upper end (10) of the fixed tube is used to install a first air tube (11) to connect to an air pump (22), a second air tube (7) is provided below the fixed tube (30), the first end of the second air tube (7) is fitted onto the lower end (6) of the fixed tube, a check valve (8) is installed in series in the middle, and an air stone (9) is installed at the end and immersed in the culture medium (2); an air outlet is opened at the front part (41) of the bottle cap, and a bacterial feeding nozzle (5) is installed on the top wall of the front part (41) of the bottle cap to connect to the air outlet, characterized in that: The front part (41) and the rear part (47) of the bottle cap are connected by a hinge (45); the rear part (47) of the bottle cap is fixedly glued to the rear part of the upper edge of the bottle mouth (3), and the inoculation nozzle (5) is made of stainless steel, pressing the front part (41) of the bottle cap onto the front part of the upper edge of the bottle mouth (3).

2. The phage culture vessel as described in claim 1, characterized in that: The weight of the inoculation nozzle is 200g.

3. The phage culture vessel as described in claim 1, characterized in that: The front part (41) of the bottle cap is equipped with a vertical rod (42) in front of the upper end (10) of the fixing tube, and a support block (50) is installed on the top wall of the vertical rod (42); when the front part (41) of the bottle cap is operated, it rotates 90° backward along with the inoculation nozzle (5) around the hinge axis (45), and the support block (50) supports the rear side wall (60) of the inoculation nozzle (5).

4. The phage culture vessel as described in claim 3, characterized in that: The fixed tube (30) is located behind the vertical rod (42).

5. The phage culture vessel as described in claim 1, characterized in that: The support block (50) and the second air tube (7) are both made of heat-resistant silicone; the fixing tube is made of stainless steel.

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