Temperature-sensitive microorganism precision culture and rapid detection integrated device

Abstract

The utility model discloses a temperature sensitive type microorganism precision culture and rapid detection integrated device belongs to laboratory microorganism culture technical field, including device main part, culture module, temperature control module, environmental regulation module, monitoring module and control terminal, the present application in the culture process, ensure that temperature deviation is in the small range rapid convergence, avoid the temperature overshoot and lag of traditional incubator because of the door of frequently switching, environmental fluctuation or heating inertia, thereby provide accurate stable growth environment for temperature sensitive strain, on the other hand, the synergic control of dissolved oxygen, humidity and pH avoids the random error and pollution risk brought by manual adjustment, makes the culture environment keep homogeneity for a long time, high definition camera cooperation fluorescence detection module realizes no open cover observation, dynamic data acquisition and quantitative detection, need not shift petri dish to can directly complete monitoring and analysis, significantly reduce the operation step and cross contamination possibility.

Description

Technical Field

[0001] This utility model relates to the field of laboratory microbial culture technology, specifically to an integrated device for precise culture and rapid detection of temperature-sensitive microorganisms. Background Technology

[0002] Temperature-sensitive microorganisms are a class of strains that are highly sensitive to changes in culture temperature. Their growth, metabolism, and replication capabilities often exhibit significant variations within a very narrow temperature range. For example, some mutants can grow normally at low temperatures, but will rapidly become inactive, misfold their proteins, or cease reproduction when approaching the upper limit of the allowable temperature. Similarly, some industrial strains will exhibit stress responses outside their optimal temperature range, leading to a decline in product quality. Therefore, the stability and precision of culture temperature are extremely important in microbial culture, strain preservation, large-scale domestication, and functional studies.

[0003] Several significant technical bottlenecks currently exist in the laboratory cultivation of temperature-sensitive microorganisms. First, traditional microbial incubators suffer from insufficient temperature control precision, with fluctuations typically exceeding ±0.5℃. This can easily lead to growth stagnation or even death for extremely temperature-sensitive strains. Furthermore, manually opening the incubator to observe colony growth introduces additional temperature interference.

[0004] Secondly, the existing culture and detection processes are separate. After the strain has grown in the incubator, it needs to be transferred to a separate detection device for analysis. This transfer process is not only time-consuming, but also carries a high risk of sample contamination and damage to strain viability due to temperature changes and improper operation, which is not conducive to rapid clinical diagnosis.

[0005] Furthermore, under low-temperature culture conditions, the range of microorganisms' adaptation to environmental parameters such as pH and dissolved oxygen becomes narrower, and existing culture devices generally lack the ability to coordinate and precisely regulate these key environmental factors, which seriously affects the success rate of culture and the accuracy of subsequent test results.

[0006] Although there are independent low-temperature incubators and microbial detection equipment on the market, there is a lack of a device designed specifically for temperature-sensitive microorganisms that integrates culture, monitoring, detection, and coordinated control of multiple environmental parameters, which cannot meet the needs of modern laboratories for accurate, efficient, and automated microbial analysis. Utility Model Content

[0007] The purpose of this invention is to provide an integrated device for the precise culture and rapid detection of temperature-sensitive microorganisms, enabling precise temperature-controlled culture, coordinated regulation of multiple environmental parameters, real-time monitoring of the growth process, and rapid in-situ detection after culture. This effectively reduces the risk of strain death and contamination during transfer, significantly shortens the detection cycle, and improves the efficiency and accuracy of laboratory microbial detection.

[0008] To solve the above-mentioned technical problems, the present invention adopts the following technical solution: An integrated device for precise culture and rapid detection of temperature-sensitive microorganisms includes: The main body of the device is a sealed box structure with an insulation layer, an observation window and a sealing cover; A culture module, located inside the main body of the device, is used to hold the culture dish; Temperature control module, used to precisely control the temperature inside the main body of the device; An environmental control module is used to regulate and stabilize the dissolved oxygen concentration, humidity, and pH value inside the main body of the device. The monitoring module is used to collect colony images and environmental parameter data in real time; The detection module is used for fluorescence detection of cultured microorganisms; The control terminal is used to set parameters, display data, and control the operation of various modules. The culture module, temperature control module, environmental regulation module, monitoring module, and detection module are integrated within the main body of the device and are uniformly controlled by the control terminal.

[0009] A further technical solution is that the temperature control module includes a cooler, a heating element, a temperature sensor, and an intelligent control unit; the intelligent control unit is used to control the operation of the cooler and the heating element.

[0010] A further technical solution is that the environmental control module includes: The dissolved oxygen control unit includes a miniature air pump, an oxygen concentration sensor, and a gas flow controller; A humidity control unit, which includes an ultrasonic humidifier and a humidity sensor; The pH-assisted stabilization unit includes a pH buffer tray and a pH monitoring sensor located below the culture module.

[0011] A further technical solution is that the monitoring module includes a high-definition industrial camera and an environmental data display unit; the environmental data display unit is used to integrate and display temperature, humidity, oxygen concentration and pH value data.

[0012] A further technical solution is that the detection module includes: The fluorescence detection unit has an adjustable wavelength LED excitation source and a photomultiplier tube fluorescence receiver.

[0013] A further technical solution is that the culture module includes a culture rack; the culture rack is provided with a fixing groove for fixing the culture dish; and the bottom of the fixing groove is provided with a gas circulation hole.

[0014] A further technical solution is that the control terminal is a touch screen display; the touch screen display has a built-in data storage module; and the touch screen display is equipped with a USB interface for exporting data.

[0015] Compared with the prior art, the beneficial effects of this utility model are: During the cultivation process, this application ensures that temperature deviations converge rapidly within a very small range, avoiding temperature overshoot and lag caused by frequent door opening and closing, environmental fluctuations, or heating inertia in traditional incubators. This provides a precise and stable growth environment for temperature-sensitive strains. On the other hand, the coordinated regulation of dissolved oxygen, humidity, and pH avoids random errors and contamination risks caused by manual adjustments, ensuring the long-term uniformity of the cultivation environment. A high-definition camera combined with a fluorescence detection module enables observation without opening the lid, dynamic data acquisition, and quantitative detection. Monitoring and analysis can be completed directly without transferring culture dishes, significantly reducing operational steps and the possibility of cross-contamination. Attached Figure Description

[0016] To make the objectives, technical solutions, and beneficial effects of this utility model clearer, the following drawings are provided for illustration: Figure 1 This is a three-dimensional drawing of the present invention.

[0017] Figure 2 This is a three-dimensional view of a partial structure of the main body of the device of this utility model.

[0018] Figure 3 This is a three-dimensional structural diagram of the device body of this utility model.

[0019] Figure 4 This is a three-dimensional view of a partial structure within the main body of the device of this utility model.

[0020] Figure 5 This is a three-dimensional view of the structure below the cultivation module of this utility model.

[0021] Icons: 1. Baffle; 2. Transparent observation window; 3. Sealed cover; 4. Anti-slip pad; 5. Culture rack; 6. Fixing tank; 7. Temperature control module; 8. Refrigerator; 9. Heating element; 10. Temperature sensor; 11. Intelligent control unit; 2. Environmental control module; 3. Miniature air pump; 4. Oxygen concentration sensor; 5. Gas flow controller; 6. Ultrasonic humidifier; 74. Humidity sensor; 75. pH buffer tray; 76. pH monitoring sensor; 77. Monitoring module; 8. High-definition industrial camera; 91. Environmental data display unit; 82. Detection module; 9. LED excitation light source; 92. Photomultiplier tube fluorescence receiver; 93. Horizontal electric cylinder; 94. Vertical electric cylinder; 95. Detection board; 10. Control terminal; 10. USB interface. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model.

[0023] Example: like Figures 1-5 As shown, this utility model provides an integrated device for precise culture and rapid detection of temperature-sensitive microorganisms, including a main body, a culture module, a temperature control module 6, an environmental control module 7, a monitoring module 8, and a control terminal 10. The main body is a sealed box structure with a double insulation layer: an inner layer of polyurethane insulation cotton and an outer layer of 304 stainless steel insulation. The main body includes a hinged baffle 1. Both sides of the main body have double-layered anti-fog high borosilicate glass transparent observation windows 2. The main body also includes a top-sealed cover 3, which uses a food-grade silicone sealing ring with an IP67 sealing rating. The bottom of the main body is equipped with four shock-absorbing and anti-slip feet 4.

[0024] The culture module includes a culture rack 5 located on the bottom wall of the main body of the device; the culture rack 5 includes a culture plate and four culture feet fixed below the culture plate; the culture plate is provided with multiple fixing grooves 51 for fixing the culture dishes; the bottom of the fixing grooves 51 is provided with gas circulation holes.

[0025] The temperature control module 6 includes a cooler 61, a heating element 62, a temperature sensor 63, and an intelligent control unit 64. The intelligent control unit 64 controls the operation of the cooler 61 and the heating element 62. The cooler 61 and the heating element 62 are symmetrically arranged on the inner wall of the chamber. The temperature sensor 63 is fixed to the surface of the culture plate and is used to collect multi-point temperatures in real time. The intelligent control unit 64 uses an STM32 microcontroller and controls the power of the cooler 61 and the heating element 62 through a PID algorithm.

[0026] The environmental control module 7 includes a dissolved oxygen control unit, a humidity control unit, and a pH-assisted stabilization unit. The dissolved oxygen control unit includes a miniature air pump 71, an oxygen concentration sensor 72, and a gas flow controller 73, all located on the bottom wall of the device body. The gas flow controller 73 is installed at the outlet of the miniature air pump 71. The humidity control unit includes an ultrasonic humidifier 74 and a humidity sensor 75, all located on the bottom wall of the device body. The pH-assisted stabilization unit includes a pH buffer tray 76 located below the culture plate and a pH monitoring sensor 77. The pH monitoring sensor 77 is located inside the pH buffer tray 76.

[0027] The monitoring module 8 includes a high-definition industrial camera 81 mounted on the top wall of the device body and an environmental data display unit 82 mounted on the outer wall of the device body; the environmental data display unit 82 is used to integrate and display temperature, humidity, oxygen concentration and pH value data; the environmental data display unit 82 is a display screen.

[0028] The detection module 9 includes a fluorescence detection unit, which has an adjustable wavelength LED excitation light source 91 and a photomultiplier tube fluorescence receiver 92; a horizontal electric cylinder 93 is provided on the inner side wall of the device body; a detection frame is provided at the telescopic end of the horizontal electric cylinder 93; a vertical electric cylinder 94 is provided on the bottom side of the detection frame; the LED excitation light source 91 and the photomultiplier tube fluorescence receiver 92 are fixed to the telescopic end of the vertical electric cylinder 94 through a detection plate 95.

[0029] The control terminal 10 is a touch screen; the touch screen has a built-in data storage module; and the touch screen is equipped with a USB interface 101 for exporting data.

[0030] The principles and beneficial effects of the above technical solution: Through multi-channel communication between the control terminal 10 and multiple actuators within the device, centralized intelligent control of processes such as cultivation, temperature control, gas regulation, and fluorescence detection is achieved. Specifically, the control terminal 10 serves as the core operating interface, sending user-defined parameters such as cultivation temperature, humidity, oxygen concentration, and detection cycle to the intelligent control unit 64. The intelligent control unit 64, based on an STM32 processor, acquires data in real time from the temperature sensor 63, humidity sensor 75, gas concentration sensor, and pH sensor. It then uses a PID algorithm to perform closed-loop regulation of actuators such as the cooler 61, heating element 62, micro air pump 71, flow controller, humidifier, horizontal electric cylinder 93, vertical electric cylinder 94, and excitation light source 91. When the data from the monitoring module 8 and detection module 9 is returned and integrated by the control terminal 10, the control logic determines whether to perform operations such as cooling, heating, humidification, gas supply, or fluorescence detection based on the deviation, thereby achieving adaptive microenvironment control. Through this automated closed-loop control mechanism, the device can dynamically maintain the stability of various environmental parameters in the incubator without relying on human intervention, and realize the automatic switching between the culture stage, monitoring stage and detection stage.

[0031] During the cultivation process, the PID temperature control mechanism ensures that the temperature deviation converges rapidly within a very small range, avoiding the temperature overshoot and lag caused by frequent door opening and closing, environmental fluctuations, or heating inertia in traditional incubators. This provides a precise and stable growth environment for temperature-sensitive strains. On the other hand, the coordinated regulation of dissolved oxygen, humidity, and pH avoids random errors and contamination risks caused by manual adjustments, ensuring that the cultivation environment remains uniform over a long period. A high-definition camera, in conjunction with the fluorescence detection module 9, enables observation without opening the lid, dynamic data acquisition, and quantitative detection. Monitoring and analysis can be completed directly without transferring culture dishes, significantly reducing operational steps and the possibility of cross-contamination.

[0032] Overall, this device integrates culture, environmental control, imaging monitoring, and fluorescence detection, overcoming the limitations of existing technologies such as "multiple devices operating separately, manual sample transport leading to large temperature fluctuations, cumbersome operation, and high risk of contamination." It enables precise culture and rapid detection of temperature-sensitive microorganisms, improving experimental stability, repeatability, and automation.

[0033] Although the present invention has been described herein with reference to several illustrative embodiments, it should be understood that many other modifications and implementations can be devised by those skilled in the art, which will fall within the scope and spirit of the principles disclosed herein. More specifically, various variations and modifications can be made to the components and / or layout of the subject matter combination within the scope of the disclosure, drawings, and claims. Besides variations and modifications to the components and / or layout, other uses will be apparent to those skilled in the art.

Claims

1. An integrated device for precise culture and rapid detection of temperature-sensitive microorganisms, characterized in that, include: The main body of the device is a sealed box structure with an insulation layer, an observation window and a sealing cover; A culture module, located inside the main body of the device, is used to hold the culture dish; Temperature control module, used to precisely control the temperature inside the main body of the device; An environmental control module is used to regulate and stabilize the dissolved oxygen concentration, humidity, and pH value inside the main body of the device. The monitoring module is used to collect colony images and environmental parameter data in real time; The detection module is used for fluorescence detection of cultured microorganisms; The control terminal is used to set parameters, display data, and control the operation of various modules. The culture module, temperature control module, environmental regulation module, monitoring module, and detection module are integrated within the main body of the device and are uniformly controlled by the control terminal.

2. The integrated device for precise culture and rapid detection of temperature-sensitive microorganisms according to claim 1, characterized in that: The temperature control module includes a cooler, a heating element, a temperature sensor, and an intelligent control unit; the intelligent control unit is used to control the operation of the cooler and the heating element.

3. The integrated device for precise culture and rapid detection of temperature-sensitive microorganisms according to claim 1, characterized in that: The environmental control module includes: The dissolved oxygen control unit includes a miniature air pump, an oxygen concentration sensor, and a gas flow controller; A humidity control unit, which includes an ultrasonic humidifier and a humidity sensor; The pH-assisted stabilization unit includes a pH buffer tray and a pH monitoring sensor located below the culture module.

4. The integrated device for precise culture and rapid detection of temperature-sensitive microorganisms according to claim 1, characterized in that: The monitoring module includes a high-definition industrial camera and an environmental data display unit; the environmental data display unit is used to integrate and display temperature, humidity, oxygen concentration and pH value data.

5. The integrated device for precise culture and rapid detection of temperature-sensitive microorganisms according to claim 1, characterized in that: The detection module includes: The fluorescence detection unit has an adjustable wavelength LED excitation source and a photomultiplier tube fluorescence receiver.

6. The integrated device for precise culture and rapid detection of temperature-sensitive microorganisms according to claim 1, characterized in that: The culture module includes a culture rack; the culture rack is provided with a fixing groove for fixing the culture dish; the bottom of the fixing groove is provided with a gas circulation hole.

7. The integrated device for precise culture and rapid detection of temperature-sensitive microorganisms according to claim 1, characterized in that: The control terminal is a touch screen display; the touch screen display has a built-in data storage module; the touch screen display is equipped with a USB interface for exporting data.

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