Automated nucleic acid extraction and purification instrument for single-portion processing

Abstract

The utility model discloses an automatic nucleic acid extraction and purification instrument can handle single person's portion, including casing, bearing frame, drive structure and magnetic sleeve frame, the bottom of casing is provided with nucleic acid extraction and purification operation platform, and a plurality of loading positions are arranged on nucleic acid extraction and purification operation platform, each loading position is used for loading reagent strip, and a plurality of reagent strips are independently arranged and can be detachably installed in the loading position, bearing frame is arranged on nucleic acid extraction and purification operation platform, and is arranged corresponding to a plurality of reagent strips, and a plurality of bearing grooves are set up on bearing frame, and each bearing groove is used for bearing magnetic bar sleeve, and a plurality of magnetic bar sleeves are independently arranged and can be detachably installed in the bearing groove, and a plurality of magnetic bars are arranged on the magnetic sleeve frame. The utility model discloses through reagent strip and magnetic bar sleeve independently arranged and detachably designed, so that the operator can assemble the required number of reagent strips and magnetic bar sleeves according to the sample quantity, without consuming the whole set of supporting consumables, effectively avoiding the waste of consumables under the small flux detection scene.

Description

Technical Field

[0001] This utility model relates to the field of purification instrument technology, and in particular to an automated nucleic acid extraction and purification instrument capable of processing single samples. Background Technology

[0002] Nucleic acid purification refers to the process of extracting and separating high-purity DNA or RNA from biological samples (such as blood, tissues, cells, viruses, or bacteria). Its purpose is to remove interfering components such as proteins, polysaccharides, lipids, and small molecule impurities, providing a reliable nucleic acid template for subsequent experiments such as PCR, sequencing, cloning, qPCR, and molecular diagnostics. Nucleic acid purification techniques typically include steps such as cell lysis, nucleic acid binding, washing, and elution. Currently, the main methods include centrifugation column methods, magnetic bead methods, and phenol / chloroform methods.

[0003] The magnetic bead method is widely used because it is simple and suitable for integration with automated equipment. However, in the existing technology, nucleic acid extraction and purification instruments using the magnetic bead method usually have reagent strips and magnetic rod sleeves set together, and the throughput is mostly between 48 and 96 samples. This results in the waste of the entire set of reagents and magnetic rod sleeves when dealing with a small number of samples, thus causing a lot of waste. Utility Model Content

[0004] The main purpose of this invention is to provide an automated nucleic acid extraction and purification instrument that can process samples by a single person, in order to solve the problem that the reagent strips and magnetic rod sleeves in the prior art are set up as a whole and cannot meet the testing needs of a small number of samples.

[0005] To achieve the above objectives, this utility model provides an automated nucleic acid extraction and purification instrument capable of processing single-person samples, comprising: The housing has a nucleic acid extraction and purification operating table at its bottom. The nucleic acid extraction and purification operating table has multiple loading positions spaced apart. Each loading position is used to load reagent strips. Multiple reagent strips are independently set and can be detachably installed in the loading position. A support frame is disposed on the nucleic acid extraction and purification operating table and corresponds to multiple reagent strips. The support frame has multiple support slots, the number of which corresponds to the number of loading positions. Each support slot is used to hold a magnetic rod sleeve, and multiple magnetic rod sleeves are independently disposed and detachably installed within the support slot. A magnetic sleeve holder is provided with multiple magnetic rods, the number of which corresponds to the number of reagent strips. A driving structure is connected to the magnetic sleeve and is used to drive the magnetic sleeve to move laterally or longitudinally, so that the magnetic rod can move to the corresponding support groove to grasp the magnetic rod sleeve, or move to the corresponding loading position to extract and purify the sample in the reagent strip.

[0006] Preferably, each reagent strip includes a pyrolysis tank, a first rinsing tank, a second rinsing tank, and an elution tank arranged sequentially and independently of each other. The pyrolysis tank is encapsulated with pyrolysis solution and magnetic beads. The first rinsing tank, the second rinsing tank, and the elution tank are respectively encapsulated with a first rinsing solution, a second rinsing solution, and an elution solution. The driving structure is used to drive the magnetic rod to grasp the magnetic rod sleeve and sequentially extend into the pyrolysis tank, the first rinsing tank, the second rinsing tank and the elution tank to extract and purify the sample in the reagent strip.

[0007] Preferably, each of the reagent strips is provided with a sealing film, which seals the openings of the lysis tank, the first rinsing tank, the second rinsing tank, and the elution tank.

[0008] Preferably, each of the loading positions is provided with a heat transfer sleeve, and the bottom of the reagent strip is provided with an insertion hole. The heat transfer sleeve is used to insert and cooperate with the insertion hole to install the reagent strip in the loading position. The heat transfer sleeve is also used to heat the reagent strip.

[0009] Preferably, the nucleic acid extraction and purification operating table is provided with two rows of loading positions spaced apart along a first direction, each row of loading positions includes eight loading positions spaced apart along a second direction, the first direction and the second direction are different, the support frame includes two sub-frames spaced apart along the first direction, each sub-frame is provided with eight support slots spaced apart along the second direction.

[0010] Preferably, the two sub-racks are respectively arranged on the side of the two rows of loading positions near the pyrolysis tank. The magnetic sleeve frame is provided with two rows of magnetic bars spaced apart along the first direction. Each row of magnetic bars includes eight magnetic bars spaced apart along the second direction, so that when the magnetic sleeve frame is moved to the point where any magnetic bar corresponds to the magnetic bar sleeve or the reagent strip, the remaining magnetic bars are all arranged in a one-to-one correspondence with the magnetic bar sleeve or the reagent strip.

[0011] Preferably, the reagent strip is recessed inward at both ends along the second direction corresponding to the first rinsing tank and the second rinsing tank, so that the outer wall of the reagent strip forms a gripping part.

[0012] Preferably, the magnetic sleeve frame is provided with a magnetic sleeve connector strip corresponding to each magnetic rod, which is sleeved on the outer periphery of the magnetic rod. The outer periphery of the magnetic sleeve connector strip is provided with a snap-fit ​​groove. The inner side of the magnetic rod sleeve near its opening is provided with a snap-fit ​​ring. The snap-fit ​​groove is used to snap-fit ​​with the snap-fit ​​ring to install the magnetic rod sleeve on the magnetic sleeve frame, and the magnetic rod is used to extend into the magnetic rod sleeve when the snap-fit ​​groove and the snap-fit ​​ring are snap-fitted together.

[0013] Preferably, the driving structure includes an X-axis driving module and a Z-axis driving module. The X-axis driving module is used to drive the magnetic rod to move horizontally so that the magnetic rod moves to the position corresponding to the magnetic rod sleeve or the reagent strip. The Z-axis driving module is used to drive the magnetic rod to move vertically up and down so that the magnetic rod moves to be installed with the magnetic rod sleeve or extends into the reagent strip.

[0014] Preferably, the housing is enclosed, with an opening on one side. The nucleic acid extraction and purification operating table is slidably disposed at the bottom of the housing. A baffle is provided on the nucleic acid extraction and purification operating table corresponding to the opening. A gripper is provided on the outside of the baffle, which is used to slide the nucleic acid extraction and purification operating table outside the housing when pulled.

[0015] In this invention, the nucleic acid extraction and purification operating table is equipped with multiple independent loading positions, each capable of individually installing or removing the corresponding reagent strip. The support frame has multiple corresponding support slots, each capable of independently placing or removing magnetic rod sleeves. Before testing, the required number of reagent strips is selected, the sample is injected into the strip, and the strip is installed in the loading position. Then, the corresponding number of magnetic rod sleeves are installed. Driven by a driving structure, the multiple magnetic rods on the magnetic sleeve frame can be positioned to one or more loading positions and support slots as needed, achieving selective extraction and purification of single or any number of samples. This invention, with its independently set and detachable design for both reagent strips and magnetic rod sleeves, allows operators to assemble only the required number of reagent strips and magnetic rod sleeves based on the sample quantity, eliminating the need to consume a complete set of consumables and effectively avoiding waste of consumables in low-throughput testing scenarios. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of the structure of an automated nucleic acid extraction and purification instrument according to an embodiment of the present invention; Figure 2 This is a schematic diagram of the internal structure of an automated nucleic acid extraction and purification instrument according to an embodiment of the present invention; Figure 3 This is a schematic diagram of the nucleic acid extraction and purification operating table and support frame of an automated nucleic acid extraction and purification instrument according to an embodiment of the present invention; Figure 4This is a schematic diagram of the magnetic sleeve of an automated nucleic acid extraction and purification instrument according to an embodiment of the present invention; Figure 5 This is a schematic diagram of the magnetic rod sleeve of an automated nucleic acid extraction and purification instrument according to an embodiment of the present invention; Figure 6 This is a schematic diagram of the magnetic rod and magnetic rod sleeve of an automated nucleic acid extraction and purification instrument according to an embodiment of the present invention.

[0018] Explanation of icon numbers: 100. Automated Nucleic Acid Extraction and Purification Instrument; 1. Shell; 11. Nucleic Acid Extraction and Purification Operating Table; 111. Baffle; 112. Gripper; 12. Loading Position; 121. Heat Transfer Jacket; 13. Opening; 2. Support Frame; 21. Support Tank; 22. Sub-frame; 3. Magnetic Sleeve Frame; 31. Magnetic Rod; 32. Magnetic Sleeve Connector Strip; 33. Snap-fit ​​Slot; 4. Drive Structure; 41. X-axis Drive Module; 42. Z-axis Drive Module; 5. Reagent Strip; 51. Lysis Tank; 52. First Rinse Tank; 53. Second Rinse Tank; 54. Elution Tank; 55. Gripper; 6. Magnetic Rod Sleeve; 61. Snap-fit ​​Ring.

[0019] The purpose, features, and advantages of this utility model will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0020] The technical solutions in this embodiment will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0021] It should be noted that all directional indicators (such as up, down, left, right, front, back, etc.) in this embodiment are only used to explain the relative positional relationship and movement of each component in a specific posture (as shown in the attached figure). If the specific posture changes, the directional indicator will also change accordingly.

[0022] Furthermore, in this utility model, the use of terms such as "first," "second," etc., is for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this utility model, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0023] In this utility model, unless otherwise explicitly specified and limited, the terms "connection," "fixing," etc., should be interpreted broadly. For example, "fixing" can mean a fixed connection, a detachable connection, or an integral part; it can mean a mechanical connection or an electrical connection; it can mean a direct connection or an indirect connection through an intermediate medium; it can mean the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0024] Furthermore, the technical solutions of the various embodiments of this utility model can be combined with each other, but only if they are feasible to those skilled in the art. If the combination of technical solutions contradicts each other or cannot be implemented, such a combination should be considered non-existent and not within the scope of protection claimed by this utility model. It should be understood that the specific embodiments described herein are merely illustrative of this utility model and are not intended to limit it.

[0025] In existing technologies, nucleic acid extraction and purification instruments using magnetic beads typically use reagent strips and magnetic rod sleeves as a set, and the throughput is mostly between 48 and 96 samples. This results in the waste of the entire set of reagents and magnetic rod sleeves when dealing with a small number of samples, causing significant waste.

[0026] To solve the above problems, please combine... Figures 1 to 4 This utility model proposes an automated nucleic acid extraction and purification instrument 100, including a housing 1, a support frame 2, a drive structure 4, and a magnetic sleeve 3. A nucleic acid extraction and purification operating table 11 is provided at the bottom of the housing 1. Multiple loading positions 12 are spaced apart on the operating table 11, each loading position 12 for loading reagent strips 5. The multiple reagent strips 5 are independently arranged and detachably installed within the loading position 12. The support frame 2 is disposed on the nucleic acid extraction and purification operating table 11 and corresponds to the multiple reagent strips 5. Multiple support grooves 21 are formed on the support frame 2. The number of carrier slots 21 is consistent with the number of loading positions 12 and is set one-to-one. Each carrier slot 21 is used to carry magnetic rod sleeves 6. Multiple magnetic rod sleeves 6 are set independently and can be detachably installed in the carrier slot 21. Multiple magnetic rods 31 are set on the magnetic sleeve frame 3. The number of magnetic rods 31 is consistent with the number of reagent strips 5. The driving structure 4 is connected to the magnetic sleeve frame 3 and is used to drive the magnetic sleeve frame 3 to move laterally or longitudinally, so that the magnetic rods 31 can move to the corresponding carrier slot 21 to grab the magnetic rod sleeves 6, or move to the corresponding loading position 12 to extract and purify the sample in the reagent strip 5.

[0027] In this invention, the nucleic acid extraction and purification operating table 11 is equipped with multiple independent loading positions 12, each of which can be individually installed or removed from the corresponding reagent strip 5. The support frame 2 is equipped with multiple corresponding support slots 21, each of which can be independently placed or removed from the magnetic rod sleeve 6. Before testing, the required number of reagent strips 5 are selected, the test sample is injected into the reagent strip 5, and then the reagent strip 5 is installed in the loading position 12. Then, the corresponding number of magnetic rod sleeves 6 are installed. Driven by the driving structure 4, the multiple magnetic rods 31 on the magnetic sleeve frame 3 can be positioned to one or more loading positions 12 and support slots 21 as needed, achieving selective extraction and purification of a single or any number of samples. This invention, through the independent and detachable design of both the reagent strips 5 and magnetic rod sleeves 6, allows operators to install only the required number of reagent strips 5 and magnetic rod sleeves 6 according to the sample quantity, without consuming the entire set of consumables, effectively avoiding reagent waste during low-throughput testing.

[0028] In one embodiment, each reagent strip 5 includes a lysis tank 51, a first rinsing tank 52, a second rinsing tank 53, and an elution tank 54 arranged sequentially and independently. The lysis tank 51 contains lysis buffer and magnetic beads. The first rinsing tank 52, the second rinsing tank 53, and the elution tank 54 contain a first rinsing solution, a second rinsing solution, and an elution solution, respectively. The driving structure 4 is used to drive the magnetic rod 31 to grasp the magnetic rod sleeve 6 and sequentially extend it into the lysis tank 51, the first rinsing tank 52, the second rinsing tank 53, and the elution tank 54 to extract and purify the sample in the reagent strip 5.

[0029] By pre-encapsulating the lysis buffer and magnetic beads within the lysis tank 51, the purification efficiency is improved by simply injecting the test sample into the lysis tank 51 during use, eliminating the need for additional steps. Each reagent strip 5 is equipped with a sequentially arranged and independent lysis tank 51, first rinsing tank 52, second rinsing tank 53, and elution tank 54, each encapsulated with its corresponding lysis buffer and magnetic beads, first rinsing solution, second rinsing solution, and elution solution. Each reagent strip 5 thus constitutes a complete nucleic acid extraction and purification process unit. The drive structure 4 drives the magnetic rod 31 to grasp the magnetic rod sleeve 6 and sequentially insert it into the lysis tank 51, first rinsing tank 52, second rinsing tank 53, and elution tank 54, thereby achieving fully automated nucleic acid extraction and purification of the sample within a single reagent strip 5.

[0030] In one embodiment, each reagent strip 5 is provided with a sealing film that seals the openings of the lysis tank 51, the first rinsing tank 52, the second rinsing tank 53, and the elution tank 54. The sealing film effectively isolates air, humidity, and external contaminants, ensuring the chemical stability of the lysis buffer, the first rinsing buffer, the second rinsing buffer, and the elution buffer during storage, thus improving the shelf life and reliability of the reagent strip 5. Furthermore, since multiple tanks share a single sealing film, opening all tank openings can be achieved simply by tearing off the sealing film, further improving detection efficiency.

[0031] In one embodiment, a heat transfer sleeve 121 is provided at each of the loading positions 12, and a plug hole is provided at the bottom of the reagent strip 5. The heat transfer sleeve 121 is used to plug into the plug hole to install the reagent strip 5 at the loading position 12. The heat transfer sleeve 121 is also used to heat the reagent strip 5.

[0032] The heat transfer sleeve 121 is inserted into the bottom of the reagent strip 5 through the insertion hole, allowing the reagent strip 5 to be accurately aligned and securely installed at the loading position 12. This prevents the reagent strip 5 from tilting, shaking, or shifting due to inaccurate manual placement, thus improving the mechanical precision of automated operation. By setting the heat transfer sleeve 121 at each loading position 12 and heating the lysis buffer, first wash buffer, second wash buffer, and elution buffer inside the reagent strip 5 through the heat transfer sleeve 121, each key step in the nucleic acid extraction and purification process can be carried out at the optimal temperature, improving purification accuracy and efficiency.

[0033] In one embodiment, the nucleic acid extraction and purification operating table 11 is provided with two rows of loading positions 12 spaced apart along a first direction. Each row of loading positions 12 includes eight loading positions 12 spaced apart along a second direction. The first direction and the second direction are different. The support frame 2 includes two sub-frames 22 spaced apart along the first direction. Each sub-frame 22 is provided with eight support slots 21 spaced apart along the second direction.

[0034] It should be noted that the first direction is Figure 1 The left and right directions, the second direction is Figure 1 The front-back direction in the text, and the direction in which the nucleic acid extraction and purification operating table 11 is pulled in the following text is also the second direction. The loading position 12 and the carrier tank 21 form a regular matrix correspondence in spatial layout, making the mechanical path of the whole device shorter and more regular in the horizontal and vertical directions. This helps to improve the operating efficiency of the drive structure 4 and reduce positioning errors, thereby improving the overall speed and stability of nucleic acid extraction and purification. Moreover, the two-row layout is suitable for processing a small number of reagent strips 5 at one time (for example, using only one loading position 12) or for processing sixteen reagent strips 5 at the same time, realizing flexible switching between low throughput and medium throughput. This further enhances the adaptability of this application to different small sample quantities, taking into account the needs of single-person and small-batch testing.

[0035] In one embodiment, two sub-racks 22 are respectively arranged on the side of the two rows of loading positions 12 near the pyrolysis tank 51. The magnetic sleeve frame 3 is provided with two rows of magnetic rods 31 spaced apart along the first direction. Each row of magnetic rods 31 includes eight magnetic rods 31 spaced apart along the second direction, so that when the magnetic sleeve frame 3 moves to the point where any magnetic rod 31 corresponds to the magnetic rod sleeve 6 or reagent strip 5, the remaining magnetic rods 31 are all arranged in a one-to-one correspondence with the magnetic rod sleeve 6 or reagent strip 5.

[0036] The two rows of magnetic rods 31 correspond strictly one-to-one with the two rows of loading positions 12 and the bearing grooves 21 on the two sub-frames 22. This ensures that when the magnetic sleeve frame 3 moves to any position under the drive structure 4, the target magnetic rod 31 is perfectly aligned with the target magnetic rod sleeve 6 or reagent strip 5. This eliminates the need for complex offset compensation, improving the accuracy and stability of the insertion, extraction, and movement of the magnetic rods 31. When the magnetic sleeve frame 3 moves to a point where a certain magnetic rod 31 is aligned with the corresponding magnetic rod sleeve 6 or reagent strip 5, the remaining magnetic rods 31 still maintain a one-to-one correspondence with the corresponding magnetic rod sleeve 6 or reagent strip 5. This ensures that the mechanical system maintains consistent coordinates regardless of whether the equipment processes a single sample or multiple samples, reducing operational errors and improving the reliability of automated control.

[0037] In one embodiment, the reagent strip is recessed inward at both ends along the second direction at the first rinsing tank 52 and the second rinsing tank 53, so that the outer wall of the reagent strip forms a gripping portion 55.

[0038] The gripping part 55 forms a distinct recessed area, making it easier for operators to grip the reagent strip 5 when installing it into or removing it from the loading position 12, reducing finger slippage or poor gripping, and significantly improving the operating experience.

[0039] Please combine Figure 5 and Figure 6 In one embodiment, the magnetic sleeve frame 3 is provided with a magnetic sleeve connector strip 32 that is sleeved on the outer periphery of each magnetic rod 31. The outer periphery of the magnetic sleeve connector strip 32 is provided with a snap-fit ​​groove 33. The inner side of the magnetic rod sleeve 6 near its opening 13 is provided with a snap-fit ​​ring 61. The snap-fit ​​groove 33 is used to snap-fit ​​with the snap-fit ​​ring 61 to install the magnetic rod sleeve 6 on the magnetic sleeve frame 3. The magnetic rod 31 is used to extend into the magnetic rod sleeve 6 when the snap-fit ​​groove 33 snaps with the snap-fit ​​ring 61.

[0040] The snap-fit ​​groove 33 and the snap-fit ​​ring 61 form a reliable snap-fit ​​engagement, allowing the magnetic rod sleeve 6 to be quickly snapped in or removed without the use of additional tools, significantly shortening the operation time for replacing the magnetic rod sleeve 6 and improving ease of use.

[0041] In one embodiment, the drive structure 4 includes an X-axis drive module 41 and a Z-axis drive module 42. The X-axis drive module 41 is used to drive the magnetic rod 31 to move horizontally so that the magnetic rod 31 moves to the corresponding magnetic rod sleeve 6 or the corresponding reagent strip 5. The Z-axis drive module 42 is used to drive the magnetic rod 31 to move vertically so that the magnetic rod 31 is installed with the magnetic rod sleeve 6 or extends into the reagent strip 5.

[0042] By eliminating the Y-axis drive module, only the X-axis and Z-axis drive modules are needed to cover all the arrangement positions of the magnetic rod sleeve 6 and reagent strip 5. This simplifies the drive structure 4, reduces the number of moving parts, thereby lowering the failure rate and improving the overall reliability of the equipment. The X-axis drive module is responsible for all horizontal movement, ensuring that the magnetic rod 31 moves along a single, highly controllable trajectory in the horizontal direction. This helps improve the positioning accuracy of the magnetic rod 31 when it moves to the position of the magnetic rod sleeve 6 or reagent strip 5, reducing offset or error.

[0043] In one embodiment, the housing 1 is closed, and an opening 13 is provided on one side of the housing 1. The nucleic acid extraction and purification operating table 11 is slidably disposed at the bottom of the housing 1. A baffle 111 is provided on the nucleic acid extraction and purification operating table 11 at the position corresponding to the opening 13. A gripper 112 is provided on the outside of the baffle 111. The gripper 112 is used to drive the nucleic acid extraction and purification operating table 11 to slide outside the housing 1 when it is pulled.

[0044] A handle 112 is located on the outside of the baffle 111, allowing operators to directly pull the handle 112 to smoothly slide the nucleic acid extraction and purification operating table 11 from inside the housing 1 to the outside. This facilitates the rapid loading of reagent strips 5 and magnetic rod sleeves 6 without bending over or reaching into the housing 1, significantly improving the operating experience. The housing 1 is enclosed, helping to prevent external dust, liquid splashes, or environmental contaminants from entering the reagent strip 5 area inside the housing 1, improving the cleanliness and reliability of the nucleic acid extraction and purification process. The nucleic acid extraction and purification operating table 11 slides out only when loading and unloading are needed, achieving a balance between a closed structure and ease of operation.

[0045] The above are merely preferred embodiments of this utility model and do not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the description and drawings of this utility model, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.

Claims

1. An automated nucleic acid extraction and purification instrument, characterized in that, include: The housing has a nucleic acid extraction and purification operating table at its bottom. The nucleic acid extraction and purification operating table has multiple loading positions spaced apart. Each loading position is used to load reagent strips. Multiple reagent strips are independently set and can be detachably installed in the loading position. A support frame is provided on the nucleic acid extraction and purification operating table and is provided for multiple reagent strips. The support frame has multiple support slots, the number of which is the same as the number of loading positions and is provided one-to-one. Each support slot is used to support the magnetic rod sleeve. A magnetic sleeve holder, wherein a plurality of magnetic bars are provided on the magnetic sleeve holder, the number of magnetic bars being the same as the number of reagent strips; A driving structure is connected to the magnetic sleeve and is used to drive the magnetic sleeve to move laterally or longitudinally, so that the magnetic rod can move to the corresponding support groove to grasp the magnetic rod sleeve, or move to the corresponding loading position to extract and purify the sample in the reagent strip.

2. The automated nucleic acid extraction and purification instrument as described in claim 1, characterized in that, Each of the reagent strips includes a lysis tank, a first rinsing tank, a second rinsing tank, and an elution tank arranged sequentially and independently of each other. The lysis tank is encapsulated with lysis solution and magnetic beads. The first rinsing tank, the second rinsing tank, and the elution tank are respectively encapsulated with a first rinsing solution, a second rinsing solution, and an elution solution. The driving structure is used to drive the magnetic rod to grasp the magnetic rod sleeve and sequentially extend into the pyrolysis tank, the first rinsing tank, the second rinsing tank and the elution tank to extract and purify the sample in the reagent strip.

3. The automated nucleic acid extraction and purification instrument as described in claim 2, characterized in that, Each of the reagent strips is provided with a sealing film, which seals the openings of the lysis tank, the first rinsing tank, the second rinsing tank, and the elution tank.

4. The automated nucleic acid extraction and purification instrument as described in claim 2, characterized in that, A heat transfer sleeve is provided at each of the loading positions, and an insertion hole is provided at the bottom of the reagent strip. The heat transfer sleeve is used to insert and cooperate with the insertion hole to install the reagent strip at the loading position. The heat transfer sleeve is also used to heat the reagent strip.

5. The automated nucleic acid extraction and purification instrument as described in claim 2, characterized in that, The nucleic acid extraction and purification operating table is provided with two rows of loading positions spaced apart along a first direction. Each row of loading positions includes eight loading positions spaced apart along a second direction. The first direction and the second direction are different. The support frame includes two sub-frames spaced apart along the first direction. Each sub-frame is provided with eight support slots spaced apart along the second direction.

6. The automated nucleic acid extraction and purification instrument as described in claim 5, characterized in that, The two sub-racks are respectively arranged on the side of the two rows of loading positions near the pyrolysis tank. The magnetic sleeve frame is provided with two rows of magnetic bars spaced apart along the first direction. Each row of magnetic bars includes eight magnetic bars spaced apart along the second direction, so that when the magnetic sleeve frame is moved to the point where any magnetic bar corresponds to the magnetic bar sleeve or the reagent strip, the remaining magnetic bars are all arranged to correspond one-to-one with the magnetic bar sleeve or the reagent strip.

7. The automated nucleic acid extraction and purification instrument as described in claim 5, characterized in that, The reagent strip is recessed inward at both ends along the second direction at the locations corresponding to the first and second rinsing tanks, so that the outer wall of the reagent strip forms a gripping portion.

8. The automated nucleic acid extraction and purification instrument according to any one of claims 1 to 7, characterized in that, The magnetic sleeve frame is provided with a magnetic sleeve connector strip that is sleeved on the outer periphery of each magnetic rod. The outer periphery of the magnetic sleeve connector strip is provided with a snap-fit ​​groove. The inner side of the magnetic rod sleeve near its opening is provided with a snap-fit ​​ring. The snap-fit ​​groove is used to snap-fit ​​with the snap-fit ​​ring to install the magnetic rod sleeve on the magnetic sleeve frame. The magnetic rod is used to extend into the magnetic rod sleeve when the snap-fit ​​groove and the snap-fit ​​ring are snap-fit.

9. The automated nucleic acid extraction and purification instrument according to any one of claims 1 to 7, characterized in that, The driving structure includes an X-axis driving module and a Z-axis driving module. The X-axis driving module is used to drive the magnetic rod to move horizontally so that the magnetic rod moves to the position corresponding to the magnetic rod sleeve or the reagent strip. The Z-axis driving module is used to drive the magnetic rod to move vertically up and down so that the magnetic rod moves to be installed with the magnetic rod sleeve or extends into the reagent strip.

10. The automated nucleic acid extraction and purification instrument according to any one of claims 1 to 7, characterized in that, The housing is enclosed, with an opening on one side. The nucleic acid extraction and purification operating table is slidably disposed at the bottom of the housing. A baffle is provided on the nucleic acid extraction and purification operating table corresponding to the opening. A gripper is provided on the outside of the baffle, which is used to slide the nucleic acid extraction and purification operating table outside the housing when pulled.

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