A denitrifying bacteria solid state fermentation device

Abstract

The utility model relates to sewage treatment technical field, concretely is a kind of denitrifying bacteria solid state fermentation device, the utility model discloses a fermentation container body, the fermentation container is stainless steel cylinder jar, including stirring device, temperature and humidity control system, ventilation system, the stirring system of this device uses the radial flow type stirrer of upper layer and the axial flow type stirrer of lower layer cooperation stirring, fully utilizes the circulating ability of axial flow type stirrer and radial flow type stirrer's shearing and dispersion ability, improves the mixing effect of material, and can adjust stirring speed according to needs, ensure that material is evenly mixed in jar body, improve fermentation efficiency;Temperature and humidity control system can monitor and adjust the temperature and humidity in jar body in real time, keep its stability and suitability, conducive to the growth and metabolism of denitrifying bacteria, and ventilation system is equipped with air filter device, ensure that the air clean and sterile entering jar body, reduce the possibility of miscellaneous bacteria pollution, improve experimental efficiency.

Description

Technical Field

[0001] This invention relates to the field of wastewater treatment technology, specifically to a solid-state fermentation device for denitrifying bacteria. Background Technology

[0002] With the acceleration of urbanization and the rapid expansion of urban populations, the discharge of municipal and industrial wastewater continues to rise. This massive wastewater discharge places enormous pressure on the environment, making wastewater treatment a crucial task for protecting the urban environment and residents' health. Wastewater treatment technology has evolved from the traditional activated sludge process to modern biofilm processes, oxidation ditch processes, and SBR processes. In recent years, wastewater treatment technologies have seen continuous innovation, such as the IBR continuous flow integrated intermittent activated sludge process, the distributed vertical circulation enhanced denitrification and phosphorus removal technology, the high-efficiency self-priming mixed inverted umbrella aerator, and the SPR high-turbidity wastewater purification system. These technologies not only represent the latest advancements in the field of wastewater treatment but also provide new ideas and methods for solving water pollution problems.

[0003] Wastewater treatment methods are diverse, including physical, chemical, and biological treatment. Physical treatment mainly removes suspended solids through methods such as sieving and sedimentation; chemical treatment removes dissolved pollutants through methods such as coagulation and neutralization; and biological treatment utilizes microorganisms to degrade organic matter. In addition, there are advanced oxidation technologies such as micro-electrolysis and ozone oxidation, as well as new technologies from abroad such as heavy metal filters made from plant waste. Denitrifying bacteria are a type of microorganism that can reduce nitrate nitrogen to nitrogen gas, playing a crucial role in wastewater treatment. However, the growth and activity of denitrifying bacteria are affected by various factors, such as temperature, humidity, and oxygen content. To improve the activity and production efficiency of denitrifying bacteria, a solid-state fermentation device for denitrifying bacteria has been developed. Summary of the Invention

[0004] This invention provides a solid-state fermentation device for denitrifying bacteria to solve the problems existing in the prior art.

[0005] The technical solutions adopted in this utility model are as follows: A solid-state fermentation device for denitrifying bacteria includes a fermentation container body, which is a stainless steel cylindrical tank. The fermentation container includes a stirring device, a temperature and humidity control system, and a ventilation system. The stirring device is equipped with an upper radial flow stirrer and a lower axial flow stirrer.

[0006] Preferably, the stirring device further includes a stirring shaft, a first flange is provided between the radial flow type stirrer and the axial flow type stirrer, and a motor is provided on the top of the fermentation vessel and connected to the stirring shaft through a second flange.

[0007] Preferably, the connecting first flange includes a raised face flange and a recessed face flange, and a reamed bolt hole is provided perpendicular to the direction of the first flange.

[0008] Preferably, the temperature and humidity control system includes temperature and humidity sensors and is equipped with a brewing belt on the outside of the tank.

[0009] Preferably, the ventilation system includes several exhaust ports and is equipped with plugs, and the ventilation system is also equipped with an air filter.

[0010] Preferably, the fermentation container further includes a feed inlet, a water distributor, and a discharge outlet, and the feed inlet is provided with a handle.

[0011] Preferably, the brewing belt outside the fermentation vessel is a liquid medium.

[0012] Preferably, the culture medium used for fermentation in this device is a solid culture medium containing abundant carbon source, nitrogen source, trace elements and carrier. The solid culture medium carrier is wheat bran, the carbon source is glucose, the nitrogen source is potassium nitrate, and the trace elements include phosphorus, iron, magnesium, etc.

[0013] Compared with the prior art, the beneficial effects of this utility model are as follows: The mixing system of this apparatus employs a combination of an upper radial flow mixer and a lower axial flow mixer. This fully utilizes the circulation capacity of the axial flow mixer and the shearing and dispersing capabilities of the radial flow mixer, improving the mixing effect of the materials. The mixing speed can be adjusted as needed to ensure uniform mixing within the tank, thus enhancing fermentation efficiency. The ventilation system precisely controls the oxygen concentration within the fermentation vessel by opening and closing blockages, providing a more precisely controlled micro-aerobic environment for denitrifying bacteria. The temperature and humidity control system monitors and adjusts the temperature and humidity within the tank in real time, maintaining their stability and suitability, which is beneficial for the growth and metabolism of denitrifying bacteria. Furthermore, the ventilation system is equipped with an air filter to ensure that the air entering the tank is clean and sterile, reducing the possibility of contamination and improving experimental efficiency. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall design of this utility model. Figure 2 This is a partially enlarged view of the first flange of this utility model. In the diagram: 1-Fermentation container, 3-Agitator, 4-Temperature and humidity control system, 101-Feed inlet, 102-Water distributor, 104-Handle, 106-Discharge port, 301-Agitator shaft, 302-Radial flow agitator, 303-Axial flow agitator, 304-Raised face flange, 305-Concave face flange, 306-First flange, 307-Second flange, 308-Reamed hole bolt, 401-Brewing belt, 501-Exhaust port, 502-Block. Detailed Implementation

[0015] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0016] A solid-state fermentation device for denitrifying bacteria includes a fermentation container 1 body, wherein the fermentation container 1 is a stainless steel cylindrical tank, and includes a stirring device 3, a temperature and humidity control system 4, and a ventilation system.

[0017] The stirring device 3 includes a stirring shaft 301, an upper radial flow stirrer 302 and a lower axial flow stirrer 303, making full use of the circulation capacity of the axial flow stirrer and the shearing and dispersing capacity of the radial flow stirrer. A first flange 306 is provided between the radial flow stirrer 302 and the axial flow stirrer 303. A motor is provided on the top of the fermentation container 1 and connected to the stirring shaft 301 through a second flange 307.

[0018] The upper radial flow agitator 302 provides radial flow, enhancing the shearing and dispersion of materials and providing high shear force for denitrifying bacteria solid-state fermentation.

[0019] The lower axial flow agitator 303 circulates the material axially. The high-speed rotation generates a strong axial flow, causing the material to circulate up and down in the fermenter and preventing the material from settling.

[0020] The connection between the first flange 306 and the second flange 307 includes a raised face flange 304 and a concave face flange 305. In order to reduce the impact load on the connecting bolts of the first flange 306 at the moment of start-up, a reamed bolt 308 is provided in the direction perpendicular to the first flange 306.

[0021] The temperature and humidity control system 4 includes temperature and humidity sensors and is equipped with a brewing belt 401 on the outside of the tank to maintain a suitable fermentation temperature by heating or cooling.

[0022] The ventilation system includes several exhaust ports 501. By precisely controlling the size of the exhaust ports, the number of open and closed exhaust ports, and the air flow, it is convenient to discharge the gases generated during the fermentation process and provide a precisely controlled micro-oxygen environment for denitrifying bacteria. It also controls the air circulation and humidity level inside the tank. The ventilation system is also equipped with an air filtration device to ensure that the air entering the tank is clean and sterile.

[0023] The fermentation container 1 also includes a feed inlet 101, a water distributor 102, and a discharge outlet 106, and the feed inlet is provided with a handle 104.

[0024] The brewing belt 401 outside the fermentation container 1 is a liquid medium.

[0025] The fermentation medium used in this device is a solid medium containing abundant carbon source, nitrogen source, trace elements and carrier. The solid medium carrier is wheat bran, the carbon source is glucose, the nitrogen source is potassium nitrate, and the trace elements include phosphorus, iron, magnesium, etc. Example

[0026] 1. Experimental Preparation Culture medium preparation: Prepare solid culture media containing abundant carbon sources, nitrogen sources, trace elements, and carriers.

[0027] Mix the culture medium thoroughly to ensure that all components are evenly distributed.

[0028] Place the prepared solid culture medium in a container that is easy to put into the fermenter.

[0029] Equipment inspection: Check whether the fermentation tank and its auxiliary equipment (stirring device, temperature and humidity control system, ventilation system, etc.) are operating normally.

[0030] Ensure that all connections (such as flanges and bolts) are securely installed without any looseness or leakage.

[0031] Check that the motor, sensors, ventilation system, etc. are in good condition to ensure stable equipment operation during the experiment.

[0032] Safety precautions: Ensure that operators wear necessary protective equipment (such as gloves, goggles, etc.).

[0033] Check the ventilation in the experimental area to ensure a safe experimental environment.

[0034] 2. Fermentation tank filling Open the feed inlet: Open the feed inlet 101 of the fermentation tank and ensure that the feed inlet is well sealed and leak-free.

[0035] The prepared solid culture medium is poured evenly into the fermenter through the feed inlet.

[0036] Close the feed inlet: After pouring in the culture medium, immediately close the feed inlet and ensure a seal to prevent external contamination from entering the fermenter.

[0037] 3. Start the mixing system Connect the motor: Confirm that the motor at the top of the fermenter is securely connected to the stirring shaft 301 via the second flange 307.

[0038] Start the motor to make the stirring shaft start rotating.

[0039] Adjust the stirring speed: The stirring speed is adjusted by a frequency converter so that the upper radial flow mixer 302 and the lower axial flow mixer 303 can reach their optimal operating speeds.

[0040] Upper radial flow agitator 302: Provides radial flow, enhancing the shearing and dispersion of materials. The recommended rotation speed is 150±20 r / min.

[0041] Lower axial flow agitator 303: Provides axial circulation to prevent material settling; recommended speed setting is 100±10 r / min.

[0042] Monitor the mixing effect: Observe the mixing of materials during the stirring process to ensure there are no dead corners and uneven distribution.

[0043] If the mixing effect is not good, you can adjust the mixing speed or the angle of the mixer as appropriate.

[0044] 4. Start the temperature and humidity control system. Set temperature and humidity parameters: Start the temperature and humidity control system 4, and input the preset temperature (30±2℃) and humidity (55±5%) on the control panel.

[0045] The appropriate temperature inside the fermentation tank is maintained by heating or cooling through the brewing belt 401 outside the tank.

[0046] Monitor temperature and humidity: Temperature and humidity sensors are used to monitor the temperature and humidity inside the fermenter in real time to ensure that they meet the preset values.

[0047] If a large deviation in temperature and humidity is found, adjust the heating or cooling power of the brewing zone in a timely manner.

[0048] 5. Start the ventilation system. Adjust the air vents: According to the needs of the fermentation process, turn on the ventilation system and adjust the size of the vents, the number of vents that are open and closed, and the air flow.

[0049] The ventilation system provides a more precisely controlled micro-oxygen environment for denitrifying bacteria, controlling air circulation and humidity levels within the tank.

[0050] Check the air filter: Ensure that the air filtration device in the ventilation system is operating normally, and that the air entering the tank is clean and sterile.

[0051] Regularly check the operating status of the filter device and replace or clean the filter element in a timely manner.

[0052] Monitor ventilation effectiveness: Sensors are used to monitor the oxygen and carbon dioxide concentrations in the fermenter in real time to ensure that they meet the growth requirements of denitrifying bacteria.

[0053] If poor ventilation is found, the setting of the ventilation openings or the operating parameters of the ventilation system can be adjusted appropriately.

[0054] 6. Real-time monitoring and adjustment Monitoring the fermentation process: During the fermentation process, the control system monitors parameters such as temperature, humidity, oxygen concentration, and carbon dioxide concentration inside the fermenter in real time.

[0055] Record all data during the fermentation process for subsequent analysis.

[0056] Adjust fermentation conditions: Based on the monitoring results, parameters such as stirring speed, temperature, humidity, and ventilation are adjusted in a timely manner to ensure the stability and efficiency of the fermentation process.

[0057] 7. Fermentation complete Confirm fermentation is complete: Based on the preset fermentation time or monitoring results (such as the achievement of denitrification), it is determined that fermentation has been completed.

[0058] The fermentation effect can be judged by detecting indicators such as the nitrate nitrogen content and nitrogen generation in the fermenter.

[0059] Shutting down the system: The ventilation system, stirring system, and temperature and humidity control system were shut down in sequence to reduce the impact of vibration on the fermentation products.

[0060] Ensure all systems are completely shut down before proceeding with sampling and analysis.

[0061] Based on the analysis results, the fermentation effect was evaluated, and experimental experience was summarized to provide a reference for subsequent experiments. It will be apparent to those skilled in the art that this invention is not limited to the details of the above exemplary embodiments, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects. The scope of this invention is defined by the appended claims rather than the foregoing description. Therefore, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within this invention, and no reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A solid-state fermentation apparatus for denitrifying bacteria, comprising a fermentation vessel (1), characterized in that, The fermentation container (1) is a stainless steel cylindrical tank, including a stirring device (3), a temperature and humidity control system (4), and a ventilation system. The stirring device (3) is equipped with an upper radial flow stirrer (302) and a lower axial flow stirrer (303).

2. The denitrifying bacteria solid-state fermentation device according to claim 1, characterized in that, The stirring device (3) also includes a stirring shaft (301), a first flange (306) is provided between the radial flow type stirrer (302) and the axial flow type stirrer (303), and a motor is provided on the top of the fermentation container (1) and connected to the stirring shaft (301) through a second flange (307).

3. The denitrifying bacteria solid-state fermentation device according to claim 2, characterized in that, The first flange (306) includes a raised face flange (304) and a recessed face flange (305), and has a hinged hole bolt (308) perpendicular to the direction of the first flange.

4. The denitrifying bacteria solid-state fermentation device according to claim 1, characterized in that, The temperature and humidity control system (4) includes temperature and humidity sensors and is equipped with a brewing belt (401) outside the tank, in which liquid medium circulates.

5. The denitrifying bacteria solid-state fermentation device according to claim 1, characterized in that, The ventilation system (5) includes several exhaust ports (501) and is equipped with plugs (502). The ventilation system is also equipped with an air filter.

6. The denitrifying bacteria solid-state fermentation device according to claim 1, characterized in that, The fermentation container (1) also includes a feed inlet (101), a water distributor (102), and a discharge outlet (106), and the feed inlet is provided with a handle (104).

7. The denitrifying bacteria solid-state fermentation device according to claim 1, characterized in that, The fermentation container (1) is equipped with a brewing belt (401) on the outside of the tank, which is a liquid medium.

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