A cell recovery and counting integrated device

Abstract

The utility model discloses a cell recovery and counting integrated equipment relates to cell recovery and counting technical field. Including: device body, one end fixed mounting of device body has control panel, is used for the control of device and the display of device data, the top of device body is rotatably connected with the top cover, and the top of device body is provided with the water bath, and the inside equidistance of water bath is placed with the placing mechanism, and the sample test tube is inserted in the placing mechanism, and the inside bottom of water bath is installed with the stirring mechanism, the placing mechanism includes the bottom plate, and the top of bottom plate is equipped with two layering board. The utility model discloses the setting can be placed to sample test tube through the placing mechanism, and the independent placing chamber formed through layering board and the separate rack can be placed stably to sample test tube, in addition, the independent chamber can protect the sample test tube of the side in the case of sample test tube fragmentation, further improve the stability and security of sample test tube when heating.

Description

Technical Field

[0001] This invention belongs to the field of cell resuscitation and counting technology, and in particular relates to an integrated device for cell resuscitation and counting. Background Technology

[0002] Cell resuscitation is the process of restoring the activity of cryopreserved cells. It refers to the process of gradually thawing cells that have been frozen at low temperatures (such as -80°C freezers or liquid nitrogen) to restore their normal metabolic and proliferative capabilities. The core purpose is to "revive" dormant cells and reuse them for experiments or culture. Cell counting is the process of counting the number of live cells in a cell suspension. It involves using a counting chamber (such as a hemocytometer) or other instruments to count the total number of cells in the cell suspension, especially distinguishing between live and dead cells. Its core purpose is to determine the cell concentration and provide an accurate reference for the cell quantity in subsequent seeding, culture, and experimental processing.

[0003] During cell thawing and counting, residual liquid nitrogen in the cryovials can easily cause a heating explosion, and the lack of explosion protection design may damage other samples. This is not conducive to the thawing and counting process and to safe operation. Utility Model Content

[0004] The purpose of this invention is to provide an integrated cell resuscitation and counting device that solves the existing problems.

[0005] To solve the above-mentioned technical problems, this utility model is achieved through the following technical solution: This utility model is an integrated device for cell resuscitation and counting, comprising: a device body, a control panel fixedly installed at one end of the device body for controlling the device and displaying device data, a top cover rotatably connected to the top of the device body, a water bath tank opened at the top of the device body, a placement mechanism equidistantly placed inside the water bath tank, a sample tube inserted into the placement mechanism, and a stirring mechanism installed at the bottom of the water bath tank; the placement mechanism includes a base plate, two layered plates are provided above the base plate, the side walls of the base plate and the layered plates are provided with several side frames, and the side walls of the base plate and the layered plates are provided with snap-fit ​​seats that engage with the side frames.

[0006] Furthermore, the base plate has a number of rectangular perforations, and the surface of the layered plate has a number of insertion holes at equal intervals for inserting sample tubes. The insertion holes allow for the installation and placement of sample tubes, while the perforations in the base plate increase the flow of warm water at the bottom of the placement mechanism.

[0007] Furthermore, several side frames are U-shaped, which allows for the installation of the base plate and layered panels. In addition, the U-shaped side frames can reduce their own weight and production costs.

[0008] Furthermore, the placement mechanism also includes a partition frame respectively disposed between the base plate and the layered plates, and the partition frame is fixedly connected to the base plate and the layered plates respectively. The partition frame has several flow channels and several lightweight holes equally spaced on it. In order to increase the stability of the sample tubes during placement, the two layered plates can support the sample tubes. In addition, in order to prevent the breakage of adjacent sample tubes when a sample tube breaks, the partition frame between the base plate and the layered plates can separate several sample tubes. At the same time, multiple independent compartments are formed between the layered plates and the partition frame, which can better place the sample tubes and protect them, further improving the safety of the sample tubes during heating. In addition, in order to increase the flow of warm water inside the placement mechanism, the partition frame has flow channels, which can work with the stirring mechanism to increase the flow of warm water inside the independent compartments, better heating the sample tubes. The cooperation between the base plate and the side frame can support the layered plates, further improving the stability of the placement mechanism during use.

[0009] Furthermore, the bottom of the water bath is provided with several support bars at equal intervals, and the support bars are all wavy. The support bars can support the placement mechanism and prevent the placement mechanism from directly contacting the ground of the water bath. This can increase the flow of warm water at the bottom of the placement mechanism, further improving the heating speed of the sample tubes. The wavy support bars also facilitate the flow of warm water, further improving the practicality of the device.

[0010] Furthermore, the stirring mechanism includes mounting seats symmetrically fixed on both sides of the water bath. A rotating rod is provided between the two mounting seats, and both ends of the rotating rod are rotatably connected to the two mounting seats respectively. One end of the rotating rod extends into the device body and is fixedly connected to one end of the synchronous belt structure. A drive motor is fixedly installed inside the device body, and the output end of the drive motor is fixedly connected to the other end of the synchronous belt structure. Several stirring blades are fixedly installed at equal intervals on the surface of the rotating rod. When the sample tube is placed in the water bath through the placement mechanism, the temperature of the warm water around the sample tube will decrease. In order to reduce the fluctuation of the water temperature around the tube, the drive motor will drive the rotating rod to rotate through the synchronous belt structure. When the rotating rod rotates, it works with the stirring blades to stir the warm water in the water bath, mixing the warm water in the water bath and thus better heating the sample tube.

[0011] Furthermore, the surface of the stirring blade is provided with several through holes at equal intervals. The through holes on the surface of the stirring blade can reduce the fluctuation of the constant temperature water in the water bath when the stirring blade is stirring, and prevent splashing when the stirring mechanism is stirring, thereby further improving the stability of the device during use.

[0012] This utility model has the following beneficial effects: 1. This utility model can place sample tubes through the setting of the placement mechanism, and the independent placement chamber formed by the layered plate and the partition rack can stably place the sample tubes. In addition, the independent chamber can protect the sample tubes next to it in the event of sample tube breakage, further improving the stability and safety of sample tubes during heating.

[0013] 2. This utility model can agitate the warm water inside the water bath by setting a stirring mechanism, which can further improve the fluidity of the warm water in the water bath, so that the temperature of the warm water is kept the same and the sample tubes are heated better. In addition, in order to increase the fluidity of the warm water inside the placement mechanism, the setting of the diversion tank can flow the warm water, thereby better heating the sample tubes.

[0014] Of course, any product implementing this utility model does not necessarily need to achieve all of the advantages described above at the same time. Attached Figure Description

[0015] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 This is a first-person perspective schematic diagram of the overall device structure of this utility model; Figure 2 This is a second-view schematic diagram of the overall device structure of this utility model; Figure 3 This is a partial structural diagram of the device of this utility model; Figure 4 This is an exploded view of the placement mechanism of this utility model; Figure 5 This is a schematic diagram of the support strip structure of this utility model; Figure 6 This is a schematic diagram of the stirring mechanism of this utility model.

[0017] The attached diagram lists the components represented by each number as follows: 1. Device body; 2. Control panel; 3. Top cover; 4. Water bath; 5. Placement mechanism; 6. Stirring mechanism; 7. Base plate; 8. Layered plate; 9. Side frame; 10. Snap-fit ​​seat; 11. Insertion hole; 12. Divider frame; 13. Diversion channel; 14. Lightweight hole; 15. Support bar; 16. Mounting base; 17. Rotating rod; 18. Stirring blade; 19. Synchronous belt structure; 20. Drive motor. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0019] Please see Figure 1-6 This utility model is an integrated device for cell resuscitation and counting, comprising: The device body 1 has a control panel 2 fixedly installed at one end for controlling the device and displaying device data. A top cover 3 is rotatably connected to the top of the device body 1. A water bath 4 is provided at the top of the device body 1. Placement mechanisms 5 are placed equidistantly inside the water bath 4. Sample test tubes are inserted into the placement mechanisms 5. A stirring mechanism 6 is installed at the bottom of the water bath 4. The placement mechanism 5 includes a base plate 7. Two layered plates 8 are provided above the base plate 7. The side walls of the base plate 7 and the layered plates 8 are provided with several side frames 9. The side walls of the base plate 7 and the layered plates 8 are provided with snap-fit ​​seats 10 that engage with the side frames 9.

[0020] The base plate 7 has a rectangular perforation on its surface, and the layered plate 8 has a plurality of insertion holes 11 at equal intervals on its surface for inserting sample tubes. The insertion holes 11 allow for the installation and placement of sample tubes, while the perforations in the base plate 7 increase the flow of warm water at the bottom of the placement mechanism 5.

[0021] All of the aforementioned side frames 9 are U-shaped. The U-shaped side frames 9 can be used to install the base plate 7 and the layered plate 8. In addition, the U-shaped side frames 9 can reduce their own weight and reduce their production cost.

[0022] The placement mechanism 5 also includes a partition frame 12 respectively disposed between the base plate 7 and the layered plates 8, and the partition frame 12 is fixedly connected to the base plate 7 and the layered plates 8 respectively. The partition frame 12 has a plurality of flow channels 13 equidistantly spaced and a plurality of lightweight holes 14 equidistantly spaced. To increase the stability of the sample tubes during placement, the two layered plates 8 can support the sample tubes. Furthermore, to prevent adjacent sample tubes from breaking if one sample tube breaks, the partition frame 12 is provided between the base plate 7 and the layered plates 8, which can hold multiple sample tubes. The separation mechanism 5 is designed to separate multiple independent compartments, allowing for better placement and protection of the sample tubes, thus enhancing their safety during heating. Furthermore, to increase the flow of warm water within the placement mechanism 5, a diversion groove 13 is provided on the diversion rack 12. This, in conjunction with the stirring mechanism 6, increases the flow of warm water within the independent compartments, further improving the heating of the sample tubes. The cooperation between the base plate 7 and the side frame 9 supports the layered plate 8, further enhancing the stability of the placement mechanism 5 during use.

[0023] It should be noted that the lightweight hole 14 can reduce the weight of the placement mechanism 5 itself.

[0024] The bottom of the water bath 4 is provided with several support bars 15 at equal intervals, and the support bars 15 are all wavy. The support bars 15 can support the placement mechanism 5, avoid the placement mechanism 5 from directly contacting the ground of the water bath 4, increase the flow of warm water at the bottom of the placement mechanism 5, further improve the heating speed of the sample tube by the device, and the wavy support bars 15 facilitate the flow of warm water, further improving the practicality of the device.

[0025] The stirring mechanism 6 includes mounting seats 16 symmetrically fixedly installed on both sides of the water bath 4. A rotating rod 17 is provided between the two mounting seats 16, and the two ends of the rotating rod 17 are rotatably connected to the two mounting seats 16 respectively. One end of the rotating rod 17 extends into the device body 1 and is fixedly connected to one end of the synchronous belt structure 19. A drive motor 20 is fixedly installed inside the device body 1. The output end of the drive motor 20 is fixedly connected to the other end of the synchronous belt structure 19. A plurality of stirring blades 18 are fixedly installed at equal intervals on the surface of the rotating rod 17. When the sample tube is placed in the water bath 4 by the placement mechanism 5, the temperature of the warm water around the sample tube will decrease. In order to reduce the fluctuation of the water temperature around the tube, the drive motor 20 drives the rotating rod 17 to rotate through the synchronous belt structure 19. When the rotating rod 17 rotates, it works with the stirring blades 18 to stir the warm water in the water bath 4, mix the warm water in the water bath 4, and thus better heat the sample tube.

[0026] The surface of the stirring blade 18 is provided with a plurality of through holes at equal intervals. The through holes provided on the surface of the stirring blade 18 can reduce the fluctuation of the constant temperature water in the water bath 4 when the stirring blade 18 stirs the constant temperature water, and avoid splashing when the stirring mechanism 6 is stirring, thereby further improving the stability of the device during use.

[0027] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0028] The preferred embodiments of this utility model disclosed above are merely illustrative of the present utility model. These preferred embodiments do not exhaustively describe all details, nor do they limit the utility model to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of this utility model, thereby enabling those skilled in the art to better understand and utilize it. This utility model is limited only by the claims and their full scope and equivalents.

Claims

1. A device integrating cell resuscitation and counting, characterized in that, include: The device body (1) has a control panel (2) fixedly installed at one end for controlling the device and displaying device data. The top of the device body (1) is rotatably connected to a top cover (3). A water bath (4) is opened at the top of the device body (1). Placement mechanisms (5) are placed equidistantly inside the water bath (4). Sample test tubes are inserted into the placement mechanisms (5). A stirring mechanism (6) is installed at the bottom of the water bath (4). The placement mechanism (5) includes a base plate (7), and two layered plates (8) are provided above the base plate (7). The side walls of the base plate (7) and the layered plates (8) are provided with a number of side frames (9), and the side walls of the base plate (7) and the layered plates (8) are provided with snap-fit ​​seats (10) that engage with the side frames (9).

2. The integrated cell resuscitation and counting device according to claim 1, characterized in that, The base plate (7) has a rectangular perforation on its surface, and the layered plate (8) has a plurality of insertion holes (11) at equal intervals on its surface for inserting sample tubes.

3. The integrated cell resuscitation and counting device according to claim 2, characterized in that, Several of the side frames (9) are U-shaped.

4. The integrated cell resuscitation and counting device according to claim 3, characterized in that, The placement mechanism (5) further includes a partition frame (12) respectively disposed between the base plate (7) and the layer plate (8), and the partition frame (12) is fixedly connected to the base plate (7) and the layer plate (8) respectively. A plurality of diversion grooves (13) are equidistantly provided on the partition frame (12), and a plurality of lightweight holes (14) are equidistantly provided on the partition frame (12).

5. The integrated cell resuscitation and counting device according to claim 4, characterized in that, The bottom of the water bath (4) is provided with several support bars (15) at equal intervals, and the support bars (15) are all arranged in a wave shape.

6. The integrated cell resuscitation and counting device according to claim 5, characterized in that, The stirring mechanism (6) includes mounting seats (16) symmetrically fixedly installed on both sides of the water bath tank (4). A rotating rod (17) is provided between the two mounting seats (16), and the two ends of the rotating rod (17) are rotatably connected to the two mounting seats (16) respectively. One end of the rotating rod (17) extends into the device body (1) and is fixedly connected to one end of the synchronous belt structure (19). A drive motor (20) is fixedly installed inside the device body (1). The output end of the drive motor (20) is fixedly connected to the other end of the synchronous belt structure (19). A number of stirring blades (18) are fixedly installed at equal intervals on the surface of the rotating rod (17).

7. The integrated cell resuscitation and counting device according to claim 6, characterized in that, The surface of the stirring blade (18) is provided with several through holes at equal intervals.

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