A disinfection device for rapid batch separation of plant pathogens

Abstract

The utility model relates to the technical field of sterilization device discloses a kind of fast batch separation plant pathogen's sterilization device, including sterilization box, the inside of the sterilization box is placed with several holding devices, the side of the sterilization box is equipped with box, the box is equipped with liquid pump, the output end of the liquid pump is equipped with the spraying device that is placed in the top of sterilization box and cooperates with holding device;The holding device includes plate body, the plate body is evenly equipped with several through holes, the through hole is placed and is equipped with petri dish.The utility model has the advantage compared with prior art in that: multiple plant samples can be operated simultaneously, the plant disinfection speed is fast, and the experimental efficiency is high.

Description

Technical Field

[0001] This utility model relates to the field of disinfection device technology, specifically to a disinfection device for rapid batch separation of plant pathogens. Background Technology

[0002] Plant pathogens are a group of organisms that cause infectious diseases in plants through parasitism. They include five major groups: fungi, prokaryotes, viruses, nematodes, and parasitic plants.

[0003] In various scientific studies, such as the morphology of pathogens, their pathogenicity to host plants, and their genetic variation, pure cultures of pathogens are often required. Therefore, it is necessary to isolate and culture pathogens. Plant pathogen isolation refers to the process of separating pathogens from plant tissues. Before isolation, the tissues must be disinfected. A common disinfection method is to use six petri dishes containing different disinfectant solutions. First, the tissues are disinfected in 75% alcohol for 1-2 minutes, then rinsed in sterile water, then disinfected in 30% sodium hypochlorite for 1-2 minutes, and finally rinsed three times with sterile water.

[0004] Current technology involves using tweezers to transfer tissue blocks in different liquids, and only one plant sample can be disinfected at a time. This method is suitable when there are few plants, but when there are more than 20 plant samples, firstly, different samples need to be disinfected in sequence rather than simultaneously, which is time-consuming. In addition, one sample generally requires 5 tissue blocks, and the transfer of tissue blocks from different samples in different disinfectants also consumes time and affects experimental efficiency; secondly, disinfecting different samples in the same disinfectant increases the probability of cross-contamination, and if the disinfectant is frequently changed to reduce contamination, the experimental time will be increased. Utility Model Content

[0005] (I) Technical problems to be solved The technical problem to be solved by this utility model is to overcome the above difficulties and provide a disinfection device that can operate on multiple plant samples at the same time, has a fast plant sample disinfection speed, and high experimental efficiency for rapid batch isolation of plant pathogens.

[0006] (II) Technical Solution To solve the above-mentioned technical problems, the technical solution provided by this utility model is as follows: a disinfection device for rapid batch separation of plant pathogens, including a disinfection box, a plurality of holding devices placed inside the disinfection box, a box body on one side of the disinfection box, a liquid pump inside the box body, and a spraying device placed on the top of the disinfection box and cooperating with the holding devices at the output end of the liquid pump. The holding device includes a plate with a plurality of through holes evenly distributed on the plate, and a petri dish is placed in each of the through holes.

[0007] As an improvement, the bottom of the disinfection box is inverted conical, and a drain pipe is connected to the bottom of the disinfection box.

[0008] As an improvement, the disinfection box is fixed with symmetrically arranged rods, and the holding device is placed on the rods.

[0009] As an improvement, the plate is provided with a groove that matches the through hole, the culture dish is placed in the groove, and the bottom of the culture dish is provided with a number of water-permeable holes evenly distributed.

[0010] As an improvement, the output end of the liquid pump is connected to a hose, and the other end of the hose is connected to a spraying device.

[0011] As an improvement, the spraying device includes an L-shaped tube connected to a flexible hose, and the L-shaped tube is uniformly provided with a plurality of atomizing nozzles that cooperate with the culture dish.

[0012] As an improvement, the disinfection box is provided with symmetrically arranged placement blocks, and the placement blocks are evenly provided with several notches that cooperate with the L-shaped tubes.

[0013] (III) Beneficial Effects The advantages of this utility model compared with the prior art are as follows: 1. The holding device includes a plate, through holes, and culture dishes. The culture dishes can hold plant samples, and the plate can hold multiple culture dishes, which facilitates personnel to disinfect multiple culture dishes at the same time. 2. The box, liquid pump and spraying device work together. The liquid pump sprays the disinfectant in the box onto the petri dish from the spraying device to disinfect the plant samples in the petri dish. Different liquids are placed in the box to achieve the disinfection of plant samples without moving the petri dish. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the structure of this utility model. Figure 1 .

[0015] Figure 2 This is a schematic diagram of the structure of this utility model. Figure 2 .

[0016] Figure 3 This is a top view of the structure of this utility model.

[0017] Figure 4 This is a utility model Figure 3 A schematic diagram of the structure in cross-section at point AA.

[0018] As shown in the figure: 1. Disinfection box; 2. Container; 3. Box body; 4. Liquid pump; 5. Spray device; 6. Plate; 7. Through hole; 8. Petri dish; 9. Drain pipe; 10. Rod; 11. Groove; 12. Water permeable hole; 13. Flexible hose; 14. L-shaped tube; 15. Atomizing nozzle; 16. Placement block; 17. Notch. Detailed Implementation

[0019] The embodiments of this utility model are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain this utility model, and should not be construed as limiting this utility model.

[0020] Example 1 Combined with appendix Figure 1-4 A disinfection device for rapid batch isolation of plant pathogens includes a disinfection box 1, in which several holding devices 2 are placed. A box body 3 is provided on one side of the disinfection box 1, and a liquid pump 4 is provided in the box body 3. The output end of the liquid pump 4 is provided with a spraying device 5 placed on the top of the disinfection box 1 and cooperating with the holding devices 2.

[0021] The bottom of the disinfection box 1 is inverted cone-shaped, and the bottom of the disinfection box 1 is connected to a drain pipe 9.

[0022] Personnel place multiple plant samples sequentially into the holding device 2, then place the holding device 2 into the disinfection box 1. First, 75% alcohol is placed into the box 3, and the liquid pump 4 is turned on. The liquid pump 4 delivers the 75% alcohol from the box 3 to the spray device 5. The spray device 5 sprays the 75% alcohol onto the plant samples in the holding device 2 for disinfection for 1-2 minutes. Then, personnel sequentially place 30% sodium hypochlorite and sterile water into the box 3, repeating the above steps. The plant samples are disinfected with 30% sodium hypochlorite for 1-2 minutes and rinsed with sterile water for 1-2 minutes. Then, the holding device 2 is removed, and the rinsed liquid falls into the disinfection box 1 and is discharged through the drain pipe 9.

[0023] The disinfection box 1 is fixed with symmetrically arranged rods 10, and the holding device 2 is placed on the rods 10.

[0024] Example 2 Combined with appendix Figure 4 The holding device 2 includes a plate 6, on which a plurality of through holes 7 are evenly provided, and a culture dish 8 is placed in the through holes 7.

[0025] The plate 6 is provided with a groove 11 that matches the through hole 7. The culture dish 8 is placed in the groove 11. The bottom of the culture dish 8 is provided with a plurality of water-permeable holes 12.

[0026] Plant samples are placed in petri dishes 8. The liquid sprayed by the spray device 5 rinses the plant samples in petri dishes 8 and then drains from the water-permeable holes 12 on the petri dishes 8 into the disinfection box 1.

[0027] The output end of the liquid pump 4 is connected to a hose 13, and the other end of the hose 13 is connected to the spraying device 5.

[0028] The spraying device 5 includes an L-shaped tube 14 connected to the hose 13, and the L-shaped tube 14 is uniformly provided with a plurality of atomizing nozzles 15 that cooperate with the culture dish 8.

[0029] The liquid pump 4 in the box 3 transports the liquid in the box 3 through the hose 13 and L-shaped tube 14, and then sprays it from the atomizing nozzle 15 onto the plant sample in the petri dish 8.

[0030] The disinfection box 1 is provided with symmetrically arranged placement blocks 16. The placement blocks 16 are evenly provided with several notches 17 that cooperate with the L-shaped tubes 14. The notches 17 facilitate the placement of the L-shaped tubes 14 by personnel.

[0031] The liquid pump 4 and its matching power supply and controller mentioned in this utility model can be provided by the manufacturer. Apart from that, the circuits, electronic components and modules involved in this utility model are all existing technologies, which can be fully implemented by those skilled in the art, and need not be elaborated.

[0032] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions, alterations, deletions of some features, additions of features, or recombinations of features to the above embodiments within the scope of the present invention without departing from the principles and spirit of the present invention. Any simple modifications, equivalent changes, and alterations made to the above embodiments based on the innovative principles of the present invention shall still fall within the scope of the technical solutions of the present invention.

Claims

1. A disinfection device for rapid batch isolation of plant pathogens, characterized in that: The device includes a disinfection box containing several containers. The box has a body on one side and a liquid pump inside. The output end of the liquid pump has a spray device placed on the top of the disinfection box and used in conjunction with the containers. The holding device includes a plate with a plurality of through holes evenly distributed on the plate, and a petri dish is placed in each of the through holes.

2. The disinfection device for rapid batch isolation of plant pathogens according to claim 1, characterized in that: The bottom of the disinfection box is inverted cone-shaped, and a drain pipe is connected to the bottom of the disinfection box.

3. The disinfection device for rapid batch isolation of plant pathogens according to claim 1, characterized in that: The disinfection box is equipped with symmetrically arranged rods, and the holding device is placed on the rods.

4. The disinfection device for rapid batch isolation of plant pathogens according to claim 1, characterized in that: The plate has grooves that mate with the through holes, and the culture dish is placed in the grooves. The bottom of the culture dish has several water-permeable holes evenly distributed.

5. The disinfection device for rapid batch isolation of plant pathogens according to claim 1, characterized in that: The output end of the liquid pump is connected to a hose, and the other end of the hose is connected to a spraying device.

6. The disinfection device for rapid batch isolation of plant pathogens according to claim 5, characterized in that: The spraying device includes an L-shaped tube connected to a flexible hose, and the L-shaped tube is evenly provided with a plurality of atomizing nozzles that cooperate with the culture dish.

7. The disinfection device for rapid batch isolation of plant pathogens according to claim 6, characterized in that: The disinfection box is provided with symmetrically arranged placement blocks, and the placement blocks are evenly provided with several notches that mate with L-shaped tubes.

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