Drug sensitivity test culture dish with identification zone

Abstract

The utility model discloses a drug sensitive test culture dish with identification area belongs to aquaculture disease detection technical field, including dish bottom and dish cover, in the utility model, vibrio culture and drug sensitive test are integrated in the same culture medium, realize the integration design of culture medium, reduce the operation step of drug sensitive experiment, effectively avoided the pollution of multiple operations to bacterial liquid, promoted the reliability of on -the -spot operation, simultaneously adopts the sealing film to carry out the sealing to antibiotic paper sheet, effectively avoided the situation that antibiotic paper sheet appears to be damp or cross -contamination, realized the anti -pollution function of culture medium, realized the tool -free result reading of culture medium through the concentric circle scale line, realized the quick reading to the diameter of bacteriostatic circle, greatly improved the interpretation efficiency, and adopt turbidimetric module to prepare the bacterial liquid that meets the standard fast, from the source guarantee the accuracy of drug sensitive result, further guarantee the detection accuracy of drug sensitive test.

Description

Technical Field

[0001] This utility model relates to the field of aquaculture disease detection technology, specifically a drug sensitivity test petri dish with a marked area. Background Technology

[0002] Vibrio is a common pathogen in aquaculture, which can easily cause large-scale mortality of shrimp, fish and shellfish. At present, the detection of Vibrio mostly relies on laboratory culture and drug sensitivity testing. In the diagnosis of diseases in aquaculture and other fields, drug sensitivity testing of purified pathogens is a key link to guide precise drug use.

[0003] Currently, most Vibrio cultivation requires laboratory equipment, making it difficult for farmers to conduct on-site testing. This reduces the convenience of drug sensitivity testing. Additionally, the time required for sample delivery makes it difficult for farmers to receive timely guidance on medication, potentially leading to significant losses. Furthermore, traditional culture dishes are easily contaminated in open environments, resulting in inaccurate test results.

[0004] Therefore, this invention provides a culture dish for drug sensitivity testing with a marked area to solve the above problems. Summary of the Invention

[0005] This invention provides a culture dish for drug sensitivity testing with a marked area, aiming to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a drug susceptibility test culture dish with a marking area, comprising a dish bottom and a dish lid, wherein a culture medium is fixedly installed on the upper surface of the dish bottom, and a guide ridge is fixedly installed at the central axis position of the inner wall of the bottom surface of the culture medium, wherein the culture medium is divided into a Vibrio culture area and a drug susceptibility detection area through the guide ridge, the dish lid and the culture medium are mutually compatible, and a key hole is provided on the outer surface of the culture medium; A turbidimetric module is fixedly installed on the upper surface of the dish lid. The turbidimetric module includes a standard area, a pure black area, and a pure white area. The pure black area and the pure white area are distributed adjacent to each other on both sides of the standard area. A latch is fixedly installed on the inner side wall of the dish lid, and the latch and the latch hole are mutually compatible.

[0007] As a preferred technical solution of this application, a plurality of grooves are equidistantly arranged on the inner wall of the bottom surface of the drug sensitivity detection area on the culture medium, and antibiotic paper is placed inside the grooves. A tearable sealing film is provided on the inner wall of the bottom surface of the drug sensitivity detection area, and the sealing film corresponds to the grooves.

[0008] As a preferred technical solution of this application, both the bottom of the dish and the culture medium are made of transparent material. The lower surface of the bottom of the dish is provided with a number of concentric circular scale lines at equal intervals, and the concentric circular scale lines correspond one-to-one with the antibiotic paper strips.

[0009] As a preferred technical solution of this application, a plurality of tool compartments are provided on the inner wall of the top surface of the plate lid, and sterile swabs and pipettes are respectively placed inside the tool compartments. A sealing ring is fixedly installed on the inner side wall of the plate lid.

[0010] As a preferred technical solution of this application, the upper surface of the dish cap is provided with a placement groove, the inner wall of the bottom surface of the placement groove is provided with a plurality of leakage holes, an ampoule is placed inside the placement groove, the ampoule is made of a fragile material, the ampoule is filled with concentrated disinfectant, and the upper surface of the dish cap is provided with a pressing mechanism, the pressing mechanism corresponding to the placement groove.

[0011] As a preferred technical solution of this application, the pressing mechanism includes a sealing plate and a limiting plate. The sealing plate and the placement groove correspond to each other. The sealing plate and the placement groove are fixedly connected by bolts. A through groove is provided at the central axis position of the sealing plate.

[0012] As a preferred technical solution of this application, the limiting plate is located below the sealing plate, and a plurality of breaking rods are fixedly installed at equal intervals on the lower surface of the limiting plate. The lower surface of the breaking rods contacts the upper surface of the ampoule. A pressing column is fixedly installed on the upper surface of the limiting plate, and part of the pressing column extends to the outside of the upper surface of the sealing plate through a through groove.

[0013] As a preferred technical solution of this application, a storage groove is provided on both sides of the pressing column, a connecting spring is fixedly installed on the inner side wall of the storage groove, a right-angled trapezoidal column is fixedly installed on one end of the connecting spring, a part of the right-angled trapezoidal column extends to the outside of the storage groove, and the edge of the right-angled trapezoidal column is in contact with the edge of the storage groove.

[0014] The beneficial effects of this invention are as follows: This invention integrates Vibrio culture and drug susceptibility testing into the same culture medium, achieving an integrated design of the culture medium. This reduces the number of operational steps in the drug susceptibility test, effectively avoiding contamination of the bacterial solution from multiple operations and improving the reliability of on-site operations. Simultaneously, the use of a sealing film to seal the antibiotic discs effectively prevents them from becoming damp or cross-contaminated, achieving the anti-contamination function of the culture medium. The use of concentric circle scale lines enables tool-free reading of the culture medium results, allowing for rapid reading of the diameter of the inhibition zone, greatly improving interpretation efficiency. Furthermore, the use of a turbidimetric module allows for the rapid preparation of standard-compliant bacterial solutions, ensuring the accuracy of drug susceptibility results from the source and further guaranteeing the detection precision of the drug susceptibility test. Attached Figure Description

[0015] Figure 1 This is a three-dimensional structural diagram of the present invention; Figure 2 This is an exploded structural diagram of the present invention; Figure 3 This is a schematic diagram of the decomposed structure of the culture medium in this utility model; Figure 4 This is a three-dimensional structural diagram of the present invention from another angle; Figure 5 This is an exploded structural diagram of the lid of the dish in this utility model; Figure 6 This is an exploded view of the pressing mechanism in this utility model; Figure 7 This is a three-dimensional structural diagram of the lid of the dish in this utility model from another angle.

[0016] In the picture: 1. Dish bottom; 2. Dish lid; 3. Culture medium; 4. Guide ridge; 5. Groove; 6. Antibiotic paper disc; 7. Sealing film; 8. Concentric circle scale line; 9. Pressing mechanism; 901. Sealing plate; 902. Limiting plate; 903. Crushing rod; 904. Pressing column; 905. Storage slot; 906. Connecting spring; 907. Right-angled trapezoidal column; 10. Placement slot; 11. Ampoule; 12. Turbidimetric module; 13. Tool compartment. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0018] This invention provides a culture dish for drug sensitivity testing with a marked area, such as... Figures 1-7 As shown, this drug susceptibility test culture dish with a marked area includes a dish bottom 1 and a dish lid 2. A culture medium 3 is fixedly installed on the upper surface of the dish bottom 1. A guide ridge 4 is fixedly installed at the central axis position of the inner wall of the bottom surface of the culture medium 3. The culture medium 3 is divided into a Vibrio culture area and a drug susceptibility detection area through the guide ridge 4. The dish lid 2 and the culture medium 3 are mutually compatible. A locking hole is provided on the outer surface of the culture medium 3. A turbidimetric module 12 is fixedly installed on the upper surface of the dish lid 2. The turbidimetric module 12 includes a standard area, a pure black area and a pure white area. The pure black area and the pure white area are distributed adjacent to each other on both sides of the standard area. A latch is fixedly installed on the inner side wall of the dish lid 2. The latch and the locking hole are mutually compatible.

[0019] Through the cooperation between the above structures, the culture medium 3 is sealed by the cooperation between the lid 2 and the bottom 1 of the dish. Then, the culture medium 3 is placed in a constant temperature bag for a period of time. After the Vibrio culture is successful, the bacterial solution is collected into a test tube. Then, the test tube containing the bacterial solution is placed behind the turbidimetric module 12. By comparing the blurriness of the bacterial solution and the standard area against the background of pure black area and pure white area, the bacterial solution is calibrated to the standard concentration.

[0020] Several grooves 5 are evenly spaced on the inner wall of the bottom surface of the drug sensitivity detection area on the culture medium 3. Antibiotic paper strips 6 are placed inside the grooves 5. A tearable sealing film 7 is provided on the inner wall of the bottom surface of the drug sensitivity detection area. The sealing film 7 corresponds to the grooves 5.

[0021] Both the bottom of the dish 1 and the culture medium 3 are made of transparent material. Several concentric circular scale lines 8 are equidistantly arranged on the lower surface of the bottom of the dish 1, and the concentric circular scale lines 8 correspond one-to-one with the antibiotic paper strips 6.

[0022] Through the cooperation of the above structures, the sealing film 7 in the drug sensitivity detection area is first torn open to expose the antibiotic paper disc 6. Then, the cultured bacterial solution is dripped into the groove 5 through a pipette. At this time, the bacterial solution and the antibiotic paper disc 6 will react. After the bacterial solution and the antibiotic paper disc 6 have reacted, the bottom 1 of the dish is flipped so that the concentric circle scale line 8 on the bottom 1 faces upward. Then, the diameter of the inhibition zone is observed and read by cooperating with the concentric circle scale line 8. The effective drug is selected according to the size of the inhibition zone produced by the reaction of the bacterial solution and the antibiotic paper disc 6.

[0023] Several tool compartments 13 are provided on the inner wall of the top surface of the plate lid 2. The tool compartments 13 contain sterile swabs and pipettes respectively. A sealing ring is fixedly installed on the inner side wall of the plate lid 2.

[0024] Through the cooperation of the above structures, the water sample is drawn up by the pipette in the tool compartment 13, and then the water sample is dropped into the Vibrio culture area. With the help of the guide ridge 4, the sterile spreading stick is guided to spread the water sample evenly in the Vibrio culture area, so that the water sample is evenly distributed in the Vibrio culture area.

[0025] The upper surface of the dish lid 2 is provided with a placement groove 10. Several leakage holes are provided on the inner wall of the bottom surface of the placement groove 10. An ampoule 11 is placed inside the placement groove 10. The ampoule 11 is made of fragile material and contains concentrated disinfectant. The upper surface of the dish lid 2 is provided with a pressing mechanism 9, which corresponds to the placement groove 10.

[0026] The pressing mechanism 9 includes a sealing plate 901 and a limiting plate 902. The sealing plate 901 corresponds to the placement groove 10. The sealing plate 901 and the placement groove 10 are fixedly connected by bolts. A through groove is provided at the central axis position of the sealing plate 901.

[0027] The limiting plate 902 is located below the sealing plate 901. Several breaking rods 903 are fixedly installed at equal intervals on the lower surface of the limiting plate 902. The lower surface of the breaking rods 903 contacts the upper surface of the ampoule 11. A pressing post 904 is fixedly installed on the upper surface of the limiting plate 902. Part of the pressing post 904 extends to the outside of the upper surface of the sealing plate 901 through a through groove.

[0028] Both sides of the pressing column 904 are provided with a storage groove 905. A connecting spring 906 is fixedly installed on the inner side wall of the storage groove 905. A right-angled trapezoidal column 907 is fixedly installed at one end of the connecting spring 906. Part of the right-angled trapezoidal column 907 extends to the outside of the storage groove 905, and the edge of the right-angled trapezoidal column 907 is in contact with the edge of the storage groove 905.

[0029] Through the cooperation of the above structures, after the experiment is completed, the pressing column 904 is pressed down. At this time, the pressing column 904 will drive the breaking rod 903 to move into the placement groove 10 through the limiting plate 902. Under the action of the breaking rod 903, the ampoule 11 is broken into the placement groove 10. The concentrated disinfectant inside the broken ampoule 11 will flow evenly into the culture medium 3 through the leakage hole. At this time, the concentrated disinfectant will kill the bacteria in the culture medium 3. As the pressing column 904 moves down, it will drive the right-angled trapezoidal column 907 to move down synchronously. Under the action of the inclined plane, the right-angled trapezoidal column 907 will first retract into the storage groove 905. After the right-angled trapezoidal column 907 moves to the lower surface of the sealing plate 901, under the action of the connecting spring 906, the right-angled trapezoidal column 907 will pop out, thereby limiting the pressing column 904 and preventing the pressing column 904 from rebounding. This fixes the fragments of the ampoule 11 in the placement groove 10. At the same time, it is connected to the locking buckle and locking hole in the lid 2, thereby fixing the lid 2 and the bottom 1 of the dish in place and closing it, so that the lid 2 cannot be opened again, ensuring the safety of the culture medium 3 after it is discarded.

[0030] Working principle: Use the pipette in tool compartment 13 to draw water sample, then drop the water sample into the Vibrio culture area. Use a sterile spreader to spread the water sample evenly in the Vibrio culture area. Seal the culture medium 3 by fitting the lid 2 and the bottom 1 of the dish together. Then place the culture medium 3 in a constant temperature bag for a period of time. After the Vibrio culture is successful, collect the bacterial solution. Then place the test tube containing the bacterial solution behind the turbidimetric module 12. Then calibrate the bacterial solution to the standard concentration. Then tear open the sealing film 7 in the drug sensitivity detection area and drop the cultured bacterial solution into the groove 5. After the bacterial solution reacts with the antibiotic paper 6, flip the bottom 1 of the dish so that the bottom 1 faces upward. Read the diameter of the inhibition zone through the concentric circle scale line 8. Then select the effective drug according to the size of the inhibition zone. After the experiment is completed, the pressing mechanism 9 is pressed into the placement slot 10. At this time, the pressing mechanism 9 will break the ampoule 11. The concentrated disinfectant inside the ampoule 11 will flow evenly into the culture medium 3 through the leakage hole to kill the bacteria in the culture medium 3. Under the action of the right-angled trapezoidal column 907, the pressing column 904 is limited so that once the pressing mechanism 9 is pressed, the pressing column 904 cannot rebound, fixing the fragments of the ampoule 11 in the placement slot 10. At the same time, the locking buckle in the dish lid 2 connects the dish lid 2 to the dish bottom 1, so that the dish lid 2 cannot be opened again.

[0031] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A culture dish for drug sensitivity testing with a marked area, comprising a dish bottom (1) and a dish lid (2), characterized in that: The culture medium (3) is fixedly installed on the upper surface of the bottom (1) of the dish. A guide ridge (4) is fixedly installed on the central axis of the inner wall of the bottom surface of the culture medium (3). The culture medium (3) is divided into a Vibrio culture area and an antimicrobial susceptibility detection area through the guide ridge (4). The dish lid (2) and the culture medium (3) are compatible with each other. A lock hole is provided on the outer surface of the culture medium (3). The upper surface of the dish cover (2) is fixedly installed with a turbidity module (12). The turbidity module (12) includes a standard area, a pure black area and a pure white area. The pure black area and the pure white area are distributed adjacent to each other on both sides of the standard area. A latch is fixedly installed on the inner side wall of the dish cover (2). The latch and the lock hole are mutually compatible.

2. The drug susceptibility test culture dish with a marked area according to claim 1, characterized in that: Several grooves (5) are equidistantly arranged on the inner wall of the bottom surface of the drug sensitivity detection area on the culture medium (3). Antibiotic paper (6) is placed inside the groove (5). A tearable sealing film (7) is provided on the inner wall of the bottom surface of the drug sensitivity detection area. The sealing film (7) corresponds to the groove (5).

3. The drug susceptibility test culture dish with a marked area according to claim 1, characterized in that: Both the dish bottom (1) and the culture medium (3) are made of transparent material. Several concentric circular scale lines (8) are equidistantly arranged on the lower surface of the dish bottom (1), and the concentric circular scale lines (8) correspond one-to-one with the antibiotic paper strips (6).

4. The drug susceptibility test culture dish with a marked area according to claim 1, characterized in that: The inner wall of the top surface of the dish cover (2) is provided with several tool compartments (13), and sterile swabs and pipettes are placed inside the tool compartments (13). A sealing ring is fixedly installed on the inner side wall of the dish cover (2).

5. A culture dish with a labeled area for drug sensitivity testing according to claim 1, characterized in that: The upper surface of the lid (2) is provided with a placement groove (10). The inner wall of the bottom surface of the placement groove (10) is provided with several leakage holes. An ampoule (11) is placed inside the placement groove (10). The ampoule (11) is made of fragile material. The ampoule (11) is filled with concentrated disinfectant. The upper surface of the lid (2) is provided with a pressing mechanism (9). The pressing mechanism (9) corresponds to the placement groove (10).

6. A culture dish with a labeling area for drug sensitivity testing according to claim 5, characterized in that: The pressing mechanism (9) includes a sealing plate (901) and a limiting plate (902). The sealing plate (901) corresponds to the placement groove (10). The sealing plate (901) and the placement groove (10) are fixedly connected by bolts. A through groove is provided at the central axis position of the sealing plate (901).

7. A culture dish with a labeled area for drug sensitivity testing according to claim 6, characterized in that: The limiting plate (902) is located below the sealing plate (901). A plurality of breaking rods (903) are fixedly installed at equal intervals on the lower surface of the limiting plate (902). The lower surface of the breaking rods (903) is in contact with the upper surface of the ampoule (11). A pressing column (904) is fixedly installed on the upper surface of the limiting plate (902). Part of the pressing column (904) extends to the outside of the upper surface of the sealing plate (901) through a through groove.

8. A culture dish with a labeled area for drug sensitivity testing according to claim 7, characterized in that: The pressing column (904) has a storage groove (905) on both sides. A connecting spring (906) is fixedly installed on the inner side wall of the storage groove (905). A right-angled trapezoidal column (907) is fixedly installed at one end of the connecting spring (906). Part of the right-angled trapezoidal column (907) extends to the outside of the storage groove (905), and the edge of the right-angled trapezoidal column (907) fits with the edge of the storage groove (905).

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