reagent tube

Abstract

The utility model provides a reagent tube, including main pipe, be provided with the accommodating cavity containing puncture column, one end of accommodating cavity is provided with the sample adding opening, the other end is provided with the liquid outlet, the PCR pipe sets up at the liquid outlet, one of main pipe and PCR pipe is provided with the sealing membrane, makes PCR pipe and main pipe relative insulation, pressing assembly, including pressing top and the side wall part of surrounding in pressing top, the periphery of side wall part and main pipe is detachable sealed connection, the pressing top of pressing assembly is used for sealing sample adding opening, puncture column is used for puncturing sealing membrane under the pressing effect of pressing top, and makes main pipe intercommunication PCR pipe, the side wall part and pressing top integral molding. The utility model reagent tube integral molding setting's pressing assembly, saves assembly cost's time still can be convenient pressing operation of pressing assembly, through the solution transfer of pressing puncture column of pressing assembly, can improve pipetting operation efficiency.

Description

Technical Field

[0001] This utility model belongs to the field of nucleic acid testing consumables technology, and in particular relates to a reagent tube. Background Technology

[0002] Rapid, accurate, sensitive, and quantitative detection of specific nucleic acid sequences is playing an increasingly important role in the diagnosis of human infectious diseases, food security, pathogen identification, global biosecurity, and in environmental analysis, as well as in tracking biocontamination and monitoring environmental quality.

[0003] The existing nucleic acid testing process requires mixing samples and various reagents to obtain the nucleic acid to be tested. During the mixing process, the reagents or samples need to be transferred in various consumable tubes. The liquid transfer process is prone to contamination, resulting in impure nucleic acid and inaccurate test results. Furthermore, the liquid transfer process is relatively complex and cumbersome, which makes the nucleic acid testing efficiency low and the testing time long. Utility Model Content

[0004] The main purpose of this invention is to provide a reagent tube that solves the technical problem of inconvenient operation of existing reagent tubes.

[0005] To achieve the above objectives, this utility model provides a reagent tube, comprising: a main tube having a receiving cavity for accommodating a puncture column, one end of the receiving cavity having a sample dispensing port and the other end having a liquid outlet; a PCR tube disposed at the liquid outlet, wherein one of the main tube and the PCR tube is provided with a sealing membrane to isolate the PCR tube from the main tube; and a pressing assembly including a pressing top and a side enclosure surrounding the pressing top, wherein the side enclosure and the periphery of the main tube are detachably and sealingly connected, the pressing top of the pressing assembly is used to seal the sample dispensing port, and the puncture column is used to puncture the sealing membrane under the pressing action of the pressing top, thereby connecting the main tube to the PCR tube, wherein the side enclosure and the pressing top are integrally formed.

[0006] In this embodiment of the utility model, the pressing assembly includes: a cover, wherein the pressing top and the side portion are both disposed on the cover; a limiting cylinder, which is sealed and detachably connected to the side portion, the limiting cylinder being used to seal with the main pipe, the pressing top being able to drive the limiting cylinder to move relative to the main pipe from an initial position to a final position under pressing action, and at least one of the limiting cylinder and the main pipe being provided with a limiting structure that limits the limiting cylinder to the final position.

[0007] In this embodiment of the utility model, the limiting structure includes a limiting protrusion and a limiting groove. One of the main pipe and the limiting cylinder is provided with the limiting protrusion, and the other is provided with the limiting groove that cooperates with the limiting protrusion.

[0008] In this embodiment of the invention, the limiting structure includes a limiting bend that abuts against the end of the main pipe.

[0009] In this embodiment of the utility model, the side panel and the limiting cylinder are connected by threads, and the limiting cylinder is provided with a limiting ring that abuts against the cover.

[0010] In this embodiment of the invention, the main pipe and the limiting cylinder are sealed together by a sealing ring.

[0011] In this embodiment of the invention, a sealing groove is provided on the outer wall of the main pipe to limit the sealing ring.

[0012] In this embodiment of the utility model, the cover is provided with a pressing protrusion that abuts against the piercing post. The extending direction of the pressing protrusion is consistent with the extending direction of the main tube, and the large flat surface is provided on the pressing protrusion.

[0013] In this embodiment of the invention, the piercing post is provided with a small plane, and the pressing top is provided with a large plane that abuts against the small plane, wherein the cross-sectional dimension of the large plane is larger than that of the small plane.

[0014] In this embodiment of the invention, the reagent tube further includes a protective tube covering the PCR tube, and the protective tube and the main tube are detachably connected.

[0015] Through the above technical solution, the reagent tube provided by this utility model embodiment has the following beneficial effects: The top of the main tube is the sample inlet, and the bottom is the liquid outlet. Swabs, other types of samples, or reagents can be added to the receiving cavity through the sample inlet. When the main tube and the PCR tube are connected, solution can be injected into the PCR tube through the liquid outlet. The PCR tube can be pre-filled with lyophilized reagents or other types of reagents. The pressing top is located at the top of the pressing component, and the size of the pressing top is greater than or equal to the cross-sectional size of the sample inlet. The cross-sectional size of the sample inlet is equal to the maximum inner diameter of the main tube, which provides sufficient pressing surface for the pressing operation of the reagent tube, avoiding the situation where the pressing surface is too small and inconvenient for pressing operation. The process of transferring the solution from the main tube into the PCR tube does not require external pipetting components, and pipetting can be performed while the reagent tube is in a sealed state, which can avoid the contamination of the reagent tube by the external environment during the pipetting process and ensure the accuracy of the solution detection in the reagent tube. Furthermore, the side panel and the pressing top are integrally molded, and the pressing component is movably connected to the main tube. This allows the side panel and the pressing top to move relative to the main tube when the pressing top is pressed, avoiding the inconvenience of pressing in a separate design and saving assembly steps. The reagent tube in this invention, through the cooperation of the main tube, PCR tube, and pressing component, provides a large operating surface for pressing operations. The integrally molded pressing component saves assembly costs and facilitates pressing operations. By pressing the component to puncture the column for solution transfer, the efficiency of pipetting operations is improved.

[0016] Other features and advantages of this invention will be described in detail in the following detailed description section. Attached Figure Description

[0017] The accompanying drawings are provided to illustrate the present invention and form part of the specification. They are used together with the following detailed description to explain the present invention, but do not constitute a limitation thereof. In the drawings: Figure 1 This is a schematic diagram of the reagent tube according to one embodiment of the present invention; Figure 2 This is an exploded structural diagram of a reagent tube according to one embodiment of the present invention; Figure 3 This is a schematic diagram of the cross-sectional structure of the reagent tube according to one embodiment of the present invention; Figure 4 This is a schematic diagram of the reagent tube according to another embodiment of the present invention; Figure 5 This is an exploded structural diagram of the reagent tube according to another embodiment of the present invention; Figure 6 This is a schematic diagram of the cross-sectional structure of the reagent tube according to another embodiment of the present invention; Figure 7 This is a schematic diagram of the reagent tube according to another embodiment of the present invention; Figure 8 This is an exploded structural diagram of the reagent tube according to another embodiment of the present invention; Figure 9 This is a schematic diagram of the cross-sectional structure of the reagent tube according to another embodiment of the present invention.

[0018] Explanation of reference numerals in the attached figures Detailed Implementation

[0019] The specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for illustration and explanation only and are not intended to limit the scope of this utility model.

[0020] The reagent tube according to this utility model is described below with reference to the accompanying drawings.

[0021] like Figures 1 to 9 As shown in the embodiment of this utility model, the reagent tube 100 includes a main tube 1, a PCR tube 3, and a pressing assembly 4. The main tube 1 is provided with a receiving cavity 11 that accommodates the puncturing column 2. One end of the receiving cavity 11 is provided with a sample dispensing port, and the other end is provided with a liquid outlet. The PCR tube 3 is located at the liquid outlet. One of the main tube 1 and the PCR tube 3 is provided with a sealing membrane to isolate the PCR tube 3 from the main tube 1. The pressing assembly 4 includes a pressing top 41 and a side circumference 46 surrounding the pressing top 41. The side circumference 46 and the periphery of the main tube 1 are detachably sealed. The pressing top 41 of the pressing assembly 4 is used to seal the sample dispensing port. The puncturing column 2 is used to puncture the sealing membrane under the pressing action of the pressing top 41 and connect the main tube 1 to the PCR tube 3. The side circumference 46 and the pressing top 41 are integrally formed.

[0022] It should be noted that the reagent tube 100 in this embodiment is mainly used for nucleic acid detection. The main tube 1 is a hollow column with openings at both ends. The PCR tube 3 is connected to the bottom of the main tube 1. The cross-section of the PCR tube 3 can be an inverted cone shape.

[0023] In this embodiment, the top of the main tube 1 is the sample inlet, and the bottom of the main tube 1 is the liquid outlet. Swabs, other types of samples, or reagents can be added to the receiving cavity 11 through the sample inlet. When the main tube 1 and the PCR tube 3 are connected, a solution can be injected into the PCR tube 3 through the liquid outlet. The PCR tube 3 can be pre-filled with lyophilized reagents or other types of reagents. The pressing top 41 is located on top of the pressing component 4, and the size of the pressing top 41 is greater than or equal to the cross-sectional size of the sample inlet. The cross-sectional size of the sample inlet is equal to the maximum inner diameter of the main tube 1, which can provide the maximum pressing surface for the pressing operation of the reagent tube 100, avoiding the situation where the pressing surface is too small and inconvenient for pressing operation. The process of the solution in the main tube 1 entering the PCR tube 3 for pipetting does not require external pipetting components, and pipetting can be performed while the reagent tube 100 is in a sealed state, which can avoid the situation where the reagent tube 100 is contaminated by the external environment during the pipetting process, and can ensure the accuracy of the solution detection in the reagent tube 100. Furthermore, the side panel 46 and the pressing top 41 are integrally formed, and the pressing component 4 and the main tube 1 are movably connected. This allows the side panel 46 and the pressing top 41 to move relative to the main tube 1 when the pressing top 41 is pressed, avoiding the inconvenience of pressing in a separate design and saving assembly steps. In this embodiment, the reagent tube 100, through the cooperation of the main tube 1, the PCR tube 3, and the pressing component 4, provides a large operating surface for pressing operations. The integrally formed pressing component 4 saves assembly costs and facilitates pressing operations. By pressing and piercing the column 2 with the pressing component 4 to transfer the solution, the efficiency of pipetting operations can be improved.

[0024] It should be noted that the pressing assembly 4 includes a cover 42 and a limiting cylinder 45. The piercing post 2 is provided with a small flat surface, and the cover 42 is provided with a large flat surface 43 that abuts against the small flat surface. The cross-sectional dimension of the large flat surface 43 is larger than that of the small flat surface. The pressing top 41 and the side enclosure 46 are both located on the cover 42. The limiting cylinder 45 is sealed and detachably connected to the side enclosure 46. The limiting cylinder 45 is used for a sealed fit with the main pipe 1. Under pressing action, the pressing top 41 can drive the limiting cylinder 45 to move relative to the main pipe 1 from an initial position to a final position. At least one of the limiting cylinder 45 and the main pipe 1 is provided with a limiting structure to limit the limiting cylinder 45 to the final position. Figure 3 , 6 As shown in Figures 9 and 1, the cover 42 is connected to the top of the limiting cylinder 45, and the bottom of the limiting cylinder 45 is sealed to the main pipe 1.

[0025] The top of the cover 42 is provided with a pressing top 41, and the inner side of the pressing top 41 is provided with a large flat surface 43. The large flat surface 43 abuts against the small flat surface of the piercing post 2, which can ensure that the pressing component 4 can always abut against the piercing post 2 during the pressing process, and prevent the pressing component 4 from disengaging from the piercing post 2. In addition, at least one of the limiting cylinder 45 and the main tube 1 is provided with a limiting structure to limit the limiting cylinder 45 in the final position. The limiting structure can limit the limiting cylinder 45 in the final position, which can prevent excessive pressing and ensure the accuracy of each pipetting operation of the reagent tube 100.

[0026] like Figures 4 to 9 As shown, the limiting structure includes a limiting protrusion 12 and a limiting groove 451. One of the main pipe 1 and the limiting cylinder 45 is provided with the limiting protrusion 12, and the other is provided with the limiting groove 451 that cooperates with the limiting protrusion 12. In one embodiment, the outer wall of the main pipe 1 is provided with the limiting protrusion 12, which is annular and surrounds the outer wall of the main pipe 1. The limiting groove 451 is annular and surrounds the inner wall of the limiting cylinder 45. Through the limiting cooperation between the limiting protrusion 12 and the limiting groove 451, the limiting cylinder 45 is prevented from continuing to descend or rising under air pressure after being pressed into place, ensuring that the limiting cylinder 45 is always in the final position after being pressed into place.

[0027] It should be noted that in other embodiments, the limiting structure includes a limiting bend 452 abutting the end of the main tube 1. The limiting bend 452 is located between the pressing top 41 and the limiting groove 451 along the axial direction of the pressing component 4. In this embodiment, the limiting bend 452 is located above the limiting groove 451. The limiting bend 452 in the final position abuts the top plane of the main tube 1, and the limiting protrusion 12 and the limiting groove 451 cooperate to ensure that the pressing component 4 is limited to the final position.

[0028] like Figures 1 to 6 As shown, in one embodiment, the cover 42 and the limiting cylinder 45 are connected by threads, and the limiting cylinder 45 is provided with a limiting ring 453 that abuts against the cover 42. In this embodiment, the cover 42 and the limiting cylinder 45 are connected by threads for sealing. The cover 42 may be provided with internal threads, and the top of the limiting cylinder 45 is provided with external threads that mate with the internal threads, which facilitates the disassembly of the cover 42 and the limiting cylinder 45. When sample addition is required, the sample can be added to the reagent tube 100 by removing the cover 42. When a pressing operation is required, the pressing top 41 on the top of the cover 42 can be pressed, which drives the limiting cylinder 45 to move. The large flat surface 43 on the cover 42 abuts against the small flat surface of the piercing post 2. The limiting bend 452 and the limiting protrusion 12 on the limiting cylinder 45 cooperate with the limiting structure on the main tube 1 to ensure that the pressing component 4 is pressed accurately into place. The limiting ring 453 on the limiting cylinder 45 can abut against the bottom plane of the cover 42 to ensure that the cover 42 is precisely assembled in place.

[0029] like Figures 7 to 9 As shown, in another embodiment, the cover 42 and the limiting cylinder 45 are integrally formed. In this embodiment, the cover 42 and the limiting cylinder 45 are integrally formed, and when samples need to be added, the pressing component 4 can be pulled upward as a whole, which is simple in structure and convenient for the production of pressing components.

[0030] like Figures 1 to 9 As shown, the main tube 1 and the limiting cylinder 45 are sealed together by a sealing ring 5. In this embodiment, the sealing ring 5 can be an O-ring or made of soft rubber. The sealing ring 5 ensures the sealing of the reagent tube 100 during the pressing process of the pressing component 4.

[0031] like Figure 3 and Figure 6 As shown, a sealing groove 13 for a limiting sealing ring 5 is provided on the outer wall of the main pipe 1. In this embodiment, the sealing ring 5 can be confined within the sealing groove 13 of the main pipe 1 to prevent the sealing ring 5 from shifting during the pressing of the pressing component 4.

[0032] In one embodiment, the cover 42 is provided with a pressing protrusion 44 that abuts against the piercing post 2. The extending direction of the pressing protrusion 44 is consistent with the extending direction of the main tube 1, and a large flat surface 43 is provided on the pressing protrusion 44. In this embodiment, the shape of the pressing protrusion 44 can be set according to actual usage requirements, and can be columnar or other shapes. The pressing protrusion 44 extends downward from the top of the cover 42, extends into the limiting cylinder 45 and abuts against the piercing post 2, and has a simple and compact structure.

[0033] like Figures 1 to 9 As shown, the reagent tube 100 also includes a protective tube 6 covering the PCR tube 3, and the protective tube 6 and the main tube 1 are detachably connected. In this embodiment, the side wall of the protective tube 6 may be provided with a light-proof layer. By surrounding the outside of the PCR tube 3 with the protective tube 6, the PCR tube 3 can be physically protected to avoid damage to the PCR tube 3 from other components, and at the same time, the PCR tube 3 can be shielded from light.

[0034] In the description of this utility model, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this utility model, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0035] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between them; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0036] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0037] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A reagent tube, characterized in that, The reagent tube (100) includes: The main tube (1) is provided with a receiving cavity (11) for accommodating the puncturing column (2), and one end of the receiving cavity (11) is provided with a sample inlet and the other end is provided with a liquid outlet; A PCR tube (3) is placed at the outlet. One of the main tube (1) and the PCR tube (3) is provided with a sealing film to isolate the PCR tube (3) from the main tube (1). The pressing assembly (4) includes a pressing top (41) and a side enclosure (46) surrounding the pressing top (41). The side enclosure (46) and the outer periphery of the main tube (1) are detachably sealed. The pressing top (41) of the pressing assembly (4) is used to seal the sample dispensing port. The piercing column (2) is used to pierce the sealing membrane under the pressing action of the pressing top (41) and make the main tube (1) connect to the PCR tube (3). The side enclosure (46) and the pressing top (41) are integrally formed.

2. The reagent tube according to claim 1, characterized in that, The pressing component includes: The cover (42) is provided with the pressing top (41) and the side part (46). The limiting cylinder (45) is sealed and detachably connected to the side enclosure (46). The limiting cylinder (45) is used to seal with the main pipe (1). The pressing top (41) can drive the limiting cylinder (45) to move relative to the main pipe (1) from the initial position to the final position under the pressing action. At least one of the limiting cylinder (45) and the main pipe (1) is provided with a limiting structure to limit the limiting cylinder (45) to the final position.

3. The reagent tube according to claim 2, characterized in that, The limiting structure includes a limiting protrusion (12) and a limiting groove (451). One of the main tube (1) and the limiting cylinder (45) is provided with the limiting protrusion (12), and the other is provided with the limiting groove (451) that is limited and cooperates with the limiting protrusion (12).

4. The reagent tube according to claim 2, characterized in that, The limiting structure includes a limiting bend (452) that abuts against the end of the main tube (1).

5. The reagent tube according to claim 2, characterized in that, The side panel (46) and the limiting cylinder (45) are connected by threads, and the limiting cylinder (45) is provided with a limiting ring (453) that abuts against the cover (42).

6. The reagent tube according to any one of claims 2 to 5, characterized in that, The main tube (1) and the limiting cylinder (45) are sealed together by a sealing ring (5).

7. The reagent tube according to claim 6, characterized in that, The outer wall of the main tube (1) is provided with a sealing groove (13) that limits the sealing ring (5).

8. The reagent tube according to any one of claims 2 to 5, characterized in that, The cover (42) is provided with a pressing protrusion (44) that abuts against the piercing post (2). The extending direction of the pressing protrusion (44) is consistent with the extending direction of the main tube (1), and a large flat surface (43) is provided on the pressing protrusion (44).

9. The reagent tube according to any one of claims 1 to 5, characterized in that, The piercing post (2) is provided with a small plane, and the pressing top (41) is provided with a large plane (43) that abuts against the small plane. The cross-sectional dimension of the large plane (43) is larger than that of the small plane.

10. The reagent tube according to any one of claims 1 to 5, characterized in that, The reagent tube (100) also includes a protective tube (6) covering the PCR tube (3), and the protective tube (6) and the main tube (1) are detachably connected.

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