Methylation dna automatic sampling device

Abstract

The utility model relates to sampling device technical field discloses a kind of methylated DNA automatic sampling device, including equipment platform and sample bottle, the bottom of servo motor is fixedly connected with screw rod, one end of screw rod is threadedly connected with threaded seat, the one end of threaded seat is fixedly connected with connecting seat, the bottom end piston rod of electric push rod is fixedly connected with connecting column, the bottom of connecting column is fixedly connected with rubber plug, the bottom of suction and pressure cylinder is threadedly connected with sampling assembly, the bottom of sampling assembly is connected with sealing sleeve in sleeve joint mode, by starting servo motor, screw rod drives threaded seat and connecting seat to descend, sampling tube is synchronous with connecting seat and descend, make sampling tube insert sample liquid surface below suitable depth, electric push rod is started again, piston rod is driven rubber plug and moves upward by connecting column, under the action of atmospheric pressure, methylated DNA sample in sample bottle is inhaled into sampling tube, when reaching preset sampling amount, electric push rod stops running, and sampling is completed.

Description

Technical Field

[0001] This utility model relates to the field of sampling device technology, specifically to an automated sampling device for methylated DNA. Background Technology

[0002] Methylated DNA is a core object of epigenetics research, and its accurate sampling is a key prerequisite for early tumor diagnosis, disease mechanism research, and drug development. According to industry data, the global methylation detection market is growing at an annual rate of over 25%. The daily processing volume of clinical samples (such as blood, tissues, and body fluids) is large, and the demand for sampling devices that are "micro-precise, free from cross-contamination, and automated and integrated" is becoming increasingly stringent.

[0003] Traditional methylated DNA sampling relies on manual operation, which has problems such as low sampling efficiency and large human error. Manual sampling is achieved by manually controlling the piston stroke, which has poor stroke control precision, resulting in large deviations in the sampling volume. This cannot meet the stringent requirements for sampling volume accuracy in methylation detection. The instability of the sampling volume can interfere with the interpretation of test results, thereby affecting the accuracy of scientific research data or the reliability of clinical diagnosis.

[0004] Therefore, we propose an automated methylated DNA sampling device to solve the above problems. Utility Model Content

[0005] To address the existing technical problems, this utility model provides an automated sampling device for methylated DNA.

[0006] This utility model is achieved using the following technical solution: an automatic sampling device for methylated DNA, comprising a device platform and sample vials. A column is fixedly connected to the top of the device platform, a servo motor is mounted on the top of the column, a lead screw is fixedly connected to the bottom of the servo motor, a threaded seat is threaded to one end of the lead screw, a connecting seat is fixedly connected to one end of the threaded seat, an electric push rod is mounted on the top of one end of the connecting seat, a pressure cylinder is fixedly connected to the bottom of one end of the connecting seat, a connecting column is fixedly connected to the bottom piston rod of the electric push rod, a rubber stopper is fixedly connected to the bottom of the connecting column, the connecting column and the rubber stopper are movably connected inside the pressure cylinder, a sampling component is threadedly connected to the bottom of the pressure cylinder, the sampling component communicates with the inside of the pressure cylinder, and a sealing sleeve is sleeved to the bottom of the sampling component.

[0007] Preferably, the sampling assembly includes a sampling tube and a connector, wherein the sampling tube is fixedly connected to the bottom of the connector, and the connector is threadedly connected to the outside of the bottom end of the pressure cylinder.

[0008] Preferably, the sealing sleeve includes a shell and a sealing plug, the shell being sleeved to the outside of the bottom end of the sampling tube, and the sealing plug being plugged to the inside of the bottom end of the sampling tube.

[0009] Preferably, the connector has a sealing ring inside, and the sealing ring is in contact with the bottom of the suction cylinder.

[0010] Preferably, the column has a movable groove machined inside, and the threaded seat is movably connected inside the movable groove.

[0011] Preferably, two grooves are machined on one side of the column, and two sliders are fixedly connected to one end of the connecting seat, with the sliders movably connected inside the grooves.

[0012] Preferably, the outer shell and the sealing plug are made of rubber material.

[0013] Compared with the prior art, the beneficial effects of this utility model are: In use, this invention involves starting a servo motor, which drives a lead screw to vertically descend along the movable groove of a column. The connecting seat descends synchronously with the threaded seat, and the sampling tube descends synchronously with the connecting seat, allowing the sampling tube to be inserted to a suitable depth below the sample liquid surface. Then, the electric push rod is activated, causing its piston rod to retract upwards. This retraction drives the rubber stopper to move upwards within the suction cylinder via the connecting column. A negative pressure is created between the suction cylinder and the sampling assembly. Under atmospheric pressure, the methylated DNA sample in the sample vial is drawn into the sampling tube. When the preset sampling volume is reached, the electric push rod stops, and the sampling is complete. This allows for sampling at different heights of the sample vial and control of the sampling volume. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is a schematic diagram of the connection structure of the connector of this utility model; Figure 3 This is a schematic diagram of the connection structure between the sampling component and the pressure cylinder of this utility model.

[0015] In the diagram: 1. Equipment platform; 2. Sample bottle; 3. Column; 4. Sampling assembly; 401. Sampling tube; 402. Connector; 5. Sealing sleeve; 501. Outer shell; 502. Sealing plug; 6. Servo motor; 7. Lead screw; 8. Threaded seat; 9. Connecting seat; 10. Electric push rod; 11. Pressure cylinder; 12. Connecting column; 13. Rubber stopper; 14. Sealing ring; 15. Movable groove; 16. Slider; 17. Slide groove. Detailed Implementation

[0016] The present invention will be further described below with reference to the accompanying drawings and specific embodiments. It should be noted that, without conflict, the various embodiments or technical features described below can be arbitrarily combined to form new embodiments.

[0017] Example 1: Please refer to Figure 1 - Figure 3 An automated methylated DNA sampling device according to this embodiment includes a device platform 1 and a sample vial 2. A column 3 is fixedly connected to the top of the device platform 1. A servo motor 6 is installed on the top of the column 3. A lead screw 7 is fixedly connected to the bottom of the servo motor 6. A threaded seat 8 is threadedly connected to one end of the lead screw 7. A connecting seat 9 is fixedly connected to one end of the threaded seat 8. An electric push rod 10 is installed on the top of one end of the connecting seat 9. A pressure cylinder 11 is fixedly connected to the bottom of one end of the connecting seat 9. A connecting column 12 is fixedly connected to the piston rod at the bottom of the electric push rod 10. A rubber stopper 13 is fixedly connected to the bottom of the connecting column 12. The connecting column 12 and the rubber stopper 13 are movably connected inside the pressure cylinder 11. A sampling component 4 is threadedly connected to the bottom of the pressure cylinder 11. The sampling component 4 is connected to the inside of the pressure cylinder 11. A sealing sleeve 5 is sleeved to the bottom of the sampling component 4. Servo motor 6 provides a high-precision power source for vertical movement. It can achieve precise control of speed and rotation angle through pulse signals, converting the rotational motion of servo motor 6 into the vertical linear motion of threaded seat 8, so that sampling component 4 can move up and down. The electric push rod 10 is a high-precision miniature electric push rod, which can accurately control the extension and retraction stroke of the piston rod. It is rigidly connected to the connecting column 12 to provide stable suction power for sampling. The suction cylinder 11 is made of transparent polytetrafluoroethylene material, which makes it easy to observe the internal sampling status. Its inner wall is precision polished to reduce friction with the rubber stopper 13 and ensure sealing performance. The rubber stopper 13 is made of highly elastic and corrosion-resistant rubber. Its outer diameter is precisely matched with the inner diameter of the suction cylinder 11 to achieve an interference fit, ensuring no air leakage during the suction process. When the electric push rod 10 drives the connecting column 12 to move the rubber stopper 13 upward, a negative pressure is formed inside the suction cylinder 11. The methylated DNA sample in the sample bottle 2 is drawn into the sampling component 4 by atmospheric pressure. The up and down movement of the rubber stopper 13 can be precisely controlled by the electric push rod 10, thereby achieving precise adjustment of the sampling amount and meeting the detection requirement of "sampling amount deviation ≤ 2%". Furthermore, the sampling assembly 4 includes a sampling tube 401 and a connector 402. The sampling tube 401 is fixedly connected to the bottom of the connector 402. The connector 402 is threadedly connected to the outside of the bottom end of the pressure cylinder 11. A sealing ring 14 is provided inside the connector 402, and the sealing ring 14 is in contact with the bottom of the pressure cylinder 11. The threaded connection between the connector 402 and the suction cylinder 11 facilitates the disassembly and replacement of the sampling component 4, and allows for the replacement of the appropriate sampling tube 401 for different specifications of sample bottles 2 or different sampling requirements. The sealing ring 14 is made of food-grade silicone material, which has good elasticity and sealing performance. When the connector 402 is threadedly locked with the suction cylinder 11, the sealing ring 14 is squeezed and deformed to fill the gap between the connector 402 and the suction cylinder 11, preventing air from entering the suction cylinder 11 under negative pressure, and ensuring the sealing performance and the accuracy of the sampling amount during the sampling process. Furthermore, the sealing sleeve 5 includes a housing 501 and a sealing plug 502. The housing 501 is sleeved to the outside of the bottom end of the sampling tube 401, and the sealing plug 502 is plugged to the inside of the bottom end of the sampling tube 401. The housing 501 and the sealing plug 502 are made of rubber material. The sealing sleeve 5 is made of elastic rubber material. Its design purpose is to double seal the bottom of the sampling tube 401 after sampling to prevent sample leakage, volatilization or external contamination. The outer shell 501 is sleeved on the outside of the sampling tube 401 and fixed in position by elastic tension. The sealing plug 502 is inserted into the internal channel of the sampling tube 401 to directly block the sample outflow path. The double sealing structure further improves the sealing reliability. Furthermore, the column 3 has a movable groove 15 machined inside, and the threaded seat 8 is movably connected inside the movable groove 15. The movable groove 15 provides vertical movement space for the threaded seat 8, and its inner wall is tightly fitted with the side wall of the threaded seat 8, restricting the rotational freedom of the threaded seat 8 and ensuring that it moves only in the vertical direction. Furthermore, two grooves 17 are machined on one side of the column 3, and two sliders 16 are fixedly connected to one end of the connecting seat 9. The sliders 16 are movably connected inside the grooves 17. The grooves 17 and the sliders 16 form a guiding mechanism, which further improves the movement stability of the connecting seat 9, avoids the connecting seat 9 from tilting due to the radial force of the lead screw 7, and ensures that the sampling tube 401 always remains vertical, preventing contamination caused by touching the inner wall or bottom of the sample bottle 2.

[0018] Working principle: First, place the sample vial 2 containing the methylated DNA sample in the designated position on the equipment stage 1, ensuring that the mouth of the sample vial 2 is aligned with the vertical projection of the sampling tube 401; at this time, the servo motor 6 and the electric push rod 10 are in the initial state, the rubber stopper 13 is located at the bottom of the suction cylinder 11, the sampling tube 401 is in a high position, and the sealing sleeve 5 is not installed on the sampling tube 401. Then, the servo motor 6 is started, which drives the lead screw 7 to rotate. The lead screw 7 drives the threaded seat 8 to descend vertically along the movable groove 15 of the column 3. The connecting seat 9 descends synchronously with the threaded seat 8, and the slider 16 slides along the slide groove 17 to ensure a smooth descent. The sampling tube 401 descends synchronously with the connecting seat 9. According to the liquid level in the sample bottle 2, the descent distance is precisely controlled by the servo motor 6 to insert the sampling tube 401 into the appropriate depth below the sample liquid level. Restart the electric push rod 10. The piston rod of the electric push rod 10 retracts upward, driving the rubber stopper 13 to move upward in the suction cylinder 11 through the connecting column 12. The suction cylinder 11 and the sampling assembly 4 form a negative pressure. Under the action of atmospheric pressure, the methylated DNA sample in the sample bottle 2 is sucked into the sampling tube 401. According to the preset sampling volume, the electric push rod 10 precisely controls the upward stroke of the rubber stopper 13. When the preset sampling volume is reached, the electric push rod 10 stops operating, and the sampling is completed. Finally, after closing the electric push rod 10, manually put the sealing sleeve 5 on the bottom of the sampling tube 401, so that the sealing plug 502 is inserted into the internal channel of the sampling tube 401, and the outer shell 501 wraps around the bottom of the sampling tube 401 to achieve double sealing; then rotate the connector 402 of the sampling assembly 4 to remove the sampling assembly 4 from the bottom of the suction cylinder 11, and the sampled sample can be transferred to the detection equipment. The operation is simple and convenient.

[0019] The above embodiments are merely preferred embodiments of this utility model and should not be construed as limiting the scope of protection of this utility model. Any non-substantial changes and substitutions made by those skilled in the art based on this utility model shall fall within the scope of protection claimed by this utility model.

Claims

1. An automated sampling device for methylated DNA, comprising a device stage (1) and sample vials (2), characterized in that, The top of the equipment platform (1) is fixedly connected to a column (3), a servo motor (6) is installed on the top of the column (3), a lead screw (7) is fixedly connected to the bottom of the servo motor (6), a threaded seat (8) is threaded to one end of the lead screw (7), a connecting seat (9) is fixedly connected to one end of the threaded seat (8), an electric push rod (10) is installed on the top of one end of the connecting seat (9), and a pressure cylinder (11) is fixedly connected to the bottom of one end of the connecting seat (9). The bottom piston rod of the electric push rod (10) is fixedly connected to a connecting column (12), and the bottom of the connecting column (12) is fixedly connected to a rubber plug (13). The connecting column (12) and the rubber plug (13) are movably connected inside the pressure cylinder (11). The bottom of the pressure cylinder (11) is threadedly connected to a sampling component (4), which is connected to the inside of the pressure cylinder (11). The bottom of the sampling component (4) is sleeved with a sealing sleeve (5).

2. The automated methylated DNA sampling device according to claim 1, characterized in that, The sampling assembly (4) includes a sampling tube (401) and a connector (402). The sampling tube (401) is fixedly connected to the bottom of the connector (402), and the connector (402) is threadedly connected to the outside of the bottom end of the pressure cylinder (11).

3. The automated methylated DNA sampling device according to claim 2, characterized in that, The sealing sleeve (5) includes a shell (501) and a sealing plug (502). The shell (501) is sleeved to the outside of the bottom end of the sampling tube (401), and the sealing plug (502) is plugged to the inside of the bottom end of the sampling tube (401).

4. The automated methylated DNA sampling device according to claim 2, characterized in that, The connector (402) is provided with a sealing ring (14) inside, and the sealing ring (14) is in contact with the bottom of the suction cylinder (11).

5. The automated methylated DNA sampling device according to claim 1, characterized in that, The column (3) has a movable groove (15) machined inside, and the threaded seat (8) is movably connected inside the movable groove (15).

6. The automated methylated DNA sampling device according to claim 1, characterized in that, Two grooves (17) are machined on one side of the column (3), and two sliders (16) are fixedly connected to one end of the connecting seat (9). The sliders (16) are movably connected inside the grooves (17).

7. The automated methylated DNA sampling device according to claim 3, characterized in that, The outer shell (501) and the sealing plug (502) are made of rubber material.

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