A sampling device for microbial diversity studies

Abstract

The utility model discloses a sampling device of microorganism diversity research, include: equipment frame, the top of equipment frame is installed with equipment box, and the bottom of equipment frame is installed with mobile subassembly, sampling angle adjusting mechanism, sampling angle adjusting mechanism includes mounting seat and four sets of angle adjusting subassembly, and four sets of angle adjusting subassembly are installed in the bottom of equipment box with equal interval, and the hinge connection between angle adjusting subassembly and mounting seat, sampling bucket, sampling bucket is detachably connected in the bottom of mounting seat, sample push -out subassembly, sample push -out subassembly rotation is connected in the middle position of equipment box bottom, and the drive subassembly is installed in equipment box, and the output of push -out subassembly is installed with push rod, and the push plate is slidably connected in sampling bucket, and the push plate is connected with push rod between push plate and push plate, control system, the utility model solves the limitation that only vertical sampling of traditional device can, improves the comprehensiveness and pertinence of microorganism diversity research sample, avoids the sample deviation caused by single angle.

Description

Technical Field

[0001] This utility model relates to the field of microbial sampling equipment technology, and in particular to a sampling device for microbial diversity research. Background Technology

[0002] Microbial diversity research is a core research direction in life sciences, environmental sciences, and ecology. By analyzing the species composition, abundance distribution, and functional characteristics of microorganisms in specific habitats, it provides important theoretical support for maintaining ecological balance, controlling environmental pollution, optimizing agricultural production, and developing biomedicine. As the first step in microbial diversity research, the sampling process directly determines the accuracy and reliability of subsequent experimental results such as DNA extraction, high-throughput sequencing, and community analysis. Therefore, it places stringent requirements on the scientific rigor and practicality of sampling devices.

[0003] Existing microbial sampling devices still have many limitations in practical applications: First, the flexibility of sampling angle adjustment is insufficient. Traditional devices are mostly fixed vertical sampling structures, while the distribution of microorganisms in natural habitats is often affected by factors such as terrain (e.g., slopes, gullies), medium characteristics (e.g., clayey soil, flowing water), and vegetation cover. A single sampling angle is difficult to obtain representative samples from different micro-habitats, which can easily lead to sample bias and affect the comprehensiveness of diversity analysis. Second, contamination or loss is prone to occur during sample collection and transfer. Traditional sampling buckets are mostly obtained by directly pouring or using tweezers, which is not only cumbersome but may also introduce external microbial contamination. At the same time, sample residue on the inner wall of the sampling bucket can lead to insufficient actual sample volume for analysis, especially for rare microbial groups, where residual loss may cause loss of species information. Third, the mobility and adaptability of the devices are poor. Some sampling devices lack convenient moving components, making it difficult to adapt to the operational needs of complex terrain in the field. Moreover, the connection between the sampling bucket and the device is mostly a fixed structure, which makes it inconvenient to change sampling buckets of different specifications according to sampling depth and medium type, thus limiting the applicability of the devices.

[0004] Furthermore, with the rapid development of microbiome technologies, research demands for standardized and efficient sampling are increasing. The shortcomings of existing devices in areas such as precise angle control, non-destructive sample recovery, and automated operation have become key factors restricting research efficiency and the reliability of results. Therefore, developing a microbial diversity research sampling device with functions such as flexible angle adjustment, non-destructive sample extraction, convenient movement and replacement, and automated control has significant practical implications and application value. Utility Model Content

[0005] The purpose of this invention is to provide a sampling device for microbial diversity research, in order to solve the problems existing in the prior art.

[0006] To achieve the above objectives, this utility model provides the following solution: This utility model provides a sampling device for microbial diversity research, comprising: An equipment rack, with an equipment box mounted on top and a movable component mounted on the bottom; A sampling angle adjustment mechanism, comprising a mounting base and four sets of angle adjustment components, wherein the four sets of angle adjustment components are circumferentially and equally spaced at the bottom of the equipment box, and the angle adjustment components are hinged to the mounting base; A sampling container, which is detachably connected to the bottom of the mounting base; A sample ejection assembly is rotatably connected to the bottom center of the equipment box. A drive assembly is installed inside the equipment box. The drive assembly and the sample ejection assembly are driven by a universal coupling. A push rod is installed at the output end of the ejection assembly. The push rod passes through the mounting base, the top of the sampling barrel, and extends into the sampling barrel. A push plate is slidably connected inside the sampling barrel. The push plate and the push rod are threadedly connected. A control system is used to control the operation of the overall device.

[0007] According to the sampling device for microbial diversity research provided by this utility model, the angle adjustment component includes an angle adjustment motor, a fixed base is fixedly connected to the bottom of the equipment box, the angle adjustment motor is fixedly connected to the fixed base, a rocker arm is rotatably connected to the fixed base, a rocker rod is rotatably connected to the end of the rocker arm, and the other end of the rocker rod is rotatably connected to the mounting base.

[0008] According to the sampling device for microbial diversity research provided by this utility model, the sample ejection component includes an electrically controlled telescopic rod, the top of which is connected to the output shaft of the drive component via a universal coupling, and the output end of which is coaxially and fixedly connected to the push rod.

[0009] According to the sampling device for microbial diversity research provided by this utility model, the driving component includes a drive motor and a transmission. The drive motor and the transmission are both installed in the equipment box. The output shaft of the drive motor is connected to the input shaft of the transmission, and the output shaft of the transmission is axially connected to the universal coupling.

[0010] According to the sampling device for microbial diversity research provided by this utility model, a number of sets of screws are fixedly connected to the top of the sampling bucket, and a flange is fixedly connected to the bottom of the mounting base. The screws pass through the flange and are fixed by nuts.

[0011] According to the sampling device for microbial diversity research provided by this utility model, a pressure sensor is installed on the push rod, and the pressure sensor is connected to the control system.

[0012] The present invention discloses the following technical effects: The device utilizes four sets of circumferentially spaced angle adjustment components that are hinged to the mounting base, allowing for flexible adjustment of the sampling angle according to different habitats such as slopes, gullies, and flowing water bodies. The universal coupling ensures stable power transmission when the angle changes, overcoming the limitation of traditional devices that can only sample vertically. This enhances the comprehensiveness and specificity of samples for microbial diversity research and avoids sample deviation caused by a single angle.

[0013] The device is equipped with mobile components for easy transport in complex terrains in the field. The detachable sampling bucket design makes it easy to replace with different sizes to adapt to different sampling depths. The control system realizes automated control of angle adjustment and sample ejection, reducing manual intervention, simplifying the operation process, shortening the sampling cycle, and the operating status of each component is controllable in real time, ensuring that the sampling process is standardized and repeatable, and providing support for large-scale microbial diversity surveys. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0015] Figure 1 This is a schematic diagram of the sampling device for microbial diversity research according to this utility model. Figure I ; Figure 2 This is a schematic diagram of the sampling device for microbial diversity research according to this utility model. Figure II ; Figure 3 for Figure 2 Enlarged view of point A in the middle.

[0016] The components include: 1. Equipment rack; 2. Equipment box; 3. Mounting base; 4. Sampling bucket; 5. Universal coupling; 6. Angle adjustment motor; 7. Fixed base; 8. Rocker arm; 9. Rocker stick; 10. Electrically controlled telescopic rod; and 11. Screw. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0018] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the utility model will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0019] Reference Figures 1-3 This utility model provides a sampling device for microbial diversity research, comprising: Equipment rack 1, equipment box 2 is installed on the top of equipment rack 1, and a movable component is installed at the bottom of equipment rack 1; The sampling angle adjustment mechanism includes a mounting base 3 and four sets of angle adjustment components. The four sets of angle adjustment components are installed at equal intervals around the bottom of the equipment box 2, and the angle adjustment components are hinged to the mounting base 3. Sampling bucket 4 is detachably connected to the bottom of mounting base 3; The sample ejection assembly is rotatably connected to the middle of the bottom of the equipment box 2. The equipment box 2 is equipped with a drive assembly. The drive assembly and the sample ejection assembly are driven by a universal coupling 5. The output end of the ejection assembly is equipped with a push rod. The push rod passes through the mounting base 3, the top of the sampling barrel 4 and extends into the sampling barrel 4. A push plate is slidably connected inside the sampling barrel 4. The push plate and the push rod are threadedly connected. The control system is used to control the operation of the overall device.

[0020] After the device is transported to the target sampling area via the movable component at the bottom of the equipment frame 1, the control system activates four sets of circumferentially spaced angle adjustment components according to the needs of different sampling habitats such as soil slopes, shallow water bodies, and sediment surfaces. Each set of components adjusts the tilt angle of the mounting base 3 through coordinated telescopic hinge action, ensuring that the detachable sampling bucket 4 at the bottom is precisely aligned with the sampling point, ensuring that the sampling angle meets the research requirements. Subsequently, the control system drives the drive component in the equipment box 2, which, through the universal coupling 5, drives the sample ejection component to operate. At this time, the push rod drives the push plate to move upward along the inner wall of the sampling bucket 4 to reserve sampling space. Then, through the auxiliary mechanism, the sampling bucket 4 is inserted into the target medium to complete sample collection. After sampling, the drive component is activated in reverse. The universal coupling 5 adapts to the angle change of the mounting base 3 to ensure stable power transmission. The push rod pushes the push plate downward at a uniform speed, pushing the sample in the bucket completely out to the collection container. The entire process does not require disassembling the sampling bucket 4. The control system monitors the operation of each component in real time to ensure that the sampling and ejection process is accurate and controllable, completing the collection of microbial samples.

[0021] Further optimization of the scheme: the angle adjustment component includes an angle adjustment motor 6, a fixed base 7 is fixedly connected to the bottom of the equipment box 2, the angle adjustment motor 6 is fixedly connected to the fixed base 7, a rocker arm 8 is rotatably connected to the fixed base 7, a rocker rod 9 is rotatably connected to the end of the rocker arm 8, and the other end of the rocker rod 9 is rotatably connected to the mounting base 3.

[0022] After the device is moved to the target area via the bottom moving assembly of the equipment rack 1, the control system initiates the angle adjustment process based on the sampling habitat requirements: the angle adjustment motors 6 of the four angle adjustment components operate synchronously, driving the rocker arm 8 on the bottom fixed seat 7 of the equipment box 2 to rotate. The rocker arm 8 pushes and pulls the mounting seat 3 through the end rocker rod 9. The four components work together to achieve precise adjustment of the tilt angle of the mounting seat 3, and the sampling bucket 4, which is fixed by the flange and screw 11, is aligned with the sampling point. Subsequently, the drive motor of the drive component is reduced in speed and increased in torque by the gearbox, and the power is transmitted to the electrically controlled telescopic rod 10 of the sample ejection component through the universal coupling 5. The electrically controlled telescopic rod 10 drives the push rod (the pressure sensor monitors the thrust data in real time), so that the push plate moves upward along the inner wall of the sampling bucket 4 to reserve the sampling space, and then the sampling bucket 4 is inserted into the target medium to complete the collection. After sampling, the drive motor reverses its rotation, and the angle of the universal coupling 5 and the mounting base 3 changes to ensure stable power. The electric telescopic rod 10 pushes the push plate downward at a constant speed, and the pressure sensor provides feedback data to the control system to adjust the thrust, ensuring that the sample is pushed out completely. There is no need to disassemble the sampling bucket 4 throughout the process. The operating status of each component is monitored in real time by the control system, realizing full automation and precision of the sampling process.

[0023] Further optimization of the design resulted in a sample ejection assembly including an electrically controlled telescopic rod 10. The top of the electrically controlled telescopic rod 10 is connected to the output shaft of the drive assembly via a universal coupling 5, and the output end of the electrically controlled telescopic rod 10 is coaxially and fixedly connected to the push rod.

[0024] The scheme is further optimized. The drive components include a drive motor and a gearbox. Both the drive motor and the gearbox are installed in the equipment box 2. The output shaft of the drive motor is connected to the input shaft of the gearbox, and the output shaft of the gearbox is axially connected to the universal coupling 5.

[0025] To further optimize the design, several sets of screws 11 are fixedly connected to the top of the sampling bucket 4, and a flange is fixedly connected to the bottom of the mounting base 3. The screws 11 pass through the flange and are fixed by nuts.

[0026] The design was further optimized by installing a pressure sensor on the push rod, which is connected to the control system.

[0027] An angle sensor is installed at the mounting base 3 or the angle adjustment component to form a closed-loop control with the control system, providing real-time feedback of the tilt angle data of the mounting base 3, thus avoiding angle deviation when the four sets of angle adjustment motors 6 operate in tandem; at the same time, the motor control algorithm is optimized and the angle fine-tuning function is added to achieve millimeter-level angle calibration for complex habitats such as steep slopes and narrow crevices, further ensuring that the sampling angle is highly consistent with the research needs.

[0028] A wear-resistant elastic sealing gasket is added to the edge of the push plate, which not only enhances the fit between the push plate and the inner wall of the sampling bucket 4 to avoid sample residue, but also buffers the impact force when pushing out to prevent damage to fragile samples (such as microbial aggregates in sediments). At the same time, a graded speed adjustment module is added to the electrically controlled telescopic rod 10. Combined with pressure sensor data, the push plate running speed is adjusted according to the medium characteristics such as soil viscosity and water sample flowability to adapt to the pushing requirements of different types of samples.

[0029] The equipment box 2, angle adjustment motor 6, drive motor and other key components are waterproofed and dustproofed. The inner wall of the sampling bucket 4 is sprayed with a low-friction and non-stick coating to reduce sample adsorption. A fault early warning module is added to the control system. Combined with the data of pressure sensor and angle sensor, the machine will automatically stop and alarm when abnormal thrust or angle adjustment jamming occurs. At the same time, the shock absorption structure of the moving components is optimized to improve the stability of the device in the transportation of rugged terrain and adapt to complex field sampling environment.

[0030] In the description of this utility model, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0031] The embodiments described above are merely preferred embodiments of the present utility model and are not intended to limit the scope of the present utility model. Various modifications and improvements made to the technical solutions of the present utility model by those skilled in the art without departing from the spirit of the present utility model should fall within the protection scope defined by the claims of the present utility model.

Claims

1. A sampling device for microbial diversity research, characterized in that, include: Equipment rack (1), with equipment box (2) installed on the top of the equipment rack (1) and a moving component installed on the bottom of the equipment rack (1); The sampling angle adjustment mechanism includes a mounting base (3) and four sets of angle adjustment components. The four sets of angle adjustment components are installed at equal intervals around the bottom of the equipment box (2), and the angle adjustment components are hinged to the mounting base (3). Sampling bucket (4), which is detachably connected to the bottom of the mounting base (3); The sample ejection assembly is rotatably connected to the bottom center of the equipment box (2). A drive assembly is installed inside the equipment box (2). The drive assembly and the sample ejection assembly are driven by a universal coupling (5). A push rod is installed at the output end of the ejection assembly. The push rod passes through the mounting base (3), the top of the sampling barrel (4), and extends into the sampling barrel (4). A push plate is slidably connected inside the sampling barrel (4). The push plate and the push rod are threadedly connected. A control system is used to control the operation of the overall device.

2. The sampling device for microbial diversity research according to claim 1, characterized in that, The angle adjustment assembly includes an angle adjustment motor (6), a fixed base (7) is fixedly connected to the bottom of the equipment box (2), the angle adjustment motor (6) is fixedly connected to the fixed base (7), a rocker arm (8) is rotatably connected to the fixed base (7), a rocker arm (9) is rotatably connected to the end of the rocker arm (8), and the other end of the rocker arm (9) is rotatably connected to the mounting base (3).

3. The sampling device for microbial diversity research according to claim 1, characterized in that, The sample ejection assembly includes an electrically controlled telescopic rod (10), the top of which is connected to the output shaft of the drive assembly via a universal coupling (5), and the output end of which is coaxially and fixedly connected to the push rod.

4. A sampling device for microbial diversity research according to claim 1, characterized in that, The drive assembly includes a drive motor and a transmission. Both the drive motor and the transmission are installed in the equipment box (2). The output shaft of the drive motor is connected to the input shaft of the transmission, and the output shaft of the transmission is axially connected to the universal coupling (5).

5. A sampling device for microbial diversity research according to claim 1, characterized in that, The top of the sampling bucket (4) is fixedly connected with several sets of screws (11), and the bottom of the mounting base (3) is fixedly connected with a flange. The screws (11) pass through the flange and are fixed by nuts.

6. A sampling device for microbial diversity research according to claim 1, characterized in that, A pressure sensor is installed on the push rod, and the pressure sensor is connected to the control system.

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