A device for rapid visual detection of constant temperature nucleic acid amplification

Abstract

The utility model discloses a device for constant temperature nucleic acid amplification rapid visualization detection, including connecting shell, install the shaking device in connecting shell, the top of connecting shell is equipped with heating device, the shaking device includes support shell, support shell fixedly connected in connecting shell, install drive motor in support shell, drive motor's output installs connecting rod, connecting rod rotatoryly connected in support shell, install connecting shaft in connecting rod, the outside rotatoryly connected of connecting shaft has the shaking shell of uniform speed rotation, the frame body for limiting when the shaking shell rotates is rotatoryly connected through the pivot outside the shaking shell, the fixedly connected of drive board outside the shaking shell, drive board outside fixedly connected has movable plate, install the elastic clamping plate with the fixed test tube outside movable plate, in the utility model, realize the mixing effect of more sufficient, even of test tube internal reaction system, improve amplification stability and amplification efficiency.

Description

Technical Field

[0001] This practical field of molecular diagnostic technology specifically relates to a device for rapid and visual detection of isothermal nucleic acid amplification. Background Technology

[0002] Isothermal nucleic acid amplification visualization detection technology is widely used in the field of rapid on-site nucleic acid testing. Its detection process usually requires amplification of the reaction system in the test tube under isothermal conditions to achieve rapid amplification of nucleic acids. However, in existing isothermal nucleic acid amplification detection equipment, the test tube is usually in a static state during the amplification process. Problems such as uneven local concentration of the reaction solution, polymer adhesion to the walls, and insufficient mixing of the amplification system are prone to occur inside the test tube, which affects the amplification speed and the stability of the detection results. In addition, most existing equipment only has simple heating functions in terms of isothermal control, and the temperature feedback and display of the reaction chamber are not timely enough. Temperature fluctuations are prone to occur during long-term amplification, resulting in reduced amplification efficiency or even false negatives. Utility Model Content

[0003] Therefore, this invention provides a device for rapid and visual detection of isothermal nucleic acid amplification, in order to solve the problems in existing isothermal nucleic acid amplification detection equipment, such as the test tube being in a static state during the amplification process, and the reaction solution being prone to local uneven concentration, polymer sticking to the wall, and insufficient mixing of the amplification system.

[0004] To achieve the above objectives, this utility model provides the following technical solution:

[0005] A device for rapid visual detection of isothermal nucleic acid amplification includes a connecting shell, a shaking device installed inside the connecting shell, and a heating device installed above the connecting shell.

[0006] The shaking device includes a support shell, which is fixedly connected inside a connecting shell. A drive motor is installed inside the support shell, and a connecting rod is installed at the output end of the drive motor. The connecting rod is rotatably connected inside the support shell, and a connecting shaft is installed inside the connecting rod. A shaking shell that rotates at a uniform speed is rotatably connected outside the connecting shaft. A frame for limiting the rotation of the shaking shell is rotatably connected outside the shaking shell via a rotating shaft. A drive plate is fixedly connected outside the shaking shell, and a movable plate is fixedly connected outside the drive plate. An elastic clamping plate for fixing test tubes is installed outside the movable plate.

[0007] Furthermore, the frame is rotatably connected to the support shell via a pivot.

[0008] Furthermore, an observation window is installed on the front of the connecting shell, and the observation window is made of acrylic sheet.

[0009] Furthermore: the heating device includes a top plate, and a sealing gasket is installed under the top plate, the sealing gasket being secured inside the connecting shell.

[0010] Furthermore, a heating rod is installed on the lower surface of the top plate, and a temperature sensor is installed on the lower surface of the top plate.

[0011] Furthermore, a temperature gauge is mounted above the temperature sensor, and the temperature sensor and the temperature gauge are electrically connected.

[0012] This utility model has the following advantages:

[0013] 1. In this utility model, an elastic clamping plate is set outside the movable plate to fix the test tube, so that the test tube can be reliably clamped on the movable plate. In use, the drive motor inside the connecting shell is started. The output end of the drive motor drives the connecting rod to rotate. While the connecting rod rotates, it drives the connecting shaft installed inside to rotate synchronously. This further causes the shaking shell outside the connecting shaft to rotate. During the rotation, the shaking shell forms a stable rotation in the support shell with the help of the external rotating shaft. At the same time, it achieves uniform rotation between the frames through the rotating shaft, thereby driving the drive plate and the movable plate fixed outside the shaking shell to perform uniform shaking motion synchronously. Since the elastic clamping plate fixes the test tube, the uniform shaking of the movable plate will directly act on the test tube, so that the reaction liquid inside the test tube will be continuously mixed at a uniform speed during the amplification process. This effectively avoids the reaction system from layering, local uneven concentration, and reactants adhering to the test tube wall, etc., and achieves a more thorough and uniform mixing effect of the reaction system inside the test tube, thereby improving the amplification stability and amplification efficiency.

[0014] 2. In this utility model, by setting a heating device above the connecting shell and installing a heating rod on the lower surface of the top plate, the internal space of the connecting shell can be continuously and stably heated by the heating rod during use, ensuring the constant temperature working environment required for nucleic acid amplification. The temperature inside the connecting shell is monitored in real time by a temperature sensor installed on the lower surface of the top plate. The temperature sensor can instantly sense temperature changes and transmit the signal to the temperature gauge located above it. The temperature gauge displays the current cavity temperature intuitively. The combined effect of heating by the heating rod, temperature feedback by the temperature sensor, and real-time temperature display by the temperature gauge allows the user to monitor the temperature status inside the connecting shell at any time, thereby facilitating constant temperature treatment of the reaction system, avoiding amplification instability caused by temperature fluctuations, and improving the accuracy and reliability of nucleic acid amplification detection.

[0015] Other features and advantages of this utility will be set forth in the following description and will be apparent in part from the description or learned by practicing this utility. Attached Figure Description

[0016] To more intuitively illustrate the prior art and this application, exemplary drawings are provided below. It should be understood that the specific shapes and structures shown in the drawings should not generally be regarded as limiting conditions for implementing this application; for example, based on the technical concept disclosed in this application and the exemplary drawings, those skilled in the art are able to easily make conventional adjustments or further optimizations to the addition / reduction / classification, specific shapes, positional relationships, connection methods, size ratios, etc. of certain units (components).

[0017] Figure 1 This is a schematic diagram of a device for rapid visual detection of isothermal nucleic acid amplification provided in an embodiment of this application.

[0018] Figure 2 This is a schematic diagram of a device for rapid visual detection of isothermal nucleic acid amplification according to this invention.

[0019] Figure 3 This is a schematic diagram of a device for rapid visual detection of isothermal nucleic acid amplification according to this invention.

[0020] Figure 4 This is a schematic diagram of a device for rapid visual detection of isothermal nucleic acid amplification according to this invention.

[0021] 1. Connecting shell; 2. Observation window; 3. Shaking device; 301. Support shell; 302. Drive motor; 303. Connecting rod; 304. Connecting shaft; 305. Shaking shell; 306. Frame; 307. Drive plate; 308. Movable plate; 309. Elastic clamping plate; 4. Heating device; 401. Top plate; 402. Sealing gasket; 403. Heating rod; 404. Temperature sensor; 405. Thermometer. Detailed Implementation

[0022] The following specific embodiments illustrate the implementation of this utility model. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. It should be understood that these embodiments are merely for further explanation of this utility model and should not be construed as limiting the scope of protection of this utility model. Technical engineers in the field can make some non-essential improvements and adjustments to this utility model based on the above-described invention. All other embodiments obtained by those skilled in the art based on the embodiments in this utility model without creative effort are within the scope of protection of this utility model.

[0023] Please see Figures 1-4 ,

[0024] This utility model provides a device for rapid visual detection of isothermal nucleic acid amplification: it includes a connecting shell 1, a shaking device 3 installed inside the connecting shell 1, and a heating device 4 installed above the connecting shell 1;

[0025] The shaking device 3 includes a support shell 301, which is fixedly connected to the connecting shell 1. A drive motor 302 is installed inside the support shell 301. A connecting rod 303 is installed at the output end of the drive motor 302. The connecting rod 303 is rotatably connected inside the support shell 301. A connecting shaft 304 is installed inside the connecting rod 303. A shaking shell 305 that rotates at a constant speed is rotatably connected to the outside of the connecting shaft 304. A frame 306 for limiting the rotation of the shaking shell 305 is rotatably connected to the outside of the shaking shell 305 via a rotating shaft. A drive plate 307 is fixedly connected to the outside of the shaking shell 305. A movable plate 308 is fixedly connected to the outside of the drive plate 307. An elastic clamping plate 309 for fixing the test tube is installed outside the movable plate 308.

[0026] Specifically, such as Figures 2-3 As shown, the frame 306 is rotatably connected to the support shell 301 via a pivot. An observation window 2 is installed on the front of the connecting shell 1, and the observation window 2 is made of acrylic sheet.

[0027] Specifically, such as Figure 4 As shown, the heating device 4 includes a top plate 401, a sealing gasket 402 is installed under the top plate 401, the sealing gasket 402 is snapped into the connecting shell 1, a heating rod 403 is installed on the lower surface of the top plate 401, a temperature sensor 404 is installed on the lower surface of the top plate 401, a thermometer 405 is installed above the temperature sensor 404, and an electrical connection is formed between the temperature sensor 404 and the thermometer 405.

[0028] Working principle and usage: An elastic clamping plate 309 is installed outside the movable plate 308 to secure the test tube, allowing the test tube to be reliably secured on the movable plate 308. During use, the drive motor 302 inside the connecting shell 1 is activated. The output of the drive motor 302 drives the connecting rod 303 to rotate. Simultaneously, the connecting rod 303 drives the connecting shaft 304 installed inside it to rotate synchronously, further causing the shaking shell 305 outside the connecting shaft 304 to rotate as well. During rotation, the shaking shell 305 achieves stable rotation within the support shell 301 via an external rotating shaft, while simultaneously achieving uniform rotation between the frames 306 via the rotating shaft. This causes the drive plate 307 and the movable plate 308, fixed to the outside of the shaking shell 305, to perform a synchronous, uniform shaking motion. Because the elastic plate 309 fixes the test tube, the uniform shaking of the movable plate 308 will directly act on the test tube, so that the reaction solution inside the test tube will be continuously and uniformly mixed during the amplification process. By setting a heating device 4 above the connecting shell 1 and installing a heating rod 403 on the lower surface of the top plate 401, the internal space of the connecting shell 1 can be continuously and stably heated by the heating rod 403 during use, ensuring the constant temperature working environment required for nucleic acid amplification. The temperature inside the connecting shell 1 is monitored in real time by a temperature sensor 404 installed on the lower surface of the top plate 401. The temperature sensor 404 can instantly sense temperature changes and transmit the signal to the temperature gauge 405 located above it, and the temperature gauge 405 can intuitively display the current cavity temperature.

[0029] The above description is only a preferred embodiment of this utility model and is not intended to limit this utility model. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A device for rapid and visual detection of isothermal nucleic acid amplification, characterized in that, Includes a connecting shell (1), a shaking device (3) is installed inside the connecting shell (1), and a heating device (4) is installed above the connecting shell (1); The shaking device (3) includes a support shell (301), which is fixedly connected to the connecting shell (1). A drive motor (302) is installed inside the support shell (301). A connecting rod (303) is installed at the output end of the drive motor (302). The connecting rod (303) is rotatably connected inside the support shell (301). A connecting shaft (304) is installed inside the connecting rod (303). A shaking shell (305) that rotates at a constant speed is rotatably connected outside the connecting shaft (304). A frame (306) for limiting the rotation of the shaking shell (305) is rotatably connected outside the shaking shell (305) via a rotating shaft. A drive plate (307) is fixedly connected outside the shaking shell (305). A movable plate (308) is fixedly connected outside the drive plate (307). An elastic clamping plate (309) for fixing the test tube is installed outside the movable plate (308).

2. The device for rapid visual detection of isothermal nucleic acid amplification according to claim 1, characterized in that, The frame (306) is rotatably connected to the support shell (301) via a pivot.

3. The device for rapid visual detection of isothermal nucleic acid amplification according to claim 1, characterized in that, The front of the connecting shell (1) is equipped with an observation window (2), and the observation window (2) is made of acrylic sheet.

4. The device for rapid visual detection of isothermal nucleic acid amplification according to claim 1, characterized in that, The heating device (4) includes a top plate (401), and a sealing gasket (402) is installed under the top plate (401). The sealing gasket (402) is locked inside the connecting shell (1).

5. The device for rapid visual detection of isothermal nucleic acid amplification according to claim 4, characterized in that, A heating rod (403) is installed on the lower surface of the top plate (401), and a temperature sensor (404) is installed on the lower surface of the top plate (401).

6. The device for rapid visual detection of isothermal nucleic acid amplification according to claim 5, characterized in that, A thermometer (405) is mounted above the temperature sensor (404), and the temperature sensor (404) and the thermometer (405) are electrically connected.

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