A cell constant voltage test system

Abstract

The utility model provides a cell constant voltage test system, include: constant voltage chamber, is used for placing petri dish, constant temperature heating device, is evenly arranged in the outer wall of constant voltage chamber, is used for providing preset constant temperature for constant voltage chamber, external air source or vacuum pump, is communicated with constant voltage chamber through pipeline, is used for providing constant positive pressure or constant negative pressure to constant voltage chamber, pressure -relief valve, with constant voltage chamber intercommunication, is used for maintaining preset pressure in constant voltage chamber, temperature sensor is used for gathering temperature value in constant voltage chamber, pressure sensor is used for gathering pressure value in constant voltage chamber, controller, with constant temperature heating device, external air source or vacuum pump, pressure -relief valve, temperature sensor and pressure sensor electric connection respectively, is used for adjusting the working condition of constant temperature heating device, external air source or vacuum pump and pressure -relief valve according to temperature value and pressure value, to keep the temperature and pressure constant in constant voltage chamber, the utility model discloses can provide constant temperature and constant positive / negative pressure stress in vitro, and it is convenient to carry out relevant research.

Description

Technical Field

[0001] This utility model relates to the field of cell testing technology, and in particular to a cell constant pressure testing system. Background Technology

[0002] Cell culture technology is relatively mature under normal pressure conditions and can reflect cell growth status well. However, research on the influencing factors during cell culture by applying compressive stress under constant positive or negative pressure is still insufficient. Existing methods or devices are difficult to observe pathological changes in cells caused by compressive stress under constant pressure throughout the process.

[0003] Currently, commonly used pressurization methods mainly include the pressure plate contact method and the fluid pressure transfer method. The former directly applies pressure to the surface of tissues or cells using a pressure plate, while the latter utilizes the static pressure of gases or liquids to achieve pressurization. Both of these methods apply pressure under environmental conditions and cannot simultaneously meet the key conditions required for in vitro cell culture—constant temperature (approximately 37.0℃), saturated humidity (95%±3%), suitable CO2 concentration (approximately 5%), and the ability to automatically apply and maintain constant positive / negative pressure stress.

[0004] While a few studies have used devices such as gas-injection culture flasks to investigate the effects of different pressures or pressurization times on cells, or to explore the effects of continuous pressure on osteocytes, no devices have been reported that can simulate a constant positive / negative pressure environment in vivo and automatically apply corresponding compressive stress. The complex structure of biological tissues and organs, as well as individual differences, result in a diverse mechanical environment in somatic cells, increasing the difficulty of related research.

[0005] In vivo, tissue cells experience pressure loads, a common phenomenon. For example, bones, as load-bearing structures, participate in force transmission and protect internal organs; articular cartilage withstands pressure transmitted through bones to maintain growth and metabolism; and intraocular pressure maintains the shape and function of the eyeball and can cause pathological changes when abnormal. Establishing a cell culture and testing system that can simulate a constant positive / negative pressure stress environment in vivo is of great significance for studying the mechanical properties of cells under pressure loads.

[0006] Therefore, it is necessary to develop a cell constant pressure testing system that can provide constant temperature and constant positive / negative compressive stress in vitro to more realistically simulate the compressive stress environment of cells in vivo, thus facilitating related research. Utility Model Content

[0007] The purpose of this invention is to provide a cell constant pressure testing system, which aims to solve the technical problem that existing cell culture devices cannot provide a constant temperature and automatically apply constant positive / negative pressure stress.

[0008] To achieve the above objectives, this utility model provides a cell constant pressure testing system, comprising:

[0009] A constant-pressure chamber is used to hold a culture dish.

[0010] A constant temperature heating device is evenly arranged on the outer wall of the constant pressure chamber to provide a preset constant temperature inside the constant pressure chamber;

[0011] An external gas source or vacuum pump is connected to the constant pressure chamber via a pipeline to provide a constant positive / negative pressure to the constant pressure chamber;

[0012] A pressure relief valve, connected to the constant pressure chamber, is used to maintain a preset pressure within the constant pressure chamber;

[0013] Temperature sensor, used to collect temperature values ​​inside the constant pressure chamber;

[0014] Pressure sensors are used to collect pressure values ​​within a constant pressure chamber;

[0015] The controller is electrically connected to the constant temperature heating device, the external air source or vacuum pump, the pressure relief valve, the temperature sensor, and the pressure sensor, respectively, and is used to adjust the working state of the constant temperature heating device, the external air source or vacuum pump, and the pressure relief valve according to the temperature and pressure values, so as to maintain the constant temperature and pressure in the constant pressure chamber.

[0016] As a further improvement to the above solution, the cell constant pressure testing system also includes a heat insulation layer, which surrounds the constant temperature heating device.

[0017] As a further improvement to the above solution, the cell constant pressure testing system also includes a housing, the constant pressure chamber, the constant temperature heating device, and the heat insulation layer are all disposed inside the housing, and the heat insulation layer is placed between the constant temperature heating device and the inner wall of the housing.

[0018] As a further improvement to the above solution, the housing is also provided with a control panel, and the control panel is communicatively connected to the controller;

[0019] The control panel includes a control unit for setting preset temperature and preset pressure, and a display unit for displaying the temperature and pressure values ​​in the constant pressure chamber in real time.

[0020] As a further improvement to the above solution, the constant pressure chamber is a container with an opening at the top, and the opening is provided with a pressure-resistant cover, which can be removed from the opening through a quick-sealing locking structure.

[0021] As a further improvement to the above solution, the quick-sealing and locking structure includes at least one set of snap-fit ​​mechanisms or threaded tightening mechanisms distributed circumferentially along the opening of the constant pressure chamber, and works with a sealing ring to achieve quick disassembly and reliable sealing of the pressure-resistant cover and the opening, so as to ensure airtightness under pressure.

[0022] As a further improvement to the above solution, the gas provided by the external gas source is a mixed gas containing a predetermined concentration of carbon dioxide to meet the gas environment requirements for cell culture.

[0023] As a further improvement to the above solution, the controller is configured to receive the detection signals from the temperature sensor and the pressure sensor, and control the constant temperature heating device, the external air source or vacuum pump and the pressure relief valve according to the set parameters to achieve closed-loop regulation of the temperature and pressure in the constant pressure chamber.

[0024] As a further improvement to the above solution, the constant temperature heating device is a flexible or rigid electric heating element arranged around the outer wall of the constant pressure cavity. It has a heating element arranged inside and a heat-conducting structure configured to transfer heat evenly in the circumference and bottom of the constant pressure cavity, so as to achieve uniform distribution and stable control of the temperature inside the cavity.

[0025] Because this utility model adopts the above technical solutions, the beneficial effects of this application are as follows:

[0026] This invention provides a cell constant pressure testing system. By setting up a constant pressure chamber, a constant temperature heating device, an external gas source or vacuum pump, a pressure relief valve, and temperature and pressure sensors and a controller, the system can simultaneously achieve precise control of temperature and pressure within the same device. This solves the technical problem that existing cell culture devices are difficult to automatically load and maintain constant positive / negative pressure stress in a constant temperature, humidity and CO2 environment.

[0027] Specifically, the constant-temperature heating device is evenly distributed on the outer wall of the constant-pressure chamber, forming a closed-loop temperature control circuit with the temperature sensor and controller. This maintains the temperature within the chamber within the suitable range for cell culture (e.g., 37.0℃), preventing temperature fluctuations from affecting cell state. Under the unified control of the controller, the external gas source or vacuum pump and pressure relief valve can adjust the air intake or extraction rate in real time based on pressure sensor feedback, thereby establishing and maintaining the set positive or negative pressure within the constant-pressure chamber to meet the requirements of simulating in vivo compressive stress environments. The linked control of temperature and pressure parameters ensures stable operation of the system during long-term testing, reducing human intervention and improving experimental repeatability. Furthermore, real-time acquisition and feedback control of pressure and temperature data enable the system to accurately reproduce the required mechanical environment for different experimental schemes, facilitating a more realistic simulation of in vivo tissue compressive stress conditions in vitro. This provides a reliable experimental platform for studying the mechanical properties of cells under pressure loads. This system achieves integrated control of key environmental factors in cell culture while ensuring airtightness and ease of operation, improving the accuracy and efficiency of testing. Attached Figure Description

[0028] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0029] Figure 1 This is a schematic diagram of a cell constant pressure testing system disclosed in this utility model;

[0030] Figure 2 This is a partial cross-sectional schematic diagram of the constant pressure chamber covered with a pressure-resistant cover disclosed in this utility model.

[0031] Figure label:

[0032] 1. Constant pressure chamber; 2. Constant temperature heating device; 3. External gas source; 4. Pressure relief valve; 5. Insulation layer; 6. Shell; 7. Control panel; 8. Pressure resistant cover; 9. Vacuum pump; 10. Petri dish.

[0033] The purpose, features, and advantages of this utility model will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0034] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0035] It should be noted that all directional indicators (such as up, down, etc.) in the embodiments of this utility model are only used to explain the relative positional relationship and movement of each component in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicator will also change accordingly.

[0036] Furthermore, in this utility model, descriptions involving "first," "second," etc., are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features.

[0037] Furthermore, the technical solutions of the various embodiments of this utility model can be combined with each other, but only if they are based on the ability of a person skilled in the art to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such combination of technical solutions does not exist and is not within the scope of protection claimed by this utility model.

[0038] See Figure 1 and Figure 2 This invention provides a cell constant pressure testing system, including a constant pressure chamber 1, a constant temperature heating device 2, an external gas source 3 or a vacuum pump 9, a pressure relief valve 4, a temperature sensor, a pressure sensor, and a controller. The constant pressure chamber 1 is a container with an open top for holding standard-sized culture dishes 10. Its opening is equipped with a pressure-resistant cap 8, which is circumferentially fixed to the opening by a quick-sealing locking structure, maintaining good airtightness under pressurized or negative pressure conditions, thereby preventing pressure fluctuations caused by gas leakage.

[0039] The constant-temperature heating device 2 is evenly distributed on the outer wall of the constant-pressure chamber 1. It preferably uses electric heating elements and is equipped with a heat-conducting structure to ensure that heat is evenly transferred along the circumference of the chamber. In conjunction with the controller and temperature sensor, the temperature inside the chamber is stably maintained at a suitable value for cell culture (e.g., 37.0℃). Uniform heating combined with closed-loop temperature control can effectively reduce the temperature difference inside the chamber, ensure that the cells are in a stable thermal environment, and avoid the impact of temperature unevenness or drift on experimental results.

[0040] An external gas source 3 or a vacuum pump 9 is connected to the constant pressure chamber 1 via a pipeline. The external gas source 3 can provide a mixed gas containing a predetermined concentration of carbon dioxide to meet the gas environment requirements for cell culture; the vacuum pump 9 can extract gas from the chamber to establish a negative pressure. A pressure relief valve 4 is also connected to the constant pressure chamber 1 and is used to release excess gas or replenish gas after reaching the set pressure to maintain the preset positive or negative pressure within the chamber; preferably, a control valve for controlling the on / off state is also provided on the connecting pipeline, for details see [link to details]. Figure 1 The external gas source 3 is connected to the inner cavity of the constant pressure chamber 1 through a pipeline and a control valve. A three-way connector is also provided on the corresponding pipeline, which is connected to the vacuum pump 9. When only a mixed gas of a predetermined concentration of carbon dioxide needs to be introduced, the control valve is opened and the vacuum pump 9 is closed. When only a negative pressure needs to be established in the constant pressure chamber 1, the control valve is closed and the vacuum pump 9 is turned on to evacuate the constant pressure chamber 1.

[0041] The pressure sensor collects the pressure value inside the cavity in real time and transmits it to the controller. The controller compares the value with the preset pressure value and automatically adjusts the start / stop and flow rate of the external air source 3 or vacuum pump 9, as well as the opening degree of the pressure relief valve 4, to achieve closed-loop stable pressure control.

[0042] In practical use, the culture dish 10 inoculated with the cells to be tested is placed into the constant pressure chamber 1 and covered with the pressure-resistant cap 8, which is then locked in place by a quick-sealing locking structure. The controller activates the constant temperature heating device 2 according to a preset program, raising the temperature inside the chamber to and maintaining it at the target value. Simultaneously, the external gas source 3 is activated to fill the chamber with a carbon dioxide mixture, or the vacuum pump 9 is activated to evacuate the chamber. The pressure sensor feeds back real-time pressure data to the controller, which then adjusts the air intake, evacuation, or depressurization actions accordingly to stabilize the pressure inside the chamber at the required positive or negative pressure value. Because both temperature and pressure can be controlled independently and synchronously, the system can maintain a constant temperature and humidity environment and mechanical loading conditions during long-term testing, thus realistically simulating the compressive stress environment of tissues in vivo.

[0043] After maintaining constant pressure (positive / negative pressure) for a certain period of time, open the pressure-resistant cover 8 and take out the culture dish 10. Then you can observe the structural changes and physiological responses of the cells under the set pressure conditions and obtain the mechanical property data of the cells under pressure load.

[0044] In this invention, the constant pressure chamber 1, pressure-resistant cover 8, and quick-sealing locking structure ensure reliable sealing under pressure; the constant temperature heating device 2, in conjunction with a temperature sensor and controller, achieves uniform and stable temperature control, meeting the temperature control requirements for cell growth; the external gas source 3 or vacuum pump 9, in conjunction with a pressure sensor, pressure relief valve 4, and controller, enables the automatic establishment and maintenance of positive / negative pressure. These features effectively address the shortcomings of existing technologies that cannot simultaneously meet the requirements of constant temperature and humidity and automatic application of constant positive / negative pressure stress, thereby improving the accuracy and repeatability of in vitro cell mechanical behavior testing.

[0045] As a preferred embodiment, in order to further reduce heat loss and improve the stability of temperature control, the cell constant pressure testing system of this embodiment is also provided with a heat insulation layer 5 and a shell 6.

[0046] The insulation layer 5 surrounds the outside of the constant-temperature heating device 2. Its material can be low in thermal conductivity to reduce heat loss from the cavity to the external environment. Since the constant-temperature heating device 2 is evenly distributed on the outer wall of the constant-pressure cavity 1, the insulation layer 5 concentrates more of the generated heat within the cavity, thereby improving heating efficiency and reducing energy consumption. During closed-loop temperature control, this structure reduces temperature fluctuations within the cavity caused by changes in ambient temperature, maintaining a more stable temperature near the set value, which is beneficial for cells to maintain a stable growth environment during long-term constant-pressure testing.

[0047] The shell 6 serves as an external protective structure. The constant pressure chamber 1, the constant temperature heating device 2, and the insulation layer 5 are all housed within the shell 6, with the insulation layer 5 positioned between the constant temperature heating device 2 and the inner wall of the shell 6. The shell 6 not only provides mechanical protection, preventing external collisions or contamination from entering the constant pressure chamber 1 area, but also, together with the insulation layer 5, forms a double thermal insulation boundary, further limiting the lateral diffusion of heat and environmental interference. The enclosed structure of the shell 6 also helps maintain the relative stability of humidity and gas composition within the chamber, and, in conjunction with the external gas path, reduces pressure disturbances caused by changes in environmental airflow.

[0048] In practical use, after the culture dish 10 is placed and sealed, the controller activates the constant temperature heating device 2. Heat is evenly transferred to the inner wall of the constant pressure chamber 1 through the heat-conducting structure. The insulation layer 5 inhibits heat conduction to the outer wall, and the shell 6 blocks the direct influence of the external ambient temperature, thus forming a stable temperature field. During pressure control, the combination of the shell 6 and the insulation layer 5 effectively isolates changes in external temperature and airflow. The pressure value measured by the pressure sensor more accurately reflects the set pressure state inside the chamber, reducing pressure drift caused by environmental interference.

[0049] The aforementioned insulation layer 5 and shell 6 work synergistically with the constant pressure chamber 1, constant temperature heating device 2, sensor and controller to solve the problems of temperature being easily affected by environmental interference, high energy consumption and insufficient pressure stability of existing devices during long-term operation, thus enabling the system to have higher reliability and experimental repeatability when simulating constant positive / negative compressive stress environment in the body.

[0050] In a preferred embodiment, to facilitate the setting and monitoring of experimental parameters by operators, the cell constant pressure testing system is further provided with a control panel 7 on the housing 6. The control panel 7 is communicatively connected to the controller. The control panel 7 includes a control unit and a display unit: the control unit allows the operator to input or select preset temperature and preset pressure values; the display unit can display in real time the temperature and pressure values ​​inside the constant pressure chamber 1 collected by the temperature and pressure sensors and processed by the controller.

[0051] In practice, the control unit can use buttons, knobs, or a touch interface to input the target temperature (e.g., 37.0℃) and target pressure (positive or negative) according to the experimental plan. After input, the controller drives the constant temperature heating device 2, the external gas source 3 or vacuum pump 9, and the pressure relief valve 4 according to the set parameters, and achieves closed-loop control through feedback from temperature and pressure sensors. The display unit can be configured with a digital or graphical interface to synchronously display the real-time temperature and pressure values ​​inside the chamber, allowing the operator to intuitively grasp the system's operating status during the experiment, promptly identify abnormalities, and make adjustments.

[0052] This configuration integrates parameter setting and status monitoring functions onto the surface of the housing 6, allowing for basic operations and data reading without the need for external monitoring equipment, thus simplifying experimental preparation and process management. The control unit and display unit communicate directly with the controller, avoiding information transmission delays or errors. This ensures that the set temperature and pressure are quickly and accurately converted into control commands, and guarantees that the displayed data matches the actual working conditions inside the chamber. In conjunction with the aforementioned constant-temperature heating device 2, insulation layer 5, and housing 6, the system maintains stable temperature and pressure while improving operational convenience and experimental controllability. This reduces experimental deviations caused by inadequate manual monitoring or incorrect parameter input, further enhancing the reliability and repeatability of the in vitro simulated constant-pressure stress environment.

[0053] As a preferred embodiment, in order to balance ease of operation and sealing reliability under pressure, the constant pressure chamber 1 is designed as a container with an opening at the top, and a pressure-resistant cover 8 is provided at the opening. The pressure-resistant cover 8 is detachably fixed to the opening by a quick-sealing locking structure.

[0054] In practice, the opening shape of the constant pressure chamber 1 matches the placement path of the standard culture dish 10, facilitating the secure placement of the culture dish 10 into the chamber. The pressure-resistant cap 8 is made of a transparent or opaque material with good pressure resistance, allowing it to withstand set positive or negative pressure without deformation or leakage while maintaining convenient observation. The quick-sealing locking structure includes at least one set of snap-fit ​​mechanisms or threaded tightening mechanisms evenly distributed along the circumference of the opening, and a sealing ring is provided at the mating surface between the cap and the chamber opening. The snap-fit ​​mechanism can be locked and unlocked simply by pressing or flicking, making installation and removal quick; the threaded tightening mechanism can achieve uniform compression through rotation, suitable for applications requiring higher sealing reliability. The sealing ring is made of an elastic, corrosion-resistant material, which undergoes uniform compression deformation under locking force, filling the microscopic gaps at the mating surface and forming a continuous sealed band.

[0055] This design ensures stable pressure during pressurization and depressurization by preventing gas leakage or infiltration within the chamber through the locking structure and sealing ring. It also allows for rapid opening and closing of the chamber before and after experiments, shortening sample replacement time and reducing environmental disturbances caused by frequent opening and closing. When working in conjunction with the aforementioned constant-temperature heating device 2, insulation layer 5, and shell 6, it enables efficient replacement of the culture dish 10 and system reuse while maintaining constant temperature and pressure. Due to its reliable sealing performance, the system can operate stably for extended periods, avoiding pressure drift or temperature and humidity changes caused by insufficient airtightness, thereby improving the accuracy and reproducibility of cell culture and testing results under constant-pressure conditions.

[0056] As a preferred embodiment, in order to achieve high-precision and continuous stable control of temperature and pressure in constant pressure chamber 1, the controller of this embodiment is configured to receive detection signals output by temperature sensor and pressure sensor, and coordinate the control of constant temperature heating device 2, external gas source 3 or vacuum pump 9 and pressure relief valve 4 according to preset temperature and pressure parameters, thereby completing the closed-loop regulation of the environment and pressure inside the chamber.

[0057] In practice, the temperature sensor collects the temperature value inside the constant pressure chamber 1 in real time and transmits the signal to the controller; the pressure sensor simultaneously collects the pressure value inside the chamber and feeds it back to the controller. The controller has pre-stored the set temperature (e.g., 37.0℃) and set pressure (positive or negative pressure value) required for the experiment. It compares the collected real-time data with the set values. If the temperature is lower than the set value, the controller increases the heating power of the constant temperature heating device 2; if the temperature is higher than the set value, it reduces the heating power or activates the cooling logic (if equipped). In terms of pressure control, when the measured pressure is lower than the positive pressure set value, the controller starts the external gas source 3 to fill the chamber with gas; when the measured pressure is higher than the set value or a negative pressure needs to be established, it starts the vacuum pump 9 to evacuate gas; when approaching or reaching the set pressure, it releases or replenishes an appropriate amount of gas by adjusting the opening of the pressure relief valve 4 to prevent overshoot and maintain pressure stability.

[0058] This closed-loop regulation mechanism can automatically respond to and continuously correct changes in the internal environment, ensuring that temperature and pressure remain within the set range and reducing deviations caused by environmental fluctuations or system inertia. Combined with the features of the constant pressure chamber 1, constant temperature heating device 2, insulation layer 5, shell 6, and sealing structure, it ensures stable temperature, humidity, and pressure conditions during long-term experiments, avoiding inconsistencies in experimental data caused by manual adjustment lag or errors. This control method enhances the system's automation and operational reliability, enabling in vitro cell culture and constant pressure testing to be conducted under repeatable and controllable conditions, thereby obtaining more comparable and reliable results for cell mechanical property studies.

[0059] As a preferred embodiment, in order to further improve the uniformity of temperature distribution and control stability in the constant pressure cavity 1, the constant temperature heating device 2 of this embodiment adopts a flexible or rigid electric heating element arranged around the outer wall of the constant pressure cavity 1, and a heating element is arranged inside and a corresponding heat-conducting structure is configured to achieve uniform heat transfer in the circumference and bottom of the constant pressure cavity 1.

[0060] In practice, the heating element can be machined to fit the outer wall of the constant pressure cavity 1. Flexible heating elements are suitable for slightly irregular shapes or curved surfaces, while rigid heating elements can provide stable installation and uniform heat output on regularly shaped cavities. The heating elements are arranged at certain intervals or in an array inside the heating element to ensure uniform heat release. The heat conduction structure can use a metal heat-conducting plate, heat-conducting film, or heat-conducting medium layer to efficiently conduct the heat generated by the heating element to the wall of the constant pressure cavity 1, and further radiate or convect into the cavity space. Since the heating area covers the circumference and bottom of the outer wall of the cavity, heat can enter the cavity in multiple directions, avoiding local overheating or low temperature.

[0061] During operation, the controller adjusts the power supply to the heating element based on the cavity temperature value fed back by the temperature sensor. The heat evenly released by the heating element is transferred to the cavity wall through the heat-conducting structure, making the radial temperature distribution within the cavity more uniform and significantly reducing the temperature difference between the bottom and the sidewalls. This structure, combined with the heat insulation effect of the aforementioned insulation layer 5 and shell 6, can reduce the impact of ambient temperature fluctuations on the cavity and improve the accuracy and stability of temperature maintenance.

[0062] The above are merely preferred embodiments of this utility model and do not limit the patent scope of this utility model. Any equivalent structural transformations made based on the inventive concept of this utility model and the contents of this utility model specification and drawings, or direct / indirect applications in other related technical fields, are included within the patent protection scope of this utility model.

Claims

1. A cell constant pressure testing system, characterized in that, include: The constant pressure chamber is used to hold the petri dish; A constant temperature heating device is evenly disposed on the outer wall of the constant pressure chamber to provide a preset constant temperature inside the constant pressure chamber; An external gas source or vacuum pump is connected to the constant pressure chamber through a pipeline to provide a constant positive pressure or a constant negative pressure to the constant pressure chamber; A pressure relief valve, connected to the constant pressure chamber, is used to maintain a preset pressure within the constant pressure chamber; Temperature sensor, used to collect temperature values ​​inside the constant pressure chamber; Pressure sensor, used to collect pressure values ​​in constant pressure chamber; The controller is electrically connected to the constant temperature heating device, the external air source or vacuum pump, the pressure relief valve, the temperature sensor, and the pressure sensor, respectively, and is used to adjust the working state of the constant temperature heating device, the external air source or vacuum pump, and the pressure relief valve according to the temperature and pressure values, so as to maintain the constant temperature and pressure in the constant pressure chamber.

2. The cell constant pressure testing system according to claim 1, characterized in that, It also includes a thermal insulation layer, which surrounds the outside of the constant temperature heating device.

3. The cell constant pressure testing system according to claim 2, characterized in that, It also includes a housing, in which the constant pressure chamber, constant temperature heating device and heat insulation layer are all disposed, and the heat insulation layer is located between the constant temperature heating device and the inner wall of the housing.

4. The cell constant pressure testing system according to any one of claims 1-3, characterized in that, The constant pressure chamber is a container with an opening at the top. A pressure-resistant cover is provided at the opening, and the pressure-resistant cover is detachably fixed to the opening by a quick-sealing locking structure.

5. The cell constant pressure testing system according to claim 4, characterized in that, The quick-sealing and locking structure includes at least one set of snap-fit ​​mechanisms or threaded tightening mechanisms distributed circumferentially along the opening of the constant pressure chamber, and works with a sealing ring to achieve quick disassembly and reliable sealing between the pressure-resistant cover and the opening.

6. The cell constant pressure testing system according to any one of claims 1-3, characterized in that, The external gas source provides a mixed gas containing a predetermined concentration of carbon dioxide to meet the gas environment requirements for cell culture.

7. The cell constant pressure testing system according to any one of claims 1-3, characterized in that, The controller is configured to receive detection signals from the temperature sensor and pressure sensor, and control the constant temperature heating device, external air source or vacuum pump and pressure relief valve according to the set parameters to achieve closed-loop regulation of temperature and pressure in the constant pressure chamber.

8. The cell constant pressure testing system according to any one of claims 1-3, characterized in that, The constant temperature heating device is a flexible or rigid electric heating element arranged around the outer wall of the constant pressure cavity. It has a heating element arranged inside and a heat-conducting structure configured to transfer heat evenly in the circumference and bottom of the constant pressure cavity.

9. The cell constant pressure testing system according to claim 3, characterized in that, The housing is also equipped with a control panel, and the control panel is communicatively connected to the controller; The control panel includes a control unit for setting preset temperature and preset pressure, and a display unit for displaying the temperature and pressure values ​​in the constant pressure chamber in real time.

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