Immobilized enzyme catalytic reaction device

Abstract

The utility model discloses a kind of immobilized enzyme catalytic reaction devices, including reactor, frame is supported on ground is installed on reactor, the upper end of reactor is detachably installed with upper cover, the upper end of upper cover is equipped with liquid inlet and electric cylinder, the lower end of reactor is equipped with liquid outlet, it is characterized by: the lateral wall of reactor is double-layer structure, from outside to inside, it is protective layer and heat preservation layer in order, the output end of electric cylinder is installed with catalytic assembly, electric cylinder is used to drive catalytic assembly to move along vertical direction, catalytic assembly is filled with enzyme catalytic reaction agent, and it is used to accelerate immobilized enzyme catalytic reaction efficiency, vibration assembly is installed on the inner wall of heat preservation layer of reactor, vibration assembly and catalytic assembly cross intersection distribution, the outer wall of heat preservation layer of reactor is equipped with temperature control device, temperature control device is used to accelerate enzyme reaction efficiency;Different particle size immobilized enzyme can be adapted by the "gradient aperture through hole" of cylindrical hollow shell, avoid enzyme molecule excessive leakage, improve catalytic rate.

Description

Technical Field

[0001] This utility model relates to the field of immobilized enzyme technology, specifically to an immobilized enzyme catalytic reaction device. Background Technology

[0002] Immobilized enzymes are enzymes that catalyze reactions within a defined spatial range and can be used repeatedly and continuously. While enzyme-catalyzed reactions typically occur in aqueous solutions, immobilized enzymes are water-soluble enzymes that have been treated by physical or chemical methods to render them insoluble in water but still possessing enzyme activity. Due to their advantages such as high efficiency, specificity, and environmental friendliness, immobilized enzymes have been widely used in food processing, pharmaceutical synthesis, and bioenergy.

[0003] In existing enzyme catalytic reaction devices, immobilized enzymes are often fixed in a single carrier or packed bed. The carrier structure is often poorly designed, such as having uniform pore size, which makes it easy for the substrate to form a diffusion boundary layer around the carrier. According to the mass transfer resistance law, the substrate must overcome the boundary layer resistance to reach the enzyme active site, resulting in insufficient contact between the enzyme and the substrate. At the same time, the product is difficult to detach from the enzyme surface quickly. According to the principle of enzyme inhibition kinetics, product accumulation will reduce the binding efficiency between the enzyme and the substrate, triggering a product inhibition effect and significantly reducing the catalytic rate.

[0004] Moreover, immobilized enzymes are inconvenient to replace and the operation process is complex. Traditional catalytic components are mostly monolithic structures, lacking detachable units. Based on the modular principle in engineering design, replacing enzymes requires disassembling a large number of parts, which is time-consuming and labor-intensive, and can easily damage the sealing of the device, causing many inconveniences in actual operation. Utility Model Content

[0005] The purpose of this invention is to provide an immobilized enzyme catalytic reaction device to solve the problems of low catalytic efficiency and inconvenient replacement of immobilized enzymes in the existing immobilized enzyme catalytic reaction device mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution: an immobilized enzyme catalytic reaction device, comprising a reaction vessel, a frame supported on the ground mounted on the reaction vessel, a detachable top cover mounted on the upper end of the reaction vessel, an inlet and an electric cylinder at the upper end of the top cover, and an outlet at the lower end of the reaction vessel, characterized in that: the side wall of the reaction vessel has a double-layer structure, consisting of a protective layer and a heat insulation layer from the outside to the inside; a catalytic component is mounted on the output end of the electric cylinder, the electric cylinder is used to drive the catalytic component to move vertically; the catalytic component is filled with an enzyme catalytic reactant and is used to accelerate the catalytic reaction efficiency of the immobilized enzyme; a vibration component is mounted on the inner wall of the heat insulation layer of the reaction vessel, the vibration component and the catalytic component are arranged in a cross shape; a temperature control device is provided on the outer wall of the heat insulation layer of the reaction vessel, the temperature control device is used to accelerate the enzyme reaction efficiency; The catalytic component includes a square block, a second groove, a cylindrical hollow shell, through holes, and a sealing cap. The bottom of the output end of the electric cylinder is fixed to a square block. The four horizontal side walls of the square block are provided with a second groove. The inner wall of the second groove is provided with a cylindrical hollow shell. The side walls of the cylindrical hollow shell are provided with multiple through holes with gradient apertures. One end of the cylindrical hollow shell is provided with a sealing cap. The vibration assembly includes an elastic support, an ultrasonic generator, and a conical amplitude transformer. The elastic support is fixed to the inner wall of the reactor by bolts. An ultrasonic generator is movably installed at the end of the elastic support away from the reactor, and a conical amplitude transformer is provided at the end of the ultrasonic generator away from the reactor.

[0007] Preferably, the catalyst assembly further includes a third groove, a three-dimensional cross bracket, a square hole, a first threaded hole, a fixing block, and a second threaded hole. The lower end of the square block has a cross-shaped third groove. The inner wall of the third groove is fitted with the three-dimensional cross bracket. The upper side wall of the three-dimensional cross bracket has a through-hole. The side wall of the cylindrical hollow shell near the square block has a through-hole. The upper side walls of the four sides of the square block are fixed with fixing blocks. The side walls of the fixing blocks have through-holes. The square block, the three-dimensional cross bracket, and the cylindrical hollow shell are threaded together by bolts inserted into the first and second threaded holes through the square hole at the top of the three-dimensional cross bracket.

[0008] By adopting the above technical solution, the third groove of the cross intersection provides precise positioning and support for the three-dimensional cross bracket, which greatly improves the rigidity of the overall structure. The bolt installation facilitates the rapid disassembly and assembly of the immobilized enzyme inside the catalytic component.

[0009] Preferably, the temperature control component includes a semi-circular ring, a flow guide tube, and a heat exchanger. Multiple semi-circular rings are fixed to the inner outer wall of the reactor. The inner outer wall of the reactor is provided with a spirally distributed flow guide tube. The flow guide tube is fixed to the inner outer wall of the reactor through the semi-circular rings. Both ends of the flow guide tube penetrate the outer wall of the reactor and are connected to a heat exchanger. The heat exchanger is installed at the upper end of the side wall frame of the reactor.

[0010] By adopting the above technical solution, the guide tubes are spirally distributed along the inner and outer walls of the reactor, which can uniformly cover most of the reactor body (from bottom to top), allowing the heating / cooling medium to exchange heat with the reaction system inside the reactor in all directions. This improves the heat exchange efficiency and also increases the reaction rate of the immobilized enzyme.

[0011] Preferably, the upper end of the cover is equipped with a temperature detector and a scale display.

[0012] Using the above technical solution, operators can obtain temperature and liquid level information simultaneously without opening the device.

[0013] Preferably, the lower end of the frame is provided with a base plate, which is fixed to the ground by bolts.

[0014] By adopting the above technical solution, the overall structural stability is enhanced and resistance to operational vibration interference is improved.

[0015] Preferably, a first groove is provided at the lower end of the top cover, the side wall of the reactor is installed on the inner wall of the first groove and fixed by screws, and a sealing gasket is provided between the inner wall of the first groove and the side wall of the reactor.

[0016] By adopting the above technical solution, the first groove is used to enhance the connection stability and avoid assembly misalignment, while the sealing gasket can improve the sealing performance and isolate the internal and external environments.

[0017] Preferably, a damping ring is provided between the elastic support and the movable end of the ultrasonic generator.

[0018] By adopting the above technical solution, the damping ring is used to attenuate vibration transmission and reduce the overall vibration of the equipment.

[0019] Preferably, the ultrasonic generator and temperature detector are connected to a Bluetooth module via Bluetooth.

[0020] Using the above technical solution, the temperature is detected in real time using a Bluetooth module, and the ultrasonic frequency emitted by the ultrasonic wave is controlled.

[0021] Compared with the prior art, the beneficial effects of this utility model are: 1. This immobilized enzyme catalytic reaction device can accommodate immobilized enzymes of different particle sizes through the "gradient aperture through-holes" of the cylindrical hollow shell. This ensures efficient entry of the substrate into the enzyme's active region while preventing excessive leakage of enzyme molecules. Simultaneously, the conical amplitude transformer and the cylindrical hollow shell are arranged in a cross (90°) pattern. Based on the principle of acoustic superposition, this creates "spatial complementarity" between ultrasonic energy and the catalytic components, covering most of the reaction vessel. The conical amplitude transformer focuses ultrasonic energy, enhancing the cavitation effect. The micro-jet generated by the cavitation bubble rupture impacts the surface of the immobilized enzyme, further eliminating mass transfer dead zones and increasing the contact area between the enzyme and the substrate. A spirally distributed guide tube is installed on the inner wall of the reaction vessel, increasing the heat exchange area (covering most of the vessel from bottom to top) based on the principles of heat conduction and convection. The heat exchanger circulates heating / cooling media through the guide tubes, and with real-time feedback from a temperature detector, the system temperature is quickly stabilized within the optimal range for the enzyme (±2℃) using the principle of negative feedback regulation, thereby improving the catalytic rate.

[0022] 2. This immobilized enzyme catalytic reaction device allows for easy disassembly of the cylindrical hollow outer shell by unscrewing the connecting bolts between the three-dimensional cross bracket and the fixing block, and the opening of the sealed cover to replace the enzyme. The operation is convenient and does not affect the overall structural stability of the device. The three-dimensional cross bracket is connected by a square hole and bolt fixing method. Combined with the design of the first groove of the upper cover and the sealing gasket, the overall rigidity of the device is greatly improved, the vibration resistance is significantly enhanced, and the loosening of components during long-term operation is prevented. Attached Figure Description

[0023] Figure 1 This is a three-dimensional structural diagram of an immobilized enzyme catalytic reaction device according to the present invention; Figure 2 This is a schematic diagram of the cross-sectional structure of the present invention; Figure 3 This is a three-dimensional structural diagram of the catalytic component of this utility model; Figure 4 This is a schematic diagram of the three-dimensional structure of the three-dimensional cross bracket and square block installation of this utility model; Figure 5 This utility model Figure 2 A magnified three-dimensional structural diagram of part A in the middle; Figure 6 This is a three-dimensional structural diagram of the temperature control component of this utility model. Figure 7 This is a three-dimensional top view of the structure of this utility model.

[0024] In the diagram: 1. Reactor; 2. Frame; 3. Top cover; 301. First groove; 4. Inlet; 5. Outlet; 6. Electric cylinder; 701. Square block; 702. Second groove; 703. Cylindrical hollow shell; 704. Through hole; 705. Sealing cover; 706. Third groove; 707. Three-dimensional cross bracket; 708. Square hole; 709. First threaded hole; 710. Fixing block; 711. Second threaded hole; 801. Elastic bracket; 802. Ultrasonic generator; 803. Conical amplitude transformer; 901. Semi-circular ring; 902. Guide tube; 903. Heat exchanger; 10. Temperature detector; 11. Scale display; 12. Base plate. Detailed Implementation

[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0026] To address the problems of low catalytic efficiency and inconvenient replacement of immobilized enzymes in existing immobilized enzyme catalytic reaction devices, the following technical solution is proposed. First, please refer to Figure 1-7 This utility model provides an embodiment: an immobilized enzyme catalytic reaction device, including a reaction vessel 1, a frame 2 supported on the ground mounted on the reaction vessel 1, a detachable top cover 3 mounted on the upper end of the reaction vessel 1, an inlet 4 and an electric cylinder 6 at the upper end of the top cover 3, and an outlet 5 at the lower end of the reaction vessel 1. The device is characterized in that: the side wall of the reaction vessel 1 has a double-layer structure, consisting of a protective layer and a heat insulation layer from the outside to the inside; a catalytic component is mounted on the output end of the electric cylinder 6, which drives the catalytic component to move vertically; the catalytic component is filled with an enzyme catalytic reactant and is used to accelerate the catalytic reaction efficiency of the immobilized enzyme; a vibration component is mounted on the inner wall of the heat insulation layer of the reaction vessel 1, and the vibration component and the catalytic component are arranged in a cross shape; a temperature control device is provided on the outer wall of the heat insulation layer of the reaction vessel 1, which is used to accelerate the enzyme reaction efficiency. The catalytic assembly includes a square block 701, a second groove 702, a cylindrical hollow shell 703, a through hole 704, and a sealing cap 705. The bottom of the output end of the electric cylinder 6 is fixed to the square block 701. The square block 701 has a second groove 702 on its four horizontal sidewalls. The inner wall of the second groove 702 is provided with a cylindrical hollow shell 703. The sidewall of the cylindrical hollow shell 703 has multiple through holes 704 with gradient apertures. A sealing cap 705 is provided at one end of the cylindrical hollow shell 703. The vibration assembly includes an elastic support 801, an ultrasonic generator 802, and a conical amplitude transformer 803. The elastic support 801 is fixed to the inner wall of the reactor 1 by bolts. The ultrasonic generator 802 is movably installed at the end of the elastic support 801 away from the reactor 1. The conical amplitude transformer 803 is provided at the end of the ultrasonic generator 802 away from the reactor 1.

[0027] The core function of the electric cylinder 6 is to convert rotary motion into linear telescopic motion to raise and lower the catalytic components. Other alternative solutions can be selected according to the needs of the scenario (such as thrust, precision, cost, environmental adaptability, etc.), such as pneumatic cylinders, linear motors, and electric push rods.

[0028] It is worth noting that the through holes 704 on the cylindrical hollow shell 703 can be customized according to the immobilized enzyme particle size (adjustable from 0.1 to 1 mm) to ensure that enzymes of different particle sizes can be released slowly and uniformly. The particle size of the immobilized enzyme (e.g., 0.1 to 1 mm) and the size of the substrate molecules (e.g., small molecule substrate <0.1 mm, large molecule substrate 0.5 to 2 mm) are used to set the through hole gradient to "small inside and large outside" (e.g., inner layer through holes 0.2 to 0.5 mm, outer layer through holes 0.8 to 1.2 mm), which is suitable for the rapid entry of small molecule substrates and the rapid detachment of large molecule products, reducing the thickness of the diffusion boundary layer. The cylindrical hollow shell 703 adopts "multi-level porous nanocomposite material" and is connected to the sealing cap 705 by snap-fit. Please see Figure 3-4 The catalytic assembly also includes a third groove 706, a three-dimensional cross bracket 707, a square hole 708, a first threaded hole 709, a fixing block 710, and a second threaded hole 711. The lower end of the square block 701 has a cross-shaped third groove 706. The three-dimensional cross bracket 707 is fitted to the inner wall of the third groove 706. The upper side wall of the three-dimensional cross bracket 707 has a through-hole 709. The side wall of the cylindrical hollow shell 703 near the square block 701 has a through-hole 708. The upper side walls of the four sides of the square block 701 are fixed to the fixing block 710. The side wall of the fixing block 710 has a through-hole 711. The square block 701, the three-dimensional cross bracket 707, and the cylindrical hollow shell 703 are threaded together by bolts inserted into the first threaded hole 709 and the second threaded hole 711 through the square hole 708 at the top of the three-dimensional cross bracket 707.

[0029] It is worth noting that: anti-loosening nuts and elastic washers (made of nitrile rubber) are added between the bolt and the first threaded hole 709 and the second threaded hole 711 to counteract the loosening caused by ultrasonic vibration through elastic pre-tightening force; at the same time, a ceramic wear-resistant coating (thickness 50μm) is sprayed on the contact area between the three-dimensional cross bracket 707 and the square hole 708.

[0030] Please see Figure 1 and Figure 6 The temperature control component includes a semi-circular ring 901, a flow guide pipe 902, and a heat exchanger 903. Multiple semi-circular rings 901 are fixed to the inner outer wall of the reactor 1. The inner outer wall of the reactor 1 is provided with spirally distributed flow guide pipes 902. The flow guide pipes 902 are fixed to the inner outer wall of the reactor 1 through the semi-circular rings 901. Both ends of the flow guide pipes 902 penetrate the outer wall of the reactor 1 and are connected to the heat exchanger 903. The heat exchanger 903 is installed on the upper end of the side wall frame 2 of the reactor 1.

[0031] Please see Figure 1 The upper end of the cover 3 is equipped with a temperature detector 10 and a scale display 11.

[0032] It is worth noting that when one end of the temperature detector 10 and the scale display 11 passes through the top cover 3, a sealing gasket is installed on the contact surface to ensure the airtightness of the entire device during the reaction.

[0033] Please see Figure 1 The lower end of the frame 2 is provided with a base plate 12, which is fixed to the ground by bolts.

[0034] Please see Figure 1-2 The lower end of the upper cover 3 is provided with a first groove 301. The side wall of the reactor 1 is installed on the inner wall of the first groove 301 and fixed with screws. A sealing gasket is provided between the inner wall of the first groove 301 and the side wall of the reactor 1.

[0035] Please see Figure 5 A damping ring is provided between the movable end of the elastic support 801 and the ultrasonic generator 802.

[0036] Please see Figure 1 and Figure 5 The ultrasonic generator 802 and the temperature detector 10 are connected to the Bluetooth module via Bluetooth.

[0037] It is worth noting that the Bluetooth module (not shown in the figure) is not only used for remote control, but also for receiving real-time data from the temperature detector 10. When the temperature deviates from the optimal range of the enzyme (e.g., ±2℃), it automatically reduces the ultrasonic power (e.g., from 30W to 15W) to avoid damage to the immobilized enzyme due to the double effect of high temperature and strong ultrasound.

[0038] In use, the frame 2 is fixed in a suitable position by the base plate 12, and the substrate solution is added into the reaction vessel 1 through the liquid inlet 4 of the top cover 3. The scale display 11 displays the liquid level in real time to ensure accurate feed. At the same time, the immobilized enzyme is filled into the cylindrical hollow shell 703 of the catalytic component and sealed by the sealing cover 705. The gradient aperture through holes 704 of the cylindrical hollow shell 703 can be adapted to immobilized enzymes of different particle sizes, which ensures that the substrate enters the enzyme activity area efficiently and avoids excessive leakage of enzyme molecules. Then, the electric cylinder 6 drives the square block 701 and the cylindrical hollow shell 703 to descend to the preset depth in the reaction solution. The height is adjusted in real time according to the amount of material to avoid enzyme floating and waste. Then, the ultrasonic generator 802 is activated via Bluetooth on the mobile phone. The ultrasonic energy is focused by the conical amplitude transformer 803 and transmitted to the reaction system, triggering the cavitation effect and breaking the diffusion boundary layer of the substrate around the immobilized enzyme. At the same time, the flexible support of the elastic support 801 and the damping effect of the damping ring concentrate the ultrasonic vibration in the reaction area and avoid device resonance. Since the conical amplitude transformer 803 and the cylindrical hollow shell 703 are arranged in a cross (90°) pattern, based on the principle of acoustic superposition, the ultrasonic energy and the catalytic components form a "spatial complementarity", covering most of the area inside the reactor 1. The conical amplitude transformer 803 focuses the ultrasonic energy, enhances the cavitation effect intensity, and uses the micro-jet generated by the cavitation bubble rupture to impact the immobilized enzyme surface, further eliminating mass transfer dead zones, increasing the contact area between the enzyme and the substrate, and thus improving the catalytic rate. Next, the temperature of the reactor 1 is detected by the temperature detector 10. Since the inner and outer walls of the reactor 1 are equipped with spirally distributed guide pipes 902, the heat exchange area is increased based on the principle of heat conduction and convection heat transfer (covering most of the area of ​​the reactor body from bottom to top). The heat exchanger 903 circulates the heating / cooling medium through the guide pipes 902. With the real-time feedback from the temperature detector 10, the system temperature is quickly stabilized within the optimal range of the enzyme (within ±2℃) by using the principle of negative feedback regulation. Finally, the first groove 301 is opened at the lower end of the upper cover 3, and it is fixed to the side wall of the reactor 1 with screws and a sealing gasket is added. The groove positioning principle is used to enhance the connection rigidity. The three-dimensional cross bracket 707 passes through the square hole 708 and is fixed to the fixing block 710 with bolts (with anti-loosening nuts and elastic washers). The mechanical locking principle is used to offset the vibration stress. The lower end of the frame 2 is fixed to the ground with bolts to the base plate 12 to reduce the overall vibration amplitude and improve the anti-interference ability. In addition, depending on the size of the immobilized enzyme particles, the connecting bolts between the three-dimensional cross bracket 707 and the fixing block 710 can be unscrewed to disassemble the cylindrical hollow shell 703 for replacement (through holes 704 for different particle sizes). If the immobilized enzyme needs to be replaced, the sealing cover 705 can be opened to complete the enzyme replacement. The operation is convenient and does not affect the overall structural stability of the device.

[0039] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An immobilized enzyme catalytic reaction apparatus, comprising a reaction vessel (1), a frame (2) supported on the ground mounted on the reaction vessel (1), a top cover (3) detachably mounted on the upper end of the reaction vessel (1), an inlet (4) and an electric cylinder (6) provided at the upper end of the top cover (3), and an outlet (5) provided at the lower end of the reaction vessel (1), characterized in that: The side wall of the reactor (1) has a double-layer structure, consisting of a protective layer and a heat insulation layer from the outside to the inside. A catalytic component is installed at the output end of the electric cylinder (6). The electric cylinder (6) is used to drive the catalytic component to move vertically. The catalytic component is filled with an enzyme catalytic reaction agent and is used to accelerate the efficiency of the immobilized enzyme catalytic reaction. A vibration component is installed on the inner wall of the heat insulation layer of the reactor (1). The vibration component and the catalytic component are arranged in a cross shape. A temperature control device is provided on the outer wall of the heat insulation layer of the reactor (1). The temperature control device is used to accelerate the efficiency of the enzyme reaction. The catalytic assembly includes a square block (701), a second groove (702), a cylindrical hollow shell (703), a through hole (704), and a sealing cap (705). The bottom of the output end of the electric cylinder (6) is fixedly connected to the square block (701). The four horizontal side walls of the square block (701) are provided with the second groove (702). The inner wall of the second groove (702) is provided with the cylindrical hollow shell (703). The side wall of the cylindrical hollow shell (703) is provided with multiple through holes (704). The through holes (704) are of gradient diameter. A sealing cap (705) is provided at one end of the cylindrical hollow shell (703). The vibration assembly includes an elastic support (801), an ultrasonic generator (802), and a conical amplitude transformer (803). The inner wall of the reactor (1) is fixed with an elastic support (801) by bolts. An ultrasonic generator (802) is movably installed at the end of the elastic support (801) away from the reactor (1). A conical amplitude transformer (803) is provided at the end of the ultrasonic generator (802) away from the reactor (1).

2. The immobilized enzyme catalytic reaction device according to claim 1, characterized in that: The catalyst assembly also includes a third groove (706), a three-dimensional cross bracket (707), a square hole (708), a first threaded hole (709), a fixing block (710), and a second threaded hole (711). The lower end of the square block (701) has a cross-shaped third groove (706). The three-dimensional cross bracket (707) is fitted to the inner wall of the third groove (706). The upper side wall of the three-dimensional cross bracket (707) has a through-hole (709). The cylindrical hollow shell (703) is close to... A square hole (708) is provided through one end of the side wall of the near square block (701). A fixing block (710) is fixed to the upper side wall of the four sides of the square block (701). A second threaded hole (711) is provided through the side wall of the fixing block (710). The square block (701), the three-dimensional cross bracket (707) and the cylindrical hollow shell (703) are threaded by bolts inserted into the first threaded hole (709) and the second threaded hole (711) through the upper part of the three-dimensional cross bracket (707) through the square hole (708).

3. The immobilized enzyme catalytic reaction device according to claim 1, characterized in that: The temperature control assembly includes a semi-circular ring (901), a flow guide pipe (902), and a heat exchanger (903). Multiple semi-circular rings (901) are fixed to the inner outer wall of the reactor (1). The inner outer wall of the reactor (1) is provided with spirally distributed flow guide pipes (902). The flow guide pipes (902) are fixed to the inner outer wall of the reactor (1) through the semi-circular rings (901). The two ends of the flow guide pipes (902) penetrate the outer wall of the reactor (1) and are connected to the heat exchanger (903). The heat exchanger (903) is installed at the upper end of the side wall frame (2) of the reactor (1).

4. The immobilized enzyme catalytic reaction device according to claim 1, characterized in that: The upper end of the cover (3) is equipped with a temperature detector (10) and a scale display (11). The temperature detector (10) is used to detect the reaction temperature in real time and provide feedback.

5. The immobilized enzyme catalytic reaction device according to claim 1, characterized in that: The lower end of the frame (2) is provided with a base plate (12), which is fixed to the ground by bolts.

6. The immobilized enzyme catalytic reaction apparatus according to claim 1, characterized in that: The lower end of the cover (3) is provided with a first groove (301). The side wall of the reactor (1) is installed on the inner wall of the first groove (301) and fixed with screws. A sealing gasket is provided between the inner wall of the first groove (301) and the side wall of the reactor (1).

7. The immobilized enzyme catalytic reaction device according to claim 1, characterized in that: A damping ring is provided between the movable end of the elastic support (801) and the ultrasonic generator (802).

8. The immobilized enzyme catalytic reaction device according to claim 1, characterized in that: The ultrasonic generator (802) and the temperature detector (10) are connected to the Bluetooth module via Bluetooth.

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