Defoaming machine for cell observation dish

Abstract

The utility model relates to a kind of cell observation dish defoaming machine, belong to biological equipment field, the defoaming machine includes box, uniform flow membrane, fan and heating unit;The inner chamber middle part of the box is horizontally provided with uniform flow membrane, the inner chamber of the box is divided into upper cavity and lower cavity by the uniform flow membrane, the top of the box is hollowly arranged, and the heating unit is assembled in the top inner side of box, the fan is suspended by support and set in the upper cavity of box;Supporting rod for carrying cell observation dish is fixed on the inner wall of the lower cavity of the box, and the lower side of the box is provided with flow hole extending to bottom. The utility model is equipped with heating unit and fan, can heat airflow, and make airflow act on cell culture dish downward, by setting uniform flow membrane, airflow can uniformly act on each hole position of cell observation dish, to improve defoaming success rate.

Description

Technical Field

[0001] This utility model belongs to the field of biological equipment technology, specifically relating to a defoaming machine for cell observation dishes. Background Technology

[0002] In protein activity assays in the biomedical field, one of the core steps is to determine protein activity by observing whether it promotes or inhibits cell growth. This requires precise measurement of cell density or number. Currently, the commonly used method in the industry is to add cell staining agents to observation dishes (such as 96-well plates) containing the cells to be tested, and then use an ELISA reader to detect the absorbance, thereby achieving quantitative analysis of cell number.

[0003] However, due to the small pore size and limited volume of cell observation dishes such as 96-well plates, air bubbles are easily generated during sample injection. These air bubbles can adhere to the inner wall of the plate or be suspended in the detection solution. When the detection light path of the ELISA reader passes through the air bubbles, light refraction and scattering occur, leading to significant deviations in absorbance detection data and directly affecting the accuracy of cell counting results.

[0004] In existing technologies, methods such as manual shaking or static defoaming are often used to address this type of bubble problem. These methods are not only inefficient and cannot meet the needs of batch testing, but manual operation can also easily lead to cell contamination or overflow of the test solution, further affecting the reliability of the experiment. Utility Model Content

[0005] In view of the shortcomings of the prior art, this utility model proposes a defoaming machine for cell observation dishes.

[0006] The specific technical solution adopted by this utility model is as follows:

[0007] A defoaming machine for cell observation dishes includes a box body, a flow equalization membrane, a fan, and a heating unit; the flow equalization membrane is horizontally arranged in the middle of the inner cavity of the box body, the flow equalization membrane divides the inner cavity of the box body into an upper cavity and a lower cavity, the top of the box body is hollowed out, and the heating unit is assembled on the inner side of the top of the box body; the fan is suspended in the upper cavity of the box body by a bracket.

[0008] The lower cavity of the box is fixed with a support rod for holding the cell observation dish, and the lower side of the box has a drain hole extending to the bottom.

[0009] Preferably, the flow equalization membrane is made of PET material.

[0010] Preferably, the heating unit includes a power supply and multiple resistance wires connected in series. The power supply and the resistance wires form a closed loop through a wire, and the resistance wires are arranged in an array on the inner side of the top of the housing.

[0011] Preferably, a control panel is provided on the outside of the housing, and the control panel is electrically connected to the power supply of the heating unit and the drive power supply of the fan.

[0012] Preferably, the front side of the box has a door opening, and the area above the support rod is provided with a space for taking out and placing the cell observation dish to be fully exposed. The front side of the box is hinged with a door that matches the door opening.

[0013] Preferably, the door is provided with a glass observation window.

[0014] Preferably, the edges of the cabinet door are provided with sealing strips.

[0015] Preferably, the four side walls of the box are provided with drainage holes.

[0016] Preferably, the drain hole is arched.

[0017] Preferably, the cell observation dish is provided with a mesh sealing plate.

[0018] Compared with the prior art, the present invention has at least one of the following advantages or beneficial effects:

[0019] I. This utility model, by setting up a heating unit and a fan, can heat the airflow and make the airflow act downward on the cell culture dish. By setting up a uniform flow membrane, the airflow can act evenly on each well of the cell observation dish, thereby improving the defoaming success rate.

[0020] Second, in this utility model, the design of the box door facilitates the quick placement and removal of cell observation dishes, and the transparent observation window allows for real-time monitoring of the defoaming progress.

[0021] Third, this utility model replaces manual operation with hot air defoaming, avoiding the risk of cell contamination and test solution overflow; the high bubble removal rate can effectively eliminate light path interference, reduce the detection error of the enzyme-linked immunosorbent assay (ELISA) reader to within ±2%, and significantly improve the reliability of cell counting results. Attached Figure Description

[0022] Figure 1 This is a cross-sectional view of the defoaming machine for cell observation dishes in this embodiment of the present invention;

[0023] Figure 2 This is a front view of the defoaming machine for cell observation dishes in this embodiment of the present invention;

[0024] Figure 3 This is a top view of a cell observation dish without a mesh sealing plate in an embodiment of this utility model;

[0025] Figure 4This is a top view of a cell observation dish with a mesh sealing plate in an embodiment of the present invention;

[0026] In the diagram: 1. Box body; 2. Uniform flow membrane; 3. Heating unit; 4. Fan; 5. Support rod; 6. Stand; 7. Cell observation dish; 8. Control panel; 9. Box door; 10. Observation window; 11. Drain hole; 12. Mesh sealing plate. Detailed Implementation

[0027] To make the objectives and advantages of this utility model clearer, the following detailed description is provided in conjunction with embodiments. It should be understood that the following text is merely used to describe one or more specific embodiments of this utility model and does not strictly limit the scope of protection specifically claimed by this utility model.

[0028] See Figures 1-3 As shown, this embodiment discloses a defoaming machine for cell observation dishes. Specifically, the defoaming machine for cell observation dishes includes a box body 1, a flow equalization membrane 2, a fan 4, and a heating unit 3. The box body 1 has a cuboid structure. The flow equalization membrane 2 is horizontally fixed in the middle of the inner cavity of the box body 1. The flow equalization membrane 2 divides the inner cavity of the box body 1 into an upper cavity (air source cavity) and a lower cavity (defoaming cavity). The top of the box body 1 is hollowed out to allow air to enter from the top of the box body 1. The heating unit 3 is assembled on the inner side of the top of the box body 1. The fan 4 is suspended in the upper cavity by a bracket 6. The fan 4 enables the airflow to flow towards the flow equalization membrane 2. Specifically, the fan 4 can be a fan.

[0029] Specifically, the lower cavity of the aforementioned box 1 is symmetrically fixed with support rods 5 for horizontally supporting cell observation dishes 7 (e.g., 96-well plates) (e.g., two support rods 5 are respectively provided on the left and right inner walls). The cross-sectional dimension of the inner cavity of the box 1 is larger than the size of the cell observation dish 7. The lower side walls of the box 1 are all provided with vent holes 11 extending to the bottom to form a three-dimensional airflow circulation channel. This ensures that the hot air delivered by the upper cavity is uniformly acted on by the lower cavity and then quickly discharged through the gaps in the support rods 5 from the vent holes 11, avoiding airflow turbulence that could lead to the generation of new bubbles. Furthermore, the vent holes 11 are arched, which can reduce airflow resistance, improve defoaming efficiency, and reduce airflow turbulence.

[0030] In a preferred embodiment of this invention, the flow equalization membrane 2 is made of PET material. This material provides excellent high-temperature resistance and airflow uniformity. The flow equalization membrane 2 can convert the airflow generated by the fan 4 into a uniform laminar flow, preventing excessively high local wind speeds that could cause splashing of the test liquid. Furthermore, a mesh sealing plate 12 can be placed over the cell observation dish 7 to further guide the airflow to act uniformly on each well. The combination of the flow equalization membrane 2 and the mesh sealing plate 12 ensures that the airflow acts uniformly on each well of the cell observation dish 7, resulting in a high defoaming success rate.

[0031] In a preferred embodiment of this utility model, the heating unit 3 includes a power supply and multiple resistance wires connected in series. The power supply and the resistance wires form a closed loop through a wire, and the resistance wires are arranged in an array on the inner side of the top of the housing 1 to achieve uniform heating. A control panel 8 is provided on the outer side of the housing 1. The control panel 8 is electrically connected to the power supply of the heating unit 3 and the drive power supply of the fan 4. It can independently control the start and stop of the heating unit 3, the start and stop of the fan 4, and the wind speed adjustment (adjustment range is 0.24~0.36 m / s). The control panel 8 also has temperature setting (temperature range is 30-60℃) and display functions, and can realize functions such as fan 4 start and stop, wind speed adjustment, heating start and stop, and temperature setting (30-60℃).

[0032] In a preferred embodiment of this utility model, a door opening is provided on the front side of the box body 1 (the door opening can be set to correspond to the lower cavity). The door opening is set above the support rod 5 to fully expose the space for taking out and placing the cell observation dish 7. The front side of the box body 1 is hinged to a box door 9 that matches the door opening. The edge of the box door 9 is provided with a sealing strip so that the box door 9 can be sealed to the door opening when closed (relative sealing is sufficient, absolute sealing is not required). The box door 9 is provided with a glass observation window 10 to facilitate real-time observation of the defoaming process.

[0033] Specifically, in use, after covering the cell observation dish 7 with the mesh sealing plate 12, open the chamber door 9 and place the cell observation dish 7 on the support rod 5, then close the chamber door 9; then, set the temperature to 45℃, the wind speed to 0.3m / s, and the timer to 2 minutes through the control panel 8, and click the start button; the airflow is heated by the heating unit 3, and the fan 4 blows the heated airflow toward the uniform flow membrane 2, which distributes the airflow into a laminar flow that acts vertically on the surface of the cell observation dish 7; the operator observes the bubble bursting through the observation window 10. If there are still residual bubbles after 2 minutes, the timer can be extended by 1 minute through the timer button; after the defoaming is completed, stop the machine, open the chamber door 9 and take out the cell observation dish 7, and then proceed with the subsequent enzyme-linked immunosorbent assay (ELISA) detection.

[0034] The above are merely preferred embodiments of this utility model. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of this utility model, and these improvements and modifications should also be considered within the scope of protection of this utility model. Structures, devices, and operating methods not specifically described or explained in this utility model, unless otherwise specified or limited, shall be implemented using conventional methods in the field.

Claims

1. A defoaming machine for cell observation dishes, characterized in that, The device includes a housing, a flow equalization membrane, a fan, and a heating unit. A flow equalization membrane is horizontally arranged in the middle of the inner cavity of the housing, which divides the inner cavity of the housing into an upper cavity and a lower cavity. The top of the housing is hollowed out, and the heating unit is assembled inside the top of the housing. The fan is suspended in the upper cavity of the housing by a bracket. The lower cavity of the box is fixed with a support rod for holding the cell observation dish, and the lower side of the box has a drain hole extending to the bottom.

2. The defoaming machine for cell observation dishes as described in claim 1, characterized in that, The flow equalization membrane is made of PET material.

3. The defoaming machine for cell observation dishes as described in claim 1, characterized in that, The heating unit includes a power supply and multiple resistance wires connected in series. The power supply and the resistance wires form a closed loop through a wire, and the resistance wires are arranged in an array on the inner side of the top of the housing.

4. The defoaming machine for cell observation dishes as described in claim 3, characterized in that, A control panel is provided on the outside of the housing, and the control panel is electrically connected to the power supply of the heating unit and the drive power supply of the fan.

5. The defoaming machine for cell observation dishes as described in claim 1, characterized in that, The front of the box has a door opening, and the area above the support rod is provided to fully expose the space for placing and taking out the cell observation dish. The front of the box is hinged to a door that matches the door opening.

6. The defoaming machine for cell observation dishes as described in claim 5, characterized in that, The box door is equipped with a glass observation window.

7. The defoaming machine for cell observation dishes as described in claim 5, characterized in that, The edges of the cabinet door are fitted with sealing strips.

8. The defoaming machine for cell observation dishes as described in claim 1, characterized in that, Drainage holes are provided on all four sides of the box.

9. The defoaming machine for cell observation dishes as described in claim 1, characterized in that, The drain hole is arched.

10. The defoaming machine for cell observation dishes as described in claim 1, characterized in that, The cell observation dish is equipped with a mesh sealing plate.

Images