A specialized culture dish for observing dynamic growth of brain organoids

Abstract

The utility model discloses a special culture dish for observing the dynamic growth of brain organoids, including culture dish main part, the culture dish main part top is equipped with the even arrangement of a plurality of culture holes, the culture dish main part top is equipped with fixed lid, the fixed lid top fixedly connected with even arrangement a plurality of liquid injection channel, the liquid injection channel top is equipped with the closure component, be equipped with sealing component between the fixed lid and culture dish main part, the closure component includes with the first support board of liquid injection channel one side fixed connection, with the first support board top fixed connection's pivot and with the second support board of pivot outside link, the utility model discloses a culture dish main part, culture hole, fixed lid, liquid injection channel and closure component are arranged, when using, the sealing cover of existing culture dish lacks the connecting structure, and the size of sealing washer is same with the size of culture dish, and the problem that the resource is wasted easily, thereby leading to the practicability reduction problem of easy loss situation.

Description

Technical Field

[0001] This utility model relates to the field of brain organoid culture dish technology, specifically a special culture dish for observing the dynamic growth of brain organoids. Background Technology

[0002] Brain organoids are three-dimensional miniature brain tissue models cultured in vitro using stem cells. They can simulate some of the structure and function of the real brain and are widely used in the fields of neural development, disease mechanism research, and drug testing. Their core value lies in providing scientists with a highly controllable research tool that closely resembles real brain tissue, which is particularly significant in exploring complex brain diseases such as autism and Alzheimer's disease. Culture dishes are required for culturing brain organoids.

[0003] Existing patent CN220224189U discloses an organoid culture device, belonging to the field of medical device technology. This device includes a culture dish body with several culture chambers whose bottoms are sealed; a culture dish lid with a hollow liquid injection channel penetrating the lid body; the liquid injection channel extending downwards from the lid body to form a guide section, the bottom of which is an elliptical inclined surface with its bottom end close to the sidewall of the culture chamber; and a filter membrane, specifically a bacterial filter membrane, is disposed within the liquid injection channel. The organoid culture device provided in this application can effectively avoid bacterial contamination problems caused by frequent opening and closing of the culture dish while ensuring gentle reagent addition.

[0004] Based on the search of the aforementioned patents and the findings of existing petri dishes, while current petri dishes can be used to cultivate brain organoids and inject reagents through the injection channel, and the injection channel can be sealed with a sealing cap, the sealing cap lacks a connecting structure and is easily lost. Moreover, the size of the sealing gasket is the same as the size of the petri dish, which easily wastes resources and reduces its practicality. Summary of the Invention

[0005] The purpose of this invention is to provide a special culture dish for observing the dynamic growth of brain organoids, in order to solve the problems mentioned in the background art. Although existing culture dishes can cultivate brain organoids and inject reagents through the injection channel, and the injection channel is sealed by a sealing cap, the sealing cap lacks a connecting structure and is easily lost. Moreover, the size of the sealing gasket is the same as the size of the culture dish, which easily wastes resources and reduces its practicality.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a special culture dish for observing the dynamic growth of brain organoids, comprising a culture dish body, a plurality of culture holes evenly arranged on the top of the culture dish body, a fixed cover on the top of the culture dish body, a plurality of liquid injection channels evenly arranged fixedly connected to the top of the fixed cover, a sealing component on the top of the liquid injection channels, and a sealing component between the fixed cover and the culture dish body; The sealing component includes a first support plate fixedly connected to one side of the injection channel, a rotating shaft fixedly connected to the top of the first support plate, a second support plate connected to the outside of the rotating shaft, a sealing cover fixedly connected to one side of the second support plate, a first mounting groove opened at the bottom of the sealing cover, a second mounting groove opened at the top side of the injection channel, a first magnet fixedly connected to the inner wall of the first mounting groove, and a second magnet fixedly connected to the inner wall of the second mounting groove. The rotating shaft is rotatably connected to the second support plate.

[0007] Preferably, the sealing component includes a plurality of sealing grooves evenly arranged on the top of the petri dish body, a plurality of pressure rings evenly arranged on the bottom of the fixed cover, a first sealing ring fixedly connected to the bottom of the pressure rings, a plurality of sliding grooves arranged circumferentially on the bottom of the sealing grooves, a sliding rod connected to the sliding grooves, a fixed ring fixedly connected to the top of the sliding rod, a second sealing ring fixedly connected to the top of the fixed ring, and a first spring sleeved on the outside of the sliding rod. The pressure rings are inserted into the sealing grooves, and the two ends of the first spring are fixedly connected to the bottom of the sealing grooves and the bottom of the fixed rings, respectively.

[0008] Preferably, the fixed cover has movable grooves on both sides, a movable rod is slidably connected in the movable groove, a second spring is sleeved on the outside of the movable rod, a gripping plate is fixedly connected to one end of the movable rod, a locking rod is fixedly connected to one side of the gripping plate, a locking groove is opened on both sides of the culture dish body, the locking rod is inserted into the locking groove, and the movable rod is slidably connected to the movable groove.

[0009] Preferably, a lever is fixedly connected to the top of the closed cover, and a lever plate is fixedly connected to the top of the lever.

[0010] Preferably, a limiting groove is formed at the bottom of the slide bar, and a limiting rod is fixedly connected to the bottom of the slide groove, with the limiting rod slidably connected to the limiting groove.

[0011] Preferably, a bacterial filter membrane is fixedly connected to the inner wall of the injection channel.

[0012] Preferably, a third sealing ring is fixedly connected to one side of the bottom of the fixing ring.

[0013] Compared with the prior art, the beneficial effects of this utility model are: 1. By setting up a culture dish body, culture wells, a fixed cap, a liquid injection channel, and a sealing component, the fixed cap can seal the liquid injection channel to prevent bacterial infection. By rotating the lever, the sealing cap can be rotated to open the liquid injection channel for easy reagent injection. At the same time, the sealing cap will not detach from the liquid injection channel to prevent loss. When the sealing cap is reset, the first and second magnets can attract and fix each other to prevent the sealing cap from rotating, which can improve the protection effect and thus greatly improve practicality. 2. By incorporating a sealing component, when the fixed cover is attached to the main body of the culture dish, the pressure ring can be inserted into the sealing groove, allowing the first sealing ring to contact the second sealing ring and compress the second sealing ring, causing it to move downwards. This compresses the spring, causing it to deform. The reaction force generated by the spring deformation ensures that the second sealing ring and the first sealing ring are in close contact, thus achieving a sealing effect. This also reduces the cost of sealing and further improves practicality. Attached Figure Description

[0014] Figure 1 A three-dimensional structural schematic diagram provided for this utility model; Figure 2 The left view provided for this utility model; Figure 3 Provided by this utility model Figure 2 A three-dimensional cross-sectional view at point AA; Figure 4 Provided by this utility model Figure 3 Enlarged view of point B in the middle; Figure 5 Provided by this utility model Figure 3 Enlarged view of point C in the middle; Figure 6 Provided by this utility model Figure 3 Enlarged view of point D in the middle.

[0015] In the diagram: 1. Petri dish body; 11. Culture well; 12. Fixing cap; 13. Liquid injection channel; 21. First support plate; 22. Rotating shaft; 23. Second support plate; 24. Sealing cap; 25. First mounting groove; 26. Second mounting groove; 27. First magnet; 28. Second magnet; 31. Sealing groove; 32. Pressure ring; 33. First sealing ring; 34. Sliding groove; 35. Sliding rod; 36. Fixing ring; 37. Second sealing ring; 38. First spring; 41. Moving groove; 42. Moving rod; 43. Second spring; 44. Grip plate; 45. Locking rod; 46. Locking groove; 51. Actuating rod; 52. Actuating plate; 61. Limiting groove; 62. Limiting rod; 71. Bacterial filter membrane; 81. Third sealing ring. Detailed Implementation

[0016] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0017] Please see Figure 1 , Figure 2 , Figure 3 , Figure 4 as well as Figure 6This utility model provides a technical solution: a special culture dish for observing the dynamic growth of brain organoids, comprising a culture dish body 1, a plurality of culture holes 11 evenly arranged on the top of the culture dish body 1, a fixed cover 12 on the top of the culture dish body 1, a plurality of liquid injection channels 13 evenly arranged fixedly connected to the top of the fixed cover 12, a sealing component on the top of the liquid injection channels 13, and a sealing component between the fixed cover 12 and the culture dish body 1. The sealing component includes a first support plate 21 fixedly connected to one side of the liquid injection channel 13, a rotating shaft 22 fixedly connected to the top of the first support plate 21, a second support plate 23 connected to the outside of the rotating shaft 22, and a sealing cover 24 fixedly connected to one side of the second support plate 23. The sealing cover 24 has a first mounting groove 25 at the bottom, a second mounting groove 26 on one side of the top of the injection channel 13, a first magnet 27 fixedly connected to the inner wall of the first mounting groove 25, and a second magnet 28 fixedly connected to the inner wall of the second mounting groove 26. A rotating shaft 22 is rotatably connected to a second support plate 23. The material of the fixing cover 12 can be acrylic, which facilitates observation of the dynamic growth of brain organoids. The sealing cover 24 can seal the injection channel 13, preventing impurities from entering. Simultaneously, the sealing cover 24 can rotate to one side, exposing the injection channel 13 for easy drug injection. Furthermore, the sealing cover 24 will not detach from the injection channel 13, preventing loss. When in position, the first magnet 27 and the second magnet 28 can attract and fix each other, preventing the sealing cover 24 from rotating arbitrarily. The fixing cover 12 has movable grooves 41 on both sides, with a movable rod 42 slidably connected within each groove 41. A second spring 43 is sleeved on the outside of the movable rod 42. A grip plate 44 is fixedly connected to one end of the movable rod 42, and a locking rod 45 is fixedly connected to one side of the grip plate 44. The culture dish body 1 has locking slots 46 on both sides, with the locking rod 45 inserted into the slots 46. The movable rod 42 is slidably connected to the movable groove 41. Pulling the grip plate 44 can move the movable rod 42 and the locking rod 45. The two ends of the second spring 43 are fixedly connected to the inner walls of the movable rod 42 and the movable groove 41, respectively. The movement causes the second spring 43 to deform, and the return of the second spring 43 allows the locking rod 45 to be inserted into the locking groove 46, which can connect and fix the fixing cover 12 to the petri dish body 1, and can protect the culture hole 11. The top of the sealing cover 24 is fixedly connected to the actuating rod 51, and the top of the actuating rod 51 is fixedly connected to the actuating plate 52. The actuating plate 52 can drive the actuating rod 51 to move, which in turn can facilitate the rotation of the sealing cover 24. The inner wall of the liquid injection channel 13 is fixedly connected to the bacterial filter membrane 71. The liquid injection channel 13 can serve as a ventilation channel to realize gas exchange between the inside and outside of the petri dish. During the gas exchange process, bacteria in the external environment are intercepted by the bacterial filter membrane 71, ensuring a sterile environment in the petri dish.

[0018] Please see Figure 1 , Figure 2 , Figure 3 as well as Figure 5 The sealing components include multiple sealing grooves 31 evenly arranged on the top of the petri dish body 1, multiple pressure rings 32 evenly arranged on the bottom of the fixed cover 12, a first sealing ring 33 fixedly connected to the bottom of the pressure rings 32, multiple sliding grooves 34 arranged circumferentially on the bottom of the sealing grooves 31, a sliding rod 35 connected to the sliding grooves 34, a fixed ring 36 fixedly connected to the top of the sliding rod 35, a second sealing ring 37 fixedly connected to the top of the fixed ring 36, and a first spring 38 sleeved on the outside of the sliding rod 35. The pressure rings 32 are inserted into the sealing grooves 31, and the two ends of the first spring 38 are fixedly connected to the bottom of the sealing grooves 31 and the bottom of the fixed rings 36, respectively. When the fixed cover 12 contacts the petri dish body 1, the pressure rings 32 can be inserted into the sealing grooves 31, causing the first sealing rings 33 to contact the second sealing rings 37 and push the second sealing rings 37 to deform, causing the second sealing rings 37 to push the fixed rings 36 downward, which in turn can drive the first spring 38 to deform. The reaction force generated by the deformation of the first spring 38 Force can push the second sealing ring 37 into tight contact with the first sealing ring 33, which can prevent air leakage, improve sealing performance, block gas exchange between different culture wells 11 on the same culture dish, and prevent the phenomenon of one culture well 11 becoming infected and spreading to multiple surrounding culture wells 11. The bottom of the slide rod 35 has a limiting groove 61, and the bottom of the slide groove 34 is fixedly connected to a limiting rod 62. The limiting rod 62 is slidably connected to the limiting groove 61. The vertical movement of the slide rod 35 can make the limiting rod 62 slide within the limiting groove 61, which can limit the vertical movement distance of the slide rod 35 and prevent the first spring 38 from being damaged due to excessive deformation. A third sealing ring 81 is fixedly connected to one side of the bottom of the fixing ring 36. The third sealing ring 81 can provide a secondary seal between the fixing ring 36 and the culture well 11, which can further prevent gas exchange between different culture wells 11 and further prevent the phenomenon of one culture well 11 becoming infected and spreading to multiple surrounding culture wells 11.

[0019] Working principle: During operation, pulling the handle 44 moves the moving rod 42 and the locking rod 45. The movement of the moving rod 42 causes the second spring 43 to deform. Then, the fixing cover 12 is placed on top of the culture dish body 1, and the locking rod 45 aligns with the locking groove 46. The second spring 43 returns to its original position, causing the locking rod 45 to engage in the locking groove 46, thus fixing the fixing cover 12 to the culture dish body 1. While the fixing cover 12 is fixed, the pressure ring 32 can be inserted into the sealing groove 31, causing the first sealing ring 33 to contact the second sealing ring 37 and compressing the second sealing ring 37, which in turn causes the fixing ring 36 to move downward, so that the bottom of the third sealing ring 81 contacts the bottom of the sealing groove 31. At the same time, the first spring 38 deforms, utilizing the reaction force generated by the deformation of the first spring 38... The force ensures that the second sealing ring 37 and the first sealing ring 33 are in close contact, preventing gas exchange. When medication needs to be injected, the actuating plate 52 can be rotated. Rotating the actuating plate 52 will cause the sealing cap 24 to rotate to one side. Then, the syringe can be inserted into the injection channel 13 for injection. After being filtered by the bacterial filter membrane 71, the solution enters the culture well 11. After injection, the syringe is removed, and then the sealing cap 24 is rotated in the opposite direction to reset it. The first magnet 27 and the second magnet 28 are attracted and fixed, preventing the sealing cap 24 from rotating. The above is the working process of the entire device. Any content not described in detail in this specification belongs to the prior art known to those skilled in the art.

[0020] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A special culture dish for observing the dynamic growth of brain organoids, comprising a culture dish body (1), characterized in that: The top of the culture dish body (1) is provided with a plurality of culture holes (11) evenly arranged. The top of the culture dish body (1) is provided with a fixed cover (12). The top of the fixed cover (12) is fixedly connected with a plurality of liquid injection channels (13) evenly arranged. The top of the liquid injection channels (13) is provided with a sealing component. A sealing component is provided between the fixed cover (12) and the culture dish body (1). The sealing component includes a first support plate (21) fixedly connected to one side of the injection channel (13), a rotating shaft (22) fixedly connected to the top of the first support plate (21), a second support plate (23) connected to the outside of the rotating shaft (22), a sealing cover (24) fixedly connected to one side of the second support plate (23), a first mounting groove (25) opened at the bottom of the sealing cover (24), a second mounting groove (26) opened at the top side of the injection channel (13), a first magnet (27) fixedly connected to the inner wall of the first mounting groove (25), and a second magnet (28) fixedly connected to the inner wall of the second mounting groove (26). The rotating shaft (22) is rotatably connected to the second support plate (23).

2. The special culture dish for observing the dynamic growth of brain organoids according to claim 1, characterized in that: The sealing component includes multiple sealing grooves (31) evenly arranged on the top of the petri dish body (1), multiple pressure rings (32) evenly arranged on the bottom of the fixed cover (12), a first sealing ring (33) fixedly connected to the bottom of the pressure ring (32), multiple sliding grooves (34) arranged in a circular pattern on the bottom of the sealing groove (31), a sliding rod (35) connected to the sliding groove (34), a fixed ring (36) fixedly connected to the top of the sliding rod (35), a second sealing ring (37) fixedly connected to the top of the fixed ring (36), and a first spring (38) sleeved on the outside of the sliding rod (35). The pressure ring (32) is inserted into the sealing groove (31), and the two ends of the first spring (38) are fixedly connected to the bottom of the sealing groove (31) and the bottom of the fixed ring (36), respectively.

3. The special culture dish for observing the dynamic growth of brain organoids according to claim 1, characterized in that: The fixed cover (12) has a movable groove (41) on both sides. A movable rod (42) is slidably connected in the movable groove (41). A second spring (43) is sleeved on the outside of the movable rod (42). A grip plate (44) is fixedly connected to one end of the movable rod (42). A locking rod (45) is fixedly connected to one side of the grip plate (44). A locking groove (46) is opened on both sides of the petri dish body (1). The locking rod (45) is inserted into the locking groove (46). The movable rod (42) is slidably connected to the movable groove (41).

4. A special culture dish for observing the dynamic growth of brain organoids according to claim 1, characterized in that: The top of the closed cover (24) is fixedly connected to a lever (51), and the top of the lever (51) is fixedly connected to a lever plate (52).

5. A special culture dish for observing the dynamic growth of brain organoids according to claim 2, characterized in that: The bottom of the slide bar (35) has a limiting groove (61), and the bottom of the slide groove (34) is fixedly connected to a limiting rod (62), and the limiting rod (62) is slidably connected to the limiting groove (61).

6. A special culture dish for observing the dynamic growth of brain organoids according to claim 1, characterized in that: A bacterial filter membrane (71) is fixedly connected to the inner wall of the injection channel (13).

7. A special culture dish for observing the dynamic growth of brain organoids according to claim 2, characterized in that: A third sealing ring (81) is fixedly connected to one side of the bottom of the fixing ring (36).

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