A trehalose-containing cryo-recovery integrated cell culture medium

Abstract

The utility model discloses a kind of cryopreservation recovery integration cell culture medium containing trehalose, including petri dish, the petri dish one end is placed with cover body, the cover body one end is equipped with multiple groups of hole body one, the petri dish one end is connected with multiple groups of column, the cover body one end is equipped with multiple groups of hole body two.The utility model cover body multiple groups of hole body one can cooperate dropper drop different dose nutrient solution, reagent, so that the petri dish forms multiple concentration gradient culture medium environment, same sample same condition can be synchronous detection different concentration cell cryopreservation recovery effect, without multiple sets of device, reduce operation step and time cost, adapt multiple concentration comparison experiment.And column asymmetric distribution, limit cover body only assembly direction, fixed droplet area, avoid misplacement deviation, guarantee experiment initial condition uniform, improve data repeatability and reliability.

Description

Technical Field

[0001] This invention relates to the field of culture medium technology, and in particular to an integrated cryopreservation and thawing cell culture medium containing trehalose. Background Technology

[0002] Trehalose-containing cryopreservation and thawing integrated cell culture medium is a special culture medium that uses trehalose as the core protective component and can simultaneously meet the needs of cell cryopreservation and thawing culture. Its core advantages lie in simplifying the operation process and improving cell safety and thawing activity, and it has important application value in the fields of biotechnology and cell therapy.

[0003] In cell biology experiments, when it is necessary to systematically detect the effects of different concentrations of reagents, such as nutrient solutions, cryoprotectants, and regulatory factors, on cell viability, resuscitation efficiency, and functional status in trehalose-containing cryopreservation and resuscitation integrated cell culture media, the lack of dedicated devices that can simultaneously construct multiple concentration gradients within a single culture system presents significant challenges. Researchers must prepare multiple independent culture dishes, covers, and consumables based on the predetermined number of concentration gradients, and each device must be sterilized individually to avoid cross-contamination. During the experimental procedure, each independent culture device requires separate dispensing of culture medium and cell seeding. Then, reagents are precisely added to the corresponding device at the set concentration using external droppers or pipetting equipment. The correspondence between device numbers and concentration gradients must be repeatedly checked to prevent operational confusion. This process prolongs the experimental cycle, increases the complexity of the experimental operation, and raises the uncertainty of the results, making it difficult to meet the needs of efficient and precise cell cryopreservation and resuscitation concentration optimization experiments. Utility Model Content

[0004] The purpose of this invention is to address the shortcomings of existing technologies by proposing an integrated cryopreservation and resuscitation cell culture medium containing trehalose.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: A trehalose-containing cryopreservation and resuscitation integrated cell culture medium includes a culture dish, a cover is attached to one end of the culture dish, a plurality of sets of wells are formed at one end of the cover, a plurality of columns are connected to one end of the culture dish, a plurality of sets of wells are formed at one end of the cover, the plurality of sets of wells are movably connected to the corresponding columns, and a plate is attached to one end of the cover.

[0006] Preferably, the cover has grooves at one end and the other end, and the plate has blocks at one end and the other end, with the two sets of grooves respectively inserted into and engaged with the corresponding blocks.

[0007] Preferably, the outer walls of the multiple sets of columns are fitted with rubber sleeves, and the multiple sets of rubber sleeves are respectively interference-fitted with the corresponding holes.

[0008] Preferably, multiple sets of the columns are asymmetrically distributed on the culture dish.

[0009] Preferably, a rubber pad is adhered to the joint between the plate and the cover, and the rubber pad is in contact with the multiple sets of holes.

[0010] Preferably, a handle is connected to one end and the other end of the cover.

[0011] Preferably, both sets of grooves are dovetail-shaped and fit the block.

[0012] The beneficial effects of this utility model are as follows: 1. Through the coordinated design of the cap, wells, and columns, the multiple wells in the cap can be individually connected to external droppers to add different doses of nutrient solution and reagents, creating multiple concentration gradient culture medium environments within the culture dish. This design allows for simultaneous detection of cell cryopreservation and resuscitation effects under the same sample and culture conditions, eliminating the need for multiple culture devices, reducing experimental steps and time costs, and covering multi-concentration comparison experiments. Furthermore, the asymmetrical distribution of the multiple columns ensures that the cap is only assembled with the culture dish in a single correct orientation. This structure fixes the cap's installation position each time, ensuring a consistent dripping area in the wells and preventing dripping position deviations caused by cap misalignment. This effectively guarantees uniformity in the initial conditions of culture medium addition and cell contact with reagents across multiple experiments, improving the repeatability and reliability of experimental data to a certain extent. 2. Through the coordinated design of the plate, rubber pad, and culture dish, when a sealed culture environment is required, the plate adheres to the cover, and the attached rubber pad covers and closely contacts multiple wells, effectively preventing dust and microorganisms from entering the culture dish and ensuring the purity of the culture medium and cell viability. When reagents need to be added, only the plate needs to be removed to operate through the wells, without disassembling the entire cover, simplifying the operation process and reducing the risk of exposure to the culture environment. The rubber pad at the contact point between the plate and the cover not only seals the wells but also fills the tiny gaps between the plate and the cover with its elastic material, further enhancing the sealing effect. During cell cryopreservation, it can reduce the evaporation of water from the culture medium in the dish and maintain the stable concentration of protective components such as trehalose. During the thawing culture stage, it can isolate external contaminants, providing a clean and stable growth environment for cells, reducing the risk of decreased cell viability due to environmental interference, and indirectly ensuring the core efficacy of the integrated trehalose cryopreservation and thawing culture medium. Attached Figure Description

[0013] Figure 1 This is a top view schematic diagram of an integrated cryopreservation and resuscitation cell culture medium containing trehalose proposed in this utility model; Figure 2 for Figure 1 A schematic diagram of the structure without the cover installed; Figure 3 for Figure 1 A schematic diagram of the structure without the installed panels; Figure 4 for Figure 3 A cross-sectional structural diagram of the middle cover, the hole body 1, and the handle; Figure 5 for Figure 4 Schematic diagram of the structure of the middle channel, hole body one, and hole body two.

[0014] In the diagram: 1. Petri dish; 2. Cover; 3. Hole 1; 4. Column; 5. Hole 2; 6. Rubber sleeve; 7. Tank; 8. Block; 9. Plate; 10. Rubber pad; 11. Handle. Detailed Implementation

[0015] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0016] Example 1, referring to Figures 1 to 5 A trehalose-containing cryopreservation and resuscitation integrated cell culture medium includes a culture dish 1 for storing or culturing trehalose-containing cryopreservation and resuscitation integrated cell culture medium. A cover 2 is attached to one end of the culture dish 1. Multiple sets of wells 3 are formed at one end of the cover 2. The wells 3 can be used with an external dropper to drip nutrient solution or other reagents into the culture dish 1. Multiple sets of columns 4 are connected to one end of the culture dish 1. Multiple sets of wells 5 are formed at one end of the cover 2 to guide the installation of the cover 2. The multiple sets of wells 5 are movably connected to the corresponding columns 4. A plate 9 is attached to one end of the cover 2. The arrangement of multiple sets of wells 3 allows different amounts of nutrient solution or other reagents to be dripped into each well 3, resulting in different concentrations in different parts of the culture dish 1. This allows for the detection of different concentrations of the same sample and has a wide range of applicability to a certain extent.

[0017] In this embodiment, grooves 7 are provided at one and the other ends of the cover 2, and blocks 8 are connected to one and the other ends of the plate 9. The two sets of grooves 7 are inserted into the corresponding blocks 8. Inserting one end of the plate 9 into the groove 7 seals the hole 3, preventing dust from entering. When nutrient solution or other reagents need to be added, the cover 2 is removed, and the solution can be added through the hole 3, making it relatively convenient to use. Rubber sleeves 6 are fitted onto the outer walls of multiple sets of columns 4, and these rubber sleeves 6 are respectively press-fitted into the corresponding holes 5, fixing the cover 2 in place. The multiple sets of columns 4 are asymmetrically distributed on the petri dish 1, and the cover 2 can only be inserted in the correct direction, ensuring that the area where nutrient solution drips is consistent in each experiment. A rubber pad 10 is adhered to the joint between the plate 9 and the cover 2, and the rubber pad 10 contacts the multiple sets of holes 3, improving sealing and preventing dust from entering. Handles 11 are connected to one and the other ends of the cover 2, making it easier to move the cover 2. Both sets of grooves 7 are dovetail-shaped and fit into the block 8, increasing friction and providing horizontal constraint.

[0018] The working principle of this embodiment is as follows: During use, the cover 2 is assembled with the corresponding column 4 on the culture dish 1 through the second set of holes 5. Because the multiple sets of columns 4 are asymmetrically distributed, the cover 2 can only be installed in one correct direction, ensuring that the first hole 3 on the cover 2 is always aligned with the preset reagent addition area inside the culture dish 1. Simultaneously, the rubber sleeve 6 on the outer wall of the column 4 forms an interference fit with the second set of holes 5, which stably fixes the cover 2 on the culture dish 1, preventing displacement after assembly. After assembly, if it is necessary to conduct cell cryopreservation and resuscitation effect testing with different concentration variables, the multiple sets of first holes 3 on the cover 2 can be used directly to add different doses of nutrient solution or experimental reagents to the culture dish 1 by matching the external dropper with the corresponding first hole 3. By controlling the amount added to each set of first holes 3, multiple concentration gradient trehalose-containing culture medium environments are formed inside the culture dish 1, meeting the needs of multivariate comparative experiments under the same sample and culture conditions. When no reagents need to be added and the internal environment of culture dish 1 needs to be maintained, insert the block 8 at one end of plate 9 into the corresponding dovetail groove 7 on cover 2, ensuring that plate 9 fits tightly against cover 2. At this time, the rubber pad 10 at the contact point between plate 9 and cover 2 will completely cover and closely contact the multiple sets of wells 3. This prevents external dust and microorganisms from entering culture dish 1, ensuring the purity of the culture medium and cell viability; it also reduces water evaporation from the culture medium in culture dish 1, maintaining a stable concentration of protective components such as trehalose, and meeting the environmental requirements of cell cryopreservation. During cell resuscitation, if nutrient solution or experimental reagents need to be added, it is not necessary to disassemble the entire cover 2. Simply pull plate 9 gently using the handles 11 at both ends of cover 2 to detach block 8 from groove 7 and remove plate 9. Reagents can then be added again through wells 3. After the operation is complete, reinstall plate 9 to quickly restore the closed state of the culture environment, reducing the risk of exposure to the culture environment and providing clean and stable conditions for cell resuscitation and growth, ensuring the core function of the integrated trehalose cryopreservation and resuscitation culture medium. After the experiment, by pulling the cover 2 upward by the handle 11, the second hole 5 can be separated from the column 4, thus separating the cover 2 from the culture dish 1. Then, the plate 9 is removed, and the culture dish 1, cover 2, plate 9 and rubber pad 10 are cleaned respectively for subsequent reuse.

[0019] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A cryopreservation and resuscitation integrated cell culture medium containing trehalose, comprising a culture dish (1), characterized in that, The petri dish (1) has a cover (2) attached to one end. The cover (2) has multiple sets of holes (3) at one end. The petri dish (1) is connected to multiple sets of columns (4) at one end. The cover (2) has multiple sets of holes (5) at one end. The multiple sets of holes (5) are connected to the corresponding columns (4) and a plate (9) is attached to one end of the cover (2).

2. The trehalose-containing cryopreservation and resuscitation integrated cell culture medium according to claim 1, characterized in that, The cover (2) has grooves (7) at one end and the other end, and the plate (9) has blocks (8) at one end and the other end. The two sets of grooves (7) are inserted into the corresponding blocks (8).

3. The trehalose-containing cryopreservation and resuscitation integrated cell culture medium according to claim 1, characterized in that, The outer walls of the multiple sets of columns (4) are fitted with rubber sleeves (6), and the multiple sets of rubber sleeves (6) are respectively interference-fitted with the corresponding holes (5).

4. The trehalose-containing cryopreservation and resuscitation integrated cell culture medium according to claim 1, characterized in that, Multiple sets of the columns (4) are asymmetrically distributed on the petri dish (1).

5. The trehalose-containing cryopreservation and resuscitation integrated cell culture medium according to claim 1, characterized in that, A rubber pad (10) is adhered to the joint between the plate (9) and the cover (2), and the rubber pad (10) is in contact with the multiple sets of holes (3).

6. The trehalose-containing cryopreservation and resuscitation integrated cell culture medium according to claim 1, characterized in that, The cover (2) is connected to a handle (11) at one end and the other end.

7. The trehalose-containing cryopreservation and resuscitation integrated cell culture medium according to claim 2, characterized in that, Both sets of grooves (7) are dovetail-shaped and fit into the block (8).

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