A cell culture monitoring device

Abstract

The utility model relates to cell culture technical field discloses a cell culture monitoring devices, include: bearing assembly, including bracket and for bearing the bearing mouth of culture dish, bearing mouth is located on the bracket, light source component is set in the top of bearing assembly with bearing mouth opposite, and imaging component is set in the below of bearing assembly, imaging component includes objective lens module, right angle mirror module, adjustable lens barrel and camera connected in proper order, wherein, adjustable lens barrel is located in the side close to the bracket, and the objective lens of objective lens module points to bearing mouth, adjustable lens barrel is used for adjusting the distance between camera and right angle mirror module to switch imaging magnification times.

Description

Technical Field

[0001] This utility model belongs to the field of cell culture technology, and specifically relates to a cell culture monitoring device. Background Technology

[0002] When observing cell states using a traditional cell microscope, it is usually necessary to remove the culture dish containing the cells from the incubator and place it under the microscope for observation. This operation exposes the cell sample from the high-cleanliness incubator environment to a lower-cleanliness external environment, significantly increasing the risk of contamination. In addition, prolonged exposure of cells to constant temperature and pressure culture conditions can easily affect their physiological state, leading to a decline in their condition. Therefore, traditional microscopes are not suitable for long-term, real-time observation of cells.

[0003] Currently, the magnification adjustment of cell monitors largely relies on adjusting the combination of the distance between the camera and the sample, as well as the objective and eyepiece magnification. This results in limited overall magnification and a tendency for image distortion. When using high-power objectives, the camera lens must be kept extremely close to the objective to avoid collisions with the slide, leaving little room for error. Furthermore, the objective turrets in existing monitors are relatively large, leading to a bulky overall size and occupying significant space within the incubator, thus limiting their application in high-density culture environments. Utility Model Content

[0004] To address the shortcomings of the prior art, this invention provides a cell culture monitoring device that enables the overall monitoring device to be miniaturized, with each component working together to form a compact layout.

[0005] The technical effects to be achieved by this utility model are realized through the following aspects: This utility model provides a cell culture monitoring device, comprising: The support assembly includes a bracket and a support port for holding a culture dish, the support port being disposed on the bracket; A light source assembly is disposed above the carrier assembly and opposite the carrier port; and An imaging component is disposed below the supporting component, and the imaging component includes an objective lens module, a right-angle lens module, an adjustable lens barrel, and a camera connected in sequence. The adjustable lens barrel is located on the side near the bracket, and the objective lens of the objective lens module points towards the receiving port; the adjustable lens barrel is used to adjust the distance between the camera and the right-angle lens module to switch the imaging magnification.

[0006] In some implementations, the adjustable lens barrel is provided with a sleeve lens, which is located on the side close to the right-angle lens module.

[0007] In some implementations, the adjustable lens barrel further includes an extension tube, which forms a retractable nested structure inside the adjustable lens barrel; the extension tube is mounted on the adjustable lens barrel at one end near the sleeve lens.

[0008] In some implementations, the imaging assembly further includes two support seats for limiting and fixing, the two support seats being respectively sleeved on the outside of the adjustable lens tube and the outside of the extension tube.

[0009] In some implementations, the support base is provided with a limiting screw hole for fixing the adjusted optical path length of the adjustable lens barrel.

[0010] In some implementations, the support component further includes an adjustment mechanism connected to the bracket for driving the bracket to move longitudinally, laterally, and vertically.

[0011] In some implementations, the support assembly further includes a first adjusting member, a second adjusting member, and a third adjusting member connected to the adjusting mechanism, which are used to adjust the bracket to move longitudinally, laterally, and vertically, respectively.

[0012] In some implementations, a housing assembly is also included, which includes a base and a housing. The housing is fitted over the outside of the base, and the support assembly and the imaging assembly are both located inside the housing and mounted on the base. The outer shell is provided with adjustment holes for the first adjustment member, the second adjustment member and the third adjustment member to extend out of the shell assembly, and the outer shell is also provided with a hollow opening for the petri dish to extend out of the shell assembly.

[0013] In some implementations, the right-angle lens module includes a reflector for deflecting light from the objective lens module before it is received by the camera via the adjustable lens barrel.

[0014] In some implementations, the camera includes a wireless transmission unit for sending image-acquired data to an external device.

[0015] In summary, this utility model has at least the following advantages: This utility model provides a cell culture monitoring device with a cavity design where the outer shell and base are connected. This integrates the support components, light source components, and imaging components into a single space, avoiding the bulkiness of the device caused by scattered components. This allows for cell culture and monitoring within the limited space of the incubator. The imaging component consists of an objective lens module, a right-angle lens module, an adjustable lens barrel, and a camera. The right-angle lens module redirects the light path, and the adjustable lens barrel allows for adjusting the distance between the camera and the right-angle lens module to switch magnifications. This eliminates the need for multiple independent objective lens modules with fixed focal lengths, reducing the number of components and space occupied, resulting in a more compact monitoring device. The components work together to form a compact layout. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of the cell culture monitoring device provided in an embodiment of the present invention.

[0017] Figure 2 A schematic diagram of the internal structure of the cell culture monitoring device provided in this embodiment of the present invention.

[0018] Figure 3 This is a schematic diagram of the imaging component provided in an embodiment of the present invention.

[0019] Marked in the image: 1. Housing assembly; 11. Outer shell; 111. Hollowed-out opening; 112. Adjustment hole; 12. Base; 2. Support assembly; 21. Adjustment mechanism; 22. First adjustment component; 23. Second adjustment component; 24. Third adjustment component; 25. Bracket; 251. Support port; 26. Petri dish; 3. Light source assembly; 31. Light source support frame; 32. LED light source; 4. Imaging assembly; 41. Objective lens module; 411. Objective lens; 412. Mounting base; 42. Right angle mirror module; 43. Adjustable lens barrel; 431. Sleeve lens; 432. Extension tube lens; 44. Camera; 441. Wireless transmission unit; 45. Support base; 451. Limiting screw hole. Detailed Implementation

[0020] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. The described embodiments are some, but not all, of the embodiments of this utility model.

[0021] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0022] Example 1: Please see Figure 1 and Figure 2 and combined Figure 3 This utility model discloses a cell culture monitoring device, comprising: a support component 2, including a bracket 25 and a support port 251 for supporting a culture dish 26, the support port 251 being disposed on the bracket 25; a light source component 3, disposed above the support component 2 and opposite to the support port 251; and an imaging component 4, disposed below the support component 2, the imaging component 4 including an objective lens module 41, a right-angle lens module 42, an adjustable lens barrel 43, and a camera 44 connected in sequence; wherein, the adjustable lens barrel 43 is disposed on the side near the bracket 25, and the objective lens 411 of the objective lens module 41 points towards the support port 251; the adjustable lens barrel 43 is used to adjust the distance between the camera 44 and the right-angle lens module 42 to switch the imaging magnification.

[0023] Specifically, the device also includes a housing assembly 1, which is formed by connecting a base 12 and an outer shell 11. The two are assembled to form a cavity, providing a stable internal environment for each component and serving a protective function. Inside the cavity, i.e., on the base 12, are mounted a support assembly 2, a light source assembly 3, and an imaging assembly 4. The support assembly 2 is provided with a bracket 25 for supporting a culture dish 26. The bracket 25 is provided with a support opening 251 for placing the culture dish 26. The support opening 251 may optionally include, but is not limited to, a hollow structure and a limiting block. The hollow structure facilitates the observation of cells in the culture dish 26 using the objective lens module 41, while the limiting block can adapt to the corresponding size of the culture dish 26, so that it can be placed stably and provide positioning and fixation assistance.

[0024] The light source assembly 3 is fixed on the base 12, and its light path exit position is set directly above the support assembly 2, so that the light can illuminate the culture dish 26 and provide bright field illumination for the imaging assembly 4 set below the support assembly 2. The culture dish 26 is made of transparent material so that the imaging assembly 4 can collect image data of the cells in the culture dish 26 through the culture dish 26. The imaging assembly 4 includes an objective lens module 41, a right-angle lens module 42, an adjustable lens tube 43, and a camera 44 arranged sequentially along the light path. The adjustable lens tube 43 can adjust the distance of the light path by extending and retracting, thereby changing the imaging focal length and thus changing the magnification of the overall imaging assembly 4, realizing multi-level magnification adjustment for cell monitoring.

[0025] Understandably, all components of the cell culture monitoring device are built inside the shell component 1. The device is placed in the cell culture chamber and, through the imaging component 4 and the carrier component 2, the cell images of the cells in the culture dish 26 are acquired and transmitted, achieving isolated monitoring without interfering with the cell culture environment.

[0026] It is worth noting that the objective lens 411 of the objective lens module 41 points towards the carrier port 251 to receive the transmitted light from the culture dish 26 after passing through the cells. The imaging component 4 then uses the right-angle lens module 42 to deflect the light path, converting the vertically extending light path in a traditional microscope into a horizontal or specific angle extension. This avoids the imaging component 4 occupying too much space in the vertical direction. The deflected layout makes the overall structure of the device compact, with higher space utilization, and improves operability within the limited space of the incubator. The magnification of the imaging component 4 is adjusted using an adjustable tube 43, replacing the previous method of mechanically switching between different fixed magnification objective lenses 411 to adjust the imaging magnification. This achieves stepless magnification change and avoids the use of a turntable for switching objective lenses 411, which would occupy a large amount of internal space in the incubator.

[0027] In this embodiment, the light emitted by the light source assembly 3 illuminates the cells in the culture dish 26 located on the holder 25. The transmitted light after passing through the cells passes through the culture dish 26 and enters the objective lens module 41. After initial magnification, it is transmitted to the right-angle lens module 42, where the horizontal light path is converted into a vertical light path. The light is then transmitted to the camera 44 via the adjustable lens tube 43. The camera 44 completes image acquisition and transmission, allowing researchers to monitor the cells outside the incubator. The imaging assembly 4 combines the light path conversion of the right-angle lens module 42 with the continuous magnification of the adjustable lens tube 43 to form a spatially efficient and precisely controlled imaging system, thereby reducing the space occupied by the device.

[0028] Example 2: This embodiment represents a further structural optimization of the cell culture monitoring device of this invention. Please refer to [link / reference]. Figure 2 and 3 .

[0029] In some embodiments, the adjustable lens barrel 43 is provided with a sleeve lens 431, which is located on the side close to the right-angle lens module 42.

[0030] Specifically, since the adjustable lens barrel 43 needs to extend and retract within a certain range to switch the magnification, and the change in the optical path length will cause the aberration parameters to fluctuate dynamically, the sleeve lens 431 is set between the right-angle lens module 42 and the adjustable lens barrel 43 to correct the propagation path of light of different wavelengths and avoid problems such as blurred image edges and color shift caused by aberration.

[0031] In some embodiments, the adjustable lens barrel 43 is further provided with an extension tube lens 432, which forms a retractable nested structure inside the adjustable lens barrel 43; the extension tube lens 432 is installed at one end of the adjustable lens barrel 43 near the sleeve lens 431.

[0032] Specifically, the adjustable lens barrel 43 achieves its telescopic adjustment function through the extension tube 432. The extension tube 432 has a nested structure, allowing the adjustable lens barrel 43 to slide axially along the outer wall of the extension tube 432. This ensures coaxiality of the optical path while adjusting the optical path transmission length according to the amount of telescopic extension. The extension tube 432 is located at one end of the adjustable lens barrel 43, in the optical path between the sleeve lens 431 and the camera 44, enabling axial telescopic movement of the adjustable lens barrel 43. The movement of the extension tube 432 can be selected, but is not limited to, through a manual fine-tuning knob or an electric drive connected to the control unit, thereby achieving precise telescopic control and realizing continuous and stepless magnification changes.

[0033] It is worth noting that the use of the extension tube 432 to achieve magnification avoids the mechanical vibration and positioning errors caused by the rotation of the traditional objective turret. This also prevents the target cells from being lost in the field of view when switching magnifications during monitoring. This is crucial for cell culture experiments that require long-term stable observation and can effectively ensure the consistency and accuracy of monitoring data.

[0034] In some embodiments, the imaging assembly 4 further includes two support seats 45 for limiting and fixing, the two support seats 45 being respectively sleeved on the outside of the adjustable lens tube 43 and the outside of the extension tube 432.

[0035] Specifically, two support seats 45 are provided along the length of the adjustable lens barrel 43. The support seats 45 are fixedly connected to the outer side of the adjustable lens barrel 43. The two support seats 45 are respectively sleeved on the adjustable lens barrel 43 and the extension tube lens 432, and fixed to the base 12 to limit the radial and axial movement of the adjustable lens barrel 43, ensuring that the adjustable lens barrel 43 always maintains coaxiality with the support component 2 and the camera 44 when it extends or retracts.

[0036] Furthermore, the support base 45 is provided with a limiting screw hole 451 for fixing the adjusted optical path length of the adjustable lens barrel 43. A sealing ring is provided at the end of the extension tube near the camera. This sealing ring not only provides dustproof sealing inside the lens barrel but also provides uniform damping force for the extension and retraction adjustment of the adjustable lens barrel 43, ensuring smooth adjustment and accurate magnification. In some embodiments, the device can be pre-assembled, the extension and retraction of the adjustable lens barrel 43 can be adjusted, and then the outer wall of the adjustable lens barrel 43 can be fastened and locked using assembly screws through the limiting screw hole.

[0037] In some embodiments, the right-angle lens module 42 is provided with a reflector to deflect the light from the objective lens module 41 and then receive it through the adjustable lens tube 43 to the camera 44.

[0038] Specifically, a reflector is provided within the right-angle mirror module 42. This reflector ensures that the direction of light propagation after the deflection is consistent, preventing scattering or deviation, and guaranteeing the clarity and fidelity of the cell image. Optional, but not limited to, a protective lens may be included within the right-angle mirror module 42. The protective lens is sealed and installed on the opening of the right-angle mirror module 42 facing the culture dish 26.

[0039] In this embodiment, the adjustable lens barrel 43 is fixed to the base 12 by a support 45. A sleeve lens 431 is provided inside the adjustable lens barrel 43 to correct aberrations in the middle section of the imaging optical path. Between the sleeve lens 431 and the camera 44, a telescopically adjustable extension tube 432 is provided. This extension tube 432 provides a smooth magnification mode, realizing continuous and stepless magnification, and ensuring the continuity of monitoring data. The right-angle mirror module 42 bends the optical path through a reflector, ensuring the stability and reproduction of light transmission, and ensuring the accuracy of the image data acquired by the camera 44.

[0040] Example 3: This embodiment further optimizes the structure of the cell culture monitoring device based on the above embodiments. Please refer to [link / reference needed]. Figure 2 and Figure 3 and combined Figure 1 .

[0041] In some embodiments, the support component 2 further includes an adjustment mechanism 21, which is connected to the bracket 25 and is used to drive the bracket 25 to move longitudinally, laterally, and vertically.

[0042] Specifically, the bracket 25 of the support component 2 is fixedly connected to the base 12 through the adjustment mechanism 21. The adjustment mechanism 21 drives the bracket 25 to achieve precise movement in three dimensions, so that the cell samples in different regions of the culture dish 26 located on the bracket 25 can be aligned with the imaging optical path by adjusting their positions. Moreover, in the vertical direction, the distance between the culture dish 26 and the objective lens module 41 can be finely adjusted to adapt to culture dishes 26 of different thicknesses or to achieve focusing of the imaging component 4.

[0043] In some embodiments, the support assembly 2 further includes a first adjusting member 22, a second adjusting member 23 and a third adjusting member 24 connected to the adjusting mechanism 21, which are used to adjust the bracket 25 to move longitudinally, laterally and vertically, respectively.

[0044] Furthermore, the cell culture monitoring device also includes a housing assembly 1, which includes a base 12 and a shell 11. The shell 11 is fitted on the outside of the base 12, and the support assembly 2 and the imaging assembly 4 are both located inside the shell 11 and mounted on the base 12. The shell 11 is provided with adjustment holes 112 for the first adjustment member 22, the second adjustment member 23 and the third adjustment member 24 to extend out of the shell assembly 1. The shell 11 is also provided with a hollow opening 111 for the culture dish 26 to extend out of the shell assembly 1.

[0045] Specifically, the adjustment mechanism 21 is installed inside the housing assembly 1. Multiple adjustment holes 112 communicating with the interior are provided on the outer shell 11. The first adjustment member 22, the second adjustment member 23 and the third adjustment member 24 pass through the corresponding adjustment holes 112 and enter the cavity of the housing assembly 1, and are connected to the drive end of the adjustment mechanism 21 through a coupling. The first adjustment member 22 is used to drive the bracket 25 to move longitudinally, the second adjustment member 23 is used to drive the bracket 25 to move laterally, and the third adjustment member 24 is used to drive the bracket 25 to move vertically.

[0046] The first adjusting member 22 and the second adjusting member 23 are used to adjust the movement of the support 25 on the horizontal plane, so that the area to be tested in the culture dish 26 can move into the imaging optical path, allowing the cells in the area to be photographed by the camera 44. The third adjusting member 24 is used to adjust the distance between the culture dish 26 on the support 25 and the objective lens module 41, so that it can work with the adjustable lens tube 43 to achieve precise focusing of the cells. The outer shell 11 has a hollow opening 111 at the top, which is opposite to the support 25, so that the experimenter can place the culture dish 26 on the carrier port 251 on the support 25 through the hollow opening 111. In some embodiments, the light source assembly 3 includes a light source support frame 31 and an LED light source 32. The light source support frame 31 is fixedly connected to the base 12, and the LED light source 32 is mounted on the light source support frame 31 and is positioned directly opposite the petri dish 26.

[0047] Specifically, the light source support frame 31 is fixedly connected to the base 12 and extends through the outer shell 11 to the outside of the housing assembly 1. The LED light source 32 is fixedly positioned by the support frame 31 and installed above the culture dish 26 on the support assembly 2, so that the light emission center of the LED light source 32 is coaxial with the imaging center of the objective lens module 41, ensuring that the reflected light from the cells enters the imaging optical path efficiently and provides an illumination field for clear imaging. That is, the emitted beam of the LED light source 32 illuminates the culture dish 26 on the support 25 through the perforated opening 111. The LED light source 32, which is located on the outside of the housing assembly 1, is easy for experimental personnel to replace and maintain, or to replace the fluorescent light source, add filters, etc., to meet different monitoring needs. This application does not limit this.

[0048] In addition, the objective lens module 41 includes an objective lens 411, which is disposed in the output light path of the LED light source 32. The objective lens 411 can be, but is not limited to, a 10x objective lens 411. The objective lens 411 is fixed on the right-angle lens module 42 by a mounting base 412 and is coaxial with the imaging light path to ensure that the light collected by the objective lens 411 can be accurately transmitted to the right-angle lens module 42 along the preset light path.

[0049] In some embodiments, the camera 44 includes a wireless transmission unit 441 for transmitting image acquisition data to an external device.

[0050] Specifically, the wireless transmission unit 441 may be, but is not limited to, a communication module based on wireless protocols such as Wi-Fi or Bluetooth, and is connected to the image processing core board of the camera 44. It is used to transmit the raw images acquired by the camera 44 or the compressed and encoded images in the form of wireless signals, so that external devices can connect and pair, complete data transmission and remote access, and enable researchers to obtain the cell culture status anytime and anywhere without frequently entering the laboratory environment to open the incubator, reducing human interference to the culture environment.

[0051] In this embodiment, external experimenters can adjust the position of the support component 2 inside the housing assembly 1 using various adjustment components, enabling the adjustment mechanism 21 of the support component 2 to move longitudinally, laterally, and vertically, facilitating the movement of the target area to be measured to a position that the camera 44 can capture. The LED light source 32 of the light source assembly 3 is located outside the housing assembly 1, allowing experimenters to easily replace it for different monitoring scenarios. A perforated opening 111 is provided on the top of the housing assembly 1, facilitating direct observation and initial position adjustment by the operator. The perforated opening 111 also facilitates direct sampling of the culture dish 26 or replacement of the culture dish 26 to be tested. Furthermore, the camera 44 uses wireless communication technology for transmission, further ensuring the stability of its internal environment.

[0052] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0053] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product of this utility model is in use. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In addition, the terms "first," "second," and "third," etc., are only used to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0054] Furthermore, terms such as "horizontal," "vertical," and "sag" do not imply that components must be absolutely horizontal or suspended, but rather that they can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal relative to "vertical," and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0055] In this invention, unless otherwise expressly specified and limited, "above or below" the first feature may include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on" the first feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the first feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0056] Although the description of this utility model has been given in conjunction with the specific embodiments described above, it is obvious to those skilled in the art that many substitutions, modifications, and variations can be made based on the above description. Therefore, all such substitutions, modifications, and variations are included within the spirit and scope of the appended claims.

Claims

1. A cell culture monitoring device, characterized in that, include: The support assembly includes a bracket and a support port for holding a culture dish, the support port being disposed on the bracket; A light source assembly is disposed above the carrier assembly and opposite the carrier port; as well as An imaging component is disposed below the supporting component, and the imaging component includes an objective lens module, a right-angle lens module, an adjustable lens barrel, and a camera connected in sequence. The adjustable lens barrel is located on the side near the bracket, and the objective lens of the objective lens module points towards the receiving port; the adjustable lens barrel is used to adjust the distance between the camera and the right-angle lens module to switch the imaging magnification.

2. The cell culture monitoring device according to claim 1, characterized in that, The adjustable lens barrel is equipped with a sleeve lens, which is located on the side close to the right-angle lens module.

3. The cell culture monitoring device according to claim 2, characterized in that, The adjustable lens barrel is provided with an extension tube lens, which forms a retractable nested structure inside the adjustable lens barrel; the extension tube lens is installed at one end of the adjustable lens barrel near the sleeve lens.

4. The cell culture monitoring device according to claim 3, characterized in that, The imaging assembly also includes two support seats for limiting and fixing, the two support seats being respectively sleeved on the outside of the adjustable lens tube and the outside of the extension tube.

5. The cell culture monitoring device according to claim 4, characterized in that, The support base is provided with a limiting screw hole for fixing the optical path length of the adjustable lens barrel after adjustment.

6. The cell culture monitoring device according to claim 1, characterized in that, The supporting component also includes an adjustment mechanism connected to the bracket, which is used to drive the bracket to move longitudinally, laterally, and vertically.

7. The cell culture monitoring device according to claim 6, characterized in that, The supporting assembly also includes a first adjusting member, a second adjusting member, and a third adjusting member connected to the adjusting mechanism, which are used to adjust the bracket to move longitudinally, laterally, and vertically, respectively.

8. The cell culture monitoring device according to claim 7, characterized in that, It also includes a housing assembly, which includes a base and a housing, the housing being fitted over the outside of the base, and the support assembly and the imaging assembly being located inside the housing and mounted on the base; The outer shell is provided with adjustment holes for the first adjustment member, the second adjustment member and the third adjustment member to extend out of the shell assembly, and the outer shell is also provided with a hollow opening for the petri dish to extend out of the shell assembly.

9. The cell culture monitoring device according to claim 1, characterized in that, The right-angle lens module includes a reflector for deflecting light from the objective lens module before it is received by the camera through the adjustable lens barrel.

10. The cell culture monitoring device according to claim 1, characterized in that, The camera includes a wireless transmission unit for sending image acquisition data to an external device.

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