A pneumatic ATF filling device

Abstract

The utility model relates to biological reactor technical field, concretely relates to a kind of pneumatic ATF perfusion equipment, including power structure, air suction member, air blowing member, three-way electromagnetic valve, buffer, control member, first connecting piece, hollow fiber column, second connecting piece and reactor, power structure provides the power of air suction member or air blowing member, power structure pre-ventilation, the liquid in reactor is inhaled to hollow fiber column and then inhaled to buffer, when liquid level reaches certain liquid level, control member controls corresponding air suction member and air blowing member to realize blowing and suction, then the repeated suction and blowing action of liquid is realized by the PLC control of setting, to reach ATF perfusion, also will not cause shear to cell, also will not contaminate cell, solve the existing ATF perfusion equipment is peristaltic pump and piston pump to do repeated motion, so it can cause the problem of large equipment use area and cumbersome operation.

Description

Technical Field

[0001] This utility model relates to the field of bioreactor technology, and in particular to a pneumatic ATF irrigation device. Background Technology

[0002] ATF perfusion equipment is designed for high-density continuous cell culture and is widely used in the biopharmaceutical field, such as the production of monoclonal antibodies, viral vaccines and gene therapy products. The alternating tangential flow (ATF) system, designed based on the hollow fiber column retention principle, has the effects of relieving filter membrane clogging and low shear force, making it a superior cell retention device.

[0003] Currently, existing ATF irrigation equipment uses peristaltic pumps and piston pumps to perform repetitive movements, which results in a large equipment area and cumbersome operation. Utility Model Content

[0004] The purpose of this invention is to provide a pneumatic ATF irrigation device, which aims to solve the problem that existing ATF irrigation devices use peristaltic pumps and piston pumps to make repeated movements, resulting in large equipment usage areas and cumbersome operation.

[0005] To achieve the above objectives, this utility model provides a pneumatic ATF perfusion device, including a power structure, an air intake component, an air blowing component, a three-way solenoid valve, a buffer component, a control component, a first connecting component, a hollow fiber column, a second connecting component, and a reactor;

[0006] The suction component is disposed on one side of the power structure; the blowing component is disposed on one side of the power structure; the three-way solenoid valve is connected to the suction component and the blowing component respectively, and is located on one side of the suction component and the blowing component; the buffer component is disposed on one side of the three-way solenoid valve; the control component is disposed on one side of the buffer component; the first connecting component is disposed on one side of the buffer component; the hollow fiber column is connected to the first connecting component and is located on one side of the first connecting component; the second connecting component is disposed on one side of the hollow fiber column; the reactor is connected to the second connecting component and is located on one side of the second connecting component.

[0007] The power structure includes a clean compressed air receiver, a pressure reducing valve, and a vacuum generator. The pressure reducing valve is connected to the clean compressed air receiver and is located on one side of the clean compressed air receiver. The vacuum generator is located on one side of the pressure reducing valve.

[0008] The vacuum generating component includes a vacuum generator and a first filter. The vacuum generator is connected to the pressure reducing valve and is located on one side of the pressure reducing valve. The first filter is connected to the vacuum generator and is located on one side of the vacuum generator.

[0009] The suction component includes a suction one-way valve and a suction pipe. The suction one-way valve is connected to the first filter and is located on one side of the first filter. The suction pipe is connected to the suction one-way valve and also to the three-way solenoid valve, and is located on one side of the suction one-way valve.

[0010] The air blowing component includes an air blowing one-way valve and an air blowing pipe. The air blowing one-way valve is connected to the first filter and is located on one side of the first filter. The air blowing pipe is connected to the air blowing one-way valve and also to the three-way solenoid valve, and is located on one side of the air blowing one-way valve.

[0011] The buffer component includes a motion tube, a sterilization filter, and a buffer tube. The motion tube is connected to the three-way solenoid valve and is located on one side of the three-way solenoid valve. The sterilization filter is connected to the motion tube and is located on one side of the motion tube. The buffer tube is connected to the sterilization filter and is located on one side of the sterilization filter.

[0012] The control component includes an upper liquid level switch and a lower liquid level switch. The upper liquid level switch is fixedly connected to the buffer tube and is located on one side of the buffer tube. The lower liquid level switch is fixedly connected to the buffer tube and is located on the side of the buffer tube away from the upper liquid level switch.

[0013] The first connector includes a first connecting pipe and a first solenoid valve. The first connecting pipe is connected to the buffer pipe and is located on one side of the buffer pipe. The first solenoid valve is connected to the first connecting pipe and to the hollow fiber column, and is located on one side of the first connecting pipe.

[0014] The second connector includes a second connecting pipe and a second solenoid valve. The second connecting pipe is connected to the buffer pipe and is located on one side of the hollow fiber column. The second solenoid valve is connected to the second connecting pipe and the reactor, and is located on one side of the second connecting pipe.

[0015] This utility model discloses a pneumatic ATF perfusion device. During use, the power structure provides power to the suction or blowing component. The power structure is pre-ventilated to draw liquid from the reactor into the hollow fiber column and then into the buffer. When the liquid level reaches a certain level, the control component controls the corresponding suction and blowing components to achieve blowing and suction. Then, the liquid is repeatedly suctioned and blown by a set PLC control, thereby achieving ATF perfusion without shearing or contaminating the cells. This solves the problem of existing ATF perfusion devices using peristaltic pumps and piston pumps for repeated movements, which results in large equipment area and cumbersome operation. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below.

[0017] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0018] 101-Power structure, 102-Inhalation component, 103-Blowing component, 104-Three-way solenoid valve, 105-Buffer component, 106-Control component, 107-First connecting component, 108-Hollow fiber column, 109-Second connecting component, 110-Reactor, 111-Clean compressed air receiver, 112-Pressure reducing valve, 113-Vacuum generator, 114-Vacuum generator, 115-First filter, 116-Inhalation check valve, 117-Inhalation pipe, 118-Blowing check valve, 119-Blowing pipe, 120-Motion pipe, 121-Sterilization filter, 122-Buffer pipe, 123-Upper liquid level switch, 124-Lower liquid level switch, 125-First connecting pipe, 126-First solenoid valve, 127-Second connecting pipe, 128-Second solenoid valve. Detailed Implementation

[0019] The embodiments of the present invention are described in detail below. Examples of the embodiments are shown in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the present invention, but should not be construed as limiting the present invention.

[0020] Please see Figure 1 ,in, Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0021] This utility model discloses a pneumatic ATF perfusion device, comprising a power structure 101, an air intake component 102, an air blowing component 103, a three-way solenoid valve 104, a buffer component 105, a control component 106, a first connecting component 107, a hollow fiber column 108, a second connecting component 109, and a reactor 110. The power structure 101 includes a clean compressed air receiver 111, a pressure reducing valve 112, and a vacuum generator 113. The vacuum generator 113 includes a vacuum generator 114 and a first filter 115. The air intake component 102 includes an air intake one-way valve 116 and an air intake pipe 117. The air blowing component... The air component 103 includes a one-way air blowing valve 118 and an air blowing pipe 119. The buffer component 105 includes a motion pipe 120, a sterilization filter 121, and a buffer pipe 122. The control component 106 includes an upper liquid level switch 123 and a lower liquid level switch 124. The first connecting component 107 includes a first connecting pipe 125 and a first solenoid valve 126. The second connecting component 109 includes a second connecting pipe 127 and a second solenoid valve 128. The aforementioned solution solves the problem that existing ATF perfusion equipment uses peristaltic pumps and piston pumps for repetitive motion, which results in a large equipment area and cumbersome operation.

[0022] In this specific embodiment, the main body of the irrigation device and the working component device can be integrated into a 316L stainless steel box (approximately 45×45cm in size). The system connects the gas source and the reactor via quick connectors to achieve rapid disassembly and sterilization.

[0023] The suction component 102 is disposed on one side of the power structure 101; the blowing component 103 is disposed on one side of the power structure 101; the three-way solenoid valve 104 is connected to the suction component 102 and the blowing component 103 respectively, and is located on one side of the suction component 102 and the blowing component 103; the buffer component 105 is disposed on one side of the three-way solenoid valve 104; the control component 106 is disposed on one side of the buffer component 105; the first connecting component 107 is disposed on one side of the buffer component 105; the hollow fiber column 108 is connected to the first connecting component 107 and is located on one side of the first connecting component 107; the second connecting component 109 is disposed on one side of the hollow fiber column 108; the reactor 110 is connected to the second connecting component 109. 9 is connected and located on one side of the second connector 109. In use, the power structure 101 provides power to the suction component 102 or the blowing component 103. The power structure 101 is ventilated in advance to draw the liquid in the reactor 110 into the hollow fiber column and then into the buffer component 105. When the liquid level reaches a certain level, the control component 106 controls the corresponding suction component 102 and blowing component 103 to achieve blowing and suction. Then, the liquid is repeatedly suctioned and blown by the set PLC control to achieve ATF perfusion. It will not cause shearing of cells or contaminate cells. It solves the problem that the existing ATF perfusion equipment uses peristaltic pumps and piston pumps to make repeated movements, which will result in a large equipment area and cumbersome operation.

[0024] The pressure reducing valve 112 is connected to the clean compressed air receiver 111 and is located on one side of the clean compressed air receiver 111; the vacuum generator 113 is disposed on one side of the pressure reducing valve 112, the clean compressed air receiver 111 is used to receive clean compressed air, and then the vacuum generator 113 vents air.

[0025] The vacuum generator 114 is connected to the pressure reducing valve 112 and is located on one side of the pressure reducing valve 112; the first filter 115 is connected to the vacuum generator 114 and is located on one side of the vacuum generator 114. When it is necessary to blow liquid through the air blowing component 103, clean compressed air is blown gently through the pressure reducing valve 112 (0.1 MPa). The first filter 115 is used to filter gas and keep the gas clean.

[0026] The one-way suction valve 116 is connected to the first filter 115 and is located on one side of the first filter 115; the suction pipe 117 is connected to the one-way suction valve 116 and the three-way solenoid valve 104, and is located on one side of the one-way suction valve 116. The one-way suction valve 116 is used to provide one-way suction of the suction pipe 117.

[0027] The one-way air valve 118 is connected to the first filter 115 and is located on one side of the first filter 115; the air pipe 119 is connected to the one-way air valve 118 and the three-way solenoid valve 104, and is located on one side of the one-way air valve 118. The one-way air valve 118 is used to provide one-way airflow to the air pipe 119.

[0028] The motion tube 120 is connected to the three-way solenoid valve 104 and is located on one side of the three-way solenoid valve 104; the sterilization filter 121 is connected to the motion tube 120 and is located on one side of the motion tube 120; the buffer tube 122 is connected to the sterilization filter 121 and is located on one side of the sterilization filter 121. The motion tube 120, in conjunction with the sterilization filter 121, is connected to the three-way solenoid valve 104. The buffer tube 122 is used to realize the blowing and suction of liquid and is made of 316L stainless steel (approximately 80cm long and 5cm in diameter).

[0029] The upper liquid level switch 123 is fixedly connected to the buffer tube 122 and is located on one side of the buffer tube 122; the lower liquid level switch 124 is fixedly connected to the buffer tube 122 and is located on the side of the buffer tube 122 away from the upper liquid level switch 123. When the liquid reaches the upper liquid level switch 123 at the upper end of the buffer tube 122, the air source of the vacuum generator 114 is sensed and disconnected, and the action is changed to blowing. Clean compressed air is gently blown by the pressure reducing valve 112 (0.1 MPa). When the liquid reaches the lower liquid level switch 124 at the bottom of the buffer tube 122, the clean compressed air is sensed and disconnected, and the action is changed to suction.

[0030] The first connecting pipe 125 is connected to the buffer pipe 122 and is located on one side of the buffer pipe 122; the first solenoid valve 126 is connected to the first connecting pipe 125 and the hollow fiber column 108 and is located on one side of the first connecting pipe 125. The first connecting pipe 125 cooperates with the first solenoid valve 126 to connect the buffer pipe 122 and the hollow fiber column 108.

[0031] The second connecting pipe 127 is connected to the buffer pipe 122 and is located on one side of the hollow fiber column 108; the second solenoid valve 128 is connected to the second connecting pipe 127 and the reactor 110, and is located on one side of the second connecting pipe 127. The second connecting pipe 127 cooperates with the second solenoid valve 128 to connect the hollow fiber column and the reactor 110.

[0032] When using this invention, the power structure 101 provides power to the suction component 102 or the blowing component 103. The vacuum generator 114 is pre-ventilated to draw the liquid in the reactor 110 into the hollow fiber column and then into the buffer tube 122. When the liquid reaches the upper liquid level switch 123 at the upper end of the buffer tube 122, the air source of the vacuum generator 114 is sensed and disconnected, and the action is changed to blowing. Clean compressed air is gently blown through the pressure reducing valve 112 (0.1MPa) to blow the liquid. When the liquid reaches the lower liquid level switch 124 at the bottom of the buffer tube 122, the clean compressed air is sensed and disconnected, and the action is changed to suction. Then, the liquid is repeatedly suctioned and blown by the set PLC control to achieve ATF perfusion without shearing or contaminating the cells. This solves the problem that existing ATF perfusion equipment uses peristaltic pumps and piston pumps for repeated movement, which results in a large equipment area and cumbersome operation.

[0033] The above-disclosed embodiments are merely one or more preferred embodiments of this application and should not be construed as limiting the scope of this application. Those skilled in the art can understand that all or part of the processes for implementing the above embodiments and equivalent changes made in accordance with the claims of this application still fall within the scope of this application.

Claims

1. A pneumatic ATF irrigation device, characterized in that, It includes a power structure, an intake component, an air blowing component, a three-way solenoid valve, a buffer component, a control component, a first connector, a hollow fiber column, a second connector, and a reactor; The suction component is disposed on one side of the power structure; the blowing component is disposed on one side of the power structure; the three-way solenoid valve is connected to the suction component and the blowing component respectively, and is located on one side of the suction component and the blowing component; the buffer component is disposed on one side of the three-way solenoid valve; the control component is disposed on one side of the buffer component; the first connecting component is disposed on one side of the buffer component; the hollow fiber column is connected to the first connecting component and is located on one side of the first connecting component; the second connecting component is disposed on one side of the hollow fiber column; the reactor is connected to the second connecting component and is located on one side of the second connecting component.

2. The pneumatic ATF irrigation device as described in claim 1, characterized in that, The power structure includes a clean compressed air receiver, a pressure reducing valve, and a vacuum generator. The pressure reducing valve is connected to the clean compressed air receiver and is located on one side of the clean compressed air receiver. The vacuum generator is located on one side of the pressure reducing valve.

3. The pneumatic ATF irrigation device as described in claim 2, characterized in that, The vacuum generating component includes a vacuum generator and a first filter. The vacuum generator is connected to the pressure reducing valve and is located on one side of the pressure reducing valve. The first filter is connected to the vacuum generator and is located on one side of the vacuum generator.

4. The pneumatic ATF irrigation device as described in claim 3, characterized in that, The suction component includes a suction one-way valve and a suction pipe. The suction one-way valve is connected to the first filter and is located on one side of the first filter. The suction pipe is connected to the suction one-way valve and also to the three-way solenoid valve, and is located on one side of the suction one-way valve.

5. The pneumatic ATF irrigation device as described in claim 4, characterized in that, The air blowing component includes an air blowing one-way valve and an air blowing pipe. The air blowing one-way valve is connected to the first filter and is located on one side of the first filter. The air blowing pipe is connected to the air blowing one-way valve and also to the three-way solenoid valve, and is located on one side of the air blowing one-way valve.

6. The pneumatic ATF irrigation device as described in claim 5, characterized in that, The buffer component includes a motion tube, a sterilization filter, and a buffer tube. The motion tube is connected to the three-way solenoid valve and is located on one side of the three-way solenoid valve. The sterilization filter is connected to the motion tube and is located on one side of the motion tube. The buffer tube is connected to the sterilization filter and is located on one side of the sterilization filter.

7. A pneumatic ATF irrigation device as described in claim 6, characterized in that, The control unit includes an upper liquid level switch and a lower liquid level switch. The upper liquid level switch is fixedly connected to the buffer tube and is located on one side of the buffer tube. The lower liquid level switch is fixedly connected to the buffer tube and is located on the side of the buffer tube away from the upper liquid level switch.

8. The pneumatic ATF irrigation device as described in claim 7, characterized in that, The first connector includes a first connecting pipe and a first solenoid valve. The first connecting pipe is connected to the buffer pipe and is located on one side of the buffer pipe. The first solenoid valve is connected to the first connecting pipe and to the hollow fiber column, and is located on one side of the first connecting pipe.

9. A pneumatic ATF irrigation device as described in claim 8, characterized in that, The second connector includes a second connecting pipe and a second solenoid valve. The second connecting pipe is connected to the buffer pipe and is located on one side of the hollow fiber column. The second solenoid valve is connected to the second connecting pipe and to the reactor, and is located on one side of the second connecting pipe.

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