A suspended cell bioreactor with temperature control function
Patent Information
- Application Number
- CN202522626051.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-11
- Publication Date
- 2026-10-30
- Estimated Expiration
- 2035-12-11
AI Technical Summary
[0003]其中，悬浮细胞(如CHO细胞、杂交瘤细胞)对培养环境温度极为敏感，其活性维持、增殖速率及代谢产物(如目标蛋白)生成效率，均依赖于37℃±0.5℃的恒定温度环境，现有反应器的控温方式多采用外部环绕式加热膜或底部单点加热，前者易因加热膜贴合度不均导致反应器内壁局部温差超过2℃，后者则会形成“底部高温、顶部低温”的温度梯度，造成细胞在反应器内分布不均，高温区域细胞易出现热应激反应，活性显著下降；低温区域细胞增殖周期延长，甚至停滞，最终导致整体细胞密度降低30％-50％，代谢产物产量波动幅度超过40％，严重影响实验重复性与工业化生产稳定性；
[0018]通过控温组件对加热溶液进行恒温控制，并将其输送至加热腔，为反应器内的悬浮细胞提供稳定、均匀的温度环境，避免温度波动对细胞活性、增殖效率及代谢产物生成造成不利影响，满足细胞培养对温度精准性的核心需求；灵活调节反应器位置，提升操作便利性：升降机构可推动反应器在加热腔与操作台顶部之间自由切换，需要控温培养时将反应器推入加热腔，利用恒温溶液维持温度；需取样、加料或清洁时将反应器推出加热腔，无需拆卸设备即可完成操作，简化流程并降低对细胞培养环境的干扰。
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Figure CN224917815U_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of suspended cell bioreactor technology, and particularly relates to a suspended cell bioreactor with temperature control function. Background Technology
[0002] Currently, in the fields of bioengineering and cell biology research, suspension cell culture is a core technology for preparing biopharmaceuticals (such as antibodies, vaccines, and recombinant proteins) and conducting cell function research. Suspension cell bioreactors, as key equipment for achieving large-scale, efficient culture, directly determine cell culture quality and experimental efficiency through their temperature control precision and ease of operation. However, current mainstream suspension cell bioreactors still have some problems in practical applications.
[0003] Suspension cells (such as CHO cells and hybridoma cells) are extremely sensitive to the temperature of the culture environment. Their activity maintenance, proliferation rate, and the efficiency of metabolite (such as target protein) production all depend on a constant temperature environment of 37℃±0.5℃. Existing reactors mostly use external surrounding heating films or bottom single-point heating for temperature control. The former is prone to local temperature differences exceeding 2℃ on the inner wall of the reactor due to uneven adhesion of the heating film, while the latter will form a temperature gradient of "high temperature at the bottom and low temperature at the top", resulting in uneven distribution of cells in the reactor. Cells in high-temperature areas are prone to heat stress response and significant decrease in activity; cell proliferation cycles are prolonged or even stopped in low-temperature areas, ultimately leading to a 30%-50% reduction in overall cell density and a fluctuation of more than 40% in metabolite production, which seriously affects experimental reproducibility and industrial production stability.
[0004] Although some reactors are equipped with basic temperature control components, they lack a heating solution circulation mechanism. After heating, the solution remains statically in the heating area, which cannot achieve uniform heat transfer. Furthermore, the temperature fluctuation range of the heating area can reach ±1.5℃ due to changes in ambient temperature (such as the start and stop of laboratory air conditioning and airflow disturbances), which further exacerbates the instability of the cell culture environment and makes it difficult to meet the culture requirements of highly sensitive cells (such as stem cells and primary cells).
[0005] To address these issues, we propose a suspended cell bioreactor with temperature control capabilities. Utility Model Content
[0006] The purpose of this invention is to address the aforementioned technical problems by providing a suspended cell bioreactor with temperature control function, thereby achieving the effect of providing a stable and uniform temperature environment.
[0007] In view of this, the present invention provides a suspended cell bioreactor with temperature control function, comprising: an operating platform, a heating chamber on the top of the operating platform, a reactor assembled inside the heating chamber, a temperature control component connected to one side of the heating chamber via a conduit, the temperature control component being used to maintain the temperature of the heating solution and to transport the heating solution into the heating chamber via the conduit to heat the reactor; a lifting chamber connected to the bottom of the heating chamber, a lifting mechanism installed inside the lifting chamber, the lifting mechanism being used to push the reactor up and down, not only pushing the reactor into the heating chamber with the constant temperature solution, but also pushing the reactor out of the heating chamber, distributed on the top of the operating platform.
[0008] Furthermore, the temperature control component includes a heating box installed on one side of the operating table, the heating box containing a heating solution, a heating cover fixedly installed on the outside of the heating box, a heating component installed inside the heating cover, a cover plate detachably installed on the top of the heating box, a second pump fixedly installed on one side of the top of the cover plate, a fifth pipe connected to the outlet end of the second pump, the other end of the fifth pipe connected to the top of the heating chamber, and a first water inlet pipe connected to the inlet end of the second pump, the first water inlet pipe penetrating the cover plate and distributed on the bottom side inside the heating box.
[0009] Furthermore, the heating assembly includes an electric heating wire fixedly installed on the inner wall of the heating cover, the electric heating wire being distributed in a spring-like manner on the inner wall of the heating cover.
[0010] Furthermore, a sixth pipe is connected and installed at the bottom of the heating chamber, and the other end of the sixth pipe passes through the cover plate and is connected and installed in the heating box.
[0011] Furthermore, mounting seats are rotatably mounted on both sides of the reactor via a rotating shaft. The mounting seats are concave in shape, and screws are threaded onto the side walls of the mounting seats. The other end of the screws abuts against the side walls of the reactor.
[0012] Furthermore, the lifting mechanism includes a sealing plug slidably installed inside the lifting cavity. Each of the four corners of the top of the sealing plug is fixedly connected to a support rod. The other ends of the four support rods pass through the middle of the operating table and are fixedly connected to the top of the mounting base. A first pipe is connected to one end of the top of the lifting cavity, and a second pipe is connected to one end of the bottom of the lifting cavity. The first pipe and the second pipe are respectively connected to the branch interface of a three-way connector. The three-way connector is connected to the outlet port of the first pump. A second water inlet pipe is connected to the inlet port of the first pump. The second water inlet pipe passes through the cover plate and is distributed on the bottom side inside the heating box.
[0013] Furthermore, a third pipe is connected to the other end of the top of the lifting cavity, and a fourth pipe is connected to the other end of the bottom of the lifting cavity. Both the third pipe and the fourth pipe penetrate the top of the side wall of the heating box and communicate with the inner cavity of the heating box.
[0014] Furthermore, four limiting holes are provided between the heating chamber and the lifting chamber, and a sealing ring is fixedly installed on the inner wall of the limiting hole, with the inner wall of the sealing ring fitting against the outer wall of the support rod.
[0015] Furthermore, a liquid level sensor is fixedly installed inside the heating chamber, and temperature sensors are fixedly installed on both the inner wall of the heating chamber and the inner wall of the heating box.
[0016] Furthermore, electric valves are installed on the first, second, third, fourth, fifth, and sixth pipes.
[0017] The beneficial effects of this utility model are:
[0018] The temperature control component maintains a constant temperature for the heating solution and delivers it to the heating chamber, providing a stable and uniform temperature environment for the suspended cells in the reactor. This avoids the adverse effects of temperature fluctuations on cell activity, proliferation efficiency, and metabolite generation, meeting the core requirement of temperature precision in cell culture. The reactor position can be flexibly adjusted to improve operational convenience: the lifting mechanism allows the reactor to freely switch between the heating chamber and the top of the operating table. When temperature-controlled culture is required, the reactor is pushed into the heating chamber, where the constant-temperature solution maintains the temperature. When sampling, feeding, or cleaning is needed, the reactor is pushed out of the heating chamber, allowing operations to be completed without disassembling the equipment, simplifying the process and reducing interference with the cell culture environment. Attached Figure Description
[0019] Figure 1 This is a schematic diagram of the overall structure of a suspended cell bioreactor with temperature control function proposed in this utility model;
[0020] Figure 2 This is a schematic diagram of the heating box structure of a suspended cell bioreactor with temperature control function proposed in this utility model;
[0021] Figure 3 This is a schematic diagram of the operating platform structure of a suspended cell bioreactor with temperature control function proposed in this utility model;
[0022] Figure 4 This is a schematic diagram of the mounting base structure for a suspended cell bioreactor with temperature control function proposed in this utility model;
[0023] The markings in the diagram are as follows:
[0024] 1. Operating platform; 11. Heating chamber; 12. Mounting base; 13. Reactor; 14. Support rod; 15. Sealing plug; 16. Lifting chamber; 17. Limiting hole; 18. Screw; 2. First pipe; 21. Second pipe; 22. T-connector; 23. Heating box; 24. Cover plate; 25. First pump; 26. Third pipe; 27. Fourth pipe; 3. Heating cover; 31. Second pump; 32. Fifth pipe; 33. Liquid level sensor; 34. Temperature sensor; 35. Sixth pipe. Detailed Implementation
[0025] The technical solutions of the embodiments of this application will be clearly described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this application. All other embodiments obtained by those skilled in the art based on the embodiments of this application are within the scope of protection of this application.
[0026] In the description of this application, it should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. For ease of description, the dimensions of the various parts shown in the drawings are not drawn to actual scale. Techniques, methods, and devices known to those skilled in the art may not be discussed in detail, but where appropriate, such techniques, methods, and devices should be considered part of the specification. In all examples shown and discussed herein, any specific values should be interpreted as merely exemplary and not as limitations. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters in the following drawings denote similar items; therefore, once an item is defined in one drawing, it need not be further discussed in subsequent drawings.
[0027] It should be noted that the terms "first," "second," etc., used in the specification and claims of this application are used to distinguish similar objects and not to describe a specific order or sequence. It should be understood that such use of data can be interchanged where appropriate so that embodiments of this application can be implemented in orders other than those illustrated or described herein, and the objects distinguished by "first," "second," etc., are generally of the same class and are not limited in number; for example, a first object can be one or more. Furthermore, in the specification and claims, "and / or" indicates at least one of the connected objects, and the character " / " generally indicates that the preceding and following objects are in an "or" relationship.
[0028] It should be noted that in the description of this application, the directional terms such as "front, back, up, down, left, right", "horizontal, vertical, horizontal" and "top, bottom" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description. Unless otherwise stated, these directional terms do not indicate or imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on the scope of protection of this application. The directional terms "inner" and "outer" refer to the inner and outer contours relative to the outline of each component itself.
[0029] It should be noted that, in this application, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes that element. Furthermore, it should be noted that the scope of the methods and apparatuses in the embodiments of this application is not limited to performing functions in the order shown or discussed, but may also include performing functions substantially simultaneously or in the reverse order, depending on the functions involved. For example, the described methods may be performed in a different order than described, and various steps may be added, omitted, or combined. Additionally, features described with reference to certain examples may be combined in other examples.
[0030] Reference Figures 1 to 4 A suspended cell bioreactor with temperature control function, comprising:
[0031] The operating table 1 has a heating chamber 11 on its top. A reactor 13 is installed inside the heating chamber 11. A temperature control component is installed on one side of the heating chamber 11 through a conduit. The temperature control component is used to control the heating solution at a constant temperature and to transport the heating solution into the heating chamber 11 through the conduit to heat the reactor 13.
[0032] The bottom of the heating chamber 11 is connected to a lifting chamber 16, and a lifting mechanism is installed inside the lifting chamber 16. The lifting mechanism is used to push the reactor 13 up and down, not only pushing the reactor 13 into the heating chamber 11 with a constant temperature solution, but also pushing the reactor 13 out of the heating chamber 11, which is distributed on the top of the operating table 1.
[0033] This application is approved. The temperature control component first regulates the temperature of the heating solution (e.g., heating, heat preservation) to maintain it at the constant temperature required for cell culture. Then, the constant temperature heating solution is transported to the heating chamber 11 at the top of the operating table 1 through a conduit. The heating chamber 11 surrounds the outer wall of the reactor 13 and transfers heat to the suspended cell culture system inside the reactor 13 through thermal conduction, stabilizing the system temperature within the target range and avoiding temperature fluctuations. The lifting principle of the reactor 13 is as follows: After the lifting mechanism inside the lifting chamber 16 is activated, the driving force acts on the bottom or support structure of the reactor 13. When temperature control is required, the driving force pushes the reactor 13 downward along the inner wall of the heating chamber 11 until it is completely immersed in the constant temperature solution inside the heating chamber 11. When it is necessary to operate the reactor 13, the driving force acts in the opposite direction, pushing the reactor 13 upward from the heating chamber 11 until it stops at a position at the top of the operating table 1 for easy operation.
[0034] In the example of this application, the temperature control component includes a heating box 23 installed on one side of the operating table 1. The heating box 23 contains a heating solution. A heating cover 3 is fixedly installed on the outside of the heating box 23. A heating component is installed inside the heating cover 3. A cover plate 24 is detachably installed on the top of the heating box 23. Multiple mounting holes are provided on the top of the heating box 23 and the cover plate 24. Bolts are threaded into the corresponding mounting holes. A second pump 31 is fixedly installed on one side of the top of the cover plate 24. A fifth pipe 32 is connected to the outlet end of the second pump 31. The other end of the fifth pipe 32 is connected to the top of the heating chamber 11. A first water inlet pipe is connected to the inlet end of the second pump 31. The first water inlet pipe passes through the cover plate 24 and is distributed on the bottom side inside the heating box 23.
[0035] As a preferred example of this utility model, the heating chamber 23 serves as an independent container for storing the heating solution. The heating cover 3 encloses the outer wall of the heating chamber 23 to form an insulation layer, reducing heat loss from the heating chamber 23 to the outside. The heating components inside the heating cover 3 generate heat when powered on, which is transferred to the solution inside the heating chamber 23 via heat conduction, gradually raising the solution temperature to the target value, thus achieving independent heating and insulation of the heating solution. The solution circulation principle is as follows: after the second pump 31 starts, it generates negative pressure, which, through the first water inlet pipe extending from the cover plate 24 to the bottom of the heating chamber 23, pumps the solution already heated to the target temperature into the heating chamber 23. The solution is drawn into the pump body; then the second pump 31 delivers the constant temperature solution to the top of the heating chamber 11 through the fifth pipe 32 at the outlet end. The solution flows downward from the top of the heating chamber 11, gradually filling the heating chamber 11 and enveloping the reactor 13, thus completing the heat transfer. When it is necessary to maintain the internal components of the heating box 23 (such as cleaning the inner wall, repairing the heating components) or replenish the heating solution, the staff can remove the detachable cover plate 24 on the top of the heating box 23 to directly access the internal space of the heating box 23. After maintenance or replenishment, the cover plate 24 is reinstalled to ensure the sealing of the heating box 23 and prevent heat loss and solution contamination.
[0036] In the example of this application, the heating assembly includes an electric heating wire fixedly installed on the inner wall of the heating cover 3, the electric heating wire being distributed in a spring-like manner on the inner wall of the heating cover 3.
[0037] As a preferred example of this utility model, the electric heating wire can be an iron-chromium-aluminum alloy electric heating wire. The electric heating wire is fixed to the inner wall of the heating cover 3 with a spring-shaped structure. Compared with the straight structure, the spring-shaped design greatly increases the contact area between the electric heating wire and the inner wall of the heating cover 3, and at the same time expands the indirect contact range with the solution in the heating chamber 23. The heat generated by the electric heating wire after being energized is quickly transferred to the solution in the heating chamber 23 through the inner wall of the heating cover 3. And because of the large contact area, the heat can be evenly distributed to various areas of the solution, avoiding local solution temperatures that are too high or too low.
[0038] In the example of this application, a sixth tube 35 is connected and installed at the bottom of the heating chamber 11, and the other end of the sixth tube 35 passes through the cover plate 24 and is connected and installed in the heating box 23.
[0039] As a preferred example of this utility model, the constant-temperature solution input at the top of the heating chamber 11, after completing heat exchange with the reactor 13, flows back to the heating box 23 naturally or by gravity through the sixth pipe 35 connected to the bottom of the heating chamber 11. The solution flowing back to the heating box 23 is heated to the target temperature again by the heating components, and then can be drawn by the second pump 31 and transported to the heating chamber 11 through the fifth pipe 32, forming a closed-loop circulation loop of heating box 23, first water inlet pipe, second pump 31, fifth pipe 32, heating chamber 11, sixth pipe 35, and heating box 23.
[0040] In the example of this application, mounting seats 12 are rotatably mounted on both sides of the reactor 13 via a rotating shaft. The mounting seats 12 are concave in shape, and a screw 18 is threaded onto the side wall of the mounting seat 12. The other end of the screw 18 abuts against the side wall of the reactor 13.
[0041] As a preferred example of this utility model, the concave mounting base 12 has its opening facing upwards, and the bottom or side wall of the reactor 13 can be embedded in the groove of the mounting base 12. The groove acts as a lateral limit for the reactor 13, preventing the reactor 13 from shifting left or right. Subsequently, the operator rotates the screw 18 on the side wall of the mounting base 12. The screw 18 moves along the threaded hole toward the reactor 13 until the end of the screw 18 is tightly attached to the side wall of the reactor 13 and generates a squeezing force. This squeezing force firmly fixes the reactor 13 in the groove of the mounting base 12, preventing the reactor 13 from shaking up and down or back and forth.
[0042] In the example of this application, the lifting mechanism includes a sealing plug 15 slidably installed in the lifting cavity 16. Each of the four corners of the top of the sealing plug 15 is fixedly connected to a support rod 14. The other ends of the four support rods 14 pass through the middle of the operating table 1 and are fixedly connected to the top of the mounting base 12. A first pipe 2 is connected to one end of the top of the lifting cavity 16, and a second pipe 21 is connected to one end of the bottom of the lifting cavity 16. The first pipe 2 and the second pipe 21 are respectively connected to the branch interface of the three-way connector 22. The three-way connector 22 is connected to the outlet port of the first pump 25. A second water inlet pipe is connected to the inlet port of the first pump 25. The second water inlet pipe passes through the cover plate 24 and is distributed inside the bottom side of the heating box 23.
[0043] As a preferred example of this utility model, after the first pump 25 is started, it draws solution from the heating tank 23 through the second water inlet pipe. The output direction of the first pump 25 is controlled according to the lifting requirements. If the reactor 13 needs to be lifted, the first pump 25 delivers the heating solution to the bottom of the lifting chamber 16 through the second pipe 21. The heating solution generates upward pressure at the bottom of the lifting chamber 16, pushing the sealing plug 15 to move upward along the inner wall of the lifting chamber 16. The sealing plug 15 drives the mounting base 12 and the reactor 13 to be lifted upward through the support rod 14. If the reactor 13 needs to be lowered, the first pump 25 delivers the heating solution to the top of the lifting chamber 16 through the first pipe 2. The heating solution generates downward pressure at the top of the lifting chamber 16, pushing the sealing plug 15 to move downward. The support rod 14 drives the reactor 13 to enter the heating chamber 11 downward.
[0044] In the example of this application, a third pipe 26 is connected to the other end of the top of the lifting cavity 16, and a fourth pipe 27 is connected to the other end of the bottom of the lifting cavity 16. Both the third pipe 26 and the fourth pipe 27 penetrate the top of the side wall of the heating box 23 and communicate with the inner cavity of the heating box 23.
[0045] As a preferred example of this utility model, when the sealing plug 15 moves upward in the lifting chamber 16, the volume of the space at the top of the lifting chamber 16 decreases and the pressure increases. Excess gas or solution is discharged into the heating chamber 23 through the third pipe 26 connecting the top of the lifting chamber 16 and the top of the heating chamber 23. Conversely, when the sealing plug 15 moves downward, the pressure at the bottom of the lifting chamber 16 increases and the solution is discharged into the heating chamber 23 through the fourth pipe 27.
[0046] In the example of this application, four limiting holes 17 are provided between the heating chamber 11 and the lifting chamber 16. A sealing ring is fixedly installed on the inner wall of the limiting hole 17, and the inner wall of the sealing ring is in contact with the outer wall of the support rod 14.
[0047] As a preferred example of this utility model, sealing rings are fixed to the inner walls of the four limiting holes 17 between the heating chamber 11 and the lifting chamber 16. The sealing rings are made of elastic material, and their inner walls are tightly fitted with the outer walls of the support rods 14 that penetrate the limiting holes 17 to form a sealing surface. When the support rods 14 move up and down along the limiting holes 17, the sealing rings always remain in contact with the outer walls of the support rods 14, preventing the solution in the heating chamber 11 or the lifting chamber 16 from leaking to the outside through the gap between the support rods 14 and the limiting holes 17. The four limiting holes 17 are symmetrically distributed between the heating chamber 11 and the lifting chamber 16. The support rods 14 penetrate the four limiting holes 17 respectively. The inner walls of the limiting holes 17 provide lateral restraint to the support rods 14, limiting the support rods 14 from shifting left, right, or forward and backward during the up and down movement. Guided by the limiting holes 17, the support rods 14 always move in the vertical direction, thereby driving the mounting base 12 and the reactor 13 to maintain a stable lifting trajectory and avoiding collision with the inner wall of the heating chamber 11.
[0048] In the example of this application, a liquid level sensor 33 is fixedly installed inside the heating chamber 11, and a temperature sensor 34 is fixedly installed on both the inner wall of the heating chamber 11 and the inner wall of the heating box 23.
[0049] As a preferred example of this utility model, the liquid level sensor 33 inside the heating chamber 11 collects the liquid level data of the solution inside the heating chamber 11 in real time to monitor the heating solution inside the heating chamber 11; the temperature sensors 34 on the inner walls of the heating chamber 11 and the heating box 23 respectively collect the real-time temperature data of the solution inside the chamber and feed the data back to the control processor; the control processor compares the real-time temperature with the preset target temperature. If the real-time temperature is lower than the target temperature, it controls the heating component to increase the heating power to accelerate the heating rate of the solution; if the real-time temperature is higher than the target temperature, it controls the heating component to reduce the heating power or stop heating to keep the solution temperature stably maintained within the target range, thereby achieving closed-loop temperature control.
[0050] In the example of this application, electric valves are installed on the first pipe 2, the second pipe 21, the third pipe 26, the fourth pipe 27, the fifth pipe 32, and the sixth pipe 35.
[0051] As a preferred example of this utility model, the electric valves on each pipe, such as the first pipe 2 and the second pipe 21, are electrically connected to the equipment control system. The operator can send switch or opening adjustment commands to individual electric valves through the control system. Through the linkage of multiple electric valves, the automated operation of processes such as solution circulation and reactor 13 lifting can be realized.
[0052] The embodiments of this application have been described above with reference to the accompanying drawings. Unless otherwise specified, the embodiments and features in the embodiments of this application can be combined with each other. This application is not limited to the specific embodiments described above. The specific embodiments described above are merely illustrative and not restrictive. Those skilled in the art can make many other forms under the guidance of this application without departing from the spirit and scope of the claims, and all of these forms are within the protection scope of this application.
Claims
1. A suspended cell bioreactor with temperature control function, characterized in that... ,include: An operating table (1) is provided with a heating chamber (11) on the top of the operating table (1). A reactor (13) is installed inside the heating chamber (11). A temperature control component is installed on one side of the heating chamber (11) through a conduit. The temperature control component is used to control the temperature of the heating solution and to transport the heating solution into the heating chamber (11) through the conduit to heat the reactor (13). The bottom of the heating chamber (11) is connected to a lifting chamber (16), and a lifting mechanism is installed inside the lifting chamber (16). The lifting mechanism is used to push the reactor (13) up and down, and can push the reactor (13) into the heating chamber (11) or push the reactor (13) to the top of the operating table (1).
2. A suspended cell bioreactor with temperature control function according to claim 1, characterized in that, The temperature control assembly includes a heating box (23) installed on one side of the operating table (1). The heating box (23) contains a heating solution. A heating cover (3) is fixedly installed on the outside of the heating box (23). A heating assembly is installed inside the heating cover (3). A cover plate (24) is detachably installed on the top of the heating box (23). A second pump (31) is fixedly installed on one side of the top of the cover plate (24). A fifth pipe (32) is connected to the outlet end of the second pump (31). The other end of the fifth pipe (32) is connected to the top of the heating chamber (11). A first water inlet pipe is connected to the inlet port of the second pump (31). The first water inlet pipe passes through the cover plate (24) and is distributed on the bottom side inside the heating box (23).
3. A suspended cell bioreactor with temperature control function according to claim 2, characterized in that, The heating assembly includes an electric heating wire fixedly installed on the inner wall of the heating cover (3), and the electric heating wire is distributed in a spring shape on the inner wall of the heating cover (3).
4. A suspended cell bioreactor with temperature control function according to claim 3, characterized in that, The bottom end of the heating chamber (11) is connected to a sixth pipe (35), and the other end of the sixth pipe (35) passes through the cover plate (24) and is connected to the heating box (23).
5. A suspended cell bioreactor with temperature control function according to claim 4, characterized in that, The reactor (13) has mounting seats (12) rotatably mounted on both sides via a rotating shaft. The mounting seats (12) are concave in shape. A screw (18) is threaded onto the side wall of the mounting seat (12). The other end of the screw (18) abuts against the side wall of the reactor (13).
6. A suspended cell bioreactor with temperature control function according to claim 5, characterized in that, The lifting mechanism includes a sealing plug (15) that is slidably installed in the lifting cavity (16). Each of the four corners of the top of the sealing plug (15) is fixedly connected to a support rod (14). The other ends of the four support rods (14) pass through the middle of the operating table (1) and are fixedly connected to the top of the mounting base (12). A first pipe (2) is connected to one end of the top of the lifting cavity (16), and a second pipe (21) is connected to one end of the bottom of the lifting cavity (16). The first pipe (2) and the second pipe (21) are respectively connected to the branch interface of the three-way connector (22). The three-way connector (22) is connected to the outlet port of the first pump (25). A second water inlet pipe is connected to the inlet port of the first pump (25). The second water inlet pipe passes through the cover plate (24) and is distributed on the bottom side inside the heating box (23).
7. A suspended cell bioreactor with temperature control function according to claim 6, characterized in that, The top end of the lifting chamber (16) is connected to a third pipe (26), and the bottom end of the lifting chamber (16) is connected to a fourth pipe (27). Both the third pipe (26) and the fourth pipe (27) penetrate the top of the side wall of the heating box (23) and communicate with the inner cavity of the heating box (23).
8. A suspended cell bioreactor with temperature control function according to claim 7, characterized in that, The heating chamber (11) and the lifting chamber (16) are connected by four limiting holes (17). A sealing ring is fixedly installed on the inner wall of the limiting hole (17), and the inner wall of the sealing ring is in contact with the outer wall of the support rod (14).
9. A suspended cell bioreactor with temperature control function according to claim 8, characterized in that, A liquid level sensor (33) is fixedly installed inside the heating chamber (11), and a temperature sensor (34) is fixedly installed on both the inner wall of the heating chamber (11) and the inner wall of the heating box (23).
10. A suspended cell bioreactor with temperature control function according to claim 9, characterized in that, Electric valves are installed on the first pipe (2), the second pipe (21), the third pipe (26), the fourth pipe (27), the fifth pipe (32), and the sixth pipe (35).