Bubble-proof constant-speed controllable-temperature animal perfusion peristaltic pump

Abstract

The utility model relates to peristaltic pump technical field discloses a kind of anti-bubble constant-speed controllable temperature animal perfusion peristaltic pump, including peristaltic pump host computer, the peristaltic pump host computer includes drive arrangement and by the pump head driven of drive arrangement, the import end and export end of the pump head are respectively through hose detachably connected pulse controller, the pulse controller connected in the export end of the pump head detachably connected online air bubble device, the online air bubble device is used to eliminate the bubble in the liquid output by the pump head, the online air bubble device includes import connection pipe and export connection pipe, the import connection pipe is connected with the pulse controller intercommunication, the export connection pipe is connected telescopic constant-temperature heating device. The equipment of the utility model can be realized compact integration with existing perfusion system, realize continuous automatic air exhaust, meet the demand of high-precision hepatocyte perfusion experiment.

Description

Technical Field

[0001] This utility model relates to the field of peristaltic pump technology, and in particular to an anti-bubble type constant speed controllable temperature animal peristaltic pump. Background Technology

[0002] The existing constant-speed, temperature-controlled animal peristaltic pump (CN202420718248.0) effectively solves the problems of flow rate pulsation and temperature loss during the peristalsis process by setting pulse suppressors at the inlet and outlet ends of the pump head and a retractable constant-temperature heating device at the outlet end, which significantly reduces animal stress and improves experimental accuracy.

[0003] However, existing devices still have shortcomings in actual in situ hepatocyte perfusion and collagenase digestion processes: the peristaltic pump relies on rollers to periodically squeeze the tubing to deliver liquid, and this physical process inevitably introduces air into the liquid, forming tiny bubbles; at the same time, microleakage at the tubing connections also introduces bubbles. These bubbles, after entering the portal vein with the perfusion fluid, form local air emboli, blocking hepatic sinusoidal blood flow, leading to uneven distribution of collagenase, with some areas being over-digested while others are under-digested, ultimately causing massive hepatocyte death, decreased viability, and even failure of the entire primary cell preparation. Although existing technologies have independent degassing devices or manual exhaust valves, they cannot be compactly integrated with existing perfusion systems, and it is difficult to achieve continuous automatic venting while maintaining a constant speed, thus failing to meet the requirements of high-precision hepatocyte perfusion experiments. Utility Model Content

[0004] In view of the above-mentioned shortcomings in the current peristaltic pump technology, this utility model provides an anti-bubble type constant speed controllable temperature animal peristaltic pump that can be compactly integrated with existing peristalsis systems, realize continuous automatic venting, and meet the needs of high-precision hepatocyte peristalsis experiments.

[0005] To achieve the above objectives, the embodiments of this utility model adopt the following technical solutions:

[0006] An anti-bubble type constant speed controllable temperature animal peristaltic pump includes a peristaltic pump main unit. The peristaltic pump main unit includes a drive device and a pump head driven by the drive device. The inlet and outlet ends of the pump head are detachably connected to a pulse controller via flexible hoses. The pulse controller connected to the outlet end of the pump head is detachably connected to an online de-bubble device. The online de-bubble device is used to eliminate bubbles in the liquid output by the pump head. The online de-bubble device includes an inlet pipe and an outlet pipe. The inlet pipe is connected to the pulse controller, and the outlet pipe is connected to a retractable constant temperature heating device.

[0007] According to one aspect of the present invention, the online degassing device further includes a sealing cap, an expansion chamber, and a flow control valve; the sealing cap is detachably fitted onto the top of the expansion chamber, and the flow control valve is disposed on the outlet pipe for controlling the flow rate of the discharged liquid.

[0008] According to one aspect of the present invention, the expansion cavity is a vertically arranged transparent container.

[0009] According to one aspect of this utility model, the expansion cavity is a component made of a transparent polymer material.

[0010] According to one aspect of the present invention, the inlet pipe is disposed on the upper part of the side wall of the expansion cavity and extends into the interior of the expansion cavity, with its outlet facing the inner wall of the expansion cavity; the outlet pipe is disposed at the bottom center of the expansion cavity and is connected to the retractable constant temperature heating device through a pipeline.

[0011] According to one aspect of the present invention, the bottom of the expansion cavity is provided with an anti-vortex flow guiding structure or a microporous filter.

[0012] According to one aspect of the present invention, the sealing cover is provided with a venting structure, which is a pressure relief micropore or a detachable one-way exhaust valve.

[0013] According to one aspect of the present invention, the peristaltic pump main unit further includes a control device.

[0014] According to one aspect of the present invention, the control device includes a flow rate control panel and a temperature control panel.

[0015] According to one aspect of this utility model, the driving device is an electric motor.

[0016] Advantages of this invention: The bubble-containing liquid pumped out by the peristaltic pump enters the expansion chamber of the online bubble removal device after passing through the pulse controller. Since the cross-sectional area of ​​the chamber is much larger than that of the hose, the liquid flow rate drops sharply, and the bubbles rise rapidly under the action of buoyancy and gather at the top of the chamber. The valve opening is adjusted to make the outflow rate consistent with the set flow rate of the peristaltic pump. The bubble-free liquid is heated to the set temperature by the retractable constant temperature heating device and then injected into the animal body or organs at a constant rate. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a schematic diagram of the structure of an anti-bubble type constant speed controllable temperature animal peristaltic pump according to the present invention;

[0019] Figure 2 This is a schematic diagram of the online air bubble removal device for an anti-bubble type constant speed controllable temperature animal peristaltic pump according to the present invention.

[0020] Figure label:

[0021] 1. Peristaltic pump main unit; 2. Flow rate control panel; 3. Temperature control panel; 4. Pulse controller; 5. Pump head; 6. Online air bubble removal device; 61. Inlet pipe; 62. Sealing cap; 63. Expansion chamber; 64. Flow control valve; 65. Outlet pipe; 7. Retractable constant temperature heating device. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0023] like Figure 1 and Figure 2 As shown, an anti-bubble type constant-speed controllable temperature animal peristaltic pump includes a peristaltic pump main unit 1. The peristaltic pump main unit 1 includes a drive device and a pump head 5 driven by the drive device. The inlet and outlet ends of the pump head 5 are detachably connected to a pulse controller 4 via flexible hoses. The pulse controller 4, connected to the outlet end of the pump head 5, is detachably connected to an online de-bubble device 6. The online de-bubble device 6 is used to eliminate bubbles in the liquid output by the pump head 5. The online de-bubble device 6 includes an inlet pipe 61 and an outlet pipe 65. The inlet pipe 61 is connected to the pulse controller 4, and the outlet pipe 65 is connected to a retractable constant-temperature heating device 7. By setting the online de-bubble device 6 downstream of the pulse controller 4, the bubble-containing liquid pumped by the peristaltic pump main unit 1 enters the online de-bubble device 6 after passing through the pulse controller 4 to remove bubbles, thereby eliminating the influence of bubbles in the peristaltic solution on the peristaltic effect. The retractable constant-temperature heating device 7 heats the peristaltic solution to a set temperature before infusing it into the animal or organ, ensuring that the peristaltic temperature is at the same level as the animal's body temperature or organ temperature, reducing the animal's stress response caused by bubbles and temperature fluctuations.

[0024] In practical use, the online deaerator 6 also includes a sealing cap 62, an expansion chamber 63, and a flow control valve 64. The sealing cap 62 is detachably fitted onto the top of the expansion chamber 63, and the flow control valve 64 is located on the outlet pipe 65 to control the flow rate of the discharged liquid. In use, the sealing cap 62 forms a sealed chamber after being fitted onto the top of the expansion chamber 63. The aerated liquid pumped by the peristaltic pump 1 enters the expansion chamber 63 of the online deaerator 6 after passing through the pulse controller 4. Since the cross-sectional area of ​​the chamber is much larger than that of the hose, the liquid flow rate drops sharply, and the bubbles rise rapidly under the action of buoyancy and gather at the top of the chamber. After the liquid level rises to a certain height, the flow control valve 64 on the outlet pipe 65 is opened, and the valve opening is adjusted to make the outflow rate consistent with the set flow rate of the peristaltic pump.

[0025] In practical use, the expansion chamber 63 is a vertically arranged transparent container. During use, the volume of the expansion chamber 63 is 10 to 50 times the volume of a single pulse from the flexible tube.

[0026] In practical use, the expansion chamber 63 is a component made of transparent polymer material, which can be made of polycarbonate or acrylic, making it easy to observe the liquid level and bubble accumulation inside the expansion chamber 63.

[0027] In practical use, the inlet pipe 61 is located on the upper part of the side wall of the ventilation structure and extends into the expansion cavity 63, with its outlet facing the inner wall of the expansion cavity 63; the outlet pipe 65 is located at the bottom center of the expansion cavity 63 and is connected to the retractable constant temperature heating device 7 via a pipeline. The inlet pipe 61 extending into the inner wall of the expansion cavity 63 is designed to reduce liquid flow impact disturbance and facilitate bubble release.

[0028] In practical use, the bottom of the expansion chamber 63 is equipped with an anti-vortex flow guiding structure or a microporous filter. During use, the anti-vortex flow guiding structure or microporous filter can prevent rising air bubbles from being sucked into the outlet pipe 65.

[0029] In practical use, the sealing cap 62 is equipped with a venting structure, which can be a pressure relief micro-hole or a detachable one-way exhaust valve. The venting structure is used to expel air from the expansion chamber 63 during the initial injection phase and to maintain a tight seal after venting is completed.

[0030] In practical use, the peristaltic pump main unit 1 also includes a control device.

[0031] In practical use, the control device includes a flow rate control panel 2 and a temperature control panel 3. The flow rate control panel 2 is used to control the flow rate of the peristaltic pump host 1 and can set the flow rate of the peristaltic pump host 1; the temperature control panel 3 is used to control the temperature of the peristaltic pump host 1 and can set the temperature of the peristaltic pump host 1.

[0032] In practical use, the driving device is an electric motor.

[0033] Furthermore, taking the isolation of primary rat hepatocytes as an example, the method of using this invention is described as follows:

[0034] Step S1: Clean and disinfect the peristaltic pump hose, pulse controller 4 and online de-bubbling device 6 using conventional methods (remove the sealing cap 62), assemble the pipeline, and connect the outlet pipe 65 of the online de-bubbling device 6 to the inlet of the retractable constant temperature heating device 7 through the pipeline;

[0035] Step S2: Turn on the peristaltic pump 1 and pump the pre-cooled perfusion fluid (such as D-Hanks fluid) at a low flow rate (e.g., 5 mL / min). The liquid enters the expansion chamber 63 through the inlet pipe 61, and the air is discharged from the sealing cap 62. After the liquid fills about 2 / 3 of the chamber, tighten the sealing cap 62.

[0036] Step S3: Slowly adjust the flow control valve 64 on the outlet pipe 65 to make the outflow rate consistent with the peristaltic pump set flow rate; observe that the bubbles in the expansion chamber 63 rise and gather at the top, and the liquid level remains stable;

[0037] Step S4: Set the temperature of the retractable constant temperature heating device 7 to 39°C. After the temperature stabilizes, connect the pumping hose to the rat portal vein indwelling needle and begin in situ perfusion.

[0038] Step S5: When switching to collagenase digestion solution during the perfusion stage, there is no need to interrupt the process. Air bubbles continue to be trapped in the expansion chamber 63 to ensure that the digestion solution entering the liver is free from air bubble interference.

[0039] Step S6: After the perfusion is completed, turn off the peristaltic pump, disconnect the pipeline connection, disassemble the online air bubble removal device 6, clean and disinfect it, and set it aside for later use.

[0040] Advantages of this invention: The bubble-containing liquid pumped out by the peristaltic pump 1 enters the expansion chamber 63 of the online bubble removal device 6 after passing through the pulse controller 4. Since the cross-sectional area of ​​the chamber is much larger than the cross-sectional area of ​​the hose, the liquid flow rate drops sharply, and the bubbles rise rapidly under the action of buoyancy and gather at the top of the chamber. The opening of the flow control valve 64 is adjusted to make the outflow rate consistent with the set flow rate of the peristaltic pump. The bubble-free liquid is heated to the set temperature by the retractable constant temperature heating device 7 and then injected into the animal body or organs at a constant rate.

[0041] The above description is merely a specific embodiment of this utility model, but the protection scope of this utility model is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this utility model should be included within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the protection scope of the claims.

Claims

1. A bubble-proof, constant-speed, temperature-controlled animal peristaltic pump, comprising a peristaltic pump main unit, the peristaltic pump main unit including a drive device and a pump head driven by the drive device, wherein the inlet end and outlet end of the pump head are detachably connected to a pulse controller via flexible hoses, characterized in that, The pulse controller connected to the outlet end of the pump head is detachably connected to an online de-aeration device. The online de-aeration device is used to eliminate air bubbles in the liquid output by the pump head. The online de-aeration device includes an inlet pipe and an outlet pipe. The inlet pipe is connected to the pulse controller, and the outlet pipe is connected to a retractable constant temperature heating device. The online de-aeration device also includes a sealing cap, an expansion chamber, and a flow control valve. The sealing cap is detachably fitted onto the top of the expansion chamber, and the flow control valve is located on the outlet pipe to control the flow rate of the discharged liquid.

2. The anti-bubble type constant speed controllable temperature animal peristaltic pump according to claim 1, characterized in that, The expansion cavity is a vertically arranged transparent container.

3. The anti-bubble type constant speed controllable temperature animal peristaltic pump according to claim 2, characterized in that, The inlet pipe is located on the upper part of the side wall of the expansion cavity and extends into the interior of the expansion cavity, with its outlet facing the inner wall of the expansion cavity; the outlet pipe is located at the bottom center of the expansion cavity and is connected to the retractable constant temperature heating device through a pipeline.

4. The anti-bubble type constant speed controllable temperature animal peristaltic pump according to claim 3, characterized in that, The bottom of the expansion cavity is equipped with an anti-vortex flow guiding structure or a microporous filter.

5. The anti-bubble type constant speed controllable temperature animal peristaltic pump according to claim 4, characterized in that, The sealing cover is provided with a venting structure, which is a pressure relief micro-hole or a detachable one-way exhaust valve.

6. The anti-bubble type constant speed controllable temperature animal peristaltic pump according to claim 1, characterized in that, The peristaltic pump unit also includes a control device.

7. The anti-bubble type constant speed controllable temperature animal peristaltic pump according to claim 6, characterized in that, The control device includes a flow rate control panel and a temperature control panel.

8. The anti-bubble type constant speed controllable temperature animal peristaltic pump according to claim 1, characterized in that, The driving device is an electric motor.

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