A disposable cell counting plate
Patent Information
- Application Number
- CN202522462512.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-20
- Publication Date
- 2026-10-30
- Estimated Expiration
- 2035-11-20
AI Technical Summary
[0003]现有一次性细胞计数板缺乏明确的定位标识，仅依赖计数室自身的网格结构进行寻找，显微镜观测时，需先通过低倍镜反复移动载物台、调焦，才能找到计数室位置，无法快速对准计数室中心区域，切换高倍镜后仍需二次微调，操作繁琐，且细胞计数板加样时，细胞悬液需通过毛细作用铺满计数室，多余液体会残留于板体表面或缝隙，现有计数板无专用自带的吸附结构，需额外取用无尘纸、棉签等工具擦拭，降低操作效率
1、本装置在转移时，使用食指和拇指与U型定位槽贴合，拿起本装置，U型定位槽的形状与手指能够更好的贴合，提升接触面积并更方便施力，同时防滑纹的设计提升了U型定位槽与操作者手指之间的摩擦力，能够更稳定和方便的将本装置从桌面上拿起并移动，将本装置放置在显微镜载物台上后，还可将定位夹具插入U型定位槽内侧，通过U型定位槽对本装置进行更稳固的夹持，防止打滑。
Smart Images
Figure CN224921641U_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of cell counting plate technology, specifically relating to a disposable cell counting plate. Background Technology
[0002] Cell counting chambers are core tools for quantitative cell analysis in fields such as biology, medicine, and immunology. They are widely used in cell culture, drug development, and clinical testing. Their core requirement is to achieve "fast, accurate, and convenient" cell counting, ensuring the accuracy and reproducibility of experimental data.
[0003] Existing disposable cell counting chambers lack clear positioning markers and rely solely on the grid structure of the counting chamber itself for location. During microscope observation, it is necessary to repeatedly move the stage and adjust the focus using a low-power microscope to locate the counting chamber, making it impossible to quickly align with the center area of the counting chamber. Even after switching to a high-power microscope, a second fine-tuning is still required, making the operation cumbersome. Furthermore, when adding samples to the cell counting chamber, the cell suspension needs to be spread throughout the counting chamber through capillary action, leaving excess liquid residue on the surface or in the gaps of the chamber. Existing counting chambers do not have a dedicated built-in adsorption structure, requiring the use of lint-free paper, cotton swabs, and other tools for wiping, which reduces operational efficiency. Utility Model Content
[0004] To address the above problems, the purpose of this invention is to provide a disposable cell counting chamber. When using a microscope to observe the counting chamber, this device can more quickly locate the center position of the counting chamber by setting cross-shaped positioning marks on the same straight line as the central axis of the counting chamber, thereby improving observation efficiency. The design with built-in dust-free paper eliminates the need to separately take out the dust-free paper, thus improving operational efficiency.
[0005] To achieve the above objectives, this utility model provides the following technical solution: a disposable cell counting plate, comprising a plate body, the top of which is provided with four symmetrical overflow grooves, a two-sided support platform is formed between the two overflow grooves on the left and the two overflow grooves on the right, and a central support platform is formed between the two overflow grooves in the middle, the central support platform having a dividing groove at the middle position downwards, the two ends of the dividing groove communicating with the overflow grooves on both sides of the central support platform, counting chambers being provided on both sides of the central support platform, the top of the central support platform having four sets of cover glass positioning seats, the four sets of cover glass positioning seats being symmetrically distributed left, right, front and back, and a cover glass being placed between the four sets of cover glass positioning seats, the top of the cover glass being covered with a protective film, and a dust-free paper being adhered to the top of the protective film, the two ends of the plate body having U-shaped positioning grooves, the inner side of the U-shaped positioning grooves having anti-slip textures, and the top of the two-sided support platforms having cross-shaped positioning marking lines.
[0006] The beneficial effects of this invention are as follows: When transferring the device, the index finger and thumb are used to contact the U-shaped positioning groove. The U-shaped groove fits the fingers better, increasing the contact area and facilitating force application. Simultaneously, the anti-slip texture design increases friction between the U-shaped groove and the operator's fingers, allowing for more stable and convenient lifting and movement of the device from the table. After placing the device on the microscope stage, a positioning clamp can be inserted into the U-shaped positioning groove for a more secure grip, preventing slippage. When observing the counting chamber, a low-power microscope can be used to quickly locate the crosshair positioning mark. The crosshair positioning mark, located on the same straight line as the central axis of the counting chamber, allows for faster centering of the chamber. Following the crosshair, the stage is finely adjusted horizontally to precisely align with the center area of the corresponding counting chamber. Switching to a high-power microscope allows direct observation of the grid and cells without repeatedly moving the stage or focusing to find the counting area, improving observation efficiency. This is particularly suitable for novice operators and high-throughput counting scenarios.
[0007] To improve the efficiency of observation in the counting chamber: As a further improvement to the above technical solution: the cross-shaped positioning mark lines are set above the support platforms on both sides, with a total of four sets, two sets corresponding to each counting chamber. The two sets of cross-shaped positioning mark lines corresponding to a single counting chamber are located on the same straight line of a central axis of the counting chamber and are equidistant from the center of the counting chamber. The cross-shaped positioning mark lines are recessed with a groove width of 0.5 mm.
[0008] The beneficial effects of this improvement are as follows: When observing the counting chamber, the position of the crosshair positioning mark can be quickly found using a low-power microscope. By setting the crosshair positioning mark on the same straight line as the central axis of the counting chamber, the center position of the counting chamber can be located more quickly. Under the guidance of the crosshair positioning mark, the stage can be finely adjusted in the horizontal direction to accurately align with the center area of the corresponding counting chamber. Then, by switching to a high-power microscope, the grid and cells can be directly observed without repeatedly moving the stage or focusing to find the counting area, thus improving observation efficiency. This is especially suitable for novice operation and high-throughput counting scenarios.
[0009] To avoid the need to separately take out the lint-free paper: As a further improvement to the above technical solution: the protective film is provided with easy-tear lines, which are distributed along the edge of the dust-free paper.
[0010] The beneficial effects of this improvement are as follows: After removing the protective film, tear off the part of the protective film outside the edge of the lint-free paper along the tear line, place the lint-free paper face down, and gently insert it into the overflow tank. This will absorb the excess liquid in the overflow tank and the dividing groove, eliminating the need to take out the lint-free paper separately and improving operational efficiency.
[0011] To make it easier to peel off the protective film: As a further improvement to the above technical solution: one end of the protective film is connected to a peeler.
[0012] The beneficial effect of this improvement is that the peeler makes it easier to peel off the protective film.
[0013] To secure the coverslip: As a further improvement to the above technical solution: the cover glass positioning seat is provided with a buckle receiving groove on the top, and the buckle receiving groove is provided with a buckle integrally formed with the cover glass positioning seat, and the buckle fastens the top edge of the cover glass.
[0014] The beneficial effects of this improvement are as follows: the coverslip positioning seat is used to position the four corners of the coverslip, so that the coverslip is stably clamped between the four coverslip positioning seats. The buckle is used to fix the coverslip. The buckle is elastic. By prying the buckle, it can be released from fixing the coverslip and the coverslip can be removed.
[0015] To prevent excess liquid from overflowing onto the outside of the plate: As a further improvement to the above technical solution: an overflow prevention barrier is provided above the edge of the plate.
[0016] The beneficial effect of this improvement is that during the sample addition process, excess liquid flows into the overflow tank and the dividing groove, and will not overflow to the outside of the plate under the obstruction of the overflow prevention barrier.
[0017] In order for the cell suspension to enter the counting chamber: As a further improvement to the above technical solution: the top surface height of the central support platform is 0.1 mm lower than the top surface height of the two side support platforms.
[0018] The top surface of the central support is 0.1 mm lower than the top surface of the two side support surfaces, so that the bottom of the coverslip forms a certain gap with the central support, allowing the cell suspension to enter the counting chamber.
[0019] In summary, the beneficial effects of this case are as follows: 1. When transferring this device, use your index finger and thumb to contact the U-shaped positioning groove. The shape of the U-shaped positioning groove fits the finger better, increasing the contact area and making it easier to apply force. At the same time, the anti-slip texture design increases the friction between the U-shaped positioning groove and the operator's fingers, making it easier and more stable to pick up and move the device from the table. After placing the device on the microscope stage, the positioning clamp can be inserted into the inside of the U-shaped positioning groove to clamp the device more firmly and prevent slippage.
[0020] 2. When using this device to observe the counting chamber under a microscope, you can first use a low-power microscope to quickly locate the position of the crosshair positioning marks. By using the crosshair positioning marks set on the same straight line as the central axis of the counting chamber, you can more quickly locate the center position of the counting chamber. Under the guidance of the crosshair positioning marks, you can finely adjust the stage in the horizontal direction to accurately align it with the center area of the corresponding counting chamber. Then, switch to a high-power microscope to directly observe the grid and cells without repeatedly moving the stage or focusing to find the counting area, thus improving observation efficiency. It is especially suitable for novice operation and high-throughput counting scenarios.
[0021] 3. After removing the protective film, tear off the part of the protective film outside the edge of the lint-free paper along the tear line. Place the lint-free paper face down and gently insert it into the overflow tank. This will absorb the excess liquid in the overflow tank and the dividing groove, eliminating the need to take out the lint-free paper separately and improving operational efficiency.
[0022] The parts of the device not covered herein are the same as or can be implemented using existing technologies. Attached Figure Description
[0023] Figure 1 This is a schematic diagram of the isometric structure of this utility model; Figure 2 This is a schematic diagram of the isometric separation structure of this utility model; Figure 3 This is a top view of the plate body in this utility model; Figure 4 This is a schematic diagram of the horizontal view structure of this utility model; Figure 5 This is a schematic diagram of the cover glass positioning seat and cover glass in this utility model; In the diagram: 1. Plate body; 2. Overflow trough; 3. Dividing groove; 4. Central support platform; 5. Side support platforms; 6. Counting chamber; 7. Coverslip positioning seat; 8. Clip; 9. Coverslip; 10. Protective film; 11. Dust-free paper; 12. Lifting plate; 13. Overflow prevention barrier; 14. U-shaped positioning groove; 15. Anti-slip texture; 16. Cross-shaped positioning mark line; 17. Easy-tear line. Detailed Implementation
[0024] To enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be described in detail below with reference to the accompanying drawings. The description in this part is only exemplary and explanatory, and should not be used to limit the scope of protection of the present invention in any way.
[0025] like Figure 1-5As shown, a disposable cell counting plate includes a plate body 1. The top of the plate body 1 has four symmetrical overflow grooves 2. A two-sided support platform 5 is formed between the two overflow grooves 2 on the left and between the two overflow grooves 2 on the right. A central support platform 4 is formed between the two overflow grooves 2 in the middle. A dividing groove 3 is provided downwards in the middle of the central support platform 4. The two ends of the dividing groove 3 are respectively connected to the overflow grooves 2 on both sides of the central support platform 4. The central support platform 4 is located between the dividing grooves 3. The sides are equipped with counting chambers 6. The top of the central support platform 4 is equipped with four sets of cover glass positioning seats 7, which are symmetrically distributed in the left, right and front and back. Cover glass 9 is placed between the four sets of cover glass positioning seats 7. The top of the cover glass 9 is covered with a protective film 10, and the top of the protective film 10 is attached with a dust-free paper 11. Both ends of the plate body 1 are equipped with U-shaped positioning grooves 14. The inner side of the U-shaped positioning grooves 14 is provided with anti-slip textures 15. The top of the two side support platforms 5 is provided with cross-shaped positioning mark lines 16.
[0026] When transferring this device, use your index finger and thumb to contact the U-shaped positioning groove 14. The shape of the U-shaped positioning groove 14 better fits your fingers, increasing the contact area and making it easier to apply force. Simultaneously, the anti-slip texture 15 increases the friction between the U-shaped positioning groove 14 and the operator's fingers, allowing for more stable and convenient lifting and movement of the device from the table. After placing the device on the microscope stage, a positioning clamp can be inserted into the inside of the U-shaped positioning groove 14 for a more secure grip, preventing slippage. When measuring counting chamber 6, you can first use a low-power microscope to quickly locate the position of the crosshair positioning mark line 16. By using the crosshair positioning mark line 16, which is set on the same straight line as the central axis of counting chamber 6, you can more quickly locate the center position of counting chamber 6. Under the guidance of the crosshair positioning mark line 16, you can finely adjust the stage in the horizontal direction to accurately align with the center area of the corresponding counting chamber. Then, switch to a high-power microscope to directly observe the grid and cells without repeatedly moving the stage or focusing to find the counting area, thus improving observation efficiency. This is especially suitable for novice operation and high-throughput counting scenarios.
[0027] The cross-shaped positioning mark line 16 is set above the two side support platforms 5, and there are four sets in total. Each counting chamber 6 corresponds to two sets. The two sets of cross-shaped positioning mark lines 16 corresponding to a single counting chamber 6 are located on the same straight line of a central axis of the counting chamber 6 and are equidistant from the center position of the counting chamber 6. The cross-shaped positioning mark line 16 is a recessed design with a groove width of 0.5 mm.
[0028] When observing counting chamber 6, you can first use a low-power microscope to quickly locate the position of the crosshair positioning mark line 16. By setting the crosshair positioning mark line 16 on the same straight line as the central axis of counting chamber 6, you can more quickly locate the center position of counting chamber 6. Under the guidance of the crosshair positioning mark line 16, you can finely adjust the stage in the horizontal direction to accurately align with the center area of the corresponding counting chamber. Then switch to a high-power microscope to directly observe the grid and cells without repeatedly moving the stage or focusing to find the counting area, which improves the observation efficiency and is especially suitable for novice operation and high-throughput counting scenarios.
[0029] The protective film 10 is provided with easy-tear lines 17, which are distributed along the edge of the dust-free paper 11.
[0030] After removing the protective film 10, tear off the part of the protective film 10 outside the edge of the lint-free paper 11 along the easy-tear line 17. Place the lint-free paper 11 face down and gently insert it into the overflow tank 2. This will absorb the excess liquid in the overflow tank 2 and the separating groove 3, eliminating the need to take out the lint-free paper 11 separately and improving the efficiency of operation.
[0031] One end of the protective film 10 is connected to a peeler 12.
[0032] Peeling tab 12 is used to easily peel off the protective film.
[0033] The cover glass positioning seat 7 is provided with a snap-fit receiving groove on its upper part. Inside the snap-fit receiving groove is a snap-fit 8 integrally formed with the cover glass positioning seat 7. The snap-fit 8 snaps onto the top edge of the cover glass 9.
[0034] The cover glass positioning seat 7 is used to position the four corners of the cover glass 9, so that the cover glass 9 is stably clamped between the four cover glass positioning seats 7. The buckle 8 is used to fix the cover glass 9. The buckle 8 is elastic. By prying the buckle 8, it can release the fixation of the cover glass 9 and remove the cover glass 9.
[0035] An overflow prevention barrier 13 is provided above the edge of the plate 1.
[0036] During the sample addition process, excess liquid flows into the overflow tank 2 and the partition groove 3, and will not overflow to the outside of the plate 1 under the blocking effect of the overflow prevention barrier 13.
[0037] The top surface of the central support platform 4 is 0.1 mm lower than the top surface of the two side support platforms 5.
[0038] The top surface of the central support platform 4 is 0.1 mm lower than the top surface of the two side support platforms 5, so that the bottom of the coverslip and the central support platform 4 form a certain gap, allowing the cell suspension to enter the counting chamber 6.
[0039] The working principle and usage procedure of this utility model are as follows: When using this device, remove it from the packaging and place it flat on a table. Peel off the protective film 10 using the peeler 12. The cell suspension can then be quantitatively extracted from the container using a pipette. Position the pipette tip below the coverslip 9 at the junction of the counting chamber 6 and the overflow trough 2, and drip the cell suspension into it. The liquid will automatically spread through capillary action. The spreading of the liquid can be observed through the coverslip 9. The liquid should automatically fill the counting chamber along the surface of the overflow trough 2 and the central support platform 4. 6. There are no gaps or obvious air bubbles. Excess liquid flows into the overflow tank 2 and the separating groove 3. Under the protection of the overflow barrier 13, it will not overflow to the outside of the plate 1. Then, along the tear line 17, tear off the portion of the protective film 10 outside the edge of the lint-free paper 11. Place the lint-free paper 11 face down and gently insert it into the overflow tank 2 to absorb excess liquid in the overflow tank 2 and the separating groove 3. Then, transfer the device to the microscope stage. During transfer, use your index finger and thumb to contact the U-shaped positioning groove 14. When picking up this device, the U-shaped positioning groove 14 fits the finger better, increasing the contact area and making it easier to apply force. At the same time, the anti-slip texture 15 increases the friction between the U-shaped positioning groove 14 and the operator's fingers, making it easier and more stable to pick up and move the device from the table. After placing the device on the microscope stage, the positioning clamp can be inserted into the inside of the U-shaped positioning groove 14 to hold the device more securely and prevent slippage. When observing the counting chamber 6, you can first use a low magnification lens to quickly find the position of the crosshair positioning mark line 16. By using the crosshair positioning mark line 16, which is set on the same straight line as the central axis of the counting chamber 6, you can more quickly locate the center position of the counting chamber 6. Under the guidance of the crosshair positioning mark line 16, you can finely adjust the stage in the horizontal direction to accurately align with the center area of the corresponding counting chamber. Then switch to a high magnification lens to directly observe the grid and cells without repeatedly moving the stage or focusing to find the counting area, improving observation efficiency. This is especially suitable for novice operation and high-throughput counting scenarios.
[0040] The circuits, electronic components, and modules involved are all existing technologies, which can be fully implemented by those skilled in the art, and need not be elaborated upon. The content protected by this application does not involve any improvement to the software and methods.
[0041] It should be noted that, in this document, the terms “comprising,” “including,” or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.
[0042] This article uses specific examples to illustrate the principles and implementation methods of this utility model. The above examples are only for the purpose of helping to understand the method and core ideas of this utility model. The above description is only a preferred embodiment of this utility model. It should be noted that due to the limitations of textual expression, there are objectively infinite specific structures. For those skilled in the art, several improvements, modifications, or changes can be made without departing from the principles of this utility model, and the above technical features can also be combined in an appropriate manner. These improvements, modifications, changes, or combinations, or the direct application of the concept and technical solution of the utility model to other occasions without modification, should all be considered within the protection scope of this utility model.
Claims
1. A disposable cell counting plate, characterized in that: The system includes a plate (1), on the top of which are provided four symmetrical overflow troughs (2). A two-sided support platform (5) is formed between the two overflow troughs (2) on the left and between the two overflow troughs (2) on the right. A central support platform (4) is formed between the two overflow troughs (2) in the middle. A dividing groove (3) is provided downwards in the middle of the central support platform (4). The two ends of the dividing groove (3) are respectively connected to the overflow troughs (2) on both sides of the central support platform (4). The portion of the central support platform (4) located on both sides of the dividing groove (3) is provided with a measuring device. The central support platform (4) of the room (6) is provided with four sets of cover glass positioning seats (7) on the top. The four sets of cover glass positioning seats (7) are symmetrically distributed in the left, right and front and back. Cover glass (9) is placed between the four sets of cover glass positioning seats (7). A protective film (10) is attached to the top of the cover glass (9). A dust-free paper (11) is attached to the top of the protective film (10). Both ends of the plate (1) are provided with U-shaped positioning grooves (14). The inner side of the U-shaped positioning grooves (14) is provided with anti-slip textures (15). The top of the two side support platforms (5) is provided with cross-shaped positioning marking lines (16).
2. The disposable cell counting plate according to claim 1, characterized in that: The cross-shaped positioning mark line (16) is set above the two side support platforms (5), and there are four sets in total. Each counting chamber (6) corresponds to two sets. The two sets of cross-shaped positioning mark lines (16) corresponding to a single counting chamber (6) are located on the same straight line of a central axis of the counting chamber (6) and are equidistant from the center of the counting chamber (6). The cross-shaped positioning mark line (16) is a recessed design with a groove width of 0.5 mm.
3. The disposable cell counting plate according to claim 1, characterized in that: The protective film (10) is provided with an easy-tear line (17), which is distributed along the edge of the dust-free paper (11).
4. A disposable cell counting chamber according to claim 1, characterized in that: One end of the protective film (10) is connected to a peeler (12).
5. A disposable cell counting plate according to claim 1, characterized in that: The cover glass positioning seat (7) is provided with a buckle receiving groove on its upper part. The buckle receiving groove is provided with a buckle (8) integrally formed with the cover glass positioning seat (7). The buckle (8) fastens the top edge of the cover glass (9).
6. A disposable cell counting plate according to claim 1, characterized in that: An overflow prevention barrier (13) is provided above the edge of the plate (1).
7. A disposable cell counting plate according to claim 1, characterized in that: The top surface height of the central support platform (4) is 0.1 mm lower than the top surface height of the two side support platforms (5).