An immune cell identification flow cytometer

Abstract

The application discloses an immune cell identification flow cytometer, relates to the technical field of cell identification, and comprises a conveying assembly, the conveying assembly comprising a bottom plate A, the top of the bottom plate A being symmetrically rotationally connected with a limiting column A, the outer circumferential surface of the limiting column A being rotationally connected with a conveying belt, the conveying belt being rotationally connected with a limiting column B, the limiting column B being rotationally connected with a bottom plate B, equidistant mounting holes being formed in the conveying belt, a fixing assembly being arranged on the mounting holes, the fixing assembly comprising a plug rod which is plugged into the mounting holes, and the top end of the plug rod being fixedly connected with a mounting seat, according to the position of a detection port, the position of the adjusting block is driven to move by adjusting the threaded column B, so that the position of the positioning plate is adjusted, no matter the size of the detection port of the cell instrument main body, the test tube at the top of the mounting seat can be accurately positioned vertically below the detection port through the adjusting steps, and the problem that the adaptability of the conveying device to the detection port of different cell instrument main bodies is poor is solved.

Description

Technical Field

[0001] This application relates to the field of cell identification technology, and in particular to a flow cytometer for identifying immune cells. Background Technology

[0002] Flow cytometers are advanced biomedical analytical instruments that integrate multiple high technologies such as optoelectronics, laser technology, fluorescence chemistry, monoclonal antibody technology, and computer technology. Currently, automated flow cytometers require the instrument to be in standby mode when the test tube is changed, resulting in low work efficiency. Therefore, some flow cytometers use test tube delivery devices to improve work efficiency.

[0003] Furthermore, the existing Chinese patent publication number CN207923675U discloses a flow cytometer, which has the following shortcomings in practical use:

[0004] The device uses a base plate added below the main body of the cell analyzer, and a delivery device for transporting test tubes is mounted on the base plate. However, in actual application scenarios, this delivery device is not compatible with most cell analyzer bodies, and the operating rhythm of the delivery device and the cell analyzer body is not consistent during subsequent use, which seriously affects the overall operating efficiency and stability of the equipment. Therefore, this solution proposes an immune cell identification flow cytometer. Utility Model Content

[0005] To address the issue of poor adaptability of test tube delivery devices, this application provides a flow cytometer for immune cell identification.

[0006] The flow cytometer for identifying immune cells provided in this application adopts the following technical solution:

[0007] An immune cell identification flow cytometer includes a main body of the cytometer, a connecting assembly at the detection end of the main body, a transport assembly on one side of the connecting assembly, and a positioning assembly on one side of the transport assembly. The transport assembly includes a base plate A, with limiting posts A symmetrically rotatably connected to the top of the base plate A. A conveyor belt is rotatably connected to the outer peripheral surface of the limiting posts A, and the conveyor belt is rotatably connected to limiting posts B. The limiting posts B are rotatably connected to the base plate B. Mounting holes are equidistantly opened on the conveyor belt, and fixing assemblies are provided in the mounting holes. The fixing assemblies include inserts into the mounting holes, and mounting seats are fixedly connected to the top ends of the inserts. Limiting posts C are equidistantly movably passed through the outer peripheral surface of the mounting seats. Clamping blocks are fixedly connected to one end of the multiple limiting posts C, and compression springs B are sleeved on the outer peripheral surfaces of the multiple limiting posts C, with their two ends respectively abutting against the clamping blocks and the mounting seats.

[0008] By adopting the above technical solution, the installation position of the conveying component can be determined by connecting components, thus achieving precise positioning of the conveying device. Then, the conveying device can be fixed by base plate A and base plate B, which can enhance the stability of the conveying device. After the position of the conveying device is adjusted, the fixing components can be placed on the conveying device as needed, increasing the flexibility of the device.

[0009] Preferably, a suction cup B is provided on one side of the bottom of the base plate A, and a suction cup C is provided on one side of the bottom of the base plate B.

[0010] By adopting the above technical solution, the installation of suction cups B and C can serve to fix base plate A and base plate B.

[0011] Preferably, the outer peripheral surfaces of both the limiting post A and the limiting post B are provided with toothed grooves, and a turntable is fixedly connected to the end of one of the limiting posts B.

[0012] By adopting the above technical solution, the installation of the turntable can play the role of flexibly controlling the conveying device. The toothed grooves opened on the outer peripheral surfaces of limit post A and limit post B can increase the friction and improve the connection stability.

[0013] Preferably, the inner surfaces of the clamping blocks are all curved.

[0014] By adopting the above technical solution, the inner surface of the clamping block is curved, which can make the surface fit of the test tube better and make the test tube more stable when fixed.

[0015] Preferably, the connecting assembly includes a vertical plate that fits into the main body of the cell analyzer, a circular groove is provided in the middle of the outer side of the vertical plate, a suction cup A is fixedly installed in the middle of the bottom of the circular groove, and a horizontal plate is fixedly connected to the lower outer side of the vertical plate.

[0016] By adopting the above technical solution, the installation of suction cup A can fix the vertical plate to the main body of the cell analyzer.

[0017] Preferably, a connecting plate A is slidably connected to the bottom of the horizontal plate, a groove is provided on the side of the connecting plate A, and a threaded post A is threadedly connected to the side of the horizontal plate, with a positioning block movably passing through the threaded post A.

[0018] By adopting the above technical solution and adjusting the threaded column A, the positioning block can be driven to cooperate with the horizontal plate to clamp and fix it on the connecting plate A.

[0019] Preferably, the positioning component includes a connecting plate B fixedly connected to the base plate A, a fixing block symmetrically fixedly connected to the top of the connecting plate B, a threaded post B movably passing through the two fixing blocks, and an adjusting block threadedly connected to the two threaded posts B.

[0020] By adopting the above technical solution, rotating the threaded column B can horizontally adjust the lateral position of the adjusting block, thereby achieving the adjustment function.

[0021] Preferably, a limiting plate is fixedly connected to the middle of the inner side of the adjusting block, a limiting rod is fixedly connected to one end of the limiting plate, a positioning plate is hinged to the upper side of one side of the inner side of the adjusting block, a limiting groove is opened on the positioning plate and moves through the limiting rod, a limiting disk is fixedly connected to the end of the limiting rod, and a compression spring A is sleeved on the outer circumferential surface of the limiting rod for abutting the limiting disk and the positioning plate at both ends.

[0022] By adopting the above technical solution, the positioning plate is always fixed in the same position under the action of the compression spring A, thereby playing a positioning role.

[0023] In summary, this application includes at least one of the following beneficial technical effects:

[0024] Based on the position of the detection port, adjust the adsorption position of the vertical plate on the side surface of the cell analyzer body, and fix it with threaded post A and positioning block to quickly complete the position adjustment of the connecting components. According to the position of the detection port, adjust the threaded post B to drive the adjustment block to move, thereby completing the position adjustment of the positioning plate. Regardless of the size of the detection port of the cell analyzer body, these adjustment steps can ensure that the test tube on the top of the mounting base is accurately positioned vertically below the detection port, thus solving the problem of poor compatibility between the delivery device and different cell analyzer body detection ports. Attached Figure Description

[0025] Figure 1 This is a schematic diagram of the overall top view of this application document;

[0026] Figure 2 This is a schematic diagram of the overall side view structure of this application document;

[0027] Figure 3 This is a schematic diagram of the transport component structure in this application.

[0028] Figure 4 This is a schematic diagram of the positioning component structure in this application.

[0029] Figure 5 This is a schematic diagram of the fixed component structure of this application document;

[0030] Figure 6 This is a schematic diagram of the transport component structure in this application.

[0031] Figure 7 This is a schematic diagram of the connection component structure in this application document;

[0032] Figure 8 This is a schematic diagram of the fixed component structure of this application.

[0033] Reference numerals: 1. Cell analyzer body; 2. Connecting assembly; 201. Vertical plate; 202. Circular groove; 203. Suction cup A; 204. Horizontal plate; 205. Connecting plate A; 2050. Slide groove; 206. Threaded column A; 207. Positioning block;

[0034] 3. Positioning assembly; 300. Connecting plate B; 301. Fixing block; 302. Threaded post B; 303. Adjusting block; 304. Limiting plate; 305. Limiting rod; 306. Positioning plate; 3060. Limiting groove; 307. Compression spring A; 308. Limiting disc;

[0035] 4. Conveying assembly; 401. Base plate A; 4010. Suction cup B; 402. Limiting post A; 403. Conveyor belt; 4030. Mounting hole; 404. Limiting post B; 405. Base plate B; 4050. Suction cup C; 406. Turntable;

[0036] 5. Fixing components; 501. Mounting base; 502. Limiting post C; 503. Compression spring B; 504. Clamping block; 505. Insert rod. Detailed Implementation

[0037] The following is in conjunction with the appendix Figures 1-8 This application will be described in further detail.

[0038] The device's "up, down, left, right" perspectives are... Figure 1 The orientation of the attached diagram is the reference.

[0039] This application discloses a flow cytometer for identifying immune cells.

[0040] Reference Figure 1 , Figure 3 , Figure 5 , Figure 8As shown, a flow cytometer for immune cell identification includes a cell analyzer body 1. A connecting component 2 is provided at the detection end of the cell analyzer body 1. A transport component 4 is provided on one side of the connecting component 2, and a positioning component 3 is provided on one side of the transport component 4. The transport component 4 includes a base plate A401 located below the detection port of the cell analyzer body 1. The top of the base plate A401 has symmetrically formed circular grooves with limiting grooves. A limiting post A402 with toothed grooves on its outer surface is rotatably connected to the circular groove. A conveyor belt 403 with mounting holes 4030 evenly spaced on its surface is rotatably connected to the outer peripheral surface of the limiting post A402. A limiting post B404 with toothed grooves on its outer surface is rotatably connected to the conveyor belt 403. The limiting post B404 is rotatably connected in the limiting circular groove of the base plate B405. A fixing component 5 is provided on the mounting hole 4030. The fixing component 5 includes... The mounting base 501 includes a rod 505 inserted into the mounting hole 4030. The top end of the rod 505 is fixedly connected to the middle of the bottom of the mounting base 501. The outer circumferential surface of the mounting base 501 is provided with circular holes at equal intervals. Limiting posts C502 are movably passed through the circular holes. One end of the multiple limiting posts C502 is fixedly connected to a clamping block 504 with an arc-shaped end. A compression spring B503 is sleeved on the outer circumferential surface of the multiple limiting posts C502. One end of the compression spring B503 abuts against the clamping block 504, and the end of the compression spring B503 away from the clamping block 504 abuts against the mounting base 501. A circular groove is provided on one side of the bottom of the base plate A401. A suction cup B4010 is fixedly installed in the circular groove. A circular groove is provided on one side of the bottom of the base plate B405. A suction cup C4050 is fixedly installed in the circular groove. A turntable 406 is fixedly connected to the end of one of the limiting posts B404.

[0041] Now, install the connecting component 2 to determine the position of the conveying component 4, thereby fixing the base plate A401. Then, the conveyor belt 403 is fitted onto the outer peripheral surface of the limiting post A402, and then onto the outer peripheral surface of another set of limiting posts B404. By pulling the base plate B405, the conveyor belt 403 is taut, thus completing the positioning of the conveying component 4. Finally, by pressing the base plate A401 and the base plate B405, the suction cup B4010 and the suction cup C405 are engaged. The 0 is adsorbed on the operating table, thus completing the fixation of the conveyor component 4. Finally, the required fixing component 5 is placed in the mounting hole 4030 on the conveyor belt 403, and the insertion rod 505 is inserted into the mounting hole 4030, so that the mounting base 501 is installed on the conveyor belt 403. Then, the test tube to be identified is placed in the top center of the mounting base 501. By pressing the test tube, the outer peripheral surface of the test tube abuts against the inner side of the clamping block 504, so that the test tube can be fixed in the top center of the mounting base 501.

[0042] Reference Figure 3 , Figure 7As shown, the connecting assembly 2 includes a vertical plate 201 that fits into the main body 1 of the cell analyzer. A circular groove 202 is provided in the middle of the outer side of the vertical plate 201. A suction cup A203 is fixedly installed in the middle of the outer side of the vertical plate 201 at the circular groove 202. A horizontal plate 204 with a side surface flush with the vertical plate 201 is fixedly connected to the lower part of the outer side of the vertical plate 201. A connecting plate A205 that is fixedly connected to the bottom plate A401 is slidably connected to the bottom of the horizontal plate 204. A sliding groove 2050 for limiting is provided on the side of the connecting plate A205. A threaded hole is provided on the side of the horizontal plate 204. A threaded post A206 is threadedly connected to the threaded hole. A positioning block 207 that is slidably connected to the sliding groove 2050 is movably passed through the threaded post A206.

[0043] First, based on the position of the detection port of the cell analyzer body 1, adjust the adsorption position of the vertical plate 201 on the side surface of the cell analyzer body 1. Once the position is determined, press the vertical plate 201 to allow the suction cup A203 to adhere to the cell analyzer body 1. Then, adjust the position of the connecting plate A205 below the horizontal plate 204 to change the position of the base plate A401. Once the position is determined, rotate the threaded column A206 to move the positioning block 207 inward, causing the positioning block 207 to press against the side surface of the connecting plate A205, thereby fixing the horizontal plate 204 above the connecting plate A205, thus quickly completing the adjustment of the position of the connecting component 2.

[0044] Reference Figure 2 , Figure 4 As shown, the positioning assembly 3 includes a connecting plate B300 fixedly connected to the base plate A401. Two fixing blocks 301 with circular holes on their side surfaces are symmetrically fixedly connected to the top side of the connecting plate B300. Threaded posts B302 are movably inserted through the circular holes in the two fixing blocks 301. An adjusting head is fixedly connected to one end of each threaded post B302. Adjusting blocks 303, whose bottoms slide against the top surface of the connecting plate B300, are threadedly connected to the two threaded posts B302. A positioning plate 306, for contacting the side surface of the mounting base 501, is hinged to the upper part of one side of the adjusting block 303. A limiting groove 3 is provided on the positioning plate 306. 060, a limiting plate 304 is fixedly connected to the inner side of the adjusting block 303 near the positioning plate 306. A limiting rod 305 is fixedly connected to one end of the limiting plate 304 and moves through the limiting groove 3060. The limiting rod 305 is composed of two columns of different diameters welded together. A limiting disc 308 with a diameter larger than that of the limiting rod 305 is fixedly connected to the end of the limiting rod 305. A compression spring A307 is sleeved on the outer circumferential surface of the limiting rod 305. One end of the compression spring A307 abuts against the limiting disc 308, and the end of the compression spring A307 away from the limiting disc 308 abuts against the positioning plate 306.

[0045] Based on the position of the detection port of the cell analyzer body 1, the threaded column B302 is adjusted, thereby driving the adjusting block 303 to move horizontally on the connecting plate B300, which in turn moves the positioning plate 306 to the side vertically below the detection port, thus completing the position adjustment of the positioning plate 306. When the mounting base 501 moves on the conveyor belt 403, after the side surface of the mounting base 501 is in contact with the side surface of the positioning plate 306, the test tube located at the top of the mounting base 501 is exactly located vertically below the detection port of the cell analyzer body 1, thus achieving precise positioning. After the detection is completed, by rotating the turntable 406, the conveyor belt 403 continues to move, which in turn moves the mounting base 501, which will squeeze the positioning plate 306, which will squeeze the compression spring A307, causing the positioning plate 306 to shift at an angle. After reaching a certain angle, the positioning plate 306 will disengage from the mounting base 501, and the positioning plate 306 will return to its original position under the compression spring A307.

[0046] The implementation principle of the flow cytometer for immune cell identification in this application embodiment is as follows: First, install the connecting component 2 to determine the position of the delivery component 4. Then, fix the base plate A401, and sequentially attach the conveyor belt 403 to the outer peripheral surfaces of the limiting post A402 and another set of limiting posts B404. Pull the base plate B405 to straighten the conveyor belt 403, completing the positioning of the delivery component 4. Press the base plate A401 and the base plate B405 to make the suction cup B4010 and the suction cup C4050 adhere to the operating table, realizing... To fix the conveyor assembly 4, place the required fixing assembly 5 into the mounting hole 4030 of the conveyor belt 403, insert the insertion rod 505 into the mounting hole 4030, so that the mounting base 501 is mounted on the conveyor belt 403, place the test tube to be identified in the middle of the top of the mounting base 501, press the test tube so that the outer circumferential surface of the test tube abuts against the inner side of the clamping block 504, thereby fixing the test tube firmly in the middle of the top of the mounting base 501 so that it can be transported to the detection port of the cell analyzer body 1 for detection via the conveyor belt 403.

[0047] The above are merely optional embodiments of this disclosure and are not intended to limit this disclosure. Various modifications and variations can be made to this disclosure by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this disclosure should be included within the scope of protection of this disclosure.

Claims

1. A flow cytometer for identifying immune cells, comprising a main body (1), characterized in that: A connecting component (2) is provided at the detection end of the main body (1) of the cell analyzer. A conveying component (4) is provided on one side of the connecting component (2). A positioning component (3) is provided on one side of the conveying component (4). The conveying component (4) includes a base plate A (401). A limiting post A (402) is symmetrically and rotatably connected to the top of the base plate A (401). A conveyor belt (403) is rotatably connected to the outer peripheral surface of the limiting post A (402). A limiting post B (404) is rotatably connected to the conveyor belt (403). A base plate B (405) is rotatably connected to the limiting post B (404). Mounting holes (4030) are equidistantly opened on the conveyor belt (403). A fixing component (5) is provided on the mounting hole (4030). The fixing component (5) includes a plug rod (505) inserted into the mounting hole (4030). The top end of the plug rod (505) is fixedly connected to a mounting base (501). The outer peripheral surface of the mounting base (501) is equidistantly connected to limit posts C (502). One end of the multiple limit posts C (502) is fixedly connected to a clamping block (504). The outer peripheral surface of the multiple limit posts C (502) is sleeved with a compression spring B (503) for abutting the clamping block (504) and the mounting base (501) at both ends.

2. The flow cytometer for identifying immune cells according to claim 1, characterized in that: A suction cup B (4010) is provided on one side of the bottom of the base plate A (401), and a suction cup C (4050) is provided on one side of the bottom of the base plate B (405).

3. The flow cytometer for identifying immune cells according to claim 1, characterized in that: The outer peripheral surfaces of the limiting post A (402) and the limiting post B (404) are provided with toothed grooves, and a turntable (406) is fixedly connected to the end of the limiting post B (404).

4. The flow cytometer for identifying immune cells according to claim 1, characterized in that: The inner surfaces of the clamping blocks (504) are all curved.

5. The flow cytometer for identifying immune cells according to claim 1, characterized in that: The connecting component (2) includes a vertical plate (201) that fits into the main body (1) of the cell instrument. A circular groove (202) is provided in the middle of the outer side of the vertical plate (201). A suction cup A (203) is fixedly installed in the middle of the bottom of the circular groove (202). A horizontal plate (204) is fixedly connected to the lower part of the outer side of the vertical plate (201).

6. The flow cytometer for identifying immune cells according to claim 5, characterized in that: The bottom of the horizontal plate (204) is slidably connected to a connecting plate A (205), and a groove (2050) is provided on the side of the connecting plate A (205). The side of the horizontal plate (204) is threadedly connected to a threaded post A (206), and a positioning block (207) is movably passed through the threaded post A (206).

7. The flow cytometer for identifying immune cells according to claim 1, characterized in that: The positioning component (3) includes a connecting plate B (300) fixedly connected to the base plate A (401). The top of the connecting plate B (300) is symmetrically fixedly connected to a fixing block (301). The two fixing blocks (301) are movably connected through a threaded post B (302). The two threaded posts B (302) are threadedly connected to an adjusting block (303).

8. The flow cytometer for identifying immune cells according to claim 7, characterized in that: A limiting plate (304) is fixedly connected to the middle of the inner side of the adjusting block (303). A limiting rod (305) is fixedly connected to one end of the limiting plate (304). A positioning plate (306) is hinged to the upper side of one side of the inner side of the adjusting block (303). A limiting groove (3060) is provided on the positioning plate (306) and moves through the limiting rod (305). A limiting disk (308) is fixedly connected to the end of the limiting rod (305). A compression spring A (307) is sleeved on the outer peripheral surface of the limiting rod (305) for abutting the limiting disk (308) and the positioning plate (306) at both ends.

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