A multi-antibody immunohistochemistry detection plate

Abstract

The utility model relates to detection board technical field, and disclose a kind of multi-antibody immunohistochemical detection board, including the detection board main part of upper portion being open, two symmetrical clamping strips are equipped in detection board main part, glass slide is equipped between two clamping strips, cover glass is equipped in glass slide upper portion, two clamping strips are tightly in glass slide and cover glass opposite side, relative side wall in detection board main part is equipped with several elastic sheets with elasticity, clamping strip is equipped in elastic sheet side, the upper portion of detection board main part opposite side is equipped with several semicircular threaded columns, and semicircular threaded column side between two adjacent detection board main body is threadedly connected with locking ring. In the utility model, one glass slide is separately placed on each detection board main body, and the number of detection board main body can be connected and fixed according to the number of samples to be detected, to improve the accuracy when immunohistochemical detection of various antibody samples.

Description

Technical Field

[0001] This utility model relates to the field of detection plate technology, specifically a multi-antibody immunohistochemical detection plate. Background Technology

[0002] Immunohistochemistry is an indispensable key technology in pathological diagnosis and biomedical research. It uses antigen-antibody specific reactions to locate and qualitatively analyze target proteins in tissue sections. With the development of precision medicine, the demand for multiple target detection of the same tissue sample is increasing, in order to obtain richer information on protein co-expression, cell typing and interaction in the morphological background.

[0003] A search revealed a patent, CN212808309U, which discloses a multi-antibody immunohistochemical detection plate. This plate comprises a multi-well plate and a glass slide. The multi-well plate has a longitudinal groove with one open end on its bottom surface, into which the glass slide is inserted. The multi-well plate also has multiple through-holes. This invention enables simultaneous detection of multiple antibodies on the same glass slide, achieving high-throughput immunohistochemical detection. It significantly improves upon the extremely low success rate of developing immunohistochemical in vitro diagnostic reagent-type monoclonal antibodies, demonstrating promising application prospects.

[0004] The aforementioned patents have significant beneficial effects, but in practical application, they still have the following shortcomings: In practical applications, when performing immunohistochemical detection on multiple antibody samples, a single slide can only perform immunohistochemical detection on a single or single type of multiple antibody sample. However, the aforementioned comparative documents involve loading multiple or more multiple antibody samples onto a single slide. When multiple or more antibody samples are stored on a single slide, cross-reactions are likely to occur, reducing the accuracy of immunohistochemical detection of multiple antibody samples. Therefore, there is an urgent need in the field to improve the multi-antibody immunohistochemical detection plate to overcome the shortcomings of the existing technology. Utility Model Content

[0005] To address the shortcomings of existing technologies, this invention provides a multi-antibody immunohistochemical detection plate, which improves the accuracy of immunohistochemical detection of multiple antibody samples.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a multi-antibody immunohistochemical detection plate, comprising a detection plate body with an open upper part, two symmetrical clamping strips inside the detection plate body, a glass slide between the two clamping strips, a cover glass slide on the upper part of the glass slide, the two clamping strips closely abutting the opposite sides of the glass slide and the cover glass slide, several elastic sheets on opposite sidewalls inside the detection plate body, the clamping strips being located on one side of the elastic sheets, several semi-circular threaded posts on the upper part of opposite sidewalls of the detection plate body, and a locking ring threadedly connecting the sides of two symmetrical semi-circular threaded posts on two adjacent detection plate bodies.

[0007] Preferably, the detection plate body has several matching positioning posts and positioning grooves on opposite sides.

[0008] Preferably, a recessed groove is provided on the side of the main body of the detection plate, the semi-circular threaded post is provided at the bottom of the recessed groove, and a sealing cover plate is provided in the recessed groove.

[0009] Preferably, the upper part of the sealing cover is provided with a rotating block, which is polygonal in shape.

[0010] Preferably, the detection plate body has pick-up and drop-off openings on its relatively short sides.

[0011] Preferably, a first insertion block is fixedly installed on the side of the elastic sheet near the clamping bar, and a first insertion groove adapted to the first insertion block is opened on one side of the clamping bar.

[0012] Preferably, a second plug-in block is fixedly installed at both ends of the elastic sheet, and a second plug-in groove adapted to the second plug-in block is opened on the inner side wall of the detection plate body.

[0013] Preferably, the upper side of the clamping bar is provided with an inclined guide angle.

[0014] Preferably, the detection plate body has card interfaces on its relatively short sides that are adapted to both ends of the cover glass, and the card interfaces are connected to the pick-and-place ports.

[0015] To address the shortcomings of existing technologies, this invention provides a multi-antibody immunohistochemical detection plate, overcoming these deficiencies. The beneficial effects of this invention are as follows: In this invention, each detection plate has a separate glass slide placed on it, and the number of detection plates can be connected and fixed according to the number of samples to be tested, thereby improving the accuracy of immunohistochemical detection of multiple antibody samples.

[0016] In this invention, the positioning pins on the sides of two adjacent detection plate bodies are inserted into the positioning grooves, which improves the stability of the two detection plate bodies after connection. The locking rings are threadedly connected to the sides of two adjacent semi-circular threaded pins, which facilitates the connection or disassembly of the two adjacent detection plate bodies.

[0017] In this invention, a first insert block on one side of the elastic sheet is inserted into a first insert groove on the side of the clamping strip, and second insert blocks at both ends of the elastic sheet are inserted into second insert grooves. This allows for the replacement of the clamping strip and the elastic sheet, and enables the limiting and fixing of glass slides of different sizes, thereby improving the applicability of the immunohistochemical detection plate.

[0018] Other features and advantages of this invention will be set forth in the description which follows, and will be apparent in part from the description, or may be learned by practicing the invention. The objects and other advantages of this invention can be realized and obtained by means of the structures pointed out in the description, claims, and drawings. Attached Figure Description

[0019] The accompanying drawings are provided to further understand the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention and do not constitute a limitation thereof.

[0020] Figure 1 This is a schematic diagram of the structure after the two detection plates are connected in this utility model; Figure 2 This is a schematic diagram of the main body of the detection plate in this utility model; Figure 3 for Figure 2 Enlarged structural diagram at point A in the middle; Figure 4 for Figure 2 Enlarged structural diagram at point B.

[0021] In the diagram: 1. Detection plate body; 2. Clamping strip; 3. Glass slide; 4. Cover glass; 5. Elastic sheet; 6. Semi-circular threaded post; 7. Locking ring; 8. Positioning post; 9. Positioning groove; 10. Recessed groove; 11. Sealing cover plate; 12. Rotating block; 13. Pick-up and drop-out port; 14. First insertion block; 15. First insertion groove; 16. Second insertion block; 17. Second insertion groove; 18. Guide angle; 19. Snap-fit ​​interface. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0023] Example 1 Please see Figures 1-4 A multi-antibody immunohistochemical detection plate includes a detection plate body 1 with an open upper part. The detection plate body 1 has two symmetrical clamping strips 2 inside, and a glass slide 3 is placed between the two clamping strips 2. A cover glass 4 is placed on the upper part of the glass slide 3. The two clamping strips 2 are in close contact with the opposite sides of the glass slide 3 and the cover glass 4. The inner opposite sidewalls of the detection plate body 1 are provided with a number of elastic sheets 5. The clamping strips 2 are located on one side of the elastic sheets 5. The upper part of the opposite side of the detection plate body 1 is provided with a number of semi-circular threaded posts 6. The sides of two symmetrical semi-circular threaded posts 6 on two adjacent detection plate bodies 1 are threadedly connected by locking rings 7.

[0024] The specific implementation method in this embodiment is as follows: When multiple antibody samples need to be detected by immunohistochemistry, the multiple antibody samples are transferred onto multiple glass slides 3, and coverslips 4 are used to encapsulate the samples. Several detection plate bodies 1 are connected according to the number of samples to be detected. The sides of two adjacent detection plate bodies 1 are aligned, and the sides of two adjacent semi-circular threaded pillars 6 are provided with matching threads. Locking rings 7 are threaded between the sides of two adjacent semi-circular threaded pillars 6. Tightening the locking rings 7 limits and fixes the two adjacent detection plate bodies 1. The appropriate number of detection plate bodies 1 are connected in the above manner, and one detection plate body 1 is pushed in the opposite direction. Two clamping strips 2 move in opposite directions and apply pressure to the elastic sheet 5, placing the glass slide 3 between the two clamping strips 2. The two clamping strips 2 are then released, and the tension of the elastic sheet 5 pushes the clamping strips 2 tightly against the sides of the glass slide 3 and coverslip 4, thus positioning and fixing the glass slide 3 and coverslip 4. Several interconnected detection plate bodies 1 are simultaneously placed in an incubator for incubation. After incubation, immunohistochemical detection of various antibody samples is performed. Since each detection plate body 1 has a separate glass slide 3, and the number of detection plate bodies 1 can be connected and fixed according to the number of samples to be tested, the accuracy of immunohistochemical detection of various antibody samples is improved.

[0025] Example 2 Please see Figure 3This embodiment includes the above embodiment, and further includes: the detection plate body 1 is provided with several matching positioning posts 8 and positioning grooves 9 on opposite sides, the detection plate body 1 is provided with a sinking groove 10 on the side, the semi-circular threaded post 6 is provided at the bottom of the sinking groove 10, the sinking groove 10 is provided with a sealing cover plate 11, and the upper part of the sealing cover plate 11 is provided with a rotating block 12, which is polygonal in shape.

[0026] In this embodiment, when connecting two adjacent detection plate bodies 1, the positioning pins 8 on the sides of the two adjacent detection plate bodies 1 are inserted into the positioning grooves 9 to improve the stability of the two detection plate bodies 1 after connection. After the two detection plate bodies 1 are threadedly connected to the semi-circular threaded pins 6 by locking rings 7, the sealing cover 11 is threadedly connected to the two recessed grooves 10 by rotating the rotating block 12. The sealing cover 11 can prevent the locking rings 7 from loosening and improve the aesthetics of the connection of several detection plate bodies 1.

[0027] Example 3 Please see Figure 1 , Figure 3 and Figure 4 This embodiment includes all the above embodiments, and further includes: the detection plate body 1 is provided with a pick-and-place port 13 on the relatively short side, the detection plate body 1 is provided with a card interface 19 adapted to both ends of the cover glass 4 on the relatively short side, the card interface 19 is connected to the pick-and-place port 13, and the upper side of the clamping strip 2 is provided with an inclined guide angle 18.

[0028] In this embodiment, the slide 3 can be picked up and put down through the arc-shaped pick-and-place opening 13, which facilitates the picking up and putting down of the slide 3. The two ends of the cover glass 4 are snapped into the snap-fit ​​interface 19, which improves the stability of the slide 3 in the detection plate body 1. When placing the slide 3, the slide 3 is pressed into the bottom of the detection plate body 1. The guide angle 18 is pressed into the side of the slide 3, and the slide 3 presses against the two clamping strips 2, which facilitates the placement of the slide 3 into the bottom of the detection plate body 1.

[0029] Example 4 Please see Figure 3 This embodiment includes all the above embodiments, and further includes: a first plug block 14 is fixedly installed on the side of the elastic sheet 5 near the clamping bar 2, a first plug groove 15 adapted to the first plug block 14 is opened on one side of the clamping bar 2, a second plug block 16 is fixedly installed on both ends of the elastic sheet 5, and a second plug groove 17 adapted to the second plug block 16 is opened on the inner side wall of the detection plate body 1.

[0030] In this embodiment, the first insertion block 14 on one side of the elastic sheet 5 is inserted into the first insertion groove 15 on the side of the clamping strip 2, and the second insertion blocks 16 at both ends of the elastic sheet 5 are inserted into the second insertion groove 17. This allows the clamping strip 2 and the elastic sheet 5 to be replaced, and allows for the positioning and fixing of glass slides 3 of different sizes, thereby improving the applicability of the immunohistochemical detection plate.

[0031] Finally, it should be noted that in the description of this utility model, the terms "vertical," "upper," "lower," "horizontal," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0032] In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0033] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A multi-antibody immunohistochemical detection plate, comprising a detection plate body (1) with an open upper portion, characterized in that, The detection plate body (1) is provided with two symmetrical clamping strips (2), and a glass slide (3) is provided between the two clamping strips (2). A cover glass (4) is provided on the upper part of the glass slide (3). The two clamping strips (2) are closely attached to the opposite sides of the glass slide (3) and the cover glass (4). Several elastic sheets (5) are provided on the opposite sidewalls of the detection plate body (1). The clamping strips (2) are located on one side of the elastic sheets (5). Several semi-circular threaded posts (6) are provided on the upper part of the opposite side of the detection plate body (1). A locking ring (7) is threaded between the sides of two symmetrical semi-circular threaded posts (6) on two adjacent detection plate bodies (1).

2. The multi-antibody immunohistochemical detection plate according to claim 1, characterized in that, The detection plate body (1) has several matching positioning posts (8) and positioning grooves (9) on opposite sides.

3. The multi-antibody immunohistochemical detection plate according to claim 1, characterized in that, The detection plate body (1) has a recessed groove (10) on its side, the semi-circular threaded column (6) is located at the bottom of the recessed groove (10), and a sealing cover plate (11) is provided in the recessed groove (10).

4. The multi-antibody immunohistochemical detection plate according to claim 3, characterized in that, The sealing cover (11) is provided with a rotating block (12) on the upper part, and the rotating block (12) is polygonal.

5. The multi-antibody immunohistochemical detection plate according to claim 1, characterized in that, The detection plate body (1) is provided with pick-up and put-out openings (13) on the relatively short sides.

6. The multi-antibody immunohistochemical detection plate according to claim 1, characterized in that, The elastic sheet (5) is fixedly installed with a first plug-in block (14) on the side near the clamping bar (2), and a first plug-in groove (15) adapted to the first plug-in block (14) is opened on one side of the clamping bar (2).

7. The multi-antibody immunohistochemical detection plate according to claim 1, characterized in that, The elastic sheet (5) has a second plug-in block (16) fixedly installed at both ends, and the inner sidewall of the detection plate body (1) is provided with a second plug-in groove (17) that is adapted to the second plug-in block (16).

8. The multi-antibody immunohistochemical detection plate according to claim 1, characterized in that, The upper side of the clamping bar (2) is provided with an inclined guide angle (18).

9. A multi-antibody immunohistochemical detection plate according to claim 5, characterized in that, The detection plate body (1) has card interfaces (19) on its relatively short sides that are adapted to the two ends of the cover glass (4), and the card interfaces (19) are connected to the pick-and-place port (13).

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