Lily tissue culture bottle with liftable and breathable tray

Abstract

This utility model discloses a lily tissue culture bottle with a liftable and breathable tray, including a base plate and a tissue culture bottle, with a frame welded to the top center of the base plate. Beneficial effects: In use, the base plate, intermediate cylinder, and cap can be connected and sealed using corresponding magnetic rings, magnetic grooves, sealing rings, and sealing grooves. Because the multiple sets of grooves and rings are arranged in an alternating manner, the overall sealing performance is improved, preventing the intrusion of external substances. The magnetic structure design also makes loading and unloading easier and more convenient. Furthermore, the entire tissue culture bottle adopts a multi-part detachable design, allowing for quick opening and assembly during tissue culture, effectively improving the treatment effect on lily tissues and reducing damage. It is convenient to use, easy to operate, labor-saving to load and unload, and highly practical.

Description

Technical Field

[0001] This utility model relates to the field of lily tissue culture technology, specifically to a lily tissue culture bottle with a liftable and breathable tray. Background Technology

[0002] Lilies are perennial herbaceous bulbous plants belonging to the genus *Lilium* in the family Liliaceae. With their beautiful flowers, large size, and vibrant colors, lilies have always been beloved by people worldwide and are considered one of the world's most prized cut flowers. They are widely used in potted plants, flower beds, borders, and specialized gardens. Through comparative cultivation experiments, it has been found that lilies grown through tissue culture not only grow normally but also outperform wild lilies under the same conditions. Therefore, most lilies today are cultivated using tissue culture. The process of tissue culture involves first placing lily buds in tissue culture bottles, and then placing these bottles on cultivation racks in a culture room for further cultivation.

[0003] In existing technologies, when plants in tissue culture bottles need to be removed, the cap is opened from the top of the bottle to take out the plants. Since the plants are in an inverted triangular shape, this method of removal is inconvenient and may damage the integrity of the plants. It is not very practical and there is room for improvement. Utility Model Content

[0004] (a) Technical problems to be solved To address the shortcomings of existing technologies, this utility model provides a lily tissue culture bottle with a liftable and breathable tray, which has the advantages of convenient operation, labor-saving loading and unloading, and strong practicality, thereby solving the problems mentioned in the background technology.

[0005] (II) Technical Solution To achieve the advantages of convenient operation, labor-saving loading and unloading, and strong practicality, the specific technical solution adopted by this utility model is as follows: A lily tissue culture bottle with a liftable and breathable tray includes a base plate and a tissue culture bottle. A frame is welded to the top center of the base plate. Several sets of limiting grooves are formed around the inner surface of the frame. The depth of the limiting grooves gradually increases. A breathable tray is slidably connected between the limiting grooves through limiting blocks. A tissue culture bottle is placed on the top of the breathable tray. The tissue culture bottle is composed of a base plate, an intermediate cylinder, and a cap. Sealing grooves are formed around both sides of the surface of the base plate and the cap, and magnetic rings are installed around the center of the surface of each. Sealing rings are installed around both sides of the surface of both ends of the intermediate cylinder, and magnetic grooves are formed around the center of the surface of each end.

[0006] Furthermore, a vent hole is provided at the middle position of the top of the cover, a vent membrane is installed inside the vent hole, and a sealing plug is inserted inside the vent hole.

[0007] Furthermore, several sets of threaded holes are symmetrically opened on the surface of the base plate.

[0008] Furthermore, several sets of ventilation grooves are provided around the surface of the frame.

[0009] Furthermore, the surface of the breathable tray is provided with several sets of through holes.

[0010] Furthermore, the number of limiting blocks and limiting slots are the same.

[0011] Furthermore, the chassis surface is provided with grooves.

[0012] (III) Beneficial Effects Compared with the prior art, this utility model provides a lily tissue culture bottle with a height-adjustable and breathable tray, which has the following beneficial effects: In use, this invention connects and seals the base, intermediate cylinder, and cap using corresponding magnetic rings, magnetic grooves, sealing rings, and sealing grooves. The staggered arrangement of multiple grooves and rings enhances overall sealing, preventing external substances from entering. The magnetic design also makes loading and unloading easier and more convenient. Furthermore, the multi-part detachable design of the entire tissue culture bottle allows for quick opening and assembly during tissue culture, effectively improving the treatment of lily tissues and reducing damage. It offers advantages such as ease of operation, effortless loading and unloading, and high practicality. Attached Figure Description

[0013] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0014] Figure 1 This is a structural schematic diagram of a lily tissue culture bottle with a liftable and breathable tray according to an embodiment of the present utility model; Figure 2 This is a cross-sectional view of a lily tissue culture bottle with a liftable and breathable tray according to an embodiment of the present utility model; Figure 3 This is a cross-sectional view of the frame of a lily tissue culture bottle with a liftable and breathable tray according to an embodiment of the present utility model; Figure 4 This is a partial structural schematic diagram of a ventilated tray for a lily tissue culture bottle with a liftable ventilated tray according to an embodiment of the present utility model. Figure 5 This is a cross-sectional view of the base of a lily tissue culture bottle with a liftable and breathable tray according to an embodiment of the present utility model. Figure 6This is a cross-sectional view of the cap of a lily tissue culture bottle with a liftable and breathable tray according to an embodiment of the present utility model. Figure 7 This is a cross-sectional view of the middle cylinder of a lily tissue culture bottle with a liftable and breathable tray according to an embodiment of the present utility model.

[0015] In the picture: 1. Base plate; 2. Frame; 3. Ventilation groove; 4. Limiting groove; 5. Base plate; 6. Intermediate cylinder; 7. Cap; 8. Sealing plug; 9. Tissue culture bottle; 10. Magnetic suction groove; 11. Ventilation tray; 12. Sealing ring; 13. Limiting block; 14. Sealing groove; 15. Magnetic suction ring; 16. Ventilation membrane; 17. Ventilation hole. Detailed Implementation

[0016] To further illustrate the various embodiments, the present invention provides accompanying drawings, which are part of the disclosure of the present invention. These drawings are mainly used to illustrate the embodiments and can be used in conjunction with the relevant descriptions in the specification to explain the operating principles of the embodiments. With reference to these contents, those skilled in the art should be able to understand other possible implementation methods and the advantages of the present invention. The components in the figures are not drawn to scale, and similar component symbols are usually used to represent similar components.

[0017] According to an embodiment of the present invention, a lily tissue culture bottle with a liftable and breathable tray is provided.

[0018] The present invention will now be further described in conjunction with the accompanying drawings and specific embodiments, such as... Figure 1-7As shown, a lily tissue culture bottle with a liftable and breathable tray according to an embodiment of the present invention includes a base plate 1 and a tissue culture bottle 9. A frame 2 is welded to the top center of the base plate 1. Several sets of limiting grooves 4 are formed around the inner surface of the frame 2. The depth of the limiting grooves 4 gradually increases. A breathable tray 11 is slidably connected between the limiting grooves 4 by limiting blocks 13. The tissue culture bottle 9 is placed on the top of the breathable tray 11. The tissue culture bottle 9 is composed of a base plate 5, an intermediate cylinder 6, and a cap 7. Sealing grooves 14 are formed around both sides of the surface of the base plate 5 and the cap 7, and magnetic rings 15 are installed around the center of the surface of both. The ends of the intermediate cylinder 6 are sealed. The tissue culture bottle 9 has sealing rings 12 installed around both sides of its surface, and a magnetic groove 10 is opened in the middle of each surface. The tissue culture bottle 9 adopts a "three-section detachable structure". The base 5, intermediate cylinder 6, and cap 7 are all transparent high-temperature resistant plastic parts, preferably made of PC, which is suitable for tissue culture sterilization process. The annular magnetic ring 15 is fixed in the middle of the upper surface of the base 5 and the lower surface of the cap 7 by injection molding. The magnetic ring 15 is a permanent magnet. The annular magnetic groove 10 is opened in the middle of the two ends of the intermediate cylinder 6. The groove diameter matches the magnetic ring 15. The two are fitted with a gap. After the magnetic ring 15 and the magnetic groove 10 are precisely aligned, they can be quickly aligned by magnetic attraction. On both sides of the upper surface of the chassis 5 and the lower surface of the cover 7, annular sealing grooves 14 are formed. On both sides of the two ends of the intermediate cylinder 6, corresponding annular sealing rings 12 are installed. The sealing rings 12 can be made of silicone. The sealing rings 12 and the sealing grooves 14 are staggered, with the magnetic attraction structure inside and the sealing structure outside. When connected, the sealing rings 12 are embedded in the sealing grooves 14, forming a double seal. The magnetic attraction between the magnetic rings 15 and the magnetic grooves 10 ensures that the sealing rings 12 are tightly embedded in the sealing grooves 14. The staggered sealing structure blocks the intrusion of external bacteria and dust. The magnetic strength can be adjusted by using magnets of different strengths, which will not be discussed here. In short, magnetic docking requires no additional tools; during loading and unloading, only pulling force is needed for separation, which is less effort than traditional threaded connections and avoids sealing failure caused by thread wear. The three-section structure can be disassembled individually; for example, only the cap 7 needs to be removed when adding culture medium, and the middle cylinder 6 can be disassembled when removing lily tissue, without the need for overall tilting. The tissue damage rate is lower than that of traditional methods, and the operational flexibility is improved. The double sealing structure prevents the intrusion of external contaminants, adapts to the requirements of aseptic tissue culture, and reduces the contamination rate of lily tissue. Magnetic quick docking makes loading and unloading more convenient, and the three-section detachable design prevents damage to lily tissue due to tilting and pulling, especially suitable for the culture of fragile buds and callus tissue.

[0019] Please refer to Figure 1 , Figure 2 and Figure 6As shown, a vent 17 is provided at the center of the top of the cover 7. A breathable membrane 16 is installed inside the vent 17, and a sealing plug 8 is inserted inside the vent 17. A circular vent 17 is provided at the center of the top of the cover 7. The breathable membrane 16 is fixed inside the vent 17 with glue, which can filter bacteria and dust, and allow air and carbon dioxide to pass through. The sealing plug 8 is made of silicone and fits the vent 17 with a gap. The sealing plug 8 can be freely inserted and removed to open and close the vent 17. The filtration precision of the breathable membrane 16 is adapted to the requirements of aseptic tissue culture, which not only ensures that gas exchange meets the respiration of lily tissue, but also prevents... It prevents contaminants from entering; the sealing plug 8 is easy to insert and remove, and the ventilation state can be controlled according to the cultivation stage, such as closing it during sterilization and opening it during cultivation, which improves flexibility; the ventilation holes 17 of the cover body 7, the through holes of the tray, and the ventilation grooves 3 of the frame body 2 form an airflow circulation of "inside the bottle - outside the bottle - frame body 2", so that the gas exchange is uniform and avoids local hypoxia; the ventilation membrane 16 realizes sterile gas exchange, and the sealing plug 8 can adjust the ventilation volume, maintaining humidity when closed and enhancing ventilation when open, which is suitable for the needs of lily tissue from callus induction to rooting; the ventilation membrane 16 can effectively block bacterial invasion, reduce the contamination rate, and avoid the gas obstruction problem caused by traditional sealing caps.

[0020] Please refer to Figure 1-3 As shown, several sets of threaded holes are symmetrically opened on the surface of the base plate 1, distributed at the four corners. The base plate 1 is fixed to the culture rack platform with bolts to prevent the rack 2 from tipping over during the culture process, which is suitable for the multi-layer culture rack environment of the culture room. The symmetrical distribution of the threaded holes ensures that the base plate 1 is subjected to uniform force. Even if multiple layers are stacked or there is a slight collision, the overall structure will not loosen, providing a stable placement environment for the tissue culture bottle 9. The base plate 1 is fixed to the culture rack through the threaded holes, which can realize the dense placement of multiple sets of devices, which is suitable for large-scale tissue culture production in the laboratory and avoids tissue culture failure caused by tipping.

[0021] Please refer to Figure 1-3 As shown, several sets of ventilation grooves 3 are arranged around the surface of the frame 2, and several sets of annular ventilation grooves 3 are arranged around the side walls of the frame 2. The ventilation grooves 3 cooperate with the through holes of the tray to form an air circulation channel. The limiting grooves 4 are set with varying depths, and the limiting blocks 13 are inserted into the limiting grooves 4 at different depths to achieve different height adjustments of the tray. The gap fit between the limiting blocks 13 and the limiting grooves 4 ensures that there is no shaking after insertion, ensuring the stability of the tissue culture bottle 9. The through holes of the tray and the ventilation grooves 3 of the frame 2 form a "through" airflow channel, which avoids liquid accumulation caused by the bottom of the tissue culture bottle 9 sticking to the tray, and promotes the exchange of gas inside the bottle with the outside, adapting to the respiration needs of lily tissue. The height of the tray can be adjusted according to the stage of lily tissue culture. For example, a higher position during the bud induction period enhances light, and a lower position during the rooting period avoids strong light, eliminating the need to replace the culture rack and improving adaptability. The through-type ventilation structure reduces the accumulation of moisture inside the bottle, preventing root rot of lily tissue, while ensuring oxygen supply and promoting growth.

[0022] Please refer to Figure 1 and Figure 4 As shown, the surface of the ventilated tray 11 has several sets of through holes. The base plate 1 is a rectangular stainless steel plate, which is corrosion-resistant and suitable for the humid environment of the culture room. The top center is fully welded with a circular frame 2, a steel ring with high rigidity, ensuring that the frame 2 is perpendicular to the base plate 1 and providing stable support for tray lifting. The inner wall of the frame 2 has several sets of limiting grooves 4 evenly distributed along the circumference, with the depth gradually increasing from bottom to top. The groove walls of the limiting grooves 4 are polished to reduce sliding friction. The ventilated tray 11 is a circular plastic tray, the same material as the tissue culture bottle 9. Several sets of limiting blocks 13 are symmetrically welded to the outer circumference, the number of which is the same as the limiting grooves 4. The limiting blocks 13 are embedded in the limiting grooves 4 to form a sliding connection. The surface of the tray has evenly distributed through holes to ensure air circulation.

[0023] Please refer to Figure 1-4 As shown, the number of limit blocks 13 and limit slots 4 are the same.

[0024] Please refer to Figure 1 , Figure 2 and Figure 5 As shown, the base 5 has a groove on its surface, and a circular groove on its upper surface. The groove size is adapted to lily tissue culture containers, such as petri dishes and culture blocks, to ensure accurate positioning of the culture after placement and to avoid damage to the lily tissue caused by shaking. Assemble the tissue culture bottle 9: Place the culture medium and lily tissue into the groove of the base 5. Align the magnetic grooves 10 at both ends of the middle cylinder 6 with the magnetic rings 15 of the base 5 and the cover 7 for adsorption and fixation. The sealing ring 12 is embedded in the sealing groove 14 to form a seal. Depending on the culture stage, choose whether to insert the sealing plug 8 to achieve the purpose of ventilation or sealing. Adjust the tray height: Slide the limiting block 13 along the limiting groove 4 of the frame 2 and insert it into the limiting groove 4 at the target depth to fix the height of the ventilated tray 11 to adapt to the light and ventilation requirements of the current tissue culture stage. Place the tissue culture bottle 9: Place the assembled tissue culture bottle 9 on the... On the ventilated tray 11, the through hole of the tray and the ventilated groove 3 of the frame 2 form an airflow channel; Fixing device: The entire device is fixed to the culture rack through the threaded hole of the base plate 1 to start the culture process; Subsequently, the tray height or the state of the vent plug can be adjusted according to the growth stage; Disassembly and handling: After the tissue culture is completed, the cap 7 and the intermediate cylinder 6 are separated, and the lily tissue is directly taken out without tilting, reducing damage; Easy operation: Magnetic disassembly + three-section structure, high loading and unloading efficiency; The tray height can be quickly adjusted without tools, and novices can get started quickly; Effortless loading and unloading: Magnetic adsorption replaces the traditional thread, making operation more effortless than the traditional tissue culture bottle 9, reducing operator fatigue; High practicality: Sealed to prevent contamination, height adjustable to adapt to multiple stages, ventilated to prevent liquid accumulation, suitable for the entire cycle of lily tissue culture, with a higher tissue culture success rate, suitable for large-scale laboratory production.

[0025] Working Principle: In practical use, when lily tissue culture is required, the culture components and lily tissue can be placed in the base plate 5. Then, the base plate 5, intermediate cylinder 6, and cap 7 are connected and sealed using corresponding magnetic rings 15, magnetic grooves 10, sealing rings 12, and sealing grooves 14. Because the multiple grooves and rings are arranged in an alternating manner, the overall sealing performance is improved, preventing the intrusion of external substances. The magnetic structure also makes loading and unloading easier and more convenient. Furthermore, the entire tissue culture bottle 9 adopts a multi-part detachable design, allowing for quick opening and assembly during tissue culture. This effectively improves the treatment effect on lily tissue and reduces damage, facilitating better processing. The assembled tissue culture bottle 9 can be placed on the ventilated tray 11. The suspended design of the ventilated tray 11 creates a gap between the entire bottle and the bottom, facilitating better gas flow, reducing the impact of liquid accumulation at the bottom, and ensuring the tissue culture effect on lilies. Moreover, at different stages of tissue culture, the ventilated tray 11 can be suspended at different heights through the limiting grooves 4 of different depths to adapt to different growth needs. For example, during the bud induction period, the tray height is adjusted to 15-20cm below the light source to enhance light exposure; during the rooting period, the tray is lowered to 20-30cm below the light source to avoid strong light inhibiting rooting, thereby improving the practicality of the structure and facilitating better use. The device as a whole has the advantages of convenient operation, easy assembly and disassembly, and high practicality.

[0026] In this utility model, unless otherwise explicitly specified and limited, the terms "installation", "setting", "connection", "fixing", "screw connection", etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal connection of two components or the interaction between two components. Unless otherwise explicitly limited, those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0027] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A lily tissue culture flask with a liftable and breathable tray, comprising a base plate (1) and a tissue culture flask (9), characterized in that, A frame (2) is welded to the top center of the base plate (1). Several sets of limiting grooves (4) are opened around the inner surface of the frame (2). The depth of the limiting grooves (4) gradually increases. A breathable tray (11) is slidably connected between the limiting grooves (4) through limiting blocks (13). A tissue culture bottle (9) is placed on the top of the breathable tray (11). The tissue culture bottle (9) is composed of a base plate (5), an intermediate cylinder (6) and a cap (7). Sealing grooves (14) are opened around both sides of the surface of the base plate (5) and the cap (7), and magnetic rings (15) are installed around the middle of the surface. Sealing rings (12) are installed around both sides of the surface of the two ends of the intermediate cylinder (6), and magnetic grooves (10) are opened in the middle of the surface.

2. The lily tissue culture bottle with a liftable and breathable tray according to claim 1, characterized in that, The cover (7) has a vent hole (17) at the middle of the top. A breathable membrane (16) is installed inside the vent hole (17), and a sealing plug (8) is inserted inside the vent hole (17).

3. The lily tissue culture bottle with a liftable and breathable tray according to claim 1, characterized in that, The base plate (1) has several sets of threaded holes symmetrically opened on its surface.

4. The lily tissue culture bottle with a liftable and breathable tray according to claim 1, characterized in that, The frame (2) has several sets of ventilation slots (3) arranged around its surface.

5. A lily tissue culture bottle with a liftable and breathable tray according to claim 1, characterized in that, The surface of the breathable tray (11) has several sets of through holes.

6. A lily tissue culture flask with a liftable and breathable tray according to claim 1, characterized in that, The number of limiting blocks (13) and limiting slots (4) are the same.

7. A lily tissue culture bottle with a liftable and breathable tray according to claim 1, characterized in that, The chassis (5) has grooves on its surface.

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