A tobacco leaf peeling device

Abstract

This utility model relates to the field of tobacco leaf testing technology, specifically a tobacco leaf epidermis tearing device, comprising movable pressure blocks and an elastic base arranged correspondingly at the top and bottom; the movable pressure block includes an adhesive layer and a first movable base, the adhesive layer being disposed on the lower surface of the first movable base, the adhesive layer being used to tear the epidermis on the back of the tobacco leaf; a fixing component is disposed on the upper end surface of the elastic base, the fixing component being used to fix the front of the tobacco leaf. This utility model can assist in tearing off the epidermis of tobacco leaves, exposing the internal protoplasts, facilitating subsequent protoplast extraction. Furthermore, the elastic design allows for adaptive adjustment of the downward pressure, avoiding damage to the tobacco leaf structure.

Description

Technical Field

[0001] This utility model relates to the field of tobacco leaf testing technology, specifically a tobacco leaf epidermis tearing device. Background Technology

[0002] Protoplasts are the naked cellular structures of plant cells after the cell wall has been removed. They are widely used in various biotechnology fields such as plant cell engineering, gene transformation, metabolic regulation, and functional gene research. Tobacco, as an important model plant and economic crop, often uses leaf protoplasts for rapid expression screening, promoter activity analysis, metabolic engineering, and functional verification of synthetic circuits.

[0003] In existing tobacco protoplast extraction processes, whole leaves are typically cut into small pieces before enzymatic hydrolysis. However, this cutting step often causes significant mechanical damage, not only disrupting some cell structures and reducing protoplast yield and activity, but also introducing a large amount of debris and impurities into the extract, affecting the efficiency of subsequent purification, transfection, and functional analysis. Furthermore, the lower epidermis of tobacco leaves possesses a certain degree of mechanical stability and acts as a shield against the enzymatic hydrolysate, potentially limiting the enzyme's contact with the inner cells and thus affecting protoplast release to some extent.

[0004] Several studies and experimental observations have shown that appropriately removing the lower epidermal structure of tobacco leaves before enzymatic hydrolysis can help improve the release efficiency and extraction purity of protoplasts. However, this operation currently relies heavily on manual processing, which suffers from cumbersome procedures, poor reproducibility, and low efficiency, especially under experimental conditions requiring large sample sizes. Therefore, there is an urgent need to design a simple, easy-to-operate tobacco leaf pretreatment device suitable for batch processing to improve protoplast extraction efficiency and support high-quality metabolic engineering and regulation-related research. Utility Model Content

[0005] The purpose of this invention is to address the shortcomings of existing technologies by providing a tobacco leaf peeling device that can easily tear open the outer skin of tobacco leaves to expose the internal protoplasts, facilitating subsequent protoplast extraction.

[0006] To achieve the above objectives, the technical solution of this utility model is as follows: A tobacco leaf peeling device includes movable pressure blocks and an elastic base arranged correspondingly at the top and bottom; The movable pressing block includes an adhesive layer and a first movable base. The adhesive layer is disposed on the lower surface of the first movable base and is used to tear the epidermis on the back of the tobacco leaf. The upper surface of the elastic base is provided with a fixing component, which is used to fix the front side of the tobacco leaf.

[0007] Furthermore, the adhesive layer is double-sided adhesive.

[0008] Furthermore, the tobacco leaf peeling device also includes a vertically arranged sliding track, on which the first movable base is slidably disposed.

[0009] Furthermore, the first movable base is driven by one of the following methods: manual drive, linear module drive, cylinder drive, electric actuator drive, or electric lead screw drive.

[0010] Furthermore, the tobacco leaf peeling device also includes a support, and the sliding track is disposed on the side of the support.

[0011] Furthermore, a flexible pressure head is provided between the first movable base and the adhesive layer.

[0012] Furthermore, the flexible pressure head is made of sponge or silicone.

[0013] Furthermore, the fixing component is double-sided adhesive.

[0014] Furthermore, the adhesiveness of the fixing component is greater than that of the adhesive layer.

[0015] Furthermore, the upper surface area of ​​the fixing component is greater than the lower surface area of ​​the adhesive layer.

[0016] Furthermore, the elastic base includes a second movable base and an elastic component. The second movable base is slidably disposed on the sliding track. One side of the second movable base is connected to the elastic component, and the other end of the elastic component is fixed to the bracket.

[0017] Furthermore, the elastic component is a spring.

[0018] Furthermore, a connecting rod is provided on one side of the second movable base, and a spring is provided above the connecting rod. The upper end of the spring is fixed to the bracket, and the lower end of the spring is connected to the connecting rod.

[0019] Compared with the prior art, the present invention has the following beneficial effects: This utility model provides a tobacco leaf peeling device that uses a movable pressure block in conjunction with an elastic base to peel the tobacco leaf by pressing and sticking, exposing the protoplasts inside. This facilitates the subsequent protoplast extraction. Compared with traditional methods such as cutting and manual removal of the leaf, this method is more efficient, provides a more thorough pretreatment, and makes protoplast extraction relatively easier.

[0020] The flexible base design allows the downward pressure to be adjusted adaptively when the downward pressure is not precisely controlled, thus avoiding damage to the tobacco leaf structure. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the structure of this utility model.

[0022] Figure 2 This is a front view of the present invention.

[0023] Figure label: 1. Movable pressing block; 2. Elastic base; 3. Tobacco leaf; 4. Support; 11. Adhesive layer; 12. First movable base; 13. Vertical track; 14. Flexible pressing head; 21. Fixing component; 22. Second movable base; 23. Spring. Detailed Implementation

[0024] The technical solution of this utility model will be clearly described below with reference to the accompanying drawings. Obviously, the described embodiments are not all embodiments of this utility model. All other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0025] It should be noted that, unless otherwise specifically stated, the relative arrangement and numerical expressions of the components and steps described in these embodiments should not be construed as limiting the scope of this utility model.

[0026] The following description of exemplary embodiments is merely illustrative and is not intended to limit the present invention or its application or use in any way. Techniques, methods, and apparatus known to those skilled in the art may not be discussed in detail herein, but where applicable, such techniques, methods, and apparatus should be considered part of this specification.

[0027] like Figure 1 and Figure 2 As shown, a tobacco leaf peeling device includes movable pressing blocks 1, elastic bases 2, and supports 4, which are arranged correspondingly at the top and bottom; the movable pressing blocks 1 and elastic bases 2 are both arranged on the same side of the supports.

[0028] In this embodiment, the movable pressure block 1 includes an adhesive layer 11 and a first movable base 12. The bottom of the first movable base 12 is provided with an adhesive layer 11 for tearing the skin on the back of the tobacco leaf 3. The first movable base 12 is installed on a vertical track 13. The first movable base 12 is manually driven to move along the vertical track 13. The position of the manual hand can be selected as the top or side of the first movable base 12 as the hand pressure surface.

[0029] In this embodiment, the adhesive surface 11 is double-sided adhesive and needs to be replaced after each use.

[0030] Since it is difficult to control the force of manual hand-pressing, a flexible pressure head, such as a relatively soft block structure like sponge or silicone, can be added between the adhesive surface 11 and the first movable base 12 to solve the problem of unstable downward pressure and avoid damaging the tobacco leaf 3.

[0031] In other embodiments, the first movable base 12 can also be replaced by an automatic drive, such as a linear module drive, a cylinder drive, an electric actuator drive, an electric lead screw drive, etc.

[0032] The upper surface of the elastic base 2 is provided with a fixing component 21 for fixing the front side of the tobacco leaf 3. In this embodiment, the fixing component 21 is a double-sided adhesive, the lower surface of which is glued to the second movable base 22, and the upper surface is used to glue and fix the tobacco leaf 3. The fixing component 21 is also designed to be replaced after use.

[0033] In terms of adhesive design, the adhesiveness of the fixing component 21 is greater than or equal to the adhesiveness of the adhesive layer 11.

[0034] The main reason for this design is that during the growth process of tobacco leaves, the front side is mainly exposed to sunlight and the external environment, and its texture is relatively harder and stronger than the back side, making it less likely to separate. The back side is relatively more fragile than the front side and is easier to tear apart. This solution utilizes this difference in characteristics so that when both sides are subjected to tearing force during the pressing process, the back side is easier to tear off than the front side, ensuring that the exposed surface of the protoplasm is always the back side, which also provides the basis for the implementation of this device.

[0035] In terms of specific structural design, the elastic base 2 includes a second movable base 22 and a spring 23. The second movable base 22 is slidably mounted in the vertical track 13. A fixing component 21 is provided on the upper surface of the second movable base 22. The structure of the second movable base 22 is the same as that of the first movable base 12, but the installation direction is opposite. In addition, a flexible pressure head 14 is also installed between the second movable base 22 and the fixing component 21 to buffer the pressure and solve the problem of unstable pressure.

[0036] A connecting rod is connected to the side of the second movable base 22. The connecting rod is horizontally positioned, and a spring 23 is fixed above the connecting rod. The spring 23 is vertically positioned, with its upper end fixed to the bracket 4 and its lower end connected to the connecting rod. Therefore, during the reciprocating motion of the second movable base 22, the spring 23 provides a restoring force for the second movable base 22.

[0037] The elastic design of the elastic base 2 is also to overcome the problem of uncontrollable downward pressure during manual operation. Through the adaptive adjustment of the elastic base 2, the downward pressure is relatively more stable.

[0038] In terms of the design of the effective area, the pressing surface of the movable pressing block 1 is less than or equal to the supporting surface of the elastic base 2, and the tearing area is controllable, which can extract a sufficient amount of protoplasts.

[0039] Working principle explanation: This utility model is a manual device. The adhesive layer 11 on the lower end face of the movable pressure block 1 is an adhesive layer that can be torn off and replaced at any time. When preparing, the double-sided tape can be cut into a fixed size and kept for later use.

[0040] First, stick the front side of tobacco leaf 3 onto the double-sided tape (i.e., fixing component 21), peel off the other side of the double-sided tape, and attach and fix the double-sided tape to the elastic base. The second step is to take another double-sided tape (i.e., tape side 11), peel off one side of the double-sided tape and attach it to the bottom of the first movable base 12, with the other side of the double-sided tape facing down and the outer skin peeled off, to be used as the tearing surface.

[0041] Third step, hold the movable pressing block 1 and press it down tightly against the tobacco leaf 3. The double-sided tape on top adheres to the surface of the tobacco leaf 3. Then lift the movable pressing block 1. Driven by the separation force, the surface of the tobacco leaf 3 is torn off, exposing the protoplast inside.

[0042] Fourth, remove the top and bottom double-sided tape and any attached materials. Keep the tobacco leaf 3 and the double-sided tape together for later use. Dispose of the torn-off part and the double-sided tape together as waste.

[0043] This completes the processing of one tobacco leaf (3). Follow the steps above to process other tobacco leaves.

[0044] The above specific embodiments are only used to illustrate the technical solution of this utility model and are not intended to limit it. Although this utility model has been described in detail with reference to examples, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solution of this utility model without departing from the scope of the technical solution of this utility model, and all such modifications or substitutions should be covered within the protection scope of this utility model.

Claims

1. A tobacco leaf epidermis tearing device, characterized by, Includes movable pressure blocks and elastic bases that are set up correspondingly at the top and bottom; The movable pressing block includes an adhesive layer and a first movable base. The adhesive layer is disposed on the lower surface of the first movable base and is used to tear the epidermis on the back of the tobacco leaf. The upper surface of the elastic base is provided with a fixing component, which is used to fix the front side of the tobacco leaf.

2. The tobacco leaf epidermal tear device of claim 1, wherein, The adhesive layer is double-sided adhesive.

3. The tobacco leaf epidermal tear device of claim 1, wherein, The tobacco leaf peeling device also includes a vertically arranged sliding rail, on which the first movable base is slidably disposed.

4. The tobacco leaf epidermal tear device of claim 3, wherein, The first movable base is driven by one of the following methods: manual drive, linear module drive, cylinder drive, electric actuator drive, or electric lead screw drive.

5. The tobacco leaf epidermal tear device of claim 3, wherein, The tobacco leaf peeling device also includes a support frame, and the sliding track is disposed on the side of the support frame.

6. The tobacco leaf epidermal tear device of claim 1, wherein, A flexible pressure head is provided between the first movable base and the adhesive layer.

7. The tobacco leaf epidermal tear device of claim 6, wherein, The flexible pressure head is made of sponge or silicone.

8. The tobacco leaf epidermal tear device of claim 2, wherein, The fixing component is double-sided adhesive.

9. The tobacco leaf peeling device according to claim 8, characterized in that, The adhesiveness of the fixing component is greater than that of the adhesive layer.

10. The tobacco leaf peeling device according to claim 1, characterized in that, The upper surface area of ​​the fixing component is greater than the lower surface area of ​​the adhesive layer.

11. The tobacco leaf peeling device according to claim 5, characterized in that, The elastic base includes a second movable base and an elastic component. The second movable base is slidably disposed on the sliding track. One side of the second movable base is connected to the elastic component, and the other end of the elastic component is fixed to the bracket.

12. The tobacco leaf peeling device according to claim 11, characterized in that, The elastic component is a spring.

13. The tobacco leaf peeling device according to claim 12, characterized in that, A connecting rod is provided on one side of the second movable base, and a spring is provided above the connecting rod. The upper end of the spring is fixed to the bracket, and the lower end of the spring is connected to the connecting rod.

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