An ultra-low temperature biological sample pulverizing device

Abstract

This utility model relates to the field of pulverizing equipment technology, and in particular to a cryogenic biological sample pulverizing device. The cryogenic biological sample pulverizing device includes: a pulverizer body, a lifting plate, positioning blocks, a positioning component, a pusher plate, and a drive component. The pulverizer body has a placement platform for placing a material container and a crushing cylinder installed inside. The lifting plate is installed inside the placement platform, and positioning blocks are equidistantly installed inside the placement platform. A positioning component is installed between the placement platform and the positioning blocks. The cryogenic biological sample pulverizing device provided by this utility model achieves precise docking of the material container through the design of the positioning component. Through the coordinated action of multiple equidistantly distributed positioning blocks, the material container is precisely pushed to the center of the lifting plate, ensuring that the center of the material container completely coincides with the center of the subsequent crushing cylinder inlet. This greatly shortens the feeding and positioning time, improves operating efficiency, and effectively avoids sample loss caused by inaccurate positioning.

Description

Technical Field

[0001] This utility model relates to the field of pulverizing equipment technology, and in particular to an ultra-low temperature biological sample pulverizing device. Background Technology

[0002] In fields such as biomedical research, agricultural science, food engineering, and environmental monitoring, ultra-low temperature biological sample pulverization technology is a core supporting means to achieve efficient sample processing and preservation of active ingredients. This technology uses cooling media such as liquid nitrogen to cool biological samples to their brittle point, causing the hydrogen bonds inside the sample to break and the tissue structure to loosen. Subsequently, through mechanical crushing, it decomposes the sample into micron-sized or even nano-sized particles. This avoids protein denaturation, nucleic acid degradation, and loss of volatile components caused by mechanical friction heat generated during room temperature pulverization, while maximizing the release of target analytes inside the sample. This provides high-quality sample pretreatment for subsequent experiments such as gene sequencing, proteomics analysis, and active substance extraction. However, existing cryogenic biological sample crushing equipment has design flaws in its feeding process. Currently, most equipment requires workers to manually place the sample-filled bucket onto the internal lifting platform during the feeding process. The position of the bucket is then visually adjusted to align with the feed inlet on the outer wall of the crushing cylinder. Subsequently, locking cylinders on both sides of the feed inlet are activated to fix the bucket to the feed inlet. This manual positioning method has many problems: First, the positioning accuracy is extremely low. Due to the lack of standardized positioning benchmarks, workers cannot guarantee that the center of the bucket and the center of the feed inlet are completely aligned by visual judgment alone. This results in a loose connection between the bucket and the feed inlet and may also cause some samples to scatter during the feeding process, resulting in sample loss. Second, the operation efficiency is low. For batch sample processing scenarios, workers need to adjust the positioning of each bucket one by one, which significantly prolongs the sample pretreatment cycle and reduces work efficiency.

[0003] Therefore, it is necessary to provide a new cryogenic biological sample pulverization device to solve the above-mentioned technical problems. Utility Model Content

[0004] To solve the above-mentioned technical problems, this utility model provides an ultra-low temperature biological sample pulverizing device.

[0005] The ultra-low temperature biological sample pulverizing equipment provided by this utility model includes: a pulverizer body, a lifting plate, positioning blocks, a positioning component, a push plate, and a drive component. The pulverizer body is equipped with a placement platform for placing a material bucket and a crushing cylinder. The placement platform is equipped with a lifting plate. Positioning blocks are equidistantly installed inside the placement platform. A positioning component is installed between the placement platform and the positioning blocks. The positioning component drives the positioning blocks to converge and position the material bucket at the center of the lifting plate. A push plate is provided at the top of one end of the placement platform near the outer side. A drive component is installed between the placement platform and the push plate. The drive component drives the push plate to move and push the material bucket placed on the placement platform toward the lifting plate.

[0006] Preferably, the positioning components include: a motor, a gear, a gear ring, and a turntable. An electric push rod is fixedly connected inside the crusher body, and its output shaft end is fixedly connected to the axis of the lifting plate. The turntable is sleeved on the outer wall of the output shaft of the electric push rod, and the turntable is rotatably connected to the bottom of the placement platform. A gear ring is fixedly sleeved on the outer side of the turntable. A motor output end is fixedly connected inside the crusher body, and the gear meshes with the gear ring. Arc-shaped grooves are equidistantly opened on the inner circumference of the turntable. A protruding rod is fixedly connected to the bottom of each positioning block, and the protruding rod is placed inside the corresponding arc-shaped groove.

[0007] Preferably, the placement platform has equidistant grooves inside, and the positioning blocks are all placed inside the corresponding grooves. The output shaft of the electric push rod is fitted with a guide block in the middle. The guide block is cross-shaped, and its ends are all designed with bevels. The bottom end of the protruding rod is in contact with the corresponding bevel. The bottom end of the guide block is fixedly connected to the inner wall of the crusher body through a vertical rod.

[0008] Preferably, the drive assembly includes: a cylinder and a connecting rod 1. Both ends of the push plate are fixedly connected to the connecting rod 1. Cylinders are symmetrically fixedly connected inside the crusher body, and their output ends are fixedly connected to the other end of the corresponding connecting rod 1.

[0009] Preferably, a pull plate is slidably connected to the top of the placement platform away from the push plate, and a connecting rod 2 is fixedly connected to the bottom of the pull plate. A locking block is rotatably connected to the other end of the connecting rod 2, and a torsion spring is installed at the rotatable connection between the locking block and the connecting rod 2. The end of the locking block away from the connecting rod 2 is designed with a bevel. A tension spring is fixedly connected to the outer wall of the end of the connecting rod 2 near the pull plate, and the other end of the tension spring is fixedly connected to the inner wall of the crusher body.

[0010] Preferably, the outer wall of the connecting rod near the cylinder output end is fixedly connected with a locking rod that matches the inclined surface of the locking block, and the bottom of the placement platform is symmetrically fixedly connected with a top rod corresponding to the locking block.

[0011] Preferably, the inside of the lifting plate is provided with a clearance groove corresponding to the positioning block.

[0012] Compared with related technologies, the cryogenic biological sample pulverizing equipment provided by this utility model has the following beneficial effects: The design of the positioning components enables precise docking of the material bucket. Through the coordinated action of multiple equidistant positioning blocks, the material bucket is precisely pushed to the center of the lifting plate, ensuring that the center of the material bucket is completely aligned with the center of the feed inlet of the subsequent crushing cylinder. This greatly shortens the feeding and positioning time, improves operating efficiency, and effectively avoids sample loss caused by inaccurate positioning.

[0013] Through the design of the drive components, the staff only needs to place the material bucket in the feeding area between the push plate and the pull plate. The cylinder drives the push plate to push the material bucket to the working area. At the same time, after the sample is crushed, the pull plate can be driven to reset the material bucket, reducing manual intervention and greatly improving work efficiency. Attached Figure Description

[0014] Figure 1 A schematic diagram of the structure of the ultra-low temperature biological sample pulverizing device provided by this utility model; Figure 2 for Figure 1 The diagram shows the internal structure of the pulverizer body. Figure 3 for Figure 2 The diagram shows a cross-sectional view of the placement platform. Figure 4 for Figure 3 The diagram shows the structure of the turntable. Figure 5 for Figure 4 The diagram shows the structure of the card block; Figure 6 for Figure 4 The diagram shows the structure at point A.

[0015] The following are the labels in the diagram: 1. Crusher body; 2. Placement platform; 3. Crushing cylinder; 4. Lifting plate; 5. Positioning block; 6. Push plate; 7. Motor; 8. Gear; 9. Gear ring; 10. Turntable; 11. Electric push rod; 12. Arc groove; 13. Guide block; 14. Cylinder; 15. Connecting rod one; 16. Pull plate; 17. Connecting rod two; 18. Locking block; 19. Tension spring; 20. Locking rod; 21. Top rod. Detailed Implementation

[0016] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely for explaining the present utility model and are not intended to limit the present utility model.

[0017] The specific implementation of this utility model will be described in detail below with reference to specific embodiments.

[0018] Please see Figures 1 to 6A cryogenic biological sample pulverizing device is disclosed, comprising: a pulverizer body 1, a lifting plate 4, positioning blocks 5, a positioning component, a pusher plate 6, and a drive component. The pulverizer body 1 is internally equipped with a material container placement platform 2 and a crushing cylinder 3. The lifting plate 4 is internally equipped with the placement platform 2. Positioning blocks 5 are equidistantly installed internally with the placement platform 2. A positioning component is installed between the placement platform 2 and the positioning blocks 5. The positioning component drives the positioning blocks 5 to converge, positioning the material container at the center of the lifting plate 4. A pusher plate 6 is provided at the top of one end of the placement platform 2 near the outer side. A drive component is installed between the placement platform 2 and the pusher plate 6. The drive component drives the pusher plate 6 to move, pushing the material container placed on the placement platform 2 toward the lifting plate 4.

[0019] The positioning components include: a motor 7, a gear 8, a gear ring 9, and a turntable 10. An electric push rod 11 is fixedly connected inside the crusher body 1, and its output shaft end is fixedly connected to the axis of the lifting plate 4. The turntable 10 is sleeved on the outer wall of the output shaft of the electric push rod 11, and the turntable 10 is rotatably connected to the bottom of the placement platform 2. A gear ring 9 is fixedly sleeved on the outer side of the turntable 10. A motor 7 is fixedly connected inside the crusher body 1, and a gear 8 is fixedly connected to the output end of the motor 7, and the gear 8 meshes with the gear ring 9. Arc-shaped grooves 12 are equidistantly opened around the inner circumference of the turntable 10. A protruding rod is fixedly connected to the bottom of each positioning block 5, and the protruding rod is placed inside the corresponding arc-shaped groove 12.

[0020] The placement platform 2 has equidistant grooves inside, and the positioning blocks 5 are all placed inside the corresponding grooves. The output shaft of the electric push rod 11 is fitted with a guide block 13. The guide block 13 is cross-shaped, and its ends are all designed with bevels. The bottom end of the protruding rod is in contact with the corresponding bevel. The bottom end of the guide block 13 is fixedly connected to the inner wall of the crusher body 1 through a vertical rod.

[0021] The drive assembly includes a cylinder 14 and a connecting rod 15. Both ends of the push plate 6 are fixedly connected to the connecting rod 15. The cylinder 14 is symmetrically fixedly connected inside the crusher body 1, and its output end is fixedly connected to the other end of the corresponding connecting rod 15.

[0022] A pull plate 16 is slidably connected to the top of the placement platform 2 away from the push plate 6. A connecting rod 17 is fixedly connected to the bottom of the pull plate 16. A locking block 18 is rotatably connected to the other end of the connecting rod 17. A torsion spring is installed at the rotatable connection between the locking block 18 and the connecting rod 17. The end of the locking block 18 away from the connecting rod 17 is designed with a bevel. A tension spring 19 is fixedly connected to the outer wall of the end of the connecting rod 17 near the pull plate 16. The other end of the tension spring 19 is fixedly connected to the inner wall of the crusher body 1.

[0023] The outer wall of the connecting rod 15 near the output end of the cylinder 14 is fixedly connected with a locking rod 20 that matches the inclined surface of the locking block 18, and the bottom of the placement platform 2 is symmetrically fixedly connected with a top rod 21 corresponding to the locking block 18.

[0024] The inside of the lifting plate 4 is provided with a clearance groove corresponding to the positioning block 5.

[0025] It should be noted that: the inclined end of the guide block 13 is magnetically designed, so that after the convex rod is reset, it can drive the positioning block 5 to be stored in the slide groove of the placement platform 2; the top rod 21 is fixed to the bottom of the placement platform 2 and does not interfere with the movement of the clamping rod 20; a control cabinet is installed on one side of the crusher body 1 for controlling the start-up of the crusher body 1 and setting parameters, etc.; the positioning block 5 has an adapter groove inside, and a square rod is slidably installed inside the adapter groove, so that the positioning rod 5 can move smoothly horizontally and vertically; In the initial state, the feed inlet installed on the side wall of the crushing cylinder 3 faces downward, and locking cylinders are installed on both sides of the feed inlet. After the material barrel is fixed, the drive mechanism inside the crusher body 1 drives the crushing cylinder 3 to rotate 180 degrees. Then, the crushing operation begins through the crushing components inside. After the crushing is completed, it rotates 180 degrees in the opposite direction so that the feed inlet faces downward.

[0026] The working principle of the ultra-low temperature biological sample pulverizing device provided by this utility model is as follows: The staff placed the bucket containing the ultra-low temperature biological sample to be crushed in the loading area of ​​the placement platform 2, that is, the open area between the push plate 6 and the pull plate 16, without deliberately aligning the center, to complete the loading preparation. Next, cylinder 14 is activated, and its output end begins to extend. Since both ends of push plate 6 are fixedly connected to the output end of cylinder 14 via connecting rod 15, the thrust of cylinder 14 is synchronously transmitted to push plate 6 via connecting rod 15. Driven by cylinder 14, push plate 6 moves smoothly along the top of placement platform 2 towards lifting plate 4, pushing the material bucket placed on placement platform 2 to move synchronously in the lifting plate 4 area. During this process, the locking rod 20 fixed to the outer wall of connecting rod 15 near the output end of cylinder 14 will gradually engage with the bottom of pull plate 16. The inclined surface of the locking block 18 on the second end connecting rod 17 is in contact; as the first connecting rod 15 continues to move, the locking rod 20 exerts a squeezing force on the inclined surface of the locking block 18, forcing the locking block 18 to rotate downward around its rotational connection with the second connecting rod 17, and simultaneously compressing the torsion spring at the connection; when the locking rod 20 completely passes the end of the inclined surface of the locking block 18, the torsion spring releases its elastic force instantly, pushing the locking block 18 back to its initial horizontal state; the push plate 6 pushes the material bucket to the area directly above the lifting plate 4 and then stops moving, completing the initial positioning; After the material bucket is pushed into place, the motor 7 in the positioning component starts, and its output end drives the fixedly connected gear 8 to rotate. Since the gear 8 meshes with the gear ring 9 fixedly sleeved on the outside of the turntable 10, the rotation driving force of the gear 8 is transmitted to the gear ring 9, which in turn drives the turntable 10 inside the gear ring 9 to rotate around the output shaft of the electric push rod 11. The turntable 10 has equidistant arc-shaped grooves 12 around its circumference, and each positioning block 5 has a protruding rod fixedly connected to its bottom, with each protruding rod placed inside its corresponding arc-shaped groove 12. When the turntable 10 rotates, the groove wall of the arc-shaped groove 12 exerts a circumferential squeezing force on the protruding rod, forcing the protruding rod to move the positioning block 5 towards the center of the placement platform 2 along the sliding groove inside the placement platform 2. At the same time, since the output shaft of the electric push rod 11 is fitted with a cross-shaped guide block 13, the end of the guide block 13 is designed with a slope and is fixed to the inner wall of the crusher body 1 by a vertical rod. The bottom end of the protruding rod at the bottom of the positioning block 5 is in contact with the corresponding slope of the guide block 13. As the protruding rod moves towards the center, its bottom end will gradually slide upward along the slope of the guide block 13, thereby driving the positioning block 5 to move towards the center while simultaneously lifting upward. Under the combined motion of "convergence + lifting", the inner wall of the positioning block 5 gradually contacts the outer wall of the material bucket and generates a uniform clamping force. Through the coordinated action of multiple equidistantly distributed positioning blocks 5, the material bucket is pushed to the center of the lifting plate 4, ensuring that the center of the material bucket is completely coincident with the center of the feed inlet of the subsequent crushing cylinder 3, and the positioning component completes its work. After the material bucket is centered, the motor 7 reverses and drives the turntable 10 to rotate in the opposite direction through the meshing of the gear 8 and the gear ring 9. The arc groove 12 on the turntable 10 rotates in the opposite direction, and the force exerted by the groove wall on the bottom protrusion of the positioning block 5 changes, pushing the protrusion to move the positioning block 5 away from the center along the slide. At the same time, the bottom end of the protrusion gradually slides down the inclined surface of the guide block 13, and the positioning block 5 descends accordingly, and is finally completely stored back in the slide of the placement platform 2. Then the electric push rod 11 starts, and its output shaft drives the lifting plate 4 to extend upward. The lifting plate 4 drives the top material bucket to lift upward synchronously. When the feed end of the material bucket is completely aligned and in contact with the feed port installed on the outside of the crushing cylinder 3, the electric push rod 11 stops extending. Then, the locking cylinders preset on both sides of the feed port of the crushing cylinder 3 start automatically, and their output ends extend and tightly fix the material bucket to the feed port, ensuring that the sample will not scatter during the ultra-low temperature crushing process, while ensuring the sealing of the crushing chamber. Staff members set core parameters such as crushing speed and crushing time according to the type of biological sample, embrittlement temperature, and particle size requirements through the equipment control panel. Then, the crusher body 1 is started, and the crushing components in the crushing cylinder 3 begin to work according to the set parameters to mechanically crush the biological sample in the material barrel that is already in an ultra-low temperature embrittlement state. Because the entire process is carried out in an ultra-low temperature environment, it effectively avoids protein denaturation, nucleic acid degradation, and loss of volatile components caused by mechanical friction heat, ensuring the activity and integrity of the crushed sample. After the sample is crushed, the crushing mechanism automatically stops working. The crushing cylinder 3 rotates to the initial position under the drive mechanism to ensure that the docking state between its feed inlet and the material barrel is stable. Then, the electric push rod 11 extends, so that the lifting plate 4 fits against the bottom of the material barrel. Subsequently, the locking cylinders on both sides of the feed inlet retract, releasing the fixing of the material barrel. The electric push rod 11 resets, and its output shaft retracts downward, driving the lifting plate 4 and the material barrel to descend synchronously until the lifting plate 4 returns to the initial lowest position inside the placement platform 2, and the material barrel is placed back in the working area of ​​the placement platform 2. After the lifting plate 4 is reset, the cylinder 14 starts to retract, and its output end drives the connecting rod 15 and the push plate 6 to move away from the initial direction of the lifting plate 4. Since the locking block 18 and the locking rod 20 are engaged, the connecting rod 15 drives the locking block 18 to move synchronously through the locking rod 20. The locking block 18 pulls the pulling plate 16 to move towards the push plate 6 through the connecting rod 27. At the same time, the tension spring 19 on the outer wall of the connecting rod 27 is stretched, which smoothly pushes the material bucket on the lifting plate 4 to the initial feeding position of the placement platform 2, completing the reset of the material bucket. When the pull plate 16 moves the material bucket back to the feeding position, the inclined surface of the locking block 18 on the connecting rod 2 17 gradually contacts the top rod 21 symmetrically fixed at the bottom of the placement platform 2. As the cylinder 14 continues to contract, the top rod 21 exerts a squeezing force on the inclined surface of the locking block 18, forcing the locking block 18 to rotate downwards again around its rotating connection with the connecting rod 2 17, disengaging from the locking state with the locking rod 20. After the locking is released, the tension spring 19 releases its elasticity, pulling the connecting rod 2 17 and the pull plate 16 back to their initial positions. At this point, the entire equipment returns to its initial preparation state before feeding. Then, the staff directly removes the material bucket containing the crushed sample from the feeding position of the placement platform 2, completing the entire process of single-time cryogenic biological sample crushing.

[0027] The above description is merely an embodiment of this utility model and does not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the content of this utility model specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.

Claims

1. A cryogenic biological sample pulverizing device, characterized in that, include: The crusher body (1) has a material storage platform (2) and a crushing cylinder (3) installed inside the crusher body (1). Lifting plate (4), the lifting plate (4) is installed inside the placement platform (2); Positioning blocks (5) are installed at equal intervals inside the placement platform (2); A positioning component is installed between the placement platform (2) and the positioning block (5). The positioning component drives the positioning block (5) to converge and position the material bucket at the center of the lifting plate (4). Push plate (6), the top of the placement table (2) near the outer side is provided with push plate (6); A drive assembly is installed between the placement platform (2) and the push plate (6). The drive assembly drives the push plate (6) to move, pushing the bucket placed on the placement platform (2) toward the lifting plate (4).

2. The cryogenic biological sample pulverizing device according to claim 1, characterized in that, The positioning components include: a motor (7), a gear (8), a gear ring (9) and a turntable (10). An electric push rod (11) is fixedly connected inside the crusher body (1), and its output shaft end is fixedly connected to the center of the lifting plate (4). The turntable (10) is sleeved on the outer wall of the output shaft of the electric push rod (11), and the turntable (10) is rotatably connected to the bottom of the placement platform (2). A gear ring (9) is fixedly sleeved on the outer side of the turntable (10). A motor (7) is fixedly connected inside the crusher body (1), and a gear (8) is fixedly connected to the output end of the motor (7), and the gear (8) meshes with the gear ring (9). Arc grooves (12) are equidistantly opened around the inner circumference of the turntable (10). A protruding rod is fixedly connected to the bottom of each positioning block (5), and the protruding rod is placed inside the corresponding arc groove (12).

3. The cryogenic biological sample pulverizing device according to claim 2, characterized in that, The placement platform (2) has equidistant grooves inside, and the positioning blocks (5) are all placed inside the corresponding grooves. The output shaft of the electric push rod (11) is fitted with a guide block (13). The guide block (13) is cross-shaped, and its ends are all designed with bevels. The bottom end of the protruding rod is in contact with the corresponding bevel. The bottom end of the guide block (13) is fixedly connected to the inner wall of the crusher body (1) through the vertical rod.

4. The cryogenic biological sample pulverizing device according to claim 1, characterized in that, The drive assembly includes a cylinder (14) and a connecting rod (15). Both ends of the push plate (6) are fixedly connected to the connecting rod (15). The inside of the crusher body (1) is symmetrically fixedly connected to the cylinder (14), and its output end is fixedly connected to the other end of the corresponding connecting rod (15).

5. The cryogenic biological sample pulverizing device according to claim 4, characterized in that, The top of the placement platform (2) is slidably connected to the end away from the push plate (6) with a pull plate (16). The bottom of the pull plate (16) is fixedly connected to the second connecting rod (17). The other end of the second connecting rod (17) is rotatably connected to a locking block (18). A torsion spring is installed at the rotatable connection between the locking block (18) and the second connecting rod (17). The end of the locking block (18) away from the second connecting rod (17) is designed with a slope. The outer wall of the end of the second connecting rod (17) close to the pull plate (16) is fixedly connected to a tension spring (19). The other end of the tension spring (19) is fixedly connected to the inner wall of the crusher body (1).

6. The cryogenic biological sample pulverizing device according to claim 5, characterized in that, The outer wall of the connecting rod (15) near the output end of the cylinder (14) is fixedly connected with a locking rod (20) that matches the inclined surface of the locking block (18), and the bottom of the placement platform (2) is symmetrically fixedly connected with a top rod (21) corresponding to the locking block (18).

7. The cryogenic biological sample pulverizing device according to claim 1, characterized in that, The inside of the lifting plate (4) is provided with a clearance groove corresponding to the positioning block (5).

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