A test tube rack for single-tube cell culture

Abstract

This invention provides a test tube holder for single-tube cell culture, belonging to the field of biological experimental equipment technology. It includes a support base, a bracket assembly, and an adjustment mechanism. The bracket assembly comprises a waist bracket, a top bracket, and a connecting rod, with the connecting rod fixedly connected between the waist bracket and the top bracket. The waist bracket has several placement holes for supporting culture tubes. The adjustment mechanism connects the support base and the bracket assembly, including a coarse adjustment component and a fine adjustment component. The coarse adjustment component is rotatably connected to the support base and can drive the entire bracket assembly to flip and lock, achieving a wide range of tilt angle adjustment. The fine adjustment component is disposed on the coarse adjustment component and is drivenly connected to the waist bracket, driving the waist bracket to slightly deflect relative to the coarse adjustment component and lock, achieving precise calibration of the tilt angle. This invention combines coarse and fine adjustment of the culture tube tilt angle, adapting to the differentiated tilt angle requirements of each step in single-tube cell culture, ensuring cell viability and the reproducibility of experimental data.

Description

Technical Field

[0001] This utility model relates to the field of biological experimental equipment technology, and in particular to a test tube holder for single-tube cell culture. Background Technology

[0002] One-tube cell culture is a core method for fundamental experiments in biomedicine, immunology, and molecular biology. With advantages such as no sample transfer required throughout the process, low risk of contamination, and simplified operation, it is widely used in cell proliferation, cell differentiation, drug cytotoxicity testing, and primary cell isolation. In the complete one-tube cell culture process, key steps such as cell settling, stratified incubation with culture medium, medium exchange and gentle settling, and staining in the dark all require maintaining the culture tube containing the cell suspension at a specific tilt angle to regulate the cell settling rate and optimize the contact area between the cells and the culture medium. A typical tube holder generally includes a base and a bracket fixed to the base. The bracket has several holes for inserting culture tubes; simply inserting the culture tube into the hole completes the placement.

[0003] However, conventional test tube racks have a fixed connection between the holder and the base, and the rack's tilt angle is not adjustable. It can only maintain a horizontal or a single fixed tilt, failing to simultaneously accommodate the varying tilt angle requirements of different processes such as static incubation, staining, etc. When a specific tilt angle is required for a particular process and the test tube rack is unavailable, operators must manually support the tubes to maintain the angle. This not only makes it difficult to ensure consistent angles but also increases the risk of contamination, ultimately affecting cell viability and the reproducibility of experimental data.

[0004] Therefore, it is necessary to propose a test tube holder for single-tube cell culture to solve the above problems. Utility Model Content

[0005] This application provides a test tube holder for single-tube cell culture. In order to improve the technical problem that the tilt angle of the holder in the related technology is not adjustable, and can only maintain a horizontal or single fixed tilt state, it is impossible to simultaneously take into account the different tilt angle requirements of culture test tubes in various processes such as static placement, incubation, and staining.

[0006] This application provides a test tube holder for single-tube cell culture, including a support base, a bracket assembly, and an adjustment mechanism. The bracket assembly includes a waist bracket, a top bracket, and a connecting rod, with the connecting rod fixedly connected between the waist bracket and the top bracket. The waist bracket has several placement holes for supporting culture test tubes. The adjustment mechanism connects the support base and the bracket assembly, including a coarse adjustment component and a fine adjustment component. The coarse adjustment component is rotatably hinged to the support base, which can drive the entire bracket assembly to flip and lock in position, achieving a wide range of tilt angle adjustment. The fine adjustment component is disposed on the coarse adjustment component and is drivenly connected to the waist bracket, which can drive the waist bracket to deflect slightly relative to the coarse adjustment component and lock in position, achieving precise calibration of the tilt angle.

[0007] The technical solutions described above in this application embodiment have at least the following technical effects: By coarse adjustment component and fine adjustment component working together, the tilt angle of the culture tube can be adjusted and locked in a wide range by the coarse adjustment component and then slightly deflected and precisely locked by the fine adjustment component. This solves the problem that the tilt angle of the tube holder is not adjustable and it is difficult to match the different tilt angle requirements of each step of the single-tube cell culture process in the prior art. It enables the culture tube to maintain a precise and stable tilt angle in the steps of static settling, layered incubation, medium change and static settling, staining and light protection, etc., ensuring cell viability and the repeatability of experimental data.

[0008] In some embodiments, the coarse adjustment assembly includes a flipping frame, a guide bracket, a support rod, a pin, and a rotating rod. The flipping frame is fitted onto the outside of the waist support, the guide bracket is fixed to the back of the support base, the support rod is located on the side of the flipping frame, and the bottom end of the support rod is rotatably connected to the support base via a pin. Multiple limiting arc-shaped right-angle teeth are arranged on the inner side of the guide bracket. The rotating rod can rotatably pass through the inside of the flipping frame and its two ends extend out to both sides of the frame. The two ends of the rotating rod are correspondingly provided with limiting arc-shaped right-angle teeth. The limiting arc-shaped right-angle teeth and the limiting arc-shaped right-angle teeth engage with each other to lock the flipping angle.

[0009] In some embodiments, the coarse adjustment assembly further includes a handle and a paddle; the handle is fixed to the back of the flip frame, the paddle is located inside the handle and its end is fixedly connected to the outer surface of the rotating rod; the inner cavity of the limiting arc-shaped right-angle tooth 2 is provided with a cavity, the end of the rotating rod 1 extends into the cavity, a torsion spring is provided in the cavity, and the two ends of the torsion spring are fixedly connected to the inner wall of the cavity and the end of the rotating rod 1 respectively, for driving the limiting arc-shaped right-angle tooth 2 to reset and engage.

[0010] In some embodiments, the fine-tuning assembly includes a drive rod, a second rotating rod, a locking disc, a damping spring assembly, and a limiting disc; the second rotating rod is fixedly connected to the waist support, the drive rod and the second rotating rod are coaxially arranged, and the two are slidably engaged and can rotate synchronously; the locking disc is fixedly mounted on the drive rod, the damping spring assembly is located between the locking disc and the limiting disc, the two ends of the damping spring assembly are fixedly connected to the locking disc and the limiting disc respectively, and the limiting disc is rotatably connected to the support rod.

[0011] In some embodiments, the fine-tuning assembly further includes an indicator sleeve and a locking block; the indicator sleeve is sleeved on the outside of the drive rod, the outer side of the indicator sleeve is provided with angle markings, and multiple locking holes are provided; a locking block is fixed on the locking disc, and the locking block can be inserted into the locking hole to achieve angle positioning; the outer surface of the rotating rod is provided with a limiting protrusion along the axial direction, and the inner wall of the drive rod is provided with a matching limiting groove, and the limiting protrusion is slidably embedded in the limiting groove to achieve circumferential limiting.

[0012] In some embodiments, the inner wall of each placement hole is provided with an adaptive expansion ring, which is used to adaptively clamp culture tubes with different outer diameters.

[0013] In some embodiments, the bottom of the support base is provided with multiple anti-slip pads and counterweights, the counterweights being used to lower the overall center of gravity of the frame; the top bracket is provided with limiting holes corresponding to the placement holes, which are used to limit the upper part of the culture tube.

[0014] The beneficial effects of this invention are as follows: By coarse adjustment and fine adjustment components working together, this invention enables the tilt angle of the culture tube to be adjusted and locked in place by the coarse adjustment component, and then slightly deflected and precisely locked by the fine adjustment component. This solves the problems of the inability to adjust the tilt angle of the tube holder in the prior art and the difficulty in matching the different tilt angle requirements of each step in the single-tube cell culture process. It enables the culture tube to maintain a precise and stable tilt angle in the steps of static settling, layered incubation, medium change and static settling, staining and light protection, etc., ensuring cell viability and the reproducibility of experimental data. Attached Figure Description

[0015] Figure 1 A schematic diagram of the overall structure of the test tube rack provided in this embodiment of the utility model; Figure 2 for Figure 1 Another structural diagram of the central support base; Figure 3 for Figure 1 Schematic diagram of the coarse and medium adjustment components; Figure 4 for Figure 3 Enlarged structural diagram at point A; Figure 5 for Figure 3 Another perspective exploded view of the center handle; Figure 6 for Figure 1 Schematic diagram of the mid-waist support; Figure 7 for Figure 6 A magnified structural diagram at point B in the middle.

[0016] The following are the labeling elements in the figure: 111. Support base; 112. Waist support; 113. Top support; 114. Connecting rod; 115. Anti-slip mat; 116. Counterweight; 117. Self-adjusting expansion ring; 2. Adjustment mechanism; 21. Coarse adjustment component; 211. Flipping frame; 212. Handle; 213. Guide bracket; 214. Pin; 215. Support rod; 216. Rotating rod one; 217. Limiting arc-shaped right-angle tooth one; 21 8. Limiting arc-shaped right-angle teeth (2); 219. Cavity; 210. Torsion spring; 2111. Paddle; 22. Fine-tuning assembly; 221. Indicator sleeve; 222. Angle markings; 223. Locking hole; 224. Rotating rod (2); 225. Limiting protrusion; 226. Drive rod; 227. Limiting groove; 228. Locking disc; 229. Locking block; 220. Damping spring assembly; 2211. Limiting disc. Detailed Implementation

[0017] Based on this, in order to improve the technical problem that the tilt angle of the bracket in the related technology is not adjustable and can only maintain a horizontal or single fixed tilt state, and cannot simultaneously meet the different requirements of each process such as static placement, incubation, staining and other processes for the tilt angle of the culture tube, the embodiments of this application provide the following solution.

[0018] Please refer to the following: Figures 1 to 7 This utility model provides a test tube holder for single-tube cell culture, including a support base 111, a bracket assembly and an adjustment mechanism 2; the bracket assembly includes a waist bracket 112, a top bracket 113 and a connecting rod 114, and the waist bracket 112 has multiple placement holes; the adjustment mechanism 2 includes a coarse adjustment component 21 and a fine adjustment component 22.

[0019] The support base 111 serves as the load-bearing base for the test tube rack, supporting the bracket assembly and adjustment mechanism 2. The support base 111 can be a one-piece molded plate structure, with the bracket assembly mounted on the top front and the guide bracket 213 mounted on the back. The support base 111 can be injection molded from medical-grade polypropylene or polycarbonate, or stamped from stainless steel sheet. This design provides clear installation benchmarks for each component of the test tube rack, resulting in a well-organized overall structure and stable load-bearing capacity.

[0020] A waist support 112 is disposed on top of the support base 111, and has multiple placement holes for supporting culture tubes. The number of placement holes can be set according to the number of culture tubes in a single batch. For example, there are six placement holes, evenly arranged in two rows and three columns. The diameter of the placement holes is larger than the outer diameter of the culture tubes to facilitate easy insertion of the culture tubes. A top support 113 is disposed on top of the support base 111 and above the waist support 112. A connecting rod 114 is fixedly connected between the waist support 112 and the top support 113, thereby connecting the waist support 112 and the top support 113 into a whole and maintaining the gap between them. After the culture tubes are inserted into the placement holes, the top support 113 limits the upper part of the culture tubes, thereby forming a stable constraint on the culture tubes at both the top and bottom points and preventing the culture tubes from shaking during tilting.

[0021] In some embodiments, the top support 113 has limiting holes that correspond one-to-one with the placement holes. The upper part of the culture tube passes through the limiting holes, so that the upper part of the culture tube is radially constrained by the limiting holes, which further improves the positioning reliability of the culture tube in the tilted state.

[0022] In some embodiments, each placement hole has an adaptive expansion ring 117 on its inner wall. The adaptive expansion ring 117 can be made of an elastic material such as silicone, and its inner diameter is smaller than the outer diameter of the culture tube. When culture tubes of different outer diameters are inserted into the placement hole, the adaptive expansion ring 117 undergoes elastic deformation and adaptively conforms to the outer wall of the culture tube, generating a clamping force. This design allows the same tube holder to accommodate culture tubes of different sizes, preventing the culture tubes from shaking or tipping over during tilted incubation, thus reducing the risk of contamination.

[0023] In some embodiments, the bottom of the support base 111 is provided with a plurality of anti-slip pads 115 and counterweights 116. Four anti-slip pads 115 may be provided, respectively located at the four corners of the bottom of the support base 111. The anti-slip pads 115 may be made of silicone rubber or thermoplastic elastomer to increase the friction between the test tube rack and the work surface, preventing the test tube rack from slipping during tilt adjustment or culture. The counterweights 116 are located at the bottom of the support base 111, which can lower the overall center of gravity of the test tube rack. When the waist support 112 rotates the culture test tubes upwards to a larger tilt angle, the counterweights 116 can counteract the tendency to tip over due to the increased center of gravity, improving the stability of the test tube rack in a tilted state.

[0024] The coarse adjustment component 21 is rotatably connected to the support base 111, and is used to drive the entire bracket assembly to rotate and lock in position. The coarse adjustment component 21 includes a rotating frame 211, a guide bracket 213, a support rod 215, a pin 214, and a rotating rod 216. The rotating frame 211 is fitted onto the outside of the waist support 112, and the waist support 112 rotates synchronously with the rotating frame 211; the guide bracket 213 is fixed to the back of the support base 111; the support rod 215 is set on the side of the rotating frame 211, and the bottom end of the support rod 215 is rotatably connected to the support base 111 through the pin 214. Specifically, a support rod 215 is set on each of the left and right sides of the rotating frame 211, and the pin 214 passes through the bottom end of the support rod 215. The two ends of the pin 214 are fixedly connected to the top front of the support base 111, so that the rotating frame 211 can drive the waist support 112 to rotate relative to the support base 111 around the axis of the pin 214. This design ensures that the tilt adjustment of the lumbar support 112 has a stable axis of rotation, resulting in a smooth flipping process and providing the benefits of smooth operation and reliable support.

[0025] The guide bracket 213 has multiple limiting arc-shaped right-angle teeth 217 arranged on its inner side. A rotating rod 216 is rotatably inserted inside the tilting frame 211, with both ends extending out from the sides of the frame. Each end of the rotating rod 216 has a corresponding limiting arc-shaped right-angle tooth 218, which is positioned opposite and meshes with the limiting arc-shaped right-angle teeth 217. When the tilting frame 211 rotates the waist support 112 to the target angle, the limiting arc-shaped right-angle teeth 218 mesh with the corresponding limiting arc-shaped right-angle teeth 217, locking the tilting frame 211 at that tilt angle, thus locking the tilting angle and preventing the tilting frame 211 from retracting due to gravity or external force. The spacing of the limiting arc-shaped right-angle teeth 217 can be set according to the required number of tilt angles. For example, each limiting arc-shaped right-angle tooth 217 is arranged at equal intervals along the arc surface.

[0026] In some embodiments, a cavity 219 is provided inside the limiting arc-shaped right-angle tooth 218, and the end of the rotating rod 216 extends into the cavity 219. A torsion spring 210 is provided inside the cavity 219, and the two ends of the torsion spring 210 are fixedly connected to the inner wall of the cavity 219 and the end of the rotating rod 216, respectively. When the flipping frame 211 flips, the limiting arc-shaped right-angle tooth 218 slides along the tooth surface of the limiting arc-shaped right-angle tooth 217. Under the action of the reverse thrust of the tooth surface, the limiting arc-shaped right-angle tooth 218 rotates slightly around the rotating rod 216 and compresses the torsion spring 210. When the flipping is in place, the torsion spring 210 rebounds and drives the limiting arc-shaped right-angle tooth 218 to reset, so that it re-engages with the adjacent limiting arc-shaped right-angle tooth 217. This setting allows the locking of the flipping angle to be completed automatically by the torsion spring 210, without the need for additional locking by the operator, which facilitates quick angle adjustment.

[0027] In some embodiments, the coarse adjustment assembly 21 further includes a handle 212 and a lever 2111. The handle 212 is fixed to the back of the flip frame 211 for easy lifting by the operator; the lever 2111 is disposed inside the handle 212, and the end of the lever 2111 is fixedly connected to the outer surface of the rotating rod 216. When it is necessary to increase or decrease the tilt angle, the operator pulls the handle 212 upward, causing the flip frame 211 and the waist support 112 to flip around the pin 214; after reaching the target angle, the handle 212 is released, and the torsion spring 210 automatically drives the limiting arc-shaped right-angle tooth 218 to reset and engage, completing the coarse adjustment locking. When it is necessary to lower and retract the flip frame 211, push the lever 2111 on the inside of the handle 212 upwards. The lever 2111 drives the rotating rod 216 to rotate, which in turn drives the two end limiting arc right angle teeth 218 to rotate, so that they disengage from the engagement of the limiting arc right angle teeth 217, and the flip frame 211 can be lowered flat.

[0028] The fine adjustment component 22 is mounted on the coarse adjustment component 21 and is connected to the lumbar support 112 for transmission. It is used to drive the lumbar support 112 to deflect slightly relative to the coarse adjustment component 21 and lock it, so as to achieve precise calibration of the tilt angle. The fine adjustment component 22 includes a drive rod 226, a second rotating rod 224, a locking disc 228, a damping spring assembly 220, and a limiting disc 2211. The second rotating rod 224 is fixedly connected to the lumbar support 112. For example, after the second rotating rod 224 passes through the support rod 215 and the flip frame 211, its end is rigidly fixed to one side of the lumbar support 112; the other side of the lumbar support 112 is simultaneously hinged to the flip frame 211 and the support rod 215 through a pin. The drive rod 226 and the second rotating rod 224 are coaxially arranged, and the drive rod 226 and the second rotating rod 224 are slidably engaged and can rotate synchronously. The locking plate 228 is fixedly mounted on the drive rod 226. The damping spring plate assembly 220 is disposed between the locking plate 228 and the limiting plate 2211, and the two ends of the damping spring plate assembly 220 are fixedly connected to the locking plate 228 and the limiting plate 2211 respectively. The limiting plate 2211 is rotatably connected to the support rod 215. When the drive rod 226 is rotated, the drive rod 226 drives the second rotating rod 224 to rotate synchronously, thereby pulling the waist support 112 to deflect slightly around the axis of the second rotating rod 224. After the drive rod 226 is released, the damping spring plate assembly 220 pushes the locking plate 228 to reset and lock by its own elasticity. This configuration results in a short transmission path and accurate transmission, which helps improve fine-tuning precision. At the same time, the damping spring assembly 220 continuously provides elastic support to prevent the waist support 112 from shifting during fine-tuning.

[0029] In some embodiments, the fine-tuning assembly 22 further includes an indicator sleeve 221 and a locking block 229. The indicator sleeve 221 is sleeved on the outside of the drive rod 226, and the outer side of the indicator sleeve 221 is provided with angle markings 222 and multiple locking holes 223. The locking block 229 is fixed on the locking disc 228, and the locking block 229 can be inserted into the locking hole 223 to achieve angle positioning. During fine-tuning, the drive rod 226 is pressed towards the flip frame 211 to disengage the locking block 229 from the locking hole 223 and release the locking constraint; the drive rod 226 is rotated, and the fine-tuning value is read through the angle markings 222; after adjustment, the drive rod 226 is released, and the locking block 229 is reinserted into the corresponding locking hole 223 to complete angle locking. This configuration makes the fine-tuning angle readable and repeatable, improving the accuracy and consistency of tilt angle calibration.

[0030] In some embodiments, the outer surface of the second rotating rod 224 is provided with a limiting protrusion 225 along the axial direction, and the inner wall of the driving rod 226 is provided with a matching limiting groove 227. The limiting protrusion 225 is slidably embedded in the limiting groove 227 to achieve circumferential limiting between the driving rod 226 and the second rotating rod 224. In this way, the driving rod 226 and the second rotating rod 224 can rotate synchronously and slide relative to each other in the axial direction, so that the two actions of pressing the driving rod 226 to release the lock and rotating the driving rod 226 to fine-tune the angle can be performed independently and smoothly.

[0031] In use, the operator first inserts the culture tube into the placement hole of the waist support 112. The adaptive expansion ring 117 adaptively clamps the outer wall of the culture tube, and the top support 113 limits the upper part of the culture tube. Then, according to the tilt angle requirement of the current process, the operator pulls up the handle 212. The coarse adjustment component 21 drives the waist support 112 and the culture tube around the pin 214 to rotate to near the target angle. After releasing the handle 212, the torsion spring 210 automatically locks the coarse adjustment. Then, the operator presses the drive rod 226 to release the fine adjustment lock, rotates the drive rod 226, and finely adjusts the tilt angle of the culture tube to the precise angle according to the angle markings 222. After releasing the drive rod 226, the damping spring assembly 220 resets and locks the fine adjustment. Throughout the adjustment process, the anti-slip pad 115 and the counterweight 116 work together to ensure the stable placement of the culture tube rack. Through the above operations, precise and stable differential tilt angles can be provided for culture tubes in various processes such as cell settling, layered incubation of culture medium, medium replacement and gentle settling, and staining in the dark, ensuring cell viability and the reproducibility of experimental data.

[0032] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A test tube holder for tube method cell culture, characterized by: Includes a support base (111), a bracket assembly, and an adjustment mechanism (2); The bracket assembly includes a waist bracket (112), a top bracket (113) and a connecting rod (114). The connecting rod (114) is fixedly connected between the waist bracket (112) and the top bracket (113). The waist bracket (112) has several placement holes for supporting culture test tubes. The adjustment mechanism (2) connects the support base (111) and the bracket assembly, including a coarse adjustment component (21) and a fine adjustment component (22). The coarse adjustment component (21) is rotatably hinged to the support base (111), which can drive the bracket assembly to rotate and lock in position, thereby achieving a wide range of tilt angle adjustment. The fine adjustment component (22) is set on the coarse adjustment component (21) and is connected to the waist bracket (112) in a transmission manner. It can drive the waist bracket (112) to deflect slightly relative to the coarse adjustment component (21) and lock in position, thereby achieving precise calibration of the tilt angle.

2. The test tube holder for tube method cell culture according to claim 1, wherein: The coarse adjustment component (21) includes a flip frame (211), a guide bracket (213), a support rod (215), a pin (214), and a rotating rod (216). The flip frame (211) is fitted onto the outside of the waist support (112). The guide bracket (213) is fixed to the back of the support base (111). The support rod (215) is located on the side of the flip frame (211), and the bottom end of the support rod (215) is connected to the support base (111) via the pin (214). The seat (111) is rotatably connected; the inner side of the guide bracket (213) is provided with multiple limiting arc-shaped right angle teeth (217), the rotating rod (216) is rotatably inserted into the inside of the flip frame (211) and its two ends extend out of the sides of the frame. The two ends of the rotating rod (216) are respectively provided with limiting arc-shaped right angle teeth (218), and the limiting arc-shaped right angle teeth (218) and the limiting arc-shaped right angle teeth (217) mesh with each other to achieve the flip angle locking.

3. The test tube holder for tube method cell culture according to claim 2, wherein: The coarse adjustment component (21) also includes a handle (212) and a paddle (2111); the handle (212) is fixed to the back of the flip frame (211), and the paddle (2111) is located inside the handle (212) and its end is fixedly connected to the outer surface of the rotating rod (216); the limiting arc-shaped right angle tooth (218) has a cavity (219) inside, the end of the rotating rod (216) extends into the cavity (219), and a torsion spring (210) is provided in the cavity (219). The two ends of the torsion spring (210) are fixedly connected to the inner wall of the cavity (219) and the end of the rotating rod (216) respectively, and are used to drive the limiting arc-shaped right angle tooth (218) to reset and engage.

4. The test tube holder for tube method cell culture according to claim 2, wherein: The fine-tuning assembly (22) includes a drive rod (226), a second rotating rod (224), a locking disc (228), a damping spring assembly (220), and a limiting disc (2211). The second rotating rod (224) is fixedly connected to the waist support (112). The drive rod (226) and the second rotating rod (224) are coaxially arranged, and the two slide together and can rotate synchronously. The locking disc (228) is fixed on the drive rod (226). The damping spring assembly (220) is located between the locking disc (228) and the limiting disc (2211). The two ends of the damping spring assembly (220) are fixedly connected to the locking disc (228) and the limiting disc (2211) respectively. The limiting disc (2211) is rotatably connected to the support rod (215).

5. The test tube holder for tube method cell culture according to claim 4, wherein: The fine adjustment component (22) also includes an indicator sleeve (221) and a locking block (229); the indicator sleeve (221) is sleeved on the outside of the drive rod (226), and the outer side of the indicator sleeve (221) is provided with angle markings (222) and multiple locking holes (223); the locking plate (228) is fixedly provided with a locking block (229), and the locking block (229) can be inserted into the locking hole (223) to achieve angle positioning; the outer surface of the rotating rod (224) is provided with a limiting protrusion (225) along the axial direction, and the inner wall of the drive rod (226) is provided with a matching limiting groove (227), and the limiting protrusion (225) slides into the limiting groove (227) to achieve circumferential limiting.

6. The test tube rack for single-tube cell culture according to claim 1, characterized in that: Each of the placement holes has an adaptive expansion ring (117) on its inner wall, which is used to adaptively clamp culture tubes with different outer diameters.

7. The test tube holder for tube method cell culture according to claim 1, wherein: The support base (111) is provided with multiple anti-slip pads (115) and counterweights (116) at the bottom. The counterweights (116) are used to lower the overall center of gravity of the frame. The top bracket (113) is provided with limiting holes that correspond one-to-one with the placement holes, which are used to limit the upper part of the culture tube.

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