A separation device for Bacillus fermentation

Abstract

This utility model discloses a separation device for Bacillus fermentation, including a main tank, a separation tank on one side of the main tank, a centrifugal separation component installed inside the main tank, an enzyme addition port fixedly connected to one side of the top of the main tank, the enzyme addition port being equipped with a sealing cap, a side end cap on one side of the separation tank, and a filter component fixedly connected to the side end cap near the separation tank, the filter component including a ceramic membrane, a polyamide nanofiltration membrane, and a polysaccharide separation membrane. This device addresses the problems of high viscosity, excessive foaming, and polysaccharide interference with bacterial growth in Bacillus fermentation broth by adding an enzyme addition port and a stirring component. Polysaccharide-degrading enzymes can be added through the enzyme addition port, and the stirring component ensures thorough mixing of the enzymes with the fermentation broth, efficiently degrading viscous polysaccharides and reducing system viscosity. An antifoaming agent addition port at the top of the separation tank allows for the addition of antifoaming agent as needed, inhibiting foaming in the fermentation broth, improving the bacterial growth environment, and helping to increase the bacterial count to the conventional level of 5000-6000 billion CFU / g.

Description

Technical Field

[0001] This utility model relates to the field of microbial isolation, and in particular to a separation device for Bacillus fermentation. Background Technology

[0002] Bacillus, as an important type of industrial microorganism, is widely used in food, feed, agriculture, medicine, environmental protection and other fields. Its fermentation process usually produces target products, such as enzyme preparations (such as amylase, protease, cellulase, etc.), biopesticides, biofertilizers, antibiotics or their metabolites.

[0003] After fermentation, the target product or target microbial cells or their structural components, such as spores, need to be separated and purified from the complex fermentation broth, which is usually a complex mixture containing water, target product, unused culture medium components, metabolic byproducts, cell bodies (vegetative cells or spores), proteins, polysaccharides, pigments, and various ions and impurities.

[0004] Bacillus fermentation easily produces a large amount of viscous polysaccharides, resulting in high viscosity and excessive foaming in the fermentation broth. This not only affects the growth environment of the strain and limits the increase in bacterial count (conventional Bacillus can reach 500-600 billion CFU / g, while strains affected by polysaccharides can only reach 300+ billion CFU / g), but also increases the difficulty of subsequent separation. The separation and purification of Bacillus fermentation products such as bacterial cells, proteases, and metabolites are key steps in determining product quality. Especially in enzymatic hydrolysis systems, the fermentation broth is more complex due to the presence of viscous polysaccharides, containing bacterial cell debris, small enzymatic molecules, undegraded substrates, and highly viscous polysaccharides. Traditional single separation methods cannot accurately meet the needs of multi-component separation and viscosity reduction. Single centrifugation can only remove large particle residues and cannot separate viscous polysaccharides or reduce the viscosity of the system. Single filtration is prone to clogging of the filter membrane due to the viscosity of polysaccharides and cannot effectively separate polysaccharides, resulting in the loss of target bacterial cells encapsulated by polysaccharides, with extremely low yield, and at the same time, the growth of the strain is limited.

[0005] To address these issues, we propose a separation device for Bacillus fermentation. Utility Model Content

[0006] The purpose of this invention is to provide a separation device for Bacillus fermentation to solve the problems mentioned in the background art.

[0007] To achieve the above objectives, this utility model provides the following technical solution:

[0008] A separation device for Bacillus fermentation includes a main tank, a separation tank on one side of the main tank, a centrifugal separation component installed inside the main tank, an enzyme addition port fixedly connected to one side of the top of the main tank, the enzyme addition port being equipped with a sealing cap, a side end cap on one side of the separation tank, and a filter component fixedly connected to the side end cap near the separation tank, the filter component including a ceramic membrane, a polyamide nanofiltration membrane, and a polysaccharide separation membrane, the ceramic membrane being located above the polyamide nanofiltration membrane, and the polysaccharide separation membrane being located above the ceramic membrane.

[0009] Two locking bolt seats are fixedly connected to the upper surface of the side end cover, and each locking bolt seat is provided with a locking bolt inside. A locking hole adapted to the locking bolt is opened on one side of the separation tank. Two positioning protrusions are fixedly connected to the outer surface of the side end cover. Two positioning slots adapted to the positioning protrusions are fixedly connected to the outer surface of the separation tank. A sealing top cover is provided at the top of the main tank body, and a top cover handle is provided on the upper surface of the sealing top cover.

[0010] The inner wall of the main tank is provided with a stirring assembly, which includes a stirring motor, a stirring shaft and a stirring paddle. The stirring motor is fixed to the upper surface of the sealed top cover, the stirring shaft passes through the sealed top cover and is connected to the output end of the stirring motor, and the stirring paddle is fixed to the bottom of the stirring shaft.

[0011] In a further embodiment, a booster pump is provided between the main tank and the centrifugal separation component. The output end of the booster pump is fixedly connected to a conveying pipe, which is connected to the separation tank. The input end of the booster pump is connected to the bottom end of the main tank.

[0012] In a further embodiment, the bottom end of the separator is fixedly connected to a discharge pipe, the inner wall of the separator is fixedly connected to a guide plate, a sealing ring is provided on the side of the side end cover near the separator, and a defoamer addition port is provided at the top of the separator, the defoamer addition port being equipped with a sealing plug.

[0013] In a further embodiment, the centrifugal separation assembly includes a support frame fixedly connected to the main tank and a protective shell fixedly connected to the support frame. A centrifugal drive motor is fixedly connected to the inner wall of the protective shell, and a centrifugal drum is fixedly connected to the output end of the centrifugal drive motor. An anti-stick coating is provided on the inner wall of the centrifugal drum.

[0014] In a further embodiment, the centrifugal separation assembly further includes a fixed ring rail fixedly connected to the main tank body, and a stabilizing collar is rotatably connected inside the fixed ring rail, the inner ring of which is connected to the top of the centrifugal separation assembly.

[0015] In a further embodiment, the outer surface of the separation tank is fixedly connected with multiple reinforcing legs.

[0016] In a further embodiment, the polysaccharide separation membrane is a hydrophilic ultrafiltration membrane with a pore size of 10-50 kDa, specifically designed to retain viscous polysaccharides; the ceramic membrane has a pore size of 0.1-1 μm, designed to retain fine impurities and denatured enzyme proteins; and the polyamide nanofiltration membrane has a pore size of 1-10 nm, designed to precisely retain target bacterial cells while allowing small molecule impurities to pass through.

[0017] Compared with the prior art, the beneficial effects of this utility model are:

[0018] I. This device addresses the issues of high viscosity, excessive foaming, and polysaccharide interference with bacterial growth in Bacillus fermentation broth by adding an enzyme addition port and a stirring component. Polysaccharide-degrading enzymes (such as glucanase and mannanase) can be added through the enzyme addition port, and the stirring component ensures thorough mixing of the enzymes with the fermentation broth, efficiently degrading viscous polysaccharides and reducing system viscosity. The defoamer addition port at the top of the separation tank allows for the addition of defoamer as needed to suppress foaming in the fermentation broth, improve the bacterial growth environment, and help increase the bacterial count to the conventional level of 5000-6000 billion CFU / g.

[0019] II. This device uses a centrifugal separation component inside the main tank to rotate a centrifugal drum with an anti-stick coating, gently removing bacterial residues, large particles of substrate, and some undegraded polysaccharides from the enzymatic hydrolysis system, preventing the target bacteria from being encapsulated and lost. A booster pump pressurizes and delivers the centrifugal supernatant to the separation tank. The polysaccharide separation membrane of the filtration component first accurately retains the remaining viscous polysaccharides, the ceramic membrane retains fine impurities and denatured enzyme proteins, and the polyamide nanofiltration membrane accurately retains the target bacteria, achieving graded separation of polysaccharides, impurities, and bacteria, significantly improving the bacterial yield and solving the problems of traditional equipment being unable to separate viscous polysaccharides and having limited bacterial growth.

[0020] Third, this device can be quickly disassembled via the side end cap and locking bolts, which facilitates the collection of target bacteria trapped on the filter components. It also facilitates the thorough cleaning and replacement of the filter components, avoiding filter membrane clogging caused by polysaccharide residue. The sealing design and anti-stick coating of each component further reduce contamination and material loss, ensuring separation efficiency and bacterial purity. Attached Figure Description

[0021] Figure 1 A frontal three-dimensional schematic diagram of a separation device for Bacillus fermentation;

[0022] Figure 2 This is a schematic diagram showing the separation of the separation tank and the side end cap in a separation device for Bacillus fermentation.

[0023] Figure 3 This is a frontal cross-sectional view of the separation tank in the separation equipment for Bacillus fermentation;

[0024] Figure 4This is a cross-sectional view of the main tank in the separation equipment for Bacillus fermentation.

[0025] In the diagram: 1. Main tank; 2. Centrifugal separation assembly; 21. Support frame; 22. Protective shell; 23. Centrifugal drive motor; 24. Centrifugal drum; 25. Stirring assembly; 251. Stirring motor; 252. Stirring shaft; 253. Stirring paddle; 20. Enzyme addition port; 3. Fixed ring rail; 4. Stabilizing collar; 5. Booster pump; 6. Sealed top cover; 7. Top cover handle; 8. Delivery pipe; 9. Separation tank; 10. Reinforced support legs; 11. Discharge pipe; 12. Side end cover; 13. Filter assembly; 131. Ceramic membrane; 132. Polyamide nanofiltration membrane; 133. Polysaccharide separation membrane; 14. Locking bolt seat; 15. Locking bolt; 16. Locking hole; 17. Positioning protrusion; 18. Guide plate; 19. Positioning slot; 26. Defoamer addition port. Detailed Implementation

[0026] In the description of this utility model, it should be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientation or positional relationships based on the orientation or positional relationships shown in the accompanying drawings, are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, features defined with "first," "second," etc., may explicitly or implicitly include one or more of that feature. In the description of this utility model, unless otherwise stated, "a plurality of" means two or more.

[0027] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0028] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0029] Please see Figures 1-4In this utility model, a separation device for Bacillus fermentation includes a main tank 1, a separation tank 9 on one side of the main tank 1, a centrifugal separation component 2 installed inside the main tank 1, an enzyme addition port 20 fixedly connected to one side of the top of the main tank 1, the enzyme addition port 20 being equipped with a sealing cap, a side end cap 12 on one side of the separation tank 9, and a filter component 13 fixedly connected to the side end cap 12 near the separation tank 9, the filter component 13 including a ceramic membrane 131, a polyamide nanofiltration membrane 132, and a polysaccharide separation membrane 133, the ceramic membrane 131 being located above the polyamide nanofiltration membrane 132, and the polysaccharide separation membrane 133 being located below the ceramic membrane 131. Above, two locking bolt seats 14 are fixedly connected to the upper surface of the side end cover 12. Each locking bolt seat 14 is provided with a locking bolt 15 inside. One side of the separation tank 9 is provided with a locking hole 16 that matches the locking bolt 15. Two positioning protrusions 17 are fixedly connected to the outer surface of the side end cover 12. Two positioning slots 19 that match the positioning protrusions 17 are fixedly connected to the outer surface of the separation tank 9. A sealing top cover 6 is provided at the top of the main tank body 1. A top cover handle 7 is provided on the upper surface of the sealing top cover 6. A stirring assembly 25 is provided on the inner wall of the main tank body 1. The stirring assembly 25 includes a stirring motor 251, a stirring shaft 252, and a stirring plate. The mixing paddle 253 and the stirring motor 251 are fixed to the upper surface of the sealed top cover 6. The stirring shaft 252 passes through the sealed top cover 6 and is connected to the output end of the stirring motor 251. The mixing paddle 253 is fixed to the bottom of the stirring shaft 252. The filter assembly 13 innovatively adds a polysaccharide separation membrane 133, which, together with the original ceramic membrane 131 and polyamide nanofiltration membrane 132, forms a three-stage filtration system for polysaccharides, impurities, and bacteria. The polysaccharide separation membrane 133 is located on the top layer and can preferentially retain viscous polysaccharides. The ceramic membrane 131 is next, retaining fine impurities and denatured enzyme proteins. The polyamide nanofiltration membrane 132 precisely retains target bacteria. The three-stage filtration not only avoids polysaccharide clogging of subsequent filter membranes, but also... This design reduces the loss of bacteria due to polysaccharide encapsulation and significantly improves bacterial purity, overcoming the shortcomings of traditional equipment such as insufficient targeting and low yield. Combined with the stirring assembly 25 on the inner wall of the main tank 1, the stirring motor 251 drives the stirring shaft 252 and stirring paddle 253 to rotate, ensuring thorough mixing of the enzyme and fermentation broth. This efficiently degrades the viscous polysaccharides produced during fermentation, reducing the viscosity of the fermentation broth and minimizing foaming. This design directly improves the bacterial growth environment, overcoming the limitations imposed by viscous polysaccharides on Bacillus reproduction, and helping to increase the bacterial count from over 300 billion CFU / g to a standard high-quality level of 500-600 billion CFU / g.

[0030] A booster pump 5 is installed between the main tank 1 and the centrifugal separation component 2. The output end of the booster pump 5 is fixedly connected to a delivery pipe 8, which is connected to the separation tank 9. The input end of the booster pump 5 is connected to the bottom end of the main tank 1. The booster pump 5 between the main tank 1 and the separation tank 9 provides stable power for the delivery of the fermentation broth after enzymatic hydrolysis, ensuring that the supernatant after centrifugation is completely delivered to the separation tank 9, reducing the residue of target bacteria. The pressurized delivery causes the supernatant to flow through the filter component 13 at a stable flow rate, improving the filtration efficiency and shortening the separation time. The delivery pipe 8 is sealed to prevent the supernatant from contacting air and protect the activity of the bacteria.

[0031] The bottom of the separator 9 is fixedly connected to a discharge pipe 11. The inner wall of the separator 9 is fixedly connected to a guide plate 18. A sealing ring is provided on the side of the side cover 12 near the separator 9. The top of the separator 9 is provided with a defoamer addition port 26, which is equipped with a sealing plug. The guide plate 18 on the inner wall of the separator 9 guides the fermentation liquid to flow evenly along the tank wall through the filter assembly 13, avoiding local turbulence caused by direct impact of liquid on the filter membrane, protecting the activity of the cells, and preventing local blockage of the filter membrane. The sealing ring of the side cover 12 enhances the sealing performance and prevents external impurities from mixing in. The defoamer addition port 26 can add defoamer as needed to inhibit foaming of the fermentation liquid and improve the separation environment. The discharge pipe 11 at the bottom of the separator 9 facilitates the centralized collection of small molecule impurities in the filtrate.

[0032] The centrifugal separation component 2 includes a support frame 21 fixedly connected to the main tank 1 and a protective shell 22 fixedly connected to the support frame 21. A centrifugal drive motor 23 is fixedly connected to the inner wall of the protective shell 22. A centrifugal drum 24 is fixedly connected to the output end of the centrifugal drive motor 23. The inner wall of the centrifugal drum 24 is provided with an anti-stick coating. The support frame 21 and the protective shell 22 of the centrifugal separation component 2 provide stable support for the centrifugal drive motor 23, ensuring stable operation of the motor. The centrifugal drive motor 23 drives the centrifugal drum 24 to rotate at high speed, generating stable centrifugal force, which efficiently removes bacterial residues, large particles of substrate and some undegraded polysaccharides from the fermentation broth. The anti-stick coating on the inner wall of the centrifugal drum 24 can prevent sticky polysaccharides from adhering to the bacterial cells and reduce material loss. A sterile shaft seal is provided at the drive shaft of the centrifugal drive motor 23 on the outer surface of the protective shell 22 to ensure sealing and smooth rotation.

[0033] The centrifugal separation component 2 also includes a fixed ring rail 3 fixedly connected inside the main tank 1. A stabilizing collar 4 is rotatably connected inside the fixed ring rail 3. The inner ring of the stabilizing collar 4 is connected to the top of the centrifugal separation component 2. The fixed ring rail 3 and the stabilizing collar 4 cooperate to form a stable support for the top of the centrifugal drum 24. Together with the support frame 21, it achieves double fixation from top to bottom, ensuring that there is no eccentric sway when the centrifugal drum 24 rotates at high speed, and avoiding the generation of shear force that damages the bacteria.

[0034] Multiple reinforcing legs 10 are fixedly connected to the outer surface of the separator 9. The multiple reinforcing legs 10 are evenly distributed to provide stable support for the separator 9, avoid the risk of tipping over due to the shift of the center of gravity after the fermentation liquid is full, and ensure production safety.

[0035] The polysaccharide separation membrane 133 is a hydrophilic ultrafiltration membrane with a pore size of 10-50 kDa, specifically designed to retain viscous polysaccharides; the ceramic membrane 131 has a pore size of 0.1-1 μm, retaining fine impurities and denatured enzyme proteins; the polyamide nanofiltration membrane 132 has a pore size of 1-10 nm, precisely retaining target bacteria while allowing small molecule impurities to pass through. The three membranes form a hierarchical filtration system. The polysaccharide separation membrane 133 first separates viscous polysaccharides, solving the core problem of high viscosity; the ceramic membrane 131 protects the subsequent nanofiltration membrane, extending its service life; and the polyamide nanofiltration membrane 132 precisely retains target bacteria, improving bacterial yield and purity.

[0036] The working principle of this utility model is as follows:

[0037] Place the main tank 1 and the separation tank 9 on a level surface in the workshop and secure them with the reinforcing feet 10. Open the sealed top cover 6 and pour the Bacillus fermentation broth into the main tank 1. Depending on the viscosity of the fermentation broth, add an appropriate amount of polysaccharide-degrading enzyme through the enzyme addition port 20. Tighten the sealing cap of the enzyme addition port 20 and the sealed top cover 6. Start the stirring motor 251 of the stirring assembly 25 to drive the stirring shaft 252 and the stirring paddle 253 to rotate, so that the polysaccharide-degrading enzyme and the fermentation broth are fully mixed, degrading the viscous polysaccharides and reducing the viscosity of the system. If the fermentation broth foams severely, add an appropriate amount of defoamer through the defoamer addition port 26 at the top of the separation tank 9 to suppress foaming.

[0038] Start the centrifugal drive motor 23 of the centrifugal separation component 2 to drive the centrifugal drum 24 to rotate at high speed. Under the constraint of the fixed ring rail 3 and the stabilizing collar 4, it runs stably and uses centrifugal force to remove bacterial residue, large particles of substrate and some undegraded polysaccharides from the fermentation broth. After centrifugation is completed, turn off the motor and wait for the drum to stop rotating. Start the booster pump 5 to pressurize and transport the centrifugal supernatant in the main tank 1 to the separation tank 9 through the delivery pipe 8.

[0039] After the supernatant enters the separation tank 9, it is guided by the guide plate 18 and evenly distributed along the tank wall, flowing sequentially through the polysaccharide separation membrane 133, the ceramic membrane 131 and the polyamide nanofiltration membrane 132 of the filter assembly 13: the polysaccharide separation membrane 133 retains the remaining viscous polysaccharides, the ceramic membrane 131 retains fine impurities and denatured enzyme proteins, and the polyamide nanofiltration membrane 132 precisely retains the target bacteria. Small molecule impurities pass through the nanofiltration membrane with the filtrate. The valve of the discharge pipe 11 is opened to collect the filtrate containing small molecule impurities, while the target bacteria remain on the surface of the polyamide nanofiltration membrane 132.

[0040] Close the valves of the booster pump 5 and the discharge pipe 11, unscrew the locking bolt 15, and remove the side end cap 12 by using the cooperation of the positioning protrusion 17 and the positioning slot 19. Rinse the target bacteria trapped on the filter assembly 13 with a small amount of buffer solution and collect them into the product container to complete the bacterial collection. Place the removed filter assembly 13 into the cleaning tank and rinse the polysaccharide trapped on the surface of the polysaccharide separation membrane 133 with clean water. Then rinse the ceramic membrane 131 and the polyamide nanofiltration membrane 132 to remove impurities. After cleaning, put the filter assembly 13 back into the side end cap 12, align it with the positioning protrusion 17 and the positioning slot 19, tighten the locking bolt 15 to reset it, and wait for the next use.

[0041] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0042] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A separation device for Bacillus fermentation, characterized in that: The system includes a main tank (1), a separation tank (9) is provided on one side of the main tank (1), a centrifugal separation component (2) is installed inside the main tank (1), an enzyme addition port (20) is fixedly connected to one side of the top of the main tank (1), the enzyme addition port (20) is equipped with a sealing cap, a side end cap (12) is provided on one side of the separation tank (9), a filter component (13) is fixedly connected to the side of the side end cap (12) near the separation tank (9), the filter component (13) includes a ceramic membrane (131), a polyamide nanofiltration membrane (132) and a polysaccharide separation membrane (133), the ceramic membrane (131) is located above the polyamide nanofiltration membrane (132), and the polysaccharide separation membrane (133) is located above the ceramic membrane (131); The upper surface of the side end cover (12) is fixedly connected to two locking bolt seats (14), and each locking bolt seat (14) is provided with a locking bolt (15). One side of the separation tank (9) is provided with a locking hole (16) that matches the locking bolt (15). The outer surface of the side end cover (12) is fixedly connected to two positioning protrusions (17). The outer surface of the separation tank (9) is fixedly connected to two positioning slots (19) that match the positioning protrusions (17). The top of the main tank (1) is provided with a sealing top cover (6), and the upper surface of the sealing top cover (6) is provided with a top cover handle (7). The inner wall of the main tank (1) is provided with a stirring assembly (25). The stirring assembly (25) includes a stirring motor (251), a stirring shaft (252) and a stirring paddle (253). The stirring motor (251) is fixed to the upper surface of the sealed top cover (6). The stirring shaft (252) passes through the sealed top cover (6) and is connected to the output end of the stirring motor (251). The stirring paddle (253) is fixed to the bottom of the stirring shaft (252).

2. The separation device for Bacillus fermentation according to claim 1, characterized in that: A booster pump (5) is provided between the main tank (1) and the centrifugal separation component (2). The output end of the booster pump (5) is fixedly connected to a conveying pipe (8). The conveying pipe (8) is connected to the separation tank (9). The input end of the booster pump (5) is connected to the bottom end of the main tank (1).

3. The separation device for Bacillus fermentation according to claim 1, characterized in that: The bottom end of the separator (9) is fixedly connected to a discharge pipe (11), the inner wall of the separator (9) is fixedly connected to a guide plate (18), the side end cover (12) is provided with a sealing ring on the side near the separator (9), the top end of the separator (9) is provided with a defoamer addition port (26), and the defoamer addition port (26) is equipped with a sealing plug.

4. The separation device for Bacillus fermentation according to claim 1, characterized in that: The centrifugal separation assembly (2) includes a support frame (21) fixedly connected to the main tank (1) and a protective shell (22) fixedly connected to the support frame (21). A centrifugal drive motor (23) is fixedly connected to the inner wall of the protective shell (22). A centrifugal drum (24) is fixedly connected to the output end of the centrifugal drive motor (23). An anti-stick coating is provided on the inner wall of the centrifugal drum (24).

5. The separation device for Bacillus fermentation according to claim 4, characterized in that: The centrifugal separation assembly (2) also includes a fixed ring rail (3) fixedly connected to the main tank (1), and a stabilizing collar (4) is rotatably connected inside the fixed ring rail (3), with the inner ring of the stabilizing collar (4) connected to the top of the centrifugal separation assembly (2).

6. The separation device for Bacillus fermentation according to claim 1, characterized in that: The outer surface of the separation tank (9) is fixedly connected with multiple reinforcing legs (10).

7. The separation device for Bacillus fermentation according to claim 1, characterized in that: The polysaccharide separation membrane (133) is a hydrophilic ultrafiltration membrane with a pore size of 10-50 kDa, specifically designed to retain viscous polysaccharides. The ceramic membrane (131) has a pore size of 0.1-1 μm, which retains fine impurities and denatured enzyme proteins. The polyamide nanofiltration membrane (132) has a pore size of 1-10 nm, which precisely retains target bacteria while allowing small molecule impurities to pass through.

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