A swing-type thermostatic water bath for cell resuscitation

Abstract

This utility model relates to the field of cell resuscitation technology, specifically a swing-type constant temperature water bath for cell resuscitation. When the heating device starts working and the temperature is constant at the set temperature, the cryopreservation tube is placed into a fixing ring. The inner diameter of the fixing ring is fixed, and a rubber ring is provided inside the fixing ring. Due to the deformation capability of the rubber ring, the cryopreservation tube is inserted into the fixing ring, and the compression of the rubber ring clamps the cryopreservation tube, ensuring a more secure fixation. The swing controller is activated, utilizing a left-right swing structure. This structure converts the rotational motion of a geared motor into left-right swinging motion. The geared motor is a commercially available product. The geared motor is connected to a turntable, and then to a joint bearing, bearing seat, and other parts. The swing is smooth, and the swing amplitude can be adjusted through the waist hole of the turntable. The fixing ring for fixing the cryopreservation tube is set on a sliding rod, achieving a swing of approximately 30° for the cryopreservation tube, which helps improve the thawing efficiency of the cryoprotectant inside the cryopreservation tube.

Description

Technical Field

[0001] This utility model relates to the field of cell resuscitation technology, and in particular to a swing-type constant temperature water bath for cell resuscitation. Background Technology

[0002] Cell resuscitation is the process of thawing cells frozen in liquid nitrogen or at -80°C and then reculturing them to restore cell growth.

[0003] While traditional water bath resuscitation methods are feasible, they suffer from several problems: 1. Traditional methods rely on manually placing cryovials into water for rapid thawing, which is laborious, results in a small number of cells thawed at a time, and is inefficient; 2. The varying water contact heights of each cryovial lead to different thawing times; 3. Cells may clump together during freezing, significantly affecting resuscitation effectiveness and cell viability. Clumped cells are not easily heated evenly during resuscitation, leading to cell damage or incomplete thawing; 4. Inadequate hand hygiene can transfer bacteria, viruses, or other contaminants into the cryovial sample. Therefore, cell resuscitation requires a resuscitation device for batch resuscitation that can restore the state of cells resuscitated manually.

[0004] Therefore, we propose a swing-type constant temperature water bath for cell resuscitation. Utility Model Content

[0005] The purpose of this invention is to address the shortcomings of existing technologies by proposing a swing-type constant-temperature water bath for cell resuscitation. This invention utilizes a left-right swing structure, which converts the rotational motion of a motor into a left-right swing motion. The motor is connected to a turntable, which in turn connects to a joint bearing and other components such as a bearing seat. The swing is smooth, and the amplitude of the swing can be adjusted through the waist hole of the turntable. A fixing ring for securing cryovials is mounted on a sliding rod, allowing for a 30° swing of the cryovials, which helps improve the thawing efficiency of the cryoprotectant inside the cryovials.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A swing-type constant-temperature water bath for cell resuscitation includes a water bath body, a temperature heating device and temperature controller, a swing structure and swing controller, a fixing ring, and a rubber ring. When cells need to be resuscitated in batches, the temperature controller is first set to the required temperature (usually 37℃~56℃), and the heating device starts working, maintaining a constant temperature at the set temperature. Cryopreservation tubes are placed in the fixing ring, which has a fixed inner diameter and contains a rubber ring. Due to the deformability of the rubber ring, inserting the cryopreservation tube into the fixing ring and squeezing the rubber ring clamps the cryopreservation tube, ensuring a more secure fixation. The swing controller is activated, utilizing the left-right swing structure. This structure converts the rotational motion of a motor into left-right swinging motion. The motor is connected to a turntable, and then to joint bearings and bearing seats, etc., ensuring smooth swinging. The swing amplitude can be adjusted through the waist hole of the turntable. The fixing ring for fixing the cryopreservation tubes is set on a sliding rod, achieving a swing of about 30° for the cryopreservation tubes, which helps improve the thawing efficiency of the cryoprotectant inside the cryopreservation tubes. The aforementioned thawing device thaws multiple cryopreservation tubes simultaneously, saving manpower and improving thawing efficiency, while also reducing the risk of contamination from manual operation.

[0008] Compared with the prior art, this utility model provides a swing-type constant temperature water bath for cell resuscitation, which has the following beneficial effects:

[0009] 1. This invention utilizes a swing assembly driven by a geared motor to rotate a turntable, which in turn drives a sliding rod to swing back and forth via a transmission structure including connecting rods and tie rods. This causes the cell containers fixed to the sliding rod to sway evenly in a constant-temperature water bath, preventing localized temperature differences from affecting the resuscitation effect. Simultaneously, the heating device employs a coil design and is equipped with a temperature controller, which precisely maintains the water bath temperature within the set range. This solves the problems of large temperature fluctuations and uneven cell heating in traditional water baths, significantly improving the success rate and consistency of cell resuscitation.

[0010] 2. In this invention, the joint bearings in the swing assembly ensure smooth transmission and reduce mechanical wear, extending the equipment's service life. Multiple retaining rings on the slide bar have built-in rubber rings to securely hold the cryopreservation tubes, preventing them from falling off during shaking. The rubber material also prevents scratch damage caused by direct contact between metal and the container. The integrated control switch is easy to operate and can simultaneously adjust the swing speed and water bath temperature, meeting the needs of efficient laboratory operations and improving the controllability and safety of the cell resuscitation process.

[0011] The parts of this device not covered herein are the same as or can be implemented using existing technologies. This utility model has a simple structure and is easy to operate. Attached Figure Description

[0012] Figure 1This is a schematic diagram of the overall structure of a swing-type constant temperature water bath for cell resuscitation proposed in this utility model.

[0013] Figure 2 This is a side cross-sectional view of the rocking assembly of a rocking constant temperature water bath for cell resuscitation proposed in this utility model.

[0014] Figure 3 This is a three-dimensional structural diagram of a rocking constant temperature water bath slide bar for cell resuscitation proposed in this utility model.

[0015] Figure 4 This is a three-dimensional structural diagram of a swing-type constant temperature water bath for cell resuscitation proposed in this utility model.

[0016] In the diagram: 1. Water bath; 2. Control switch; 3. Water bath lid; 4. Swing assembly; 5. Heating device; 6. Temperature controller; 7. Base; 8. Gear motor; 9. Turntable; 10. Waist hole; 11. Bolt; 12. Connecting rod; 13. Spherical bearing; 14. Pull rod; 15. Bearing; 16. Connecting rod; 17. Slide rod; 18. Bracket; 19. Fixing ring; 20. Rubber ring; 21. Cryopreservation tube. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0018] In the description of this utility model, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0019] Example: A swing-type thermostatic water bath for cell resuscitation, such as... Figures 1-4 As shown, the water bath includes a water bath 1, a control switch 2 is installed on one side of the water bath 1, a water bath 1 lid is movably installed on the upper surface of the water bath 1, a swing assembly 4 is installed inside the water bath 1 near the control switch 2, a heating device 5 is fixedly installed at the bottom inside the water bath 1, and a temperature controller 6 is installed on the side of the water bath 1 near the heating device 5.

[0020] like Figures 1-4As shown, the rocking assembly 4 includes a base 7. The base 7 is fixedly installed inside the water bath 1. A geared motor 8 is fixedly installed on the upper surface of the base 7. The geared motor 8 is electrically connected to the control switch 2. A turntable 9 is fixedly installed at the output end of the geared motor 8. The turntable 9 has a waist hole 10. A connecting rod 12 is fixedly installed inside the waist hole 10 by bolts 11. The connecting rod 12 is curved. A spherical bearing 1513 is movably installed at the end of the connecting rod 12 away from the bolts 11. A pull rod 14 is fixedly installed at the bottom end of the spherical bearing 1513. The bottom end of the pull rod 14 is connected by a shaft. A connecting rod 16 is rotatably connected to the bearing 15. The connecting rod 16 has a hollow interior. A slide rod 17 is fixedly installed at the end of the connecting rod 16 away from the pull rod 14. A bracket 18 is rotatably connected to one end of the slide rod 17. The bracket 18 is fixedly installed inside the water bath 1. The other end of the slide rod 17 is rotatably connected to the side wall of the water bath 1. Five fixing rings 19 are fixedly installed on the outside of the slide rod 17. Rubber rings 20 are fixedly installed inside the fixing rings 19. A cryogenic tube 21 is engaged inside the rubber rings 20. A heating device is fixedly installed below the slide rod 17. The heating device 5 is a coil type and is electrically connected to the temperature controller 6. The device is connected to the laboratory power supply. When cells need to be thawed in batches, first set the temperature controller 6 to the required temperature (usually 37℃~56℃). The heating device 5 will start working and maintain the temperature at the set temperature. Then, place the cryopreservation tubes into the fixing ring 19. The inner diameter of the fixing ring 19 is fixed, and a rubber ring 20 is placed inside the fixing ring 19. Because the rubber ring 20 has deformability, inserting the cryopreservation tubes into the fixing ring 19 and squeezing the rubber ring 20 will allow the cryopreservation tubes to thaw. The cryovial is clamped to ensure a more secure fixation. The swing controller is activated, utilizing a left-right swing structure. This structure converts the rotational motion of the geared motor 8 into a left-right swing motion. The geared motor 8 is a commercially available product. The geared motor 8 is connected to the turntable 9, and then to the joint bearing 1513, as well as the bearing seat 15 and other parts. The swing is smooth, and the swing amplitude can be adjusted through the waist hole 10 of the turntable 9. The fixing ring 19 used to fix the cryovial is set on the slide rod 17 to achieve a 30° left-right swing of the cryovial.

[0021] Working principle: Connect the device to the laboratory power supply. When cells need to be thawed in batches, first set the temperature controller 6 to the required temperature (usually 37℃~56℃). The heating device 5 starts working and the temperature is constant at the set temperature. Place the cryopreservation tube into the fixing ring 19. The inner diameter of the fixing ring 19 is fixed. A rubber ring 20 is set inside the fixing ring 19. Since the rubber ring 20 has the ability to deform, inserting the cryopreservation tube into the fixing ring 19 and squeezing the rubber ring 20 can clamp the cryopreservation tube, ensuring that the cryopreservation tube is more firmly fixed. Turn on the swing controller and use the left and right swing structure. This structure is converted into left and right swinging motion by the rotation of the geared motor 8. The geared motor 8 is a commercially available product. The geared motor 8 is connected to the turntable 9, and then to the joint bearing 1513, as well as the bearing seat 15 and other parts. The swing is stable, and the swing amplitude can be adjusted through the waist hole 10 of the turntable 9. The fixing ring 19 used to fix the cryopreservation tube is set on the slide rod 17. The 30° swing of the cryopreservation tube helps to improve the thawing efficiency of the cryosol in the cryopreservation tube.

[0022] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A swing-type constant-temperature water bath for cell resuscitation, comprising a water bath (1), characterized in that... A control switch (2) is installed on one side of the water bath (1). A water bath (1) lid is movably installed on the upper surface of the water bath (1). A swing assembly (4) is installed inside the water bath (1) near the control switch (2). A heating device (5) is fixedly installed at the bottom inside the water bath (1). A temperature controller (6) is installed on the side of the water bath (1) near the outside and near the heating device (5).

2. The swing-type constant-temperature water bath for cell resuscitation according to claim 1, characterized in that... The swing assembly (4) includes a base (7). The base (7) is fixedly installed inside the water bath (1). A geared motor (8) is fixedly installed on the upper surface of the base (7). The geared motor (8) is electrically connected to the control switch (2). A turntable (9) is fixedly installed at the output end of the geared motor (8). The turntable (9) has a waist hole (10).

3. The swing-type constant-temperature water bath for cell resuscitation according to claim 2, characterized in that... Inside the waist hole (10), a connecting rod (12) is fixedly installed by a bolt (11). The connecting rod (12) is curved. A spherical bearing (15) (13) is movably installed at the end of the connecting rod (12) away from the bolt (11). A pull rod (14) is fixedly installed at the bottom end of the spherical bearing (15) (13). A connecting rod (16) is rotatably connected to the bottom end of the pull rod (14) through the bearing (15).

4. A swing-type constant-temperature water bath for cell resuscitation according to claim 3, characterized in that... The connecting rod (16) is hollow inside. A sliding rod (17) is fixedly installed at one end of the connecting rod (16) away from the pull rod (14). A bracket (18) is rotatably connected to one end of the sliding rod (17). The bracket (18) is fixedly installed inside the water bath (1). The other end of the sliding rod (17) is rotatably connected to the side wall of the water bath (1).

5. A swing-type constant-temperature water bath for cell resuscitation according to claim 4, characterized in that... The slide bar (17) is fixedly installed with five fixing rings (19). A rubber ring (20) is fixedly installed inside the fixing ring (19), and a cryopreservation tube (21) is engaged inside the rubber ring (20).

6. A swing-type constant-temperature water bath for cell resuscitation according to claim 5, characterized in that... A heating device (5) is fixedly installed below the slide rod (17). The heating device (5) is a coil type and is electrically connected to the temperature controller (6).

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