A photoelectric experimental device for hair follicle cell regeneration

Abstract

This invention relates to a photoelectric experimental device for hair follicle cell regeneration, comprising a culture dish (1) containing cell culture medium (2) and a plurality of hair follicle cells (3); a laser module (4) positioned above the culture dish (1) for irradiating the culture dish (1); a microcurrent module (5) positioned above the culture dish (1) with electrode plates extending into the cell culture medium (2) within the culture dish (1); and weak magnetic field modules (6) with N-S magnetic fields positioned on both sides of the culture dish (1), the N and S poles of the weak magnetic field modules (6) being located on opposite sides of the culture dish (1). This experimental device, when combined with physical therapy, can more effectively improve hair growth compared to treatment alone.

Description

Technical Field

[0001] This utility model relates to an experimental device, specifically a photoelectric experimental device for hair follicle cell regeneration. Background Technology

[0002] With the fast pace of modern life and increasing work pressure, hair loss has become a significant issue affecting the health and quality of life of people worldwide. Currently, treatments for hair loss mainly include medication (such as minoxidil and finasteride), hair transplantation, and physical therapy.

[0003] Low-Level Laser Therapy (LLLT), as a non-invasive physical therapy, has been widely used in clinical practice. Its mechanism of action mainly relies on the irradiation of the scalp with a specific wavelength (such as 650nm) of laser light, which promotes the energy metabolism of hair follicle cells through photobiomodulation (PBM), thereby improving hair loss symptoms.

[0004] However, the efficacy of single phototherapy modalities varies from person to person, and some patients report limited effects. Therefore, there is an urgent need to explore more efficient multimodal combined treatment options.

[0005] Recent studies have shown that various physical stimulation methods can promote hair follicle activation and hair regeneration through different molecular mechanisms, including: Low-energy laser therapy (LLLT) mechanism: 650nm laser light can be absorbed by cytochrome C oxidase (CCO), promoting mitochondrial ATP synthesis, enhancing cellular metabolic activity, and reducing oxidative stress, thereby prolonging the hair follicle anagen phase.

[0006] Clinical evidence: Multiple randomized controlled trials (RCTs) have demonstrated that LLLT can significantly improve hair density in patients with androgenetic alopecia (AGA) (such as FDA-approved laser hair growth caps).

[0007] Bipolar biphasic microcurrent stimulation (MSS) utilizes microcurrents (typically in the μA range) to activate the Wnt / β-catenin and MAPK signaling pathways in hair follicle stem cells, promoting hair follicle proliferation and inhibiting apoptosis. Furthermore, the stimulation enhances local blood circulation and improves the hair follicle microenvironment. In vitro experiments have shown that microcurrents can upregulate the expression of VEGF and IGF-1 in dermal papilla cells (DPCs), promoting hair follicle regeneration.

[0008] Pulsed Electromagnetic Field (PEMF) therapy uses electromagnetic fields of specific frequencies to modulate signaling pathways such as GSK-3β / ERK / Akt, promoting the proliferation of keratinocytes (KCs) and dermal papilla cells. Simultaneously, PEMF can induce the secretion of KGF (keratinocyte growth factor), accelerating the transition of hair follicles from the telogen phase to the anagen phase. Animal models have shown that PEMF treatment can significantly increase hair density and shorten the regeneration cycle.

[0009] While each of the aforementioned individual therapies has some efficacy, their combined use may produce synergistic effects due to differences in their target sites and mechanisms of action. However, most commercially available devices are currently limited to a single mode (such as pure phototherapy or pure electrical stimulation), lacking comprehensive treatment devices that integrate multiple stimulations of light, electricity, and magnetism. Utility Model Content

[0010] To address the aforementioned technical problems, the purpose of this invention is to propose a photoelectric experimental device for hair follicle cell regeneration. By simultaneously or sequentially applying LLLT, microcurrent, and PEMF, it synergistically promotes hair follicle regeneration from multiple dimensions, including energy metabolism, signal pathway activation, and cellular microenvironment regulation, thereby improving treatment efficacy and shortening the treatment course.

[0011] The technical solution of this utility model is as follows: A photoelectric experimental device for hair follicle cell regeneration, characterized in that: A cell culture unit includes a culture dish 1, wherein a cell culture medium 2 is disposed in the culture dish 1, and a plurality of hair follicle cells 3 are disposed in the cell culture medium 2; An optical stimulation unit, a laser module 4 disposed above the culture dish 1, is used to irradiate the culture dish 1. An electrical stimulation unit is disposed above the culture dish 1, and a microcurrent module 5 has electrode plates that extend into the cell culture medium 2 inside the culture dish 1. A magnetic stimulation unit is disposed on both sides of the culture dish 1, and a weak magnetic field module 6 with an N-S magnetic field is provided. The N pole and S pole of the weak magnetic field module 6 are located on both sides of the culture dish 1, respectively.

[0012] Furthermore, the laser module 4 includes a PCB board 402 and a plurality of LED beads 401 disposed on the PCB board 402.

[0013] Furthermore, the LED beads 401 are arranged in a rectangular pattern, with four beads in each group.

[0014] Furthermore, the microcurrent module 5 includes multiple electrode plates 501 arranged at equal intervals.

[0015] Furthermore, the weak magnetic field module includes a housing 601, with N poles and S poles of the magnetic field at both ends of the housing 601; a bottom cover 603 is provided at the bottom of the housing 601, and the bottom cover 603 and the housing 601 are connected by bolts 604; and a cooling fan 605 is provided at both ends of the housing 601.

[0016] Furthermore, the cover 601 is also provided with four rectangular corner strips 602, which are used to engage the height block.

[0017] Furthermore, the current flowing through the electrode plate 501 of the microcurrent module 5 is 50–200 μA.

[0018] Furthermore, the wavelength of the light emitted by the lamp bead 401 is 650nm.

[0019] By means of the above solution, this utility model has at least the following advantages: The combination of a laser module, a microcurrent module, and a weak magnetic field module yielded the best experimental results. This is because low-energy laser stimulation promotes the production of more ATP by cytochrome C oxidase (CCO), which in turn promotes cellular metabolism and biosynthesis. Microcurrent stimulation activates signaling pathways such as Wnt / β-catenin and MAPK, thereby stimulating hair follicle proliferation and inducing and prolonging the growth phase. Pulsed electromagnetic field therapy promotes increased KGF expression and activates signaling pathways such as GSK-3β / ERK / Akt, providing more energy for the biological processes regulated by these pathways. Therefore, combined photoelectromagnetic stimulation is more effective than single stimulation in enhancing cell proliferation and repair.

[0020] The above description is only an overview of the technical solution of this utility model. In order to better understand the technical means of this utility model and to implement it in accordance with the contents of the specification, the preferred embodiments of this utility model are described in detail below with reference to the accompanying drawings. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the structure of this utility model; Figure 2 This is a schematic diagram of the structure of the laser module of this utility model; Figure 3 This is a schematic diagram of the microcurrent module of this utility model; Figure 4 This is a schematic diagram of the structure of the weak magnetic field module of this utility model; In the picture: 1- Petri dish; 2-Cell culture medium; 3-hair follicle cells; 4-Laser module; 401-LED chip; 402-PCB board; 5-Microcurrent module; 501-Electrode plate; 6-Weak magnetic field module; 601-Cover; 602-Corner strip; 603-Bottom cover; 604-Bolt; 605-Cooling fan. Detailed Implementation

[0022] The specific embodiments of this utility model will be further described in detail below with reference to the accompanying drawings and examples. The following examples are used to illustrate this utility model, but are not intended to limit the scope of this utility model.

[0023] See Figures 1-4 The preferred embodiment of this utility model describes a photoelectric experimental device for hair follicle cell regeneration, which can simultaneously apply LLLT, microcurrent and PEMF to promote hair follicle regeneration from multiple dimensions such as energy metabolism, signal pathway activation and cell microenvironment regulation, thereby improving the treatment effect and shortening the treatment course.

[0024] The device specifically includes: The apparatus includes a glass culture dish 1 containing a cell culture medium 2 and several hair follicle cells 3. The culture dish 1 is glass in shape, and the culture medium 2 is placed in the culture dish 1. The hair follicle cells 3 are located in the culture medium 2. The experimental device stimulates the hair follicle cells 3 in the culture medium 2 through various stimulation methods to promote their growth.

[0025] Above the culture dish 1, a laser module 4 is provided for irradiating the culture dish 1. The laser module 4 includes a PCB board 402 and multiple LED beads 401 disposed on the PCB board 402. The LED beads 401 are arranged in groups of four in a rectangular configuration. The purpose of this arrangement is to ensure that each group of LED beads 401 corresponds to one culture dish 1, that is, multiple culture dishes 1 can be provided, and the LED beads 401 are semiconductor lasers.

[0026] In the laser module, the wavelength of light emitted by the 401 LED bead is 650nm.

[0027] In the specific experiment, the power emitted by the laser module was approximately 4-6 J / cm². 2 At this energy density, hair follicle cells will not be damaged.

[0028] In the specific experiment, the laser module was irradiated for 15 minutes at a time, and was irradiated only once within 24 hours.

[0029] It also includes a microcurrent module 5 with electrode plates disposed above the culture dish 1, the electrode plates extending into the cell culture medium 2 inside the culture dish 1; the microcurrent module 5 includes a plurality of electrode plates 501 arranged at equal intervals.

[0030] The current intensity supplied to the microcurrent module 5 is 50–200 μA, which promotes hair follicle proliferation (through the Wnt / β-catenin pathway), and the voltage supplied with this current is less than 10V.

[0031] In the microcurrent module 5, the electrode plate 501 is made of glassy carbon electrode, and the distance between two adjacent electrodes is 18 mm. At the same time, the diameter of the culture dish 1 is also 18 mm.

[0032] The microcurrent module 5 has a single power-on time of 15 minutes and is powered on only once within 24 hours.

[0033] The laser module and the microcurrent module 5 can also be combined together. Specifically, the electrode plates 501 can be spaced on the PCB board. The specific arrangement method can be soldering or bonding.

[0034] It also includes a weak magnetic field module 6 with an N-S magnetic field, which is set on both sides of the petri dish 1. The N pole and S pole of the weak magnetic field module 6 are located on both sides of the petri dish 1, respectively.

[0035] The weak magnetic field module includes a housing 601, with N and S poles for the magnetic field at both ends. A bottom cover 603 is located at the bottom of the housing 601, and the bottom cover 603 is connected to the housing 601 by bolts 604. Cooling fans 605 are located at both ends of the housing 601. The housing 601 also has four rectangular corner strips 602, which are used to engage height blocks. The height blocks are used to place the culture dish 1. By adjusting the height of the height blocks, the culture dish 1 is positioned on the spherical surface of the 5mT magnetic field. At a strength of 5mT, KGF in hair follicles can be promoted, the GSK-3β / Akt pathway can be activated, and the hair follicle growth phase can be prolonged.

[0036] The weak magnetic field module 6 has a single power-on time of 15 minutes and is powered on only once within 24 hours.

[0037] The experiment was conducted with the following parameters: the laser module's single irradiation time was 15 minutes, the microcurrent module 5's single power-on time was 15 minutes, and the weak magnetic field module 6's single power-on time was 15 minutes. The laser module's single irradiation time, the microcurrent module's single power-on time, and the weak magnetic field module's single power-on time were combined and compared. The following experimental results were obtained. The results above show that the combination of a laser module, a microcurrent module, and a weak magnetic field module yielded the best experimental results. This is because low-energy laser stimulation promotes the production of more ATP by cytochrome C oxidase (CCO), which in turn promotes cellular metabolism and biosynthesis. Microcurrent stimulation activates signaling pathways such as Wnt / β-catenin and MAPK, thereby stimulating hair follicle proliferation and inducing and prolonging the growth phase. Pulsed electromagnetic field therapy promotes increased KGF expression and activates signaling pathways such as GSK-3β / ERK / Akt, providing more energy for the biological processes regulated by these pathways. Therefore, combined photoelectromagnetic stimulation is more effective than single stimulation in enhancing cell proliferation and repair.

[0038] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. A photoelectromagnetic experimental device for hair follicle cell regeneration, characterized by, include: The cell culture unit includes a culture dish (1), in which cell culture medium (2) is provided, and a number of hair follicle cells (3) are provided in the cell culture medium (2). An optical stimulation unit is disposed above the culture dish (1) and is a laser module (4) used to irradiate the culture dish (1). An electrical stimulation unit is disposed above the culture dish (1) and has a microcurrent module (5) with electrode plates, the electrode plates extending into the cell culture medium (2) inside the culture dish (1); A magnetic stimulation unit is disposed on both sides of the culture dish (1), and a weak magnetic field module (6) with an N-S magnetic field is provided. The N pole and S pole of the weak magnetic field module (6) are located on both sides of the culture dish (1).

2. The photoelectric experimental device for hair follicle cell regeneration according to claim 1, characterized in that: The laser module (4) includes a PCB board (402) and a plurality of LED beads (401) disposed on the PCB board (402).

3. The optoelectromagnetic experimental device for hair follicle cell regeneration according to claim 2, characterized in that: The LED beads (401) are arranged in a rectangular pattern, with four beads per group.

4. The photoelectric experimental device for hair follicle cell regeneration according to claim 1, characterized in that: The microcurrent module (5) includes multiple electrode plates (501) arranged at equal intervals.

5. The photoelectric experimental device for hair follicle cell regeneration according to claim 1, characterized in that: The weak magnetic field module includes a housing (601), with N pole and S pole of magnetic field at both ends of the housing (601); a bottom cover (603) is provided at the bottom of the housing (601), and the bottom cover (603) and the housing (601) are connected by bolts (604); and a cooling fan (605) is provided at both ends of the housing (601).

6. The photoelectric experimental device for hair follicle cell regeneration according to claim 5, characterized in that: The cover (601) is also provided with four rectangular corner strips (602), which are used to engage the height block.

7. The photoelectric experimental device for hair follicle cell regeneration according to claim 1, characterized in that: The current flowing through the electrode plate (501) of the microcurrent module (5) is 50–200 μA.

8. The photoelectric experimental device for hair follicle cell regeneration according to claim 3, characterized in that: The wavelength of light emitted by the lamp bead (401) is 650nm.

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