A cell co-culture collection device

Abstract

This utility model discloses a cell co-culture collection device, belonging to the field of cell co-culture technology, comprising: a culture dish and a fixing component, the fixing component being snapped onto the opening of the culture dish; a polycarbonate membrane, the polycarbonate membrane being installed on the fixing component, used to divide the internal space of the culture dish into two independent spaces for culturing different cells respectively; wherein, the fixing component has a fixing part installed inside, used to clamp and fix the polycarbonate membrane. This utility model consists of a telescopic rod and a clamping part forming the fixing component. The telescopic rod can flexibly adjust the overall length to adapt to culture dishes of different diameters. The distance between the two spring clips is controlled by a sliding plate, thereby controlling the clamping force of the two spring clips on the polycarbonate membrane. The clamping force increases during the culture process to ensure stable culture, and decreases during disassembly and assembly for easy disassembly and assembly.

Description

Technical Field

[0001] This utility model relates to the field of cell co-culture technology, specifically to a cell co-culture collection device. Background Technology

[0002] In cell biology research, cell co-culture technology is a key means to explore the interactions and material exchange between different cells. Its core requirement is to achieve effective isolation and stable culture of different cells, while ensuring the controllability of the cell growth environment.

[0003] In existing co-culture devices, the supports used to fix polycarbonate membranes are mostly one-piece molded structures, whose dimensions are strictly matched with specific-sized culture dishes, and cannot be flexibly adapted to culture dishes of different diameters according to experimental needs.

[0004] Existing polycarbonate membranes used for cell isolation and material exchange require screws, clamps, and other tools to rigidly fix the membrane to the separator. Improper force during installation can easily damage the membrane. Although some devices use elastic clamping structures, the clamping force is uncontrollable. If the clamping force is too small, the membrane is prone to shifting during culture. If the clamping force is too large, it will increase the risk of membrane damage during disassembly and also increase the probability of contamination during operation.

[0005] To address the aforementioned problems, this invention provides a cell co-culture collection device. Utility Model Content

[0006] To address the aforementioned technical shortcomings, the purpose of this utility model is to provide a cell co-culture collection device. The device consists of a telescopic rod and a clamping part forming a fixing assembly. The telescopic rod can flexibly adjust its overall length to adapt to culture dishes of different diameters. The distance between the two spring plates is controlled by a sliding plate, thereby controlling the clamping force of the two spring plates on the polycarbonate membrane. The clamping force increases during the culture process to ensure stable culture, and decreases during disassembly and assembly to facilitate disassembly and assembly.

[0007] To solve the above-mentioned technical problems, the present invention adopts the following technical solution: The present invention provides a cell co-culture collection device, comprising: Petri dish; A fixing component, which is snapped onto the opening of the petri dish; A polycarbonate membrane, which is mounted on a fixing assembly, is used to divide the internal space of a culture dish into two independent spaces for culturing different cells. The fixing component has a fixing part installed inside for clamping and fixing the polycarbonate film.

[0008] Preferably, the fixing component includes: The telescopic rod has a limiting groove on its lower side that is adapted to the polycarbonate film. Two clamping parts are located at the two ends of the lower side of the telescopic rod, respectively, for clamping the side wall of the culture dish. A fixing part is installed inside each of the two clamping parts.

[0009] Preferably, the telescopic rod includes: a first rod body and a second rod body, both of which are provided with limiting grooves. A plug is fixedly installed at one end of the first rod body near the second rod body, and a slot adapted to the plug is provided on the second rod body.

[0010] Preferably, the second rod is threaded with a compression bolt, which extends through the second rod and into the slot to abut against the insert rod.

[0011] Preferably, the clamping part includes: The fixed rod is fixedly installed on the telescopic rod; An elastic unit is fixedly installed on the side of the fixing rod away from the telescopic rod. When the fixing assembly is installed on the culture dish, the elastic unit and the fixing rod cooperate to clamp the side wall of the culture dish.

[0012] Preferably, the elastic unit includes a clamping rod, which is shaped like a "┍". Its horizontal end sidewall is fixedly connected to a fixing rod, and its vertical end sidewall is a hollow structure made of elastic material. The distance between the vertical end sidewall of the clamping rod and the fixing rod is less than the wall thickness of the petri dish.

[0013] Preferably, the fixing part includes: Two spring clips are respectively installed on the two side walls of the empty slot. The empty slot is located on the side of the fixed rod near the telescopic rod and communicates with the limiting slot on the telescopic rod. The two spring clips are arc-shaped and their openings face away from each other. When the polycarbonate film is inserted into the empty slot, the two spring clips cooperate to clamp and fix the polycarbonate film.

[0014] Preferably, the end of the spring piece near the opening of the empty slot is fixedly connected to the wall of the empty slot, and the end away from the opening of the empty slot is a floating end, which is slidably connected to the wall of the empty slot.

[0015] Preferably, a sliding plate is slidably installed between the floating end of the spring sheet and the bottom sidewall of the empty slot. An extrusion plate is fixedly installed on the side of the sliding plate near the bottom sidewall of the empty slot. The extrusion plate extends through the bottom sidewall of the empty slot to the outside of the fixed rod. The lower outer end of the extrusion plate is wedge-shaped, narrow at the bottom and wide at the top.

[0016] Preferably, push blocks are slidably installed on both the first rod and the second rod. One side of the push block extends into the limiting groove and folds to both sides to form a boss, while the other side extends to the outside of the first rod and the second rod and folds to all sides to form a boss.

[0017] The beneficial effects of this utility model are as follows: This invention, through the design of a culture dish, a fixing component with a fixing part, and a polycarbonate membrane, achieves stable installation by having the clamping part of the fixing component snap into the opening of the culture dish, and the fixing part reliably clamps the polycarbonate membrane, allowing the polycarbonate membrane to precisely divide the culture dish into two independent spaces. At the same time, the first and second rods of the telescopic rod are locked together by the cooperation of the insertion rod, slot, and compression bolt, and the overall length can be flexibly adjusted to adapt to culture dishes of different diameters. This solves the problem that traditional fixing partitions can only adapt to a single size of culture dish and are prone to loosening after installation. It achieves stable cell co-culture partitioning under culture dishes of different sizes, ensuring independent growth of the two types of cells and controllable material exchange.

[0018] This invention, through the arrangement of the spring sheet, sliding plate, extrusion plate, and push block on the telescopic rod of the fixing part, enables the spring sheet with opposite arc-shaped openings to automatically and elastically clamp the polycarbonate film during installation. When the fixing component is installed inside the petri dish, the side wall of the petri dish presses the extrusion plate against the interior of the slot, thereby allowing the sliding plate to push the floating end of the spring sheet, shortening the distance between the two ends of the spring sheet, and thus reducing the distance between the two spring sheets, thereby more stably clamping the polycarbonate film. When the fixing component is removed from the petri dish, the sliding plate no longer pushes the floating end of the spring sheet, and the distance between the two spring sheets increases, making it easier for workers to install and remove the polycarbonate film. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 This is a schematic diagram of a cell co-culture collection device provided in an embodiment of the present invention.

[0021] Figure 2 This is an exploded view of the present invention.

[0022] Figure 3 This is an exploded view of the polycarbonate film, the first rod, and the second rod of this utility model.

[0023] Figure 4 This is an exploded view of the first rod and the second rod of this utility model.

[0024] Figure 5 This is a cross-sectional view of the first and second rods of this utility model.

[0025] Figure 6 This is a schematic diagram of the installation of the spring clip of this utility model.

[0026] Explanation of reference numerals in the attached figures: 1. Petri dish, 2. Polycarbonate film, 3. First rod, 4. Second rod, 5. Insert rod, 6. Slot, 7. Fixing rod, 8. Clamping rod, 9. Spring, 10. Empty slot, 11. Slide plate, 12. Extrusion plate, 13. Push block. Detailed Implementation

[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0028] This invention provides a cell co-culture collection device, such as... Figures 1 to 6 As shown.

[0029] Example 1: A cell co-culture collection device includes a culture dish 1, which serves as the basic carrier for cell culture. A fixing component is snapped onto the opening of the culture dish 1, and a polycarbonate membrane 2 is installed on the fixing component. When the fixing component is installed on the culture dish 1, the polycarbonate membrane 2 on it can divide the internal space of the culture dish 1 into two independent spaces for culturing different cells.

[0030] The fixing assembly includes a telescopic rod and two clamping parts. The telescopic rod consists of a first rod body 3 and a second rod body 4. The first rod body 3 has an integrally formed insertion rod 5 at one end near the second rod body 4. The second rod body 4 has a slot 6 along its length that matches the size of the insertion rod 5. The insertion rod 5 can slide along the long side of the slot 6 to adjust the overall length of the telescopic rod. At the same time, the lower sides of both the first rod body 3 and the second rod body 4 have limiting grooves that are adapted to the polycarbonate film 2, providing a positioning basis for the subsequent installation of the polycarbonate film 2.

[0031] The second rod 4 has a threaded hole on its side wall that communicates with the slot 6. A compression bolt is threaded into the threaded hole. When the length of the telescopic rod is adjusted to match the diameter of the culture dish 1, the compression bolt is rotated clockwise so that the end of the compression bolt passes through the second rod 4 and extends into the slot 6, and abuts tightly against the outer wall of the insertion rod 5. The friction force restricts the sliding of the insertion rod 5 in the slot 6, thereby locking the length of the telescopic rod.

[0032] The length of the telescopic rod is adjusted by sliding the insertion rod 5 and the slot 6, and then locked by the compression bolt, so that the telescopic rod can be adapted to the opening of the culture dish 1 of different diameters.

[0033] Two clamping parts are located at the lower ends of the telescopic rod and are used to clamp the side wall of the culture dish 1, so that the fixing component is snapped into the opening of the culture dish 1. The clamping parts are equipped with fixing parts inside, which are used to clamp and fix the polycarbonate film 2.

[0034] Example 2: The clamping part includes a fixing rod 7 and an elastic unit. The fixing rod 7 is vertically fixed to the lower end of the telescopic rod (the fixing rod 7 connected to the first rod 3 is located at the end of the first rod 3 away from the second rod 4, and the fixing rod 7 connected to the second rod 4 is located at the end of the second rod 4 away from the first rod 3). The fixing rod 7 is made of medical-grade ABS plastic, which has both rigidity and corrosion resistance. The elastic unit is fixedly installed on the side of the fixing rod 7 away from the telescopic rod, and is used to cooperate with the fixing rod 7 to clamp the side wall of the culture dish 1.

[0035] The elastic unit is a clamping rod 8, which is shaped like a "┍". Its horizontal sidewall is fixedly connected to the sidewall of the fixing rod 7 by adhesive bonding or ultrasonic welding. The vertical sidewall is made of medical elastic silicone and has a hollow structure inside (the hollow design can improve the deformation capacity of the vertical end). At the same time, the initial distance between the vertical sidewall of the clamping rod 8 and the fixing rod 7 is less than the wall thickness of the culture dish 1. When the fixing component is installed at the opening of the culture dish 1, the vertical end of the clamping rod 8 is squeezed by the sidewall of the culture dish 1 and undergoes elastic deformation. The reaction force generated by the deformation causes the vertical end of the clamping rod 8 and the fixing rod 7 to clamp the sidewall of the culture dish 1 together, realizing the snap-fit ​​fixation between the fixing component and the culture dish 1.

[0036] A slot 10 is provided on the side of the fixed rod 7 near the telescopic rod. The slot 10 extends along the height direction of the fixed rod 7, and the top of the slot 10 is connected to the limiting groove on the lower side of the telescopic rod to form a membrane installation channel. Spring pieces 9 are fixedly installed on the two side walls of the slot 10. The spring pieces 9 are arc-shaped and the opening directions of the two spring pieces 9 are opposite (that is, the side near the center of the slot 10 is an arc-shaped convex surface). When the polycarbonate membrane 2 is inserted into the slot 10, the edge of the polycarbonate membrane 2 will squeeze the arc-shaped convex surface of the two spring pieces 9, causing the spring pieces 9 to undergo slight elastic deformation. The clamping force generated by the deformation acts on both sides of the polycarbonate membrane 2, realizing the initial fixation of the polycarbonate membrane 2 and preventing the polycarbonate membrane 2 from shifting laterally in the limiting groove.

[0037] The fixing rod 7 provides a mounting carrier for the clamping rod 8 and the spring piece 9. The elastic deformation of the clamping rod 8 enables the fixing component to be stably clamped to the culture dish 1, preventing the fixing component from falling off from the opening of the culture dish 1. The arc-shaped structure and elastic properties of the spring piece 9 provide flexible clamping force for the polycarbonate film 2, which not only avoids damage to the polycarbonate film 2 caused by rigid clamping, but also restricts the displacement of the polycarbonate film 2, laying the foundation for subsequent precise separation of the space in the culture dish 1.

[0038] Example 3: To address the issues of cumbersome assembly and disassembly and easy damage associated with traditional polycarbonate membranes 2, the spring clip 9 can be installed in the following manner, specifically as follows: One end of the spring 9 near the opening of the empty slot 10 is fixedly connected to the wall of the empty slot 10, and the other end away from the opening of the empty slot 10 is a floating end. A sliding plate 11 is slidably installed between the floating end of the spring 9 and the bottom side wall of the empty slot 10.

[0039] An extrusion plate 12 is fixed to the side of the slide plate 11 near the bottom sidewall of the slot 10 by an integral molding method. The bottom sidewall of the slot 10 has a through hole that matches the extrusion plate 12. The end of the extrusion plate 12 away from the slide plate 11 extends through the through hole to the outside of the fixing rod 7, and the lower end of the part of the extrusion plate 12 extending to the outside is a wedge-shaped structure that is narrow at the bottom and wide at the top.

[0040] When the fixing component is installed into the opening of the culture dish 1, the inner sidewall of the culture dish 1 first contacts the wedge-shaped end of the extrusion plate 12. As the fixing component is lowered, the sidewall of the culture dish 1 generates a horizontal extrusion force on the wedge-shaped end, pushing the extrusion plate 12 into the empty groove 10. The extrusion plate 12 drives the slide plate 11 to slide along the bottom of the empty groove 10. The upper end face of the slide plate 11 contacts the floating end of the spring piece 9 and pushes the floating end to move towards the center of the empty groove 10, thereby reducing the gap between the two spring pieces 9 and thus enhancing the clamping force on the polycarbonate film 2, preventing the polycarbonate film 2 from loosening or leaking during the culture process.

[0041] The upper sides of the first rod 3 and the second rod 4 are provided with strip-shaped grooves. A push block 13 is slidably installed in the groove. One side of the push block 13 extends through the groove into the limiting groove of the telescopic rod and folds to both sides of the limiting groove to form a boss. The boss located inside the limiting groove can increase the contact area with the polycarbonate film 2, thereby better pushing the polycarbonate film 2. The other side of the push block 13 extends to the outside of the first rod 3 or the second rod 4 and folds to all sides to form a boss that is easy to push with fingers.

[0042] When disassembling the polycarbonate membrane 2, first remove the fixing component from the culture dish 1. The extrusion plate 12 loses the extrusion force from the side wall of the culture dish 1, and the floating end of the spring piece 9 resets under its own elasticity, driving the slide plate 11 and the extrusion plate 12 to reset. The distance between the two spring pieces 9 returns to the initial state. At this time, pushing the push block 13 can push the polycarbonate membrane 2 out of the limiting groove without disassembling the fixing component, reducing the risk of damage and contamination to the polycarbonate membrane 2.

[0043] Obviously, those skilled in the art can make various modifications and variations to this utility model without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this utility model and their equivalents, this utility model also intends to include these modifications and variations.

Claims

1. A cell co-culture collection device, characterized in that, include: Petri dish (1); A fixing component is snapped into the opening of the petri dish (1); A polycarbonate membrane (2) is mounted on a fixing assembly to divide the internal space of the culture dish (1) into two independent spaces for culturing different cells. The fixing component has a fixing part installed inside for clamping and fixing the polycarbonate film (2). The fixing component includes: Telescopic rod, the lower side of which is provided with a limiting groove adapted to the polycarbonate film (2); Two clamping parts are located at the two ends of the lower side of the telescopic rod, respectively, for clamping the side wall of the culture dish (1), and a fixing part is installed inside the two clamping parts; The clamping part includes: Fixed rod (7), the fixed rod (7) is fixedly installed on the telescopic rod; The elastic unit is fixedly installed on the side of the fixed rod (7) away from the telescopic rod. When the fixed assembly is installed on the petri dish (1), the elastic unit and the fixed rod (7) cooperate to clamp the side wall of the petri dish. The fixing part includes: Two spring pieces (9) are respectively installed on the two side walls of the empty groove (10). The empty groove (10) is located on the side of the fixed rod (7) near the telescopic rod and communicates with the limiting groove on the telescopic rod. The two spring pieces (9) are arc-shaped and their openings are opposite to each other. When the polycarbonate film (2) is inserted into the empty groove (10), the two spring pieces (9) cooperate with each other to clamp and fix the polycarbonate film (2). The end of the spring piece (9) near the opening of the slot (10) is fixedly connected to the wall of the slot (10), and the end away from the opening of the slot (10) is a floating end, which is slidably connected to the wall of the slot (10). A sliding plate (11) is slidably installed between the floating end of the spring piece (9) and the bottom side wall of the empty groove (10). A pressing plate (12) is fixedly installed on the side of the sliding plate (11) near the bottom side wall of the empty groove (10). The pressing plate (12) extends through the bottom side wall of the empty groove (10) to the outside of the fixing rod (7). The lower outer end of the pressing plate (12) is set as a wedge shape that is narrow at the bottom and wide at the top.

2. The cell co-culture collection device as described in claim 1, characterized in that, The telescopic rod includes a first rod body (3) and a second rod body (4). Both the first rod body (3) and the second rod body (4) are provided with limiting grooves. A plug rod (5) is fixedly installed at one end of the first rod body (3) near the second rod body (4). A slot (6) adapted to the plug rod (5) is provided on the second rod body (4).

3. The cell co-culture collection device as described in claim 2, characterized in that, The second rod (4) is threaded with a compression bolt, which extends through the second rod (4) and into the slot (6) to press against the insert rod (5).

4. The cell co-culture collection device as described in claim 1, characterized in that, The elastic unit includes a clamp (8), which is shaped like a "┍". Its horizontal end sidewall is fixedly connected to the fixing rod (7), and its vertical end sidewall is a hollow structure made of elastic material. The distance between the vertical end sidewall of the clamp (8) and the fixing rod (7) is less than the wall thickness of the petri dish (1).

5. The cell co-culture collection device as described in claim 2, characterized in that, Push blocks (13) are slidably installed on the first rod (3) and the second rod (4). One side of the push block (13) extends into the limiting groove and folds to both sides to form a boss, and the other side extends to the outside of the first rod (3) and the second rod (4) and folds to all sides to form a boss.

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